CN105145641B - A kind of granule containing trichoderma harzianum and chitosan oligosaccharide - Google Patents
A kind of granule containing trichoderma harzianum and chitosan oligosaccharide Download PDFInfo
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- CN105145641B CN105145641B CN201510697559.9A CN201510697559A CN105145641B CN 105145641 B CN105145641 B CN 105145641B CN 201510697559 A CN201510697559 A CN 201510697559A CN 105145641 B CN105145641 B CN 105145641B
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Abstract
The present invention relates to a kind of granule containing trichoderma harzianum and amino-oligosaccharide, the active component contained is amino-oligosaccharide, trichoderma harzianum, humic acid and zinc sulfate.Described trichoderma harzianum is set as 100% female medicine by 30,000,000,000 CFU/ grams of female medicines of content, and the weight ratio of amino-oligosaccharide, trichoderma harzianum and humic acid is 3 25:1‑2:30‑90.The present invention is prepared into granule, and medicament is easy to use, and being advantageous to germ, occurrence in peak period can be prevented and treated timely after the rain, reduces loss.Active component amino-oligosaccharide, trichoderma harzianum and humic acid have Synergistic function in antibacterial, and rational ratio mixture can reduce the usage amount of each single dose, help to save drug cost.The present invention can effectively supplement zinc fertilizer, zinc is favorably improved the rhizome resistance against diseases of crop, promotes root healthy and strong, improves crop anti-adversity containing zinc microelement fertilizer while controlling disease.
Description
Technical field
The present invention relates to cultural control technical field, specifically a kind of particle containing trichoderma harzianum and amino-oligosaccharide
Agent.
Background technology
Soil-borne disease refers to that pathogen such as fungi, bacterium, nematode and virus are lived in soil with invalid body, and condition is suitable
When from crop root or stem infringement crop caused by disease.It is soil that soil continuous cropping, multiple crop index, which improve and applied fertilizer and be unreasonable,
Pass the increasing reason of disease.Soil-borne disease often result in crops seedling stage damping off, root-rot or vertical withered, the disconnected ridge that is short of seedling is caused, if hair
It is sick serious, the whole dead consequences of seedbed nursery can be caused.
The pathogen of soil-borne disease is just already present in soil before crop-planting, and people do not form medication also at present
Prevent the custom of soil-borne disease, usually disease is just watered medication spraying preventing and treating after occurring, if running into the big wet weather of soil moisture
The morbidity of weather disease is heavier, be watered spray it is cumbersome, and be watered spray do not meet peasant often in soil or crop leaf compared with
Medication in the case of dry, therefore disease cannot be prevented and treated timely, cause the massive losses of part death of seedling.
In view of problem above, is prepared into granule, the soil after crop sowing time, transplanting time or rain by soil-borne disease medicament
High humidity is administered, and is the main path for solving above-mentioned technical problem.
Trichoderma harzianum, it is the microbial bactericide produced by Bai Wo limited companies of the U.S., is mainly used in preventing and treating and plants
The fungal disease of thing root.Trichoderma harzianum can not only effectively suppress the growth of germ, and can produce stimulate plant growth and
The compound of plant defense response is induced, improves the microenvironment of root system, strengthens the growing way and resistance against diseases of plant, improve crop
Yield and income.
Amino-oligosaccharide, chemical name：Oligomeric-D Glucosamines, one kind of medicine microorganism belonging to genus metabolism extraction, which has, to be resisted
The bactericide of disease effect, by inducing plant to produce disease resistance, suppresses the gene expression of germ, to a variety of fungies, bacterium and disease
Poison produces immune and killing action.Amino-oligosaccharide can also stimulating growth, improve the kind and yield of crop.Amino-oligosaccharide
Applied to the existing relevant report of preventing and treating soil-borne disease, as Yao Manchang, Zhang Mingzhi et al. are delivered《Amino-oligosaccharide is withered to watermelon
The controlling experiment of disease》Point out that amino-oligosaccharide has preferable prevention effect to watermelon blight, while it can improve plant strain growth
Environment, promotion plant strain growth can be made, make robust plant, well developed root system, reach volume increase, improve the effect of quality.
Humic acid is a kind of natural organic high molecular compound, complicated, have ion exchange, chelating, flocculation and
The functions such as colloidal sol, agriculturally there is improved soil, stimulating growth, strengthening degeneration-resistant, improving quality.Humic acid is applied to
The existing relevant report of soil-borne disease, such as highlighted deliver《Humic acid biological and ecological methods to prevent plant disease, pests, and erosion biological organic fertilizer is to Fusarium solani prevention effect
Research》Point out, the organic fertilizer containing humic acid and biocontrol microorganisms can effectively reduce the generation and harm of Fusarium solani, improve preventing and treating effect
Fruit.
In agricultural production, disease control is carried out with single medicament for a long time, germ easily produces resistance so that prevention effect
It is undesirable.At present, the granule using amino-oligosaccharide, trichoderma harzianum and humic acid as active component is also without the report of correlation
Road.
The content of the invention
It is an object of the present invention to provide a kind of suitable disease period of disease to carry out medication preventing and treating, meets farming operations custom,
And be advantageous to delay the granule containing trichoderma harzianum and amino-oligosaccharide of germ resistance.
The technical solution adopted in the present invention is：A kind of granule containing trichoderma harzianum and amino-oligosaccharide, contains
Active component is amino-oligosaccharide, trichoderma harzianum, humic acid and zinc sulfate.
Described active component, trichoderma harzianum are set as 100% female medicine, ammonia by 30,000,000,000 CFU/ grams of female medicines of content
The weight ratio of base oligosaccharide, trichoderma harzianum and humic acid is 3-25:1-2:30-90, preferably 3-15:1-2:30-50
Described active component, percentage by weight of the amino-oligosaccharide in granule are 0.1%-1.5%, Trichoderma harzianum
The percentage by weight of bacterium in the composition is 0.1%-0.5%, and humic acid percentage by weight in granule is 2.0-8.0%,
Percentage by weight of the zinc sulfate in granule is 3.0%-5.0%.
The adjuvant that the described granule containing amino-oligosaccharide and trichoderma harzianum uses includes filler, binding agent, divided
Powder, wetting agent, disintegrant, sustained release agent, stabilizer or it is other be beneficial to active ingredient in the formulation stablize and drug effect play
Material, all it is to commonly use in agriculture field preparation or allow the various composition used, is not particularly limited, specific composition and dosage root
Determined according to recipe requirements by simple experiment.
The present invention has advantages below and significant technique effect:
(1) be prepared into granule, be accustomed to reference to farming operations, be advantageous to that soil moisture is big and rain after become a fine day peasant and easily connect
The characteristics of dispenser being carried out by granule.
(2) medicament is easy to use, by spreading fertilizer over the fields, spreading manuer in holes or ditch spread, without spraying is watered, overcomes agriculture after soil relatively dry
The people just carry out being watered the hysteresis preventing and treating of spray, and being advantageous to germ, occurrence in peak period can be prevented and treated timely after the rain, reduce damage
Lose.
(3) active component amino-oligosaccharide, trichoderma harzianum and humic acid have Synergistic function in antibacterial, reasonably
Ratio mixture can reduce the usage amount of each single dose, help to save drug cost
(4) present invention can effectively supplement zinc fertilizer, zinc helps to carry containing zinc microelement fertilizer while controlling disease
The rhizome resistance against diseases of high crop, promote root healthy and strong, improve crop anti-adversity.
Embodiment
Technical scheme and technique effect are described further with reference to specific embodiment, institute of the present invention
The percentage stated is weight percentage.
Embodiment 1：Amino-oligosaccharide, trichoderma harzianum, humic acid and mixed agent are surveyed to the indoor virulence of watermelon blight
It is fixed
Subjects：Watermelon blight (Fusarium oxysporum f.sp.niveum.), is gathered by field, through room
Inside isolate and purify.
Test method：After prerun determines the effective inhibition concentration scope of each medicament, by amino-oligosaccharide, trichoderma harzianum,
Humic acid and its mixture preparation dilute 5 concentration by active constituent content gradient series, are compared with sterilized water.Reference《China
People's republic agricultural industry criteria NY/T1156.2-2006》, determined using mycelial growth rate method.Aseptically, take
The decoction 5mL configured is well mixed with 75mL culture mediums, and 4 drug containing flat boards are made, are mixed with equivalent sterilized water with culture medium
As control.Cultured withered germ of water-melon is aseptically cut into bacterium with diameter 5mm sterilization punchers from edge
Cake.With inoculator by pure culture biscuits involvng inoculation in drug containing flat board center, mycelia is face-down, is placed in 25 DEG C of incubators.Given birth to depending on blank control
Long condition survey result, each bacterium colony is each once with crossing method vertical survey diameter, takes its average value, calculates mycelial growth
Inhibiting rate, calculate EC50, obtain toxicity regression formula.Mycelial growth inhibition rate formula is as follows：
Mycelial growth inhibition rate (%)=(blank control bacterium colony increases diameter-chemicals treatment bacterium colony and increases diameter)/blank
Compare bacterium colony and increase diameter × 100
The synergistic effect of pharmacy mix is evaluated with the co-toxicity coefficient method (CTC) of the abundant methods of Sun Yun, i.e., CTC≤80 are that antagonism is made
With 80<CTC<120 be summation action, and CTC >=120 are synergistic effect.
Toxicity test the results are shown in Table 1.
The Toxicity Determination table of the amino-oligosaccharide of table 1, trichoderma harzianum, humic acid and mixed agent to watermelon blight
As shown in Table 1, the EC of amino-oligosaccharide, trichoderma harzianum and humic acid to withered germ of water-melon50Respectively
35.30mg/L, 5.01mg/L and 105.83mg/L, when the mixed ratio of amino-oligosaccharide, trichoderma harzianum and humic acid is in 3-
25:1-2:During 30-90, co-toxicity coefficient (CTC) is all higher than 120, there is good synergistic function.As amino-oligosaccharide, Ha Ci
The mixed ratio of Trichoderma and humic acid is 15:2:30, co-toxicity coefficient (CTC) is 258.6, and synergistic effect is most obvious.Work as amino
The weight ratio of oligosaccharide, trichoderma harzianum and humic acid is 1:5：When 20, co-toxicity coefficient (CTC) is 53.4, amino-oligosaccharide, Kazakhstan
Mixture produces antagonism between thatch Trichoderma and humic acid.
Embodiment 2：The cfu/ grams of humic acid granule of trichoderma harzianum 5% of 0.3% amino-oligosaccharide 0.6 hundred million
Weight ratio according to amino-oligosaccharide, trichoderma harzianum and humic acid is 3:2:When 50, composition has synergy work
With weighing according to quantity, 80% amino-oligosaccharide mother's medicine 0.375kg, 30,000,000,000 cfu/ grams of Trichoderma harzianum starter medicine 0.2kg, humic acid
5kg, zinc sulfate 3kg, naphthalenesulfonate formaldehyde condensation compound 2.5kg, lauryl sodium sulfate 1.5kg, polyvinyl alcohol 2.5kg are concavo-convex
Rod soil 5kg, bentonite complement to 100kg.
Above-mentioned recipe ingredient is well mixed, crushed, adds water to be stirred after soaking, then use screw extruder pelletizer
It is granulated, is sieved after drying and produce the cfu/ grams of humic acid granule of trichoderma harzianum 5% of 0.3% amino-oligosaccharide 0.6 hundred million.
By formulation application prepared by embodiment 2 in the field experiment of preventing and treating rice seedling blight
(1) experimental design
Using the preparation that embodiment 2 is prepared into as test group 1#, amount of application is 0.25 kilogram/m2；Respectively with 2.5% amino
Oligosaccharide granule 1# as a control group, 1.0 hundred million cfu/ grams of trichoderma harzianum granules 2# as a control group, 10% humic acid
3#, amount of application are respectively 0.5 kilogram/m to granula as a control group2, 1 blank control group is set.
(2) application method
Disease-free seedling plot is selected, levels land into seedling furrow, the soil that damping-off is seriously occurred is pulverized and mixed
Even, being layered on will test on seedling furrow successively, it is desired to which the thickness of soil in spite of illness on furrow is consistent.By the seed rice for having urged bud plant into
In soil, each 3 repetitions of processing, plot area 0.5m in experiment2, by experimental design by experimental group 1#, control group 1#, control
Group 2#, control group 3# medicament are uniformly spread fertilizer over the fields in corresponding cell, and holding seedbed environment is consistent in each cell.
(3) result of the test
Each cell is investigated in disease peak period, calculates the incidence of disease and prevention effect.Statistical analysis, 20 days after dispenser, disease
Evil peak period investigation result, experimental group 1# prevention effect is 88.76%, and control group 1# prevention effect is 65.24%；Control
Group 2# prevention effect is 72.52%；Control group 3# prevention effect is 54.21%.Find out from experiment, amino-oligosaccharide,
After trichoderma harzianum carries out rational mixture with humic acid, synergy of the mixed agent to rice seedling blight is obvious, both reduces active ingredient
Usage amount, show the field efficacy better than single dose again.
Embodiment 3：The cfu/ grams of humic acid granule of trichoderma harzianum 3% of 0.2% amino-oligosaccharide 0.1 hundred million
Weight ratio according to amino-oligosaccharide, trichoderma harzianum and humic acid is 6：1:When 90, composition has synergy work
With weighing according to quantity, 80% amino-oligosaccharide mother's medicine 0.75kg, 30,000,000,000 cfu/ grams of Trichoderma harzianum starter medicine 0.1kg, humic acid
9.0kg, zinc sulfate 12kg, AEO 7.5kg, lauryl sodium sulfate 7.5kg, carboxymethyl cellulose 6.0kg,
Diatomite 45kg, bentonite complement to 300kg.
Above-mentioned recipe ingredient is well mixed, crushed, adds water to be stirred after soaking, then use screw extruder pelletizer
It is granulated, is sieved after drying and produce the cfu/ grams of humic acid granule of trichoderma harzianum 3% of 0.2% amino-oligosaccharide 0.1 hundred million.
By the formulation application that embodiment 3 is prepared into the field medicine effect test of preventing and treating cucumber rhizoctonia rot
(1) experimental design
Using the preparation that embodiment 3 is prepared into as test group 1#, amount of application is 15 kgs/acre；It is few with 1.5% amino respectively
Sugared crude granule agent 1# as a control group, 1.0 hundred million cfu/ grams of trichoderma harzianum granules 2# as a control group, 10% humic granulates
3#, amount of application are respectively 30 kgs/acre as a control group for agent, set 1 blank control group.
(2) application method
Select continuous cropping cucumber rhizoctonia rot that serious plot occurs, by experimental design by experimental group 1#, control group 1#, control group
2#, control group 3# medicament uniformly mix soil in sowing cave, are planted afterwards in cave is sowed into the cucumber seeds for having urged bud, per cave 3
Cucumber seedling, it is each to handle 100 caves, keep that soil moisture is consistent, and other farming operations are normally carried out in each processing.
(3) investigation method
20 days disease onset peak period statistics incidences, calculate prevention effect and the state of an illness are divided into 5 grades after dispenser：0 grade
There is no Disease symptoms for plant；1 grade is that plant radical leaves slight withered and yellow symptom occur, and slight brown stain occurs in root system；2 grades are plant
There is withered and yellow symptom in strain middle and lower part, and brown stain occurs in root system, but terminal bud is good；3 grades are that complete stool withered and yellow symptom occurs, and root system brown stain is rotten
It is rotten；4 grades are plant withered death, and the serious brown stain of root system is rotted.
Diseased plant rate (%)=diseased plant number/investigation total strain number × 100%
Disease index=∑ (morbidity number × levels at different levels represent number)/(sum × highest typical value) × 100
Prevention effect (%)=(control disease index-processing disease index)/control disease index × 100%
(4) result of the test
Calculated through statistics, test group 1# prevention effect is 86.35%；Control group 1# prevention effect 64.27% compares
Group 2# prevention effect 68.25%；Control group 3# prevention effect 58.54%.
From field medicine effect test, it can be seen that synergy is obvious after amino-oligosaccharide, trichoderma harzianum and humic acid compounding,
Both the usage amount of active ingredient had been reduced, has shown the field efficacy better than single dose again.
Claims (3)
1. a kind of granule containing trichoderma harzianum and amino-oligosaccharide, it is characterised in that by amino-oligosaccharide, Trichoderma harzianum
Bacterium, humic acid, zinc sulfate and it is prepared into the adjuvant of granule and is prepared, described trichoderma harzianum is using content as 300
Hundred million CFU/ grams of female medicines are set as 100% female medicine, and the weight ratio of amino-oligosaccharide, trichoderma harzianum and humic acid is 3-25:1-
2:30-90。
2. the granule according to claim 1 containing trichoderma harzianum and amino-oligosaccharide, it is characterised in that amino-oligosacchride
The weight ratio of element, trichoderma harzianum and humic acid is 3-15:1-2:30-50.
3. the granule according to claim 1 or 2 containing trichoderma harzianum and amino-oligosaccharide, it is characterised in that amino
Percentage by weight of the oligosaccharide in granule is 0.1%-1.5%, and the percentage by weight of trichoderma harzianum in the composition is
0.1%-0.5%, humic acid percentage by weight in granule are 2.0-8.0%, weight percent of the zinc sulfate in granule
Than for 3.0%-5.0%.
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| CN105766900A (en) * | 2016-04-01 | 2016-07-20 | 广西南宁益土生物科技有限责任公司 | Nematode granula for promoting slow seedling of crops |
| CN106386869A (en) * | 2016-08-31 | 2017-02-15 | 上海交通大学 | Trichoderma seed coating as well as preparation method and application thereof |
| CN107969438B (en) * | 2017-12-19 | 2021-04-06 | 北京科威拜沃生物技术有限公司 | Wettable powder containing trichoderma harzianum T-22 and copper ammine, and preparation method and application thereof |
| CN113396936A (en) * | 2021-04-26 | 2021-09-17 | 海南金雨丰生物工程有限公司 | Disease-preventing and insect-preventing composition and preparation method and application thereof |
| CN114088824B (en) * | 2021-10-15 | 2023-08-08 | 陈炜瑶 | Method for detecting free glucosamine in amino-oligosaccharin pesticide |
| CN115152773B (en) * | 2022-06-17 | 2023-12-29 | 安徽久易农业股份有限公司 | Composition of prothioconazole and fludioxonil for preventing and treating wheat stem rot |
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