CN110622982A - Sterilization composition containing zhongshengmycin - Google Patents
Sterilization composition containing zhongshengmycin Download PDFInfo
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- CN110622982A CN110622982A CN201910776311.XA CN201910776311A CN110622982A CN 110622982 A CN110622982 A CN 110622982A CN 201910776311 A CN201910776311 A CN 201910776311A CN 110622982 A CN110622982 A CN 110622982A
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- zhongshengmycin
- hydrazide
- chloroindole
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
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- General Health & Medical Sciences (AREA)
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Abstract
The invention belongs to the field of pesticides, and provides a bactericidal composition containing zhongshengmycin, which comprises the main active ingredients of chloroindole hydrazide and zhongshengmycin, wherein the weight ratio of the chloroindole hydrazide to the zhongshengmycin is 0.5: 5-40: the content of the chloroindoxyl in the compound preparation is 0.5-40%, the content of the zhongshengmycin in the compound preparation is 0.5-10%, the composition can be used for various virus diseases of vegetables, melons and fruits, crops and the like, particularly common cucumber bacterial angular leaf spot, Chinese cabbage soft rot, potato virus Y, camellia mosaic virus and peanut dwarf virus, has good effects on tomato yellow leaf curl virus diseases, rice black streaked dwarf disease and the like which are difficult to prevent and treat, has prevention and treatment effects, prevents and controls the occurrence and development of plant virus diseases, can achieve remarkable prevention and treatment effects before or after the occurrence of the diseases, and reduces the dosage of pesticides.
Description
Technical Field
The invention belongs to the technical field of pesticides, and provides a sterilization composition, in particular to a sterilization composition containing zhongshengmycin.
Background
The compound containing the acylhydrazone structure is a pesticide active ingredient developed by southern Kao university, wherein the chemical name of the active compound is (1S, 3S) -N' - (4-chlorphenyl methylene) -1-methyl-2, 3,4, 9-tetrahydropyrido [3,4-b ] indole-3-formylhydrazine), which is called chloroindole hydrazide for short. The compound containing the acylhydrazone structure has very good biological activity on tobacco mosaic virus, tomato mosaic virus and the like, and meanwhile, the compound is easy to be absorbed by plants, easy to degrade in the environment and very good in environmental compatibility.
The compound chloroindole hydrazide has the following structure:
zhongshengmycin is an N-glycoside antibiotic, and ultimately causes bacterial death by inhibiting peptide bond formation of pathogenic bacterial proteins. It can inhibit the growth of fungus hypha and spore germination, prevent and control fungous diseases, stimulate the generation of plant protecting matter and lignin precursor in plant body, raise the disease resistance of plant, and make the zhongshengmycin resist gram-positive and gram-negative bacteria, mycobacterium, saccharomycete and fungus, especially crop pathogenic bacteria including soft rot of cabbage, cucumber angular leaf spot, rice leaf blight, apple ring rot, wheat scab, etc.
Bacterial angular leaf spot is a common bacterial disease of cucumbers, and bacterial pus is often overflowed from diseased spots on the backs of diseased leaves, and the cucumber strips are damaged by the bacteria and have odor. The soft rot of Chinese cabbage is a common bacterial disease of Chinese cabbage, and the stem base of the diseased plant is often soft and rotten and emits foul smell. The agricultural drug for preventing and controlling the bacterial diseases has several fingers, and the resistance of pathogenic bacteria is caused by using the same drug all year round.
The compounding of different pesticide varieties is a common method for preventing and treating agricultural resistant diseases. How to obtain a better bactericidal composition by using the chloroindole hydrazide containing the acylhydrazone structure becomes one of the problems to be solved in the prior art.
Disclosure of Invention
Aiming at the blank in the prior art, the invention provides a bactericidal composition containing zhongshengmycin, the main active ingredients of the composition are chloroindole hydrazide and zhongshengmycin, wherein the weight ratio of the chloroindole hydrazide to the zhongshengmycin is 0.5: 5-40: 1, the content of the chloroindoxyl in the compound preparation is 0.5-40 wt%, the content of the zhongshengmycin compound preparation is 0.5-10 wt%, the composition can be used for various virus diseases of vegetables, melons and fruits, crops and the like, particularly common cucumber bacterial angular leaf spot, cabbage soft rot, potato virus Y, camellia mosaic virus and peanut dwarf virus, has good effects on tomato yellow leaf curl virus diseases, rice black streaked dwarf disease and the like which are difficult to control, has prevention and treatment effects, prevents and controls the occurrence and development of plant virus diseases, can achieve remarkable prevention and treatment effects before or after the occurrence of the diseases, and reduces the dosage of pesticides.
The inventor of the invention has conducted intensive research on screening of a compound formula of zhongshengmycin and chloroindoxyl based on a large number of indoor toxicity and field efficacy tests, finds that compounding of zhongshengmycin and chloroindoxyl has an obvious synergistic effect within a certain range of proportion, and has excellent control effects on cucumber bacterial angular leaf spot and cabbage soft rot, and further researches complete the invention.
The specific technical scheme of the invention is as follows:
the bactericidal composition containing zhongshengmycin comprises the main active ingredients of chloroindole hydrazide and zhongshengmycin, wherein the weight ratio of the chloroindole hydrazide to the zhongshengmycin is 0.5: 5-40: 1, wherein the content of the chloroindoyl hydrazide in the compound preparation is 0.5-40 wt%, and the content of the zhongshengmycin compound preparation is 0.5-10 wt%;
wherein the chloroindole hydrazide is (1S, 3S) -N' - (4-chlorphenyl methylene) -1-methyl-2, 3,4, 9-tetrahydropyrido [3,4-b ] indole-3-formylhydrazine;
after the content of the main active components is determined, the inventor further prepares the composition into various dosage forms, mainly including suspending agents, wettable powder, water dispersible granules, aqueous emulsion, microemulsion and other dosage forms, the specific preparation method is a conventional preparation method in the field, the selected auxiliary materials and fillers comprise one or a mixture of more of emulsifying agents, wetting agents, stabilizing agents, dispersing agents, thickening agents, defoaming agents, antifreezing agents, disintegrating agents, binding agents, filling agents, media and the like, the auxiliary materials and the fillers are known substances and are various commonly used auxiliary agents in pesticide preparations, and the auxiliary materials and the fillers can be changed according to different conditions, and the inventor does not need to describe any more herein;
furthermore, the weight ratio of the chloroindole hydrazide to the zhongshengmycin is 20:1-5:1, and the synergistic effect of the chloroindole hydrazide and the zhongshengmycin is obvious in the range;
the bactericidal composition provided by the inventor is mainly used for preventing and treating agricultural bacterial diseases, particularly has the best prevention and treatment effects on cucumber bacterial angular leaf spot and Chinese cabbage soft rot, can be used by adopting a spraying method during prevention and treatment, and can also adopt other application technologies applied in agriculture according to needs;
compared with the prior art, the pesticide composition provided by the invention has the following beneficial effects:
1. the compounding of the zhongshengmycin and the chloroindole hydrazide has obvious synergistic effect, improves the control effect of diseases compared with the single agent, fills the blank of the field, and belongs to the first application of the field;
2. the action mechanisms of the zhongshengmycin and the chloroindole hydrazide are different from each other, so that the generation of the drug resistance of diseases can be delayed; the chloroindole hydrazide has certain systemic activity, the zhongshengmycin has contact killing and permeation effects, and two medicaments with different action mechanisms are subjected to binary compounding, so that the advantages can be complemented, the problem of drug resistance caused by the use of a single medicament can be effectively delayed, the service life of the medicament can be prolonged, and the chloroindole hydrazide has important significance for the comprehensive control of agricultural harmful bacteria;
3. the two effective components of zhongshengmycin and chloroindole hydrazide are compounded, so that the use amount of pesticides is reduced, the use cost is reduced, and the pollution to the environment is reduced.
Detailed Description
The present invention will be described in further detail with reference to the following examples, but it should not be construed that the scope of the above subject matter is limited to the following examples. All the technologies realized based on the above contents of the invention belong to the scope of the invention, the following percentages are weight percentages, and the adopted auxiliary agents are all conventional auxiliary agents in the field.
Example 1: 41% chloroindoxyl hydrazide and zhongshengmycin water dispersible granule
Weighing 40% of chloroindole hydrazide, 1% of zhongshengmycin, 5% of mineral oil, 5% of nonylphenol polyoxyethylene ether, 5% of osmotic agent fast T, 2% of sodium dodecyl sulfate, 5% of sodium lignin sulfonate and 4% of naphthalene sulfonate, and supplementing bentonite to 100%. The raw materials are mixed, subjected to superfine jet milling and granulated to prepare 41% of zhongshengmycin and chloroindole hydrazide water dispersible granules.
Example 2: 31.5% chloroindole hydrazide and zhongshengmycin wettable powder
Weighing 30% of chloroindole hydrazide, 1.5% of zhongshengmycin, 5% of nekal, 5% of naphthalenesulfonate formaldehyde condensate, 10% of talcum powder and kaolin to make up 100%. The raw materials are mixed and pulverized by air flow to prepare 31.5 percent of the wettable powder of the zhongshengmycin and the chloroindole hydrazide.
Example 3: 22% chloroindole hydrazide + zhongshengmycin hydrazine suspension
Weighing 20% of chloroindoxyl hydrazide, 2% of zhongshengmycin, 5% of sodium lignosulfonate, 2.0% of OP-10, 1.5% of CMC (carboxymethyl cellulose), 0.02% of organic silicon, 2% of glycerol and deionized water to make up 100%. The raw materials are mixed, sheared and dispersed at a high speed for 30min, and ground by a sand mill to prepare the 22% chloroindole hydrazide and zhongshengmycin suspending agent.
Example 4: 24% chloroindole hydrazide + zhongshengmycin suspension
Weighing 20% of chloroindoxyl hydrazide, 4% of zhongshengmycin, 8% of sodium lignosulfonate, 2.5% of OP-10, 1% of CMC (carboxymethyl cellulose), 0.02% of organic silicon, 2% of glycerol and deionized water to make up 100%. The raw materials are mixed, sheared and dispersed at a high speed for 30min, and then ground by a sand mill to prepare the 24% chloroindole hydrazide and zhongshengmycin suspending agent.
Example 5: microemulsion of 10% chloroindole hydrazide and zhongshengmycin
Weighing 5% of chloroindoxyl hydrazide, 5% of zhongshengmycin, 10% and 2% of phenethylphenol polyoxyethylene ether, 500# of agricultural milk, 5% of isopropanol, 10% of xylene and 7% of N, N-dimethylformamide, fully stirring and dissolving, supplementing the deionized water to 100%, and continuously stirring until the mixture is transparent to prepare the 10% chloroindoxyl hydrazide and zhongshengmycin microemulsion.
Example 6: microemulsion of 5.5% chloroindole hydrazide and zhongshengmycin
Weighing 0.5% chloroindoxyl hydrazide, 5% zhongshengmycin, 15% phenethylphenol polyoxyethylene ether, 500# Nongru, 5% isopropanol, 10% xylene and 10% N, N-dimethylformamide, fully stirring and dissolving, supplementing the deionized water to 100%, and continuously stirring until the mixture is transparent to prepare the 5.5% zhongshengmycin + chloroindoxyl microemulsion.
Experimental example 1 indoor combined virulence determination for cucumber bacterial angular leaf spot
The test medicament adopts 95 percent chloroindole hydrazide and 98 percent zhongshengmycin original drug.
The insecticidal activity of each agent was evaluated by performing statistical analysis using the DPS statistical analysis software to calculate LC50 for each agent. And calculating the co-toxicity coefficient (CTC value) of the mixture according to the Sun Yunpei method.
The combination was found to have a virulence index (ATI) ═ 100 x (standard agent LC 50/test agent LC 50).
The Theoretical Toxicity Index (TTI) of the mixed preparation is A medicament toxicity index multiplied by the percentage of A in the mixed preparation, B medicament toxicity index multiplied by the percentage of B in the mixed preparation.
Co-toxicity coefficient (CTC) × 100 [ measured toxicity index (ATI) of the mixture)/Theoretical Toxicity Index (TTI) of the mixture ].
The synergistic effect evaluation method refers to the bioassay standard method NY/T1156.6-2006, and the synergistic effect of the drug mixture is evaluated according to the co-toxicity coefficient method (CTC) of Sun & Johnson (1960): the co-toxicity coefficient (CTC) is more than or equal to 120 and shows a synergistic effect; the co-toxicity coefficient (CTC) is less than or equal to 80, and the antagonism is shown; the 80 < co-toxicity coefficient (CTC) < 120 shows additive effect.
The results are shown in table 1:
TABLE 1 indoor combined virulence determination results for cucumber bacterial angular leaf spot pathogen
As can be seen from Table 1, the compositions of examples 1-6 of the present invention have high activity against cucumber bacterial angular leaf spot germs, and particularly, the mass ratio of the chloroindoxyl to the zhongshengmycin is 20:1-5: within the range of 1, the synergistic effect is more obvious, the co-toxicity coefficient (CTC) is more than 140, and the compounding of the chloroindole hydrazide and the zhongshengmycin is reasonable and feasible.
Experimental example 2 field efficacy test for cucumber bacterial angular leaf spot
The test site is located in a certain cucumber garden in Shouguang city, Shandong province. The pesticide is applied at the early stage of the occurrence of the cucumber bacterial angular leaf spot by adopting a spraying method, 5 percent of zhongshengmycin wettable powder and 30 percent of indoxyl hydrazide wettable powder are used as control reagents, 8 pesticide treatments are set in the test, and each treatment is repeated for 3 times. The number of plants was examined at five diagonal points in each treatment area before application, and 20 cucumbers were examined at each point. The control effect was investigated 3 days, 7 days and 14 days after application, the number of plants with disease was recorded, and the control effect was calculated, and the test results are shown in table 2.
Table 2, test results of zhongshengmycin and chloroindoxyl mixture for preventing and treating cucumber angular leaf spot
From the table 2, the control effect of the compound preparation of the zhongshengmycin and the chloroindoxyl on the cucumber bacterial angular leaf spot is obviously higher than that of 5 percent zhongshengmycin wettable powder and 30 percent chloroindoxyl wettable powder in the same period after 3d, 7d and 14d, which indicates that the compound preparation of the zhongshengmycin and the chloroindoxyl can improve the control effect on the cucumber bacterial angular leaf spot compared with the single use of two single preparations, and meanwhile, the control effect of 14d after the compound preparation is used is still more than 80 percent, which indicates that the compound preparation has good persistence, and the compound preparation is not found to cause phytotoxicity on cucumbers during the test period.
In conclusion, compared with the existing single preparation, the bactericidal composition provided by the invention not only has an obvious synergistic effect, but also has a good bactericidal effect, is long in lasting period, safe for crops, and capable of reducing the dosage, and is worthy of popularization and application in agricultural production.
Claims (4)
1. The bactericidal composition containing the zhongshengmycin is characterized in that: the main active ingredients are chloroindole hydrazide and zhongshengmycin, wherein the chloroindole hydrazide is (1S, 3S) -N' - (4-chlorphenyl methylene) -1-methyl-2, 3,4, 9-tetrahydropyrido [3,4-b ] indole-3-formylhydrazine; the weight ratio of the chloroindole hydrazide to the zhongshengmycin is 0.5: 5-40: 1, the content of the chloroindole hydrazide in the compound preparation is 0.5-40 wt%, and the content of the zhongshengmycin in the compound preparation is 0.5-10 wt%.
2. The zhongshengmycin-containing bactericidal composition according to claim 1, wherein:
the weight ratio of the chloroindole hydrazide to the zhongshengmycin is 20:1-5: 1.
3. The zhongshengmycin-containing bactericidal composition according to claim 1, wherein:
the formulation of the bactericidal composition containing the zhongshengmycin is one of a suspending agent, wettable powder, water dispersible granules, an aqueous emulsion and a microemulsion.
4. The method of using the zhongshengmycin-containing bactericidal composition of claim 1, wherein the method comprises the steps of: when in use, the active component is diluted by water until the concentration of the active component is 100-200 ppm.
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| CN201910776311.XA CN110622982A (en) | 2019-08-21 | 2019-08-21 | Sterilization composition containing zhongshengmycin |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111766313A (en) * | 2020-06-04 | 2020-10-13 | 京博农化科技有限公司 | Chloroindole hydrazide content analysis method |
| CN112021326A (en) * | 2020-09-08 | 2020-12-04 | 京博农化科技有限公司 | Bactericidal composition containing chlorine indole hydrazide and agricultural streptomycin and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106565589A (en) * | 2016-10-13 | 2017-04-19 | 云南大学 | Preparation method and application of hydrazide indoles drugs |
| CN109757497A (en) * | 2018-12-28 | 2019-05-17 | 京博农化科技有限公司 | A kind of bactericidal composition |
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2019
- 2019-08-21 CN CN201910776311.XA patent/CN110622982A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106565589A (en) * | 2016-10-13 | 2017-04-19 | 云南大学 | Preparation method and application of hydrazide indoles drugs |
| CN109757497A (en) * | 2018-12-28 | 2019-05-17 | 京博农化科技有限公司 | A kind of bactericidal composition |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111766313A (en) * | 2020-06-04 | 2020-10-13 | 京博农化科技有限公司 | Chloroindole hydrazide content analysis method |
| CN111766313B (en) * | 2020-06-04 | 2022-07-01 | 京博农化科技有限公司 | Chloroindole hydrazide content analysis method |
| CN112021326A (en) * | 2020-09-08 | 2020-12-04 | 京博农化科技有限公司 | Bactericidal composition containing chlorine indole hydrazide and agricultural streptomycin and application thereof |
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Application publication date: 20191231 |