ZA202101476B - Methods and compositions for nucleic acid isolation - Google Patents
Methods and compositions for nucleic acid isolationInfo
- Publication number
- ZA202101476B ZA202101476B ZA2021/01476A ZA202101476A ZA202101476B ZA 202101476 B ZA202101476 B ZA 202101476B ZA 2021/01476 A ZA2021/01476 A ZA 2021/01476A ZA 202101476 A ZA202101476 A ZA 202101476A ZA 202101476 B ZA202101476 B ZA 202101476B
- Authority
- ZA
- South Africa
- Prior art keywords
- compositions
- methods
- nucleic acid
- acid isolation
- isolation
- Prior art date
Links
- 238000002955 isolation Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 239000000203 mixture Substances 0.000 title abstract 2
- 150000007523 nucleic acids Chemical class 0.000 title abstract 2
- 102000039446 nucleic acids Human genes 0.000 title abstract 2
- 108020004707 nucleic acids Proteins 0.000 title abstract 2
- 239000012472 biological sample Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0045—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Galacturonans, e.g. methyl ester of (alpha-1,4)-linked D-galacturonic acid units, i.e. pectin, or hydrolysis product of methyl ester of alpha-1,4-linked D-galacturonic acid units, i.e. pectinic acid; Derivatives thereof
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862765013P | 2018-08-17 | 2018-08-17 | |
| PCT/US2019/046908 WO2020037260A1 (en) | 2018-08-17 | 2019-08-16 | Methods and compositions for nucleic acid isolation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ZA202101476B true ZA202101476B (en) | 2024-08-28 |
Family
ID=67809720
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ZA2021/01476A ZA202101476B (en) | 2018-08-17 | 2021-03-03 | Methods and compositions for nucleic acid isolation |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US12365891B2 (https=) |
| EP (1) | EP3837363A1 (https=) |
| JP (1) | JP7516348B2 (https=) |
| KR (1) | KR102900265B1 (https=) |
| CN (1) | CN113195714A (https=) |
| AU (1) | AU2019320825B2 (https=) |
| CA (1) | CA3109805A1 (https=) |
| WO (1) | WO2020037260A1 (https=) |
| ZA (1) | ZA202101476B (https=) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113281503B (zh) * | 2020-10-29 | 2022-01-04 | 杭州微策生物技术股份有限公司 | 一种用于裂解病毒样本的试剂 |
| CN115197935B (zh) * | 2021-04-08 | 2025-10-21 | 上海细胞治疗集团股份有限公司 | 纤维素色谱纯化rna的方法 |
| CN114807308B (zh) * | 2022-05-07 | 2025-10-10 | 英科新创(苏州)生物科技有限公司 | 核酸释放剂、试剂或试剂盒、其应用及核酸检测方法 |
| CN115873664B (zh) * | 2022-12-06 | 2024-08-13 | 上海领骏生物科技有限公司 | 一种用于核酸提取磁珠的清洗液及其使用方法 |
| WO2025101548A2 (en) | 2023-11-08 | 2025-05-15 | Cepheid | Her2 low quantification method |
| WO2026055639A1 (en) | 2024-09-06 | 2026-03-12 | Cepheid | Sample in sequencing-ready library out automated library preparation solution |
Family Cites Families (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1264321B (it) | 1992-01-13 | 1996-09-23 | Mini Ricerca Scient Tecnolog | Esteri di acidi pectici e pectinici, procedimento di preparazione e loro applicazioni farmaceutiche e biosanitarie |
| AU682226B2 (en) | 1993-11-23 | 1997-09-25 | Ciba Corning Diagnostics Corp. | Use of antisense oligomers in a process for controlling contamination in nucleic acid amplification reactions |
| GB9927904D0 (en) | 1999-11-25 | 2000-01-26 | Amersham Pharm Biotech Ab | A method fro obtaining a nucleic acid variant |
| US6374684B1 (en) | 2000-08-25 | 2002-04-23 | Cepheid | Fluid control and processing system |
| EP1390514A2 (en) | 2001-05-04 | 2004-02-25 | Ato B.V. | Regulatory protein involved in pectin modification |
| WO2003000776A1 (en) * | 2001-05-14 | 2003-01-03 | Johns Hopkins Singapore Pte Ltd | Biodegradable polyphosphoramidates for controlled release of bioactive substances |
| DK176653B1 (da) | 2002-07-02 | 2009-02-02 | Cp Kelco Aps | Fremgangsmåde til fremstilling af amideret pektin med lav forestringsgrad, amideret pektin, som er opnåeligt ved fremgangsmåden, og anvendelse af dette |
| HUE030493T2 (en) | 2003-04-04 | 2017-05-29 | Basf Enzymes Llc | Pectate lyases, nucleic acids and processes encoding them for their production and use |
| US7125842B2 (en) | 2003-04-07 | 2006-10-24 | Canbas Co. Ltd. | Anti-fungal compounds and methods of use |
| CN1798570A (zh) | 2003-04-07 | 2006-07-05 | 坎巴斯有限公司 | 抗真菌肽模拟物 |
| US8158354B2 (en) | 2003-05-13 | 2012-04-17 | Ibis Biosciences, Inc. | Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
| US20140011201A1 (en) | 2003-05-13 | 2014-01-09 | Ibis Biosciences, Inc. | Method for the purification of targeted nucleic acids from background nucleic acids |
| US20050106589A1 (en) | 2003-11-17 | 2005-05-19 | Hashem Akhavan-Tafti | Compositions and methods for releasing nucleic acids from solid phase binding materials |
| CN103333870A (zh) | 2003-12-03 | 2013-10-02 | 丹尼斯科美国公司 | 过水解酶 |
| US20050163825A1 (en) | 2004-01-27 | 2005-07-28 | Naidu A. S. | Lactoferrin-treated filament materials |
| AU2005200670B2 (en) | 2004-02-20 | 2007-05-03 | F. Hoffmann-La Roche Ag | Adsorption of nucleic acids to a solid phase |
| CA2588833A1 (en) | 2004-12-09 | 2007-04-19 | The Dial Corporation | Compositions having a high antiviral and antibacterial efficacy |
| US20080317666A1 (en) * | 2005-02-09 | 2008-12-25 | Elias Fattal | Colonic Delivery of Active Agents |
| US8580192B2 (en) | 2006-10-31 | 2013-11-12 | Ethicon, Inc. | Sterilization of polymeric materials |
| DE102008023297B4 (de) * | 2008-05-08 | 2014-06-26 | Aj Innuscreen Gmbh | Verfahren zur Anreicherung und Isolierung von Nukleinsäuren oder Viren |
| DE102008063003A1 (de) * | 2008-12-23 | 2010-06-24 | Qiagen Gmbh | Nukleinsäureaufreinigungsverfahren |
| AU2015201538A1 (en) | 2009-03-04 | 2015-04-16 | Trustees Of Tufts College | Silk fibroin systems for antibiotic delivery |
| WO2010138074A1 (en) * | 2009-05-29 | 2010-12-02 | Hilborn Joens | Hyaluronic acid based delivery systems |
| US8039613B2 (en) * | 2009-08-28 | 2011-10-18 | Promega Corporation | Methods of purifying a nucleic acid and formulation and kit for use in performing such methods |
| WO2011151428A1 (en) | 2010-06-01 | 2011-12-08 | Qiagen Gmbh | Method for isolating and/or purifying nucleic acid(s) |
| US8716176B2 (en) | 2011-09-19 | 2014-05-06 | Cp Kelco Aps | Low soluble solids acid gels and methods for making same |
| US20140243216A1 (en) | 2011-09-26 | 2014-08-28 | Qiagen Gmbh | Methods for separating nucleic acids by size |
| CN103789297A (zh) | 2012-10-26 | 2014-05-14 | 上海医脉赛科技有限公司 | 快速纯化核酸的方法及试剂盒 |
| WO2015013676A1 (en) | 2013-07-26 | 2015-01-29 | Cepheid | Methods of detecting chlamydia and gonorrhea and of screening for infection/inflammation based on genomic copy number |
| MX2016003963A (es) | 2013-09-30 | 2016-07-06 | Southern Gardens Citrus | Metodo para determinar la calidad y/o seguridad de un jugo/sidra. |
| EP3077072B1 (en) * | 2013-12-02 | 2019-03-06 | Biocartis N.V. | Extraction of circulating nucleic acids |
| US10619152B2 (en) | 2014-02-10 | 2020-04-14 | Nancy Quan | Isolation of cell-free nucleic acids from bodily fluid samples using solid chaotropic agents |
| DK3161136T3 (da) * | 2014-06-24 | 2019-07-01 | Aj Innuscreen Gmbh | Fremgangsmåde til berigelse af mikrovesikler |
| DK3227419T3 (da) | 2014-12-05 | 2019-11-18 | Dalli Werke Gmbh & Co Kg | Brusesammensætninger der omfatter pectin |
| JP6787916B2 (ja) | 2015-06-10 | 2020-11-18 | キアゲン ゲーエムベーハー | アニオン交換粒子を使用する細胞外核酸を単離する方法 |
| CN108219026A (zh) | 2016-12-14 | 2018-06-29 | 杭州垚信生物科技有限公司 | 一种高胶凝甜菜果胶的制备方法 |
| CN107141369B (zh) | 2017-05-25 | 2019-10-18 | 华南理工大学 | 一种改性果胶的制备方法 |
| FR3078977B1 (fr) | 2018-03-16 | 2023-03-17 | Biomerieux Sa | Procede d'extraction d'acides nucleiques |
| US11819584B2 (en) | 2018-08-17 | 2023-11-21 | Cepheid | Nucleic acid decontamination methods |
| WO2020037262A1 (en) | 2018-08-17 | 2020-02-20 | Cepheid | Nucleic acid isolation and related methods |
-
2019
- 2019-08-16 AU AU2019320825A patent/AU2019320825B2/en active Active
- 2019-08-16 CA CA3109805A patent/CA3109805A1/en active Pending
- 2019-08-16 JP JP2021507772A patent/JP7516348B2/ja active Active
- 2019-08-16 CN CN201980065415.8A patent/CN113195714A/zh active Pending
- 2019-08-16 EP EP19762058.6A patent/EP3837363A1/en active Pending
- 2019-08-16 US US17/269,182 patent/US12365891B2/en active Active
- 2019-08-16 KR KR1020217007884A patent/KR102900265B1/ko active Active
- 2019-08-16 WO PCT/US2019/046908 patent/WO2020037260A1/en not_active Ceased
-
2021
- 2021-03-03 ZA ZA2021/01476A patent/ZA202101476B/en unknown
-
2025
- 2025-06-23 US US19/245,885 patent/US20260028613A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US12365891B2 (en) | 2025-07-22 |
| CA3109805A1 (en) | 2020-02-20 |
| JP7516348B2 (ja) | 2024-07-16 |
| AU2019320825B2 (en) | 2025-09-25 |
| US20260028613A1 (en) | 2026-01-29 |
| EP3837363A1 (en) | 2021-06-23 |
| KR102900265B1 (ko) | 2025-12-15 |
| JP2021533780A (ja) | 2021-12-09 |
| AU2019320825A1 (en) | 2021-04-08 |
| CN113195714A (zh) | 2021-07-30 |
| US20210324372A1 (en) | 2021-10-21 |
| WO2020037260A1 (en) | 2020-02-20 |
| KR20210045451A (ko) | 2021-04-26 |
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