JP7516348B2 - 核酸単離のための方法と組成物 - Google Patents
核酸単離のための方法と組成物 Download PDFInfo
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- JP7516348B2 JP7516348B2 JP2021507772A JP2021507772A JP7516348B2 JP 7516348 B2 JP7516348 B2 JP 7516348B2 JP 2021507772 A JP2021507772 A JP 2021507772A JP 2021507772 A JP2021507772 A JP 2021507772A JP 7516348 B2 JP7516348 B2 JP 7516348B2
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- polysaccharide
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- alkyl
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- 108020004707 nucleic acids Proteins 0.000 title claims description 231
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- 238000002955 isolation Methods 0.000 title description 24
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- 239000003795 chemical substances by application Substances 0.000 claims description 118
- 239000001814 pectin Substances 0.000 claims description 111
- 229920001277 pectin Polymers 0.000 claims description 84
- 235000010987 pectin Nutrition 0.000 claims description 82
- 238000003752 polymerase chain reaction Methods 0.000 claims description 51
- 150000003839 salts Chemical class 0.000 claims description 43
- 125000000217 alkyl group Chemical group 0.000 claims description 36
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- PYMYPHUHKUWMLA-UHFFFAOYSA-N 2,3,4,5-tetrahydroxypentanal Chemical group OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 31
- 239000002253 acid Substances 0.000 claims description 29
- 235000019824 amidated pectin Nutrition 0.000 claims description 28
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- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical group N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 12
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical group SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 claims description 12
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- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 claims description 11
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 10
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- 125000000954 2-hydroxyethyl group Chemical group [H]C([*])([H])C([H])([H])O[H] 0.000 claims description 9
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- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical group OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 9
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- 125000006552 (C3-C8) cycloalkyl group Chemical group 0.000 claims description 7
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- 125000000592 heterocycloalkyl group Chemical group 0.000 claims description 7
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- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 claims description 4
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 claims description 4
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- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 claims description 3
- 238000007857 nested PCR Methods 0.000 claims description 3
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 claims description 3
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- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 2
- 210000003722 extracellular fluid Anatomy 0.000 claims description 2
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 claims description 2
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- VLTRZXGMWDSKGL-UHFFFAOYSA-M perchlorate Inorganic materials [O-]Cl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-M 0.000 claims description 2
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- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
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- REFMEZARFCPESH-UHFFFAOYSA-M sodium;heptane-1-sulfonate Chemical compound [Na+].CCCCCCCS([O-])(=O)=O REFMEZARFCPESH-UHFFFAOYSA-M 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0045—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Galacturonans, e.g. methyl ester of (alpha-1,4)-linked D-galacturonic acid units, i.e. pectin, or hydrolysis product of methyl ester of alpha-1,4-linked D-galacturonic acid units, i.e. pectinic acid; Derivatives thereof
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
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| US201862765013P | 2018-08-17 | 2018-08-17 | |
| US62/765,013 | 2018-08-17 | ||
| PCT/US2019/046908 WO2020037260A1 (en) | 2018-08-17 | 2019-08-16 | Methods and compositions for nucleic acid isolation |
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| JP2021533780A5 JP2021533780A5 (https=) | 2022-08-23 |
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| WO (1) | WO2020037260A1 (https=) |
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| CN115197935B (zh) * | 2021-04-08 | 2025-10-21 | 上海细胞治疗集团股份有限公司 | 纤维素色谱纯化rna的方法 |
| CN114807308B (zh) * | 2022-05-07 | 2025-10-10 | 英科新创（苏州）生物科技有限公司 | 核酸释放剂、试剂或试剂盒、其应用及核酸检测方法 |
| CN115873664B (zh) * | 2022-12-06 | 2024-08-13 | 上海领骏生物科技有限公司 | 一种用于核酸提取磁珠的清洗液及其使用方法 |
| WO2025101548A2 (en) | 2023-11-08 | 2025-05-15 | Cepheid | Her2 low quantification method |
| WO2026055639A1 (en) | 2024-09-06 | 2026-03-12 | Cepheid | Sample in sequencing-ready library out automated library preparation solution |
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| JP2012513385A (ja) | 2008-12-23 | 2012-06-14 | キアゲン ゲーエムベーハー | 核酸の精製法 |
| JP2013502934A (ja) | 2009-08-28 | 2013-01-31 | プロメガ コーポレイション | 核酸の精製方法及びこの方法の実施に使用する配合物及びキット |
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| AU682226B2 (en) | 1993-11-23 | 1997-09-25 | Ciba Corning Diagnostics Corp. | Use of antisense oligomers in a process for controlling contamination in nucleic acid amplification reactions |
| GB9927904D0 (en) | 1999-11-25 | 2000-01-26 | Amersham Pharm Biotech Ab | A method fro obtaining a nucleic acid variant |
| US6374684B1 (en) | 2000-08-25 | 2002-04-23 | Cepheid | Fluid control and processing system |
| EP1390514A2 (en) | 2001-05-04 | 2004-02-25 | Ato B.V. | Regulatory protein involved in pectin modification |
| WO2003000776A1 (en) * | 2001-05-14 | 2003-01-03 | Johns Hopkins Singapore Pte Ltd | Biodegradable polyphosphoramidates for controlled release of bioactive substances |
| DK176653B1 (da) | 2002-07-02 | 2009-02-02 | Cp Kelco Aps | Fremgangsmåde til fremstilling af amideret pektin med lav forestringsgrad, amideret pektin, som er opnåeligt ved fremgangsmåden, og anvendelse af dette |
| HUE030493T2 (en) | 2003-04-04 | 2017-05-29 | Basf Enzymes Llc | Pectate lyases, nucleic acids and processes encoding them for their production and use |
| US7125842B2 (en) | 2003-04-07 | 2006-10-24 | Canbas Co. Ltd. | Anti-fungal compounds and methods of use |
| CN1798570A (zh) | 2003-04-07 | 2006-07-05 | 坎巴斯有限公司 | 抗真菌肽模拟物 |
| US8158354B2 (en) | 2003-05-13 | 2012-04-17 | Ibis Biosciences, Inc. | Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
| US20140011201A1 (en) | 2003-05-13 | 2014-01-09 | Ibis Biosciences, Inc. | Method for the purification of targeted nucleic acids from background nucleic acids |
| US20050106589A1 (en) | 2003-11-17 | 2005-05-19 | Hashem Akhavan-Tafti | Compositions and methods for releasing nucleic acids from solid phase binding materials |
| CN103333870A (zh) | 2003-12-03 | 2013-10-02 | 丹尼斯科美国公司 | 过水解酶 |
| US20050163825A1 (en) | 2004-01-27 | 2005-07-28 | Naidu A. S. | Lactoferrin-treated filament materials |
| AU2005200670B2 (en) | 2004-02-20 | 2007-05-03 | F. Hoffmann-La Roche Ag | Adsorption of nucleic acids to a solid phase |
| CA2588833A1 (en) | 2004-12-09 | 2007-04-19 | The Dial Corporation | Compositions having a high antiviral and antibacterial efficacy |
| US20080317666A1 (en) * | 2005-02-09 | 2008-12-25 | Elias Fattal | Colonic Delivery of Active Agents |
| US8580192B2 (en) | 2006-10-31 | 2013-11-12 | Ethicon, Inc. | Sterilization of polymeric materials |
| DE102008023297B4 (de) * | 2008-05-08 | 2014-06-26 | Aj Innuscreen Gmbh | Verfahren zur Anreicherung und Isolierung von Nukleinsäuren oder Viren |
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| JP2012513385A (ja) | 2008-12-23 | 2012-06-14 | キアゲン ゲーエムベーハー | 核酸の精製法 |
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| EP3837363A1 (en) | 2021-06-23 |
| KR102900265B1 (ko) | 2025-12-15 |
| JP2021533780A (ja) | 2021-12-09 |
| ZA202101476B (en) | 2024-08-28 |
| AU2019320825A1 (en) | 2021-04-08 |
| CN113195714A (zh) | 2021-07-30 |
| US20210324372A1 (en) | 2021-10-21 |
| WO2020037260A1 (en) | 2020-02-20 |
| KR20210045451A (ko) | 2021-04-26 |
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