CN113195714A - 核酸分离的方法和组合物 - Google Patents

核酸分离的方法和组合物 Download PDF

Info

Publication number
CN113195714A
CN113195714A CN201980065415.8A CN201980065415A CN113195714A CN 113195714 A CN113195714 A CN 113195714A CN 201980065415 A CN201980065415 A CN 201980065415A CN 113195714 A CN113195714 A CN 113195714A
Authority
CN
China
Prior art keywords
nucleic acid
polysaccharide
pectin
sample
kda
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201980065415.8A
Other languages
English (en)
Chinese (zh)
Inventor
V·巴拉兹尼诺克
A·I·库蒂亚文
O·Z·纳纳西
D·塞尔盖耶夫
A·A·高尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cepheid
Original Assignee
Cepheid
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cepheid filed Critical Cepheid
Publication of CN113195714A publication Critical patent/CN113195714A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0045—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Galacturonans, e.g. methyl ester of (alpha-1,4)-linked D-galacturonic acid units, i.e. pectin, or hydrolysis product of methyl ester of alpha-1,4-linked D-galacturonic acid units, i.e. pectinic acid; Derivatives thereof

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201980065415.8A 2018-08-17 2019-08-16 核酸分离的方法和组合物 Pending CN113195714A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862765013P 2018-08-17 2018-08-17
US62/765013 2018-08-17
PCT/US2019/046908 WO2020037260A1 (en) 2018-08-17 2019-08-16 Methods and compositions for nucleic acid isolation

Publications (1)

Publication Number Publication Date
CN113195714A true CN113195714A (zh) 2021-07-30

Family

ID=67809720

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201980065415.8A Pending CN113195714A (zh) 2018-08-17 2019-08-16 核酸分离的方法和组合物

Country Status (9)

Country Link
US (2) US12365891B2 (https=)
EP (1) EP3837363A1 (https=)
JP (1) JP7516348B2 (https=)
KR (1) KR102900265B1 (https=)
CN (1) CN113195714A (https=)
AU (1) AU2019320825B2 (https=)
CA (1) CA3109805A1 (https=)
WO (1) WO2020037260A1 (https=)
ZA (1) ZA202101476B (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115873664A (zh) * 2022-12-06 2023-03-31 上海领骏生物科技有限公司 一种用于核酸提取磁珠的清洗液及其使用方法

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113281503B (zh) * 2020-10-29 2022-01-04 杭州微策生物技术股份有限公司 一种用于裂解病毒样本的试剂
CN115197935B (zh) * 2021-04-08 2025-10-21 上海细胞治疗集团股份有限公司 纤维素色谱纯化rna的方法
CN114807308B (zh) * 2022-05-07 2025-10-10 英科新创（苏州）生物科技有限公司 核酸释放剂、试剂或试剂盒、其应用及核酸检测方法
WO2025101548A2 (en) 2023-11-08 2025-05-15 Cepheid Her2 low quantification method
WO2026055639A1 (en) 2024-09-06 2026-03-12 Cepheid Sample in sequencing-ready library out automated library preparation solution

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050119450A1 (en) * 2001-05-14 2005-06-02 Jun Wang Biodegradable polyphosphoramidates for controlled release of bioactive substances
US20080317666A1 (en) * 2005-02-09 2008-12-25 Elias Fattal Colonic Delivery of Active Agents
WO2010138074A1 (en) * 2009-05-29 2010-12-02 Hilborn Joens Hyaluronic acid based delivery systems
US20110054161A1 (en) * 2009-08-28 2011-03-03 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
US20110117628A1 (en) * 2008-05-08 2011-05-19 Aj Innuscreen Gmbh Method for concentrating and isolating biomolecules or viruses
US20120245337A1 (en) * 2008-12-23 2012-09-27 Qiagen Gmbh Nucleic acid purification method
US20170335312A1 (en) * 2014-06-24 2017-11-23 Aj Innuscreen Gmbh Method for enriching microvesicles
CN113015800A (zh) * 2018-08-17 2021-06-22 塞弗德公司 核酸分离及相关方法
CN113056291A (zh) * 2018-08-17 2021-06-29 塞弗德公司 核酸去污方法

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1264321B (it) 1992-01-13 1996-09-23 Mini Ricerca Scient Tecnolog Esteri di acidi pectici e pectinici, procedimento di preparazione e loro applicazioni farmaceutiche e biosanitarie
AU682226B2 (en) 1993-11-23 1997-09-25 Ciba Corning Diagnostics Corp. Use of antisense oligomers in a process for controlling contamination in nucleic acid amplification reactions
GB9927904D0 (en) 1999-11-25 2000-01-26 Amersham Pharm Biotech Ab A method fro obtaining a nucleic acid variant
US6374684B1 (en) 2000-08-25 2002-04-23 Cepheid Fluid control and processing system
EP1390514A2 (en) 2001-05-04 2004-02-25 Ato B.V. Regulatory protein involved in pectin modification
DK176653B1 (da) 2002-07-02 2009-02-02 Cp Kelco Aps Fremgangsmåde til fremstilling af amideret pektin med lav forestringsgrad, amideret pektin, som er opnåeligt ved fremgangsmåden, og anvendelse af dette
HUE030493T2 (en) 2003-04-04 2017-05-29 Basf Enzymes Llc Pectate lyases, nucleic acids and processes encoding them for their production and use
US7125842B2 (en) 2003-04-07 2006-10-24 Canbas Co. Ltd. Anti-fungal compounds and methods of use
CN1798570A (zh) 2003-04-07 2006-07-05 坎巴斯有限公司 抗真菌肽模拟物
US8158354B2 (en) 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US20140011201A1 (en) 2003-05-13 2014-01-09 Ibis Biosciences, Inc. Method for the purification of targeted nucleic acids from background nucleic acids
US20050106589A1 (en) 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Compositions and methods for releasing nucleic acids from solid phase binding materials
CN103333870A (zh) 2003-12-03 2013-10-02 丹尼斯科美国公司 过水解酶
US20050163825A1 (en) 2004-01-27 2005-07-28 Naidu A. S. Lactoferrin-treated filament materials
AU2005200670B2 (en) 2004-02-20 2007-05-03 F. Hoffmann-La Roche Ag Adsorption of nucleic acids to a solid phase
CA2588833A1 (en) 2004-12-09 2007-04-19 The Dial Corporation Compositions having a high antiviral and antibacterial efficacy
US8580192B2 (en) 2006-10-31 2013-11-12 Ethicon, Inc. Sterilization of polymeric materials
AU2015201538A1 (en) 2009-03-04 2015-04-16 Trustees Of Tufts College Silk fibroin systems for antibiotic delivery
WO2011151428A1 (en) 2010-06-01 2011-12-08 Qiagen Gmbh Method for isolating and/or purifying nucleic acid(s)
US8716176B2 (en) 2011-09-19 2014-05-06 Cp Kelco Aps Low soluble solids acid gels and methods for making same
US20140243216A1 (en) 2011-09-26 2014-08-28 Qiagen Gmbh Methods for separating nucleic acids by size
CN103789297A (zh) 2012-10-26 2014-05-14 上海医脉赛科技有限公司 快速纯化核酸的方法及试剂盒
WO2015013676A1 (en) 2013-07-26 2015-01-29 Cepheid Methods of detecting chlamydia and gonorrhea and of screening for infection/inflammation based on genomic copy number
MX2016003963A (es) 2013-09-30 2016-07-06 Southern Gardens Citrus Metodo para determinar la calidad y/o seguridad de un jugo/sidra.
EP3077072B1 (en) * 2013-12-02 2019-03-06 Biocartis N.V. Extraction of circulating nucleic acids
US10619152B2 (en) 2014-02-10 2020-04-14 Nancy Quan Isolation of cell-free nucleic acids from bodily fluid samples using solid chaotropic agents
DK3227419T3 (da) 2014-12-05 2019-11-18 Dalli Werke Gmbh & Co Kg Brusesammensætninger der omfatter pectin
JP6787916B2 (ja) 2015-06-10 2020-11-18 キアゲン ゲーエムベーハー アニオン交換粒子を使用する細胞外核酸を単離する方法
CN108219026A (zh) 2016-12-14 2018-06-29 杭州垚信生物科技有限公司 一种高胶凝甜菜果胶的制备方法
CN107141369B (zh) 2017-05-25 2019-10-18 华南理工大学 一种改性果胶的制备方法
FR3078977B1 (fr) 2018-03-16 2023-03-17 Biomerieux Sa Procede d'extraction d'acides nucleiques

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050119450A1 (en) * 2001-05-14 2005-06-02 Jun Wang Biodegradable polyphosphoramidates for controlled release of bioactive substances
US20080317666A1 (en) * 2005-02-09 2008-12-25 Elias Fattal Colonic Delivery of Active Agents
US20110117628A1 (en) * 2008-05-08 2011-05-19 Aj Innuscreen Gmbh Method for concentrating and isolating biomolecules or viruses
US20120245337A1 (en) * 2008-12-23 2012-09-27 Qiagen Gmbh Nucleic acid purification method
WO2010138074A1 (en) * 2009-05-29 2010-12-02 Hilborn Joens Hyaluronic acid based delivery systems
US20110054161A1 (en) * 2009-08-28 2011-03-03 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
US20170335312A1 (en) * 2014-06-24 2017-11-23 Aj Innuscreen Gmbh Method for enriching microvesicles
CN113015800A (zh) * 2018-08-17 2021-06-22 塞弗德公司 核酸分离及相关方法
CN113056291A (zh) * 2018-08-17 2021-06-29 塞弗德公司 核酸去污方法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
KHALED，等: "One-pot synthesis of pH-responsive hybrid nanogel particles for the intracellular delivery of small interfering RNA", BIOMATERIALS, vol. 87, 31 January 2016 (2016-01-31), pages 57 - 68, XP029453174, DOI: 10.1016/j.biomaterials.2016.01.052 *
MARCELLO LENUCCI，等: "Do polyamines contribute to plant cell wall assembly by forming amide bonds with pectins?", PHYTOCHEMISTRY, vol. 66, 18 October 2005 (2005-10-18), pages 2583 *
PRESTWICH: "Hyaluronic acid-based clinical biomaterials derived for cell and molecule delivery in regenerative medicine", JOURNAL OFCONTROLLED RELEASE, vol. 155, 30 April 2011 (2011-04-30), pages 193 - 199, XP028315147, DOI: 10.1016/j.jconrel.2011.04.007 *
李巧巧，等: "酰胺化低酯果胶的研究进展及产业现状", 安徽农业科学, vol. 37, no. 21, 31 December 2009 (2009-12-31), pages 10148 - 101150 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115873664A (zh) * 2022-12-06 2023-03-31 上海领骏生物科技有限公司 一种用于核酸提取磁珠的清洗液及其使用方法

Also Published As

Publication number Publication date
US12365891B2 (en) 2025-07-22
CA3109805A1 (en) 2020-02-20
JP7516348B2 (ja) 2024-07-16
AU2019320825B2 (en) 2025-09-25
US20260028613A1 (en) 2026-01-29
EP3837363A1 (en) 2021-06-23
KR102900265B1 (ko) 2025-12-15
JP2021533780A (ja) 2021-12-09
ZA202101476B (en) 2024-08-28
AU2019320825A1 (en) 2021-04-08
US20210324372A1 (en) 2021-10-21
WO2020037260A1 (en) 2020-02-20
KR20210045451A (ko) 2021-04-26

Similar Documents

Publication Publication Date Title
KR102900265B1 (ko) 핵산 분리를 위한 방법 및 조성물
US12312582B2 (en) Method for isolation of nucleic acid containing particles and extraction of nucleic acids therefrom
US20060234251A1 (en) Methods of enhancing isolation of RNA from biological samples
US20070185322A1 (en) Methods of extracting RNA
WO2007100934A2 (en) Methods and compositions for the rapid isolation of small rna molecules
US20120142001A1 (en) Method for isolation of nucleic acid containing particles and extraction of nucleic acids therefrom
WO2012023569A1 (ja) Ｒｎａ抽出用溶液
US20070190526A1 (en) Methods of extracting nucleic acids
JP2021533780A5 (https=)
EP3837364A1 (en) Nucleic acid isolation and related methods
JP6121780B2 (ja) 生物学的試料の選択的調製のためのアミン化合物
JP2021528089A (ja) 試料調製法およびシステム
CN101400689A (zh) 提取rna的方法
Zhou et al. Detection of dsRNA with fluorescence in situ hybridization (FISH)
EP4299765A1 (en) Method for detecting and quantifying nucleic acid from environmental samples
JPWO2006088030A1 (ja) 核酸抽出方法、核酸抽出剤、および核酸抽出用キット

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination