AU2019320825B2 - Methods and compositions for nucleic acid isolation - Google Patents

Methods and compositions for nucleic acid isolation

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Publication number
AU2019320825B2
AU2019320825B2 AU2019320825A AU2019320825A AU2019320825B2 AU 2019320825 B2 AU2019320825 B2 AU 2019320825B2 AU 2019320825 A AU2019320825 A AU 2019320825A AU 2019320825 A AU2019320825 A AU 2019320825A AU 2019320825 B2 AU2019320825 B2 AU 2019320825B2
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AU
Australia
Prior art keywords
nucleic acid
polysaccharide
kda
pectin
sample
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AU2019320825A
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English (en)
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AU2019320825A1 (en
Inventor
Vera BARAZNENOK
Alexander A. Gall
Alex I. KUTYAVIN
Oliver Z. Nanassy
Dmitri Sergueev
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Cepheid
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Cepheid
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Publication of AU2019320825A1 publication Critical patent/AU2019320825A1/en
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Publication of AU2019320825B2 publication Critical patent/AU2019320825B2/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08BPOLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0045Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Galacturonans, e.g. methyl ester of (alpha-1,4)-linked D-galacturonic acid units, i.e. pectin, or hydrolysis product of methyl ester of alpha-1,4-linked D-galacturonic acid units, i.e. pectinic acid; Derivatives thereof

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2019320825A 2018-08-17 2019-08-16 Methods and compositions for nucleic acid isolation Active AU2019320825B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862765013P 2018-08-17 2018-08-17
US62/765,013 2018-08-17
PCT/US2019/046908 WO2020037260A1 (en) 2018-08-17 2019-08-16 Methods and compositions for nucleic acid isolation

Publications (2)

Publication Number Publication Date
AU2019320825A1 AU2019320825A1 (en) 2021-04-08
AU2019320825B2 true AU2019320825B2 (en) 2025-09-25

Family

ID=67809720

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2019320825A Active AU2019320825B2 (en) 2018-08-17 2019-08-16 Methods and compositions for nucleic acid isolation

Country Status (9)

Country Link
US (2) US12365891B2 (https=)
EP (1) EP3837363A1 (https=)
JP (1) JP7516348B2 (https=)
KR (1) KR102900265B1 (https=)
CN (1) CN113195714A (https=)
AU (1) AU2019320825B2 (https=)
CA (1) CA3109805A1 (https=)
WO (1) WO2020037260A1 (https=)
ZA (1) ZA202101476B (https=)

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CN113281503B (zh) * 2020-10-29 2022-01-04 杭州微策生物技术股份有限公司 一种用于裂解病毒样本的试剂
CN115197935B (zh) * 2021-04-08 2025-10-21 上海细胞治疗集团股份有限公司 纤维素色谱纯化rna的方法
CN114807308B (zh) * 2022-05-07 2025-10-10 英科新创(苏州)生物科技有限公司 核酸释放剂、试剂或试剂盒、其应用及核酸检测方法
CN115873664B (zh) * 2022-12-06 2024-08-13 上海领骏生物科技有限公司 一种用于核酸提取磁珠的清洗液及其使用方法
WO2025101548A2 (en) 2023-11-08 2025-05-15 Cepheid Her2 low quantification method
WO2026055639A1 (en) 2024-09-06 2026-03-12 Cepheid Sample in sequencing-ready library out automated library preparation solution

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US20120245337A1 (en) * 2008-12-23 2012-09-27 Qiagen Gmbh Nucleic acid purification method
US20170335312A1 (en) * 2014-06-24 2017-11-23 Aj Innuscreen Gmbh Method for enriching microvesicles

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AU682226B2 (en) 1993-11-23 1997-09-25 Ciba Corning Diagnostics Corp. Use of antisense oligomers in a process for controlling contamination in nucleic acid amplification reactions
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WO2003000776A1 (en) * 2001-05-14 2003-01-03 Johns Hopkins Singapore Pte Ltd Biodegradable polyphosphoramidates for controlled release of bioactive substances
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HUE030493T2 (en) 2003-04-04 2017-05-29 Basf Enzymes Llc Pectate lyases, nucleic acids and processes encoding them for their production and use
US7125842B2 (en) 2003-04-07 2006-10-24 Canbas Co. Ltd. Anti-fungal compounds and methods of use
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US20170335312A1 (en) * 2014-06-24 2017-11-23 Aj Innuscreen Gmbh Method for enriching microvesicles

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Also Published As

Publication number Publication date
US12365891B2 (en) 2025-07-22
CA3109805A1 (en) 2020-02-20
JP7516348B2 (ja) 2024-07-16
US20260028613A1 (en) 2026-01-29
EP3837363A1 (en) 2021-06-23
KR102900265B1 (ko) 2025-12-15
JP2021533780A (ja) 2021-12-09
ZA202101476B (en) 2024-08-28
AU2019320825A1 (en) 2021-04-08
CN113195714A (zh) 2021-07-30
US20210324372A1 (en) 2021-10-21
WO2020037260A1 (en) 2020-02-20
KR20210045451A (ko) 2021-04-26

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