ZA200602938B - Cloning of cytochrome p450 genes from Nicotiana - Google Patents

Cloning of cytochrome p450 genes from Nicotiana Download PDF

Info

Publication number
ZA200602938B
ZA200602938B ZA200602938A ZA200602938A ZA200602938B ZA 200602938 B ZA200602938 B ZA 200602938B ZA 200602938 A ZA200602938 A ZA 200602938A ZA 200602938 A ZA200602938 A ZA 200602938A ZA 200602938 B ZA200602938 B ZA 200602938B
Authority
ZA
South Africa
Prior art keywords
seq
nucleic acid
plant
amino acid
acid seq
Prior art date
Application number
ZA200602938A
Other languages
English (en)
Inventor
Dongmei Xu
Original Assignee
Us Smokeless Tobacco Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/686,947 external-priority patent/US20040162420A1/en
Application filed by Us Smokeless Tobacco Company filed Critical Us Smokeless Tobacco Company
Publication of ZA200602938B publication Critical patent/ZA200602938B/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0012Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042NADPH-cytochrome P450 reductase (1.6.2.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ZA200602938A 2003-10-16 2004-10-15 Cloning of cytochrome p450 genes from Nicotiana ZA200602938B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US10/686,947 US20040162420A1 (en) 2002-10-16 2003-10-16 Cloning of cytochrome p450 genes from nicotiana

Publications (1)

Publication Number Publication Date
ZA200602938B true ZA200602938B (en) 2008-06-25

Family

ID=37426131

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA200602938A ZA200602938B (en) 2003-10-16 2004-10-15 Cloning of cytochrome p450 genes from Nicotiana

Country Status (4)

Country Link
US (3) US8058504B2 (zh)
EP (1) EP2330191A1 (zh)
CN (2) CN1926235B (zh)
ZA (1) ZA200602938B (zh)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7812227B2 (en) * 2001-11-13 2010-10-12 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
US8592663B2 (en) 2001-11-13 2013-11-26 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US10266836B2 (en) 2001-11-13 2019-04-23 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US8637731B2 (en) * 2003-10-16 2014-01-28 U.S. Smokeless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
CN1926235B (zh) 2003-10-16 2012-07-18 美国无烟烟草有限责任公司 从烟草克隆细胞色素p450基因
US8586837B2 (en) * 2004-04-29 2013-11-19 U.S. Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
US20070199097A1 (en) * 2004-09-03 2007-08-23 U.S. Smokeless Tobacco Company Tobacco plants having a mutation in a nicotine demethylase gene
ATE530656T1 (de) 2005-02-23 2011-11-15 Univ North Carolina State Veränderung des alkaloidgehaltes in tabak durch modifikation spezifischer cytochrome p450 gene.
US8319011B2 (en) 2006-12-15 2012-11-27 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
US11332753B2 (en) 2006-12-15 2022-05-17 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
KR100794395B1 (ko) * 2007-01-19 2008-01-15 명지대학교 산학협력단 식물의 종자 크기를 증가시키는 시토크롬 p450 유전자
BRPI0820042B1 (pt) 2007-11-12 2020-05-19 North Carolina State University método de obtenção de uma planta de tabaco, ou célula ou parte desta, tendo níveis reduzidos de nornicotina, produto de tabaco, método para fabricar um produto de tabaco, polinucleotídeo isolado, cassete de expressão, polipetpídeo isolado e método de obtenção de uma planta, ou parte de planta desta, do gênero nicotiana
EA033565B1 (ru) 2010-01-15 2019-11-05 Univ North Carolina State Композиции и способы, предназначенные для минимизации синтеза норникотина в растениях табака
CN103687480A (zh) 2011-02-28 2014-03-26 北卡罗莱纳州立大学 烟草近交植物ncbex1f、ncbex1ms和nc ex90
JP2014511184A (ja) 2011-02-28 2014-05-15 アルトリア クライアント サービシーズ インコーポレイテッド タバコ近交系植物albex1f及びalbex1ms
DK2806754T3 (en) * 2012-01-23 2019-02-18 Dsm Ip Assets Bv Diterpene PREPARATION
US9603335B2 (en) 2013-01-11 2017-03-28 North Carolina State University Tobacco inbred plants K326 SRC, CMS K326 SRC, K346 SRC, CMS K346 SRC, NC1562-1 SRC, NCTG-61 SRC, CMS NCTG-61 SRC and hybrid NC196 SRC
WO2014137802A1 (en) 2013-03-05 2014-09-12 North Carolina State University Tobacco inbred and hybrid plants and uses thereof
US9596822B2 (en) 2014-03-03 2017-03-21 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
JP2017508462A (ja) 2014-03-03 2017-03-30 ノース カロライナ ステイト ユニヴァーシティ タバコ近交系植物およびハイブリッド植物ならびにそれらから作られたタバコ製品
EP3113602A1 (en) 2014-03-03 2017-01-11 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
WO2016046288A1 (en) * 2014-09-26 2016-03-31 Philip Morris Products S.A Reducing tobacco specific nitrosamines through alteration of the nitrate assimilation pathway
CN113774035A (zh) * 2021-09-01 2021-12-10 广东省农业科学院果树研究所 一种细胞色素P450酶及其编码基因CsCYP2与应用

Family Cites Families (79)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4801541A (en) * 1981-09-11 1989-01-31 The University Of Rochester Method of increasing the yield of a product by altering a microorganism
US4762785A (en) 1982-08-12 1988-08-09 Calgene, Inc. Novel method and compositions for introducting alien DNA in vivo
EP0320500B1 (en) 1983-01-13 2004-11-17 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Non-oncogenic ti plasmid vector system and recombinant DNA molecules for the introduction of expressible genes into plant cell genomes
WO1984002919A1 (en) 1983-01-17 1984-08-02 Monsanto Co Plasmids for transforming plant cells
US5034322A (en) * 1983-01-17 1991-07-23 Monsanto Company Chimeric genes suitable for expression in plant cells
US5352605A (en) * 1983-01-17 1994-10-04 Monsanto Company Chimeric genes for transforming plant cells using viral promoters
NL8300699A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; werkwijze voor het produceren van agrobacterium tumefaciens bacterien; stabiele cointegraat plasmiden; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
NL8300698A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
US4732856A (en) * 1984-04-03 1988-03-22 Carnegie Institution Of Washington Transposable elements and process for using same
NL8401048A (nl) 1984-04-03 1985-11-01 Rijksuniversiteit Leiden En Pr Werkwijze voor het inbouwen van vreemd dna in het genoom van eenzaadlobbige planten.
US5231019A (en) 1984-05-11 1993-07-27 Ciba-Geigy Corporation Transformation of hereditary material of plants
US5149645A (en) 1984-06-04 1992-09-22 Rijksuniversiteit Leiden Process for introducing foreign DNA into the genome of plants
NL8401780A (nl) 1984-06-04 1986-01-02 Rijksuniversiteit Leiden En Pr Werkwijze voor het inbouwen van vreemd dna in het genoom van planten.
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5107065A (en) * 1986-03-28 1992-04-21 Calgene, Inc. Anti-sense regulation of gene expression in plant cells
EP0265502A1 (en) 1986-04-30 1988-05-04 Boyce Thompson Institute For Plant Research, Inc. Electric field mediated dna transformation of plant cells and organelles
US5188958A (en) 1986-05-29 1993-02-23 Calgene, Inc. Transformation and foreign gene expression in brassica species
US5177010A (en) 1986-06-30 1993-01-05 University Of Toledo Process for transforming corn and the products thereof
US4801540A (en) * 1986-10-17 1989-01-31 Calgene, Inc. PG gene and its use in plants
EP0267159A3 (de) 1986-11-07 1990-05-02 Ciba-Geigy Ag Verfahren zur genetischen Modifikation monokotyler Pflanzen
SE455438B (sv) 1986-11-24 1988-07-11 Aga Ab Sett att senka en brennares flamtemperatur samt brennare med munstycken for oxygen resp brensle
US5004863B2 (en) 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
US5350689A (en) * 1987-05-20 1994-09-27 Ciba-Geigy Corporation Zea mays plants and transgenic Zea mays plants regenerated from protoplasts or protoplast-derived cells
EP0513849B1 (en) 1987-05-20 1998-08-19 Novartis AG Method of preparing transgenic Zea mays plants regenerated from protoplasts or protoplast-derived cells
US5013658A (en) * 1988-05-13 1991-05-07 Dna Plant Technology Corporation Transposon tagging of genes in transformed plants
ES2060765T3 (es) * 1988-05-17 1994-12-01 Lubrizol Genetics Inc Sistema promotor de ubiquitina en plantas.
US5034323A (en) * 1989-03-30 1991-07-23 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US5141131A (en) 1989-06-30 1992-08-25 Dowelanco Method and apparatus for the acceleration of a propellable matter
DK0426641T3 (da) * 1989-10-31 2000-10-23 Monsanto Co Promotor til transgene planter
WO1992009696A1 (en) * 1990-11-23 1992-06-11 Plant Genetic Systems, N.V. Process for transforming monocotyledonous plants
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
US5378142A (en) 1991-04-12 1995-01-03 Engelhard Corporation Combustion process using catalysts containing binary oxides
PT626998E (pt) 1992-02-19 2002-04-29 State Of Oregon By S B Hi Educ "producao de plantas resistentes aos virus, mediante introducao de uma molecula de arn viral nao traduzivel e de sentido codificador"
WO1993021335A2 (en) 1992-04-15 1993-10-28 Plant Genetic Systems, N.V. Transformation of monocot cells
ATE398679T1 (de) 1992-07-07 2008-07-15 Japan Tobacco Inc Verfahren zur transformation einer monokotyledon pflanze
US5469976A (en) 1993-04-30 1995-11-28 Burchell; James R. Shelf allocation and management system
EP0627752B1 (en) 1993-06-04 1997-07-23 CAVIS S.r.l. An inertia device for interrupting the electrical circuit of a vehicle with an internal combustion engine
ES2326705T3 (es) * 1995-09-14 2009-10-16 Virginia Tech Intellectual Properties, Inc. Produccion de enzimas lisosomicas en sistemas de expresion basados en plantas.
CN1249779A (zh) * 1997-03-07 2000-04-05 诺瓦提斯公司 细胞色素p450单加氧酶
WO1998040470A2 (en) * 1997-03-07 1998-09-17 Novartis Ag Cytochrome p450 monooxygenases
US7238860B2 (en) * 2001-04-18 2007-07-03 Mendel Biotechnology, Inc. Yield-related polynucleotides and polypeptides in plants
EP1173547A4 (en) * 1999-02-11 2004-09-15 Univ Arizona DWF4 POLYNUCLEOTIDES, POLYPEPTIDES AND ITS USES
EP1033405A3 (en) 1999-02-25 2001-08-01 Ceres Incorporated Sequence-determined DNA fragments and corresponding polypeptides encoded thereby
US6781033B2 (en) * 2000-04-26 2004-08-24 Monsanto Technology Llc Method for the transformation of plant cell plastids
CA2954889A1 (en) 2001-03-09 2002-09-19 University Of Kentucky Research Foundation Cytochrome p450s and uses thereof
NZ530238A (en) * 2001-06-08 2004-08-27 Vector Tobacco Ltd Modifying nicotine and nitrosamine levels in tobacco
US6730832B1 (en) 2001-09-10 2004-05-04 Luis Mayan Dominguez High threonine producing lines of Nicotiana tobacum and methods for producing
US7032601B2 (en) * 2001-09-28 2006-04-25 U.S. Smokeless Tobacco Company Encapsulated materials
US6953040B2 (en) * 2001-09-28 2005-10-11 U.S. Smokeless Tobacco Company Tobacco mint plant material product
US7184992B1 (en) 2001-11-01 2007-02-27 George Mason Intellectual Properties, Inc. Constrained optimization tool
US20040117869A1 (en) 2002-01-11 2004-06-17 U.S. Smokeless Tobacco Company Cloning of cytochrome P450 genes from Nicotiana
US7700851B2 (en) * 2001-11-13 2010-04-20 U.S. Smokeless Tobacco Company Tobacco nicotine demethylase genomic clone and uses thereof
WO2005038018A2 (en) 2003-10-16 2005-04-28 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
US20040111759A1 (en) * 2001-11-13 2004-06-10 U.S. Smokeless Tobacco Company Identification and use of cytochrome P450 nucleic acid sequences from tobacco
US8592663B2 (en) 2001-11-13 2013-11-26 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US20040162420A1 (en) * 2002-10-16 2004-08-19 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
US7700834B2 (en) * 2001-11-13 2010-04-20 U.S. Smokless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
US20040103449A1 (en) * 2001-11-13 2004-05-27 U.S. Smokeless Tobacco Company Identification and use of cytochrome P450 nucleic acid sequences from tobacco
US7812227B2 (en) * 2001-11-13 2010-10-12 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
US7855318B2 (en) * 2001-11-13 2010-12-21 U.S. Smokeless Tobacco Company Cloning of cytochrome P450 genes from Nicotiana
WO2005038033A2 (en) 2003-10-16 2005-04-28 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
CA2477452A1 (en) * 2002-03-12 2003-09-25 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
WO2004035745A2 (en) * 2002-10-16 2004-04-29 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
US8637731B2 (en) 2003-10-16 2014-01-28 U.S. Smokeless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
CN1926235B (zh) 2003-10-16 2012-07-18 美国无烟烟草有限责任公司 从烟草克隆细胞色素p450基因
US8627828B2 (en) * 2003-11-07 2014-01-14 U.S. Smokeless Tobacco Company Llc Tobacco compositions
CN102669810B (zh) 2003-11-07 2014-11-05 美国无烟烟草有限责任公司 烟草组合物
TW200531647A (en) * 2003-12-22 2005-10-01 Us Smokeless Tobacco Co Conditioning process for tobacco and/or snuff compositions
WO2005111217A2 (en) 2004-04-29 2005-11-24 U.S. Smokeless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
US8586837B2 (en) 2004-04-29 2013-11-19 U.S. Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
US20060185686A1 (en) * 2004-08-23 2006-08-24 Lawrence Robert H Jr Nicotiana diversity
US7798153B2 (en) * 2004-08-23 2010-09-21 Us Smokeless Tobacco Co. Nicotiana Kawakamii smokeless tobacco
US20070199097A1 (en) 2004-09-03 2007-08-23 U.S. Smokeless Tobacco Company Tobacco plants having a mutation in a nicotine demethylase gene
ATE530656T1 (de) 2005-02-23 2011-11-15 Univ North Carolina State Veränderung des alkaloidgehaltes in tabak durch modifikation spezifischer cytochrome p450 gene.
EP1909603B1 (en) 2005-04-29 2009-10-21 Philip Morris Products S.A. Tobacco pouch product
US7757697B2 (en) * 2005-12-22 2010-07-20 Swisher International, Inc. Method for reducing nitrosamines in tobacco
US8319011B2 (en) 2006-12-15 2012-11-27 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
BRPI0820042B1 (pt) 2007-11-12 2020-05-19 North Carolina State University método de obtenção de uma planta de tabaco, ou célula ou parte desta, tendo níveis reduzidos de nornicotina, produto de tabaco, método para fabricar um produto de tabaco, polinucleotídeo isolado, cassete de expressão, polipetpídeo isolado e método de obtenção de uma planta, ou parte de planta desta, do gênero nicotiana

Also Published As

Publication number Publication date
US8058504B2 (en) 2011-11-15
CN1867674A (zh) 2006-11-22
CN1926235B (zh) 2012-07-18
CN1867674B (zh) 2011-11-09
EP2330191A1 (en) 2011-06-08
US8658856B2 (en) 2014-02-25
US20120011622A1 (en) 2012-01-12
US8188337B2 (en) 2012-05-29
CN1926235A (zh) 2007-03-07
US20100261192A1 (en) 2010-10-14
US20120233727A1 (en) 2012-09-13

Similar Documents

Publication Publication Date Title
AU2010201696B2 (en) Cloning of cytochrome P450 genes from nicotiana
US8188337B2 (en) Cloning of cytochrome p450 genes from Nicotiana
US9464297B2 (en) Cloning of cytochrome P450 genes from nicotiana
US7855318B2 (en) Cloning of cytochrome P450 genes from Nicotiana
US20070292871A1 (en) Method of screening for cytochrome p450s
EP2204444A1 (en) Cloning of cytochrome P450 genes from Nicotiana
US20080076126A1 (en) Cloning of cytochrome p450 genes from nicotiana
EP1694832B1 (en) Use of a cytochrome p450 gene from nicotiana
EP1572911A2 (en) Cloning of cytochrome p450 genes from nicotiana
JP5558797B2 (ja) タバコ属(Nicotiana)からのチトクロムp450遺伝子のクローニング