ZA200602938B - Cloning of cytochrome p450 genes from Nicotiana - Google Patents

Cloning of cytochrome p450 genes from Nicotiana Download PDF

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Publication number
ZA200602938B
ZA200602938B ZA200602938A ZA200602938A ZA200602938B ZA 200602938 B ZA200602938 B ZA 200602938B ZA 200602938 A ZA200602938 A ZA 200602938A ZA 200602938 A ZA200602938 A ZA 200602938A ZA 200602938 B ZA200602938 B ZA 200602938B
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South Africa
Prior art keywords
seq
nucleic acid
plant
amino acid
acid seq
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ZA200602938A
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English (en)
Inventor
Dongmei Xu
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Us Smokeless Tobacco Company
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Publication date
Priority claimed from US10/686,947 external-priority patent/US20040162420A1/en
Application filed by Us Smokeless Tobacco Company filed Critical Us Smokeless Tobacco Company
Publication of ZA200602938B publication Critical patent/ZA200602938B/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0012Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042NADPH-cytochrome P450 reductase (1.6.2.4)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ZA200602938A 2003-10-16 2004-10-15 Cloning of cytochrome p450 genes from Nicotiana ZA200602938B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US10/686,947 US20040162420A1 (en) 2002-10-16 2003-10-16 Cloning of cytochrome p450 genes from nicotiana

Publications (1)

Publication Number Publication Date
ZA200602938B true ZA200602938B (en) 2008-06-25

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ID=37426131

Family Applications (1)

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ZA200602938A ZA200602938B (en) 2003-10-16 2004-10-15 Cloning of cytochrome p450 genes from Nicotiana

Country Status (4)

Country Link
US (3) US8058504B2 (zh)
EP (1) EP2330191A1 (zh)
CN (2) CN1867674B (zh)
ZA (1) ZA200602938B (zh)

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CN1867674B (zh) 2003-10-16 2011-11-09 美国无烟烟草有限责任公司 从烟草克隆细胞色素p450基因
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US20070199097A1 (en) * 2004-09-03 2007-08-23 U.S. Smokeless Tobacco Company Tobacco plants having a mutation in a nicotine demethylase gene
BRPI0519985B1 (pt) 2005-02-23 2018-01-30 University Of Kentucky Research Foundation Método para redução do nível de nornicotina ou n’-nitrosonornicotina em uma planta do gênero nicotiana ou parte desta ou em um produto de tabaco, cassete de expressão e método para a produção de uma planta do gênero nicotiana
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US11332753B2 (en) 2006-12-15 2022-05-17 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
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EP2220231B1 (en) 2007-11-12 2017-10-11 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
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CA2828488A1 (en) 2011-02-28 2012-09-07 North Carolina State University Tobacco inbred plants ncbex1f, ncbex1ms, and nc ex90
CN104203005A (zh) * 2012-01-23 2014-12-10 帝斯曼知识产权资产管理有限公司 二萜的生产
US9603335B2 (en) 2013-01-11 2017-03-28 North Carolina State University Tobacco inbred plants K326 SRC, CMS K326 SRC, K346 SRC, CMS K346 SRC, NC1562-1 SRC, NCTG-61 SRC, CMS NCTG-61 SRC and hybrid NC196 SRC
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CN106998787A (zh) * 2014-09-26 2017-08-01 菲利普莫里斯生产公司 通过改变硝酸同化路径减少烟草特异性亚硝胺
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Also Published As

Publication number Publication date
CN1867674B (zh) 2011-11-09
US20120011622A1 (en) 2012-01-12
CN1926235A (zh) 2007-03-07
US20100261192A1 (en) 2010-10-14
US8188337B2 (en) 2012-05-29
US8658856B2 (en) 2014-02-25
US8058504B2 (en) 2011-11-15
CN1867674A (zh) 2006-11-22
US20120233727A1 (en) 2012-09-13
EP2330191A1 (en) 2011-06-08
CN1926235B (zh) 2012-07-18

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