ZA200602938B - Cloning of cytochrome p450 genes from Nicotiana - Google Patents
Cloning of cytochrome p450 genes from Nicotiana Download PDFInfo
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- ZA200602938B ZA200602938B ZA200602938A ZA200602938A ZA200602938B ZA 200602938 B ZA200602938 B ZA 200602938B ZA 200602938 A ZA200602938 A ZA 200602938A ZA 200602938 A ZA200602938 A ZA 200602938A ZA 200602938 B ZA200602938 B ZA 200602938B
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
- C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
- C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
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- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
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- General Health & Medical Sciences (AREA)
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- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
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US10/686,947 US20040162420A1 (en) | 2002-10-16 | 2003-10-16 | Cloning of cytochrome p450 genes from nicotiana |
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ZA200602938B true ZA200602938B (en) | 2008-06-25 |
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ZA200602938A ZA200602938B (en) | 2003-10-16 | 2004-10-15 | Cloning of cytochrome p450 genes from Nicotiana |
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EP (1) | EP2330191A1 (zh) |
CN (2) | CN1926235B (zh) |
ZA (1) | ZA200602938B (zh) |
Families Citing this family (23)
Publication number | Priority date | Publication date | Assignee | Title |
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US7812227B2 (en) * | 2001-11-13 | 2010-10-12 | U.S. Smokeless Tobacco Company | Cloning of cytochrome p450 genes from nicotiana |
US8592663B2 (en) | 2001-11-13 | 2013-11-26 | U.S. Smokeless Tobacco Company Llc | Tobacco nicotine demethylase genomic clone and uses thereof |
US10266836B2 (en) | 2001-11-13 | 2019-04-23 | U.S. Smokeless Tobacco Company Llc | Tobacco nicotine demethylase genomic clone and uses thereof |
US8637731B2 (en) * | 2003-10-16 | 2014-01-28 | U.S. Smokeless Tobacco Company | Nicotiana nucleic acid molecules and uses thereof |
CN1926235B (zh) | 2003-10-16 | 2012-07-18 | 美国无烟烟草有限责任公司 | 从烟草克隆细胞色素p450基因 |
US8586837B2 (en) * | 2004-04-29 | 2013-11-19 | U.S. Smokeless Tobacco Company Llc | Nicotiana nucleic acid molecules and uses thereof |
US20070199097A1 (en) * | 2004-09-03 | 2007-08-23 | U.S. Smokeless Tobacco Company | Tobacco plants having a mutation in a nicotine demethylase gene |
ATE530656T1 (de) | 2005-02-23 | 2011-11-15 | Univ North Carolina State | Veränderung des alkaloidgehaltes in tabak durch modifikation spezifischer cytochrome p450 gene. |
US8319011B2 (en) | 2006-12-15 | 2012-11-27 | U.S. Smokeless Tobacco Company Llc | Tobacco plants having reduced nicotine demethylase activity |
US11332753B2 (en) | 2006-12-15 | 2022-05-17 | U.S. Smokeless Tobacco Company Llc | Tobacco plants having reduced nicotine demethylase activity |
KR100794395B1 (ko) * | 2007-01-19 | 2008-01-15 | 명지대학교 산학협력단 | 식물의 종자 크기를 증가시키는 시토크롬 p450 유전자 |
BRPI0820042B1 (pt) | 2007-11-12 | 2020-05-19 | North Carolina State University | método de obtenção de uma planta de tabaco, ou célula ou parte desta, tendo níveis reduzidos de nornicotina, produto de tabaco, método para fabricar um produto de tabaco, polinucleotídeo isolado, cassete de expressão, polipetpídeo isolado e método de obtenção de uma planta, ou parte de planta desta, do gênero nicotiana |
EA033565B1 (ru) | 2010-01-15 | 2019-11-05 | Univ North Carolina State | Композиции и способы, предназначенные для минимизации синтеза норникотина в растениях табака |
CN103687480A (zh) | 2011-02-28 | 2014-03-26 | 北卡罗莱纳州立大学 | 烟草近交植物ncbex1f、ncbex1ms和nc ex90 |
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2004
- 2004-10-15 CN CN200480030387XA patent/CN1926235B/zh active Active
- 2004-10-15 EP EP10012983A patent/EP2330191A1/en not_active Withdrawn
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US8058504B2 (en) | 2011-11-15 |
CN1867674A (zh) | 2006-11-22 |
CN1926235B (zh) | 2012-07-18 |
CN1867674B (zh) | 2011-11-09 |
EP2330191A1 (en) | 2011-06-08 |
US8658856B2 (en) | 2014-02-25 |
US20120011622A1 (en) | 2012-01-12 |
US8188337B2 (en) | 2012-05-29 |
CN1926235A (zh) | 2007-03-07 |
US20100261192A1 (en) | 2010-10-14 |
US20120233727A1 (en) | 2012-09-13 |
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