ZA200602938B - Cloning of cytochrome p450 genes from Nicotiana - Google Patents
Cloning of cytochrome p450 genes from Nicotiana Download PDFInfo
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- ZA200602938B ZA200602938B ZA200602938A ZA200602938A ZA200602938B ZA 200602938 B ZA200602938 B ZA 200602938B ZA 200602938 A ZA200602938 A ZA 200602938A ZA 200602938 A ZA200602938 A ZA 200602938A ZA 200602938 B ZA200602938 B ZA 200602938B
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
- C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
- C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
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- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
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- General Health & Medical Sciences (AREA)
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- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
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| US10/686,947 US20040162420A1 (en) | 2002-10-16 | 2003-10-16 | Cloning of cytochrome p450 genes from nicotiana |
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| ZA200602938B true ZA200602938B (en) | 2008-06-25 |
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| ZA200602938A ZA200602938B (en) | 2003-10-16 | 2004-10-15 | Cloning of cytochrome p450 genes from Nicotiana |
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| EP (1) | EP2330191A1 (zh) |
| CN (2) | CN1867674B (zh) |
| ZA (1) | ZA200602938B (zh) |
Families Citing this family (23)
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| US10266836B2 (en) | 2001-11-13 | 2019-04-23 | U.S. Smokeless Tobacco Company Llc | Tobacco nicotine demethylase genomic clone and uses thereof |
| US8592663B2 (en) | 2001-11-13 | 2013-11-26 | U.S. Smokeless Tobacco Company Llc | Tobacco nicotine demethylase genomic clone and uses thereof |
| US7812227B2 (en) | 2001-11-13 | 2010-10-12 | U.S. Smokeless Tobacco Company | Cloning of cytochrome p450 genes from nicotiana |
| US8637731B2 (en) * | 2003-10-16 | 2014-01-28 | U.S. Smokeless Tobacco Company | Nicotiana nucleic acid molecules and uses thereof |
| CN1867674B (zh) | 2003-10-16 | 2011-11-09 | 美国无烟烟草有限责任公司 | 从烟草克隆细胞色素p450基因 |
| US8586837B2 (en) | 2004-04-29 | 2013-11-19 | U.S. Smokeless Tobacco Company Llc | Nicotiana nucleic acid molecules and uses thereof |
| US20070199097A1 (en) * | 2004-09-03 | 2007-08-23 | U.S. Smokeless Tobacco Company | Tobacco plants having a mutation in a nicotine demethylase gene |
| BRPI0519985B1 (pt) | 2005-02-23 | 2018-01-30 | University Of Kentucky Research Foundation | Método para redução do nível de nornicotina ou n-nitrosonornicotina em uma planta do gênero nicotiana ou parte desta ou em um produto de tabaco, cassete de expressão e método para a produção de uma planta do gênero nicotiana |
| US8319011B2 (en) | 2006-12-15 | 2012-11-27 | U.S. Smokeless Tobacco Company Llc | Tobacco plants having reduced nicotine demethylase activity |
| US11332753B2 (en) | 2006-12-15 | 2022-05-17 | U.S. Smokeless Tobacco Company Llc | Tobacco plants having reduced nicotine demethylase activity |
| KR100794395B1 (ko) * | 2007-01-19 | 2008-01-15 | 명지대학교 산학협력단 | 식물의 종자 크기를 증가시키는 시토크롬 ｐ４５０ 유전자 |
| EP2220231B1 (en) | 2007-11-12 | 2017-10-11 | North Carolina State University | Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes |
| CN102858983B (zh) | 2010-01-15 | 2015-08-12 | 北卡罗来纳州立大学 | 最小化烟草中去甲烟碱合成的组合物和方法 |
| CA2828484A1 (en) | 2011-02-28 | 2012-09-07 | Altria Client Services Inc. | Tobacco inbred plants albex1f and albex1ms |
| CA2828488A1 (en) | 2011-02-28 | 2012-09-07 | North Carolina State University | Tobacco inbred plants ncbex1f, ncbex1ms, and nc ex90 |
| CN104203005A (zh) * | 2012-01-23 | 2014-12-10 | 帝斯曼知识产权资产管理有限公司 | 二萜的生产 |
| US9603335B2 (en) | 2013-01-11 | 2017-03-28 | North Carolina State University | Tobacco inbred plants K326 SRC, CMS K326 SRC, K346 SRC, CMS K346 SRC, NC1562-1 SRC, NCTG-61 SRC, CMS NCTG-61 SRC and hybrid NC196 SRC |
| US9560830B2 (en) | 2013-03-05 | 2017-02-07 | North Carolina State University | Tobacco inbred and hybrid plants and uses thereof |
| US9596823B2 (en) | 2014-03-03 | 2017-03-21 | North Carolina State University | Tobacco inbred and hybrid plants and tobacco products made thereof |
| WO2015134427A1 (en) | 2014-03-03 | 2015-09-11 | North Carolina State University | Tobacco inbred and hybrid plants and tobacco products made thereof |
| EP3113603A1 (en) | 2014-03-03 | 2017-01-11 | North Carolina State University | Tobacco inbred and hybrid plants and tobacco products made thereof |
| CN106998787A (zh) * | 2014-09-26 | 2017-08-01 | 菲利普莫里斯生产公司 | 通过改变硝酸同化路径减少烟草特异性亚硝胺 |
| CN113774035A (zh) * | 2021-09-01 | 2021-12-10 | 广东省农业科学院果树研究所 | 一种细胞色素P450酶及其编码基因CsCYP2与应用 |
Family Cites Families (79)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4801541A (en) * | 1981-09-11 | 1989-01-31 | The University Of Rochester | Method of increasing the yield of a product by altering a microorganism |
| US4762785A (en) | 1982-08-12 | 1988-08-09 | Calgene, Inc. | Novel method and compositions for introducting alien DNA in vivo |
| ATE52800T1 (de) | 1983-01-13 | 1990-06-15 | Max Planck Gesellschaft | Verfahren zum einbringen von expressionsfaehigen genen in pflanzenzellgenome und hybride ti plasmidvektoren enthaltende agrobacterium-staemme verwendbar in diesem verfahren. |
| US5352605A (en) * | 1983-01-17 | 1994-10-04 | Monsanto Company | Chimeric genes for transforming plant cells using viral promoters |
| EP0131624B1 (en) | 1983-01-17 | 1992-09-16 | Monsanto Company | Plasmids for transforming plant cells |
| US5034322A (en) * | 1983-01-17 | 1991-07-23 | Monsanto Company | Chimeric genes suitable for expression in plant cells |
| NL8300699A (nl) | 1983-02-24 | 1984-09-17 | Univ Leiden | Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; werkwijze voor het produceren van agrobacterium tumefaciens bacterien; stabiele cointegraat plasmiden; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten. |
| NL8300698A (nl) | 1983-02-24 | 1984-09-17 | Univ Leiden | Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten. |
| NL8401048A (nl) | 1984-04-03 | 1985-11-01 | Rijksuniversiteit Leiden En Pr | Werkwijze voor het inbouwen van vreemd dna in het genoom van eenzaadlobbige planten. |
| US4732856A (en) * | 1984-04-03 | 1988-03-22 | Carnegie Institution Of Washington | Transposable elements and process for using same |
| US5231019A (en) | 1984-05-11 | 1993-07-27 | Ciba-Geigy Corporation | Transformation of hereditary material of plants |
| US5149645A (en) | 1984-06-04 | 1992-09-22 | Rijksuniversiteit Leiden | Process for introducing foreign DNA into the genome of plants |
| NL8401780A (nl) | 1984-06-04 | 1986-01-02 | Rijksuniversiteit Leiden En Pr | Werkwijze voor het inbouwen van vreemd dna in het genoom van planten. |
| US4945050A (en) | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
| US5107065A (en) * | 1986-03-28 | 1992-04-21 | Calgene, Inc. | Anti-sense regulation of gene expression in plant cells |
| EP0265502A1 (en) | 1986-04-30 | 1988-05-04 | Boyce Thompson Institute For Plant Research, Inc. | Electric field mediated dna transformation of plant cells and organelles |
| US5188958A (en) | 1986-05-29 | 1993-02-23 | Calgene, Inc. | Transformation and foreign gene expression in brassica species |
| US5177010A (en) | 1986-06-30 | 1993-01-05 | University Of Toledo | Process for transforming corn and the products thereof |
| US4801540A (en) * | 1986-10-17 | 1989-01-31 | Calgene, Inc. | PG gene and its use in plants |
| EP0267159A3 (de) | 1986-11-07 | 1990-05-02 | Ciba-Geigy Ag | Verfahren zur genetischen Modifikation monokotyler Pflanzen |
| SE455438B (sv) | 1986-11-24 | 1988-07-11 | Aga Ab | Sett att senka en brennares flamtemperatur samt brennare med munstycken for oxygen resp brensle |
| US5004863B2 (en) | 1986-12-03 | 2000-10-17 | Agracetus | Genetic engineering of cotton plants and lines |
| EP0846771A1 (en) | 1987-05-20 | 1998-06-10 | Novartis AG | Zea mays plants and transgenic zea mays plants regenerated from protoplasts or protoplast-derived cells |
| US5350689A (en) * | 1987-05-20 | 1994-09-27 | Ciba-Geigy Corporation | Zea mays plants and transgenic Zea mays plants regenerated from protoplasts or protoplast-derived cells |
| US5013658A (en) * | 1988-05-13 | 1991-05-07 | Dna Plant Technology Corporation | Transposon tagging of genes in transformed plants |
| ES2060765T3 (es) * | 1988-05-17 | 1994-12-01 | Lubrizol Genetics Inc | Sistema promotor de ubiquitina en plantas. |
| US5034323A (en) * | 1989-03-30 | 1991-07-23 | Dna Plant Technology Corporation | Genetic engineering of novel plant phenotypes |
| US5302523A (en) | 1989-06-21 | 1994-04-12 | Zeneca Limited | Transformation of plant cells |
| US5141131A (en) | 1989-06-30 | 1992-08-25 | Dowelanco | Method and apparatus for the acceleration of a propellable matter |
| ES2150900T3 (es) * | 1989-10-31 | 2000-12-16 | Monsanto Co | Promotor para plantas transgenicas. |
| JP3234598B2 (ja) * | 1990-11-23 | 2001-12-04 | プラント・ジエネテイツク・システムズ・エヌ・ベー | 単子葉植物の形質転換方法 |
| US5384253A (en) | 1990-12-28 | 1995-01-24 | Dekalb Genetics Corporation | Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes |
| US5378142A (en) | 1991-04-12 | 1995-01-03 | Engelhard Corporation | Combustion process using catalysts containing binary oxides |
| ES2166361T3 (es) | 1992-02-19 | 2002-04-16 | State Of Oregon Acting By & Th | Produccion de plantas resistentes a los virus a traves de la introduccion de rna viral intraducible de sentido positivo. |
| JPH07505531A (ja) * | 1992-04-15 | 1995-06-22 | プラント・ジェネティック・システムズ・エヌ・ブイ | 単子葉植物細胞の形質転換法 |
| EP0604662B1 (en) | 1992-07-07 | 2008-06-18 | Japan Tobacco Inc. | Method of transforming monocotyledon |
| US5469976A (en) | 1993-04-30 | 1995-11-28 | Burchell; James R. | Shelf allocation and management system |
| EP0627752B1 (en) | 1993-06-04 | 1997-07-23 | CAVIS S.r.l. | An inertia device for interrupting the electrical circuit of a vehicle with an internal combustion engine |
| ES2326705T3 (es) * | 1995-09-14 | 2009-10-16 | Virginia Tech Intellectual Properties, Inc. | Produccion de enzimas lisosomicas en sistemas de expresion basados en plantas. |
| HUP0002926A3 (en) * | 1997-03-07 | 2005-11-28 | Syngenta Participations Ag | Cytochrome p450 monooxygenases |
| WO1998040470A2 (en) * | 1997-03-07 | 1998-09-17 | Novartis Ag | Cytochrome p450 monooxygenases |
| US7238860B2 (en) * | 2001-04-18 | 2007-07-03 | Mendel Biotechnology, Inc. | Yield-related polynucleotides and polypeptides in plants |
| AU4001000A (en) * | 1999-02-11 | 2000-08-29 | Arizona Board Of Regents On Behalf Of The University Of Arizona, The | Dwf4 polynucleotides, polypeptides and uses thereof |
| EP1033405A3 (en) | 1999-02-25 | 2001-08-01 | Ceres Incorporated | Sequence-determined DNA fragments and corresponding polypeptides encoded thereby |
| WO2001081605A2 (en) * | 2000-04-26 | 2001-11-01 | Monsanto Technology Llc | Method for the transformation of plant cell plastids |
| US7405057B2 (en) | 2001-03-09 | 2008-07-29 | Board Of Trustees Of The University Of Kentucky | Cytochrome P450s and uses thereof |
| CA2449920A1 (en) * | 2001-06-08 | 2002-12-19 | Vector Tobacco Ltd. | Modifying nicotine and nitrosamine levels in tobacco |
| US6730832B1 (en) | 2001-09-10 | 2004-05-04 | Luis Mayan Dominguez | High threonine producing lines of Nicotiana tobacum and methods for producing |
| US6953040B2 (en) * | 2001-09-28 | 2005-10-11 | U.S. Smokeless Tobacco Company | Tobacco mint plant material product |
| US7032601B2 (en) * | 2001-09-28 | 2006-04-25 | U.S. Smokeless Tobacco Company | Encapsulated materials |
| US7184992B1 (en) | 2001-11-01 | 2007-02-27 | George Mason Intellectual Properties, Inc. | Constrained optimization tool |
| US20040162420A1 (en) * | 2002-10-16 | 2004-08-19 | U.S. Smokeless Tobacco Company | Cloning of cytochrome p450 genes from nicotiana |
| US8592663B2 (en) | 2001-11-13 | 2013-11-26 | U.S. Smokeless Tobacco Company Llc | Tobacco nicotine demethylase genomic clone and uses thereof |
| US20040111759A1 (en) | 2001-11-13 | 2004-06-10 | U.S. Smokeless Tobacco Company | Identification and use of cytochrome P450 nucleic acid sequences from tobacco |
| US20040103449A1 (en) * | 2001-11-13 | 2004-05-27 | U.S. Smokeless Tobacco Company | Identification and use of cytochrome P450 nucleic acid sequences from tobacco |
| WO2005038033A2 (en) | 2003-10-16 | 2005-04-28 | U.S. Smokeless Tobacco Company | Cloning of cytochrome p450 genes from nicotiana |
| US20040117869A1 (en) * | 2002-01-11 | 2004-06-17 | U.S. Smokeless Tobacco Company | Cloning of cytochrome P450 genes from Nicotiana |
| US7700834B2 (en) * | 2001-11-13 | 2010-04-20 | U.S. Smokless Tobacco Company | Nicotiana nucleic acid molecules and uses thereof |
| US7700851B2 (en) * | 2001-11-13 | 2010-04-20 | U.S. Smokeless Tobacco Company | Tobacco nicotine demethylase genomic clone and uses thereof |
| US7812227B2 (en) * | 2001-11-13 | 2010-10-12 | U.S. Smokeless Tobacco Company | Cloning of cytochrome p450 genes from nicotiana |
| US7855318B2 (en) * | 2001-11-13 | 2010-12-21 | U.S. Smokeless Tobacco Company | Cloning of cytochrome P450 genes from Nicotiana |
| AP2004003122A0 (en) * | 2002-03-12 | 2004-09-30 | Us Smokeless Tobacco Co | Cloning of cytochrome P450 genes from nicotiana |
| EP1581633A4 (en) * | 2002-10-16 | 2008-10-15 | Us Smokeless Tobacco Co | CLONING GENES OF CYTOCHROME P450 DERIVATIVES OF PLANTS NICOTIANA |
| EP1694832B1 (en) | 2003-10-16 | 2012-04-18 | U.S. Smokeless Tobacco Company LLC | Use of a cytochrome p450 gene from nicotiana |
| US8637731B2 (en) | 2003-10-16 | 2014-01-28 | U.S. Smokeless Tobacco Company | Nicotiana nucleic acid molecules and uses thereof |
| CN1867674B (zh) | 2003-10-16 | 2011-11-09 | 美国无烟烟草有限责任公司 | 从烟草克隆细胞色素p450基因 |
| JP4931596B2 (ja) | 2003-11-07 | 2012-05-16 | ユーエス スモークレス タバコ カンパニー リミテッド ライアビリティ カンパニー | タバコ組成物 |
| US8627828B2 (en) * | 2003-11-07 | 2014-01-14 | U.S. Smokeless Tobacco Company Llc | Tobacco compositions |
| EP1729602A1 (en) * | 2003-12-22 | 2006-12-13 | U.S. Smokeless Tobacco Company | Conditioning process for tobacco and/or snuff compositions |
| PL1751278T3 (pl) | 2004-04-29 | 2015-08-31 | U S Smokeless Tobacco Company Llc | Cząsteczki kwasu nukleinowego Nicotiana i ich zastosowanie |
| US8586837B2 (en) | 2004-04-29 | 2013-11-19 | U.S. Smokeless Tobacco Company Llc | Nicotiana nucleic acid molecules and uses thereof |
| US20060185686A1 (en) * | 2004-08-23 | 2006-08-24 | Lawrence Robert H Jr | Nicotiana diversity |
| WO2006022784A1 (en) | 2004-08-23 | 2006-03-02 | U.S. Smokeless Tobacco Company | Nicotiana compositions |
| US20070199097A1 (en) * | 2004-09-03 | 2007-08-23 | U.S. Smokeless Tobacco Company | Tobacco plants having a mutation in a nicotine demethylase gene |
| BRPI0519985B1 (pt) | 2005-02-23 | 2018-01-30 | University Of Kentucky Research Foundation | Método para redução do nível de nornicotina ou n-nitrosonornicotina em uma planta do gênero nicotiana ou parte desta ou em um produto de tabaco, cassete de expressão e método para a produção de uma planta do gênero nicotiana |
| DE602006009944D1 (de) | 2005-04-29 | 2009-12-03 | Philip Morris Prod | Tabakbeutelprodukt |
| US7757697B2 (en) * | 2005-12-22 | 2010-07-20 | Swisher International, Inc. | Method for reducing nitrosamines in tobacco |
| US8319011B2 (en) | 2006-12-15 | 2012-11-27 | U.S. Smokeless Tobacco Company Llc | Tobacco plants having reduced nicotine demethylase activity |
| EP2220231B1 (en) | 2007-11-12 | 2017-10-11 | North Carolina State University | Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes |
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2004
- 2004-10-15 CN CN2004800303850A patent/CN1867674B/zh active Active
- 2004-10-15 CN CN200480030387XA patent/CN1926235B/zh active Active
- 2004-10-15 EP EP10012983A patent/EP2330191A1/en not_active Withdrawn
- 2004-10-15 ZA ZA200602938A patent/ZA200602938B/en unknown
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- 2010-06-23 US US12/821,273 patent/US8058504B2/en not_active Expired - Fee Related
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2011
- 2011-09-23 US US13/243,270 patent/US8188337B2/en not_active Expired - Lifetime
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2012
- 2012-05-25 US US13/481,297 patent/US8658856B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CN1867674B (zh) | 2011-11-09 |
| US20120011622A1 (en) | 2012-01-12 |
| CN1926235A (zh) | 2007-03-07 |
| US20100261192A1 (en) | 2010-10-14 |
| US8188337B2 (en) | 2012-05-29 |
| US8658856B2 (en) | 2014-02-25 |
| US8058504B2 (en) | 2011-11-15 |
| CN1867674A (zh) | 2006-11-22 |
| US20120233727A1 (en) | 2012-09-13 |
| EP2330191A1 (en) | 2011-06-08 |
| CN1926235B (zh) | 2012-07-18 |
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