ZA200600017B - Insecticidal proteins secreted from Bacillus thuringiensis and uses therefor - Google Patents
Insecticidal proteins secreted from Bacillus thuringiensis and uses therefor Download PDFInfo
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- ZA200600017B ZA200600017B ZA200600017A ZA200600017A ZA200600017B ZA 200600017 B ZA200600017 B ZA 200600017B ZA 200600017 A ZA200600017 A ZA 200600017A ZA 200600017 A ZA200600017 A ZA 200600017A ZA 200600017 B ZA200600017 B ZA 200600017B
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US48548303P | 2003-07-07 | 2003-07-07 |
Publications (1)
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|---|---|
| ZA200600017B true ZA200600017B (en) | 2007-01-31 |
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|---|---|---|---|
| ZA200600017A ZA200600017B (en) | 2003-07-07 | 2006-01-03 | Insecticidal proteins secreted from Bacillus thuringiensis and uses therefor |
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|---|---|
| US (1) | US8796026B2 (ru) |
| EP (1) | EP1644480B1 (ru) |
| CN (1) | CN1849397B (ru) |
| AR (1) | AR048471A1 (ru) |
| AU (1) | AU2004267355B2 (ru) |
| BR (1) | BRPI0412390A (ru) |
| CA (1) | CA2531400A1 (ru) |
| EC (1) | ECSP066270A (ru) |
| MX (1) | MXPA06000293A (ru) |
| RU (1) | RU2382822C2 (ru) |
| WO (1) | WO2005019414A2 (ru) |
| ZA (1) | ZA200600017B (ru) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE602004030044D1 (de) | 2003-12-16 | 2010-12-23 | Monsanto Technology Llc | Bacillus thuringiensis und deren verwendungen |
| PL1818405T3 (pl) | 2004-04-09 | 2015-11-30 | Monsanto Technology Llc | Kompozycje i sposoby do zwalczania inwazji szkodników u roślin |
| US8609936B2 (en) | 2007-04-27 | 2013-12-17 | Monsanto Technology Llc | Hemipteran-and coleopteran active toxin proteins from Bacillus thuringiensis |
| CN104263740A (zh) * | 2008-07-02 | 2015-01-07 | 阿森尼克斯公司 | Axmi-il5、axmi-113、axmi-005、axmi-163 和axmi-184∶vip3a 杀虫蛋白及其使用方法 |
| US8513493B2 (en) * | 2008-08-29 | 2013-08-20 | Monsanto Technology Llc | Hemipteran and coleopteran active toxin proteins from Bacillus thuringiensis |
| US20100199383A1 (en) * | 2008-09-18 | 2010-08-05 | Pioneer Hi-Bred International, Inc. | Novel Bacillus Thuringiensis Gene with Coleopteran Activity |
| CN102421905B (zh) * | 2009-02-27 | 2018-01-23 | 阿森尼克斯公司 | 杀虫蛋白和使用它们的方法 |
| UA111592C2 (uk) * | 2010-07-07 | 2016-05-25 | Сінгента Партісіпейшнс Аг | Спосіб контролю над твердокрилими комахами-шкідниками |
| UY33854A (es) * | 2010-12-30 | 2012-07-31 | Dow Agrosciences Llc | Moléculas de ácido nucleico que se dirigen a la subunidad c de la atpasa vacuolar y confieren resistencia a las plagas de coleópteros |
| NZ612323A (en) * | 2010-12-30 | 2015-12-24 | Dow Agrosciences Llc | Nucleic acid molecules that confer resistance to coleopteran pests |
| US9328356B2 (en) | 2011-02-11 | 2016-05-03 | Monsanto Technology Llc | Pesticidal nucleic acids and proteins and uses thereof |
| CN102399731A (zh) * | 2011-12-12 | 2012-04-04 | 西南大学 | 一株用于防治马铃薯甲虫的苏云金芽孢杆菌 |
| CN102533841B (zh) * | 2012-02-13 | 2013-06-05 | 中国农业科学院生物技术研究所 | 一种提高Bt杀虫蛋白在汉逊酵母中表达量的方法 |
| MD4196C1 (ru) * | 2012-04-27 | 2013-09-30 | Институт Зоологии Академии Наук Молдовы | Штамм бактерий Bacillus thuringiensis subsp. kurstaki, - биоинсектицид для борьбы с жуками-долгоносиками |
| CN102786584B (zh) * | 2012-08-02 | 2013-12-18 | 北京大北农科技集团股份有限公司 | 杀虫蛋白质、其编码基因及用途 |
| CN102796182B (zh) * | 2012-08-02 | 2013-12-18 | 北京大北农科技集团股份有限公司 | 杀虫蛋白质、其编码基因及用途 |
| CN102796183B (zh) * | 2012-08-02 | 2013-12-18 | 北京大北农科技集团股份有限公司 | 杀虫蛋白质、其编码基因及用途 |
| BR112015008504A2 (pt) | 2012-10-15 | 2017-08-22 | Pioneer Hi Bred Int | Método para aumentar a atividade pesticida, polipeptídeo, molécula de ácido nucleico, cassete de expressão, vetor, método de obtenção de uma planta, método de obtenção de uma célula de planta, composição pesticida, método para proteção de uma planta, método para proteger uma planta, método para aumentar a resistência de uma planta |
| US10968446B2 (en) | 2012-11-01 | 2021-04-06 | Massachusetts Institute Of Technology | Directed evolution of synthetic gene cluster |
| CN108998471B (zh) | 2013-03-14 | 2022-11-04 | 先锋国际良种公司 | 用以防治昆虫害虫的组合物和方法 |
| CA2901316A1 (en) | 2013-03-15 | 2014-09-25 | Pioneer Hi-Bred International, Inc. | Phi-4 polypeptides and methods for their use |
| US9974302B2 (en) * | 2013-08-09 | 2018-05-22 | Monsanto Technology Llc | Pesticidal toxin active against coleopteran and/or hemipteran insects |
| EP3032942B1 (en) | 2013-08-16 | 2020-03-11 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| EA031651B1 (ru) | 2013-09-13 | 2019-02-28 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидные белки и способы их применения |
| US10480007B2 (en) | 2014-02-07 | 2019-11-19 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants |
| RU2747978C2 (ru) | 2014-02-07 | 2021-05-18 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидные белки и способы их применения |
| CN103814956B (zh) * | 2014-03-04 | 2015-12-09 | 中南林业科技大学 | 油茶饼粕提取物与苏云金杆菌复配杀虫剂及其制备方法 |
| EP3122882A4 (en) * | 2014-03-28 | 2018-02-28 | Monsanto Technology LLC | Pesticidal toxin proteins active against coleopteran insects |
| WO2016000237A1 (en) | 2014-07-03 | 2016-01-07 | Pioneer Overseas Corporation | Plants having enhanced tolerance to insect pests and related constructs and methods involving insect tolerance genes |
| WO2016044092A1 (en) | 2014-09-17 | 2016-03-24 | Pioneer Hi Bred International Inc | Compositions and methods to control insect pests |
| AR102272A1 (es) * | 2014-10-15 | 2017-02-15 | Pioneer Hi Bred Int | Polipéptidos insecticidas que tienen actividad de amplio espectro y usos de estos |
| CN114736275A (zh) * | 2014-10-16 | 2022-07-12 | 先锋国际良种公司 | 具有改进的活性谱的杀昆虫多肽及其用途 |
| RU2733898C2 (ru) | 2014-10-16 | 2020-10-09 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидные белки и способы их применения |
| EP3267796B1 (en) | 2015-03-11 | 2023-08-09 | Pioneer Hi-Bred International, Inc. | Insecticidal combinations of pip-72 and methods of use |
| WO2016186986A1 (en) | 2015-05-19 | 2016-11-24 | Pioneer Hi Bred International Inc | Insecticidal proteins and methods for their use |
| CN107771181A (zh) | 2015-06-16 | 2018-03-06 | 先锋国际良种公司 | 用以防治昆虫有害生物的组合物和方法 |
| MA49662A (fr) * | 2015-06-22 | 2020-06-03 | Agbiome Inc | Gènes pesticides et leurs procédés d'utilisation |
| EP3322679A4 (en) | 2015-07-13 | 2019-07-10 | Pivot Bio, Inc. | METHODS AND COMPOSITIONS FOR IMPROVING THE CHARACTERISTICS OF A PLANT |
| MX2018001258A (es) * | 2015-07-30 | 2018-04-20 | Monsanto Technology Llc | Proteinas inhibidoras de insectos novedosas. |
| EP3943602A1 (en) | 2015-08-06 | 2022-01-26 | Pioneer Hi-Bred International, Inc. | Plant derived insecticidal proteins and methods for their use |
| CA2992488A1 (en) | 2015-08-28 | 2017-03-09 | Pioneer Hi-Bred International, Inc. | Ochrobactrum-mediated transformation of plants |
| CN108291219B (zh) | 2015-10-05 | 2023-02-17 | 麻省理工学院 | 使用重构nif簇的氮固定 |
| WO2017105987A1 (en) | 2015-12-18 | 2017-06-22 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| UA126966C2 (uk) | 2016-04-14 | 2023-03-01 | Піонір Хай-Бред Інтернешнл, Інк. | Інсектицидний поліпептид, що характеризується поліпшеним спектром активності, та його застосування |
| CA3021391A1 (en) | 2016-04-19 | 2017-10-26 | Pioneer Hi-Bred International, Inc. | Insecticidal combinations of polypeptides having improved activity spectrum and uses thereof |
| MX2018013249A (es) | 2016-05-04 | 2019-02-13 | Pioneer Hi Bred Int | Proteinas insecticidas y metodos para sus usos. |
| US20190185867A1 (en) | 2016-06-16 | 2019-06-20 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
| CN109642237B (zh) | 2016-06-24 | 2022-09-30 | 先锋国际良种公司 | 植物调节元件及其使用方法 |
| MX2018015906A (es) | 2016-07-01 | 2019-04-04 | Pioneer Hi Bred Int | Proteinas insecticidas de plantas y metodos para sus usos. |
| US20210292778A1 (en) | 2016-07-12 | 2021-09-23 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
| AR109205A1 (es) | 2016-08-05 | 2018-11-07 | Syngenta Participations Ag | Control de plagas de coleópteros utilizando moléculas de arn |
| CN111196844B (zh) * | 2016-09-21 | 2021-11-30 | 中国农业科学院植物保护研究所 | 一种杀虫蛋白及其核酸、制备方法和应用 |
| BR112019008800A2 (pt) | 2016-11-01 | 2019-07-16 | Pioneer Hi Bred Int | polipeptídeo inseticida, composição inseticida, polinucleotídeo recombinante, construto de dna, célula de planta ou planta transgênica, método para inibir o crescimento ou exterminar uma população de praga de inseto agrícola, método para inibir o crescimento ou exterminar uma praga de inseto, método para controlar infestação de inseto lepidoptera e/ou coleoptera em uma planta transgênica e fornecer gerenciamento de resistência de inseto e uso de pelo menos um polipeptídeo inseticida |
| CN110088123B (zh) | 2016-12-14 | 2023-10-20 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
| CA3046226A1 (en) | 2016-12-22 | 2018-06-28 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| BR112019014378A2 (pt) | 2017-01-12 | 2020-02-11 | Pivot Bio, Inc. | Métodos e composições para o aprimoramento de traços de plantas |
| WO2018140214A1 (en) | 2017-01-24 | 2018-08-02 | Pioneer Hi-Bred International, Inc. | Nematicidal protein from pseudomonas |
| BR112019016394A2 (pt) | 2017-02-08 | 2020-04-07 | Pioneer Hi Bred Int | construto de dna, pilha molecular, pilha de melhoramento, planta transgênica ou progênie da mesma, composição e método para controlar uma população de praga de inseto |
| CN110914438A (zh) | 2017-05-26 | 2020-03-24 | 先锋国际良种公司 | 具有改善的活性谱的杀昆虫多肽及其用途 |
| US20200165626A1 (en) | 2017-10-13 | 2020-05-28 | Pioneer Hi-Bred International, Inc. | Virus-induced gene silencing technology for insect control in maize |
| WO2019165245A1 (en) | 2018-02-22 | 2019-08-29 | Zymergen Inc. | Method for creating a genomic library enriched for bacillus and identification of novel cry toxins |
| BR112020017975A2 (pt) | 2018-03-02 | 2020-12-29 | Zymergen Inc. | Plataforma de descoberta de proteína inseticida e proteínas inseticidas descobertas a partir da mesma |
| CA3087861A1 (en) | 2018-03-02 | 2019-09-06 | Pioneer Hi-Bred International, Inc. | Plant health assay |
| US11702668B2 (en) | 2018-05-22 | 2023-07-18 | Pioneer Hi-Bred International, Inc. | Plant regulatory elements and methods of use thereof |
| MX2020013875A (es) | 2018-06-27 | 2021-08-11 | Pivot Bio Inc | Composiciones agricolas que comprenden microbios remodelados de fijacion de nitrogeno. |
| BR112021003797A2 (pt) | 2018-08-29 | 2021-05-25 | Pioneer Hi-Bred International, Inc. | proteínas inseticidas e métodos para seu uso |
| EP3864157A4 (en) * | 2018-10-10 | 2022-11-02 | Pioneer Hi-Bred International, Inc. | INSECTICIDAL PROTEINS AND METHODS OF USE |
| EP3897104A4 (en) * | 2018-12-17 | 2022-09-07 | Syngenta Crop Protection AG | INSECTICIDAL PROTEINS |
| WO2021076346A1 (en) | 2019-10-18 | 2021-04-22 | Pioneer Hi-Bred International, Inc. | Maize event dp-202216-6 and dp-023211-2 stack |
| TW202142114A (zh) | 2020-02-04 | 2021-11-16 | 美商陶氏農業科學公司 | 具有殺有害生物效用之組成物及與其相關之方法 |
| EP4182466A2 (en) | 2020-07-14 | 2023-05-24 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| BR112023002603A2 (pt) | 2020-08-10 | 2023-04-04 | Pioneer Hi Bred Int | Elementos reguladores de plantas e métodos de uso dos mesmos |
| CN112480219B (zh) * | 2020-11-30 | 2021-10-12 | 东北农业大学 | Sip1Aa可溶性杀虫蛋白突变体 |
| CN112480220B (zh) * | 2020-12-02 | 2021-10-12 | 东北农业大学 | 苏云金芽胞杆菌Sip1Aa蛋白随机重组突变蛋白 |
| TW202345696A (zh) | 2022-05-18 | 2023-12-01 | 美商科迪華農業科技有限責任公司 | 具有殺有害生物效用之組成物及與其相關的方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR8600161A (pt) * | 1985-01-18 | 1986-09-23 | Plant Genetic Systems Nv | Gene quimerico,vetores de plasmidio hibrido,intermediario,processo para controlar insetos em agricultura ou horticultura,composicao inseticida,processo para transformar celulas de plantas para expressar uma toxina de polipeptideo produzida por bacillus thuringiensis,planta,semente de planta,cultura de celulas e plasmidio |
| US5608142A (en) * | 1986-12-03 | 1997-03-04 | Agracetus, Inc. | Insecticidal cotton plants |
| US5024837A (en) * | 1987-05-06 | 1991-06-18 | Donovan William P | Coleopteran active microorganisms, related insecticide compositions and methods for their production and use |
| GB8910624D0 (en) * | 1989-05-09 | 1989-06-21 | Ici Plc | Bacterial strains |
| US5308760A (en) * | 1992-01-10 | 1994-05-03 | Washington Research Foundation | Crystal proteins of Bacillus thuringiensis, genes encoding them, and host expressing them |
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- 2004-07-06 MX MXPA06000293A patent/MXPA06000293A/es unknown
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- 2004-07-06 RU RU2006103365/13A patent/RU2382822C2/ru not_active IP Right Cessation
- 2004-07-06 US US10/563,270 patent/US8796026B2/en active Active
- 2004-07-06 CN CN200480025352.7A patent/CN1849397B/zh active Active
- 2004-07-06 CA CA002531400A patent/CA2531400A1/en not_active Abandoned
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| CN1849397B (zh) | 2014-06-04 |
| RU2382822C2 (ru) | 2010-02-27 |
| EP1644480B1 (en) | 2013-06-05 |
| WO2005019414A2 (en) | 2005-03-03 |
| AU2004267355A1 (en) | 2005-03-03 |
| EP1644480A4 (en) | 2007-02-21 |
| BRPI0412390A (pt) | 2006-09-19 |
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