ZA200300827B - Method of producing functional protein domains. - Google Patents

Method of producing functional protein domains.

Info

Publication number
ZA200300827B
ZA200300827B ZA200300827A ZA200300827A ZA200300827B ZA 200300827 B ZA200300827 B ZA 200300827B ZA 200300827 A ZA200300827 A ZA 200300827A ZA 200300827 A ZA200300827 A ZA 200300827A ZA 200300827 B ZA200300827 B ZA 200300827B
Authority
ZA
South Africa
Prior art keywords
functional protein
protein domains
producing functional
producing
domains
Prior art date
Application number
ZA200300827A
Other languages
English (en)
Inventor
Giampiero De Luca
Luca Falciola
Original Assignee
Applied Research Systems
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applied Research Systems filed Critical Applied Research Systems
Publication of ZA200300827B publication Critical patent/ZA200300827B/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
ZA200300827A 2000-08-01 2003-01-30 Method of producing functional protein domains. ZA200300827B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB0018876.3A GB0018876D0 (en) 2000-08-01 2000-08-01 Method of producing polypeptides

Publications (1)

Publication Number Publication Date
ZA200300827B true ZA200300827B (en) 2004-02-25

Family

ID=9896767

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA200300827A ZA200300827B (en) 2000-08-01 2003-01-30 Method of producing functional protein domains.

Country Status (20)

Country Link
US (1) US8048645B2 (enExample)
EP (1) EP1305411B1 (enExample)
JP (1) JP5068916B2 (enExample)
CN (1) CN1468304B (enExample)
AR (1) AR031717A1 (enExample)
AT (1) ATE398174T1 (enExample)
AU (1) AU7573901A (enExample)
BR (1) BR0112979A (enExample)
CA (1) CA2415768C (enExample)
CY (1) CY1110431T1 (enExample)
DE (1) DE60134410D1 (enExample)
DK (1) DK1305411T3 (enExample)
ES (1) ES2305091T3 (enExample)
GB (1) GB0018876D0 (enExample)
IL (1) IL154212A0 (enExample)
NO (1) NO330897B1 (enExample)
PT (1) PT1305411E (enExample)
SI (1) SI1305411T1 (enExample)
WO (1) WO2002010372A1 (enExample)
ZA (1) ZA200300827B (enExample)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1572994T3 (da) * 2002-12-20 2007-05-29 Chromagenics Bv Midler og fremgangsmåder til fremstilling af en protein gennem chromatinåbnere, som kan göre chromatin mere tilgængeligt for transkriptionsfaktorer
AU2004245175C1 (en) * 2003-06-10 2010-03-18 Biogen Ma Inc. Improved secretion of neublastin
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
WO2011053957A2 (en) * 2009-11-02 2011-05-05 Gen9, Inc. Compositions and methods for the regulation of multiple genes of interest in a cell
CA2817697C (en) 2010-11-12 2021-11-16 Gen9, Inc. Methods and devices for nucleic acids synthesis
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
JP2014526899A (ja) 2011-08-26 2014-10-09 ジェン９・インコーポレイテッド 核酸の高忠実度アセンブリのための組成物および方法
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CA2871505C (en) 2012-04-24 2021-10-12 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
WO2014004393A1 (en) 2012-06-25 2014-01-03 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing
KR102160389B1 (ko) 2013-08-05 2020-09-28 트위스트 바이오사이언스 코포레이션 드 노보 합성된 유전자 라이브러리
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
CA2975855C (en) 2015-02-04 2025-09-23 Twist Bioscience Corporation SYNTHETIC GENE COMPOSITIONS AND ASSEMBLY METHODS
WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
KR20180050411A (ko) 2015-09-18 2018-05-14 트위스트 바이오사이언스 코포레이션 올리고핵산 변이체 라이브러리 및 그의 합성
WO2017053450A1 (en) 2015-09-22 2017-03-30 Twist Bioscience Corporation Flexible substrates for nucleic acid synthesis
EP3384077A4 (en) 2015-12-01 2019-05-08 Twist Bioscience Corporation FUNCTIONALIZED SURFACES AND MANUFACTURE THEREOF
WO2018038772A1 (en) 2016-08-22 2018-03-01 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
EP4664304A3 (en) 2016-09-21 2026-01-21 Atlas Data Storage, Inc. Nucleic acid based data storage
GB2573069A (en) 2016-12-16 2019-10-23 Twist Bioscience Corp Variant libraries of the immunological synapse and synthesis thereof
WO2018156792A1 (en) 2017-02-22 2018-08-30 Twist Bioscience Corporation Nucleic acid based data storage
CA3056388A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
IL271205B2 (en) 2017-06-12 2025-02-01 Twist Bioscience Corp Methods for assembling continuous nucleic acids
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
IL312616B2 (en) 2018-01-04 2025-08-01 Twist Bioscience Corp Dna-based digital information storage
EP3814497A4 (en) 2018-05-18 2022-03-02 Twist Bioscience Corporation POLYNUCLEOTIDES, REAGENTS AND METHODS FOR NUCLEIC ACID HYBRIDIZATION
EP3902816A4 (en) 2018-12-26 2022-09-14 Twist Bioscience Corporation HIGH-PRECISION DE NOVO POLYNUCLEOTIDE SYNTHESIS
KR20210144698A (ko) 2019-02-26 2021-11-30 트위스트 바이오사이언스 코포레이션 항체 최적화를 위한 변이 핵산 라이브러리
CA3131689A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for glp1 receptor
EP3987019A4 (en) 2019-06-21 2023-04-19 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
US12091777B2 (en) 2019-09-23 2024-09-17 Twist Bioscience Corporation Variant nucleic acid libraries for CRTH2
CN115023440B (zh) 2019-09-23 2025-09-12 特韦斯特生物科学公司 结合CD3ε的抗体
AU2020400030B2 (en) 2019-12-09 2026-05-21 Twist Bioscience Corporation Variant nucleic acid libraries for adenosine receptors
KR20230078719A (ko) 2020-09-28 2023-06-02 베스타론 코포레이션 해충 방제용 뮤-디구에톡신-Dc1a 변이형 폴리펩타이드
US20240374761A1 (en) 2021-05-05 2024-11-14 Radius Pharmaceuticals, Inc. Animal model having homologous recombination of mouse pth1 receptor
WO2023122805A1 (en) 2021-12-20 2023-06-29 Vestaron Corporation Sorbitol driven selection pressure method
WO2024026406A2 (en) 2022-07-29 2024-02-01 Vestaron Corporation Next Generation ACTX Peptides

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2646438B1 (fr) * 1989-03-20 2007-11-02 Pasteur Institut Procede de remplacement specifique d'une copie d'un gene present dans le genome receveur par l'integration d'un gene different de celui ou se fait l'integration
SG88714A1 (en) 1989-11-06 2002-05-21 Cell Genesys Inc Production of proteins using homologous recombination
US5272071A (en) 1989-12-22 1993-12-21 Applied Research Systems Ars Holding N.V. Method for the modification of the expression characteristics of an endogenous gene of a given cell line
DE779362T1 (de) * 1989-12-22 2001-04-05 Applied Research Systems Ars Holding N.V., Curacao DNS-Konstrukten zur Aktivierung und Veränderung der Expression von endogenen Genen
WO1993004169A1 (en) * 1991-08-20 1993-03-04 Genpharm International, Inc. Gene targeting in animal cells using isogenic dna constructs
US6063630A (en) * 1991-11-05 2000-05-16 Transkaryotic Therapies, Inc. Targeted introduction of DNA into primary or secondary cells and their use for gene therapy
US5968502A (en) 1991-11-05 1999-10-19 Transkaryotic Therapies, Inc. Protein production and protein delivery
US5641670A (en) 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery
US6270989B1 (en) * 1991-11-05 2001-08-07 Transkaryotic Therapies, Inc. Protein production and delivery
TW402639B (en) * 1992-12-03 2000-08-21 Transkaryotic Therapies Inc Protein production and protein delivery
CN1256986C (zh) * 1993-12-02 2006-05-24 转移染色体治疗公司 蛋白质的生产和蛋白质的输送
US5545817A (en) * 1994-03-11 1996-08-13 Calgene, Inc. Enhanced expression in a plant plastid
WO1997023244A1 (en) 1995-12-22 1997-07-03 Smithkline Beecham Corporation Use of antisene oligodeoxynucleotides to produce truncated proteins
DE69841723D1 (de) * 1997-09-26 2010-07-29 Abt Holding Co Expression von endogenen genen durch homologe rekombination eines vektorkonstruktes mit zellulärer dna
US7063960B2 (en) * 1997-12-12 2006-06-20 The Rockefeller University Protein belonging to the TNF superfamily involved in signal transduction, nucleic acids encoding same, and methods of use thereof
CA2323834C (en) 1998-03-27 2009-01-27 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
US6436707B1 (en) * 1998-03-27 2002-08-20 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
JP3688118B2 (ja) * 1998-05-22 2005-08-24 独立行政法人科学技術振興機構 遺伝子トラップ用ベクターと、このベクターを用いた遺伝子トラップ方法

Also Published As

Publication number Publication date
HK1059281A1 (en) 2004-06-25
JP2004504840A (ja) 2004-02-19
AR031717A1 (es) 2003-10-01
CN1468304B (zh) 2011-01-19
NO330897B1 (no) 2011-08-08
EP1305411A1 (en) 2003-05-02
JP5068916B2 (ja) 2012-11-07
CN1468304A (zh) 2004-01-14
US20040053362A1 (en) 2004-03-18
NO20030443L (no) 2003-03-25
GB0018876D0 (en) 2000-09-20
EP1305411B1 (en) 2008-06-11
CA2415768C (en) 2011-05-17
DK1305411T3 (da) 2008-09-01
SI1305411T1 (sl) 2008-10-31
CA2415768A1 (en) 2002-02-07
NO20030443D0 (no) 2003-01-28
CY1110431T1 (el) 2015-04-29
US8048645B2 (en) 2011-11-01
DE60134410D1 (de) 2008-07-24
ES2305091T3 (es) 2008-11-01
WO2002010372A1 (en) 2002-02-07
ATE398174T1 (de) 2008-07-15
AU7573901A (en) 2002-02-13
BR0112979A (pt) 2003-07-08
IL154212A0 (en) 2003-07-31
PT1305411E (pt) 2008-07-03
WO2002010372A8 (en) 2002-02-28

Similar Documents

Publication Publication Date Title
AU7573901A (en) Method of producing functional protein domains
AU2001280201A1 (en) Methods of synthesizing cell-free protein
GB0001704D0 (en) Protein
IL149029A0 (en) High mannose proteins and methods for producing the same
AU2000279641A1 (en) Method for producing protein hydrolysate
GB2366639B (en) Method of comparing parts
AU2002214286A1 (en) Method of profiling protein
GB0019375D0 (en) Method of polypeptide renaturation
MXPA03003347A (es) Procedimiento para purificacion de pravastatina.
ATE516267T1 (de) Vefahren zur herstellung von lactamen
GB0217404D0 (en) Combinatorial protein domains
PL346034A1 (en) Method of obtaining substituted banzoylcyanide aminohydrazones
ZA200210361B (en) Method of administering bishosphonates.
GB2384779B (en) Method for the production of tagged proteins
GB0024938D0 (en) Method of diagnosis
GB0009181D0 (en) Protein
AU8626801A (en) Method of forming complex
AU9227301A (en) Novel proteins, genes encoding them and method of using the same
GB0011269D0 (en) Protein over expression
AU2001286268A1 (en) Method of forming complex
ZA200107459B (en) Method of arranging information.
IL138141A0 (en) Method of doing business
GB0008549D0 (en) Protein
PL344649A1 (en) Method of obtaining multi-ester type plastifying agents
GB0019362D0 (en) Combinational protein Domains