ZA200102780B - Method for obtaining active β-NGF. - Google Patents
Method for obtaining active β-NGF. Download PDFInfo
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- ZA200102780B ZA200102780B ZA200102780A ZA200102780A ZA200102780B ZA 200102780 B ZA200102780 B ZA 200102780B ZA 200102780 A ZA200102780 A ZA 200102780A ZA 200102780 A ZA200102780 A ZA 200102780A ZA 200102780 B ZA200102780 B ZA 200102780B
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- South Africa
- Prior art keywords
- prongf
- ngf
- denaturing
- concentration
- protein
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- 201000005572 sensory peripheral neuropathy Diseases 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 239000011537 solubilization buffer Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 210000001913 submandibular gland Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000003774 sulfhydryl reagent Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000002889 sympathetic effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- 239000002753 trypsin inhibitor Substances 0.000 description 1
- 230000001810 trypsinlike Effects 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
- 239000011800 void material Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/48—Nerve growth factor [NGF]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/02—Drugs for disorders of the nervous system for peripheral neuropathies
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Neurology (AREA)
- Neurosurgery (AREA)
- Animal Behavior & Ethology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Molecular Biology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Hospice & Palliative Care (AREA)
- Psychiatry (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Steroid Compounds (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP98119077A EP0994188B1 (de) | 1998-10-09 | 1998-10-09 | Verfahren zur Gewinnung von aktivem Beta-NGF |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ZA200102780B true ZA200102780B (en) | 2002-07-04 |
Family
ID=8232766
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ZA200102780A ZA200102780B (en) | 1998-10-09 | 2001-04-04 | Method for obtaining active β-NGF. |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US8318671B1 (pt) |
| EP (1) | EP0994188B1 (pt) |
| JP (2) | JP4634611B2 (pt) |
| KR (1) | KR100593831B1 (pt) |
| AT (1) | ATE257514T1 (pt) |
| AU (1) | AU765810B2 (pt) |
| CA (1) | CA2346257C (pt) |
| DE (1) | DE59810555D1 (pt) |
| ES (1) | ES2213861T3 (pt) |
| FR (1) | FR17C0007I2 (pt) |
| NL (1) | NL300894I2 (pt) |
| WO (1) | WO2000022119A1 (pt) |
| ZA (1) | ZA200102780B (pt) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0994188B1 (de) | 1998-10-09 | 2004-01-07 | Scil proteins GmbH | Verfahren zur Gewinnung von aktivem Beta-NGF |
| US8066997B2 (en) | 2002-12-20 | 2011-11-29 | Anders Nykjaer | Modulation of activity of neurotrophins |
| RS60019B1 (sr) | 2006-12-21 | 2020-04-30 | H Lundbeck As | Modulacija aktivnosti proneurotrofina |
| HUE036265T2 (hu) | 2011-12-19 | 2018-06-28 | Wacker Chemie Ag | Új proNGF mutánsok és alkalmazásaik béta-NGF elõállításában |
| CN103880943A (zh) * | 2014-01-20 | 2014-06-25 | 厦门北大之路生物工程有限公司 | 一种rhNGF成熟肽的制备方法 |
| CN106519024A (zh) * | 2015-09-15 | 2017-03-22 | 三生国健药业（上海）股份有限公司 | 一种用于去除单克隆抗体中异构体的复性液及复性方法 |
| EP3406259A1 (en) | 2017-05-24 | 2018-11-28 | Dompé farmaceutici S.p.A. | Neurotrophins for use in the treatment of hearing loss |
| CN108467428A (zh) * | 2018-03-26 | 2018-08-31 | 江苏中新医药有限公司 | 一种清除rhNGF中N端截短及异常变异体的方法 |
| MA52351A (fr) | 2018-04-27 | 2021-05-26 | Chiesi Farm Spa | Production du facteur de croissance nerveuse (ngf) et de mutéines de celui-ci |
| JP2022502359A (ja) | 2018-09-17 | 2022-01-11 | キエージ・フアルマチエウテイチ・エツセ・ピ・ア | 皮膚科障害の処置のための薬剤 |
| CN110093394B (zh) * | 2019-05-10 | 2023-10-03 | 重庆科润生物医药研发有限公司 | 一种蛋白包涵体及重组人β-神经生长因子的制备方法 |
| DK4031117T3 (da) | 2019-09-17 | 2024-09-16 | Chiesi Farm S P A | NGF-mutant til anvendelse i behandling eller forebyggelse af oftalmiske lidelser |
| IL302317A (en) | 2020-10-28 | 2023-06-01 | Dompe Farm Spa | Pharmaceutical packaging including polypropylene containers and aqueous formulations of NGF packed in them |
| EP4039269A1 (en) | 2021-02-05 | 2022-08-10 | Dompe' Farmaceutici S.P.A. | Ngf isoform for use in the treatment of ocular pathologies |
| MX2023012006A (es) | 2021-04-13 | 2024-05-15 | Dompe Farm Spa | Tratamiento del dolor corneal neuropatico con factor de crecimiento nervioso (ngf). |
| EP4311554A1 (en) | 2022-07-29 | 2024-01-31 | Dompé farmaceutici S.p.a. | Combination for use in ophthalmology |
| EP4316505A1 (en) | 2022-08-05 | 2024-02-07 | Dompé farmaceutici S.p.a. | Intranasal administration of ngf for the treatment of sensorineural hearing loss |
| EP4342485A1 (en) | 2022-09-23 | 2024-03-27 | Dompe' Farmaceutici S.P.A. | Ngf for the treatment of spasticity |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5169762A (en) * | 1983-03-03 | 1992-12-08 | Genentech, Inc. | Human nerve growth factor by recombinant technology |
| US5453363A (en) | 1985-10-23 | 1995-09-26 | Boehringer Mannheim Gmbh | Process for the activation of t-PA or Ing after genetic expression in prokaryotes |
| US5683894A (en) | 1988-04-29 | 1997-11-04 | University Of California | Recombinant nerve growth factor |
| US5235043A (en) * | 1990-04-06 | 1993-08-10 | Synergen, Inc. | Production of biologically active, recombinant members of the ngf/bdnf family of neurotrophic proteins |
| US5606031A (en) | 1990-04-06 | 1997-02-25 | Lile; Jack | Production and purification of biologically active recombinant neurotrophic protein in bacteria |
| DE4139000A1 (de) * | 1991-11-27 | 1993-06-03 | Boehringer Mannheim Gmbh | Verfahren zur gentechnologischen herstellung von biologisch aktivem ss-ngf |
| DE4405179A1 (de) | 1994-02-18 | 1995-08-24 | Hoechst Ag | Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken |
| AU3564595A (en) * | 1994-09-12 | 1996-03-29 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Biologically active molecules derived from neurotrophins |
| US5935824A (en) * | 1996-01-31 | 1999-08-10 | Technologene, Inc. | Protein expression system |
| EP0904355B1 (de) | 1996-06-11 | 2004-03-03 | Boehringer Mannheim Gmbh | Verfahren zur aktivierung von denaturiertem protein |
| EP0994188B1 (de) | 1998-10-09 | 2004-01-07 | Scil proteins GmbH | Verfahren zur Gewinnung von aktivem Beta-NGF |
-
1998
- 1998-10-09 EP EP98119077A patent/EP0994188B1/de not_active Expired - Lifetime
- 1998-10-09 DE DE59810555T patent/DE59810555D1/de not_active Expired - Lifetime
- 1998-10-09 ES ES98119077T patent/ES2213861T3/es not_active Expired - Lifetime
- 1998-10-09 AT AT98119077T patent/ATE257514T1/de active
-
1999
- 1999-10-11 WO PCT/EP1999/007613 patent/WO2000022119A1/de active IP Right Grant
- 1999-10-11 KR KR1020017004512A patent/KR100593831B1/ko active IP Right Grant
- 1999-10-11 CA CA002346257A patent/CA2346257C/en not_active Expired - Lifetime
- 1999-10-11 JP JP2000576009A patent/JP4634611B2/ja not_active Expired - Lifetime
- 1999-10-11 US US09/807,096 patent/US8318671B1/en not_active Expired - Lifetime
- 1999-10-11 AU AU10348/00A patent/AU765810B2/en not_active Expired
-
2001
- 2001-04-04 ZA ZA200102780A patent/ZA200102780B/en unknown
-
2010
- 2010-04-22 US US12/765,229 patent/US8501439B2/en not_active Expired - Lifetime
- 2010-09-06 JP JP2010199387A patent/JP5275306B2/ja not_active Expired - Lifetime
-
2017
- 2017-09-27 FR FR17C0007C patent/FR17C0007I2/fr active Active
- 2017-09-28 NL NL300894C patent/NL300894I2/nl unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ES2213861T3 (es) | 2004-09-01 |
| KR100593831B1 (ko) | 2006-06-28 |
| BR9914393A (pt) | 2001-06-26 |
| US8501439B2 (en) | 2013-08-06 |
| CA2346257C (en) | 2005-03-01 |
| US8318671B1 (en) | 2012-11-27 |
| NL300894I2 (nl) | 2018-02-01 |
| JP2010280717A (ja) | 2010-12-16 |
| JP4634611B2 (ja) | 2011-02-16 |
| BR9914393B1 (pt) | 2021-06-22 |
| JP5275306B2 (ja) | 2013-08-28 |
| EP0994188A1 (de) | 2000-04-19 |
| CA2346257A1 (en) | 2000-04-20 |
| US20100203589A1 (en) | 2010-08-12 |
| KR20010083900A (ko) | 2001-09-03 |
| FR17C0007I1 (fr) | 2017-11-17 |
| JP2002527062A (ja) | 2002-08-27 |
| AU1034800A (en) | 2000-05-01 |
| FR17C0007I2 (fr) | 2018-11-09 |
| WO2000022119A1 (de) | 2000-04-20 |
| DE59810555D1 (de) | 2004-02-12 |
| ATE257514T1 (de) | 2004-01-15 |
| EP0994188B1 (de) | 2004-01-07 |
| AU765810B2 (en) | 2003-10-02 |
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