WO2024077469A1 - 一种冰叶日中花高效栽培的方法 - Google Patents

一种冰叶日中花高效栽培的方法 Download PDF

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WO2024077469A1
WO2024077469A1 PCT/CN2022/124549 CN2022124549W WO2024077469A1 WO 2024077469 A1 WO2024077469 A1 WO 2024077469A1 CN 2022124549 W CN2022124549 W CN 2022124549W WO 2024077469 A1 WO2024077469 A1 WO 2024077469A1
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seeds
cultivation
present
light source
led light
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PCT/CN2022/124549
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English (en)
French (fr)
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王向誉
乔鹏
顾寅钰
付娆
马兰
宋延静
田伟
张洪霞
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山东省蚕业研究所
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Priority to PCT/CN2022/124549 priority Critical patent/WO2024077469A1/zh
Publication of WO2024077469A1 publication Critical patent/WO2024077469A1/zh

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00—Soilless cultivation, e.g. hydroponics
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth

Definitions

  • the invention relates to the technical field of plant cultivation, in particular to a method for efficiently cultivating mesona glabra.
  • Mesembryanthemum crystallinum L. also known as ice flower, ice leaf daisy, African ice grass, is an annual or biennial herb belonging to the Aizoaceae family and the genus Mesembryanthemum. It is named for the transparent, reflective bumps on its leaves. Studies have shown that a layer of salt-controlling cells is attached to the surface of its leaves and stems, which has a very unique taste. It also has high nutritional value and health benefits, and is a functional vegetable.
  • Ice-leaf daylily is a shade-loving crop, and open-field cultivation is only suitable from February to May and from August to October each year. Hot summers and cold winters are not conducive to plant growth, and it requires cultivation under high saline-alkali conditions to bring out the characteristics and taste of the plant. However, in actual production, not all cultivation sites are suitable for the growth of ice-leaf daylily.
  • the cultivation methods of ice-leaf daylily include direct seeding and plug tray seedling cultivation. The germination rate of direct seeding is extremely low, wasting a lot of seeds.
  • plug tray seedling cultivation solves the problem of seed waste caused by direct seeding, there is still the problem of uneven seedling emergence. Therefore, it is particularly important to propose a simple and efficient ice-leaf daylily cultivation method.
  • the present invention proposes a method for efficiently cultivating Zinnia glaciosa, by screening seeds, using LED light source to promote rapid germination of seeds during the cultivation process, and applying a culture solution specially used for the growth of Zinnia glaciosa, so that the Zinnia glaciosa can maintain its original special quality, improve the germination rate of seeds, and ensure uniform germination and high survival rate.
  • the cultivation method is simple and efficient.
  • the present invention provides the following solutions:
  • the present invention provides a method for efficiently cultivating Melancholia glaucosa, comprising the following steps:
  • Seed screening Place the seeds in water and stir, let it stand, take the seeds at the bottom and pass them through a 2 mm sieve, and dry them;
  • Seed pretreatment Soak the selected seeds in clean water at 20-23°C and illuminate them with an LED light source until the seeds germinate and grow 2-3 leaves;
  • Substrate preparation Fill the holes of the seedling tray with peat soil, then move the seedlings into the holes, one seedling per hole, fix them with a sponge, and then add culture solution at 25°C and culture under shade;
  • the seeds can be harvested after 50-60 days of cultivation.
  • the stirring speed is 80r/min and the stirring time is 6min.
  • the standing time is 10 minutes.
  • the illumination intensity of the LED light source is 80 ⁇ mol ⁇ m -2 ⁇ s -1 , and the illumination time is 6 h/d.
  • the LED light source is red light of 660nm and blue light of 450nm.
  • the culture solution supply cycle is 4 min/5 h (ie, it is supplied once every 5 h, and each supply lasts 4 min), and the dripping speed is 0.05 mL/min.
  • the culture solution includes P: 1.5mmol/L, S: 1.5mmol/L, K: 6.0mmol/L, Mg: 1.4mmol/L, Fe: 35 ⁇ mol/L, Mn: 10 ⁇ mol/L, Cu: 0.5 ⁇ mol/L, NO 3 -N: 10.5mmol/L, NH 4 -N: 2mmol/L, and B: 30 ⁇ mol/L.
  • the cultivation method of the ice leaf sunflower of the present invention can improve the germination rate of seeds, and the germination is uniform and the survival rate is high.
  • the cultivation method is simple and efficient, which can break through the limitations of the current ice leaf sunflower cultivation conditions and realize the large-scale production of ice leaf sunflower.
  • the present invention removes impurities from the ice leaf sunflower by screening the seeds, and simultaneously screens the seeds with large particle size and good quality for cultivation. During the cultivation process, LED light source illumination is used to promote rapid germination of the seeds.
  • the culture solution of the present invention is a special formula suitable for the growth of ice leaf sunflower, and the culture environment is also the most suitable temperature for the growth of ice leaf sunflower. The ice leaf sunflower cultivated under this culture condition can maintain its original special quality.
  • the present invention provides a method for efficiently cultivating Melancholia glaucosa, comprising the following steps:
  • Seed screening Place the seeds in water and stir, let it stand, take the seeds at the bottom and pass them through a 2 mm sieve, and dry them;
  • Seed pretreatment Soak the selected seeds in clean water at 20-23°C and illuminate them with an LED light source until the seeds germinate and grow 2-3 leaves;
  • Substrate preparation Fill the holes of the seedling tray with peat soil, then move the seedlings into the holes, one seedling per hole, fix them with a sponge, and then add culture solution at 25°C and culture under shade;
  • the seeds can be harvested after 50-60 days of cultivation.
  • the culture medium used in the embodiment of the present invention includes P: 1.5mmol/L, S: 1.5mmol/L, K: 6.0mmol/L, Mg: 1.4mmol/L, Fe: 35 ⁇ mol/L, Mn: 10 ⁇ mol/L, Cu: 0.5 ⁇ mol/L, NO3 -N: 10.5mmol/L, NH4 -N: 2mmol/L, B: 30 ⁇ mol/L.
  • the preparation process is not limited, and the prepared culture medium can be purchased as long as it meets the above conditions.
  • the environmental temperature for growing the ice leaf mesona in the embodiment of the present invention is 22°C. All materials used in the embodiment of the present invention are purchased from the market. All equipment used in the embodiment of the present invention are existing equipment and can be purchased from the market.
  • a method for efficiently cultivating Melancholia glaucosa comprising the following steps:
  • Seed screening Place the seeds in water, stir at 80 r/min for 6 min, let stand for 10 min, take the seeds at the bottom and pass them through a 2 mm sieve, and dry them naturally;
  • Seed pretreatment The selected seeds were soaked in clean water at 20°C and illuminated with an LED light source (red light 660nm, blue light 450nm) until the seeds germinated and grew three leaves.
  • the intensity of the LED light source was 80 ⁇ mol ⁇ m -2 ⁇ s -1 and the illumination time was 6h/d.
  • Substrate preparation fill the holes of the seedling tray with peat soil, then move the seedlings into the holes, one seedling per hole, fix them with a sponge, plant 50 trees, and then add culture solution (including P: 1.5 mmol/L, S: 1.5 mmol/L, K: 6.0 mmol/L, Mg: 1.4 mmol/L, Fe: 35 ⁇ mol/L, Mn: 10 ⁇ mol/L, Cu: 0.5 ⁇ mol/L, NO 3 -N: 10.5 mmol/L, NH 4 -N: 2 mmol/L, B: 30 ⁇ mol/L) at 25°C at a rate of 0.05 mL/min, with a cycle of 4 min/5 h, use a shade net, and perform shade cultivation at a shade degree of 32%;
  • a method for efficiently cultivating Melancholia glaucosa comprising the following steps:
  • Seed screening Place the seeds in water, stir at 80 r/min for 6 min, let stand for 10 min, take the seeds at the bottom and pass them through a 2 mm sieve, and dry them naturally;
  • Seed pretreatment The selected seeds were soaked in clean water at 23°C and illuminated with an LED light source (red light 660nm, blue light 450nm) until the seeds germinated and grew two leaves.
  • the intensity of the LED light source was 80 ⁇ mol ⁇ m -2 ⁇ s -1 and the illumination time was 6h/d.
  • Substrate preparation fill the holes of the seedling tray with peat soil, then move the seedlings into the holes, one seedling per hole, fix them with a sponge, plant 50 trees, and then add culture solution (including P: 1.5 mmol/L, S: 1.5 mmol/L, K: 6.0 mmol/L, Mg: 1.4 mmol/L, Fe: 35 ⁇ mol/L, Mn: 10 ⁇ mol/L, Cu: 0.5 ⁇ mol/L, NO 3 -N: 10.5 mmol/L, NH 4 -N: 2 mmol/L, B: 30 ⁇ mol/L) at 25°C at a rate of 0.05 mL/min, with a cycle of 4 min/5 h, use a shade net, and perform shade cultivation at a shade degree of 32%;
  • a method for efficiently cultivating Melancholia glaucosa comprising the following steps:
  • Seed screening Place the seeds in water, stir at 80 r/min for 6 min, let stand for 10 min, take the seeds at the bottom and pass them through a 2 mm sieve, and dry them naturally;
  • Seed pretreatment The selected seeds were soaked in clean water at 22°C and illuminated with an LED light source (red light 660nm, blue light 450nm) until the seeds germinated and grew three leaves.
  • the intensity of the LED light source was 80 ⁇ mol ⁇ m -2 ⁇ s -1 and the illumination time was 6h/d.
  • Substrate preparation fill the holes of the seedling tray with peat soil, then move the seedlings into the holes, one seedling per hole, fix them with a sponge, plant 50 trees, and then add culture solution (including P: 1.5 mmol/L, S: 1.5 mmol/L, K: 6.0 mmol/L, Mg: 1.4 mmol/L, Fe: 35 ⁇ mol/L, Mn: 10 ⁇ mol/L, Cu: 0.5 ⁇ mol/L, NO 3 -N: 10.5 mmol/L, NH 4 -N: 2 mmol/L, B: 30 ⁇ mol/L) at 25°C at a rate of 0.05 mL/min, with a cycle of 4 min/5 h, use a shade net, and perform shade cultivation at a shade degree of 32%;
  • a method for efficiently cultivating Melancholia glaucosa comprising the following steps:
  • Seed screening Place the seeds in water, stir at 80 r/min for 6 min, let stand for 10 min, take the seeds at the bottom and pass them through a 2 mm sieve, and dry them naturally;
  • Seed pretreatment The selected seeds were soaked in clean water at 23°C and illuminated with an LED light source (red light 660nm, blue light 450nm) until the seeds germinated and grew three leaves.
  • the intensity of the LED light source was 80 ⁇ mol ⁇ m -2 ⁇ s -1 and the illumination time was 6h/d.
  • Substrate preparation fill the holes of the seedling tray with peat soil, then move the seedlings into the holes, one seedling per hole, fix them with a sponge, plant 50 trees, and then add culture solution (including P: 1.5 mmol/L, S: 1.5 mmol/L, K: 6.0 mmol/L, Mg: 1.4 mmol/L, Fe: 35 ⁇ mol/L, Mn: 10 ⁇ mol/L, Cu: 0.5 ⁇ mol/L, NO 3 -N: 10.5 mmol/L, NH 4 -N: 2 mmol/L, B: 30 ⁇ mol/L) at 25°C at a rate of 0.05 mL/min, with a cycle of 4 min/5 h, use a shade net, and perform shade cultivation at a shade degree of 32%;
  • step (2) is omitted.
  • step (1) is omitted.
  • Example 2 Same as Example 1, except that the culture solution is replaced by water.
  • step (2) the illumination time of the LED light source is 10 h/d.
  • Comparative Example 1 when the LED light source is omitted, the germination rate is low and the germination time is long. In Comparative Example 5, when the LED light source illumination time is changed, the germination rate of the seeds is also affected. In Comparative Example 2, the seed screening step is omitted. Although the late stage has a strong growth, the germination rate is also low due to the low germplasm. In Comparative Example 3, when no culture solution is added, the growth of the ice leaf daylily in the late stage is obviously not as good as that of other groups, and the seedlings are easy to fall over. Comparative Example 4 changes the environmental temperature of the seed germination soaking, which also reduces the germination rate of the seeds. It can be seen from Examples 1-4 of the present invention that the cultivation method of the present invention has a high germination rate, which can reach 100%, a high seedling survival rate, and is more suitable for the growth of ice leaf daylily.

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Abstract

本发明公开了一种冰叶日中花高效栽培的方法，属于植物栽培技术领域。本发明冰叶日中花高效栽培的方法，包括以下步骤：将种子置于水中搅拌，静置，取底部种子过2mm筛，晾干；将筛选后的种子用20-23℃的清水浸泡，并用LED光源光照，至种子萌发长出2-3叶；将草炭土填充于育苗盘的穴孔内，之后将幼苗移入穴孔中，每孔1苗，用海绵固定，然后在25℃下滴加培养液，遮阴培养；培养50-60天即可采收。本发明冰叶日中花的栽培方法可以提高种子的出苗率，出苗整齐且成活率高，培养方法简单、高效，可突破目前冰叶日中花栽培地条件的限制，实现冰叶日中花的大规模生产。

Description

一种冰叶日中花高效栽培的方法 技术领域
本发明涉及植物栽培技术领域，特别是涉及一种冰叶日中花高效栽培的方法。
背景技术
冰叶日中花(Mesembryanthemum crystallinum L.)，别名：冰花、冰叶菊、非洲冰草，属番杏科、日中花属一年生或二年生草本，因其叶上有透明、反光的隆起而得名，且有研究表明其叶子部分同茎部的表层附着着一层控盐细胞，具有非常独特的口感，同时具有较高的营养价值和保健作用，属一种功能性蔬菜。
冰叶日中花为喜阴凉作物，露地栽培只适合在每年2至5月和8至10月进行，炎热夏季及寒冷冬季都不利于植株生长，并且其要求在高盐碱条件下栽培，才能发挥植物的特性和口味，但实际生产中不是所有的栽培地都适合冰叶日中花的生长。目前，冰叶日中花的栽培方式有种子直播和穴盘育苗，种子直播发芽率极低，浪费大量种子，穴盘育苗虽然解决了种子直播造成浪费种子的问题，但是仍存在出苗不整齐的问题。因此，提出一种简单且高效的冰叶日中花栽培方法尤为重要。
发明内容
为了解决上述技术问题，本发明提出了一种冰叶日中花高效栽培的方法，通过对种子进行筛选，培养过程中，采用LED光源光照促进种子快速萌发，施加冰叶日中花生长专用的培养液，从而使冰叶日中花可保持其原有特殊品质，提高种子的出苗率，出苗整齐且成活率高，培养方法简单、 高效。
为实现上述目的，本发明提供了如下方案：
本发明提供了一种冰叶日中花高效栽培的方法，包括以下步骤：
(1)种子的筛选：将种子置于水中搅拌，静置，取底部种子过2mm筛，晾干；
(2)种子预处理：将筛选后的种子用20-23℃的清水浸泡，并用LED光源光照，至种子萌发长出2-3叶；
(3)基质准备：将草炭土填充于育苗盘的穴孔内，之后将幼苗移入穴孔中，每孔1苗，用海绵固定，然后在25℃下滴加培养液，遮阴培养；
(4)培养50-60天即可采收。
进一步地，所述搅拌的转速为80r/min，搅拌时间为6min。
进一步地，所述静置的时间为10min。
进一步地，所述LED光源光照的强度为80μmol·m -2·s -1，光照时间为6h/d。
进一步地，所述LED光源为红光660nm、蓝光450nm。
进一步地，所述培养液供应周期为4min/5h(即每5h供应一次，一次供应4min)，滴加速度为0.05mL/min。
进一步地，所述培养液包括P:1.5mmol/L，S:1.5mmol/L，K:6.0mmol/L，Mg:1.4mmol/L，Fe:35μmol/L，Mn:10μmol/L，Cu:0.5μmol/L，NO 3-N:10.5mmol/L，NH 4-N:2mmol/L，B:30μmol/L。
进一步地，遮阴培养的遮阴度为32％。
本发明公开了以下技术效果：
(1)本发明冰叶日中花的栽培方法可以提高种子的出苗率，出苗整齐且成活率高，培养方法简单、高效，可突破目前冰叶日中花栽培地条件的限制，实现冰叶日中花的大规模生产。
(2)本发明通过对种子进行筛选，除去冰叶日中花中的杂质，同时筛选粒径大、质量优的种子进行培养，培养过程中，采用LED光源光照促进种子快速萌发，另外，本发明的培养液是适宜冰叶日中花生长的专用配方，培养环境也是冰叶日中花生长的最适温度，在此培养条件下栽培得到的冰叶日中花可保持其原有特殊品质。
具体实施方式
现详细说明本发明的多种示例性实施方式，该详细说明不应认为是对本发明的限制，而应理解为是对本发明的某些方面、特性和实施方案的更详细的描述。
应理解本发明中所述的术语仅仅是为描述特别的实施方式，并非用于限制本发明。另外，对于本发明中的数值范围，应理解为还具体公开了该范围的上限和下限之间的每个中间值。在任何陈述值或陈述范围内的中间值以及任何其他陈述值或在所述范围内的中间值之间的每个较小的范围也包括在本发明内。这些较小范围的上限和下限可独立地包括或排除在范围内。
除非另有说明，否则本文使用的所有技术和科学术语具有本发明所述领域的常规技术人员通常理解的相同含义。虽然本发明仅描述了优选的方法和材料，但是在本发明的实施或测试中也可以使用与本文所述相似或等同的任何方法和材料。本说明书中提到的所有文献通过引用并入，用以公 开和描述与所述文献相关的方法和/或材料。在与任何并入的文献冲突时，以本说明书的内容为准。
在不背离本发明的范围或精神的情况下，可对本发明说明书的具体实施方式做多种改进和变化，这对本领域技术人员而言是显而易见的。由本发明的说明书得到的其他实施方式对技术人员而言是显而易见得的。本发明说明书和实施例仅是示例性的。
关于本文中所使用的“包含”、“包括”、“具有”、“含有”等等，均为开放性的用语，即意指包含但不限于。
本发明提供了一种冰叶日中花高效栽培的方法，包括以下步骤：
(1)种子的筛选：将种子置于水中搅拌，静置，取底部种子过2mm筛，晾干；
(2)种子预处理：将筛选后的种子用20-23℃的清水浸泡，并用LED光源光照，至种子萌发长出2-3叶；
(3)基质准备：将草炭土填充于育苗盘的穴孔内，之后将幼苗移入穴孔中，每孔1苗，用海绵固定，然后在25℃下滴加培养液，遮阴培养；
(4)培养50-60天即可采收。
本发明实施例中所用培养液包括P:1.5mmol/L，S:1.5mmol/L，K:6.0mmol/L，Mg:1.4mmol/L，Fe:35μmol/L，Mn:10μmol/L，Cu:0.5μmol/L，NO 3-N:10.5mmol/L，NH 4-N:2mmol/L，B:30μmol/L，配制过程没有限制，配制的培养液能购买满足上述条件即可。
本发明实施例中冰叶日中花栽培的环境温度为22℃。本发明实施例中所用各材料均为通过市售购买得到。本发明实施例中使用的设备均为现有 设备，可市场购得。
下面结合实施例对本方明做进一步的说明，但不局限于此：
实施例1
一种冰叶日中花高效栽培的方法，包括以下步骤：
(1)种子的筛选：将种子置于水中，在80r/min下搅拌6min，静置10min，取底部种子过2mm筛，自然晾干；
(2)种子预处理：将筛选后的种子用20℃的清水浸泡，并用LED光源(红光660nm、蓝光450nm)光照，至种子萌发长出3叶，其中LED光源光照的强度为80μmol·m -2·s -1，光照时间为6h/d；
(3)基质准备：将草炭土填充于育苗盘的穴孔内，之后将幼苗移入穴孔中，每孔1苗，用海绵固定，种植50棵，然后在25℃下按照0.05mL/min滴加培养液(包括P:1.5mmol/L，S:1.5mmol/L，K:6.0mmol/L，Mg:1.4mmol/L，Fe:35μmol/L，Mn:10μmol/L，Cu:0.5μmol/L，NO 3-N:10.5mmol/L，NH 4-N:2mmol/L，B:30μmol/L)，周期为4min/5h，使用遮阴网，在遮阴度为32％下进行遮阴培养；
(4)培养60天采收。
实施例2
一种冰叶日中花高效栽培的方法，包括以下步骤：
(1)种子的筛选：将种子置于水中，在80r/min下搅拌6min，静置10min，取底部种子过2mm筛，自然晾干；
(2)种子预处理：将筛选后的种子用23℃的清水浸泡，并用LED光源(红光660nm、蓝光450nm)光照，至种子萌发长出2叶，其中LED光 源光照的强度为80μmol·m -2·s -1，光照时间为6h/d；
(3)基质准备：将草炭土填充于育苗盘的穴孔内，之后将幼苗移入穴孔中，每孔1苗，用海绵固定，种植50棵，然后在25℃下按照0.05mL/min滴加培养液(包括P:1.5mmol/L，S:1.5mmol/L，K:6.0mmol/L，Mg:1.4mmol/L，Fe:35μmol/L，Mn:10μmol/L，Cu:0.5μmol/L，NO 3-N:10.5mmol/L，NH 4-N:2mmol/L，B:30μmol/L)，周期为4min/5h，使用遮阴网，在遮阴度为32％下进行遮阴培养；
(4)培养50天采收。
实施例3
一种冰叶日中花高效栽培的方法，包括以下步骤：
(1)种子的筛选：将种子置于水中，在80r/min下搅拌6min，静置10min，取底部种子过2mm筛，自然晾干；
(2)种子预处理：将筛选后的种子用22℃的清水浸泡，并用LED光源(红光660nm、蓝光450nm)光照，至种子萌发长出3叶，其中LED光源光照的强度为80μmol·m -2·s -1，光照时间为6h/d；
(3)基质准备：将草炭土填充于育苗盘的穴孔内，之后将幼苗移入穴孔中，每孔1苗，用海绵固定，种植50棵，然后在25℃下按照0.05mL/min滴加培养液(包括P:1.5mmol/L，S:1.5mmol/L，K:6.0mmol/L，Mg:1.4mmol/L，Fe:35μmol/L，Mn:10μmol/L，Cu:0.5μmol/L，NO 3-N:10.5mmol/L，NH 4-N:2mmol/L，B:30μmol/L)，周期为4min/5h，使用遮阴网，在遮阴度为32％下进行遮阴培养；
(4)培养60天采收。
实施例4
一种冰叶日中花高效栽培的方法，包括以下步骤：
(1)种子的筛选：将种子置于水中，在80r/min下搅拌6min，静置10min，取底部种子过2mm筛，自然晾干；
(2)种子预处理：将筛选后的种子用23℃的清水浸泡，并用LED光源(红光660nm、蓝光450nm)光照，至种子萌发长出3叶，其中LED光源光照的强度为80μmol·m -2·s -1，光照时间为6h/d；
(3)基质准备：将草炭土填充于育苗盘的穴孔内，之后将幼苗移入穴孔中，每孔1苗，用海绵固定，种植50棵，然后在25℃下按照0.05mL/min滴加培养液(包括P:1.5mmol/L，S:1.5mmol/L，K:6.0mmol/L，Mg:1.4mmol/L，Fe:35μmol/L，Mn:10μmol/L，Cu:0.5μmol/L，NO 3-N:10.5mmol/L，NH 4-N:2mmol/L，B:30μmol/L)，周期为4min/5h，使用遮阴网，在遮阴度为32％下进行遮阴培养；
(4)培养60天采收。
对比例1
同实施例1，区别仅在于，省去步骤(2)中用LED光源光照的步骤。
对比例2
同实施例1，区别仅在于，省略步骤(1)。
对比例3
同实施例1，区别仅在于，将培养液等质量替换为水。
对比例4
同实施例1，区别仅在于，步骤(2)中清水的温度为26℃。
对比例5
同实施例1，区别仅在于，步骤(2)中，LED光源的光照时间为10h/d。
实验例
冰叶日中花栽培期间，记录实施例1-4与对比例1-5的幼苗的出苗率、出苗时间、后期小苗长势、是否倒伏，出苗是指冰叶日中花种子露白，结果见表1。
表1实施例与对比例长势对比
  出苗率 出苗时间(天) 后期长势 是否倒伏
实施例1 100％ 2 强 否
实施例2 96％ 2 强 否
实施例3 96％ 2 强 否
实施例4 98％ 2 强 否
对比例1 80％ 6 弱 否
对比例2 70％ 4 强 否
对比例3 90％ 2 弱 是
对比例4 74％ 5 强 否
对比例5 64％ 4 强 否
由表1数据可以看出，对比例1在省略LED光源光照的情况下，出苗率较低，并且出苗时间较长，对比例5在改变LED光源光照的时间的情况下，同样对种子的出苗率产生了影响，对比例2省略了对种子筛选的步骤，虽然后期具有较强的长势，但是由于种质较低，出苗率也较低，对比例3在没有添加培养液的情况下，冰叶日中花后期的长势明显不如其它组，且幼苗易倒伏，对比例4改变了种子萌发浸泡的环境温度，同样也降低了种子的出芽率。由本发明实施例1-4可以看出，本发明的栽培方法出苗率高，可以达到100％，幼苗成活率高，更适宜冰叶日中花的生长。
以上所述的实施例仅是对本发明的优选方式进行描述，并非对本发明的范围进行限定，在不脱离本发明设计精神的前提下，本领域普通技术人员对本发明的技术方案做出的各种变形和改进，均应落入本发明权利要求书确定的保护范围内。

Claims (8)

  1. 一种冰叶日中花高效栽培的方法，其特征在于，包括以下步骤：
    (1)种子的筛选：将种子置于水中搅拌，静置，取底部种子过2mm筛，晾干；
    (2)种子预处理：将筛选后的种子用20-23℃的清水浸泡，并用LED光源光照，至种子萌发长出2-3叶；
    (3)基质准备：将草炭土填充于育苗盘的穴孔内，之后将幼苗移入穴孔中，每孔1苗，用海绵固定，然后在25℃下滴加培养液，遮阴培养；
    (4)培养50-60天即可采收。
  2. 根据权利要求1所述的冰叶日中花高效栽培的方法，其特征在于，所述搅拌的转速为80r/min，搅拌时间为6min。
  3. 根据权利要求1所述的冰叶日中花高效栽培的方法，其特征在于，所述静置的时间为10min。
  4. 根据权利要求1所述的冰叶日中花高效栽培的方法，其特征在于，所述LED光源光照的强度为80μmol·m -2·s -1，光照时间为6h/d。
  5. 根据权利要求4所述的冰叶日中花高效栽培的方法，其特征在于，所述LED光源为红光660nm、蓝光450nm。
  6. 根据权利要求1所述的冰叶日中花高效栽培的方法，其特征在于，所述培养液供应周期为4min/5h，滴加速度为0.05mL/min。
  7. 根据权利要求1所述的冰叶日中花高效栽培的方法，其特征在于，所述培养液包括P:1.5mmol/L，S:1.5mmol/L，K:6.0mmol/L，Mg:1.4mmol/L，Fe:35μmol/L，Mn:10μmol/L，Cu:0.5μmol/L，NO 3-N:10.5mmol/L，NH 4-N:2mmol/L，B:30μmol/L。
  8. 根据权利要求1所述的冰叶日中花高效栽培的方法，其特征在于，遮阴培养的遮阴度为32％。
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