WO2024049041A1 - Procédé de préparation d'un engrais fonctionnel à l'aide de dioxyde de carbone - Google Patents

Procédé de préparation d'un engrais fonctionnel à l'aide de dioxyde de carbone Download PDF

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Publication number
WO2024049041A1
WO2024049041A1 PCT/KR2023/011633 KR2023011633W WO2024049041A1 WO 2024049041 A1 WO2024049041 A1 WO 2024049041A1 KR 2023011633 W KR2023011633 W KR 2023011633W WO 2024049041 A1 WO2024049041 A1 WO 2024049041A1
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Prior art keywords
present
acid
functional fertilizer
carbon dioxide
fermentation
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PCT/KR2023/011633
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English (en)
Korean (ko)
Inventor
신현용
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주식회사 카본엔네이처
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Publication of WO2024049041A1 publication Critical patent/WO2024049041A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor

Definitions

  • the present invention relates to a method for manufacturing functional fertilizers using organic acids produced from carbon dioxide and functional fertilizers manufactured using the same.
  • CCU Carbon Capture and Utilization
  • Microalgae that perform photosynthesis are used to fix carbon, and by multiplying them in large quantities, not only various biofuels, but also fatty acids, animal feed, pigments, and pharmaceutical substances are made based on the increased microalgae. .
  • CCU technology is attracting attention as one of the key technologies for realizing a zero-carbon society.
  • 'organic acid fermentation by microorganisms' refers to a process of producing various organic acids by incompletely oxidizing carbon compounds through a fermentation reaction by microorganisms.
  • Various substances such as acetic acid, succinic acid, etc. can be produced through microbial fermentation reactions.
  • the present inventors have made extensive research efforts to establish an organic acid production method with excellent carbon capture and conversion efficiency and carbon reduction effect and to develop a method of utilizing it.
  • the present invention was completed by confirming the functionality (improvement of productivity and prevention of cold damage) of a fertilizer containing organic acids produced through microbial ( Actinobacillus succinogenes ) fermentation.
  • the purpose of the present invention is to provide a method for producing functional fertilizer.
  • Another object of the present invention is to provide a functional fertilizer containing organic acids.
  • the present inventors have made extensive research efforts to establish an organic acid production method with excellent carbon capture and conversion efficiency and carbon reduction effect and to develop a method of utilizing it. As a result, the present inventors made extensive research efforts to establish an organic acid production method with excellent carbon capture and conversion efficiency and a carbon reduction effect and to develop a method of utilizing it. As a result, the functionality of fertilizer containing organic acids produced through microbial ( Actinobacillus succinogenes ) fermentation (improvement of productivity/marketability, prevention of cold damage, promotion of root growth and germination, etc.) was confirmed.
  • the present invention relates to carbon dioxide capture and use, a method for manufacturing functional fertilizer, and functional fertilizer manufactured using the same.
  • the present invention provides a method for producing functional fertilizer, comprising a fermentation step by Actinobacillus succinogenes (ATCC 55618).
  • the term “fermentation” refers to a process in which microorganisms obtain energy by decomposing organic nutrients (such as glucose) in an anaerobic environment.
  • the fermentation step by the microorganism ( Actinobacillus succinogenes ) of the present invention is performed in a bioreactor, and the inside of the bioreactor contains microorganisms and a culture medium for performing organic acid fermentation.
  • the method according to the present invention is suitable for producing various organic acids, including but not limited to succinic acid, formic acid, acetic acid, lactic acid, and valeric acid.
  • Organic acids selected from the group consisting of acid, malic acid, citric acid, and mixtures thereof can be produced.
  • fermentation microorganisms used in the method according to the present invention include single microorganisms (Actinobacillus succinogenes) or mixed microorganisms that produce organic acids.
  • the Actinobacillus succinogene strain (ATCC 55618) is characterized by containing the 16s rRNA base sequence of SEQ ID NO: 1.
  • the microorganism may be added in an amount of 0.001 to 0.2 parts by weight based on 100 parts by weight of the culture medium.
  • the amount of microorganisms added is less than 0.001 parts by weight, the fermentation time becomes too long, and if the amount of microorganisms added exceeds 0.2 parts by weight, it is difficult to obtain a mature fermentation product.
  • microorganisms that perform fermentation and culture medium are mixed.
  • the culture medium of the present invention includes the microorganism (strain) as an active ingredient, a culture thereof, a culture containing the bacterial cells in addition to the cells isolated and/or purified, an extract of the bacterial cells, a culture supernatant, a concentrate, and a concentrate thereof. It may include water, dried matter, and, if necessary, diluted liquid, diluted matter, etc., culture medium, and all forms obtained by processing the culture.
  • the culture medium used in the method according to the present invention may contain a carbon source, an alkaline base, and components (strain growth components) essential for the growth of microorganisms (strains).
  • the carbon source is a sugar and includes monosaccharides, disaccharides, polysaccharides, or mixtures thereof, such as glucose, fructose, sucrose, galactose, mannose, xylose, arabinose, sugar cane, Molasses, starch hydrolyzate, etc. may be mentioned, but are not limited thereto.
  • the alkaline base is a weak base containing an alkali metal from group 1 of the periodic table that maintains a pH suitable for the growth of microorganisms for fermentation of carbon sources, and examples include, but are not limited to, KOH.
  • the content ratio of the carbon source and alkaline base in the culture medium supplied during fermentation can be arbitrarily adjusted by the user to suit the characteristics of fermentation, taking into account the solubility of the base, the rate of organic acid production, etc.
  • the content ratio of the carbon source and alkaline base can be arbitrarily changed during the fermentation process under the premise that the sterilization state of the supplied carbon source and alkaline base is maintained.
  • essential components for the growth of the strain include nitrogen sources, vitamins, inorganic salts, and/or carbon source decomposition enzymes such as invertase in addition to carbon source substrates, but are limited thereto. It doesn't work. A person skilled in the art can easily determine components essential for growth depending on the strain based on known techniques.
  • the nitrogen source includes organic nitrogen sources such as yeast extract, corn steep liquor (CSL), peptone, broth, malt extract, and soybean meal; and inorganic nitrogen sources such as urea, ammonium sulfate, ammonium chloride, ammonium phosphate, ammonium carbonate, and ammonium nitrate; or a combination thereof, but is not limited thereto.
  • organic nitrogen sources such as yeast extract, corn steep liquor (CSL), peptone, broth, malt extract, and soybean meal
  • inorganic nitrogen sources such as urea, ammonium sulfate, ammonium chloride, ammonium phosphate, ammonium carbonate, and ammonium nitrate; or a combination thereof, but is not limited thereto.
  • the method may further include the step of injecting carbon dioxide (CO 2 ) and/or carbonate.
  • the carbonate may include calcium carbonate (CaCO 3 ), potassium carbonate (K 2 CO 3 ), and sodium hydrogen carbonate (NaHCO 3 ), but is not limited thereto.
  • carbon dioxide gas and/or carbonate are injected into the bioreactor using a peristaltic pump, and a separate stirring means (motor) is further provided to facilitate dissolution of carbon dioxide gas into the culture medium. .
  • the stirring speed may be 200 to 500 rpm.
  • carbon dioxide gas is first injected into the bioreactor containing the culture medium using a metering pump, etc., and the injected carbon dioxide gas is mixed with the culture medium within the bioreactor.
  • the carbon dioxide gas may be carbon dioxide gas generated from a carbon dioxide emission source such as a general thermal power plant or biomass. Therefore, when using the present invention, it is possible to effectively recycle carbon dioxide generated during biomass processing to lower the pH of the fermentation broth.
  • the fermentation step is performed by fermenting the culture medium inoculated with microorganisms at a temperature of 30 to 45 °C, preferably 37 °C, at a speed of 100 to 1,000 rpm, preferably 200 rpm for 12 to 48 hours, preferably 36 hours. This may be accomplished.
  • the stirring speed is less than 100, and/or the fermentation time is less than 12 hours, the production yield of organic acids is very low, and if the temperature of the fermentation step exceeds 45°C, or the stirring speed is If the speed exceeds 1,000 rpm and/or the fermentation time exceeds 48 hours, the composition and production of organic acids may decrease.
  • the method of the present invention is economical because it does not require separate processes such as purification.
  • the present invention provides succinic acid, formic acid, acetic acid, lactic acid, valeric acid, malic acid and Provided is a functional fertilizer containing one or more organic acids selected from the group consisting of citric acid.
  • the organic acid may be produced by Actinobacillus succinogenes (ATCC 55618) fermentation.
  • the functional fertilizer of the present invention may include a culture medium containing an organic acid produced by the above-described organic acid production method for producing functional fertilizer.
  • the functional fertilizer of the present invention may, if necessary, further contain trace elements generally related to plant growth and fruit production, such as potassium, calcium, magnesium, sulfur, and boric acid.
  • the functional fertilizer of the present invention has effects such as suppressing cold damage, promoting root growth, and promoting germination.
  • the description of the organic acid contained in the functional fertilizer of the present invention is the same or similar to the above-described description of the organic acid production method for producing the functional fertilizer of the present invention, so it will be omitted.
  • the present invention relates to a functional fertilizer containing organic acids produced by carbon dioxide capture and conversion technology.
  • the functional fertilizer according to the present invention solves crop necrosis caused by environmental pollution caused by existing chemical fertilizers or chemical toxicity of crops. It has the effect of improving resistance to external stresses such as cold damage.
  • Figure 1 is a schematic diagram showing a method for manufacturing functional fertilizer according to an embodiment of the present invention.
  • Figure 2 is a diagram showing temperature changes during an experiment conducted to confirm the functionality of fertilizer manufactured according to an embodiment of the present invention.
  • Figure 3 shows the results of confirming the effect of improving the productivity and marketability of chives (control group (left), experimental group (right)) by functional fertilizer manufactured according to an embodiment of the present invention.
  • Figure 4 shows the results of confirming the effect of suppressing cold damage and improving marketability of peppers (control group (left), experimental group (right)) by the functional fertilizer prepared according to an embodiment of the present invention.
  • Actinobacillus succinogenes was cultured (shaking incubator) in an Erlenmeyer flask at 37°C for 16 hours using BHI (Brain Heart Infusion) medium (Kisan Biotech, Korea).
  • the cultured Actinobacillus succinogenes were fermented in a 3-liter bioreactor (Applikon Schiedam, Netherlands).
  • the medium was KH 2 PO 4 (3g/L), 3% refined sugar (white sugar) or molasses (Molasses) was used as a carbon source, and 4% CSL (corn steep liquid) or 2% yeast extract was used as a nitrogen source. did.
  • Actinobacillus succinogene culture medium 10 mL was injected into 1 L of medium (culture medium), and fermented with stirring at 37°C for 36 hours at a speed of 200 rpm to prepare functional fertilizer using organic acids and microorganisms.
  • carbon dioxide was injected (500 ml/hr) using a tubing pump, and 8M KOH was used to adjust pH to maintain pH 6.8.
  • the concentration (production amount) of the organic acid was measured at 210 nm using HPLC (Shimadzu, Japan), an Aminex HPX-87H column (Bio-Rad, USA), and a UV meter (Shimadzu). was measured.
  • HPLC HPLC
  • the HPLC mobile phase used 0.005N sulfuric acid, and the speed of the mobile phase was set at 0.6ml/min. The measurement results are shown in Table 1 below.
  • Carbon dioxide captured and organic acid production (based on 24 hours) g/L ⁇ day Carbon dioxide (CO 2 ) capture amount 0.67 Succinic acid production 0.58 Formic acid production 0.30 Acetic acid production 0.24
  • the present invention relates to a method for manufacturing functional fertilizers using organic acids produced from carbon dioxide and functional fertilizers manufactured using the same.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Fertilizers (AREA)

Abstract

La présente invention concerne un engrais fonctionnel comprenant un acide organique produit par une technique de capture et de conversion de dioxyde de carbone. L'engrais fonctionnel selon la présente invention résout le problème de la pollution environnementale provoquée par les engrais chimiques existants ou la nécrose des cultures en raison de la toxicité chimique dans les cultures et améliore la résistance à un stress externe tel qu'un endommagement par le froid.
PCT/KR2023/011633 2022-08-31 2023-08-08 Procédé de préparation d'un engrais fonctionnel à l'aide de dioxyde de carbone WO2024049041A1 (fr)

Applications Claiming Priority (2)

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KR10-2022-0110330 2022-08-31
KR1020220110330A KR20240032226A (ko) 2022-08-31 2022-08-31 이산화탄소를 이용한 기능성 비료의 제조방법

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WO2024049041A1 true WO2024049041A1 (fr) 2024-03-07

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100830858B1 (ko) * 2007-12-12 2008-05-22 금산군 인삼사포닌 발효액을 이용한 엽채류 재배방법
CN102146422A (zh) * 2011-01-24 2011-08-10 南京工业大学 一种丁二酸的发酵生产工艺
KR20140042566A (ko) * 2012-09-28 2014-04-07 롯데케미칼 주식회사 미세조류를 질소원으로 이용한 숙신산의 생산 방법
KR20160004110A (ko) * 2014-07-02 2016-01-12 한국과학기술연구원 이산화탄소 유래 숙신산 생산을 위한 균주 및 이를 이용한 이산화탄소 유래 숙신산 생산 방법

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101843586B1 (ko) 2012-05-23 2018-03-30 에스케이이노베이션 주식회사 탄소원 기질과 염기의 유가식 공급에 의한 유기산 제조 방법

Patent Citations (4)

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Publication number Priority date Publication date Assignee Title
KR100830858B1 (ko) * 2007-12-12 2008-05-22 금산군 인삼사포닌 발효액을 이용한 엽채류 재배방법
CN102146422A (zh) * 2011-01-24 2011-08-10 南京工业大学 一种丁二酸的发酵生产工艺
KR20140042566A (ko) * 2012-09-28 2014-04-07 롯데케미칼 주식회사 미세조류를 질소원으로 이용한 숙신산의 생산 방법
KR20160004110A (ko) * 2014-07-02 2016-01-12 한국과학기술연구원 이산화탄소 유래 숙신산 생산을 위한 균주 및 이를 이용한 이산화탄소 유래 숙신산 생산 방법

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WEI ZOU;LI-WEN ZHU;HONG-MEI LI;YA-JIE TANG: "Significance of CO2 donor on the production of succinic acid by Actinobacillus succinogenes ATCC 55618", MICROBIAL CELL FACTORIES, SPRINGER, vol. 10, no. 1, 31 October 2011 (2011-10-31), pages 87, XP021093672, ISSN: 1475-2859, DOI: 10.1186/1475-2859-10-87 *

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