WO2023283969A1 - Boîtier de carte de détection d'acide nucléique et son procédé d'assemblage - Google Patents

Boîtier de carte de détection d'acide nucléique et son procédé d'assemblage Download PDF

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Publication number
WO2023283969A1
WO2023283969A1 PCT/CN2021/107090 CN2021107090W WO2023283969A1 WO 2023283969 A1 WO2023283969 A1 WO 2023283969A1 CN 2021107090 W CN2021107090 W CN 2021107090W WO 2023283969 A1 WO2023283969 A1 WO 2023283969A1
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
box body
cover plate
acid detection
channel
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PCT/CN2021/107090
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English (en)
Chinese (zh)
Inventor
肖博
王东伟
陈铖
曾玉祥
朱芸增
康建成
孟祥睿
陆沙
闫露露
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北京乐普智慧医疗科技有限公司
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Publication of WO2023283969A1 publication Critical patent/WO2023283969A1/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Definitions

  • the invention relates to nucleic acid extraction technology, in particular to a nucleic acid detection cartridge and a corresponding assembly process.
  • molecular diagnostic POCT As a popular direction for the development of molecular diagnostics in the future, molecular diagnostic POCT combines the characteristics of compactness, flexibility, convenience, speed, and accuracy. It has more and wider application scenarios and fields than traditional molecular diagnostics. The reason for the layout of this field; however, to realize the true sense of "sample in, result out" molecular diagnostic POCT needs to have the following basic elements: the design of a fully enclosed cartridge, the integration of extraction + amplification + detection, operation Minimalist, low-cost, multiplex PCR technology, reagent room temperature technology, rapid extraction and purification technology, portable molecular diagnostic POCT instrument. At present, there are no molecular POCT products that truly realize the above basic elements on the market.
  • the present invention provides a nucleic acid detection cartridge and its assembly process, which is an extraordinarly designed fully enclosed cartridge structure, low in cost, extremely simple in operation, and can realize the integration of extraction, amplification and detection change.
  • the present invention provides the following technical solutions:
  • a nucleic acid detection cartridge comprising a sampling tube and a box body, the box body is provided with several cavities for containing materials used for nucleic acid extraction and several fluid channels communicated with the cavities, and the sampling tube is connected to one of the cavities body, the fluid channels can be selectively communicated with each other.
  • the box body includes a box body and an upper cover plate covering the top of the box body, the upper cover plate is provided with a feed hole corresponding to the cavity, and the upper surface of the upper cover plate is covered with a top sealing film for sealing.
  • the feeding hole corresponding to the cavity containing the volatile substance is configured with a sealing cork.
  • the fluid channel is divided into a first-layer fluid channel and a second-layer fluid channel, and the lower end surface of the box body is provided with a plurality of first micro-pipes, and the first micro-pipes communicate with the cavity through holes;
  • the box body also includes a lower cover plate covering the bottom of the box body, the lower end surface of the lower cover plate is provided with a plurality of second micro-pipes, the lower end surface of the lower cover plate covers the bottom sealing film for sealing,
  • the second micro-pipe cooperates with the bottom sealing film to form a second-layer fluid channel
  • the first micro-pipe cooperates with the upper end surface of the lower cover to form a first-layer fluid channel.
  • an amplification detection area is also set on the lower cover, the amplification detection area includes an amplification detection array and a detection micropipe communicating with the amplification detection array, and the upper end of the amplification detection area covers the detection area.
  • the area sealing film is used for sealing.
  • the second micropipe is provided with several fluid state cavities
  • the box body is provided with fluid observation windows corresponding to the fluid state cavities.
  • the nucleic acid detection cartridge further includes a rotation mechanism, and the rotation mechanism controls the selective communication between the fluid channels through rotation.
  • the rotating mechanism includes a soft cushion and a rotating valve connected in sequence.
  • the lower end surface of the soft cushion is provided with a number of positioning holes, and the positioning holes cooperate with a number of positioning columns arranged on the box body to fix the rotating valve.
  • the cushion is connected to the fluid channel through a number of through holes
  • the lower end surface of the rotary valve is provided with a liquid flow channel and a gas flow channel, and the liquid flow channel can selectively communicate with the fluid through the through holes
  • the gas channel is selectively connected to the fluid channel through the through hole.
  • the rotating mechanism also includes a limit ring and a limit wall, the lower end surface of the limit ring is set as a bonding area and a smooth area, and the smooth area is pressed against the edge of the rotary valve for sealing.
  • the bonding area is connected to the limiting wall, and the limiting ring cooperates with the limiting wall to form a rotation space.
  • the box body is also provided with a gas inlet and outlet channel, the gas inlet and outlet channel can be selectively connected to the gas flow channel or cavity, the lower port of the gas inlet and outlet channel is covered with a waterproof and breathable film, the The gas inlet and outlet channels control the flow direction of substances used in nucleic acid extraction by providing gas power.
  • the length of the box body is 50mm-70mm, and/or the width is 20mm-40mm, and/or the height is 15mm-30mm.
  • the present invention also provides a nucleic acid detection cartridge assembly process, comprising the following steps:
  • Step 1 The lower cover plate is bonded to the box body to form a fluid channel
  • Step 2 The box body is bonded with the upper cover to form a complete cavity
  • Step 3 Assemble the rotating mechanism, put the soft cushion into the limit wall, fix the rotating mechanism, ensure that the through holes on the soft cushion correspond to the corresponding through holes on the upper end surface of the box body, and then install the rotating valve above the cushion;
  • Step 4 adding liquid, adding substances used for nucleic acid extraction into the cavity one by one, and then sealing;
  • Step 5 Add primers to the amplification detection array and seal it.
  • the assembly process of said step 1 includes:
  • the bottom sealing film is bonded to the lower cover to form a second layer of fluid channels
  • the lower cover plate is bonded to the box body to form the first layer of fluid channels.
  • the invention realizes the molecular diagnosis POCT in the true sense of "sample in, result out”. It adopts the design of a fully enclosed cartridge with a compact structure and greatly reduces the cost; all the reagents used are pre-installed in the detection cartridge, no need Added separately; realize the integration of extraction + amplification + detection, and at the same time have low requirements for supporting instruments, and only need simple operation to realize the whole process of molecular diagnosis.
  • the present invention also adopts multiple PCR technology, reagent room temperature technology, rapid extraction and purification technology, and can realize the whole-process extraction and detection of nucleic acid without a professional molecular biology laboratory. Departments, as small as community and township health centers, are especially suitable for environments where conventional instruments are not suitable for work.
  • Fig. 1 is a schematic diagram of the overall structure of a nucleic acid detection cartridge of the present invention
  • Fig. 2 is an exploded view from the upper perspective of a nucleic acid detection cartridge of the present invention
  • Fig. 3 is an exploded view of a nucleic acid detection cartridge of the present invention from a lower perspective;
  • Fig. 4 is a schematic view of the front structure of the box body of the present invention.
  • Fig. 5 is a schematic diagram of the reverse structure of the box body of the present invention.
  • Fig. 6 is the bottom view of the box body of the present invention.
  • Figure 7 is a top view of the box body of the present invention.
  • Fig. 8 is a schematic view of the front structure of the upper cover plate of the present invention.
  • Fig. 9 is a schematic diagram of the reverse structure of the upper cover plate of the present invention.
  • Fig. 10 is a schematic view of the front structure of the lower cover plate of the present invention.
  • Fig. 11 is a schematic diagram of the reverse structure of the lower cover plate of the present invention.
  • Fig. 12 is a schematic view of the front structure of the rotary valve of the present invention.
  • Fig. 13 is a schematic diagram of the reverse structure of the rotary valve of the present invention.
  • Fig. 14 is a schematic view of the front structure of the cushion of the present invention.
  • Fig. 15 is a schematic diagram of the back structure of the cushion of the present invention.
  • Fig. 16 is a schematic view of the front structure of the limiting ring of the present invention.
  • Fig. 17 is a schematic diagram of the reverse structure of the limiting ring of the present invention.
  • Figure 18 is a schematic diagram of the use of the rotary valve in the initial position of the present invention.
  • Fig. 19 is a schematic diagram of the use of the rotary valve in the second step of the use method of the present invention.
  • a nucleic acid detection cartridge which includes a sampling tube 1 and a box body 2. Inside the box body 2 are provided several cavities for containing substances used for nucleic acid extraction and several fluid channels communicating with the cavities , the sampling tube 1 is connected to one of the cavities, and several fluid channels can be selectively communicated with each other.
  • the nucleic acid detection cartridge of the present invention is very delicate in design, flexible in use, and greatly reduces various costs.
  • the length range (L) of the box body 2 is 50mm-70mm, preferably 70mm;
  • the width range ( W) is 20mm-40mm, preferably 33mm;
  • height range (H) is 15mm-30mm, preferably 20mm.
  • the box body 2 is a generally square overall structure, which is composed of several internal cavities, micro-pipes, via holes, etc., and at least a magnetic bead cavity 101, an extraction cavity 102, a waste liquid cavity 103, and a sample cavity are arranged inside.
  • 104 first cleaning chamber 105 , second cleaning chamber 106 , elution chamber 107 , reagent chamber 108 , spare chamber 109 , installation hole 1012 , wherein the sampling tube 1 is connected to the sample chamber 104 .
  • the box body 2 includes a box body 22 and an upper cover plate 21 covering the top of the box body 22, several cavities described above are arranged inside the box body 22, and the lower end surface of the box body 22 ( The reverse side) is provided with a number of first micro-pipes 221 of different sizes, and the first micro-pipes 221 communicate with the corresponding cavities sequentially through a number of via holes, so that each group of cavities and the corresponding first micro-pipes 221 are independent of each other. No interference, forming an independent channel;
  • the upper cover plate 21 is a flat plate provided with a number of feeding holes 211 corresponding to the cavity, and the upper end surface of the upper cover plate 21 is covered with a top sealing film 212 for sealing.
  • the feed hole 211 corresponding to the cavity containing the volatile liquid is configured with a sealing cork 213 before covering the top sealing film 212 to prevent the liquid from volatilizing, such as the magnetic bead cavity 101, the cleaning cavity 105 and The second chamber 106 is cleaned.
  • the box body 2 also includes a lower cover plate 23 covering the lower side of the box body 22.
  • the lower cover plate 23 is a flat plate, divided into two sides, the front side is upward, and an amplification detection area is provided, including the amplification detection area.
  • the detection array 121 (through the upper and lower sides), the detection micropipe 122 communicated with the amplification detection array, and the upper end surface of the amplification detection area covers the detection area sealing film 123 for sealing.
  • the reverse side Downward is the reverse side, which is provided with a number of second micro-pipes 231 of different sizes, wherein the front and back sides pass through the second micro-pipes 231 through a number of via holes, and communicate with the detection micro-pipes 122, so that each group of second micro-pipes 231 They are independent of each other and do not interfere with each other, forming an independent channel.
  • the lower end surface (reverse surface) of the lower cover plate 23 covers the bottom sealing film 232 for sealing the second micropipe 231 and the amplification detection area.
  • the fluid channel is divided into a first-layer fluid channel and a second-layer fluid channel
  • the second micro-pipe 231 cooperates with the bottom sealing film 232 to form the second-layer fluid channel
  • the upper end surface (front side) of the first micro-pipe 221 and the lower cover plate 23 ) cooperate to form a first-layer fluid channel
  • the first-layer fluid channel and the second-layer fluid channel cooperate to form a channel that can communicate between cavities.
  • the second micropipe 231 is provided with several fluid state cavities 2311
  • the box body 22 is provided with fluid observation windows 2312 corresponding to the fluid state cavities 2311, so as to dynamically observe the fluid state during the reaction.
  • the nucleic acid detection cartridge also includes a rotating mechanism 3, which is composed of a limit ring 31, a rotary valve 32, a cushion 33, and a limit wall 34.
  • the limit ring 31 and the limit wall 34 enclose a rotation space.
  • the soft pad 33 adopts a smooth and elastic material, such as elastomer materials such as styrene, styrene-isoprene-styrene block copolymer, polypropylene, thermoplastic polyurethane, etc. production.
  • a plurality of positioning holes 331 are provided on the lower end surface, and the positioning holes 331 cooperate with a plurality of positioning columns 332 provided on the box body 22 to fix the rotating mechanism 3 .
  • the soft pad 33 is also provided with a number of through holes that penetrate up and down, and the positions of the through holes are in one-to-one correspondence with the through holes on the corresponding positions of the box body 22, so as to realize the through holes.
  • the rotary valve 32 is a whole and is the only moving part in the rotary mechanism 3.
  • the lower end surface of the rotary valve 32 is provided with two long grooves 321 and short grooves 322 of different lengths.
  • the rotary valve 32 can be rotated so that the long groove 321 cooperates with the through hole on the cushion 33 to form a liquid channel, and the liquid channel can be selectively connected to each cavity; similarly, the rotary valve 32 can also be rotated to make the short
  • the groove 322 cooperates with the through hole on the cushion 33 to form a gas channel, and the gas channel can also be selectively connected to each cavity.
  • the lower end surface of the limiting ring 31 is set as a bonding area 311 and a smooth area 312, the bonding area 311 and the upper surface of the limiting wall 34 are completely fixed using a bonding process, and the smooth area 312 will be pressed down
  • Rotating the valve 32 forces the lower surface of the rotating valve 32 to press down on the cushion 33 to deform and form a certain pre-tightening force to play a sealing role.
  • the box body 22 is also provided with a gas inlet and outlet channel, including the air hole A1010, the air hole B1011 and the waste liquid chamber air outlet C1013, the air hole A1010 and the air hole B1011 run through the upper cover plate 21 and the top sealing film 212, and the waste liquid chamber air outlet C1013 is connected waste chamber.
  • a gas inlet and outlet channel including the air hole A1010, the air hole B1011 and the waste liquid chamber air outlet C1013, the air hole A1010 and the air hole B1011 run through the upper cover plate 21 and the top sealing film 212, and the waste liquid chamber air outlet C1013 is connected waste chamber.
  • the air hole A1010 or the air hole B1011 can be connected to the gas flow channel, and the air hole A1010 or the air hole B1011 can also be connected to the extraction chamber or the reagent chamber; and gas outlet, so as to provide gas power to control the flow direction of the substances used for nucleic acid extraction in each chamber.
  • the lower port of the gas inlet and outlet channel that is, the position of the lower end surface of the corresponding box body 22 is covered with a waterproof and breathable membrane 1014, which is used to isolate the internal and external environment of the detection cartridge, and has the function of blocking liquid and ventilation, ensuring the integrity of the detection cartridge.
  • Organic or harmful substances such as liquids and aerosols, as well as interfering substances in the external environment of the detection cartridge cannot enter and exit, avoiding pollution and interference.
  • the invention also discloses an assembly process of a nucleic acid detection cartridge, comprising the following steps:
  • Step 1 The bottom sealing film 232 is bonded to the lower cover plate 23 to form a second layer of fluid channels;
  • Step 2 Cover the waterproof and breathable membrane 1014 to the corresponding gas inlet and outlet channel positions on the lower end surface of the box body 22;
  • Step 3 The lower cover plate 23 is bonded to the lower end surface of the box body 22 to form a first-layer fluid channel;
  • Step 4 The box body 22 is bonded with the upper cover 21 to form a complete cavity
  • Step 5 Assemble the rotating mechanism 3:
  • First soft pad 33 is packed in the spacer wall 34, and then the positioning column 332 on the box body 22 cooperates with the positioning hole 331 on the soft pad 33 to connect and fix the position, and is close to the bottom surface to ensure that the via hole on the soft pad 33 is in line with the bottom surface.
  • the via holes on the upper end surface of the box body 22 in the limiting wall 34 correspond one by one;
  • the bonding process is used to completely fix, and at the same time, the smooth area 312 on the lower end surface of the limit ring 31 presses down on the rotary valve 32, forcing the lower surface of the rotary valve 32 to press down on the cushion 33 to produce deformation, forming a certain pre-tightening force, and playing a sealing role;
  • Step 6 Add liquid, add the substances used for nucleic acid extraction one by one through the feeding hole 211 of the upper cover plate 21, seal the feeding hole 211 with a sealing plug 213 for the volatile liquid, and then use the top sealing film 212 to bond with the upper cover plate 21 sealing;
  • Step 7 Add reagents to the reagent chamber, and seal it with the reagent chamber cover 214;
  • Step 8 Add primers to the amplification detection array, and use the detection area sealing film 123 to bond and seal.
  • the bonding described in the above steps refers to the fixed bonding of two same or different materials into one, such as pressure-sensitive adhesives, photosensitive adhesives, heat-sensitive adhesives, double-sided tape, thermal bonding, ultrasonic bonding, etc. Bonding, laser bonding, bump-to-groove coupling, etc.
  • the hard material and film material used in the detection cartridge can be made of polymer materials such as polypropylene, cycloolefin copolymer, cycloolefin polymer, polymethyl methacrylate, polystyrene or polycarbonate.
  • the method of using the nucleic acid detection cartridge in the present invention is as follows:
  • Step 1 Rotate the rotary valve 32, the extraction chamber and the magnetic bead chamber are connected through the fluid channel, the gas outlet from the air hole A1010, the air intake from the air hole B1011, the liquid in the magnetic bead chamber enters the extraction chamber, and the external magnet absorbs the magnetic beads after mixing;
  • Step 2 Rotate the rotary valve 32, the extraction chamber and the waste liquid chamber are communicated through the fluid channel, the air intake through the air hole A1010, the air out through the waste liquid chamber outlet C1013, and the waste liquid in the extraction chamber is discharged into the waste liquid chamber;
  • Step 3 Rotate the rotary valve 32, the extraction chamber and the sample chamber are connected through the fluid channel, the gas outlet from the air hole A1010, the air intake from the air hole B1011, the sample in the sample chamber enters the extraction chamber, the sample is mixed with the magnetic beads, and the magnetic beads absorb the nucleic acid in the sample After that, the external magnet absorbs the magnetic beads;
  • Step 4 Repeat Step 2;
  • Step 5 Rotate the rotary valve 32, the extraction chamber and the first cleaning chamber are connected through the fluid channel, the gas outlet from the air hole A1010, the air intake from the air hole B1011, the cleaning solution in the first cleaning chamber enters the extraction chamber, and after the nucleic acid adsorbed by the magnetic beads is cleaned, External magnet absorbs magnetic beads;
  • Step 6 Repeat Step 2;
  • Step 7 Rotate the rotary valve 32, the extraction chamber and the second cleaning chamber are connected through the fluid channel, the gas outlet of the air hole A1010, the air intake of the air hole B1011, the cleaning liquid in the second cleaning chamber enters the extraction chamber, and after the nucleic acid adsorbed by the magnetic beads is cleaned,
  • the external magnet absorbs the magnetic beads
  • Step 8 Repeat step 2;
  • Step 9 Rotate the rotary valve 32, the extraction chamber and the elution chamber are connected through the fluid channel, the gas outlet of the air hole A1010, the air intake of the air hole B1011, the eluent in the elution chamber enters the extraction chamber, the eluent is mixed with the magnetic beads, and the After the target nucleic acid substance adsorbed by the magnetic beads is eluted, the external magnet absorbs the magnetic beads;
  • Step 10 Rotate the rotary valve 32, the extraction chamber and the reagent chamber are connected through the fluid channel, the air is fed into the air hole A1010, and the air is discharged from the air hole B1011, and the eluent mixed with the target nucleic acid substance enters the reagent chamber and mixes with the freeze-dried reagent in the reagent chamber ; After the mixing is completed, air is released from the air hole A1010 and air is taken in from the air hole B1011, the external magnet remains in the adsorption state, and the mixed reagent in the reagent chamber enters the extraction chamber;
  • Step 11 Rotate the rotary valve 32, the extraction chamber and the amplification detection array are connected through the fluid channel, the air is fed into the air hole A1010, and the air is discharged from the air hole B1011, and the mixed reagent solution in the extraction chamber enters the amplification detection array;
  • Step 12 Rotate the rotary valve 32 so that the amplification detection area is completely closed, and start nucleic acid amplification and detection.

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Abstract

Boîtier de carte de détection d'acide nucléique, comprenant un tube de prélèvement (1) et un corps de boîtier (2). Le corps de boîtier (2) comprend un corps principal de boîtier (22), et un plateau de couvercle supérieur (21) et un plateau de couvercle inférieur (23), qui recouvrent respectivement une partie supérieure et une partie inférieure du corps principal de boîtier, plusieurs cavités contenant des substances utilisées pour l'extraction d'acide nucléique étant prévues dans le corps principal de boîtier (22), et le tube de prélèvement (1) étant en communication avec l'une des cavités ; et le plateau de couvercle supérieur (21) est pourvu d'un trou d'alimentation correspondant, le plateau de couvercle inférieur (23) et le corps principal de boîtier (22) sont joints pour former des canaux fluidiques, qui communiquent respectivement avec les cavités, et les canaux fluidiques se connectent sélectivement les uns aux autres. Une structure de boîtier de carte de type entièrement fermé et de conception fine est utilisée ; son coût est faible, son fonctionnement est extrêmement simple, l'extraction, l'amplification et la détection peuvent être intégrées, et la structure est adaptée à divers environnements, par exemple, aussi grand qu'un service d'urgence d'un hôpital tertiaire ou aussi petit qu'un centre de santé communautaire et de canton, et est particulièrement adaptée aux environnements où les instruments conventionnels ne sont pas adaptés pour travailler à l'heure actuelle.
PCT/CN2021/107090 2021-07-14 2021-07-19 Boîtier de carte de détection d'acide nucléique et son procédé d'assemblage WO2023283969A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202110792346.XA CN113308369A (zh) 2021-07-14 2021-07-14 一种核酸检测卡盒及其组装工艺
CN202110792346.X 2021-07-14

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Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010012612A1 (en) * 1999-05-28 2001-08-09 Cepheid Method for analyzing a fluid sample
CN1898560A (zh) * 2003-12-23 2007-01-17 法斯特拉克公司 现场即时诊断平台
US20080026451A1 (en) * 2006-06-15 2008-01-31 Braman Jeffrey C System for isolating biomolecules from a sample
US20100003683A1 (en) * 2006-07-14 2010-01-07 Roche Molecular Systems, Inc Disposable Device for Analyzing a Liquid Sample Containing a Nucleic Acid With a Nucleic Acid Amplification Apparatus
CN103370617A (zh) * 2010-10-01 2013-10-23 牛津纳米孔技术有限公司 利用纳米孔的生物化学分析设备
US20150353919A1 (en) * 2014-06-04 2015-12-10 Lucigen Corporation Sample collection and analysis devices
US20160375438A1 (en) * 2013-11-29 2016-12-29 Genewave Microfluidic cartridge for molecular diagnosis, docking station using a microfluidic cartridge, and process for analyzing a biological sample
CN107096580A (zh) * 2017-06-09 2017-08-29 北京百康芯生物科技有限公司 一种具有旋转阀结构的微流控芯片
CN107129930A (zh) * 2017-06-09 2017-09-05 北京百康芯生物科技有限公司 一种全集成核酸检测微流控芯片及其使用方法
CN110769934A (zh) * 2017-02-28 2020-02-07 美艾利尔圣地亚哥公司 微流体装置和相关方法
CN211288851U (zh) * 2019-12-05 2020-08-18 广州万孚生物技术股份有限公司 试剂卡及多通阀结构
CN112063512A (zh) * 2020-09-01 2020-12-11 北京乐普智慧医疗科技有限公司 一种包含提取扩增检测全流程的分子诊断卡及其使用工艺方法

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010012612A1 (en) * 1999-05-28 2001-08-09 Cepheid Method for analyzing a fluid sample
CN1898560A (zh) * 2003-12-23 2007-01-17 法斯特拉克公司 现场即时诊断平台
US20080026451A1 (en) * 2006-06-15 2008-01-31 Braman Jeffrey C System for isolating biomolecules from a sample
US20100003683A1 (en) * 2006-07-14 2010-01-07 Roche Molecular Systems, Inc Disposable Device for Analyzing a Liquid Sample Containing a Nucleic Acid With a Nucleic Acid Amplification Apparatus
CN103370617A (zh) * 2010-10-01 2013-10-23 牛津纳米孔技术有限公司 利用纳米孔的生物化学分析设备
US20160375438A1 (en) * 2013-11-29 2016-12-29 Genewave Microfluidic cartridge for molecular diagnosis, docking station using a microfluidic cartridge, and process for analyzing a biological sample
US20150353919A1 (en) * 2014-06-04 2015-12-10 Lucigen Corporation Sample collection and analysis devices
CN110769934A (zh) * 2017-02-28 2020-02-07 美艾利尔圣地亚哥公司 微流体装置和相关方法
CN107096580A (zh) * 2017-06-09 2017-08-29 北京百康芯生物科技有限公司 一种具有旋转阀结构的微流控芯片
CN107129930A (zh) * 2017-06-09 2017-09-05 北京百康芯生物科技有限公司 一种全集成核酸检测微流控芯片及其使用方法
CN211288851U (zh) * 2019-12-05 2020-08-18 广州万孚生物技术股份有限公司 试剂卡及多通阀结构
CN112063512A (zh) * 2020-09-01 2020-12-11 北京乐普智慧医疗科技有限公司 一种包含提取扩增检测全流程的分子诊断卡及其使用工艺方法

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