WO2023193376A1 - Trousse de dosage d'immunoabsoption par enzyme lié (test elisa) de lectine de pinellia ternata et application - Google Patents
Trousse de dosage d'immunoabsoption par enzyme lié (test elisa) de lectine de pinellia ternata et application Download PDFInfo
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- WO2023193376A1 WO2023193376A1 PCT/CN2022/109669 CN2022109669W WO2023193376A1 WO 2023193376 A1 WO2023193376 A1 WO 2023193376A1 CN 2022109669 W CN2022109669 W CN 2022109669W WO 2023193376 A1 WO2023193376 A1 WO 2023193376A1
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- 238000002965 ELISA Methods 0.000 title abstract description 7
- 108010068736 Pinellia ternata lectin Proteins 0.000 title abstract 5
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract description 41
- 108090000623 proteins and genes Proteins 0.000 claims description 12
- 241001522129 Pinellia Species 0.000 claims description 11
- 108090001090 Lectins Proteins 0.000 claims description 9
- 102000004856 Lectins Human genes 0.000 claims description 9
- 239000002523 lectin Substances 0.000 claims description 6
- 241001522232 Pinellia ternata Species 0.000 claims description 5
- 108091005804 Peptidases Proteins 0.000 claims description 4
- 239000004365 Protease Substances 0.000 claims description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 4
- 102000039446 nucleic acids Human genes 0.000 claims description 4
- 108020004707 nucleic acids Proteins 0.000 claims description 4
- 150000007523 nucleic acids Chemical class 0.000 claims description 4
- 239000003153 chemical reaction reagent Substances 0.000 claims description 3
- 101710186708 Agglutinin Proteins 0.000 claims description 2
- 101710146024 Horcolin Proteins 0.000 claims description 2
- 101710189395 Lectin Proteins 0.000 claims description 2
- 101710179758 Mannose-specific lectin Proteins 0.000 claims description 2
- 101710150763 Mannose-specific lectin 1 Proteins 0.000 claims description 2
- 101710150745 Mannose-specific lectin 2 Proteins 0.000 claims description 2
- 238000000034 method Methods 0.000 description 8
- 238000001262 western blot Methods 0.000 description 6
- 241000699666 Mus <mouse, genus> Species 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- 230000003053 immunization Effects 0.000 description 5
- 238000002649 immunization Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 238000001514 detection method Methods 0.000 description 4
- UQLDLKMNUJERMK-UHFFFAOYSA-L di(octadecanoyloxy)lead Chemical compound [Pb+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O UQLDLKMNUJERMK-UHFFFAOYSA-L 0.000 description 4
- 230000002163 immunogen Effects 0.000 description 4
- 235000018102 proteins Nutrition 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 238000008157 ELISA kit Methods 0.000 description 3
- 241000699670 Mus sp. Species 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 239000002953 phosphate buffered saline Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical class N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 230000007910 cell fusion Effects 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000209524 Araceae Species 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000009112 Mannose-Binding Lectin Human genes 0.000 description 1
- 108010087870 Mannose-Binding Lectin Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 108010089814 Plant Lectins Proteins 0.000 description 1
- 108010064851 Plant Proteins Proteins 0.000 description 1
- 206010035226 Plasma cell myeloma Diseases 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 239000000910 agglutinin Substances 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 230000000843 anti-fungal effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 201000000050 myeloid neoplasm Diseases 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000003726 plant lectin Substances 0.000 description 1
- 235000021118 plant-derived protein Nutrition 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000002331 protein detection Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 210000004989 spleen cell Anatomy 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/16—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from plants
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
- C07K2317/35—Valency
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
- C07K2317/565—Complementarity determining region [CDR]
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/415—Assays involving biological materials from specific organisms or of a specific nature from plants
- G01N2333/42—Lectins, e.g. concanavalin, phytohaemagglutinin
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Definitions
- the invention belongs to the field of biotechnology, and specifically relates to a Pinellia lectin protease-linked immune Elisa kit and its application.
- Pinellia ternate lectin is a monocotyledonous plant mannose-binding lectin protein among plant lectins.
- PTL is a plant protein with an amino acid sequence length of 256-258aa, a molecular weight of 25-27kDa, and an isoelectric point of 6-7. It has anti-insect, anti-fungal, anti-tumor and coagulation effects.
- PTL is extracted and purified from the fresh products of Pinellia ternata, an Araceae plant.
- the PTL detection kit of the present invention is an ELISA kit prepared with PTL monoclonal antibodies as raw materials. It can be used for the quantitative detection of PTL in samples. The method has high sensitivity, good repeatability, good specificity, simplicity, speed and low cost. Low, large batches of samples can be measured simultaneously.
- the antibody provided by the present invention for preparing an ELISA kit is a monoclonal antibody of PTL, and the antibody can be used to prepare a reagent for detecting pinellia lectin protein.
- the present invention provides a Pinellia lectin protease-linked immune Elisa kit and its application.
- the amino acid sequence of CDR-H1 of the heavy chain variable region of the above-mentioned 25E2E8 antibody is shown in SEQ ID NO:1
- the amino acid sequence of CDR-H2 is shown in SEQ ID NO:2
- the amino acid sequence of CDR-H3 is shown in SEQ ID NO As shown in: 3
- the amino acid sequence of CDR-L1 of the light chain variable region of the 25E2E8 antibody is shown in SEQ ID NO:5
- the amino acid sequence of CDR-L2 is shown in SEQ ID NO:6
- the amino acid sequence of CDR-L3 The amino acid sequence is shown in SEQ ID NO:7.
- amino acid sequence of the heavy chain of the above-mentioned 25E2E8 antibody is shown in SEQ ID NO:4, and the amino acid sequence of the light chain is shown in SEQ ID NO:8.
- the encoding gene of the 25E2E8 antibody heavy chain is shown in SEQ ID NO:9; the encoding gene of the light chain is shown in SEQ ID NO:10.
- the amino acid sequence of CDR-H1 of the heavy chain variable region of the above-mentioned 28E3D3 antibody is shown in SEQ ID NO:11
- the amino acid sequence of CDR-H2 is shown in SEQ ID NO:12
- the amino acid sequence of CDR-H3 is shown in SEQ ID NO :13
- the amino acid sequence of CDR-L1 of the light chain variable region of the 28E3D3 antibody is shown in SEQ ID NO:15
- the amino acid sequence of CDR-L2 is shown in SEQ ID NO:16
- the amino acid sequence of CDR-L3 The amino acid sequence is shown in SEQ ID NO:17.
- the amino acid sequence of the above-mentioned 28E3D3 antibody heavy chain is shown in SEQ ID NO:14
- the amino acid sequence of the 28E3D3 antibody light chain is shown in SEQ ID NO:18.
- the encoding gene of the 28E3D3 antibody heavy chain is shown in SEQ ID NO: 19; the encoding gene of the 28E3D3 antibody light chain is shown in SEQ ID NO: 20.
- the present invention provides a kit for detecting the protein content of Pinellia lectin. It is an enzyme-linked immunosorbent assay kit prepared from PTL monoclonal antibodies, which can achieve rapid and sensitive quantitative detection. PTL content in the sample.
- FIG. 1 SDS-PAGE diagram of purified monoclonal antibodies; M.Marker; 1.28E3D3 antibody; 2. Mouse IgG; 3.25E2E8 antibody; 4. Mouse IgG;
- Figure 2 shows the Western Blot diagram of 25E2E8 antibody; M.Marker; P.PTL; S. Pinellia ternata;
- Figure 3 shows the Western Blot diagram of 28E3D3 antibody; M.Marker; P.PTL; S. Pinellia ternata.
- the crude protein was purified by hydrophobic chromatography column and ion exchange column chromatography, dialyzed with a dialysis bag with a molecular weight of 7000 Da and then freeze-dried to obtain purified Pinellia lectin protein.
- the monoclonal antibody of the present invention is an IgG1 subtype murine monoclonal antibody, and the light chain type is ⁇ .
- the purity of monoclonal antibodies was analyzed by SDS-PAGE.
- Antibody titers were determined using indirect ELISA. Use the monoclonal antibodies 25E2E8 and 28E3D3 of the present invention as capture antibodies, coat the enzyme plate, leave it overnight at 4°C to adsorb the polystyrene plate holes, wash once with PBST and then block for 2 hours; PTL is diluted from 1000ng/mL. , add the coated wells, 100 ⁇ L/well, incubate at 37°C for 1 hour, and wash the plate with PBST 4 times; add biotin-labeled monoclonal antibodies 25E2E8 and 28E3D3 of the present invention, respectively, at 100 ⁇ L/well, incubate at 37°C for 1 hour, and wash the plate with PBST.
- the monoclonal antibodies 25E2E8 and 28E3D3 of the present invention were used as primary antibodies and their specific reactions were detected by Western blot method.
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- General Physics & Mathematics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Organic Chemistry (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Botany (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
L'invention concerne une trousse de dosage d'immunoabsorption par enzyme liée (test ELISA) de lectine de Pinellia ternata et une application, se rapportant au domaine de la biotechnologie. La trousse contient un anticorps monoclonal 25E2E8 et/ou un anticorps monoclonal 28E3D3 ; la séquence d'acides aminés de la chaîne lourde de l'anticorps 25E2E8 est telle que présentée dans la SEQ ID NO : 4, et la séquence d'acides aminés de la chaîne légère de cet anticorps est telle que présentée dans la SEQ ID NO : 8 ; la séquence d'acides aminés de la chaîne lourde de l'anticorps 28E3D3 est telle que présentée dans SEQ ID NO : 14, et la séquence d'acides aminés de la chaîne légère de l'anticorps 28E3D3 est telle que présentée dans SEQ ID NO : 18. La trousse est préparée à partir d'anticorps monoclonaux de lectine de Pinellia ternata et peut détecter rapidement, de manière sensible et quantitative, la teneur en lectine de Pinellia ternata d'un échantillon.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210362218.6A CN114720689B (zh) | 2022-04-07 | 2022-04-07 | 半夏凝集素蛋白酶联免疫Elisa试剂盒及应用 |
| CN202210362218.6 | 2022-04-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2023193376A1 true WO2023193376A1 (fr) | 2023-10-12 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/CN2022/109669 WO2023193376A1 (fr) | 2022-04-07 | 2022-08-02 | Trousse de dosage d'immunoabsoption par enzyme lié (test elisa) de lectine de pinellia ternata et application |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN114720689B (fr) |
| WO (1) | WO2023193376A1 (fr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114720689B (zh) * | 2022-04-07 | 2024-04-09 | 南京中医药大学 | 半夏凝集素蛋白酶联免疫Elisa试剂盒及应用 |
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| CN1300851A (zh) * | 2000-12-22 | 2001-06-27 | 复旦大学 | 一种半夏凝集素蛋白及其编码序列 |
| CN102343090A (zh) * | 2010-08-03 | 2012-02-08 | 张明熙 | 一种治疗类风湿性关节炎的医药组成物 |
| CN110684105A (zh) * | 2019-09-11 | 2020-01-14 | 江苏莱森生物科技研究院有限公司 | 一种抗hsp90单克隆抗体及试剂盒 |
| CN110845610A (zh) * | 2019-11-26 | 2020-02-28 | 武汉生物制品研究所有限责任公司 | 针对白喉类毒素的检测抗体对及其应用 |
| CN110927387A (zh) * | 2019-11-08 | 2020-03-27 | 江苏大学 | 一种brca2蛋白酶联免疫双抗夹心试剂盒及其应用 |
| CN111393526A (zh) * | 2020-03-30 | 2020-07-10 | 中国人民解放军第四军医大学 | 抗gdf15中和性单克隆抗体及其应用 |
| CN114720689A (zh) * | 2022-04-07 | 2022-07-08 | 南京中医药大学 | 半夏凝集素蛋白酶联免疫Elisa试剂盒及应用 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2295317A1 (fr) * | 1997-06-17 | 1998-12-23 | Human Genome Sciences, Inc. | Facteur de type hereguline |
| WO2007075914A2 (fr) * | 2005-12-22 | 2007-07-05 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Anticorps monoclonaux utilises contre des orthopoxvirus |
| JP2009178156A (ja) * | 2008-01-29 | 2009-08-13 | Miyazakiken Sangyo Shien Zaidan | クラスタリングによる蛋白質の有効性推定方法 |
| CN102277328A (zh) * | 2011-08-09 | 2011-12-14 | 华中农业大学 | 抗水稻白背飞虱的枯草芽胞杆菌工程菌及制备方法和应用 |
| DK3052522T3 (da) * | 2013-10-03 | 2020-02-24 | Biocare Medical Llc | Anti-sox 10 antistofsystemer og -fremgangsmåder |
| US20190382785A1 (en) * | 2018-05-09 | 2019-12-19 | FB Genetics (Pvt) Limited | Development of Herbicide and Sucking Pest Resistant Plant [Kalgin-5] by the Over-Expression of Constitutive Promoters Driven Tetra Gene Construct |
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2022
- 2022-04-07 CN CN202210362218.6A patent/CN114720689B/zh active Active
- 2022-08-02 WO PCT/CN2022/109669 patent/WO2023193376A1/fr unknown
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1300851A (zh) * | 2000-12-22 | 2001-06-27 | 复旦大学 | 一种半夏凝集素蛋白及其编码序列 |
| CN102343090A (zh) * | 2010-08-03 | 2012-02-08 | 张明熙 | 一种治疗类风湿性关节炎的医药组成物 |
| CN110684105A (zh) * | 2019-09-11 | 2020-01-14 | 江苏莱森生物科技研究院有限公司 | 一种抗hsp90单克隆抗体及试剂盒 |
| CN110927387A (zh) * | 2019-11-08 | 2020-03-27 | 江苏大学 | 一种brca2蛋白酶联免疫双抗夹心试剂盒及其应用 |
| CN110845610A (zh) * | 2019-11-26 | 2020-02-28 | 武汉生物制品研究所有限责任公司 | 针对白喉类毒素的检测抗体对及其应用 |
| CN111393526A (zh) * | 2020-03-30 | 2020-07-10 | 中国人民解放军第四军医大学 | 抗gdf15中和性单克隆抗体及其应用 |
| CN114720689A (zh) * | 2022-04-07 | 2022-07-08 | 南京中医药大学 | 半夏凝集素蛋白酶联免疫Elisa试剂盒及应用 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114720689A (zh) | 2022-07-08 |
| CN114720689B (zh) | 2024-04-09 |
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