WO2023175960A1 - 電気泳動装置 - Google Patents

電気泳動装置 Download PDF

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Publication number
WO2023175960A1
WO2023175960A1 PCT/JP2022/012810 JP2022012810W WO2023175960A1 WO 2023175960 A1 WO2023175960 A1 WO 2023175960A1 JP 2022012810 W JP2022012810 W JP 2022012810W WO 2023175960 A1 WO2023175960 A1 WO 2023175960A1
Authority
WO
WIPO (PCT)
Prior art keywords
contact
transport unit
jig
electrophoresis
electrophoresis device
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2022/012810
Other languages
English (en)
French (fr)
Japanese (ja)
Inventor
慎治 竹内
誉人 五味
基博 山崎
哲男 田村
顕行 根本
友成 盛岡
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hitachi High Tech Corp
Original Assignee
Hitachi High Tech Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi High Tech Corp filed Critical Hitachi High Tech Corp
Priority to DE112022006281.9T priority Critical patent/DE112022006281T5/de
Priority to JP2024507461A priority patent/JP7735535B2/ja
Priority to US18/842,441 priority patent/US20250180511A1/en
Priority to CN202280093183.9A priority patent/CN118843792A/zh
Priority to GB2412709.4A priority patent/GB2631180A/en
Priority to PCT/JP2022/012810 priority patent/WO2023175960A1/ja
Publication of WO2023175960A1 publication Critical patent/WO2023175960A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones

Definitions

  • the present invention relates to an electrophoresis apparatus, and relates to positional calibration when transporting a container containing a sample or a buffer solution.
  • An electrophoresis device is a device that separates a fluorescently labeled sample by electrophoresis within a capillary and analyzes the sample by detecting fluorescence induced by irradiation with excitation light.
  • Many electrophoresis apparatuses are equipped with an autosampler that transports containers containing samples and buffer solutions to predetermined positions. If the position of the container transported by the autosampler shifts, problems will occur in the injection of the sample into the capillary, so highly accurate position calibration is required.
  • Patent Document 1 discloses an autosampler that calibrates the position of the needle based on image data of the tip of the needle for inhaling and discharging the sample and the sample container taken from above, in which the needle moves from the initial position to the target. It is disclosed that a pulse signal until the object moves to a certain position is used as a calibration value.
  • Patent Document 1 requires an imaging device that captures image data for position calibration, and the system configuration of the electrophoresis device becomes complicated.
  • the present invention provides an electrophoresis apparatus that separates and analyzes samples by electrophoresis, which includes a transport section that transports a container containing the sample and a buffer solution to a target position; a contact portion with which a jig mounted on the transfer portion or a portion of the transfer portion comes into contact when the transfer portion moves to the target position; and a contact portion with which the jig or a portion of the transfer portion contacts the contact portion.
  • the present invention is characterized by comprising a control unit that records the distance from the position at the time of contact to the origin as a calibration value, and controls the movement of the conveyance unit based on the calibration value.
  • FIG. 1 is a perspective view showing an example of the overall configuration of an electrophoresis apparatus according to Example 1.
  • FIG. FIG. 3 is a perspective view showing an example of a movable jig and a fixed jig according to the first embodiment.
  • FIG. 3 is a diagram illustrating an example of the flow of processing in the first embodiment.
  • FIG. 3 is a perspective view showing contact between a movable jig and a fixed jig.
  • FIG. 7 is a diagram illustrating an example of the flow of processing in Example 2; The figure which shows an example of the excitation waveform of a motor.
  • FIG. 7 is a diagram illustrating an example of a fixed side jig of Example 3.
  • FIG. 7 is a diagram showing another example of the fixed side jig of Example 3.
  • the perspective view which shows an example of the pin and through-hole of a comparative example.
  • An electrophoresis device is a device that separates a fluorescently labeled sample by electrophoresis and analyzes the sample by detecting fluorescence induced by irradiation with excitation light.
  • the electrophoresis apparatus includes a transport unit 101 that transports a storage container 102 containing a sample and a buffer solution to a target position 103 that is accessed by a capillary tip 104, and a control unit 105 that is a computer that controls each part. .
  • a sample and a buffer solution are injected into the capillary from the capillary tip 104 and used for analysis.
  • highly accurate position calibration is performed by recording the distance from the position when the jig mounted on the transport unit 101 contacts the contact portion provided at the target position 103 to the origin as a calibration value. Ru. Note that the recorded calibration values are used for control to move the conveyance unit 101 to the target position 103.
  • the transport unit 101 slides on rails extending in the directions of the X-axis and the Y-axis by the driving force of the motor, and moves between the origin 200 and the target position 103.
  • the motor generates a holding force that keeps the conveying section 101 in its position when it is not energized, that is, when it is in a non-excited state.
  • the movable jig 201 is a jig mounted on the transport section 101, and has a protrusion 201A that protrudes upward.
  • the protrusion 201A has a prismatic shape with surfaces perpendicular to each of the X-axis, Y-axis, and Z-axis.
  • the fixed side jig 202 is a jig placed at the target position 103, and has a shape in which a part of a rectangular parallelepiped is cut into a rectangular parallelepiped shape, and the surface formed by cutting is a contact portion 202A.
  • the contact portion 202A is a surface with which the protrusion 201A of the movable jig 201 comes into contact.
  • the fixed side jig 202 may have a peephole 202B.
  • the peephole 202B is used for visually confirming the position of the movable jig 201.
  • the transport unit 101 is moved toward the target position 103.
  • the transport unit 101 is moved manually, for example, by an operator. Note that although the holding force is generated by the motor, it is possible to move the conveyance unit 101 manually.
  • FIG. 4 illustrates a state in which the protrusion 201A of the movable jig 201 located below the fixed jig 202 is in contact with the contact portion 202A of the fixed jig 202. Since it is not possible to visually confirm that the two are in contact, the presence or absence of contact is determined, for example, by the operator's sense of touch.
  • the motor is turned on. Note that immediately after the motor is turned on, the conveyance unit 101 may be slightly moved so that the movable jig 201 is separated from the fixed jig 202. Such slight movement can prevent damage to the protrusion 201A and the contact portion 202A.
  • the control unit 105 controls the transport unit 101 to return to the origin 200. Whether the transport unit 101 has returned to the origin 200 is detected by an optical sensor or the like installed at the origin 200.
  • the control unit 105 records the distance from the contact position, which is the position where the protrusion 201A contacts the contact portion 202A, to the origin 200 as a calibration value. Note that the distance from the contact position to the origin 200 is calculated based on the number of drive pulses to the motor counted while the conveyance section 101 returns from the contact position to the origin 200.
  • the distance from the position where the protrusion 201A contacts the contact portion 202A to the origin 200 is recorded as a calibration value, so highly accurate position calibration is possible.
  • the control unit 105 moves the transport unit 101 from the origin 200 to the target position 103 using the recorded calibration values. Furthermore, since calibration values can be recorded without using an imaging device, it is possible to simplify the system configuration of the electrophoresis device.
  • the calibration values may be recorded over time.
  • the control unit 105 can calculate and present the inspection timing of the electrophoresis apparatus. That is, based on changes in the calibration value over time, the control unit 105 can predict the time when the calibration value will exceed a predetermined threshold value as the inspection time.
  • Example 1 it has been described that the transport section 101 carrying the movable jig 201 is manually moved toward the target position 103, and the contact between the protrusion 201A and the contact section 202A is determined by tactile sensation.
  • the conveyance unit 101 is moved by driving a motor and the presence or absence of contact is determined using step-out of the motor. Note that some of the configurations and functions described in the first embodiment can be applied to the second embodiment, so similar configurations and functions will be denoted by the same reference numerals and descriptions thereof will be omitted.
  • the control unit 105 turns on the motor that drives the transport unit 101. Note that a movable jig 201 is mounted on the transport unit 101, and a fixed jig 202 is disposed at the target position 103.
  • the control unit 105 sends an instruction signal to the motor so that the transport unit 101 carrying the movable jig 201 moves toward the target position 103.
  • the control unit 105 determines whether the movement signal to the target position 103 has ended. If the process has been completed, the process advances to S305; if not, the process returns to S502. That is, the control unit 105 continues to move the conveyance unit 101 toward the target position 103 until the motor loses synchronization.
  • the input signal to the motor and the rotation of the motor will be out of synchronization, resulting in step-out. That is, it can be determined that the protrusion 201A has contacted the contact portion 202A when the motor is out of step.
  • the excitation method may be any of two-phase excitation, 1-2 phase excitation, and 1-phase excitation. However, in order to reduce damage caused by contact between the protrusion 201A and the contact portion 202A, one-phase excitation with relatively low movable torque is desirable.
  • FIG. 6 illustrates waveforms when rotating the stepping motor with one-phase excitation.
  • the first stage of the five waveforms is a clock pulse 600
  • the second to fifth stages are one-phase excitation waveforms 601 that excite each of the four stator coils of the stepping motor.
  • the stator coils in the second and fourth stages are arranged to face each other
  • the stator coils in the third and fifth stages are arranged to face each other.
  • by exciting with a modulated waveform 602 in which the peak value is lowered and the pulse width is narrowed the holding force of the stepping motor is reduced and step-out is more likely to occur.
  • the control unit 105 controls the transport unit 101 to return to the origin 200 as in the first embodiment. Note that the transport unit 101 may be slightly moved so that the movable jig 201 moves away from the fixed jig 202.
  • the control unit 105 records the distance from the position where the motor lost synchronization to the origin 200 as a calibration value.
  • the distance from the position where the motor lost synchronization to the origin 200 can be recorded as a calibration value without bothering the operator.
  • the recorded calibration values are used for control to move the conveyance unit 101 from the origin 200 to the target position 103, as in the first embodiment.
  • calibration values can be recorded without using an imaging device, it is possible to simplify the system configuration of the electrophoresis device.
  • the transport section 101 is moved by driving the motor, and the presence or absence of contact between the protrusion section 201A and the contact section 202A is determined using the step-out of the motor.
  • a contact sensor for detecting the presence or absence of contact between the protrusion 201A and the contact portion 202A is provided on the fixed side jig 202. Note that some of the configurations and functions described in the first and second embodiments can be applied to the third embodiment, so similar configurations and functions will be designated by the same reference numerals and descriptions thereof will be omitted.
  • the fixed side jig 202 of Example 3 has a shape in which a part of a rectangular parallelepiped is cut into a rectangular parallelepiped shape, and has a contact portion on the surface formed by cutting, and a contact sensor is installed in the contact portion. be done.
  • the contact sensors are installed for each plane perpendicular to each of the X-axis, Y-axis, and Z-axis.
  • a detection signal output from each contact sensor via a cable is amplified by an amplifier, converted to a digital signal by an AD converter, and transmitted to the control unit 105.
  • a contact sensor, an amplifier, an AD converter, and an SBC Single Board Computer
  • the detection signal output from the contact sensor is amplified and AD converted, and then transmitted to the control unit 105 via a wireless connection.
  • the process flow of the third embodiment is the same as that of the second embodiment.
  • the presence or absence of contact is determined based on the detection signal from the contact sensor, rather than using motor step-out.
  • the distance from the position where the protrusion 201A contacts the contact portion 202A to the origin 200 can be recorded as a calibration value without bothering the operator.
  • the recorded calibration values are used to control the movement of the transport unit 101 from the origin 200 to the target position 103. Furthermore, since calibration values can be recorded without using an imaging device, it is possible to simplify the system configuration of the electrophoresis device.
  • Example 1 the calibration value was recorded using the position where the protrusion 201A of the movable side jig 201 and the contact portion 202A of the fixed side jig 202 came into contact as a reference, and in Example 2, the calibration value was recorded using the position where the motor lost synchronization as a reference.
  • a comparative example of Examples 1 to 3 a case will be described in which a calibration value is recorded with reference to a position where a pin provided on the transport section 101 is penetrated through a hole provided at the target position 103. Note that some of the configurations and functions described in Examples 1 to 3 can be applied to the comparative example, so similar configurations and functions will be designated by the same reference numerals and descriptions thereof will be omitted.
  • FIG. 8 illustrates a pin 801 provided in the transport section 101 and a through hole 802 provided in the target position 103.
  • the pin 801 has a cylindrical shape and protrudes upward.
  • the through hole 802 is a circular through hole having an inner diameter smaller than the outer diameter of the pin 801.
  • the operator moves the transport unit 101 to the target position 103 and visually confirms that the pin 801 and the through hole 802 are in the penetrating state. Thereafter, the conveying unit 101 is returned to the origin 200, and the distance from the position where the penetrating state is reached to the origin 200 is recorded as a calibration value.
  • the recorded calibration values are used for control to move the conveyance unit 101 to the target position 103.
  • the reference position can be easily detected and the adjustment process becomes easier.
  • 101 Transport section
  • 102 Storage container
  • 103 Target position
  • 104 Capillary tip
  • 200 Origin
  • 201 Movable side jig
  • 201A Projection
  • 202 Fixed side jig
  • 202A Contact section
  • 202B Peephole
  • 600 Clock pulse
  • 601 1-phase excitation waveform
  • 602 Modulation waveform
  • 801 Pin
  • 802 Through hole

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
PCT/JP2022/012810 2022-03-18 2022-03-18 電気泳動装置 Ceased WO2023175960A1 (ja)

Priority Applications (6)

Application Number Priority Date Filing Date Title
DE112022006281.9T DE112022006281T5 (de) 2022-03-18 2022-03-18 Elektrophoresevorrichtung
JP2024507461A JP7735535B2 (ja) 2022-03-18 2022-03-18 電気泳動装置
US18/842,441 US20250180511A1 (en) 2022-03-18 2022-03-18 Electrophoresis Device
CN202280093183.9A CN118843792A (zh) 2022-03-18 2022-03-18 电泳装置
GB2412709.4A GB2631180A (en) 2022-03-18 2022-03-18 Electrophoresis device
PCT/JP2022/012810 WO2023175960A1 (ja) 2022-03-18 2022-03-18 電気泳動装置

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2022/012810 WO2023175960A1 (ja) 2022-03-18 2022-03-18 電気泳動装置

Publications (1)

Publication Number Publication Date
WO2023175960A1 true WO2023175960A1 (ja) 2023-09-21

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PCT/JP2022/012810 Ceased WO2023175960A1 (ja) 2022-03-18 2022-03-18 電気泳動装置

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US (1) US20250180511A1 (https=)
JP (1) JP7735535B2 (https=)
CN (1) CN118843792A (https=)
DE (1) DE112022006281T5 (https=)
GB (1) GB2631180A (https=)
WO (1) WO2023175960A1 (https=)

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07334245A (ja) * 1994-06-13 1995-12-22 Yotaro Hatamura 超精密送り装置およびこれを用いたｘｙテ−ブル並びにテ−ブル移送装置
JP2005531769A (ja) * 2002-06-28 2005-10-20 バイオヴェリス コーポレイション 改良された分析システム及びその構成要素
JP2006084411A (ja) * 2004-09-17 2006-03-30 Hitachi High-Technologies Corp 電気泳動装置、サンプルトレイ、及び、電気泳動方法
JP2008281365A (ja) * 2007-05-08 2008-11-20 Shimadzu Corp マイクロチップ電気泳動装置
WO2012157642A1 (ja) * 2011-05-16 2012-11-22 株式会社日立ハイテクノロジーズ 自動分析装置及び方法
JP2013117541A (ja) * 2013-02-26 2013-06-13 Sharp Corp 電気泳動用器具および電気泳動装置
JP2014119291A (ja) * 2012-12-14 2014-06-30 Hitachi High-Technologies Corp 自動分析装置
JP2021124443A (ja) * 2020-02-07 2021-08-30 株式会社日立ハイテク 自動分析装置

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014162921A1 (ja) 2013-04-01 2014-10-09 株式会社島津製作所 オートサンプラ

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07334245A (ja) * 1994-06-13 1995-12-22 Yotaro Hatamura 超精密送り装置およびこれを用いたｘｙテ−ブル並びにテ−ブル移送装置
JP2005531769A (ja) * 2002-06-28 2005-10-20 バイオヴェリス コーポレイション 改良された分析システム及びその構成要素
JP2006084411A (ja) * 2004-09-17 2006-03-30 Hitachi High-Technologies Corp 電気泳動装置、サンプルトレイ、及び、電気泳動方法
JP2008281365A (ja) * 2007-05-08 2008-11-20 Shimadzu Corp マイクロチップ電気泳動装置
WO2012157642A1 (ja) * 2011-05-16 2012-11-22 株式会社日立ハイテクノロジーズ 自動分析装置及び方法
JP2014119291A (ja) * 2012-12-14 2014-06-30 Hitachi High-Technologies Corp 自動分析装置
JP2013117541A (ja) * 2013-02-26 2013-06-13 Sharp Corp 電気泳動用器具および電気泳動装置
JP2021124443A (ja) * 2020-02-07 2021-08-30 株式会社日立ハイテク 自動分析装置

Also Published As

Publication number Publication date
DE112022006281T5 (de) 2025-02-27
JP7735535B2 (ja) 2025-09-08
CN118843792A (zh) 2024-10-25
GB2631180A (en) 2024-12-25
US20250180511A1 (en) 2025-06-05
GB202412709D0 (en) 2024-10-16
JPWO2023175960A1 (https=) 2023-09-21

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