WO2023115677A1 - 一种含碳工业气体工业化蛋白质生产系统 - Google Patents

一种含碳工业气体工业化蛋白质生产系统 Download PDF

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WO2023115677A1
WO2023115677A1 PCT/CN2022/073169 CN2022073169W WO2023115677A1 WO 2023115677 A1 WO2023115677 A1 WO 2023115677A1 CN 2022073169 W CN2022073169 W CN 2022073169W WO 2023115677 A1 WO2023115677 A1 WO 2023115677A1
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raw material
gas
thalline
material gas
mash
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PCT/CN2022/073169
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English (en)
French (fr)
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夏楠
董燕
陈琪
晁伟
宋庆坤
莫志朋
佟淑环
邹方起
李重阳
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北京首钢朗泽科技股份有限公司
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Priority to JP2022549205A priority Critical patent/JP2024502915A/ja
Priority to EP22751241.5A priority patent/EP4223380A1/en
Publication of WO2023115677A1 publication Critical patent/WO2023115677A1/zh

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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/26—Conditioning fluids entering or exiting the reaction vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/03—Means for pre-treatment of biological substances by control of the humidity or content of liquids; Drying
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/04—Phase separators; Separation of non fermentable material; Fractionation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/06—Means for pre-treatment of biological substances by chemical means or hydrolysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/20—Heating; Cooling
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/14—Drying

Definitions

  • the disclosure relates to a carbon-containing industrial gas industrialized protein production system, which belongs to the field of protein preparation.
  • the process of preparing fuel protein by treating gases containing CO/CO 2 /H 2 such as iron and steel industry tail gas, synthesis gas, and refining tail gas by microbial fermentation has been well received due to its reduced energy consumption, safety and efficiency. develop.
  • gases containing CO/CO 2 /H 2 such as iron and steel industry tail gas, synthesis gas, and refining tail gas by microbial fermentation
  • the final product in addition to by-product alcohols, the final product also has a large number of end-of-life cycle bacteria produced during microbial fermentation and after fermentation.
  • These end-of-life cycle bacteria form high-concentration protein wastewater.
  • the protein The concentration of wastewater can be as high as 10g/L-20g/L. If it is separated and recycled, it can also collect a large amount of protein, which has considerable economic benefits. If it is directly discharged, it will not only lead to the loss of protein substances, but also high-concentration protein water. It will also have some impact on the environment. However, the growth and metabolism of bacteria in the process of
  • the present disclosure provides a carbon-containing industrial gas industrialized protein production system, and by utilizing one or more embodiments of the present disclosure, the technical problem of low protein yield in the existing microbial fermentation process for protein preparation is solved.
  • the present disclosure provides a carbon-containing industrial gas industrialized protein production system, including: a raw material gas purification system, used to remove impurities in the raw material gas; a water purification system, used to kill miscellaneous bacteria in the raw material water; a bacteria preparation system, It is connected with the raw material gas purification system and water purification system and is used to ferment the raw material gas and raw material water to obtain mash; the bacteria separation system is connected with the said bacteria preparation system and used to separate the mash Bacteria and alcohol; protein preparation system, communicated with the thalline separation system and used for making thallines into thalline protein products.
  • the disclosure guarantees the removal of impurities in the raw material gas by purifying the raw material gas and raw material water, provides high-quality raw materials for the growth and reproduction of bacteria, removes miscellaneous bacteria in the raw material gas, and provides an excellent environment for the growth and reproduction of bacteria, so that the raw material gas High-efficiency fermentation is carried out in the cell preparation system, and high-quality cell protein is obtained through the cell separation system and protein preparation system, thereby increasing the protein yield.
  • FIG. 1 is a schematic diagram of a carbon-containing industrial gas industrial protein production system according to some embodiments of the present disclosure
  • FIG. 2 is a schematic diagram of a carbon-containing industrial gas industrial protein production system according to other embodiments of the present disclosure.
  • 1-raw material gas purification system 111-first dedusting device, 112-second dedusting device, 121-first adsorption device, 122-electric heater, 123-second adsorption device, 124-vacuum pump, 13-raw material gas control device, 14-water separator;
  • 2-water purification system 21-gas sterilization device, 22-chemical sterilization device, 23-purified water delivery pump;
  • 3-cell preparation system 31-cell preparation device, 32-cell transport pump, 33-cell concentration device;
  • the present disclosure provides a carbon-containing industrial gas industrialized protein production system, as shown in Figure 1, which may include:
  • the raw material gas purification system 1 can be used to remove impurities in the raw material gas
  • Water purification system 2 which can be used to kill miscellaneous bacteria in raw water
  • the bacterial cell preparation system 3 can communicate with the raw material gas purification system 1 and the water purification system 2 and can be used to ferment the raw material gas and raw water as raw materials to obtain mash;
  • a thallus separation system 4 communicated with the thallus preparation system 3 and capable of separating the thallus and alcohol from the mash;
  • the protein production system 5 communicates with the thalline separation system 4 and can be used to make thalline protein products from the thallus.
  • the raw material gas purification system 1 can purify the raw material gas, and the water purification system 2 can also purify the raw material water.
  • the purified raw material gas and raw water enter the bacterial preparation system 3, and the bacterial cells use raw material gas and raw water as raw materials Fermentation is carried out to obtain mash, which contains bacteria, fermentation products such as alcohols, impurities and water, and then the mash enters the bacteria separation system 4, where bacteria and alcohol are separated from the bacteria, and impurities and water are discharged , the alcohol enters into other process systems, and the bacteria enter the protein preparation system 5 as protein to make protein products from the bacteria, which can be used as feed to feed animals.
  • the raw material gas may include carbon-containing industrial gas and hydrogen.
  • the raw material gas includes CO, CO 2 and H 2
  • the raw material gas purification system 1 removes impurities therein, and the impurities include tar, benzene series, oxygen and halides to prevent them from affecting the growth of bacteria, and at the same time remove the dust in the raw material gas purification system 1 to prevent dust from entering the bacteria preparation system 3 and affecting the purity of the protein products of the bacteria.
  • miscellaneous bacteria in the raw water such as methane bacteria, will compete with the bacteria for carbon sources, occupy the living space of the bacteria, and affect the normal growth and reproduction of the bacteria.
  • Removing miscellaneous bacteria in the raw water through the water purification system 2 can ensure the purity of the bacteria in the bacteria preparation system 3, improve the yield and purity of protein, and at the same time enable the carbon-containing industrial gas industrialized protein production system to run efficiently for a long time .
  • the raw gas purification system 1 may include:
  • Raw gas dedusting device which can be used to remove dust and tar in raw gas
  • a raw material gas impurity purification device which communicates with the raw material gas dedusting device and can be used to remove benzene series, halides and oxygen in the raw material gas;
  • a raw material temperature control device 13 the raw material gas impurity purification device is connected and can be used to control the temperature of the raw material gas.
  • the raw material temperature control device 13 may be a steam heat exchanger;
  • the water separator 14 is in communication with the raw material air temperature control device 13 and the bacterial cell preparation system 3 and can be used to remove moisture in the raw material gas.
  • the raw material gas dust removal device may include a first dust removal device 111 and a second dust removal device 112, the first dust removal device 111 may be a bag filter, and the raw material gas impurity purification device may include a first adsorption device 121 , an electric heater 122 and a second adsorption device 123 .
  • the raw material gas can enter from the first dedusting device 111, remove most of the dust particles in the first dedusting device 111, obtain the purified gas with dust content ⁇ 5 mg/m 3 , then enter the second dedusting device 112, and in the second dedusting device 112
  • the device 112 further removes dust and removes part of the tar to obtain a purified gas with a dust content of ⁇ 1 mg/m 3 and a tar content of ⁇ 1 mg/m 3 , which can then enter the first adsorption device 121 to remove benzene series impurities in the raw gas.
  • Purified gas with benzene series content ⁇ 0.1ppm can be obtained, then it can be heated by electric heater 122 and enter the second adsorption device 123, and halides can be removed in the second adsorption device 123 to obtain purification of halides ⁇ 0.1ppm and oxygen ⁇ 1000ppm Then, it can enter the raw material air temperature control device 13 to cool down, so that its temperature can drop to 32°C-40°C, and finally it can enter the gas-liquid separation device to separate the liquid water in the raw material gas.
  • the raw material gas enters the cell preparation system 3 and can be used as Raw materials are fermented.
  • the dust and impurities in the raw material gas can be removed through the raw material gas dedusting device and the raw material gas impurity purification device, so as to prevent the dust from affecting the purity of the cell protein product, and prevent the impurity from affecting the growth and reproduction of the cell, thereby increasing the yield of the cell protein, and at the same time
  • the temperature of the raw material gas can be controlled at 32°C-40°C, which is suitable for the growth of bacteria, so as to prevent the excessively high or
  • the liquid separation device can perform gas-liquid separation.
  • the outlet of the electric heater 122 can also be connected to the first adsorption device 121, and when the first adsorption device 121 or the second adsorption device 123 needs to be regenerated, the regeneration gas is passed into the inlet of the electric heater 122, The temperature of the regeneration gas can be raised to the regeneration temperature to facilitate the regeneration.
  • Adsorption under pressure using decompression or normal pressure desorption method, is called pressure swing adsorption; pressure swing adsorption operation due to the small thermal conductivity of the adsorbent, the adsorption heat and desorption heat caused by the adsorbent bed
  • the temperature does not change much, so it can be regarded as an isothermal process. Its working condition is approximately along the normal temperature adsorption isotherm, with adsorption at higher pressure and desorption at lower pressure.
  • the vacuum pump 124 can also be used to pressurize it to improve the adsorption efficiency; the first adsorption device 121 or the second adsorption device 123 When regeneration is required, the gas in the first adsorption device 121 or the second adsorption device 123 can also be pumped out by the vacuum pump 124 to reduce the air pressure therein and improve its desorption efficiency.
  • the water purification system 2 may include:
  • a gas sterilizing device 21 which communicates with the raw material gas purification system 1 and can be used to pass sterilizing gas into the raw material water;
  • a chemical sterilizer 22 is in communication with the gas sterilizer 21 and the bacterium preparation system 3 and can be used to add a sterilant to the raw water;
  • the purified water delivery pump 23 is arranged between the chemical sterilization device 22 and the bacterial cell preparation system 3 and can be used to pump purified water from the chemical sterilization device 22 into the bacterial cell preparation system 3 .
  • the top of the gas sterilizer 21 communicates with the process water inlet, the bottom of the gas sterilizer 21 communicates with the gas inlet, and the upper part of the gas sterilizer 21 communicates with the chemical sterilizer 22 through overflow, so
  • the top of the chemical sterilization device 22 is provided with a fungicide inlet, and the outlet of the chemical sterilization device 22 is in communication with the inlet of the purified water delivery pump 23 .
  • the raw water first enters the gas sterilizing device 21, and by passing ozone into the gas sterilizing device 21, the ozone can oxidize the colonies in the raw water, and then pass into the chemical sterilizing device 22, and pass through the chemical sterilizing device 21. Adding a bactericide to the device 22 can more thoroughly kill the colonies in the raw water, and obtain purified water with the total number of colonies ⁇ 1 cfu/g.
  • the raw water is sampled at the outlet of the chemical sterilization device 22, and the number of colonies and the content of the fungicide in the raw water are detected to make the total number of colonies ⁇ 1cfu/g, and the content of the fungicide is calculated by mass fraction 0.002 ⁇ -0.01 ⁇ .
  • the embodiments of the present disclosure disclose that the raw water is passed into the gas sterilization device 21 and the chemical sterilization device 22 in sequence. In other embodiments, the raw water can also be passed into the chemical sterilization device 22 first, and then into the gas sterilization device The device 21 does not affect the implementation of the technical solution of the present disclosure.
  • the thalline preparation system 3 includes:
  • the bacterial cell preparation device 31 is in communication with the raw material gas purification system 1 and the water purification system 2 and can be used to ferment the raw material gas and raw material water under the action of the bacterial cells to obtain mash.
  • the feed port of the thalline preparation device 31 is respectively connected to the outlet of the water separator 14 and the outlet of the purified water delivery pump 23, and the thalline preparation device 31 can be a bioreactor;
  • a bacterial cell delivery pump 32 which is in communication with the bacterial cell preparation device 31 and the bacterial cell separation system 4 and can be used to pump the mash in the bacterial cell preparation device 31 into the bacterial cell separation system 4;
  • the cell thickening device 33 is in communication with the cell transport pump 32 and the cell preparation device 31 and can be used to separate the mash into thick mash and clear mash, and adjust the mash in the cell preparation device 31
  • Concentration and liquid level, in the embodiment of the present disclosure, the bacteria concentration device 33 may be a concentrator or a filter.
  • the purified gas enters the bacterial cell preparation device 31 from the outlet of the water separator 14, and the purified water enters the bacterial cell preparation device 31 from the outlet of the purified water delivery pump 23.
  • the raw material gas and raw water generate ethanol under the action of the bacterial cell, and the bacterial cell Propagate therein, thalline, ethanol, water and impurity are mixed as mash, send into thalline delivery pump 32, and thalline delivery pump 32 is divided into two strands when outputting, and one enters thalline separation system 4, and another strand enters bacterium body concentration device 33, the mash enters the cell concentration device 33 and is separated into thick mash and clear mash, and the thick mash is reused to the cell preparation device 31, which can be used to adjust the mash in the cell preparation device 31 concentration and liquid level, the clear mash is sent to other processes for treatment.
  • the thick mash is separated from the cell concentration device 33 and reused to the cell concentration device 33, which can improve the cell concentration device
  • the concentration of the mash in 33 when the concentration of the mash in the thalline preparation device 31 increases, the supply of raw water can also be increased to reduce the concentration of the mash.
  • the liquid level of the mash in the bacterial cell preparation device 31 can be controlled by controlling the operation of the bacterial cell transport pump 32, the supply of raw water, and the amount of concentrated mash reused by the bacterial cell concentration device 33; It is also possible to obtain high-concentration bacteria with a concentration of 3%-6%.
  • the thalline isolation system 4 may include:
  • Distillation and separation device 41 which communicates with the cell preparation system 3 and can be used to separate the alcohol in the mash, wherein the temperature at the bottom of the distillation and separation device 41 is ⁇ 90°C, and the pressure at the top is ⁇ -50kPa, which can be to distill ethanol;
  • the centrifugal separation device 42 communicates with the distillation separation device 41 and the protein production system 5 and is used to separate the bacteria in the mash.
  • the centrifugal separation device 42 may include a disc type One or more of centrifuge, decanter centrifuge, organic membrane or inorganic membrane;
  • the distillation bottom liquid delivery pump 43 communicates with the centrifugal separation device 42 through the distillation bottom liquid delivery pump 43 and can be used to send the distilled bottom liquid containing bacteria into the centrifugal separation device 42 .
  • the existing protein preparation method usually directly distills the mash prepared by the cell preparation system 3, and the water-soluble ions cannot be evaporated by water, and will still remain in the solid obtained after evaporation, so that the obtained cell protein A large amount of impurities remain in the product, which affects the purity of the product.
  • the alcohol in the mash is distilled out first, and the remaining impurities, bacteria and water go to the centrifugal separation device 42, and the centrifugal separation device 42 separates water and water-soluble impurities by centrifugation to obtain a concentration of 15%. -30% higher concentration of bacteria, the yield of bacteria > 95%, and then sent to the protein preparation system 5.
  • the protein preparation system 5 includes:
  • the drying device 51 is in communication with the cell separation system 4 and is used to dry the cells to prepare cell protein, wherein the drying temperature of the drying device 51 is ⁇ 200°C.
  • the drying device Can include one or more of spray drying, pressure drying, fluidized bed drying and drum scraper drying;
  • the packing device 52 communicates with the drying device 51 and can be used to pack the bacterial protein into a bacterial protein product.
  • the drying temperature of the drying device 51 is set to be less than or equal to 200°C.
  • a carbon-containing industrial gas industrialized protein production system of the present disclosure will be described in detail below in combination with examples, comparative examples and experimental data.
  • the cell protein is obtained by drying and packaging the finished product.
  • the raw material gas and water are not purified, no cell concentration device is installed, and the evaporation concentration process is adopted.
  • the raw material gas and water are directly passed into the cell preparation system 3 to obtain low-concentration cells and clear liquid with a concentration of 1%-2%;
  • the low-concentration cells are mixed and enter the distillation separation device to obtain low-concentration cells with a concentration of 1.2%-2.3%;
  • the concentrated bacterial liquid produced by the distillation and separation device is evaporated and concentrated to obtain high-concentration bacterial cells with a concentration of 6%-10%;
  • the cell protein After drying and packaging the finished product, the cell protein is obtained.
  • Table 1 shows the continuous and stable running time of the cell preparation system 3 of each embodiment and the comparative example, cell protein crude protein, ash content and yield, and steam consumption per ton of product.
  • the carbon-containing industrial gas industrialized protein production system purifies the raw material gas and raw water, removes impurities in the raw material gas, provides high-quality raw materials for the growth and reproduction of bacteria, removes miscellaneous bacteria in the raw material water, and provides high-quality raw materials for the growth and reproduction of bacteria.
  • the protein quality is high, the crude protein content is ⁇ 85%, and the ash content is ⁇ 1%. decrease; and the continuous and stable operation time of the bacterial cell preparation system 3 is >12 months, and the comprehensive efficiency is high.

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Abstract

一种含碳工业气体工业化蛋白质生产系统，属于蛋白质制取领域，包括菌体制备系统(3)、原料气净化系统(1)、水净化系统(2)、菌体分离系统(4)和蛋白质制取系统(5)，菌体制备系统(3)与原料气净化系统(1)、水净化系统(2)和菌体分离系统(4)分别连通，蛋白质制取系统(5)与菌体分离系统(4)连通。通过净化原料气和原料水，除去原料气中杂质和原料水中杂菌，为菌体繁殖提供优良原料和环境，使原料气高效发酵，从而提升蛋白质产率。

Description

一种含碳工业气体工业化蛋白质生产系统
相关申请的交叉引用
本公开要求于2021年12月20日提交、申请号为202111559296.7且名称为“一种含碳工业气体工业化蛋白质生产系统”的中国专利申请的优先权，其全部内容通过引用合并于此。
技术领域
本公开内容涉及一种含碳工业气体工业化蛋白质生产系统，属于蛋白质制取领域。
背景技术
目前，通过微生物发酵法处理钢铁工业尾气、合成气、炼化尾气等含CO/CO 2/H 2的气体来制备燃料蛋白质的工艺，由于其减少了能源消耗，安全高效，得到了较好的发展。该工艺过程中，最终产物除了副产物醇类外，还有在微生物发酵过程中以及发酵完成后产生的大量生命周期结束的细菌，这些生命周期结束的细菌形成了高浓度的蛋白废水，该蛋白废水浓度可高达10g/L-20g/L，若经过分离和回收处理也能够收集较大产量的蛋白质，具有可观的经济效益，而若是直接排放不仅会导致蛋白物质的流失，而且高浓度蛋白水也会对环境造成一定的影响。但现有的微生物发酵制备蛋白质过程中的菌体生长代谢不良，蛋白质产率不高。
发明内容
本公开内容提供了一种含碳工业气体工业化蛋白质生产系统，通过利用本公开内容的一个或多个实施方式解决了现有微生物发酵制备蛋白质过程中的蛋白质产率不高的技术问题。
本公开提供了一种含碳工业气体工业化蛋白质生产系统，包括：原料气净化系统，用以除去原料气中的杂质；水净化系统，用以杀灭原料水中的杂菌；菌体制备系统，与所述原料气净化系统以及水净化系统连通并用以原料气和原料水为原料进行发酵，得到醪液；菌体分离系统，与所述菌体制备 系统连通并用以将所述醪液分离出菌体和醇；蛋白质制取系统，与所述菌体分离系统连通并用以将菌体制成菌体蛋白质产品。
本公开通过净化原料气和原料水，保证除去原料气中的杂质，为菌体生长繁殖提供优质原料，除去原料气中的杂菌，为菌体生长繁殖提供优良环境，进而使原料气在菌体制备系统中进行高效的发酵，并通过菌体分离系统和蛋白质制取系统获得高质量的菌体蛋白质，从而提升蛋白质产率。
附图说明
此处的附图被并入说明书中并构成本说明书的一部分，示出了符合本发明的实施例，并与说明书一起用以解释本发明的原理。
为了更清楚地说明本发明实施例或现有技术中的技术方案，下面将对实施例或现有技术描述中所需要使用的附图作简单地介绍，显而易见地，对于本领域普通技术人员而言，在不付出创造性劳动性的前提下，还可以根据这些附图获得其他的附图。
图1是依据本公开内容的一些实施方式的一种含碳工业气体工业化蛋白质生产系统的示意图；
图2是依据本公开内容的另一些实施方式的一种含碳工业气体工业化蛋白质生产系统的示意图。
附图标记：
1-原料气净化系统，111-第一除尘装置，112-第二除尘装置，121-第一吸附装置，122-电加热器，123-第二吸附装置，124-真空泵，13-原料气温控装置，14-水分离器；
2-水净化系统，21-气体灭菌装置，22-化学灭菌装置，23-净化水输送泵；
3-菌体制备系统，31-菌体制备装置，32-菌体输送泵，33-菌体提浓装置；
4-菌体分离系统，41-蒸馏分离装置，42-离心分离装置，43-蒸馏底液输送泵；
5-蛋白质制取系统，51-干燥装置，52-包装装置。
具体实施方式
为使本申请实施例的目的、技术方案和优点更加清楚，下面将结合本申请实施例中的附图，对本申请实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例是本申请的一部分实施例，而不是全部的实施例。基于本申请中的实施例，本领域普通技术人员在没有做出创造性劳动的前提下所获得的所有其他实施例，都属于本申请保护的范围。
本公开内容提供了一种含碳工业气体工业化蛋白质生产系统，如图1所示，可以包括：
原料气净化系统1，能够用以除去原料气中的杂质；
水净化系统2，能够用以杀灭原料水中的杂菌；
菌体制备系统3，可以与所述原料气净化系统1以及水净化系统2连通并且能够用以将原料气和原料水作为原料进行发酵，得到醪液；
菌体分离系统4，与所述菌体制备系统3连通并且能够用以将所述醪液分离出菌体和醇；以及
蛋白质制取系统5，与所述菌体分离系统4连通并且能够用以将菌体制成菌体蛋白质产品。
原料气净化系统1可以对原料气进行净化，同时水净化系统2还可以对原料水进行净化，净化后的原料气和原料水进入菌体制备系统3，菌体以原料气和原料水为原料进行发酵，得到醪液，醪液中含有菌体、发酵的产物如醇类等、杂质和水，然后醪液进入菌体分离系统4，在菌体分离出菌体和醇，排出杂质和水，醇进入其他工艺系统，菌体作为蛋白质进入蛋白质制取系统5制成菌体蛋白质产品，可以作为饲料喂食动物。
在本公开实施方式中，所述原料气可以包括含碳工业气体和氢气，在一些实施方式中，原料气包括CO、CO 2和H 2，经过原料气净化系统1除去其中的杂质，杂质包括焦油、苯系物、氧气和卤化物，防止其影响菌体的生长，同时除去原料气净化系统1中的粉尘，防止粉尘进入菌体制备系统3，影响菌体蛋白质产品的纯度。同时，原料水中的杂菌如甲烷菌等会与菌体抢夺碳源，挤占菌体的生存空间，影响菌体的正常生长繁殖，一方面造成蛋白质产率下降，发酵产生的醇产率下降，另一方面，导致菌体衰退甚至全部死亡，使得 系统连续运行的时间不能得到保证。通过水净化系统2除去原料水中的杂菌，能够保证菌体制备系统3中菌体的纯净度，提高蛋白质的产率和纯度，同时还能够使含碳工业气体工业化蛋白质生产系统长时间高效运行。
在一些实施方式中，如图2所示，所述原料气净化系统1可以包括：
原料气除尘装置，能够用以除去原料气中的粉尘和焦油；
原料气杂质净化装置，与所述原料气除尘装置连通并且能够用以除去原料气中的苯系物、卤化物和氧气；
原料气温控装置13，所述原料气杂质净化装置连通并且能够用以对原料气的温度进行控制，在本公开实施方式中，原料气温控装置13可以是蒸汽换热器；
水分离器14，与所述原料气温控装置13以及菌体制备系统3连通并且能够用以除去原料气中的水分。
在本公开实施方式中，原料气除尘装置可以包括第一除尘装置111和第二除尘装置112，所述第一除尘装置111可以是布袋除尘器，原料气杂质净化装置可以包括第一吸附装置121、电加热器122和第二吸附装置123。原料气可以自第一除尘装置111进入，在第一除尘装置111中脱除大部分粉尘颗粒，获得含尘量＜5mg/m 3的净化气，然后进入第二除尘装置112，在第二除尘装置112中进一步除尘并且除去部分焦油，获得含尘量≤1mg/m 3、焦油含量≤1mg/m 3的净化气，接着可以进入第一吸附装置121，除去原料气中的苯系物杂质，获得苯系物含量≤0.1ppm的净化气，然后可以通过电加热器122加热进入第二吸附装置123，在第二吸附装置123中除去卤化物，获得卤化物≤0.1ppm，氧气≤1000ppm的净化气，然后可以进入原料气温控装置13中降温，使其温度下降至32℃-40℃，最后可以进入气液分离装置分离出原料气中的液态水，原料气进入菌体制备系统3可以作为原料进行发酵。
通过原料气除尘装置和原料气杂质净化装置能够除去原料气中的粉尘和杂质，防止粉尘影响菌体蛋白质产品的纯度，防止杂质影响菌体的生长繁殖，进而提升菌体蛋白质的产率，同时通过原料气温控装置13，能够将原料气的温度控制在适宜菌体生长的32℃-40℃，防止原料气温度过高或过低影响菌体的繁殖效率，同时使原料气温度下降使得气液分离装置可以进行气液分 离。
在本公开一些实施方式中，电加热器122的出口还可以连接第一吸附装置121，第一吸附装置121或第二吸附装置123需要再生时，将再生气通入电加热器122的入口，能够使再生气升温至再生温度，以方便再生。
在加压的情况下吸附，用减压或常压解吸的方法，称为变压吸附；变压吸附操作由于吸附剂的热导率较小，吸附热和解吸热所引起的吸附剂床层温度变化不大，故可将其看成等温过程，它的工况近似地沿着常温吸附等温线进行，在较高压力下吸附，在较低压力下解吸。在本公开一些实施方式中，在第一吸附装置121或第二吸附装置123需要吸附时，还可以通过真空泵124向其中加压，提升吸附效率；在第一吸附装置121或第二吸附装置123需要再生时，还可以通过真空泵124抽出第一吸附装置121或第二吸附装置123内的气体，降低其中的气压，提升其解吸效率。
在一些实施方式中，所述水净化系统2可以包括：
气体灭菌装置21，与所述原料气净化系统1连通并且能够用以向原料水中通入灭菌气体；
化学灭菌装置22，与所述气体灭菌装置21和所述菌体制备系统3连通并且能够用以向原料水中加入灭菌剂；
净化水输送泵23，设于化学灭菌装置22和菌体制备系统3之间并且能够用以将净化水从化学灭菌装置22泵入菌体制备系统3。
所述气体灭菌装置21顶部和工艺水进口连通，所述气体灭菌装置21底部和气体进口连通，所述气体灭菌装置21中上部通过溢流和所述化学灭菌装置22连通，所述化学灭菌装置22顶部设有杀菌剂进口，化学灭菌装置22出口和所述净化水输送泵23进口连通。
在本公开实施方式中，原料水先进入气体灭菌装置21，通过向气体灭菌装置21通入臭氧，能够使臭氧氧化原料水中的菌落，然后通入化学灭菌装置22，通过向化学灭菌装置22中加入杀菌剂，能够更彻底地杀灭原料水中的菌落，获得菌落总数≤1cfu/g的净化水。在另一些实施方式中，在化学灭菌装置22的出口对原料水进行取样，检测原料水中的菌落数和杀菌剂的含量，使菌落总数≤1cfu/g，杀菌剂的含量为以质量分数计的0.002‰-0.01‰。
本公开实施方式公开的是原料水依次通入气体灭菌装置21和化学灭菌装置22，在另一些实施方式中，还可以将原料水先通入化学灭菌装置22，再通入气体灭菌装置21，均不影响本公开技术方案的实施。
在一些实施方式中，所述菌体制备系统3包括：
菌体制备装置31，与所述原料气净化系统1以及水净化系统2连通并且能够用以在菌体的作用下以原料气和原料水为原料进行发酵，得到醪液，在本公开实施方式中，菌体制备装置31的进料口分别连通水分离器14的出口以及净化水输送泵23的出口，菌体制备装置31可以是生物反应器；
菌体输送泵32，与所述菌体制备装置31以及菌体分离系统4连通并且能够用以将所述菌体制备装置31内醪液泵入菌体分离系统4；
菌体提浓装置33，与所述菌体输送泵32以及菌体制备装置31连通并且能够用以将醪液分离为浓醪液和清醪液，并调节菌体制备装置31中的醪液浓度和液位，在本公开实施方式中，所述菌体提浓装置33可以是浓缩机，还可以是过滤器。
净化气自水分离器14的出口进入菌体制备装置31，净化水自净化水输送泵23的出口进入菌体制备装置31，原料气和原料水在菌体的作用下生成乙醇，同时菌体在其中繁殖，菌体、乙醇、水以及杂质混合作为醪液，送入菌体输送泵32，菌体输送泵32输出时分为两股，一股进入菌体分离系统4，另一股进入菌体提浓装置33，醪液进入菌体提浓装置33后分离成浓醪液和清醪液，浓醪液回用至菌体制备装置31，能够用以调节菌体制备装置31中醪液的浓度和液位，清醪液送去其他工艺处理。
当菌体繁殖速率降低时，由于菌体制备装置31中不断排出的醪液中包括菌体和生成物醇，因此仅靠菌体输送泵32无法调节菌体制备装置31中的醪液浓度，进而导致菌体的繁殖速率不断下降，如此恶性循环最终会导致反应停止，难以维持生产-产出的平衡点，使得含碳工业气体工业化蛋白质生产系统无法长时间运行。设置菌体提浓装置33，当菌体制备装置31中醪液浓度降低时，通过菌体提浓装置33分离出浓醪液回用至菌体提浓装置33，可以提升菌体提浓装置33中的醪液浓度；当菌体制备装置31中醪液的浓度提高时，还可以增加原料水的供应，以降低醪液的浓度。一方面通过控制菌体输送泵 32工作、原料水的供应量以及菌体提浓装置33回用至的浓醪液的量，可以控制菌体制备装置31中醪液的液位，另一方面还可以获得浓度3％-6％高浓菌体。
在一些实施方式中，所述菌体分离系统4可以包括：
蒸馏分离装置41，与所述菌体制备系统3连通并且能够用以分离所述醪液中的醇，其中，所述蒸馏分离装置41底部的温度≤90℃，顶部的压力≤-50kPa，可以使乙醇馏出；
离心分离装置42，与所述蒸馏分离装置41和所述蛋白质制取系统5连通并且用以分离醪液中的菌体，在本公开实施方式中，所述离心分离装置42可以包括碟片式离心机、卧螺式离心机、有机膜或无机膜中的一种或几种；
蒸馏底液输送泵43，通过蒸馏底液输送泵43与离心分离装置42连通并且能够用以将蒸馏后的包含菌体的底液送入离心分离装置42。
现有的蛋白质制备方法通常经过菌体制备系统3制备得到的醪液直接蒸馏，可溶于水的离子无法被水蒸发，依旧会残留在蒸发后得到的固体中，使得制备得到的菌体蛋白质产品中残留大量杂质，影响产品的纯度。本公开实施方式通过先蒸馏，将醪液中的醇馏出，剩余的杂质、菌体和水去到离心分离装置42，离心分离装置42通过离心将水以及水溶性杂质分离，获得浓度15％-30％的更高浓度菌体，菌体产率＞95％，然后送入蛋白质制取系统5。
在一些实施方式中，所述蛋白质制取系统5包括：
干燥装置51，与所述菌体分离系统4连通并且用以对菌体进行干燥，制备成菌体蛋白质，其中，所述干燥装置51干燥温度≤200℃，在本公开实施方式中，干燥装置可以包括喷雾干燥、压力干燥、流化床干燥和滚筒刮板干燥中的一种或多种；
包装装置52，与所述干燥装置51连通并且能够用以将菌体蛋白质包装成菌体蛋白质产品。
在本公开实施方式中，为了防止蛋白质高温分解，干燥装置51的干燥温度设置为小于或等于200℃。
下面将结合实施例、对比例及实验数据对本公开的一种含碳工业气体工业化蛋白质生产系统进行详细说明。
实施例1
S1、获得含尘量≤1mg/m 3、卤化物≤0.1ppm、温度32℃-40℃、不含液态水的净化气；
S2、获得菌落总数≤1cfu/g的净化水；
S3、获得浓度3％-6％高浓菌体；
S4、获得浓度15％-30％的更高浓度菌体，菌体产率＞95％；
S5、经干燥和成品包装获得菌体蛋白质。
对比例1
在本对比例中，原料气和水不进行净化，未设置菌体提浓装置、采用蒸发浓缩工艺。
S1、原料气和水直接通入菌体制备系统3，获得浓度1％-2％的低浓菌体和清液；
S2、低浓度菌体混合后进入蒸馏分离装置，获得浓度1.2％-2.3％的低浓菌体；
S3、蒸馏分离装置所产出的菌体浓液经蒸发浓缩，获得浓度6％-10％的高浓度菌体；
S4、经干燥和成品包装，获得菌体蛋白质。
计算各实施例与对比例菌体制备系统3连续稳定运行时间、菌体蛋白质粗蛋白、灰分和产率、每吨产品蒸汽消耗量情况如表1所示。
表1
Figure PCTCN2022073169-appb-000001
由表1的数据可知：对比例1中，原料气和水直接通入菌体制备系统3、未设置菌体提浓装置、蒸馏分离装置所产出的菌体浓液经蒸发浓缩，菌体制备系统3连续稳定运行时间仅不到1个月，蛋白质质量差，粗蛋白含量为65％-75％、灰分偏高8％-10％；蛋白质整体产率为80％-85％，且产量和蒸汽 消耗量是仅为实施例的50％左右。
本公开提供的一种含碳工业气体工业化蛋白质生产系统通过净化原料气和原料水，除去原料气中的杂质，为菌体生长繁殖提供优质原料，除去原料水中的杂菌，为菌体生长繁殖提供优良环境，进而使原料气在菌体制备系统3中进行高效的发酵，并通过菌体分离系统4和蛋白质制取系统5获得高质量的菌体蛋白质。其蛋白质质量高，粗蛋白含量≥85％，灰分≤1％；菌体制备系统3中菌体浓度3％-6％，产量大大提升；同时所述菌体蛋白质产率＞95％，蛋白质损耗降低；且所述菌体制备系统3连续稳定运行时间＞12个月，综合效率高。
需要说明的是，在本文中，诸如“第一”和“第二”等之类的关系术语仅仅用来将一个实体或者操作与另一个实体或操作区分开来，而不一定要求或者暗示这些实体或操作之间存在任何这种实际的关系或者顺序。而且，术语“包括”、“包含”或者其任何其他变体意在涵盖非排他性的包含，从而使得包括一系列要素的过程、方法、物品或者设备不仅包括那些要素，而且还包括没有明确列出的其他要素，或者是还包括为这种过程、方法、物品或者设备所固有的要素。在没有更多限制的情况下，由语句“包括一个……”限定的要素，并不排除在包括所述要素的过程、方法、物品或者设备中还存在另外的相同要素。
以上所述仅是本发明的具体实施方式，使本领域技术人员能够理解或实现本发明。对这些实施例的多种修改对本领域的技术人员来说将是显而易见的，本文中所定义的一般原理可以在不脱离本发明的精神或范围的情况下，在其它实施例中实现。因此，本发明将不会被限制于本文所示的这些实施例，而是要符合与本文所申请的原理和新颖特点相一致的最宽的范围。

Claims (10)

  1. 一种含碳工业气体工业化蛋白质生产系统，包括：
    原料气净化系统(1)，用以除去原料气中的杂质；
    水净化系统(2)，用以杀灭原料水中的杂菌；
    菌体制备系统(3)，与所述原料气净化系统(1)以及水净化系统(2)连通并且用以将原料气和原料水作为原料进行发酵，得到醪液；
    菌体分离系统(4)，与所述菌体制备系统(3)连通并且用以将所述醪液分离出菌体和醇；以及
    蛋白质制取系统(5)，与所述菌体分离系统(4)连通并且用以将菌体制成菌体蛋白质产品。
  2. 如权利要求1所述的含碳工业气体工业化蛋白质生产系统，其中，所述水净化系统(2)包括：
    气体灭菌装置(21)，与所述原料气净化系统(1)连通并且用以向原料水中通入灭菌气体；以及
    化学灭菌装置(22)，与所述气体灭菌装置(21)和所述菌体制备系统(3)连通并且用以向原料水中加入灭菌剂。
  3. 如权利要求1所述的含碳工业气体工业化蛋白质生产系统，其中，所述菌体分离系统(4)包括：
    蒸馏分离装置(41)，与所述菌体制备系统(3)连通并且用以分离所述醪液中的醇；以及
    离心分离装置(42)，与所述蒸馏分离装置(41)和所述蛋白质制取系统(5)连通并且用以分离醪液中的菌体。
  4. 如权利要求3所述的含碳工业气体工业化蛋白质生产系统，其中，所述蒸馏分离装置(41)底部的温度≤90℃，顶部的压力≤-50kPa。
  5. 如权利要求1所述的含碳工业气体工业化蛋白质生产系统，其中，所述原料气净化系统(1)包括：
    原料气除尘装置，用以除去原料气中的粉尘和焦油；以及
    原料气杂质净化装置，与所述原料气除尘装置连通并且用以除去原料气 中的苯系物、氧气和卤化物。
  6. 如权利要求5所述的含碳工业气体工业化蛋白质生产系统，其中，所述原料气净化系统(1)还包括：
    原料气温控装置(13)，与所述原料气杂质净化装置连通并且用以对原料气的温度进行控制；以及
    水分离器(14)，与所述原料气温控装置(13)以及菌体制备系统(3)连通并且用以除去原料气中的水分。
  7. 如权利要求1所述的含碳工业气体工业化蛋白质生产系统，其中，所述菌体制备系统(3)包括：
    菌体制备装置(31)，与所述原料气净化系统(1)以及水净化系统(2)连通并且用以在菌体的作用下以原料气和原料水为原料进行发酵，得到醪液；以及
    菌体输送泵(32)，与所述菌体制备装置(31)以及菌体分离系统(4)连通并且用以将所述菌体制备装置(31)内醪液泵入菌体分离系统(4)。
  8. 如权利要求7所述的含碳工业气体工业化蛋白质生产系统，其中，所述菌体制备系统(3)还包括：
    菌体提浓装置(33)，与所述菌体输送泵(32)以及菌体制备装置(31)连通并且用以将醪液分离为浓醪液和清醪液，并调节菌体制备装置(31)中的醪液浓度和液位。
  9. 如权利要求1所述的含碳工业气体工业化蛋白质生产系统，其中，所述蛋白质制取系统(5)包括：
    干燥装置(51)，与所述菌体分离系统(4)连通并且用以对菌体进行干燥，制备成菌体蛋白质；以及
    包装装置(52)，与所述干燥装置(51)连通并且用以将菌体蛋白质包装成菌体蛋白质产品。
  10. 如权利要求9所述的含碳工业气体工业化蛋白质生产系统，其中，所述干燥装置(51)干燥温度≤200℃。
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