Classifications

• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
  • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
  • A61K8/36—Carboxylic acids; Salts or anhydrides thereof
  • A61K8/361—Carboxylic acids having more than seven carbon atoms in an unbroken chain; Salts or anhydrides thereof
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
  • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
  • A61K8/34—Alcohols
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
  • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
  • A61K8/34—Alcohols
  • A61K8/345—Alcohols containing more than one hydroxy group
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
  • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
  • A61K8/36—Carboxylic acids; Salts or anhydrides thereof
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
  • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
  • A61K8/66—Enzymes
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
  • A61Q19/00—Preparations for care of the skin
  • A61Q19/10—Washing or bathing preparations
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
  • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
  • A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
  • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
  • A61K2800/87—Application Devices; Containers; Packaging

Definitions

• the present invention relates to cleaning compositions. More specifically, the present invention relates to a cleaning composition capable of suppressing a decrease in the activity of protease and lipid degrading enzyme and stably sustaining the activity of the degrading enzyme, and a method for producing the same.
• Patent Document 1 discloses a facial cleansing powder containing a proteolytic enzyme derived from microorganisms.
• Enzymes such as proteolytic enzymes lose their activity or become inactive when stored for a certain period of time in the presence of water.
• the water content of the cleaning composition should generally be very low.
• usability is problematic.
• the affinity between the cleaning composition and water tends to decrease
• the water solubility tends to deteriorate during use
• the dischargeability tends to decrease when filled in a tube-shaped container, resulting in usability problems.
• there is on the other hand in order to prevent deactivation of enzymes, for example, there is a product specification in which powdered facial cleanser is individually packaged for each use. There was also a lot of needs.
• the present invention provides a cleaning composition that can suppress the decrease in enzyme activity and has excellent usability.
• the present inventor surprisingly found that, together with proteolytic enzymes and lipolytic enzymes, higher fatty acids and/or salts thereof, specific polyhydric alcohols, and water were blended, It has been found that by controlling the content of the polyhydric alcohol and water within a specific range, it is possible to suppress the decrease in enzymatic activity of protease and lipid degrading enzyme, and to provide a cleaning composition excellent in usability.
• a cleaning composition containing (A) a higher fatty acid and/or a salt thereof, (B) a proteolytic enzyme and/or a lipolytic enzyme, (C) a polyhydric alcohol, and (D) water , the content of the above (C) is 25 to 60% by mass relative to the total amount of the cleaning composition, and the content of the above (D) is 5 to 30% by mass relative to the total amount of the cleaning composition.
• the numerical range described using “X to Y" means “X or more and Y or less” unless otherwise specified.
• the upper limit or lower limit of the numerical range in one step can be arbitrarily combined with the upper limit or lower limit of the numerical range in another step.
• the upper limit or lower limit of the numerical range can be replaced with the values shown in Examples or Formulation Examples.
• “ ⁇ and/or ⁇ " ( ⁇ and ⁇ are arbitrary constituents or components) in this specification are defined as “ ⁇ only", “ ⁇ only”, “Both ⁇ and ⁇ ” means a combination of streets.
• a cleaning composition according to an embodiment of the present invention contains (A) a higher fatty acid and/or a salt thereof, (B) a proteolytic enzyme and/or a lipolytic enzyme, (C) a polyhydric alcohol, and (D) water.
• the content of (C) is 25 to 60% by mass relative to the total amount of the cleaning composition, and the content of (D) is 5 to 30% by mass relative to the total amount of the cleaning composition.
• Component (A) used in the cleaning composition according to the embodiment of the present invention can be used without any particular limitation as long as it is a higher fatty acid and/or salt thereof that is usually used in external compositions and/or cosmetics. .
• the component (A) includes, for example, higher fatty acids having 10 or more carbon atoms and/or salts thereof.
• Specific examples of the component (A) include, but are not limited to, linear saturated acids such as capric acid, lauric acid, myristic acid, pentadecylic acid, palmitic acid, margaric acid, stearic acid, arachidic acid, and behenic acid.
• Fatty acids unsaturated fatty acids such as underesic acid, palmitoleic acid, oleic acid, linoleic acid, linolenic acid, vaccenic acid, elaidic acid, ricinoleic acid, petroselinic acid, arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid; isopalmitic acid, isostearic acid mixed fatty acids such as coconut oil fatty acid, beef tallow fatty acid and palm kernel oil fatty acid; 12-hydroxystearic acid and salts thereof; These can be used alone or in combination of two or more.
• unsaturated fatty acids such as underesic acid, palmitoleic acid, oleic acid, linoleic acid, linolenic acid, vaccenic acid, elaidic acid, ricinoleic acid, petroselinic acid, arachidonic acid, eicosapent
• Salts of higher fatty acids include, but are not limited to, metal salts such as potassium salts, calcium salts, lithium salts, sodium salts and magnesium salts; ammonium salts such as ammonium salts and alkylammonium salts; monoethanolamine salts; Amine salts such as diethanolamine salts, triethanolamine salts, aminomethylpropanol salts, and tromethamine salts are included.
• metal salts such as potassium salts, calcium salts, lithium salts, sodium salts and magnesium salts
• ammonium salts such as ammonium salts and alkylammonium salts
• monoethanolamine salts monoethanolamine salts
• Amine salts such as diethanolamine salts, triethanolamine salts, aminomethylpropanol salts, and tromethamine salts are included.
• component (A) one or more selected from the group consisting of higher fatty acids having 12 to 18 carbon atoms and salts thereof. preferably. From the viewpoint of exhibiting the effect of the present invention more remarkably, it is preferable to contain, as component (A), one or more selected from the group consisting of lauric acid, myristic acid, palmitic acid, stearic acid and salts thereof.
• component (A) it is also preferable to contain two or more selected from the group consisting of lauric acid, myristic acid, palmitic acid, stearic acid and salts thereof, such as lauric acid, myristic acid, palmitic acid and stearin. It is also preferred to contain three or more selected from the group consisting of acids and salts thereof.
• the higher fatty acid salt may be blended into the cleaning composition as a higher fatty acid salt, or the higher fatty acid and the base may be blended independently to form the higher fatty acid salt in the composition. .
• component (A) can be appropriately set according to the type and amount of other components, the dosage form, etc., and is not limited, but from the viewpoint of significantly exhibiting the effects of the present invention, it is For example, 10 to 40% by mass is preferable, 12 to 38% by mass is more preferable, 15 to 36% by mass is even more preferable, and 20 to 35% by mass is particularly preferable.
• the cleaning composition according to the embodiment of the present invention contains two or more selected from the group consisting of lauric acid, myristic acid, palmitic acid, stearic acid, and salts thereof as component (A), each of these
• the content of the component can be appropriately set according to the type and amount of the other component, the dosage form, etc., and is not particularly limited, but is, for example, as follows.
• the content of lauric acid and/or its salt is, for example, 1 to 35% by mass, 2 to 15% by mass, 3 to 12% by mass, 4 to 10% by mass, etc. relative to the total amount of the cleaning composition.
• the content of myristic acid and/or a salt thereof is, for example, 1 to 35% by mass, 2 to 15% by mass, 3 to 12% by mass, 4 to 10% by mass, etc. relative to the total amount of the cleaning composition.
• the content of palmitic acid and/or a salt thereof is, for example, 1 to 35% by mass, 3 to 30% by mass, 5 to 20% by mass, 8 to 18% by mass, etc. relative to the total amount of the cleaning composition.
• the content of stearic acid and/or a salt thereof is, for example, 1 to 35% by mass, 1.5 to 30% by mass, 2 to 20% by mass, 3 to 10% by mass, etc., relative to the total amount of the cleaning composition. .
• the component (B) used in the cleaning composition according to the embodiment of the present invention can be used without particular limitation as long as it is a proteolytic enzyme or lipolytic enzyme that is usually used in external compositions and/or cosmetics. . These can be used alone or in combination of two or more.
• the protease may be any of acidic, neutral, or alkaline protease, and may be any of animal-derived, plant-derived, or microbial-derived protease.
• Specific examples of proteases include plant-derived proteases such as papain, chymopapain, bromelain, ficin and pepsin, animal-derived proteases such as trypsin, chymotrypsin and pancreatin, and microorganisms such as bacteria. and proteolytic enzymes derived from microorganisms.
• Proteases derived from microorganisms include, for example, Bacillus, Paenibacillus, Geobacillus, Aspergillus, Rhizopus, Rhizomucor, and Streptomyces. and proteolytic enzymes produced by microorganisms of the genus Streptomyces. These can be used alone or in combination of two or more.
• a microorganism-derived protease and a plant-derived protease are preferable, and a protease produced by a microorganism belonging to the genus Bacillus is more preferable. preferable.
• the lipolytic enzyme may be an acidic, neutral, or alkaline lipolytic enzyme, or may be an animal-derived, plant-derived, or microbial-derived lipolytic enzyme.
• Specific examples of lipolytic enzymes include lipase, phospholipase, and phytase.
• lipolytic enzymes derived from microorganisms include Rhizopus, Aspergillus, Mucour, Geotrichum, Candida, Pseudomonas, and Penicillium. (Penicillium), lipolytic enzymes produced by microorganisms such as Chromobacterium. These can be used alone or in combination of two or more. Among these, lipolytic enzymes derived from microorganisms are preferable from the viewpoint of exhibiting the effects of the present invention remarkably.
• component (B) Commercially available products of component (B) include, for example, "Bioprase (registered trademark) [eg, Bioprase XL-416F]", "Protease [eg, Protease CL-15]” (manufactured by Nagase ChemteX Corporation), “ Protease N”, “Protease S”, “Papain W-40” (manufactured by Amano Pharmaceutical Co., Ltd.), “Biosork” (manufactured by Daiwa Kasei Co., Ltd.), “Alcalase (registered trademark)”, “Esperase (registered trademark)”, “Savinase (registered trademark)”, “Durazyme (registered trademark)”, “Subtilisin A” (manufactured by Novozymes Japan), “Relipase (registered trademark) [for example, Lilipase A-10D]” (Nagase ChemteX Corporation) made) and the like.
• component (B) can be appropriately set according to the type and amount of other components, the dosage form, etc., and is not limited. , preferably 0.01 to 5.0 U/g, more preferably 0.1 to 4.0 U/g, still more preferably 0.5 to 3.0 U/g, particularly preferably 1.0 to 2.0 U /g.
• "U (unit)" in the unit “U / g” indicates the activity of the protease or lipid degrading enzyme, and 1 unit is the optimum condition (temperature 30 ° C., the most chemical It is defined as the amount of enzyme (1 micromole per minute) that can convert 1 micromole ( ⁇ mol) of substrate per minute at the acidity at which the reaction proceeds.
• component (B) can be appropriately set according to its enzymatic activity (U/g), the type and amount of other components, the dosage form, etc., and is not limited, but is, for example, 0.01 to 5% by mass. , 0.05 to 3% by mass, 0.08 to 2% by mass, 0.1 to 1% by mass, and the like.
• Component (B) is in an immobilized state, i.e., an enzyme immobilized by a method such as being immobilized on a carrier, or in a non-immobilized state, i.e., immobilized by a method such as being immobilized on a carrier.
• any non-enzymatic enzyme examples include known carriers that are usually used for immobilizing enzymes. Examples thereof include organic polymer compounds such as various ion-exchange resins, and inorganic porous materials such as ceramics.
• a method for immobilizing the enzyme various known methods can be employed.
• entrapment immobilization method for example, entrapment immobilization method, inorganic carrier covalent bonding method, organic carrier covalent bonding method, physical adsorption method and the like can be mentioned.
• INDUSTRIAL APPLICABILITY The present invention is particularly useful in that it can effectively suppress a decrease in enzymatic activity even for non-immobilized enzymes whose enzymatic activity is likely to decrease.
• component (C) polyhydric alcohol As the component (C) used in the cleaning composition according to the embodiment of the present invention, a polyhydric alcohol usually used in external compositions and/or cosmetics can be used.
• the valence (number of hydroxyl groups) of component (C) is not particularly limited, but is, for example, 2 to 20 valence, preferably 2 to 10 valence, more preferably 2 to 6 valence, and still more preferably 2 to 4 valence. be.
• the molecular weight of component (C) is not particularly limited, but is, for example, 50-1000, preferably 50-600, more preferably 75-300.
• component (C) examples include glycerin, sorbitol, diglycerin, triglycerin, propylene glycol, dipropylene glycol, ethylene glycol, diethylene glycol, isoprene glycol, butylene glycol, propanediol, xylitol, erythritol, mannitol, and pentane. diol, hexanediol, octanediol, decanediol, neopentyl glycol, polyethylene glycol and the like.
• polyethylene glycol examples include polyethylene glycol having an average molecular weight of 150-1000. Specifically, polyethylene glycol 200 (PEG-4), polyethylene glycol 300 (PEG-6), polyethylene glycol 400 (PEG-8), polyethylene glycol 600 (PEG-12), polyethylene glycol 1000 (PEG-20), etc. is mentioned.
• the average molecular weight can be determined, for example, according to the average molecular weight test described in the section of Standards for Quasi-drug Ingredients 2006 Polyethylene Glycol.
• the cleaning composition according to the embodiment of the present invention contains glycerin as the component (C). Or it is necessary to contain sorbitol.
• the content of component (C) in the cleaning composition according to the embodiment of the present invention must be 25 to 60% by mass with respect to the total amount of the cleaning composition, from the viewpoint of achieving the effects of the present invention. Also, the content of glycerin and/or sorbitol should be 10 to 60% by mass with respect to the total amount of the cleaning composition.
• the content of glycerin and/or sorbitol is, for example, preferably 12 to 55% by mass, more preferably 15 to 50% by mass, more preferably 18 to 50% by mass, based on the total amount of the cleaning composition, from the viewpoint of significantly exhibiting the effects of the present invention. 45% by weight is even more preferred.
• the (C) component in the cleaning composition according to the embodiment of the present invention preferably contains glycerin from the viewpoint of further enhancing the effects of the present invention (in particular, the effect of increasing water solubility).
• the content of glycerin is, for example, preferably 12 to 50% by mass, preferably 14 to 45% by mass, based on the total amount of the cleaning composition, from the viewpoint of significantly exhibiting the effects of the present invention (in particular, the effect of increasing water solubility). is more preferred, and 16 to 35% by mass is even more preferred.
• the content of glycerin relative to the total amount of the component (C) is, for example, preferably 50% by mass or more, more preferably 60% by mass or more.
• the upper limit is not particularly limited, it is, for example, 99.8% by mass.
• the cleaning composition according to the embodiment of the present invention may contain only glycerin and/or sorbitol as the component (C), or glycerin and/or sorbitol and other polyhydric alcohols ( That is, polyhydric alcohols other than glycerin and sorbitol) may be used in combination.
• the other polyhydric alcohol is not particularly limited, but from the viewpoint of remarkably exhibiting the effects of the present invention, for example, diglycerin, triglycerin, propylene glycol, dipropylene glycol, ethylene glycol, diethylene glycol, isoprene glycol, butylene Glycol, and one or more selected from the group consisting of polyethylene glycol having an average molecular weight of 150 to 1000 (more preferably an average molecular weight of 150 to 600, still more preferably 150 to 300), diglycerin , dipropylene glycol, and butylene glycol.
• diglycerin, triglycerin, propylene glycol, dipropylene glycol, ethylene glycol, diethylene glycol, isoprene glycol, butylene Glycol and one or more selected from the group consisting of polyethylene glycol having an average molecular weight of 150 to 1000 (more preferably an average molecular weight of 150 to 600, still more preferably 150 to 300), diglycerin
• one or more selected from the group consisting of diglycerin, dipropylene glycol, and butylene glycol, and glycerin is even more preferable to use together.
• the content of the other polyhydric alcohol is 0 to 50% by mass with respect to the total amount of the cleaning composition.
• the content of the other polyhydric alcohol can be appropriately set within the above range, and is not limited, for example, 5 to 40% by mass, 8 to 30% by mass, 10 to 20% by mass, etc. There may be.
• the total content of component (A) and component (C) [(A) + (C)] can be appropriately set according to the type and amount of other components, the dosage form, etc., and is not limited, but the content of the present invention is not limited. From the viewpoint of exhibiting a remarkable effect, for example, 40 to 90% by mass is preferable, 45 to 80% by mass is more preferable, and 50 to 75% by mass is even more preferable, relative to the total amount of the cleaning composition.
• Component (D) used in the cleaning composition according to the embodiment of the present invention can be used without particular limitation as long as it is water that is usually used in external compositions and/or cosmetics.
• purified water is preferably used.
• the content of component (D) must be 5 to 30% by mass relative to the total amount of the cleaning composition.
• the content of component (D) is preferably, for example, 10 to 30% by mass relative to the total amount of the cleaning composition. , more preferably 15 to 30% by mass, and even more preferably 20 to 30% by mass.
• pH adjuster In the cleaning composition according to the embodiment of the present invention, it is preferable to use (E) a pH adjuster in addition to the above components (A) to (D).
• pH adjusters include, but are not limited to, inorganic bases such as potassium hydroxide, sodium hydroxide and sodium carbonate; organic bases such as triethanolamine, diisopropanolamine and triisopropanolamine; inorganic bases such as hydrochloric acid and sulfuric acid; Acids, lactic acid, sodium lactate, citric acid, sodium citrate, succinic acid, organic acids such as sodium succinate, and the like. These can be used alone or in combination of two or more. Among these, potassium hydroxide and sodium hydroxide are preferred.
• component (E) can be appropriately set according to the type and amount of other components, the dosage form, etc., and is not limited, but is, for example, 0.1 to 10% by mass, based on the total amount of the cleaning composition. 0.3 to 8% by mass, 0.5 to 7% by mass, and the like.
• the cleaning composition according to the embodiment of the present invention may contain components other than the above components (A) to (E) as long as the effects of the present invention are not impaired.
• the cleaning composition according to the embodiment of the present invention includes, but is not limited to, bases, surfactants, thickeners, preservatives/preservatives, antioxidants, chelating agents, coloring agents, perfumes, and the like. can be compounded. These can be used alone or in combination of two or more.
• bases include lower alcohols such as ethanol and isopropanol; Polymerized methylpolysiloxane, cyclic silicone, alkyl-modified silicone, crosslinked alkyl-modified silicone, amino-modified silicone, polyether-modified silicone, polyglycerin-modified silicone, crosslinked polyether-modified silicone, crosslinked alkylpolyether-modified silicone, silicone alkyl Chain co-modified polyether-modified silicone, silicone/alkyl chain co-modified polyglycerin-modified silicone, polyether-modified branched silicone, polyglycerin-modified branched silicone, acrylic silicone, phenyl-modified silicone, silicone oils such as silicone resin; coconut oil, olive oil, Fats such as rice bran oil, shea butter, rosehip oil, and almond oil; Waxes such as jojoba oil, miura, candelilla wax, and lanolin; Cetanol, cetostearyl alcohol, stearyl alcohol, behenyl alcohol, ste
• the content of the base can be appropriately set according to the type and amount of other components, the dosage form, etc., and is not limited, but is, for example, 1 to 30% by mass, preferably 1 to 30% by mass, based on the total amount of the cleaning composition. 3 to 25% by mass, more preferably 5 to 20% by mass.
• Surfactants include, for example, nonionic surfactants, anionic surfactants, cationic surfactants, amphoteric surfactants, and naturally derived surfactants. .
• the content of the surfactant can be appropriately set according to the type and amount of other components, the dosage form, etc., and is not limited, but is, for example, 1 to 30% by mass, 5 to 25% by mass, based on the total amount of the cleaning composition. % by mass, 10 to 20% by mass, and the like.
• nonionic surfactants include sorbitan monoisostearate, sorbitan monolaurate, sorbitan monopalmitate, sorbitan monostearate, diglycerol sorbitan penta-2-ethylhexylate, and diglycerol sorbitan tetra-2-ethylhexylate.
• sorbitan fatty acid esters such as; lipophilic glyceryl monostearate, self-emulsifying glyceryl monostearate, glycerin fatty acid esters such as glyceryl monostearate malic acid; polyglyceryl monostearate, polyglyceryl monoisostearate, polyglyceryl diisostearate, Polyglycerin fatty acid esters such as polyglyceryl monolaurate, polyglyceryl monooleate, polyglyceryl monomyristate; propylene glycol fatty acid esters such as propylene glycol monostearate; ethylene glycol distearate, ethylene glycol monostearate, triethylene glycol distearate , polyethylene glycol monostearate (2E.O.), polyethylene glycol monostearate (4E.O.), polyethylene glycol monostearate (9E.O.), polyethylene glycol monostearate (10E.O.), monostearate Polyethylene G
• glycerin fatty acid esters, ethylene glycol fatty acid esters, and alkyl glucosides are preferable, and self-emulsifying glyceryl monostearate, ethylene glycol distearate, and polyethylene glycol monostearate (the average number of added moles of ethylene oxide is 2 to 2).
• 400, preferably 10 to 300, more preferably 100 to 150), polyethylene glycol distearate (average added mole number of ethylene oxide is 2 to 400, preferably 100 to 300), and lauryl glucoside are more preferred.
• the HLB value (Hydrophile-Lipophile Balance value) of the nonionic surfactant is not particularly limited, but is, for example, 8 to 19, preferably 9 to 18, and 9.5 from the viewpoint of exhibiting the effects of the present invention remarkably. ⁇ 17 is more preferred.
• the HLB value is an index showing the hydrophilic-lipophilic balance, and is calculated, for example, by the following (formula 1) by Oda and Teramura et al.
• HLB value ( ⁇ inorganic value/ ⁇ organic value) ⁇ 10 (Formula 1)
• the ⁇ inorganic value/ ⁇ organic value is called IOB (Inorganic-Organic Balance), and based on the "inorganic value” and “organic value” set for each atom and functional group, surfactants, etc.
• the content of the nonionic surfactant can be appropriately set according to the type and amount of other components, the dosage form, etc., and is not limited, but is, for example, 1 to 20% by mass, 1 to 20% by mass, 1 .5 to 15% by mass, 2 to 12% by mass, 2.5 to 10% by mass, 2.5 to 8% by mass, etc. are preferred.
• anionic surfactants include alkyl sulfate salts such as sodium lauryl sulfate, triethanolamine lauryl sulfate, sodium laureth sulfate, sodium myristyl sulfate, sodium stearyl sulfate, sodium oleyl sulfate, and sodium cetyl sulfate; polyoxyethylene lauryl Polyoxyethylene alkyl ether sulfates such as sodium ether sulfate and sodium polyoxyethylene myristyl ether sulfate; N-acyl methyl taurine salts; alkyl sulfosuccinates such as dioctyl sodium sulfosuccinate and disodium lauryl sulfosuccinate; polyoxyethylene alkyl sulfosuccinates such as polyoxyethylene lauryl disodium sulfosuccinate; sodium lauryl phosphate, sodium cetyl phosphate , monoalky
• Cationic surfactants include, for example, stearyltrimethylammonium chloride, alkyltrimethylammonium chloride, cetyltrimethylammonium chloride, behenyltrimethylammonium chloride, behenyldimethylhydroxyethylammonium chloride, stearyldimethylbenzylammonium chloride, distearyldimethylammonium chloride, chloride dicetylmethylammonium, cetyltriethylammonium methylsulfate, stearyltrimethylammonium bromide, cetyltrimethylammonium bromide and the like.
• amphoteric surfactants include lauryl hydroxysulfobetaine, lauramidopropyl betaine, 2-alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine, lauryldimethylaminoacetic acid betaine, N-lauroyl-N'. -carboxymethyl-N'-hydroxyethylethylenediamine sodium, lauramidopropyl hydroxysultaine, lauramidopropyl dimethylamine oxide, hydroxyalkyl (C12-14) hydroxyethyl sarcosine, coconut oil fatty acid amidopropyl betaine and the like.
• Naturally derived surfactants include lecithin, hydrogenated lecithin, saponin, surfactin sodium, and bile acid.
• Thickeners include, for example, gums (gellan gum, xanthan gum, sclerotium gum, locust bean gum, biosaccharide gum, tamarind gum, quince seed, gum arabic, tara gum, guar gum, galactan, arabic gum, tragacanth gum, etc.), Carrageenan, curdlan, succinoglucan, heparinoids, alginic acids (alginic acid, sodium alginate, propylene glycol alginate, etc.), agar (including agarose), gelatin, pectin, pullulan, mannan, vinyl thickener (polyvinyl alcohol) , polyvinylpyrrolidone, polyvinyl methyl ether, carboxyvinyl polymer, etc.), cellulose-based thickeners (methylcellulose, ethylcellulose, hydroxyethylcellulose, hydroxymethylcellulose, hydroxypropylcellulose, hydroxypropylmethylcellulose, carboxymethylcellulose, carboxyethy
• the content of the thickening agent can be appropriately set according to the type and amount of other components, the dosage form, etc., and is not limited, but is, for example, 0.001 to 5% by mass with respect to the total amount of the cleaning composition. , preferably 0.01 to 3% by mass, more preferably 0.05 to 1% by mass.
• antiseptics/preservatives examples include isopropylmethylphenol, chlorobutanol, benzyl alcohol, phenethyl alcohol, benzoic acid, sodium benzoate, dehydroacetic acid, sodium dehydroacetate, isobutyl parahydroxybenzoate, isopropyl parahydroxybenzoate, and parahydroxybenzoic acid.
• the content of the antiseptic/preservative can be appropriately set according to the type and amount of other ingredients, the dosage form, etc., and is not limited, but is, for example, 0.001 to 2.5 with respect to the total amount of the cleaning composition. % by mass, preferably 0.005 to 2% by mass, more preferably 0.01 to 1% by mass.
• antioxidants examples include dibutylhydroxytoluene (BHT), butylhydroxyanisole, sorbic acid, sodium pyrosulfite, erythorbic acid, and L-cysteine hydrochloride.
• BHT dibutylhydroxytoluene
• sorbic acid sodium pyrosulfite
• erythorbic acid and L-cysteine hydrochloride.
• the content of the antioxidant can be appropriately set according to the type and amount of other components, the dosage form, etc., and is not limited, but is, for example, 0.001 to 2.5% by mass with respect to the total amount of the cleaning composition. , preferably 0.005 to 2% by mass, more preferably 0.01 to 1% by mass.
• the chelating agent examples include EDTA.disodium salt, EDTA.calcium.disodium salt, and the like.
• the content of the chelating agent can be appropriately set according to the type and amount of other components, the dosage form, etc., and is not limited, but is, for example, 0.001 to 2.5% by mass with respect to the total amount of the cleaning composition. Yes, preferably 0.005 to 2% by mass, more preferably 0.01 to 1% by mass.
• coloring agents include inorganic pigments and natural pigments.
• the content of the coloring agent can be appropriately set according to the type and amount of other components, the dosage form, etc., and is not limited, but is, for example, 0.001 to 1% by mass with respect to the total amount of the cleaning composition. It is preferably 0.005 to 0.1% by mass, more preferably 0.01 to 0.05% by mass.
• Perfumes include, for example, terpenoids such as menthol, camphor, borneol, geraniol, cineol, anethole, limonene, and eugenol.
• the content of the perfume can be appropriately set according to the type and amount of other components, the dosage form, etc., and is not limited, but is, for example, 0.001 to 2.5% by mass with respect to the total amount of the cleaning composition. , preferably 0.005 to 2% by mass, more preferably 0.01 to 1% by mass.
• the cleaning composition according to the embodiment of the present invention can contain other active ingredients within a range that does not impair the effects of the present invention.
• active ingredients include, but are not limited to, moisturizing ingredients, scrubbing agents, blood circulation promoters, astringent ingredients, ultraviolet absorbing ingredients, antibacterial ingredients, anti-inflammatory agents, vitamins, peptides or derivatives thereof, amino acids or Derivatives thereof, cell activation components, and the like are included. These can be used alone or in combination of two or more.
• moisturizing ingredients include natural moisturizing factors such as sodium lactate, urea, and sodium pyrrolidonecarboxylate, chamomile extract, hamamelis extract, tea extract, perilla extract, coix seed extract, centella asiatica leaf extract, seaweed extract, loquat leaf extract, corn Plant extracts such as free extract, bilberry leaf extract, coral grass extract, white willow bark extract, rosemary extract, soybean extract, soybean sprout extract, chimp extract, orange extract, grapefruit extract, lemon extract, saxifrage extract, edelweiss extract, etc. mentioned.
• natural moisturizing factors such as sodium lactate, urea, and sodium pyrrolidonecarboxylate, chamomile extract, hamamelis extract, tea extract, perilla extract, coix seed extract, centella asiatica leaf extract, seaweed extract, loquat leaf extract, corn Plant extracts such as free extract, bilberry leaf extract, coral grass extract, white willow bark extract, rosemary extract, soybean extract, soybean sprout extract, chimp extract
• the content of the moisturizing ingredient can be appropriately set according to the type and amount of other ingredients, the dosage form, etc., and is not limited, but is, for example, 0.001 to 20% by mass with respect to the total amount of the cleaning composition. It is preferably 0.01 to 15% by mass, more preferably 0.05 to 10% by mass.
• Examples of scrub agents include apricot kernel powder, almond shell powder, apricot kernel powder, sodium chloride grains, olive kernel powder, dried seawater grains, candelilla wax, walnut shell powder, cherry kernel powder, coral powder, charcoal powder ( paulownia charcoal, binchotan charcoal, bamboo charcoal, cypress charcoal, coconut shell charcoal and their activated charcoal, and their medicinal charcoal), hawthorn husk powder, polyethylene powder, anhydrous silicic acid, kaolin and the like.
• the content of the scrubbing agent can be appropriately set according to the type and amount of other components, the dosage form, etc., and is not limited, but is, for example, 0.01 to 15% by mass with respect to the total amount of the cleaning composition. It is preferably 0.05 to 10% by mass, more preferably 0.1 to 5% by mass.
• Blood circulation promoters include, for example, acetylcholine, ictamol, caffeine, capsaicin, cantharis tincture, ⁇ -oryzanol, ginger tincture, gingerone, cepharanthine, jersey extract, tannic acid, capsicum tincture, tolazoline, tocopherol nicotinate, and nicotinic acid. benzyl ester and the like.
• the content of the blood circulation promoting agent can be appropriately set according to the type and amount of other ingredients, the dosage form, etc., and is not limited, but is, for example, 0.001 to 5% by mass with respect to the total amount of the cleaning composition. , preferably 0.005 to 3% by mass, more preferably 0.01 to 1% by mass.
• Astringents include, for example, zinc sulfate, aluminum hydroxy, aluminum chloride, zinc sulfonate, and tannic acid.
• the content of the astringent component can be appropriately set according to the type and amount of other components, the dosage form, etc., and is not limited, but is, for example, 0.001 to 20% by mass with respect to the total amount of the cleaning composition. It is preferably 0.01 to 15% by mass, more preferably 0.1 to 10% by mass.
• UV-absorbing components include octyltriazone, diethylaminohydroxybenzoylhexylbenzoate, dimethoxybenzylidenedioxoimidazolidine octylpropionate, 2-ethylhexyl paramethoxycinnamate, t-butylmethoxydibenzoylmethane, and phenylbenzimidazole sulfone. acid, octyl methoxycinnamate, ethylhexyl methoxycinnamate, and the like.
• the content of the UV-absorbing component can be appropriately set according to the type and amount of other components, the dosage form, etc., and is not limited, but is preferably, for example, 1 to 30% by mass with respect to the total amount of the cleaning composition. is 3 to 25% by mass, more preferably 5 to 20% by mass.
• antibacterial components include isopropylmethylphenol, chlorhexidine, benzalkonium chloride, acrinol, benzethonium chloride, cresol, gluconic acid and its derivatives, povidone iodine, potassium iodide, iodine, triclocarban, triclosan, photosensitizer No. 101, photosensitizer. 201, paraben, phenoxyethanol, alkyldiaminoglycine hydrochloride, pyroctoolamine, miconazole and the like.
• the content of the antibacterial component can be appropriately set according to the type and amount of other components, dosage form, etc., and is not limited, but is, for example, 0.001 to 2.5% by mass with respect to the total amount of the cleaning composition. Yes, preferably 0.005 to 2% by mass, more preferably 0.01 to 1% by mass.
• Anti-inflammatory agents include, for example, non-steroidal anti-inflammatory agents, steroidal anti-inflammatory agents, and the like. Specifically, glycyrrhizic acid, glycyrrhetinic acid, stearyl glycyrrhetinate, allantoin, epsilon aminocaproic acid, salicylic acid, methyl salicylate, glycol salicylate, indomethacin, felbinac, ibuprofen, ibuprofen piconol, ketoprofen, bufexamac, butyl flufenamate, bendazac, piroxicam, suprofen, azulene, guaiazulene, dexamethasone valerate acetate, dexamethasone, prodnisolone valerate acetate (prednisolone valerate acetate), prodnisolone acetate, prodnisolone, hydrocortisone a
• the content of the anti-inflammatory agent can be appropriately set according to the type and amount of other ingredients, the dosage form, etc., and is not limited, but is, for example, 0.001 to 5% by mass with respect to the total amount of the cleaning composition. , preferably 0.005 to 4.5% by mass, more preferably 0.01 to 3% by mass.
• vitamins include vitamin E such as dl- ⁇ -tocopherol, d- ⁇ -tocopherol, dl- ⁇ -tocopherol succinate, dl- ⁇ -tocopherol calcium succinate, riboflavin, flavin mononucleotide, and flavin adenine.
• vitamin B2s such as riboflavin butyrate, riboflavin tetrabutyrate, riboflavin 5'-phosphate sodium, riboflavin tetranicotinate, dl- ⁇ -tocopherol nicotinate, benzyl nicotinate, methyl nicotinate, beta nicotinate -butoxyethyl, nicotinic acids such as 1-(4-methylphenyl)ethyl nicotinate, ascorbogen-A, L-ascorbic acid 2-glucoside, ascorbyl stearate, ascorbyl palmitate, L-ascorbyl dipalmitate vitamin Cs such as vitamin C, methylhesperidin, ergocalciferol, cholecalciferol, etc.
• vitamin Cs such as vitamin C, methylhesperidin, ergocalciferol, cholecalciferol, etc.
• vitamin Ds vitamin Ds, phylloquinone, farnoquinone, etc. vitamin Ks, ⁇ -oryzanol, dibenzoylthiamine, dibenzoylthiamine hydrochloride, thiamine hydrochloride, thiamine Cetyl hydrochloride, thiamine thiocyanate, thiamine lauryl hydrochloride, thiamine nitrate, thiamine monophosphate, thiamine lysine salt, thiamine triphosphate, thiamine monophosphate phosphate, thiamine monophosphate, thiamine diphosphate, thiamine vitamin B1s such as diphosphate hydrochloride, thiamine triphosphate, thiamine triphosphate monophosphate; vitamin B6s such as pyridoxine hydrochloride, pyridoxine acetate, pyridoxal hydrochloride, pyridoxal 5'-phosphate, pyridoxamine hydrochloride; Vitamin B12 such as hydroxocobalamin and de
• the content of vitamins can be appropriately set according to the type and amount of other ingredients, the dosage form, etc., and is not limited, but is, for example, 0.001 to 25% by mass with respect to the total amount of the cleaning composition. It is preferably 0.01 to 20% by mass, more preferably 0.05 to 15% by mass.
• Peptides or derivatives thereof include, for example, keratin-degrading peptides, hydrolyzed keratin, collagen, fish-derived collagen, atelocollagen, gelatin, elastin, elastin-degrading peptides, collagen-degrading peptides, hydrolyzed collagen, hydroxypropylammonium chloride hydrolyzed collagen, elastin Hydrolyzed peptide, conchiolin-degrading peptide, hydrolyzed conchiolin, silk proteolytic peptide, hydrolyzed silk, lauroyl hydrolyzed silk sodium, soybean proteolytic peptide, hydrolyzed soybean protein, wheat protein, wheat proteolytic peptide, hydrolyzed wheat protein, casein Examples include degraded peptides, acylated peptides (palmitoyl oligopeptide, palmitoyl pentapeptide, palmitoyl tetrapeptide, etc.).
• the content of the peptide or derivative thereof can be appropriately set according to the type and amount of other components, the dosage form, etc., and is not limited, but is, for example, 0.001 to 5% by mass with respect to the total amount of the cleaning composition. Yes, preferably 0.01 to 3% by mass, more preferably 0.05 to 1% by mass.
• amino acids or derivatives thereof include betaine (trimethylglycine), proline, hydroxyproline, arginine, lysine, serine, glycine, alanine, phenylalanine, ⁇ -alanine, threonine, glutamic acid, glutamine, asparagine, aspartic acid, cysteine, cystine. , methionine, leucine, isoleucine, valine, histidine, taurine, ⁇ -aminobutyric acid, ⁇ -amino- ⁇ -hydroxybutyric acid, carnitine, carnosine, creatine and the like.
• the content of the amino acid or derivative thereof can be appropriately set according to the type and amount of other ingredients, the dosage form, etc., and is not limited, but is, for example, 0.001 to 5% by mass with respect to the total amount of the cleaning composition. Yes, preferably 0.01 to 3% by mass, more preferably 0.05 to 1% by mass.
• cell activating components include ⁇ -hydroxy acids such as glycolic acid and lactic acid, tannins, flavonoids, saponins, photosensitizer No. 301, and the like.
• the content of the cell activating component can be appropriately set according to the type and amount of other components, the dosage form, etc., and is not limited, but is, for example, 0.001 to 5% by mass with respect to the total amount of the cleaning composition. Yes, preferably 0.01 to 3% by mass, more preferably 0.05 to 1% by mass.
• the pH of the cleaning composition according to the embodiment of the present invention can be appropriately set according to the dosage form and the like, and is not limited, but is preferably pH 8.0 to 12.0, for example. Further, the pH of the cleaning composition according to the embodiment of the present invention is more preferably pH 9.0 to 11.5, and even more preferably pH 9.0 to 11.0, from the viewpoint of suppressing a decrease in enzyme activity. .
• the above pH is measured at room temperature (23° C.) using a pH meter (for example, pH METER F-52 (manufactured by HORIBA)).
• the viscosity (25 ° C.) of the cleaning composition according to the embodiment of the present invention can be appropriately set according to the dosage form etc., and is not limited, but it is easy to discharge when filled into a tube-shaped container, and it can be easily discharged from the fingertips.
• 30,000 to 2,000,000 mPa s is preferable, 60,000 to 1,800,000 mPa s is more preferable, and 100,000 to 1,500,000 mPa is more preferable from the viewpoint that it is easy to lather without dripping from the palm.
• ⁇ s is more preferred, 150,000 to 1,300,000 mPa ⁇ s is even more preferred, and 150,000 to 1,000,000 mPa ⁇ s is most preferred.
• the above viscosity refers to the viscosity measured with a single cylindrical rotational viscometer (Brookfield viscometer) in accordance with the viscosity measurement method described in the general test method of the 17th revision of the Japanese Pharmacopoeia. Specifically, it refers to the value measured using the TV-10M (manufactured by Toki Sangyo Co., Ltd.). Measure the viscosity. More specifically, the viscosity at 25° C. is measured using an M4 rotor under conditions of a rotation speed of 0.3 rpm and a measurement time of 180 seconds.
• a description of a single cylindrical rotational viscometer is provided below.
• a single cylinder rotational viscometer is a viscometer that measures the torque when a cylinder in a liquid is rotated at a constant angular velocity.
• ⁇ Utility of the present invention Since proteolytic enzymes and lipid-degrading enzymes lose their enzymatic activity when stored in the presence of water for a certain period of time, they are usually included in the cleaning composition in order to maintain their enzymatic activity.
• the water content should be very low. When the water content in the cleaning composition is extremely low, the affinity between the cleaning composition and water tends to decrease, and the water solubility tends to deteriorate during use, or the cleaning composition itself becomes hard. There are cases where the usability is deteriorated, for example, there is a tendency for the dischargeability to decrease when filled into a tube-type container because of the excessive amount.
• the cleaning composition according to the embodiment of the present invention can suppress the decrease in enzymatic activity of protease and lipid degrading enzyme and stably maintain the enzymatic activity. Moreover, it is useful in that it can provide a cleaning composition with excellent usability.
• the cleaning composition according to the embodiment of the present invention comprises a proteolytic enzyme or a lipolytic enzyme, a higher fatty acid and/or a salt thereof, a specific polyhydric alcohol, and water.
• the cleaning composition according to the embodiment of the present invention can maintain the enzymatic activity of proteolytic enzymes and lipid-degrading enzymes even after a predetermined period of time has passed after production. It is excellent in cleaning effects such as removal effect and sebum removal effect.
• the cleaning composition according to the embodiment of the present invention has excellent usability, for example, because it has good water-solubility at the time of use.
• a preferred embodiment of the present invention includes, for example, setting the pH of the cleaning composition to around the optimum pH of the component (B).
• the cleansing effect such as exfoliating effect and sebum-removing effect becomes even more excellent.
• the vicinity of the optimum pH of the component (B) means that the pH of the cleaning composition is ⁇ 2.0, preferably ⁇ 1.5, and still more preferably ⁇ 1.5 of the optimum pH of the component (B). means 0.
• the optimum pH of the component (B) means the pH at which the enzymatic activity is maximized, and can be measured by a known method.
• component (B) eg, Bioprase (registered trademark) XL-416F (manufactured by Nagase ChemteX Corporation)
• a pH adjuster is added. is used to adjust the pH between 4.0 and 12.0, and the pH at which the relative activity of the enzyme is maximized when reacted at 30° C. for 10 minutes can be determined as the optimum pH.
• alkaline protease alkaline protease
• alkaline lipase alkaline lipid degrading enzyme
• component (B) alkaline lipase
• the alkaline protease means a protease having an optimum pH in the alkaline region, for example, a protease having an optimum pH of 9.0 or higher.
• an alkaline lipolytic enzyme means a lipolytic enzyme whose optimum pH is in the alkaline range, for example, a lipolytic enzyme whose optimum pH is 9.0 or higher.
• an alkaline proteolytic enzyme and/or an alkaline lipolytic enzyme having an optimum pH of 9.0 to 11.0 is used, and the pH of the cleaning composition is adjusted to 9.0 to 11.0. setting.
• the effect of suppressing the decrease in enzymatic activity of protease and lipid degrading enzyme is determined by, for example, enzymatic activity measured immediately after preparation of the cleaning composition and after storage for a predetermined period of time. It can be evaluated by comparing with the enzyme activity measured in. As a specific example, it can be evaluated by the method described in Examples below.
• the cleaning composition according to the embodiment of the present invention preferably has a residual enzyme activity rate of 70% or more, preferably 80% or more, as determined by the method described in Examples below or a method equivalent thereto. is more preferable, and 90% or more is even more preferable.
• the cleaning composition according to the embodiment of the present invention is prepared in various forms according to conventional methods by blending the above components and, if necessary, further components.
• the dosage form is not particularly limited, and may be any dosage form such as body soap, hand soap, face wash, shampoo, and the like. Further, it may be in the form of cleansing, rinse-in shampoo, conditioner, and the like. Also, the cleaning composition according to the embodiment of the present invention may be in any shape such as gel or paste.
• the cleaning composition according to the embodiment of the present invention is suitably used as a skin cleaning composition and a hair cleaning composition.
• the cleaning composition according to the embodiment of the present invention is useful for removing body fluids such as oil and sweat from the skin (including the scalp), dirt, and the like. It can be used particularly preferably as a composition (face wash).
• the cleaning composition and water are combined, foamed with the palm, and applied to the skin or the like.
• the cleaning composition according to the embodiment of the present invention can be used after being filled in an appropriately selected container according to the purpose and application.
• the container include bottle type, tube type, jar type, dispenser type, pouch bag, and chia pack.
• Materials constituting the container include, for example, polyethylene terephthalate, polypropylene, polyethylene (HDPE, LDPE, LLDPE, etc.), ABS resin, ethylene vinyl alcohol resin, polystyrene, glass, metals (aluminum, etc.), and mixtures thereof. is mentioned.
• containers molded from these materials can be subjected to various coating treatments, for example, in consideration of strength, flexibility, weather resistance, stability of components, and the like.
• the container can also be formed by laminating films or the like formed from the above materials.
• a container that can be used by pushing out the contents (cleaning composition) of the container by gripping the container with a user's hand and applying pressure is preferable, for example, a tube-shaped container. or bottle-shaped containers are preferred. Among them, a tube-shaped container is particularly preferable from the viewpoint of exhibiting the effects of the present invention remarkably.
• a known tube-type container can be used (for example, although not limited, JP-A-2000-272643, JP-A-2004-148628, JP-A-2005-022682, etc. reference).
• a specific example of the tube-shaped container includes a container having a main body for containing the cleaning composition and a cap.
• the main body is formed into a cylindrical shape by rolling a single layer or laminated sheet made of synthetic resin such as polyethylene (the total thickness of the sheet is, for example, about 0.1 to 1 mm), overlapping both ends, and heat-sealing them. a bottom seal portion formed by flatly heat-sealing one end of the body; and a head provided at the other end of the body.
• the head portion has a shoulder portion and a discharge port provided substantially in the center of the shoulder portion, and the discharge port is covered with a cap so as to be openable and closable.
• the user can push out the contents (cleaning composition) of the container by gripping the trunk and applying pressure, so that a cleaning composition with excellent usability can be provided. Extremely useful.
• the cleaning composition packed in a tube-shaped container is excellent in terms of barrier properties and sanitation, and is lightweight and excellent in portability, and is extremely useful from these points of view.
• Another embodiment of the present invention is preferably provided as a method for suppressing a decrease in enzymatic activity.
• A higher fatty acids and/or salts thereof,
• B protease and/or lipid degrading enzymes,
• C polyhydric alcohols, and
• D water
• the content of the polyhydric alcohol (C) is adjusted to 25 to 60% by mass relative to the total amount of the cleaning composition, and the content of glycerin and/or sorbitol as (C) is added to the total amount of the cleaning composition.
• (D) adjusting the water content to 5 to 30% by mass based on the total amount of the cleaning composition as a method for suppressing a decrease in enzyme activity. preferably provided.
• the method for producing the cleaning composition according to the embodiment of the present invention is not particularly limited, and it can be produced by a conventional method. For example, it can be produced by adding (A) to (D) and, if necessary, other components, and mixing them.
• phase II containing (A) heated and dissolved at 60 to 80 ° C. is added to phase I containing (C) and (D) heated to 60 ° C. or higher, stirred, and then cooled.
• a manufacturing method having a step of adding (B) at 55° C. or lower through the steps is preferred.
• phase I is prepared by heating (C) and (D) to 60°C or higher.
• the heating temperature can be appropriately set in the range of 60°C or higher, for example, preferably 60 to 85°C, more preferably 70 to 80°C.
• Phase I may contain components other than (C) and (D).
• phase II is prepared by heating and melting (A).
• the heating temperature can be appropriately set in the range of 60 to 80.degree. C., preferably 70 to 80.degree.
• Phase II may contain components other than (A).
• Phase II to Phase I obtained above, stir, cool the resulting mixture, and then add (B). Specifically, for example, while phase I is being stirred, phase II is gradually added and stirred, the resulting mixture is cooled to 55° C. or less, and then (B) is added while stirring the mixture. do.
• the temperature after cooling can be appropriately set in the range of 55°C or less, but from the viewpoint of exhibiting the effects of the present invention remarkably, it is preferably 30 to 55°C, more preferably 32 to 45°C.
• the mixture is agitated until uniform to obtain a cleaning composition according to embodiments of the present invention.
• the cleaning compositions (Examples 1 to 9 and Comparative Examples 1 to 5) having the formulations shown in Tables 1 and 2 below were prepared by a conventional method, and the enzymatic activity and usability were evaluated as follows.
• Example 1 50 ⁇ L of the cleaning composition of Example 1 is added to 100 ⁇ L of the quantification buffer, mixed, left to stand at 25° C. for 20 minutes, and then 50 ⁇ L of TNBSA diluted standard solution is added and mixed. Then, it was allowed to stand again at 25° C. for 20 minutes to prepare a control sample.
• the absorbance (A CT ) at 450 nm immediately after preparation of the control sample was measured using an absorbance meter (SH-9000Lab, manufactured by Corona Denki Co., Ltd.).
• 50 ⁇ L of the cleaning composition of Example 1 was added to 100 ⁇ L of the above succinylated casein solution, mixed, and allowed to stand at 25° C. for 20 minutes. After standing again at 25° C.
• Example 1 The absorbance at 450 nm (A1) immediately after preparation of the measurement sample and the absorbance (A2) at 450 nm after storage at 40° C. for one month were measured using an absorbance measuring device (SH-9000Lab, manufactured by Corona Denki Co., Ltd.). It was measured.
• the enzyme activity residual rate of Example 1 was obtained based on the following formula and evaluated based on the following criteria. Table 1 shows the results.
• Enzyme activity residual rate (%) [A2-A CT ]/[A1-A CT ] ⁇ 100 (Evaluation criteria) ⁇ (excellent): Enzyme activity residual rate is 90% or more ⁇ (very good): Enzyme activity residual rate is 70% or more and less than 90% ⁇ (good): Enzyme activity residual rate is 50% % or more and less than 70% ⁇ (poor) ⁇ Enzyme activity residual rate is less than 50%
• ⁇ Usability evaluation test (water solubility)> 1 g of the cleaning composition of Example 1 is placed in a 20 mL beaker, followed by 10 g of water (23° C.). A rotor made of tetrafluoroethylene resin (diameter 5 mm, total length 15 mm, manufactured by AS ONE) was put in, and a magnetic stirrer (HE-16GX6, manufactured by Koike Precision Instruments Co., Ltd.) was used to stir at 300 rpm, and the following evaluation was performed. Water solubility was evaluated based on standards. Table 1 shows the results.
• the dissolution time in the following evaluation criteria means the time from the start of the stirring to the disappearance of clumps of the cleaning composition by visual observation. (Evaluation criteria) ⁇ (excellent) ... dissolution time less than 5 minutes ⁇ (very good) ... dissolution time 5 minutes or more and less than 10 minutes ⁇ (good) ... dissolution time 10 minutes or more
• Examples 1 to 9 of the present invention had a high enzymatic activity residual rate and sustained enzymatic activity even after being stored at 40° C. for one month.
• Comparative Examples 1 and 2 with low glycerin and/or sorbitol content, Comparative Examples 3 and 4 with high water content, and Comparative Example 5 with low polyhydric alcohol content and high water content After storage at 40° C. for 1 month, the enzyme activity residual rate was remarkably decreased, confirming that the enzyme activity did not persist.
• the content of (C) is 25 to 60% by mass relative to the total amount of the cleaning composition
• the content of (D) is 5 to 30% by mass relative to the total amount of the cleaning composition
• glycerin as (C) and/or sorbitol and the content of glycerin and/or sorbitol is 10 to 60% by mass based on the total amount of the cleaning composition. It was confirmed that the decrease in activity can be suppressed. It was also confirmed that the cleaning composition of the present invention has excellent water solubility.
• the enzymatic activity (U/g) of the protease in the compositions of Examples 1-9 is all 1.5 (U/g).
• the enzyme activity (U/g) was obtained by adding 1 mL of (B) protease [Bioprase (registered trademark) XL-416F ( Nagase ChemteX Co., Ltd.)] was added and allowed to react at 30°C for 10 minutes. value.
• Formulation examples of the present invention are shown below. In addition, all formulation examples maintained the enzymatic activity and showed good enzymatic activity.
• ⁇ Face cleanser 1 face cleanser filled in a tube-shaped container> Water 30% by mass Glycerin 17% by mass 1,3-butylene glycol 8.85 mass% Proteolytic enzyme 0.1% by mass Lipolytic enzyme 0.1% by mass Dextrin 1.9% by mass Propylene glycol 0.15% by mass Lauric acid 5% by mass Myristic acid 10% by mass Palmitic acid 13% by mass Stearic acid 5% by mass Kaolin 1% by mass Potassium hydroxide 6% by mass Ascorbic acid 1% by mass Hydroxypropyl methylcellulose 0.5% by mass Perfume 0.3% by mass Polyquaternium-7 0.1% by mass Total amount 100% by mass pH (23°C) 9.2 Water solubility ⁇ * Proteolytic enzyme: Bioplase (registered trademark) XL-416F (manufactured by Nagase ChemteX Corporation) * Lipolytic enzyme: Relipase (registered trademark) A-10D (manufactured by Nagas
• ⁇ Face cleanser 2 (Face cleanser filled in a tube-shaped container)> Water 15% by mass Glycerin 30% by mass PEG-8 12.955% by mass Propylene glycol 0.075% by mass Protease 0.05% by mass Lipolytic enzyme 0.05% by mass Dextrin 0.95% by mass Lauric acid 5% by mass Myristic acid 9% by mass Palmitic acid 10% by mass Stearic acid 4% by mass Sodium pyrosulfite 0.02% by mass Potassium hydroxide 5% by mass Lauryl glucoside 6 mass% Hydroxypropyl methylcellulose 0.5% by mass Perfume 0.3% by mass Self-emulsifying glyceryl monostearate 1% by mass Polyquaternium-10 0.1% by mass Total amount 100% by mass pH (23°C) 9.2 Water solubility ⁇ * Proteolytic enzyme: Bioplase (registered trademark) XL-416F (manufactured by Nagase ChemteX Corporation) * Lipolytic enzyme
• ⁇ Facial cleanser 3 (face cleanser filled in a tube-shaped container)> Lauric acid 5% by mass Myristic acid 9% by mass Palmitic acid 10% by mass Stearic acid 4% by mass Proteolytic enzyme 0.12% by mass Glycerin 39% by mass Propylene glycol 0.18% by mass Purified water 24.4% by mass Potassium hydroxide 3.5% by mass Lauryl glucoside 2.5% by mass Self-emulsifying glyceryl stearate 1% by mass Serine 0.1% by mass 1,3-propanediol 1% by mass Loquat leaf extract 0.1% by mass Bilberry leaf extract 0.1% by mass Total amount 100% by mass pH (23°C) 8.0 Water solubility ⁇ * Proteolytic enzyme: Bioplase XL-416F (manufactured by Nagase Chemtech)
• ⁇ Facial cleanser 4 (face cleanser filled in a tube-shaped container)> Lauric acid 5% by mass Myristic acid 9% by mass Palmitic acid 10% by mass Stearic acid 4% by mass Proteolytic enzyme 0.12% by mass Glycerin 39.4% by mass Propylene glycol 0.18% by mass Purified water 21.2% by mass Potassium hydroxide 6.7% by mass Lauryl glucoside 2.5% by mass Self-emulsifying glyceryl stearate 1% by mass Trimethylglycine 0.1% by mass Trehalose 0.5% by mass Comfrey leaf extract 0.1% by mass Cha leaf extract 0.1% by mass Grapefruit extract 0.1% by mass Total amount 100% by mass pH (23°C) 11.0 Water solubility ⁇ * Proteolytic enzyme: Biopase XL-416F (manufactured by Nagase Chemtech)
• ⁇ Facial cleanser 5 (face cleanser filled in a tube-shaped container)> Lauric acid 8% by mass Myristic acid 8% by mass Palmitic acid 12% by mass Oleic acid 1.5% by mass Proteolytic enzyme 0.12% by mass Glycerin 33.5% by mass Propylene glycol 0.18% by mass Purified water 25% by mass Potassium hydroxide 6.5% by mass Sodium hydroxide 0.3% by mass Lauryl glucoside 2.5% by mass Self-emulsifying glyceryl stearate 1% by mass Serine 0.5% by mass Trehalose 0.5% by mass Loquat leaf extract 0.1% by mass Lemon extract 0.1% by mass Tea leaf extract 0.2% by mass Total amount 100% by mass pH (23°C) 9.5 Water solubility ⁇ * Proteolytic enzyme: Biopase XL-416F (manufactured by Nagase Chemtech)
• ⁇ Face cleanser 6 face cleanser filled in a tube-shaped container> Lauric acid 8% by mass Myristic acid 8% by mass Palmitic acid 12% by mass Isostearic acid 1.5% by mass Proteolytic enzyme 0.12% by mass Glycerin 34.289% by mass Propylene glycol 0.18% by mass Purified water 25% by mass Potassium hydroxide 6.5% by mass Sodium hydroxide 0.3% by mass Lauryl glucoside 2.5% by mass Self-emulsifying glyceryl stearate 1% by mass Trimethylglycine 0.5% by mass Seaweed extract 0.01% by mass Loquat leaf extract 0.001% by mass Grapefruit extract 0.1% by mass Total amount 100% by mass pH (23°C) 9.5 Water solubility ⁇ * Proteolytic enzyme: Biopase XL-416F (manufactured by Nagase Chemtech)
• the cleaning composition of the present invention is suitably used as a skin cleaning composition and a hair cleaning composition. Among others, it can be particularly preferably used as a facial cleansing composition (face wash).

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• 2022
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