WO2023013301A1 - プロテオグリカンを含む軟骨成分混合物の製造方法 - Google Patents

プロテオグリカンを含む軟骨成分混合物の製造方法 Download PDF

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Publication number
WO2023013301A1
WO2023013301A1 PCT/JP2022/025701 JP2022025701W WO2023013301A1 WO 2023013301 A1 WO2023013301 A1 WO 2023013301A1 JP 2022025701 W JP2022025701 W JP 2022025701W WO 2023013301 A1 WO2023013301 A1 WO 2023013301A1
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Prior art keywords
cartilage
proteoglycan
mass
production method
component mixture
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PCT/JP2022/025701
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English (en)
French (fr)
Japanese (ja)
Inventor
達治 高橋
俊昭 藤根
里沙 ワイズ
知也 岡本
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Ichimaru Pharcos Co Ltd
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Ichimaru Pharcos Co Ltd
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Publication date
Application filed by Ichimaru Pharcos Co Ltd filed Critical Ichimaru Pharcos Co Ltd
Priority to JP2022564511A priority Critical patent/JP7220941B1/ja
Priority to US18/577,697 priority patent/US20240317824A1/en
Priority to CN202280038478.6A priority patent/CN117460739A/zh
Priority to JP2022174079A priority patent/JP7846899B2/ja
Publication of WO2023013301A1 publication Critical patent/WO2023013301A1/ja
Anticipated expiration legal-status Critical
Priority to JP2026027340A priority patent/JP2026069717A/ja
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/461—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from fish
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals

Definitions

  • Proteoglycan (hereinafter sometimes referred to as "PG") is a glycoprotein in which several to several tens of glycosaminoglycans such as chondroitin sulfate and keratan sulfate are covalently bound to one core protein. It is widely distributed in the body such as skin and cartilage as one of the PG in cartilage forms aggregates with collagen and hyaluronic acid, and a typical cartilage-type PG is called aggrecan.
  • Aggrecan has a large amount of glycosaminoglycan sugar chains bound to its core protein, and has a binding region for hyaluronic acid and link protein on its N-terminal side.
  • the molecular weight of proteoglycan in the cartilage component mixture containing proteoglycan obtained by this second embodiment is preferably 400,000 to 650,000.
  • the residue was put into 550 L of 99% ethanol again and stirred at 50° C. for 30 minutes.
  • the residue was put into 550 L of 99% ethanol again and washed with stirring at 50° C. for 30 minutes.
  • the pulverized material was collected by centrifugation, dried under reduced pressure at a heating temperature of 35° C. or lower, and the obtained dried material was pulverized with a pulverizer to obtain about 40 kg of pulverized material.
  • - PG yield (%) Value (%) calculated by "((content of proteoglycan in obtained freeze-dried product)/(weight of nasal cartilage 400 (g))) x 100".
  • PG molecular weight (x 10 4 ) proteoglycan molecular weight.
  • the description of "72" in Example 10 of Table 2 indicates a molecular weight of 720,000 (720,000).
  • Collagen content (%) Collagen content in the freeze-dried product.
  • the cells were cultured for 24 hours in an environment of 37° C. and 5% CO 2 .
  • the medium was replaced with a sample-containing medium (200 ⁇ l of DMEM medium containing 0.1% FBS).
  • a control group was prepared in which the cells were cultured only in a DMEM medium containing 0.1% FBS without the prescribed sample.
  • the cells were cultured for 72 hours in an environment of 37° C. and 5% CO 2 .
  • the cell growth effect of the added samples (Sample 1 to Sample 3 groups) was evaluated using CellTiter-GloTM Luminescent Cell Viability Assay (Promega). Statistical significance of the obtained test results was evaluated using Dunnett's test.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
PCT/JP2022/025701 2021-08-06 2022-06-28 プロテオグリカンを含む軟骨成分混合物の製造方法 Ceased WO2023013301A1 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2022564511A JP7220941B1 (ja) 2021-08-06 2022-06-28 プロテオグリカンを含む軟骨成分混合物の製造方法
US18/577,697 US20240317824A1 (en) 2021-08-06 2022-06-28 Method for producing cartilage component mixture including proteoglycan
CN202280038478.6A CN117460739A (zh) 2021-08-06 2022-06-28 包含蛋白多糖的软骨成分混合物的制造方法
JP2022174079A JP7846899B2 (ja) 2021-08-06 2022-10-31 プロテオグリカンを含む軟骨成分混合物の製造方法
JP2026027340A JP2026069717A (ja) 2021-08-06 2026-02-24 プロテオグリカンを含む軟骨成分混合物の製造方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2021-129997 2021-08-06
JP2021129997 2021-08-06

Publications (1)

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WO2023013301A1 true WO2023013301A1 (ja) 2023-02-09

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JP (3) JP7220941B1 (https=)
CN (1) CN117460739A (https=)
WO (1) WO2023013301A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117919508A (zh) * 2024-01-25 2024-04-26 百瑞德（南京）生物科技有限公司 一种鼻软骨提取物的制备方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000095795A (ja) * 1998-09-18 2000-04-04 Seikagaku Kogyo Co Ltd プロテオグリカンのコアタンパク質のポリペプチド及びそれをコードするｄｎａ
JP2012236776A (ja) * 2011-05-09 2012-12-06 Institute Glycosmo Co Ltd プロテオグリカンの製造方法
JP2020127397A (ja) * 2019-02-08 2020-08-27 一丸ファルコス株式会社 プロテオグリカンの製造方法
JP2020200288A (ja) * 2019-06-12 2020-12-17 国立大学法人東京農工大学 変形性関節症予防又は改善剤

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3731150B2 (ja) 2000-08-22 2006-01-05 株式会社角弘 軟骨型プロテオグリカンの精製方法
JP6317053B1 (ja) 2018-01-17 2018-04-25 一丸ファルコス株式会社 プロテオグリカンの製造方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000095795A (ja) * 1998-09-18 2000-04-04 Seikagaku Kogyo Co Ltd プロテオグリカンのコアタンパク質のポリペプチド及びそれをコードするｄｎａ
JP2012236776A (ja) * 2011-05-09 2012-12-06 Institute Glycosmo Co Ltd プロテオグリカンの製造方法
JP2020127397A (ja) * 2019-02-08 2020-08-27 一丸ファルコス株式会社 プロテオグリカンの製造方法
JP2020200288A (ja) * 2019-06-12 2020-12-17 国立大学法人東京農工大学 変形性関節症予防又は改善剤

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117919508A (zh) * 2024-01-25 2024-04-26 百瑞德（南京）生物科技有限公司 一种鼻软骨提取物的制备方法

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Publication number Publication date
JP7220941B1 (ja) 2023-02-13
CN117460739A (zh) 2024-01-26
JP2026069717A (ja) 2026-04-23
JPWO2023013301A1 (https=) 2023-02-09
JP7846899B2 (ja) 2026-04-16
US20240317824A1 (en) 2024-09-26
JP2023024427A (ja) 2023-02-16

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