Classifications

• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
  • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
  • A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
  • A23L29/206—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
  • A23L29/212—Starch; Modified starch; Starch derivatives, e.g. esters or ethers
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
  • A23C20/00—Cheese substitutes
  • A23C20/02—Cheese substitutes containing neither milk components, nor caseinate, nor lactose, as sources of fats, proteins or carbohydrates
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
  • A23C20/00—Cheese substitutes
  • A23C20/02—Cheese substitutes containing neither milk components, nor caseinate, nor lactose, as sources of fats, proteins or carbohydrates
  • A23C20/025—Cheese substitutes containing neither milk components, nor caseinate, nor lactose, as sources of fats, proteins or carbohydrates mainly containing proteins from pulses or oilseeds
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
  • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
  • A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
  • A23L29/206—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
  • A23L29/238—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin from seeds, e.g. locust bean gum or guar gum

Definitions

• This application relates generally to plant-based soft cheese products, including plant-based cream cheese products.
• plant-based cheese products have been able to replicate certain attributes of dairy-based cream cheese products.
• plant-based cheese cream products often do not have the appearance, taste, or texture expected of dairy-based cream cheeses, including spread ability, indeed, some plant-based cream cheese products do not have a smooth, creamy texture and may be difficult to spread.
• currently available plant-based cream cheese products often have off-notes or aftertastes. These plant-based cream cheese products are not as well accepted by consumers who expect a cooking and eating experience that replicates dairy-based cheeses.
• FIG. l is a schematic diagram of a process tor making a plant-based cheese product according to some embodiments
• FIG. 2 is a black and white copy of an originally colored photograph of a substrate with a cream cheese type plant-based food product spread thereon;
• FIG. 3 is a black and white copy of an originally colored photograph of two exemplary cream cheese type plant-based food products;
• FIG. 4 is a black and white copy of an originally colored photograph of a substrate with example and comparative example cream cheese type plant-based food products spread thereon;
• FIG. 5 and FIG. 6 are light microscopy images, with a 100 mhi scale bar, of a comparative example cream cheese type plant-based rood product
• FIG. 7 is a light microscopy image, with a 100 pm scale bap of a comparative example cream cheese type plant-based food product
• FIG. 8 and FIG. 9 are light microscopy images, with a 100 pm scale bap of an example cream cheese type plant-based food product
• FIG. 10 and FIG. 11 are light microscopy images, with a 100 pin scale bap of an example cream cheese type plant-based food product
• FIG, 12 is a graph of fat droplet size distribution of example and comparative example cream cheese type plant-based food products Illustrating the frequency distribution percentage (Y axis) as a function of the diameter of the samples (am. X axis);
• FIG. 13 is a graph of fat droplet size distribution of example and comparative example cream cheese type plant-based food products illustrating the cumulative distribution percentage (Y axis) as a function of fire diameter of the samples (mth, X axis);
• FIG. 14 is a black and white copy of an originally colored macrograph of a substrate with example and comparative example cream cheese type plant-based food prod acts spread thereon;
• FIG. 15 is a graph of the light intensity of example and comparative example cream cheese type plant-based food products illustrating the light intensity of the samples (Y axis) as a function of line position (X axis);
• FIG. 16 is a graph of the mean intensity of example and comparative example cream cheese type plant-based food products illustrating the mean intensity of the samples (Y axis) as a function of area (X axis);
• FIG, 17 is a graph produced from rheometer temperature sweeps of example and comparative example cream cheese type plant-based food products illustrating firmness of the samples (Pa, Y axis) over temperature (°C, X axis);
• FIG. 18 is a graph produced from rheometer temperature sweeps of example and comparative example cream cheese type plant-based food products illustrating viscosity of the samples (Pa- s, Y axis) over temperature GC, X axis);
• FIG. 19 is a graph produced from rheometer temperature sweeps of example and comparative example cream cheese type plant-based food products illustrating Tan d of the samples (Y axis) over temperature (°C, X axis)
• FIG. 20 is a black and white copy of an originally colored photograph of of example and comparative example cream cheese type plant-based food products
• FIG. 21 is a scatter plot illustrating the a* (green-red) value (Y axis) and b* (blue- yellow) value (X axis) of example and comparative example cream cheese type plant-based food products;
• FIG. 22 is a bar graph illustrating the IX (Lightness) value of example and comparative example cream cheese type plant-based food products
• a plant-based cheese product which, m some approaches, may be in the form of a soft plant-based cheese product, such as a plant-based cream cheese product or plant-based cheese spread.
• plant-based refers to a product or ingredient that is free of animal-based proteins, such as dairy proteins, and comprises a plant- derived protein.
• the plant-based cheese products have an appearance, taste, and texture similar to a dairy-based cream cheese.
• the plant-based cheese products are in the form of a stable emulsion.
• the fat droplets are homogeneously dispersed in Lhe plant-based cheese products and no or minimal phase separation (syneresis) occurs for at least about four weeks, in another aspect at least about 8 weeks, and in another aspect at least about 12 weeks storage at refrigeration temperatures,
• Dairy-based cream cheeses are generally characterized by a soft, smooth texture and relatively high fat content (e.g., about 23-35 percent fat by weight of the finished product).
• the plant-based cream cheese products provided herein deliver the desired soft texture and spreadabiiity of a conventional dairy-based cream cheese product and, in some approaches, effective to do so at lower amounts of fat than in the conventional dairy-based cream cheese products.
• the plant-based cheese product disclosed herein may be free of animal-based proteins (including dairy-based proteins), animal-based proteins cannot be relied upon to produce the desired texture, including spreadabiiity at refrigeration temperatures of conventional cream cheeses.
• a plant-based cheese product with characteristics consistent with consumer expectations for a dairy-based cream cheese product could be obtained through the combination of a plant-based protein, a stabilizer, a starch-based thickening agent, and a tat component.
• the plant-based cheese product is further characterized by a desirable opaque appearance at both refrigerated and elevated temperature, such as a temperature at which the product is likely to be consumed (e.g., cream cheese on a toasted bagel).
• the plant-based cheese products described herein are uniquely able to maintain opacity at elevated temperatures (e.g., up to about 55°C).
• the plant-based cream cheese product includes a plant-based protein, a stabilizer, a starch-based thickening agent, and a fat component.
• a method of making the plant-based cheese product includes mixing water, a plant-based protein, a thickening agent, a stabilizer, and a fat component to form a mixture.
• the method may further include heating the mixture to a temperature within the range of about :150°F to about 200° F, such as via direct steam injection, to pasteurize the mixture; and homogen izing the heated mixture to form the plant-based cheese product in the form of a stable emulsion.
• the method may include heating the mixture to a temperature within the range of about 150°F to about 200°F via indirect steam in j ection to pasteurize the mixture; and homogenizing the heated mixture to form the plant-based cheese product.
• the heating such as by injecting steam (directly or indirectly), may occur before the homogenizing.
• the method may further include cooling the plant-based cheese product to rein geration temperature.
• a method of making the plant-based cheese product includes adding a plant-based protein to water to form a first mixture.
• the method may further include melting the fat component and adding the melted fat component, a stabilizer, and a thickening agent to the first mixture and mixing to form a second mixture.
• the second mixture is then heated to pasteurize the mixture, and then homogenizing the second mixture to form the plant- based cheese product in the form of a stable emulsion.
• the heating includes injecting steam directly into the second mixture to pasteurize the second mixture, and homogenizing the second mixture to form the plant-based cheese product.
• the method includes indirect steam heating of the second mixture to pasteurize the second mixture (e.g., via use of a thermally jacketed heating vessel), and homogenizing the second mixture to form the plant-based cream cheese product.
• the heating by steam may occur before homogenization.
• direct steam injection may be advantageous to avoid introduction of off colors and flavors to the product.
• the method of making the plant-based cheese products specifically do not include a fermentation step and the resulting plant-based cheese product may be characterized as a non-iermented plant-based cheese product.
• the terms "fermentation,” ' “fermented,” and the like refer to a process involving incubating a substrate, such as a carbohydrate, in the presence of a microorganism for a period of time in which the microorganism converts the substrate info an alcohol or and.
• a substrate such as a carbohydrate
• a microorganism for a period of time in which the microorganism converts the substrate info an alcohol or and.
• a starch or sugar is converted to lactic acid by yeast or bacterial strains.
• the present methods and plant-based cheese products do not include a lactic acid fermentation step.
• the method of making the plan t-based cheese products may include a fermentation step.
• lactic acid bacteria i.e., bacteria that produce lactic acid as a product of fermentation
• any Lactococcus lactis, Lactococcus crcrnoris, Streptococcus lactis, Streptococcus thermophilus , Lactobacillus helve ticus, and Lactobacillus bulgartcus may be used .
• Fermentation is generally carried out until a desired pH is achieved (e.g., between about 3.5 to about 5.0, in another aspect about 3.8 to about 4.8, and in another aspect about 4.0 to about 4,4),
• the plant-based cheese product described herein may be formed into any desirable shape.
• the plant-based cheese product is a cream cheese product that is formed into a soft block or filled into a container.
• Tire plant-based cheese product includes a plant-based protein. Any suitable plant- based protein may be used in the plant-based cheese product.
• the plant-based protein comprises one or more of faba bean protein (also known as fava bean protein), soy protein, lentil protein, potato protein, chickpea protein, canola protein, and pea protein. It has been found drat some plant-based proteins may impart off color or off flavor to the resulting plant-based cheese product. Therefore, in some approaches, the plant-based protein is selected based on the impact of the plant-based protein on the color and/or flavor of the final plant- based cheese product.
• soy protein, faba bean protein, and chickpea protein products may be particularly suitable for cream cheese applications.
• Faba bean, soy, or chickpea protein resulted in final products closer in color to conventional dairy- based cream cheese, while inclusion of pea or lentil protein resulted in cheese products with a more yellow or tan color, and inclusion of potato protein resulted in cheese products with a gray hue,
• Tire plant-based protein may be In the form of an isolate, a concentrate, or a flour, in some approaches, the plant-based protein is in the form of an isolate or a concentrate that contributes to emulsification of the plant-based cheese product. While not wishing to be limited by theory, it is presently believed that other non-protein componen ts of the protein isolate or concentrates may beneficially contribute to the texture of the cheese product, in some aspects, tire plant-based protein is the only source of protein in the plant-based cheese product, in this respect, the plant-based cheese product includes no animal- or dairy-based proteins, including, for example, casein and whey.
• the plant-based cheese product includes no nut-based proteins, including, for example, one or more of almond protein, peanut protein, and cashew protein. Additionally, or alternatively, the plant-based cheese product may be free of one or more of oat protein, rice protein, wheat protein, and/or sunflower seeds.
• the plant-based protein is present in an amount within tire range of about 0.2 wi% to about 8 wt% crude protein, in another aspect about 0,25 wt% to about 8 vvt% crude protein, in another aspect about 0,3 wt% to about 8 vvt% crude protein, in another aspect about 0,35 wt% to about 8 wt% crude protein, in another aspect about 0,2 wt% to about 6 wt% crude protein, in another aspect about 0.25 wt% to about 6 wt% crude protein, in another aspect about 0,3 wr% to about 6 wi% crude protein, in another aspect about 0.35 wt% to about 6 w ⁇ % crude protein, in another aspect about 0,2 wt% to about 5 wt% crude protein, in another aspect about 0.25 wi% to about 5 wi% crude protein, in another aspect about 0.3 wt% to about 5 wt% crude protein, in another aspect about 0.35 wt% to about 5 wt% crude protein, in another aspect about
• the amount of cmde protein in a plant-based protein ingredient may depend on die form of the protein -containing ingredien t (e.g., whether the ingredient is in the form of an isolate, a concentrate, or a flour).
• the amount of cmde protein is the amount of protein contributed by any protein-containing ingredien t.
• the commercially available VITESSENCETM Pulse 3600 (Ingredion) faba bean protein product includes about 60% protein and about 40% non-protein components. If a plant-based cheese product includes about 2 wt% VITESSENCETM Pulse 3600 faba bean protein product, the plant- based cheese product will include about 1.2 wt% crude protein, for purposes herein.
• the amount of crude protein in a plant-based protein ingredient; or in the plant-based cheese product may be measured by the Association of Official Analytical Chemists (AOAC) Official Method 992.15 (which is incorporated herein by reference in its entirety). Additionally, or alternatively, the amoun t of crude protein in a plant-based protein ingredien t or in the plant- based cheese product may be measured by the Dumas Method.
• AOAC Association of Official Analytical Chemists
• the plant-based protein may be the only emulsifier in the plant- based cheese product.
• the plant-based cheese product is free from lecithin, monoglycerides, diglycerides, polyethylene glycol propylene glycol alginate, and polysorbate.
• the plant-based cheese product may also be free of any one or more of giucono-Deita-Lacione, tricaicium phosphate, sugar, beta Carotene (color), and sodium citrate.
• a plant-based protein and in particularly in combination with a stabilizer and a starch-based thickener, has surprisingly been found to provide significant benefits to the appearance and performance of the plant-based cheese product.
• the stabilizer, starch-based thickener, and fat component interact with fire plant-based protein in the final food product to contribute to the opacity of the product.
• plant- based cheese products prepared without a plant-based protein may have a white appearance and opacity at refrigeration temperature but a thinner hot viscosity that results in a loss of opacity when the plant-based cheese product is applied on a hot substrate.
• a plant-based cheese products is prepared with a plant-based protein in combination with the stabilizer, starch-based thickener, and fat component as described herein, the product maintains its opacity when applied on a hot substrate, such as a slice of toasted bread or bagel. It is presently believed that the plant-based protein stabilizes the product matrix and maintains smaller droplets of the fat component.
• the plant-based protein and starch-based thickener molecules contribute to light scattering at elevated temperatures. Hie inclusion of a plant-based protein also allows for lower percent of light transmission at a wavelength of 865 nm.
• the stabilizer, starch-based thickener, and fat component interact with the plant-based protein in the final food product to contribute to the texture of the product.
• plant-based cheese products prepared without a plant-based protein may have a soft, smooth texture similar to a dairy-based cream cheese, but a thinner, less firm texture at temperatures above 25 U C.
• the stabilizer, starch-based thickener , and iat component as described herein, the product retains more firmness, emulsion stability, and opacity than a similar plant-based cheese product but that was prepared without a plant-based protein,
• the plant-based cheese product further includes a fat component having a solid fat content at 10°C of about 50% to about 90%, in another aspect about 55% to 90%, in another aspect about 55% to about 85%, and in another aspect about 60% to about 85%, and a solid fat content at 20°C of about 15% to about 45%. in another aspect about 20% to about 45%, in another aspect of about 20 to about 40%, and in another aspect about 25% to about 40%.
• Ore fat component has a solid fat content of about 50% to about 90% at 10°C and a solid fat content of about 15% to about 45% at 20°C, in another aspect a solid tat content of about 55% to about 90% at 10°C and a solid fat content of about 20% to about 45% at 20°C, in another aspect a solid fat content of about 55% to about 85% at 10°C and a solid tat conten t of about 20% to about 45% at 20°C, and yet another aspect a solid fat content of about 60% to about 85% at 10°C and a solid fat content of about 25% to about 40% at 20°C
• the fat component when the fat component has a solid fat content within the specified ranges, the fat component may behave functionally similarly to butterfat, which may contribute to ihe plant-based cheese product having a flavor profile, cold texture, and melt profile similar to a dairy-based cheese.
• Ihe solid fat content of ihe fat component may be measured by differential scanning calorimetry (DSC), In differential scanning calorimetry, a 10 mg sample in a hermetically sealed pan may be heated from -50CFC to lOOCFC at a heating rate of ICfC/ minute, and the heat flow rate may be measured as a function of temperature. From the heat flow vs. temperature curve, a solid fat content vs. temperature curve may be calculated.
• DSC differential scanning calorimetry
• any suitable fat component comprising one or more solid fats, liquid oils, or combination thereof having the specified solid fat content may be used.
• the tat component comprises one or more of vegetable- or plan t-based oils, such as coconut oil, palm oil, palm oil fraction, shea butter, and shea olein.
• the fat component further comprises one or more of soybean oil, sunflower oil, olive oil, canola oil, peanut oil, sesame oil, and corn oil to provide a blend of ingredients to provide the desired solid fat; content a! Lhe respective temperatures.
• the oil is a refined od (e.g., refined coconut oil).
• the fat component comprises a combination of coconut oil and sunflower oil, for example, the commercially available AKOVEGTM oil (sold by AAK USA Inc. ⁇ .
• the fat component comprises coconut oil.
• the plant-based cheese product may be free from palm oil and palm oil fraction.
• the fat component is present in an amount within the range of about 10 wt% to about 50 wt%, in another aspect about 10 wt% to about 45 wt%, in another aspect about 15 wt% to about 40 wt%, in another aspect about 15 wt% to about 35 wt%, in another aspect about 15 wt% to about 25 wt%, and in another aspect about 20 wt% to about 30 wt%, based on a total weight of the plant-based cheese product.
• the plant-based cheese product further includes a stabilizer.
• the stabilizer may be any suitable hydrocolloid or fiber.
• the stabilizer assists with water management and texture of the plant-based cheese product, in some aspects, the hydrocoi!oid includes one or more of muiin, pectin, carboxymethylceilulose, carrageenan, gum Arabic, xanthan gum, locust bean gum, mid guar gum.
• the hydrocolloid comprises a combination of xanthan gum, locust bean gum, and guar gum, such as the commercially available TIC Stabilizer 424 (!ngredion).
• the hydrocolloid comprises locust bean gum.
• the fiber is a vegetable fiber, and particularly insoluble fiber.
• suitable vegetable fibers include, for example, flax fiber, hemp fiber, and pile fiber.
• One exemplary flax fiber is Hi-SMOOTH® (flax fiber from HIFOOD, Parma, Italy).
• the stabilizer may act as an emulsion stabilizer in the plant-based cheese product. In some aspects, the stabilizer may also provide a thickening function.
• lhe stabilizer is present in an amount within the range of about 0.01 wt% to about 10 wt%, in another aspect about 0.01 wt% to about 5 wt%, in another aspect about 0,01 wt% to about 1 wi%, in another aspect about 0.05 wt% to about 1 wi%, in another aspect about 0.1 vvt% to about 5 wt %, in another aspect about 0.25 wt% to about 5 wt %, in another aspect about 0.1 wt% to about 3 wi%, in another aspect about 0.25 wt% to about 3 wt %, in another aspect about 0.1 wt% to about 2 wt%, in another aspect about 0.25 wt% to about 2 wt %, and in another aspect about 0.1 wt% to about 1 wt%, in another aspect about 0,25 wt% to about 1 wt % stabilizer, based ou a total weight of the plant-based cheese product.
• the plant-based cheese product further includes a thicken lug agent, such as a starch- based thickening agent.
• the thickening agent can contribute to desired texture of the plant- based cheese product.
• Suitable thickening agents include, for example, starches such as potato starch, com starch, tapioca starch, arrowroot starch, or rice starch.
• ihe starch is shear tolerant
• starch is able to withstand homogenization (e.g,, single stage homogenization at 165 bar in a GEA Twin Panda Dynamic Homogenizer) at a temperature of 82°C in order to contribute a measurable increase in viscosity to the final product upon cooling to 5°C
• measurable increase in viscosity means an at least 5% (or in some aspects at least 10%) increase in complex viscosity upon cooling as compared to an otherwise identical product made without a starch-based thickening agent and including additional water in place of the starch-based thickening agent.
• the starch is a modified starch, such as an enzymatically converted or acid- thinned starch, in some examples, the starch Is an enzymatically-converted potato starch, such as the commercially available ETENIATM 457 starch (Codperatie Avebe U.A.).
• the starch is or comprises a low dextrose equivalent (DE) maltodexirin, such as having a DE of 10 or less, in another aspect a DE of 5 or less, in another aspect a DE of 3 or less, and in another aspect a DE of 2,
• DE dextrose equivalent
• the starch is thermoreversible such that it is fiowabie at hot temperatures and sets as it cools, in this manner, a thermoreversible starch may provide a spreadable texture to a cooled plant-based cheese product.
• inclusion of a thickening agent may contribute to the textural characteristics of ihe plant-based cheese product, inclusion of a thickening agent may provide textural characteristics that replicate a dairy-based cheese product.
• the thickening agent may provide a texture that is firm enough to scoop, spread, and quickly dissipate in the mouth.
• the starch-based thickening agent is present m an amount within the range of about 1 wt% to about 25 wt%, based on a total weight of the plant-based cheese product.
• the thickening agent is present in an amount within the range of about 1 wt% to about 20 wt% , in another aspect about 1 wi% to about 15 wi%, in another aspect about 1 wt% to about 12 wt%, in another aspect about 3 wt% to about 10 wt%, in another aspect about 3 w% to about 8 wl%, based on a total weight of the plant-based cheese product.
• Tire plant-based cheese product further includes water, in some aspects, the plant- based cheese product includes water m an amount effective to provide a moisture of the plant-based cheese product within the range of about 50 wt% to about 80 wt%, in another aspect about 50 wt% to about 75 wi%, in another aspect abou t 55 wt% to about 75 wt%, in another aspect about 55 wt% to about 70 wt%, or in another aspect about 60 wt% to about 70 wt%, by weight of hie plant-based cheese product.
• the plant-based cheese product may further include an acidulant.
• the plant-based cheese product includes an acidulant in an amount effective to provide a pH in the plant-based cheese product of about 3.5 to about 5.0, in another aspect about 3,8 to about 4,8, and in another aspect about 4.0 to about 4.4.
• Any suitable acidulant may be used. Suitable acids include malic acid, citric acid, acetic add, phosphoric acid, and lactic acid.
• Ore acidulant comprises one or more of citric acid, sorbic add, and lactic acid.
• the inclusion of the acidulant to a provide a pH in the described ranges contributes to microbial stability of the product as well as providing desirable flavor.
• the acidulant may be separately added to the ingredients of the plant-based cheese product and/or the acidulant maybe generated via a fermentation step. For example, lactic add may be generated during fermentation with an acid- generating bacteria, such as lactic add bacteria.
• the plant-based cheese product may further include one or more additional ingredients, such as salt, a preservative (e.g., sorbic acid), a colorant, and flavors.
• additional ingredients such as salt, a preservative (e.g., sorbic acid), a colorant, and flavors.
• any suitable natural or artificial flavors may be used, such as one or more of garlic, herbs (e.g., chives, parsley, basil), spices (e.g., cinnamon), fruit (e.g., strawberry, blueberry, pineapple, peach, and the like), nuts (e.g., pecan), pepper (e.g,, jaiapeno, chipotle, bell pepper), sweetener (e.g., honey, brown sugar, sucrose), olive, bacon, salmon, and vegetable (e.g., onion), in some aspects, fire one or more flavors include a rnasking-iype flavor to mask fire taste of another ingredient in the plant-based food product. In another aspect, one or more flavors may be included to build back positive dairy notes to replicate the taste of a dairy-based cheese product.
• herbs e.g., chives, parsley, basil
• spices e.g., cinnamon
• fruit e.g., strawberry, blueberry, pineapple, peach, and the like
• nuts e.g.,
• the plant-based cheese product may be free from one or more of nut-based proteins, almond protein, peanut protein, cashew protein, oat protein, rice protein, wheat protein, sunflower seeds, non -plant-based protein emulsifiers, lecithin, monoglycerides. diglycerides, polyethylene glycol, propylene glycol alginate, po!ysorbaie, palm oil, and palm oil fraction.
• the plant-based cheese products described herein can be made by a variety of methods.
• the plant-based cheese products can be made by a method comprising combining a plant-based protein, a fat component, a starch-based thickening agent, water, a stabilizer, and an acidulani to form a mixture.
• the fat component may be melted, such as m a cooker, before it is added to the mixture.
• Other optional ingredients such as flavors or salt may be added at this point or later in the process,
• I he ingredien ts are mixed in a mixer.
• the ingredients may be mixed in a mixer having direct steam injection capabilities by which steam may be injected directly into the product mixture, in tills manner, the ingredients may be heated via direct steam injection while mixing.
• direct steam injection steam is introduced directly into or above the product mixture in the mixing vessel.
• direct steam injection steam may condense into the product mixture and contribute to moisture levels in the product mixture
• lu indirect steam injection steam is separate from the product mixture and hears the product mixhire indirectly by contacting a surface in thermal communication with the product mixture, such as a steam jacketed mixing vessel.
• the mixture of ingredien ts is heated for a time and to a temperatu re effective to pasteurize the mixture, such as to a temperature within the range of about 150“F to about 200°F, about 160°F to about 200°F, about IblFF to about 190°F, or in some aspects about 170°F to about 19(FF, for a time period of, for example, about 1 second to about 5 minutes.
• a temperatu re effective to pasteurize the mixture such as to a temperature within the range of about 150“F to about 200°F, about 160°F to about 200°F, about IblFF to about 190°F, or in some aspects about 170°F to about 19(FF, for a time period of, for example, about 1 second to about 5 minutes.
• High- temperature-short-time pasteurization methods may also be used, if desired.
• the time of the heat treatment may depend m part on the temperature of the heat treatment
• the starch-based thickening agent may gelatinize before the mixture is heated to such temperatures and hearing may primarily act to pasteurize the mixture.
• heating may both pasteurize the mixture and gelatinize the starch-based thickening agent.
• the pasteurization treatment is earned out while continuing to mix the product.
• heating the mixture via direct steam injection as opposed to indirect steam injection may improve the appearance of the final plant-based cheese product.
• healing the mixture via direct steam injection may provide a color and shine of the final plant-based cheese product that more closely imitates the color and shine of a dairy-based cheese product;.
• healing the mixture via direct: steam injection may provide an off-white color and reduce browning as compared to indirect steam heating (e.g,, when a fermentation step is used).
• heating the mixture via direct steam injection may provide a shiny, wet appearance which may be desirable for the final plant- based cheese product.
• the ingredients may be mixed in a mixer having indirect steam injection capabilities.
• the ingredients may be heated via indirect steam injection while mixing.
• the mixture of ingredients is heated for a time and to a temperature effective to pasteurize the mixture and/or gelatinize the starch-based thickening agent (as described above). It is contemplated drat; in these aspects, a plant-based cheese product: having an appearance replicating that of a dairy-based cheese product (e.g., a desirable opaque appearance at elevated temperature) may be achieved.
• the mixture is then homogenized or treated with high shear to provide the plant- based cheese product.
• the term "homogenize” is used to encompass both homogenization and high shear treatments capable of providing a homogenous mixture.
• the mixture may he homogenized using any suitable equipment to provide a smooth texture to the plant-based cheese product, for example, via a homogenizer or a shear pump.
• homogenization provides a homogenous mixture and may distribute ingredients, such as the stabilizer, evenly throughout the product. It is contemplated that homogenization may provide a smooth texture in die plant-based cheese product.
• die plant-based cheese product may be characterized as an emulsion
• the mixture is homogenized at an elevated pressure, that is, a pressure greater than atmospheric pressure.
• the mixture is homogenized at pressure within the range of about 100 psi to about 3000 psi, about 100 psi to about 2000 psi, about 500 psi to about 3000 psi, about 500 psi to about 1500 psi, about 700 psi to about 1300 psi, about 800 psi to about 2500 psi, or about 800 psi to about 1200 psi.
• the pressure selected may depend in part on the particular equ ipment used. Any suitable pressure that provides a desired smooth texture to the plant-based cheese product may be used.
• the plant-based cheese product has a fat droplet size distribution that enables the plant-based cheese product to have, at elevated temperatures, an opaque appearance and/or a soft, smooth texture similar to a dairy-based cream cheese.
• the tat droplet size distribution map be measured using a Bruker time-domain nuclear magnetic resonance droplet size analyzer (Bruker TD-NMK droplet size analyzer).
• a decay curve (intensity vs. time) of the NMR field may be used to derive the fat droplet size distributions.
• the mixture may be homogenized to achieve a D50 (i.e., 50% of the fat droplets diameters are below ibis value) at 40°C of 7 pm or less, iu another aspect 6.75 pm or less, in another aspect 6.5 pm or less, in another aspect 6.25 pin or less, in another aspect 6.0 am or less.
• a D50 i.e., 50% of the fat droplets diameters are below ibis value
• the mixture may be homogenized to achieve a D50 at 40°C within the range of about 1.5 pm to about 7 pm, in another aspect within the range of about 1.5 pm to about 6.75 pm, in another aspect within the range of about 1.5 pm to about 6.5 pm, in another aspect within the range of abou t: 1.5 pm to about 6.25 urn, in another aspect within the range of about 1.5 pm to abou t 6.0 urn
• the plant-based cheese product may nave width of distribu tion (i.e,, the standard deviation y--— ⁇ - 4 ⁇ - ⁇ — m, wherein 97,5% of the fat: droplets diameters are below the D97.5 and 2,5% of the fat droplets diameters are below the D2.5 value) at 40°C of 5,0 pm or less, in another aspect 4.5 pm or less, in another aspect 4.0 pm or less, and in another aspect; 3.5 pm or less. It is presently believed that the D50 values in combination with the width of distribution of the oil droplet size is most indicative of the emulsion stability of the product.
• Ad d it ion ally or alternatively, the mixture may be homogenized to achieve a D97.5
• the mixture may be homogenized to achieve a D2.5 (i.e., 2.5% of the fat droplets diameters are below this value) at 40°C of 3.0 pm or less, 2,75 pm or less, 2,5 pm or less, 2.25 pm or less, or 2,0 pm or less.
• the plant-based cheese product has a complex viscosity at a frequency of 10 rad/s and a temperature of 25°C and 37°C that enables the plant-based cheese product to have a soft, smooth texture similar to a dairy-based cream cheese at those temperatures.
• the temperatures of 25°C and 37°C are particularly informative for product performance for a cream cheese type product as 37°C represents a temperature of a hot bagel upon winch the product may be spread and 25°C represents a hkeiy temperature of the product when the product is consumed,
• Tire complex viscosity indicates the stability of the emulsion at both 25°C and 37°C.
• the plant-based cheese product has a complex viscosity at a frequency of 10 rad/s and a temperature of 25°C within the range of about 400 Pa ' s to about 1200 Pa ⁇ s, the range of about 400 Pa ⁇ s to about 1150 Pa ⁇ s, the range of about 400 Pa ⁇ s to about 1000 Pa ⁇ s, the range of about 400 Pa ⁇ s to about 900 Pa - s, the range of about 400 Fa - s to about 800 Pa ⁇ s, the range of about 400 Pa ⁇ s to about 750 Pa ⁇ s, the range of about 400 Pa ⁇ s to about 700 Pa ⁇ s, the range of about 400 Pa - s to about 600 Pa ⁇ s.
• the plant-based cheese product may have a complex viscosity at a frequency oi 10 rad/s and a temperature of 37°C within the range of about 300 Pa ⁇ s to about 1000 Pa ⁇ s, the range of about 300 Pa ⁇ s to about 750 Pa ⁇ s, the range of about 300 Pa - s to about 600 Pa ⁇ s, the range of about 300 Fa ⁇ s to about 500 Pa ⁇ s, the range of about 300 Pa ⁇ s to about 400 Fa ⁇ s, the range of about 320 Pa ⁇ s to about 1000 Pa ⁇ s, the range of about 340 Pa ⁇ s to about 1000 Fa - s, the range of about 350 Pa ⁇ s to about 1000 Fa - s, the range of abou t 375 Pa - s to about 1000 Pa ⁇ s.
• the plant-based cheese product has an elastic modulus at a temperature between 25°C and 37°C that enables the plant-based cheese product to have a soft, smooth texture similar to a dairy-based cream cheese at the corresponding temperature.
• Elastic modulus indicates the relative firmness of the products.
• the plant-based cheese product lias an elastic modulus at a temperature of 25°C within the range of about 4000 Fa to about 8000 Pa, the range of about 4000 Pa to about 7500 Pa, the range of about 4000 Pa to about 7000 Pa, the range of about 4000 Pa to about 6500 Fa, the range of about 4000 Pa to about 6000 Pa, the range of about 4000 Pa to about 5750 Fa, the range of about 4250 Fa to about 8000 Pa, ibe range of about 4250 Fa to about 7500 Pa, the range of about 4250 Pa to about 7000 Pa, Ore range of about 4250 Pa to about 6500 Pa, ibe range of about 4250 Pa to about 6000 Pa, the range of about 4250 Pa fo about 5750 Pa, the range of about 4500 Pa to about 8000 Pa, the range of about 4500 Pa to about 7500 Pa, the range of about 4500 Fa to about 7000 Pa, the range of about 4500 Pa to about 6500 Pa, the range of about 4500 Pa to about 6000 Fa, the
• the plant-based cheese product may have an elastic modulus at a temperature of 37°C within the range of about 3000 Pa to about 7000 Pa, the range of about 3000 Pa to about 6000 Pa, the range of about 3000 Pa to about 5500 Pa, the range of about 3000 Pa to about 5000 Pa, the range ot about 3000 Pa to about 4500 Pa, the range of about 3000 Pa to about 4000 Pa, the range of abou t 3150 Fa to about 7000 Pa, the range of about 3150 Pa to about 6000 Pa, the range of about 3150 Pa to about 5500 Fa, Ibe range of about 3150 Pa to about 5000 Pa, the range of about 3150 Fa to about 4500 Pa, the range of about 3150 Fa to about 4000 Pa, the range of about 3250 Pa to about 7000 Pa, the range of about 3250 Fa to about 6000 Pa, the range of about 3250 Pa to about 5500 Fa, the range of about 3250 Pa to about 5000 Pa.
• the range of about 3250 Pa to about 4500 Pa the range of about 3250 Fa to about 4000 Fa, the range of about 3400 Pa to about 7000 Pa, the range of about 3400 Fa to about 6000 Fa, the range of about 3400 Pa to about 5500 Fa, the range of about 3400 Pa to about 5000 Fa, the range of about 3400 Fa to about 4500 Fa, the range of about 3400 Fa to about 4000 Pa, the range of about 3500 Pa to about 7000 Fa, the range of about 3500 Fa to about 6000 Fa, the range of about 3500 Pa to about 5500 Pa, the range of about 3500 Pa to about 5000 Pa, the range of about 3500 Pa to about 4500 Pa, tire range of about 3500 Pa to about 4000 Pa, the range of about 3600 Pa to about 7000 Pa, tire range of about 3750 Fa to about 71300 Pa, the range of about 3250 Fa to about 5000 Pa, tire range of about 3500 Pa to about 4500 Fa, or the range of about 3600 Pa to about 4000 Fa.
• 100601 Complex viscosity and/or tire elastic modulus may be measured using rheological thermal analysis.
• a TA Instrument ARES-G2 Rheometer may he used to apply a sinusoidal shear strain to a 2 mm thick, 25 mm diameter disc of a sample while healing the sample at a rate of 5°C/minute from 0°C to 80°C, and the resulting stress wave may be measured.
• the test geometry may be a 25 mm cross hatched parallel plate with a 500 mm cross hatched bottom pettier plate.
• the geometry gap may be equal to the sample thickness (c.g . 2 mm).
• Tire samples may be loaded at 30 C C
• the axial force may be 10 g ⁇ 5 g, and the sampling rate may be 12 s/point.
• the complex viscosity and/or the elastic modulus as a function of temperature may be calculated from the stress-strain curve.
• the method of a making a plant-based cheese product includes adding a plant-based protein to water to form a first mixture, in some aspects, tire first mixture may be mixed for an amount of time suitable to allow the plant-based protein to hydrate.
• the method also includes melting a fat component having a solid fat content in the range of about 50% to about 80% at 10°C and about 15% to about 40% at 20°C
• Tire method further includes adding tire melted fat component, a stabilizer, and a thickening agent to the first mixture and mixing to form a second mixture.
• Other optional ingredients such as flavors or salt may be added to the second mixture at this point or later in the process.
• the ingredients are mixed in a mixer and, in some aspects, a mixer having direct steam injection capabilities.
• tire second mixture is heated to a temperature within the range of about Id ⁇ /T to about 200°F, about I60°F to about 200T, about 16CPF to about I90°F, or m some aspects about I7CFF to about 190*7.
• the thickening agent may gelatinize before the second mixture is heated to such temperatures and heating may primarily be used to pasteurize the second mixture. In other aspects, heating may both pasteurize the second mixture and gelatinize the thickening agent.
• the mixture of ingredients is heated to and held at a temperature effective to pasteurize the second mixture.
• the second mixture is heated via direct steam in j ection.
• the second mixture is heated via indirect steam in j ection (e.g., in a thermally jacketed vessel).
• the second mixture is also homogenized to provide the plant-based cheese product in the form ot a stable emulsion.
• the second mixture may be homogenized in using any suitable equipment capable of applying high shear to the mixture, for example, via a homogenizer or a shear pump. In some approaches, the second mixture is homogenized at an elevated pressure.
• the second mixture is homogenized to provide a homogenous mixture and lo evenly distribute the ingredien ts.
• the heating by injecting steam may occur before homogenization.
• any of the methods described herein may further comprise add ing water to any of the mixtures described above, including either the first mixture or the second mixture.
• Water may be added to the mixture before or after (direct or indirect steam) heating, in one approach, water is added to provide a moisture % of the plant-based food product within the range ot about 50% to about 80%, about 55% to about 75%, or about 60% to about 70%,
• any ot the methods described herein may further comprise add ing an acidulant to any of the mixtures described above, including either the first mixture or the second mixture.
• the acidulant may be added to the mixture before or after (direct or indirect) steam heating.
• the acidulant is added to provide a pH in the plant-based food product of about 3.5 to about 5.0, in another aspect about 3,8 to abou t 4.8, and in another aspect about 4.0 to about 4.4.
• any of the methods described herein may further comprise adding one or more of salt, a preservative, a flavor, and a colorant.
• Tire plant-based cheese products made by the methods described herein may be packaged into suitable consumer size containers.
• the plant-based cheese product is packaged into containers by "hot packing" procedures in which containers are filled at elevated temperatures (i.e., shortly after the pasteurization treatment and before the product has cooled to refrigeration temperatures).
• the plant-based cheese product is packaged into containers at a temperature within the range of about 145°E to about 195°F, about 155°F to about 195°F, about 155°F to about 185°E. or in some aspects about 165°F to about 185°F.
• the plant-based cheese product is packaged into containers by "cold packing” procedures in which containers are filled while the product is cold (i.e., at refrigeration temperatures).
• the plant-based cream cheese product includes faba bean protein (VITESSENCETM Pulse 3600 protein) as the plant-based protein, potato starch ⁇ ETENIATM 457 starch) as the thickening agent, and a blend of coconut and sunflower oils (AKOVEGTM oil) as [he fat component,
• Tire plant-based cream cheese product may be prepared by adding water to a preheated mixer with steam injection capability (Breddo), Faba bean protein is first added to the water and mixed to allow the protein to hydrate.
• the coconut oil and sunflower oil blend is then melted.
• the coconut oil and sunflower oil blend has a solid fat content in the range of about 61% to about 67% at 10°C, and about 25% to about 29% at 2(6(7 fire melted coconut oil and sunflower oil blend, citric acid, sail;, a blend of xanthan gum, locust bean gum, and guar gum, potato starch, flavor, sorbic acid, and lactic acid are then added to the mixture of water and protein.
• the mixture is then heated to 180°F via steam injection and recirculation.
• the temperature of the mixture reaches 17CFF.
• additional flavors may be added.
• the pci and moisture% of the mixture are tested. Lactic and is added as needed in an amount effective to provide a pH within the range of about 4,0 to about 4.4 ha the final plant-based cheese product. Water is also added as needed to adjust the moisture% within the range of about 60% to about 70% in the final plant-based cheese product.
• the mixture is then healed to 180°F and held at I80°F for 1 minute for pasteurization. Then, the mixture is added to a homogenizer and mixed at 1000 psi for an amount of time sufficient to produce a homogenous mixture with a smooth texture. I hi ⁇ healed mixture is then packaged into tubs and allowed to cool and is refrigerated.
• the final plant-based cream cheese product has an opaque, white color.
• the first plant-based cream cheese product was prepared using die formulation shown in Table 1 of Example 1.
• a comparative plant-based cream cheese product was prepared using the formulation shown in Table 2 below.
• the fust plant-based cream cheese product vvas prepared with faba bean protein, and the comparative plant-based cheese product was prepared without protein and, instead, the protein was replaced with SHiJR-Fil.® starch (Tale & Lyle).
• Each plant-based cream cheese product had a soft, spreadable texture and was spread onto a freshly toasted bagel.
• FIG, 2 provides images comparing the first plant-based cream cheese product to the comparative plant-based cream cheese product on the bagel to illustrate the effect of protein on product appearance when spread on a substrate at elevated temperatures.
• Example 3 j 0087] Two exemplary plant-based cheese products (Samples “A” and “B") were prepared using the formulation shown in Table 3 below.
• the mixture used to prepare the plant-based cream cheese product "A” was heated via direct steam injection.
• the mixture used to prepare the plant-based cream cheese product "B” was heated via indirect steam using a jacketed mixer.
• Sample “A” exhibited an off- white color and a shun, wet appearance while Sample “B” exhibited some browning and a duller appearance than Sample "A", FIG.
• Each of the example plant-based cheese products included faba bean protein (VITESSENCETM Pulse 3600 protein) as the plant-based protein.
• each of the samples i.e., the example plant-based cheese products and comparative example plant-based cheese products
• FIG. 4 provides images comparing the samples on the bagel to illustrate the effect of protein on product appearance when spread on a substrate at elevated temperatures.
• LM images Light microscopy (LM) images oi each of the samples were taken .
• the EM images were taken with a Zeiss lmager.M2 light microscope equipped with an AxoCam MRc digital camera and operated by Zen 2,6 Blue software.
• LM images of the Comp. Ex. A sample are shown in FIG. 5 and FIG. 6.
• FIG. 5 shows the Comp. Ex. A sample under differential interference contrast (DIC) optics of tire light microscope.
• FIG. 6 shows the Comp. Ex. A sample stained with Lugo! Iodine solution, a dye which stained the starch dark blue.
• DIC differential interference contrast
• FIG. 7 shows Ere Comp. Ex, B sample stained with Lugoi iodine solution, which stained the starch dark blue.
• FIG. 8 shows the Ex. 0.5 wt% Faba sample stained with Lugo! Iodine solution, which stained the starch dark blue.
• FIG. 9 shows the Ex. 0.5 wt% Faba sample stained wi!h Acid Fuchsin, a dye which stained the protein a pink color.
• FIG. 10 shows the Ex. 1 wt% Faba sample stained with Lugoi Iodine solution, which stained the starch dark blue.
• FIG. 11 shows the Ex, 1 wt% Faba sample stained with Acid Fuchsin. which stained the protein a pink color.
• the fat component in the Comp. Ex. A sample was present as free oil separating from the starch particles, rather than forming individual oil droplets in a stable emulsion.
• FIG. 7. in the Comp. Ex. B sample, some of the fat component was present as free oil separating from the starch particles and some of the fat component was present as droplets.
• the starch acts as a thickener, it did not significantly contribute to emulsion stability.
• the larger fat droplets were able to coalesce to form the pockets of free oil in the Comp. Ex. A sample and the Comp. Ex, B sample.
• each of the Ex. 1 wi% Faba sample and the Comp. Ex. A sample was spread onto a black substrate at room temperature (21 °C), The Ex. 1 wt% Faba sample was opaque while the Comp, Ex. A sample was translucent The images illustrate the effect of protein on product appearance at room temperature.
• the Comp. Ex. A sample had more variation m light intensity than the Ex. 1 wr% Faba sample.
• the Comp, Ex, A sample had high reflectance at certain line positions, indicating shininess, and low reflectance (i.e.. light passed through easily) at certain positions, indicating transiucence. in contrast, the Ex. 1 wt% Faba sample had a more even reflectance across positions, indicating opacity.
• the Comp, Ex. A sample had a higher mean intensity than the Ex. 1 wt% Faba sample, indicating that the Comp. Ex. A sample was brighter and less opaque than the Ex. 1 wt% Faba sample,
• the scattered photon count rates (kcps) of each of the samples vvas measured at a temperature of 25°Q 40 ': 'C and 60°C To measure the scattered photon count rale, a Malvern Instruments Zeta Sizer Ultra dynamic light scattering instrument was used. A laser laeam with a wavelength of 630 nm and a known photon count rate was sent through 2 mL of the sample in a cuvette, and the scattered light intensity vvas measured with the detector held at a 173-degree angle to the incident light beam. The intensity vs time curve was integrated for 2 minutes to get the average intensity of the scattered light. Tire derived mean count rates (kcps) of each of die samples is shown in Table 8.
• the extent of scattering is proportional to the number of particles and die size of particles in the sample.
• die addition of protein decreased the size of the fat component droplets, which decreased the scattering by the fat component droplets.
• the addition of protein also increased scattering due to the presence of die larger protein molecules.
• die Ex, 0.5 wi% Faba sample and the Fx, 1 wt% Faba sample had derived mean count rates (kcps) at 25°C and at 40°C that were similar to the respective derived mean count ra tes of die Comp, Ex, A sample and the Comp. Ex. B sample.
• the complex viscosity at a frequency of 10 rad/s (Pa - s) as a function of temperature f ' C) of each of the samples is shown in FIG, 18, and the Tan o as a function of temperature (°C) of each of the samples is shown in FIG. 19.
• the elastic modulus (Pa), loss modulus (Pa), Tan 0, and complex viscosity (Pa ⁇ s, at a frequency of 10 rad/s) of each of the samples at a temperature of 5°C (refrigerated temperature), 25°C (room temperature), 37°C (mouth temperature), and 80°C (processing temperature) are shown in Table 9. [0130] TABLE 9
• the samples are viscoelastic gels with elastic modulus values much greater than loss modulus values, the elastic modulus is approximately the same as firmness.
• the Ex. 0,5 wt% Faba sample and the Ex. 1 w ⁇ % Faba sample had a higher elastic modulus, and thus were firmer, than the Comp. Ex. A sample and the Comp. Ex. B sample at temperatures within the range of 25 U C to 55°C.
• the Ex, 0,5 wt% Faba sample end the Ex. 1 wt%
• Faba sample had a higher complex viscosity than the Comp. Ex. A sample and the Comp, Ex, B sample at temperatures within the range of 25°C to 55°C,
• FIG. 17, FIG. 18, and Table 9 indicate that the Ex, 0.5 wt% Eaba sample and the Ex. 1 wt% Eaba sample had a desirable dairy- like, spreadable texture sample at temperatures within the range of 25 A to 55°C.
• a simplified model cream cheese system was prepared to compare the effect of protein on resulting cream cheese products.
• the cream cheese products did not include starch- based thickening agents.
• Thirteen examples of plant-based cream cheese product were prepared. To prepare each of the examples, an initial mixture of protein, glucose, fat component and water was prepared. Then, a lactic acid culture, salt, and stabilizer were added, and the samples were fermented at 40°C for about 18 hours in order to reach a pH of less than 4,6. The cultures were commercially available cultures obtained from CHR Hansen. After fermentation, each sample was pasteurized in a water hath while mixed by hand. [0138] Tire general formulation of the initial and final mixtures for each of the examples is shown m Table II, The general formulation of the examples is shown in Table 11, with the wt% of each ingredient that was used (based on the total weight of the initial mixture).
• Culture dosing was 0.02 wt% for the starter culture and 0.01 wt % for each adjunct flavor culture.
• Each of the examples included coconut oil as the fat component and locust bean gam as the stabilizer.
• the plant-based protein and culture included in each of the examples are shown in Table 12.
• Ex. 13 was treated with citric acid to a fermentation start pH of 6,0,
• FIG. 20 provides images comparing each of the examples and European Union (EU) Philadelphia® cream cheese (referred to herein as "Phil EU"), hi FIG. 20, the examples are identified by the plant-based protein they contain.
• EU European Union
• Phil EU Philadelphia® cream cheese
• Ex. 1, Ex, 2, Ex. 5, Ex. 6, Ex. A Ex. 8, Ex. 12, and Ex. 13 had a desirable off- white color
• Tire a* (green-red) value and b* (blue-yellow) value of each of tire samples is shown in FIG, 21.
• the box in FIG. 21 indicates desirable a* value and b* value combinations.
• the L* (Lightness) value of each of the samples is shown in FIG. 22, IC values above the horizontal line in FIG. 22 desirable are desirable.
• Ex. 1, Ex. 2, Ex, 5, Ex. 6, Ex. 7, Ex, 8, Ex. 12, and Ex, 13 had desirable a*, b*, and L* values.
• Ex 8 had a L* value closest to the L* value of Phil USA.
• FIG. 20 through FIG, 22 indicate that soy, chickpea, and faba bean protein produce plant-based cream cheese products with desirable color.
• a plant-based cheese product comprising: a plant-based protein; a stabilizer; a thickening agent; and a tat component having a solid far content within the range of about 50% to about 80% at 10°C and about 15% to about 40% at 2(FC
• the presen t disclosure pertains to the plant-based cheese product of the first aspect, further comprising an acidulerit in an amount effective to provide a pH in the plant-based cheese product of about 3.5 to about 5.0.
• the present disclosure pertains to the plant-based cheese product of the first aspect or the second aspect, further comprising water in an amount effective to provide a moisture % of the plant-based cheese product of about 50% to about 80%.
• the present disclosure pertains to the plant-based cheese product of any one of the first aspect to the third aspect, wherein the plant-based protein comprises one or more of faba bean protein, pea protein, and soy protein .
• the present disclosure pertains io the plant-based cheese product of any one of the first aspect to the fourth aspect, wherein the fat component comprises coconut oil and sunflower oil.
• the present disclosure pertains to the plant-based cheese product of any one of the first aspect to the fifth aspect, wherein the thickening agent comprises a starch.
• the present disclosure pertains to the plan t-based cheese product of the sixth aspect, wherein the starch is an enzymatically converted potato starch. [0008] in an eighth aspect, the present disclosure pertains to the plant-based cheese product of any one of tire first aspect to the seventh aspect ,, wherein tire stabilizer comprises at least one hydrocolloid,
• the present disclosure pertains to the plant-based cheese product of the eighth aspect, wherein the at least one hydrocolloid comprises one or more of Irvulin, pectin, carboxymethylcelhhose, carrageenan, gum arable, xanihan gum, locust bean gum, and guar gum,
• the present disclosure pertains to the plant-based cheese product of the eighth aspect, wherein the at least oue hydrocolloid comprises a combination of xanihan gum, locust bean gum, and guar gum.
• the present disclosure pertains to the plant-based cheese product of the eighth aspect, wherein the at least one hydrocoi!oid comprises locust bean gum,
• the present disclosure pertains to the plant-based cheese product of any one of the first aspect to the eleventh aspect, wherein the plant-based cheese product is in the form of a cream cheese product.
• the present disclosure pertains to the plant-based cheese product of any one of the first aspect to the twelfth aspect wherein the plant-based cheese includes no animal-derived proteins.
• the present disclosure pertains to the plant-based cheese product of any oue of the first aspect to the thirteenth aspect, wherein the plant-based protein is present in an amount within the range of about 0.01 wt% to about 15 wt% crude protein, based on a total weight of the plant-based cheese product.
• the present: disclosure pertains to the plant-based cheese product of any one of the first aspect: to the fourteenth aspect, wherein the stabilizer is present in an amount within the range of about 0.01 wt% to about 5 wt%, based on a total weight of the plant-based cheese product; and the thickening agent is present in an amount within the range of about 1 wt% to about 25 wl%, based on a total weight of the plant-based cheese product,
• the present disclosure pertains to the plant-based cheese product of any one of the first aspect to tire fifteenth aspect, wherein the fat component is presenl in an amount within the range of about 15 wt% to about 35 wt%, based on a total weight of the plant-based cheese product.
• the present disclosure pertains to a method of making a plant-based cheese product comprising: mixing water, a plant-based protein, a thickening agent, a stabilizer, and a fat component to form a mixture, the fat component having a solid fat content within the range of about of about 50% to abou t 80% at 10°C and about 15% to abou t 40% at 20°C; heating the mixture to a temperature within the range of about 150° F to about 200°F via direct steam injection; and homogenizing the heated mixture to form the plant-based cheese product, wherein the heating by injecting steam may occur before or during the homogenizing.
• the present disclosure pertains to the method of the seventeenth aspect, further comprising filling the plant-based cheese product into a container.
• the present disclosure pertains to the method of the seventeenth aspect or the eighteenth aspect, wherein the mixture is heated via direct steam injection to a temperature within the range of about 150°F to about 20CPF for about 1 second to about 5 minutes.
• the present disclosure pertains to the method of any one of the seventeenth aspect to the nineteenth aspect, further comprising adding an acidulent to the mixture to provide a pH within the range of about 3.5 to 5.0 in the plant-based cheese product.
• the present disclosure pertains to the method of any one of the seventeenth aspect to the twentieth aspect, further comprising adding at least one flavor to the mixture.
• the present disclosure pertains to the method of any one of the seventeenth aspect to the twenty-first aspect, wherein water is added to the mixture in an amount to provide a moisture % within the range of about 50% to about 80% in the plant-based cheese product.
• the present disclosure pertains to the method of any one of ihe seventeenth aspect to the twenty-second aspect, wherein the plant-based protein is present in an amount within Ihe range of about 0.01 wt% to about 15 wt% crude protein, based on a total weight of the plant-based cheese product; the stabilizer is present in an amount within the range of about 0.01 wi% to about 5 wt%, based on the fora!
• the thickening agent is present in an amount within the range of about 1 wt% to about 25 wi%, based on the total weight of the plant-based cheese product; and the fat component is present in an amount within the range of about 15 wt% to about 35 wt%, based on the total weight of hie plant-based cheese product.
• the present disclosure pertains to a method of making a plant-based cheese product comprising: adding a plant-based protein to water to form a first mixture; melting a fat component having a solid fat content within the range of about 50% to about 80% at 10 ⁇ ! C and about 15% to about 40% at lif t , adding the melted fat component, a stabilizer, and a thickening agent to the first mixture and mixing to form a second mixture; injecting steam directly into the second mixture to pasteurize the second mixture ; and homogenizing the second mixture to form the plant-based cheese product wherein hie heating by in j ecting steam may occur before or during the homogenizing.
• the present disclosure pertains to the method of the twenty- fourth aspect, further comprising adding an acidu!ent to the second mixture an amount effective to provide a pH within the range of about 3,5 to about 5.0 in the plant-based cheese product.
• the present disclosure pertains to the method of the twenty- fourth aspect or the twenty-fifth aspect, wherein the plant-based cheese product is in the form of a cream cheese product.
• the presen t disclosure pertains to the method of any one of the twenty-fourth aspect to the twenty-sixth aspect, wherein the plant-based cheese product includes no animal-derived proteins.
• the present disclosure pertains to the method of any one of the twenty-fourth aspect to the twenty-seventh aspect wherein the plant-based protein is present: in an amount within the range of about 0.01 wt% to about 15 wf % crude protein, based on a total weight of the plant-based cheese product; the stabilizer is present in an amount within tfie range of about 0.01 wf% to about 5 wt%, based on the total weight of the plant-based cheese product; the thickening agent is present in an amount within the range of about 1 wt% to about 25 wi%, based on the total weight of the plant-based cheese product; and the iat component is present in an amount within die range oi about 15 wt% to about 35 wt% , based on the total weight of the plant-based cheese product,
• a plant-based cream cheese product in the form of a homogenous mixture comprising: about 0,2 wt% to about 8 wt% plant- based crude protein, by weight of the plant-based cream cheese product; about 0.01 wt% to about 5 wt% stabilizer; about 1 wt% to about 12 wt% starch-based thickening agent; and abou t 10 wt% to about 50 wt% fat component ., wherein the fat component of Ihe plant-based cream cheese product is in the form of oil droplets with a D50 value at 40°C within the range of about 1.5 pm to about 7 pm.
• the present d isclosure pertains to the plant-based cream cheese product of the first aspect, wherein ihe fat component of the plant-based cream cheese product is in the form of oil droplets with a D50 value at 40°C within the range of about 1.5 pm to about 6.75 pm,
• the presen t disclosure pertains to the plant-based cream cheese product of the first aspect or the second aspect, wherein the fat component of the plant-based cream cheese product is in ihe form of oil droplets with a width of distribution of 5.0 pm or less.
• the present disclosure pertains to the plant-based cream cheese product oi any one of the first aspect to the third as peep wherein the fat component of the plant- based cream cheese product is in ihe form of oil droplets with a width of distribution of 4.0 pm or less.
• the present disclosure pertains to the plant-based cream cheese product of any one of the first aspect to the fourth aspect, wherein the plant-based cheese product has a complex viscosity at a frequency of 10 rad/s and a temperature of 25°C within the range of about 400 Pa ⁇ s to about 1200 Pa ⁇ s.
• the present disclosure pertains to the plant-based cream cheese product of any one of the first aspect to the fifth aspect, wherein Ihe plant-based cheese product has a complex viscosity at a frequency of 10 rad/s and a temperature of 25°C within the range of about 400 Pa ⁇ s to about 1150 Pa ⁇ s,
• the present disclosure pertains to the plant -based cream cheese product of any one of [he first aspect to the sixth aspect, wherein the plant-based cheese product has a complex viscosity at: a frequency of 10 rad/s and a temperature of 37°C within the range of about 300 Pa ⁇ s to about 1000 Pa ⁇ s.
• the present disclosure pertains to the plant-based cream cheese product of any one of the first aspect to the seventh aspect, vv herein the plant-based cheese product has a complex viscosity at a frequency of 10 rad/s and a temperature of 37°C within the range of about 300 Pa ⁇ s to about 750 Pa ⁇ s.
• the present disclosure pertains to the plant-based cream cheese product of any one of the first aspect to the eighth aspect wherein the plant-based cheese product has an elastic modulus at a temperature of 15' i ' within the range of about 4000 Pa to about 8000 Pa,
• the present; disclosure pertains to the plant-based cream cheese product of any one of the first aspect to the mneth aspect, wherein the plant-based cheese product has an elastic modulus at a temperature of 25°C within the range of about 4000 Pa to about 7500 Pa.
• the present: disclosure pertains to the plant-based cream cheese product of any one of the first aspect to the tenth aspect, vv herein the plant-based cheese product has an elastic modulus at a temperature of 37°C within the range of about 3000 Pa to about 7000 Pa,
• the presen t disclosure pertains to the plant-based cream cheese product of any one of the first aspect to the eleventh aspect, wherein the plant-based cheese product has an elastic modulus at a temperature of 37 C C within the range of about 3000 Pa to about 6000 Pa,
• the present disclosure pertains to the plant-based cream cheese product of auy one of the first aspect: to the twelfth aspect, wherein the fat component has a solid fat content; within the range of about: 50% to about 90% at 10°C and about 15% to about 45% at 20 C .
• the present disclosure pertains to the plant-based cream cheese product of any one of the first aspect to the thirteenth aspect, wherein the starch-based thickening agent is a shear tolerant starch.
• the present disclosure pertains to the piant-based cream cheese product of any one of the first aspect to the fourteenth aspect, wherein the plant-based crude protein comprises one or more of faba bean protein, pea protein, and soy protein,
• the present: disclosure pertains to the plant-based cream cheese product of tiny one of the first aspect to the fifteenth aspect, wherein the plant-based crude protein is faba bean protein.
• the present disclosure pertains to the plant-based cream cheese product of any one of the first aspect to the sixteenth aspect, wherein the fat component comprises one or more of coconut oil and sunflower oil.
• the present disclosure pertains to the piant-based cream cheese product of any one of the first aspect to the seventeenth aspect, wherein the fat component comprises coconut oil.
• the present disclosure pertains to the piant-based cream cheese product of any one of the first aspect to the eighteenth aspect, wherein the stabilizer comprises at least one hydrocolloid.
• the present disclosure pertains to the plant-based cream cheese product of any one of the first aspect to the nineteenth aspect, wherein the at least one hydrocoiloid comprises one or more of inuiin, pectin, carboxymethyiceiluiose, carrageenan, gum arabic, xanthan gum, locust bean gum, and guar gum,
• the present disclosure pertains to the piant-based cream cheese product of any one of the first aspect to the twentieth aspect wherein the at least one hydrocoiloid comprises a combination of xanthan gum, locust bean gum, and guar gum.
• the present; disclosure pertains to tire plant-based cream cheese product of any one of the first aspect to tire twenty-first aspect wherein the at least one hydrocolloid comprises locust bean gum,
• the present disclosure pertains to the plant- based cream cheese product of any one of the first; aspect to the twenty-second aspect, wherein the stabilizer is present in an amount within the range of about 0.01 wt% to about 1 wt%, based on a total weight of the plant-based cream cheese product; and the starch-based thickening agent is present in an amount within the range of about 3 wt% to about 10 wt%, based on a total weight of the plant-based cream cheese product,
• the present disclosure pertains to the plant-based cream cheese product of any one of the first aspect to the twenty-third aspect, wherein the fat component is present in an amount within the range of about 15 wt% to about 35 wt%, based ou a total weight of the plant-based cream cheese product.
• the present disclosure pertains to a making the plant-based cream cheese product according to any one of the first aspect to the twenty-fourth aspect, comprising: mixing water, a plant-based crude protein, a starch-based thickening agent a stabilizer, and a fat component to form a mixture; heating the mixture to a temperature within ihe range of about 150°F to about 20ikF; and homogenizing the heated mixture to form the plant-based cream cheese product,
• the present disclosure pertains to the method of the twenty- fifth aspect further comprising filling the plant-based cream cheese product into a container and cooling the plant-based cream cheese product to refrigeration temperature.
• the present disclosure pertains to the method of the twenty-fifth aspect; or the twenty-sixth aspect, wherein tire mixture is heated via direct: steam in j ection to a temperature within file range of about IbihF to about 20CFF for about 1 second to about 5 minutes,
• the present disclosure pertains to the method of any one of the twenty-fifth aspect; to the twenty-seventh aspect, further comprising adding an aciduiant to the mixture to provide a pH within the range of about 3.5 to 5,0 in the plant-based cream cheese product.
• the present disclosure pertains to the method of any one of the twenty-fifth aspect to tire twenty-eighth aspect, wherein water is added to the mixture in an amount to provide a rnoisture% within the range of about 50% to about 80% in the plant- based cream cheese prod uct,
• the present disclosure pertains to a method of making the plant- based cream cheese product according to any one of the first aspect to the twenty-fourth aspect, comprising; adding a plant-based protein to water to form a first mixture; melting a fat component having a solid fat content within the range of about 50% to about 90% at HTC and about 15% to about 45% at 20 ' V: adding the melted fat component, a stabilizer, and a starch- based thickening agent; to the first mixture and mixing to form a second mixture; heating the second mixture to pasteurize die second mixture; and homogenizing the second mixture to form the plan t-based cream cheese product,
• the present disclosure pertains to the method of the thirtieth aspect;, further comprising filling the plant-based cream cheese product: into a container and cooling the plant-based cream cheese product to refrigeration temperature.
• the present disclosure pertains to the method of the thirtieth aspect or the thirty-first aspect, wherein heating of the second mixture is by direct steam in j ection to a temperature within the range of about 150% ⁇ ’ to about 200°F for about 1 second to about 5 minutes.
• the present disclosure pertains to the method of any one of the thirtieth aspect to the thirty-second aspect, further comprising adding an acidulant to the first or second mixture to provide a pH within the range of about 3.5 to 5.0 in the plant-based cream cheese product.
• the present disclosure pertains to the method of any one of the thirtieth aspect to the thirty-third aspect wherein water is included in an amount to provide a moisture % within the range of about 50% to about 80% in the plant-based cream cheese product.
• ranges provided herein include the stated range and any value or sub-range within the stated range.
• a range of about 5 wi% to about 15 wt% should be interpreted to include not only the explicitly recited limits of range of about 5 wt% to aboiit 15 wt%, but; also to include Individual values, such as 6.35 wi%, 7.5 wt%, 10 wl%, 12.75 wt%, 14 wi%, etc, and sub-ranges, such as about 7 wt% to about 10.5 w ⁇ %, about 8.5 wt% to about 12.7 wt%, about 9.75 wt% to about 14 wt%, etc.
• “about” is utilized to describe a value, this is meant to encompass minor variations (up to +/- 10%) from the stated value.

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• 2022
  • 2022-06-10 JP JP2023575979A patent/JP2024532003A/ja active Pending
  • 2022-06-10 BR BR112023025825A patent/BR112023025825A2/pt unknown
  • 2022-06-10 KR KR1020247001089A patent/KR20240021258A/ko active Pending
  • 2022-06-10 AU AU2022291391A patent/AU2022291391A1/en active Pending
  • 2022-06-10 CN CN202280056369.7A patent/CN118647272A/zh active Pending
  • 2022-06-10 EP EP22821118.1A patent/EP4351350A4/en active Pending
  • 2022-06-10 US US17/837,632 patent/US20220394989A1/en active Pending
  • 2022-06-10 WO PCT/US2022/033027 patent/WO2022261450A1/en not_active Ceased
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