Classifications

• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
  • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
  • A23L2/38—Other non-alcoholic beverages
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
  • A23C11/00—Milk substitutes, e.g. coffee whitener compositions
  • A23C11/02—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins
  • A23C11/10—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
  • A23C11/00—Milk substitutes, e.g. coffee whitener compositions
  • A23C11/02—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins
  • A23C11/10—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins
  • A23C11/103—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins containing only proteins from pulses, oilseeds or nuts, e.g. nut milk
  • A23C11/106—Addition of, or treatment with, microorganisms
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
  • A23J3/00—Working-up of proteins for foodstuffs
  • A23J3/14—Vegetable proteins
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
  • A23J3/00—Working-up of proteins for foodstuffs
  • A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
  • A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
  • A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
  • A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
  • A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
  • A23L11/30—Removing undesirable substances, e.g. bitter substances
  • A23L11/33—Removing undesirable substances, e.g. bitter substances using enzymes; Enzymatic transformation of pulses or legumes
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
  • A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
  • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
  • A23L2/52—Adding ingredients
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
  • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
  • A23L2/52—Adding ingredients
  • A23L2/66—Proteins
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
  • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
  • A23L27/84—Flavour masking or reducing agents
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
  • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
  • A23L27/88—Taste or flavour enhancing agents
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
  • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
  • A23L29/06—Enzymes
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
  • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
  • A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
  • A23L5/25—Removal of unwanted matter, e.g. deodorisation or detoxification using enzymes

Definitions

• the present invention relates to a method for producing processed hemp food, beverages or food materials. Specifically, the present invention relates to a method for producing processed hemp food, beverages or food materials imparted with umami.
• Vegetable protein drinks are gaining in popularity.
• hemp milk made from hemp seed nuts is extremely nutritious.
• the protein content per 100 g of edible part is 28.8 g, which is higher than lean tuna and chicken, and the mineral content per 100 g of edible part is 11 mg of iron and 1.5 g of copper.
• At 5 mg, zinc 9.6 mg, and magnesium 690 mg it is comprehensively rich in minerals compared to sesame, almonds, liver, and oysters (Non-Patent Document 1).
• hemp milk is rich in nutritional value, including protein, it also has a unique peculiar flavor to hemp seed nuts, which is why some consumers avoid it due to its taste.
• plant-based meat also called plant meat, alternative meat
• plant-based protein which is attracting attention along with plant-based protein drinks
• the effect of treatment in the manufacturing process of beverages, foods, and food materials containing hemp protein on taste has not been sufficiently studied.
• the object of the present invention is to provide a processing technology that can improve the taste of hemp food or food materials.
• the present inventor unexpectedly discovered that umami is added to hemp food, beverages or food materials by treating them with filamentous fungus-derived proteases.
• the present invention has been completed through further studies based on these findings.
• Section 1 A method for producing a processed hemp food, drink, or food material, comprising a step of treating a hemp food, drink, or food material with a filamentous fungus-derived protease.
• Section 2. Item 2. The production method according to Item 1, wherein the filamentous fungus-derived protease is an Aspergillus-derived protease.
• Item 3. Item 3. The production method according to Item 1 or 2, wherein the filamentous fungus-derived protease is Aspergillus oryzae-derived protease.
• Section 4. Item 4. The production method according to any one of Items 1 to 3, wherein the hemp food, drink or food material is hemp milk.
• Item 5. A flavor-imparting agent for hemp food, beverages or food materials, containing a filamentous fungus-derived protease.
• a processing technology capable of imparting umami to hemp food, beverages or food materials.
• the process for producing the processed hemp food, drink or food material of the present invention is characterized by including a step of treating a hemp food, drink or food material with a filamentous fungus-derived protease.
• a step of treating a hemp food, drink or food material with a filamentous fungus-derived protease is characterized by including a step of treating a hemp food, drink or food material with a filamentous fungus-derived protease.
• hemp food, drink or food material used in the present invention is a beverage containing hemp protein (drinkable, hemp drink) or a food containing hemp protein (edible, hemp food).
• "food” includes not only food for humans but also feed for animals other than humans.), or food materials containing hemp protein (combined with other food materials, beverages or a food material that constitutes a food, a hemp food material.).
• the beverage containing hemp protein is not particularly limited as long as it is a liquid in which hemp protein is dissolved and/or dispersed in water.
• beverages containing hemp protein include (i) a liquid obtained by dispersing a dry powder of a food material containing hemp protein in water; (ii) crushing a food material containing hemp protein in water; and a liquid obtained by dispersing; (iii) a liquid obtained by removing at least a part of the components other than hemp protein from the liquid (i) or (ii) above to increase the hemp protein content; (iv) Liquids obtained by dissolving and/or dispersing dry powder prepared from any one of the above liquids (i) to (iii) in water.
• Food materials containing hemp protein are usually hemp seed nuts (hemp seeds with the seed coat removed), but other examples include hemp seeds, hemp seed pomace (the part without the hemp oil). Certain hemp protein particles or hemp protein powders are also included.
• a preferred example of a beverage containing hemp protein is hemp milk, which is an aqueous dispersion of crushed hemp seed nuts.
• Foods containing hemp protein include, for example, textured hemp protein.
• the textured hemp protein is a product that is textured with an extruder or the like using hemp seeds, pomace obtained by removing hemp oil from hemp seeds, etc. as a raw material, and has a texture close to that of meat.
• the term “hemp” in the present invention refers to so-called industrial hemp, and specifically refers to a variety that does not contain THC (tetrahydrocannabinol) or has a low THC concentration that causes perceptual alteration. Because industrial hemp does not contain THC or its low concentration, it has no efficacy as a sensory-altering drug and cannot lead to abuse.
• Food materials containing hemp protein include, for example, dry powder of food materials containing hemp protein, hemp seed pomace (hemp protein particles or hemp protein powder), and the like.
• the protein content in the hemp food or food material used in the present invention is not particularly limited, but is, for example, 0.1% by weight or more, preferably 0.5% by weight or more, more preferably 1% by weight or more, and 2% by weight. Above, 3% by weight or more, 4% by weight or more, or 5% by weight or more.
• the upper limit of the protein content range in the hemp food or food material is, for example, 11% by weight or less, preferably 10% by weight or less, more preferably 9% by weight or less, further preferably 8% by weight or less, 7% by weight or less, More preferably, it is 6% by weight or less.
• the upper limit of the protein content range is preferably applied particularly when the hemp food or food material is a beverage containing hemp protein.
• filamentous fungus-derived protease used in the present invention is not particularly limited as long as it is an enzyme derived from filamentous fungi that hydrolyzes peptide bonds of proteins.
• the filamentous fungus-derived protease imparts umami to hemp food, beverages or food materials.
• proteases derived from filamentous fungi include Aspergillus genus, Mucor genus, Neurospora genus, Penicillium genus, Rhizomucor genus, Rhizopus genus, and Sucre Examples include proteases derived from the genus Sclerotinia and the like.
• One type of these filamentous fungus-derived proteases may be used alone, or a plurality of types may be used in combination.
• Aspergillus-derived proteases are preferred from the viewpoint of further enhancing the umami-imparting effect.
• Aspergillus-derived proteases include Aspergillus oryzae, Aspergillus niger, Aspergillus melleus, Aspergillus japonicus, Aspergillus awamori, ⁇ (Aspergillus kawachii) ⁇ (Aspergillus sojae) ⁇ (Aspergillus tamarii) ⁇ (Aspergillus foetidus) ⁇ (Aspergillus fumigatus) ⁇ (Aspergillus nidulans) ⁇ (Aspergillus aculeatus) ⁇ (Aspergillus candidus) ⁇ (Aspergillus flavus) ⁇ (Aspergillus saitoi) ⁇ (Aspergillus inuii) ⁇ (Aspergillus glaucus) ⁇ (Aspergillus caesiellus) ⁇ (Aspergillus clavatus) ⁇ (Aspergillus deflectus) ⁇ (Aspergillus fischerianus)
• Aspergillus-derived proteases may be used singly or in combination. Among these Aspergillus-derived proteases, Aspergillus oryzae-derived proteases are preferred from the viewpoint of further enhancing the umami-imparting effect.
• a filamentous fungus-derived protease can be prepared by a known method.
• a protease derived from the genus Aspergillus it can be easily prepared by a method of koji-making Aspergillus spp. and separating the protease using a known means, a method using genetic recombination technology, or the like.
• proteases derived from filamentous fungi include Proteax (protease derived from Aspergillus oryzae), PR-AN100 (neutral protease derived from Aspergillus oryzae), protease HF "Amano" 150SD (manufactured by Amano Enzyme Co., Ltd.).
• Aspergillus oryzae-derived acid protease Protease M “Amano” (Aspergillus oryzae-derived acid protease), Acid protease UF “Amano” SD (Aspergillus niga-derived acid protease), Protease A “Amano” (Aspergillus oryzae-derived acid protease) neutral protease from Aspergillus), Protease A “Amano” 2SD (neutral protease from Aspergillus oryzae); Sumiteam MP (alkaline protease from Aspergillus meleus), Sumiteam FL-G (Aspergillus) from Shinnihon Kagaku Kogyo Co., Ltd. - Oryzae-derived alkaline protease) and the like.
• the amount of filamentous fungus-derived protease used is not particularly limited, but the amount used per 1 g of hemp protein is, for example, 0.1 U or more. From the viewpoint of further enhancing the umami-imparting effect, the amount of filamentous fungus-derived protease used per 1 g of hemp protein is preferably 1 U or more, more preferably 5 U or more, 10 U or more, 100 U or more, 200 U or more, 300 U or more, or 400 U or more. is mentioned.
• the upper limit of the amount of filamentous fungus-derived protease used per gram of hemp protein is not particularly limited, but is preferably 2000 U or less, more preferably 1800 U or less, still more preferably 1600 U or less, still more preferably 1500 U or less, 1300 U or less, or 1100 U or less. , 900 U or less, 700 U or less, 500 U or less, 300 U or less, 200 U or less, 100 U or less, 50 U or less, or 10 U or less.
• the amount of filamentous fungus-derived protease used per 1 g of hemp seed is, for example, 0.1 U or more.
• the amount of filamentous fungus-derived protease used per 1 g of hemp seed is preferably 1 U or more, more preferably 3 U or more, still more preferably 5 U or more, 10 U or more, 100 U or more, 200 U or more, Or 300U or more is mentioned.
• the upper limit of the amount of filamentous fungus-derived protease used per 1 g of hemp seed is not particularly limited, but is preferably 1500 U or less, more preferably 1200 U or less, further preferably 1000 U or less, 800 U or less, 600 U or less, 400 U or less, and 300 U or less. , 200 U or less, 100 U or less, 50 U or less, or 10 U or less.
• the protease activity shall be measured by the Folin method using casein as a substrate. That is, the activity of protease is defined as 1 unit (1U) of the amount of enzyme that causes an increase in Folin's test solution coloring substance equivalent to 1 ⁇ g of tyrosine per minute in an enzymatic reaction using casein as a substrate in a conventional manner.
• hemp food product or food material with a filamentous fungus-derived protease, such as reaction conditions
• the hemp food product or food material and the filamentous fungus-derived protease are added to the hemp food product or food material by adding the filamentous fungus-derived protease.
• a hemp food/beverage product or food material composition containing the hemp is prepared, and the hemp food/beverage product or food material composition is maintained in a heated state to allow the umami-producing reaction to proceed.
• the treatment temperature with the filamentous fungus-derived protease is not particularly limited, and can be appropriately determined by those skilled in the art depending on the optimum temperature of the enzyme used and/or the thermal properties of the hemp food or food material. 70°C, preferably 48-62°C.
• the treatment reaction time with the filamentous fungus-derived protease is not particularly limited, and may be appropriately determined according to the preparation scale of the composition, the timing of addition of the enzyme, etc. For example, it is 30 minutes or more, preferably 50 minutes or more. .
• the upper limit of the treatment reaction time range is not particularly limited, but includes, for example, 12 hours or less, 8 hours or less, or 4 hours or less.
• the hemp food or beverage or food material composition is subjected to enzyme deactivation treatment, cooled, and subjected to post-treatment such as filtration as necessary to obtain a processed hemp food or beverage or food material.
• the processed hemp food, drink, or food material obtained by the production method of the present invention is imparted with umami, and can be provided as a highly palatable food.
• a preferred processed hemp food, drink or food material is a beverage (processed hemp drink), and particularly preferably processed hemp milk.
• the obtained processed hemp food or beverage or food material may be prepared as a dried processed hemp food or food material through a drying process.
• the processed hemp food, drink or food material is a beverage
• it can be prepared as a solid processed hemp composition that, when dissolved and/or dispersed in water, yields a hemp beverage imparted with umami.
• the method of drying is not particularly limited, and examples thereof include freeze drying, vacuum drying, spray drying and the like.
• Forms of solid processed hemp compositions include powders, granules, granules, and the like.
• the filamentous fungus-derived protease can impart umami to hemp, food, beverage, or food material. Therefore, the present invention also provides a flavor-imparting agent for hemp, food, beverages or food materials containing a filamentous fungus-derived protease.
• a trichloroacetic acid test solution (containing 1.8% (w/v) trichloroacetic acid, 1.8% (w/v) sodium acetate and 0.33 mol/L acetic acid [PR -AX, TH-PC10F, PR-NY, PR-AY], or containing 0.44 mol/L trichloroacetic acid [PR-AN100]) is added, shaken, and left at 37°C for 30 minutes. and filtered.
• the amount of enzyme that causes an increase in Folin's test solution coloring substance equivalent to 1 ⁇ g of tyrosine per minute was defined as 1 unit (1 U).

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• Life Sciences & Earth Sciences (AREA)
• Chemical & Material Sciences (AREA)
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• Food Science & Technology (AREA)
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• Microbiology (AREA)
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• 2022
  • 2022-04-05 WO PCT/JP2022/017088 patent/WO2022215689A1/ja not_active Ceased
  • 2022-04-05 CN CN202280017833.1A patent/CN117642080A/zh active Pending
  • 2022-04-05 EP EP22784671.4A patent/EP4321036A4/en active Pending
  • 2022-04-05 US US18/553,945 patent/US20240215595A1/en active Pending
  • 2022-04-05 JP JP2023513019A patent/JPWO2022215689A1/ja active Pending

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