WO2022209074A1 - Système d'analyse, procédé d'analyse et programme pour système d'analyse - Google Patents

Système d'analyse, procédé d'analyse et programme pour système d'analyse Download PDF

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Publication number
WO2022209074A1
WO2022209074A1 PCT/JP2021/047820 JP2021047820W WO2022209074A1 WO 2022209074 A1 WO2022209074 A1 WO 2022209074A1 JP 2021047820 W JP2021047820 W JP 2021047820W WO 2022209074 A1 WO2022209074 A1 WO 2022209074A1
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WIPO (PCT)
Prior art keywords
sample
liquid
sample holder
range
setting
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PCT/JP2021/047820
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English (en)
Japanese (ja)
Inventor
悠佑 長井
慧 若林
裕之 北村
Kayo MORINAGA (森長 佳世)
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株式会社島津製作所
株式会社堀場製作所
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Priority to JP2023510260A priority Critical patent/JPWO2022209074A1/ja
Publication of WO2022209074A1 publication Critical patent/WO2022209074A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/74—Optical detectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/80—Fraction collectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/86—Signal analysis

Definitions

  • the present invention relates to an analysis system, analysis method, and analysis system program using liquid chromatography.
  • a liquid sample is separated and analyzed by an apparatus (liquid chromatograph) using the principle of liquid chromatography, and the liquid sample is separated using a fraction collector.
  • an analyzer using another principle such as Raman spectroscopy.
  • the main object of the present invention is to provide a user-friendly analysis system in which it is easy to grasp the correspondence between the range of fractionation of the liquid sample by the fraction collector and the dropping position.
  • the analysis system includes a liquid chromatograph that separates and analyzes a liquid sample for each component, separates the liquid sample that has passed through the liquid chromatograph, and divides the separated liquid into a plurality of samples.
  • a fraction collector that drips onto a sample holder having a holder, an analyzer that analyzes a sample component contained in the fractionated liquid dripped onto each of the sample holders, and the fraction collector among the plurality of sample holders.
  • a setting screen display unit for displaying, on a display, a dropping range setting screen for setting the range of the sample holding unit to which the fractionated liquid is dropped, wherein the setting screen display unit displays the sorting range of the liquid sample;
  • a setting chromatogram for setting and a sample holder symbol having a plurality of sample holder symbols simulating the sample holder are simultaneously displayed on the dropping range setting screen, and on the setting chromatogram A collection range of the liquid sample is displayed, and the sample holder symbol corresponding to the collection range is displayed on the sample holder symbol.
  • the setting chromatogram of the liquid sample and the sample holder symbol simulating the sample holder are displayed simultaneously on one screen, and the preparative collection range of the liquid sample is displayed on the setting chromatogram.
  • the sample holder symbol corresponding to this fractionation range is displayed on the sample holder symbol, the user can easily determine the fractionation range of the liquid sample and the position of the sample holder onto which the fractionated liquid is dropped. It is possible to visually comprehend the correspondence relationship between , and to set the operation of the fraction collector more easily than before.
  • the sample holder symbol corresponding to the selected fractionation range is displayed on the sample holder symbol.
  • the sample holder symbol is displayed so as to be distinguishable from other sample holder symbols that do not correspond to the selected preparative collection range, and when the user selects the sample holder symbol on the sample holder symbol, the selected It is preferable that the preparative collection range corresponding to the sample holder symbol is displayed on the setting chromatogram. In this way, it is possible to grasp how much the sample holder is used for the fractionation range of the selected liquid sample. Furthermore, it is possible to grasp which range of the liquid sample is dropped onto the selected sample holder.
  • the analysis system further includes a storage unit that stores drop start position information regarding the position of the sample holding unit where dropping of the preparative liquid starts, and dropping amount information concerning the amount of dropping onto each sample holding unit. and determining the sample holder onto which the preparative liquid is dropped based on the preparative collection range on the setting chromatogram selected by the user and the stored drop start position information and drop amount information. , the sample holder symbol on the sample holder symbol. In this way, when setting the operation of the fraction collector, it is possible to grasp in more detail how much the sample holder is used.
  • the analysis system displays an error message on the display when the number of the sample holders determined by the dropping range determination unit exceeds the number of the sample holder symbols that can be displayed in the sample holder symbol. is preferably displayed. In this way, the user can understand that the set information on the drip start position and the drip amount is not appropriate, and usability can be further improved.
  • the user moves a bar displayed on the setting chromatogram to select the fractionation range of the liquid sample, and in conjunction with the movement of the bar, It is preferable that the specimen holding portion symbol displayed is configured to change its form.
  • the aspect of the sample holder symbol on the sample holder symbol changes, so that the range of fractionation of the liquid sample and the amount of liquid to be fractionated change. It is possible to make it easier to visually grasp the corresponding relationship with the sample holding portion to which the sample is dropped.
  • the analysis system further includes an analysis result display unit for displaying an analysis result screen showing analysis results
  • the analysis result display unit includes an analysis chromatogram, which is an analysis result of the liquid sample by the liquid chromatograph, and the sample.
  • the sample holder symbol corresponding to the selected fractionation range is displayed on the sample holder symbol.
  • the fractionation range is displayed on the setting chromatogram. In this way, when reviewing the analysis results, it is possible to easily grasp the correspondence relationship between the fractionation range in the chromatograph obtained by the analysis and the sample holder into which the fractionated liquid was dropped.
  • the analysis result display unit further displays the analysis result of the analysis device on the analysis result screen.
  • the analysis device analyzes by Raman spectroscopy.
  • the sample components contained in the fractionated liquid separated by the liquid chromatograph are analyzed by Raman spectroscopy. It is possible to detect extremely small amounts of components that cannot be detected by conventional methods.
  • sample holder is preferably a plate having a plurality of wells formed as the sample holder. In this way, it can be used more preferably in combination with an analysis device using Raman spectroscopy.
  • the analysis method includes a liquid chromatograph for separating and analyzing a liquid sample for each component, fractionating the liquid sample that has passed through the liquid chromatograph, An analysis method using a fraction collector that drips onto a sample holder having a sample holder, and an analyzer that analyzes sample components contained in the fractionated liquid dropped onto each of the sample holders, wherein the plurality of Displaying on the display a dropping range setting screen for setting the range of the sample holding part to which the fraction collection liquid is dropped by the fraction collector among the sample holding parts of No., and for setting the fractionation range of the liquid sample A setting chromatogram and a sample holder symbol having a plurality of sample holder symbols simulating the sample holder are simultaneously displayed on the dropping range setting screen, and fractionation of the liquid sample is performed on the setting chromatogram. The range is displayed, and the sample holder symbol corresponding to the preparative collection range is displayed on the sample holder symbol.
  • the analysis system program includes a liquid chromatograph for separating and analyzing a liquid sample for each component, fractionating the liquid sample that has passed through the liquid chromatograph, and
  • a program used in an analysis system comprising: a fraction collector that drips onto a sample holder having a plurality of sample holders; a setting screen display unit for displaying on a display a dropping range setting screen for setting a range of the sample holding part to which the fraction collector drops the fractionated liquid among the plurality of sample holding parts.
  • a setting chromatogram for setting the fractionation range of the liquid sample and a sample holder symbol having a plurality of sample holder symbols simulating the sample holder are displayed on the computer, and the setting screen display unit displays the is simultaneously displayed on the dropping range setting screen, the fractionation range of the liquid sample is displayed on the setting chromatogram, and the sample holder symbol corresponding to the fractionation range is displayed on the sample holder symbol. It is characterized by displaying.
  • FIG. 1 is a schematic diagram showing the overall configuration of an analysis system in one embodiment of the present invention
  • FIG. The functional block diagram which shows the function of the integrated management apparatus in the same embodiment.
  • the analysis system 100 of this embodiment performs LC-Raman analysis using both liquid chromatography and Raman spectroscopy, and is a type of so-called hyphenated technology.
  • this analysis system includes a liquid chromatograph 10, a fraction collector 20, and a Raman spectroscopic analysis device 30, and integrated management of setting of these operations, data analysis, display of analysis results, etc. and an integrated management device 40 that
  • the liquid chromatograph 10, the fraction collector 20, and the Raman spectroscopic analysis device 30 are equipped with control calculators 1C, 2C, and 3C, which are dedicated software for performing respective hardware operations and data analysis.
  • the integrated management device 40 is configured to operate as overlay software, and the integrated management device 40 and the control calculators 1C, 2C, and 3C of each device cooperate to operate integrally as one analysis system 100. do.
  • the liquid chromatograph 10 separates and detects each component of the liquid sample S by liquid chromatography.
  • the liquid chromatograph 10 As shown in FIG. By feeding the liquid sample S together with the phase Z to the separation column 14, the liquid sample S is separated for each component.
  • a component detector 15 for detecting separated components of the liquid sample S is provided downstream of the separation column 14 .
  • the mobile phase Z is, for example, a mixed liquid in which a plurality of types of liquids are mixed, and here is a mixed liquid of water and an organic solvent such as ethanol.
  • the mobile phase Z may consist of a single liquid, or may be a gradient solvent having a concentration gradient.
  • the liquid chromatograph 10 further includes an LC control calculator 1C that controls each device such as the pump P and generates a chromatogram of the liquid sample S based on the output of the component detector 15.
  • the LC control arithmetic unit 1C is composed of a dedicated or general-purpose computer, executes a chromatographic program stored in a memory, and realizes its functions through the cooperation of each device.
  • the fraction collector 20 is provided on the downstream side of the component detector 15 of the liquid chromatograph 10 as shown in FIG.
  • the fraction collector 20 is mounted on a plate PL (sample holder in the claims) in which a plurality of wells (sample holders in the claims) W are formed in a matrix.
  • a plate PL sample holder in the claims
  • W sample holders in the claims
  • the fractional liquid SS is not limited to a mobile phase Z containing components derived from the liquid sample S. , the concept includes those consisting only of the mobile phase Z.
  • the configuration of the fraction collector 20 will be described in detail. It is configured to drop SS in predetermined amounts one after another.
  • the fraction collector 20 also includes a stacker 24 that stores a plurality of plates PL. This stacker 24 is for waiting the plate PL before the separation liquid SS is dropped into the well W and the plate PL until the separation liquid SS is dropped into the well W and dried (dried). is. A maximum of three plates PL can be placed on each of the three racks A, B, and C of the stacker 24 . Movement of the plate PL between the stacker 24 and the stage 22 is performed by a transport mechanism (not shown).
  • the fraction collector 20 is further provided with a fraction control calculator 2C that controls the mobile probe 21 and the like and generates fraction information, which is information about the state of dropping into each well W of each plate PL.
  • the fraction control arithmetic unit 2C is composed of a dedicated or general-purpose computer, executes a chromatographic program stored in a memory, and realizes its functions through the cooperation of each device.
  • the fraction control computing unit 2C determines the position of the mobile probe 21 and the fractionation conditions (fractionation flow rate, fraction It has at least a fraction control section 2C1 for controlling the time taken, etc.).
  • the fraction control computing unit 2C based on the fraction setting information input from the integrated management device 40, predicts the fraction result, which is the usage range of the wells W in the plate PL when the preparative liquid SS is dropped, and the plate A fraction information generation unit 2C2 is further provided for generating fraction information such as a fraction result when the preparative liquid SS is actually dripped onto the PL. Data relating to the fraction information generated by the fraction information generating section 2C2 is transmitted to the integrated management device 40.
  • the integrated management device 40 generates and stores a correspondence relationship between the position of the well W into which the preparative liquid SS is dropped and the area on the chromatogram.
  • the Raman spectroscopic analyzer 30 analyzes the sample components contained in the sampled liquid SS dropped in the wells W on the plate PL in a dried state based on Raman spectroscopy.
  • the plate PL the user carries it to the Raman spectroscopic analyzer 30 after it has been dried in the fraction collector 20.
  • a plate PL may be transported.
  • the Raman spectroscopic analysis apparatus 30 includes a light irradiator 31 that irradiates excitation light such as laser light to the wells W on the plate PL holding the preparative liquid SS, and a light irradiator 31 that irradiates the excitation light.
  • the Raman spectroscopic analyzer 30 controls the position on the plate PL where the laser light emitted by the light irradiator 31 is irradiated, and Raman control for generating a Raman spectrum based on the output of the Raman scattered light detector 33.
  • a calculator 3C is further provided.
  • the Raman control computing unit 3C is composed of a dedicated or general-purpose computer, executes a program dedicated to the Raman spectroscopic analysis apparatus stored in memory, and realizes its functions through the cooperation of each device.
  • the integrated management device 40 is connected to the LC control computing unit 1C, the fraction control computing unit 2C, and the Raman control computing unit 3C via a wired or wireless network, and has various functions between the control computing units 1C, 2C, and 3C. It transmits and receives information, and is specifically composed of a dedicated or general-purpose computer equipped with a CPU, memory, and the like.
  • the integrated management device 40 transmits setting information (chromatograph setting information, fraction setting information, Raman spectroscopic analysis setting information) for setting parameters related to analysis to each of the control calculators 1C, 2C, and 3C, It also receives information on analysis results obtained from the respective control calculators 1C, 2C, and 3C, results of operations performed on the plate PL, and the like.
  • setting information chromatograph setting information, fraction setting information, Raman spectroscopic analysis setting information
  • the integrated management device 40 cooperates with the CPU and other peripheral devices in accordance with the analysis system program stored in the memory, thereby providing an input receiving section 41, a setting screen display section, and a setting screen display section as shown in FIG. 42 , setting information generation unit 43 , storage unit 44 and analysis result display unit 45 at least.
  • the input reception unit 41 receives various inputs from the user through input devices such as keyboards and mice.
  • the setting screen display unit 42 displays a setting screen regarding each of the devices 10, 20, and 30 on the display DP according to the input from the user.
  • the setting screen display unit 42 displays a plate selection screen (not shown) for setting which plate PL set in the stacker 23 is to be used for sorting the liquid sample S, and a plate selection screen (not shown) shown in FIG.
  • a dropping range setting screen 50 or the like for setting the dropping range of the fraction collector 20 (that is, the range in which the sorting liquid SS is dropped among the plurality of wells W on the plate PL) is displayed on the display DP. to display.
  • the user can set the devices 10, 20, and 30 by inputting information on various setting screens displayed on the display DP.
  • the setting information generation unit 43 generates chromatograph setting information, fraction setting information, and Raman spectroscopic analysis setting information related to analysis based on the user's input to the setting screen, and transmits them to each of the devices 10, 20, and 30. It is.
  • the setting information generating unit 43 when the user inputs necessary information on the dripping range setting screen 50 and approves the dripping setting, the setting information generating unit 43 generates fraction setting information such as fractionation parameters to be transmitted to the fraction collector 20. . Then, the generated fraction setting information is transmitted from the integrated management device 40 to the fraction control computing unit 2C, and each device of the fraction collector 20 is controlled by the fraction control unit 2C1 to dispense the sample components onto the actual plate PL. is done.
  • the setting information generation unit 43 generates setting information (chromatograph setting information) of the liquid chromatograph 10 and Raman spectroscopic analysis based on the user's input to the setting screen of the liquid chromatograph 10 and the setting screen of the Raman spectroscopic analysis device 30 .
  • Setting information (Raman spectroscopic analysis setting information) of the device 30 is generated and transmitted to the corresponding devices 10 and 30 .
  • the storage unit 44 is set in a predetermined area of the memory, and stores various information input by the user and various setting information (chromatograph setting information, fraction setting information, Raman spectroscopy information) generated by the setting information generation unit 43.
  • analysis setting information chromatograph setting information, fraction setting information, Raman spectroscopy information
  • This operation result includes fraction information such as a fraction result.
  • the analysis result display unit 45 displays an integrated analysis result screen 60 on the display DP based on the information recorded in the storage unit 44 .
  • the analysis result display unit 45 refers to the analysis results and operation results received from the devices 10, 20, and 30 recorded in the storage unit 44, and generates, for example, an analysis result screen 60 shown in FIG. and displayed on the display DP.
  • On this analysis result screen 60 at least the chromatogram (analysis chromatogram) of the liquid sample, which is the analysis result of the liquid chromatograph 10, and the Raman spectrum of the sample component, which is the analysis result of the Raman spectrometer 30, are displayed side by side. be done.
  • each device 10, 20, 30 necessary for starting analysis only by inputting to the integrated management device 40, and each device 10, 20, 30 A series of analyzes can be performed without operating dedicated software, and the analysis results can be confirmed.
  • the setting screen display unit 42 displays a setting chromatogram 51 for setting the preparative collection range of the liquid sample S and a plate symbol S PL imitating the plate PL (as the sample holder symbol in the claims). equivalent) are displayed side by side on the dropping range setting screen 50 at the same time, and the separation range of the liquid sample S is displayed on this setting chromatogram 51, and the separation liquid SS is dropped corresponding to this separation range.
  • a well symbol S W (corresponding to a sample holder symbol in the scope of claims) that imitates the well W in which the plate is located is displayed on the plate symbol S PL .
  • the setting chromatogram 51 is based on, for example, a chromatogram that is an analysis result of another liquid sample having similar sample components to the liquid sample S to be analyzed, or a past analysis result of the liquid chromatograph 10. It is a created model or the like, which is input in advance by the user and stored in the storage unit 44 .
  • Two bars 52 are displayed on the setting chromatogram 51 to indicate the fractionation range of the liquid sample S (also referred to as the fraction period).
  • the area between the two bars 52 indicates the sorting range.
  • the user can select the fractionation range by moving the positions of the two bars 52 on the setting chromatogram 51 by a drag operation or the like.
  • the plate symbol S PL is a schematic plan view of the plate PL to be used.
  • a plurality of well symbols SW imitating a plurality of wells W are displayed in a matrix so as to have the same number and arrangement as the actual plate PL .
  • a predicted dropping range of the sample liquid SS is displayed according to the sample collection range displayed on the setting chromatogram 51.
  • FIG. Specifically, as shown in FIG. 3, among a plurality of well symbols SW , the mode (color, size, brightness, etc.) of the well symbol SW predicted to drop the sample liquid SS is changed.
  • the drop range on the plate PL is indicated by .
  • such a dropping range setting screen 50 when the user selects the fractionation range of the liquid sample S on the setting chromatogram 51, the mode of each well symbol SW on the plate symbol SPL is changed accordingly.
  • the well symbol SW corresponding to the selected fractionation range is displayed so as to be distinguishable from other well symbols Sw .
  • the position of the bar 52 moves accordingly on the setting chromatogram 51, and the selected well symbol S
  • the fractional collection range of the liquid sample S corresponding to W is displayed.
  • this dropping range setting screen 50 information (dropping start position information) on the position of the well W where dropping of the preparative liquid SS is started, and the amount (or dropping time) of dropping from the mobile probe 21 to each well W are displayed.
  • Input boxes B1 and B2 are provided for inputting information (dripping amount information) regarding.
  • the dropping range displayed on the plate symbol SPL is changed in consideration of these information. That is, in this embodiment, the well W to which the preparative liquid SS is dropped is determined based on the preparative collection range on the setting chromatogram 51 selected by the user and the input dropping start position information and dropping amount information.
  • a range (number) is determined and configured to be reflected on the plate symbol S PL . If the determined number of wells W exceeds the number that can be displayed on the plate symbol SPL , the dropping range setting screen 50 is configured to display an error message. Then, when the setting of the dripping range is confirmed by the user's operation such as clicking the confirm button B3 displayed on the dripping range setting screen 50, information on the setting of the dripping range (dripping position, dripping amount, drop start position, start time, etc.) are stored in the storage unit 44 .
  • the analysis when confirming the analysis result, the analysis is performed in order to easily grasp the correspondence relationship between the fractionation range of the liquid sample S and the well W into which the fractionation liquid SS was dropped.
  • the result display unit 45 refers to the information about the dropping position stored in the storage unit 44, and the analysis chromatogram 61, which is the analysis result of the liquid sample S by the liquid chromatograph 10, and the plate symbol S simulating the plate PL.
  • PL and PL are simultaneously displayed on the analysis result screen 60, the fractionation range of the liquid sample S is displayed on this analysis chromatogram 61, and the well W into which the fractionation liquid SS is dropped corresponding to this fractionation range is displayed. It is configured to be displayed on the plate symbol SPL .
  • the plate symbol S PL displayed on the analysis result screen 60 has the same aspect as the plate symbol S PL displayed on the dropping range setting screen 50 .
  • this analysis result screen 60 when the user selects an arbitrary preparative range on the analysis chromatogram 61, the aspect (for example, color) of each well symbol SW on the plate symbol SPL changes accordingly.
  • the well symbol SW corresponding to the selected preparative collection range is displayed so as to be distinguishable from other well symbols SW .
  • a corresponding bar is displayed on the analytical chromatogram.
  • 62 is displayed, and the collection range corresponding to the selected well symbol SW is displayed.
  • the analysis result of the Raman spectroscopic analyzer 30 is also displayed on the analysis result screen 60 at the same time. Specifically, when one well symbol SW is selected by the user from the plate symbol SPL , the Raman spectrum and the microscopic image of the sample liquid SS dropped in the well corresponding to the well symbol SW are displayed. An image 64 is displayed.
  • the setting chromatogram 51 of the liquid sample S and the plate symbol S PL are simultaneously displayed on the dropping range setting screen 50, and the liquid sample S is displayed on the setting chromatogram 51.
  • the fractionation range of the sample S is displayed, and the well W from which the fractionation liquid SS corresponding to this fractionation range is fractionated is displayed on the plate symbol SPL .
  • the relationship between the range of fractionation and the position of the well W into which the fractionation liquid SS is dropped can be visually grasped easily, and the operation setting of the fraction collector 20 can be performed more easily than before.
  • the well symbol SW corresponding to the selected fractionation range is changed to another well symbol S on the plate symbol SPL .
  • the fractionation range corresponding to the selected well symbol SW is displayed on the setting chromatogram 51. Therefore, it is possible to grasp how many wells W are used for the selected fractionation range of the liquid sample S, and to what extent the liquid sample S is fractionated for the wells W on the plate. It can be grasped whether SS is dropped.
  • the analysis chromatogram 61 of the liquid sample S and the plate symbol S PL are displayed at the same time. Since the well W in which the preparative solution SS corresponding to . It is possible to easily grasp the correspondence relationship with the well W into which the liquid SS is dropped.
  • the present invention is not limited to the above embodiments.
  • the analysis chromatogram 61 and the plate symbol SPL are displayed side by side on the analysis result screen 60, but the present invention is not limited to this. In other embodiments, the plate symbol S PL may not be displayed on the analysis result screen 60 .
  • the effects of the present invention can be achieved.
  • the user moves the position of the bar 52 displayed on the setting chromatogram 51 on the dropping range setting screen 50 to set the collection range of the liquid sample S.
  • the collection range may be set by inputting the start time and end time of the fraction as numerical values in the input boxes provided on the dropping range setting screen 50 .
  • the analysis system 100 of the present invention only needs to be configured to separate the liquid sample S by liquid chromatography, and does not necessarily need to include the component detector 15 constituting the liquid chromatograph 10, for example. Further, the analysis system 100 of the present invention does not necessarily have to include the Raman spectroscopic analysis device 30, and the liquid sample S separated by liquid chromatography is analyzed by, for example, infrared spectroscopy, nuclear magnetic resonance, or time-of-flight mass spectroscopy. It may be provided with an analysis device that performs analysis using the principle of an analysis method or the like.
  • the preparative liquid SS is dropped into a plurality of wells W formed on the plate PL, but the present invention is not limited to this.
  • the fractionated liquid SS may be dropped onto a sample holder provided with a plurality of sample holders collectively.
  • a sample holder is a test tube rack that uses an independent container such as a test tube as the sample holder, and that can accommodate a plurality of containers and these containers in a regularly arranged state. and the like can be exemplified.
  • the sample holder in which a plurality of sample holders are integrated is not limited to a plate having wells as described above. A plate or the like may be used.
  • each sample holder may not be integrally fixed.
  • the effect of the present invention can be more pronounced as the number increases, such as 50 or more, 100 or more, or 200 or more. can.
  • the present invention it is possible to provide a user-friendly analysis system in which it is easy to grasp the correspondence between the liquid sample collection range by the fraction collector and the dropping position.

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Abstract

L'invention concerne un système d'analyse équipé d'un chromatographe en phase liquide pour séparer un échantillon liquide en composants et effectuer une analyse de celui-ci, d'un collecteur de fraction qui fractionne l'échantillon liquide ayant traversé le chromatographe liquide et qui verse un liquide fractionné ayant subi un fractionnement vers un corps de support d'échantillon ayant une pluralité de parties de maintien d'échantillon; d'un dispositif d'analyse qui analyse les composants d'échantillon contenus dans le liquide fractionné, qui a été versé sur les parties de maintien d'échantillon; et d'une partie d'affichage d'écran de réglage pour afficher, sur un dispositif d'affichage, un écran de réglage de plage de dépôt pour régler une plage de parties de maintien d'échantillon, qui sont parmi la pluralité de parties de maintien d'échantillon et sur lesquelles un liquide fractionné doit être versé par le collecteur de fraction. La partie d'affichage d'écran de réglage affiche simultanément, sur l'écran de réglage de plage de dépôt, un chromatogramme de réglage pour régler une plage de fractionnement de l'échantillon liquide et un symbole de corps de maintien d'échantillon ayant une pluralité de symboles de partie de maintien d'échantillon imitant les parties de maintien d'échantillon, affiche la plage de fractionnement de l'échantillon liquide sur le chromatogramme de réglage, et affiche, sur le symbole de corps de maintien d'échantillon, les symboles de partie de maintien d'échantillon correspondant à la plage de fractionnement.
PCT/JP2021/047820 2021-03-30 2021-12-23 Système d'analyse, procédé d'analyse et programme pour système d'analyse WO2022209074A1 (fr)

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