WO2022209074A1

WO2022209074A1 - Analysis system, analysis method, and program for analysis system

Info

Publication number: WO2022209074A1
Application number: PCT/JP2021/047820
Authority: WO
Inventors: 悠佑長井; 慧若林; 裕之北村; Kayo MORINAGA (森長佳世)
Original assignee: 株式会社島津製作所; 株式会社堀場製作所
Priority date: 2021-03-30
Filing date: 2021-12-23
Publication date: 2022-10-06
Also published as: JPWO2022209074A1

Abstract

This analysis system is provided with a liquid chromatograph for separating a liquid sample into components and performing analysis thereof, a fraction collector that fractionates the liquid sample having passed through the liquid chromatograph and that drops a fractionated liquid having undergone fractionation to a sample holding body having a plurality of sample holding parts; an analysis device that analyzes the sample components contained in the fractionated liquid, which had been dropped onto the sample holding parts; and a setting screen display part for displaying, on a display, a drop range setting screen for setting a range of sample holding parts, which are among the plurality of sample holding parts and onto which a fractionated liquid is to be dropped by the fraction collector. The setting screen display part simultaneously displays, on the drop range setting screen, a setting chromatogram for setting a fractionation range of the liquid sample and a sample holding body symbol having a plurality of sample holding part symbols mimicking the sample holding parts, displays the fractionation range of the liquid sample on the setting chromatogram, and displays, on the sample holding body symbol, the sample holding part symbols corresponding to the fractionation range.

Description

Analysis system, analysis method and program for analysis system

The present invention relates to an analysis system, analysis method, and analysis system program using liquid chromatography.

As an analysis system of this type, for example, as shown in Patent Document 1, a liquid sample is separated and analyzed by an apparatus (liquid chromatograph) using the principle of liquid chromatography, and the liquid sample is separated using a fraction collector. There is also a method in which liquid samples are taken and successively dropped into a plurality of wells or the like provided on a plate, and the dropped liquid samples are analyzed by an analyzer using another principle such as Raman spectroscopy.

By the way, when setting the operation of the fraction collector in this type of analysis system, it is necessary to set the range from where to where in the liquid sample separated by the liquid chromatograph and to which well in the plate to drop it. There is a need to. Conventionally, when performing such operation settings, it was difficult to understand the correspondence between the liquid sample collection range and the drop position on the plate. It has to be done and it is not easy to use.

WO2014/027652

Therefore, the main object of the present invention is to provide a user-friendly analysis system in which it is easy to grasp the correspondence between the range of fractionation of the liquid sample by the fraction collector and the dropping position.

That is, the analysis system according to the present invention includes a liquid chromatograph that separates and analyzes a liquid sample for each component, separates the liquid sample that has passed through the liquid chromatograph, and divides the separated liquid into a plurality of samples. A fraction collector that drips onto a sample holder having a holder, an analyzer that analyzes a sample component contained in the fractionated liquid dripped onto each of the sample holders, and the fraction collector among the plurality of sample holders. a setting screen display unit for displaying, on a display, a dropping range setting screen for setting the range of the sample holding unit to which the fractionated liquid is dropped, wherein the setting screen display unit displays the sorting range of the liquid sample; A setting chromatogram for setting and a sample holder symbol having a plurality of sample holder symbols simulating the sample holder are simultaneously displayed on the dropping range setting screen, and on the setting chromatogram A collection range of the liquid sample is displayed, and the sample holder symbol corresponding to the collection range is displayed on the sample holder symbol.

With such a device, the setting chromatogram of the liquid sample and the sample holder symbol simulating the sample holder are displayed simultaneously on one screen, and the preparative collection range of the liquid sample is displayed on the setting chromatogram. In addition, since the sample holder symbol corresponding to this fractionation range is displayed on the sample holder symbol, the user can easily determine the fractionation range of the liquid sample and the position of the sample holder onto which the fractionated liquid is dropped. It is possible to visually comprehend the correspondence relationship between , and to set the operation of the fraction collector more easily than before.

In the analysis system, when the user selects the fractionation range of the liquid sample on the setting chromatogram, the sample holder symbol corresponding to the selected fractionation range is displayed on the sample holder symbol. , is displayed so as to be distinguishable from other sample holder symbols that do not correspond to the selected preparative collection range, and when the user selects the sample holder symbol on the sample holder symbol, the selected It is preferable that the preparative collection range corresponding to the sample holder symbol is displayed on the setting chromatogram.
In this way, it is possible to grasp how much the sample holder is used for the fractionation range of the selected liquid sample. Furthermore, it is possible to grasp which range of the liquid sample is dropped onto the selected sample holder.

The analysis system further includes a storage unit that stores drop start position information regarding the position of the sample holding unit where dropping of the preparative liquid starts, and dropping amount information concerning the amount of dropping onto each sample holding unit. and determining the sample holder onto which the preparative liquid is dropped based on the preparative collection range on the setting chromatogram selected by the user and the stored drop start position information and drop amount information. , the sample holder symbol on the sample holder symbol.
In this way, when setting the operation of the fraction collector, it is possible to grasp in more detail how much the sample holder is used.

Further, the analysis system displays an error message on the display when the number of the sample holders determined by the dropping range determination unit exceeds the number of the sample holder symbols that can be displayed in the sample holder symbol. is preferably displayed.
In this way, the user can understand that the set information on the drip start position and the drip amount is not appropriate, and usability can be further improved.

In addition, in the analysis system, the user moves a bar displayed on the setting chromatogram to select the fractionation range of the liquid sample, and in conjunction with the movement of the bar, It is preferable that the specimen holding portion symbol displayed is configured to change its form.
With this configuration, when the position of the bar on the screen is moved by a drag operation or the like, the aspect of the sample holder symbol on the sample holder symbol changes, so that the range of fractionation of the liquid sample and the amount of liquid to be fractionated change. It is possible to make it easier to visually grasp the corresponding relationship with the sample holding portion to which the sample is dropped.

The analysis system further includes an analysis result display unit for displaying an analysis result screen showing analysis results, and the analysis result display unit includes an analysis chromatogram, which is an analysis result of the liquid sample by the liquid chromatograph, and the sample. When the user selects the fractionation range on the analysis chromatogram, the sample holder symbol corresponding to the selected fractionation range is displayed on the sample holder symbol. , are displayed so as to be distinguishable from other sample holder symbols that do not correspond to the fractionation range, and when the user selects the sample holder symbol on the sample holder symbol, the symbol corresponding to the sample holder symbol is displayed. It is preferable that the fractionation range is displayed on the setting chromatogram.
In this way, when reviewing the analysis results, it is possible to easily grasp the correspondence relationship between the fractionation range in the chromatograph obtained by the analysis and the sample holder into which the fractionated liquid was dropped.

In this case, it is preferable that the analysis result display unit further displays the analysis result of the analysis device on the analysis result screen.

Further, in the analysis system, it is preferable that the analysis device analyzes by Raman spectroscopy.
In this way, the sample components contained in the fractionated liquid separated by the liquid chromatograph are analyzed by Raman spectroscopy. It is possible to detect extremely small amounts of components that cannot be detected by conventional methods.

A specific embodiment of the sample holder is preferably a plate having a plurality of wells formed as the sample holder.
In this way, it can be used more preferably in combination with an analysis device using Raman spectroscopy.

Further, the analysis method according to the present invention includes a liquid chromatograph for separating and analyzing a liquid sample for each component, fractionating the liquid sample that has passed through the liquid chromatograph, An analysis method using a fraction collector that drips onto a sample holder having a sample holder, and an analyzer that analyzes sample components contained in the fractionated liquid dropped onto each of the sample holders, wherein the plurality of Displaying on the display a dropping range setting screen for setting the range of the sample holding part to which the fraction collection liquid is dropped by the fraction collector among the sample holding parts of No., and for setting the fractionation range of the liquid sample A setting chromatogram and a sample holder symbol having a plurality of sample holder symbols simulating the sample holder are simultaneously displayed on the dropping range setting screen, and fractionation of the liquid sample is performed on the setting chromatogram. The range is displayed, and the sample holder symbol corresponding to the preparative collection range is displayed on the sample holder symbol.

Further, the analysis system program according to the present invention includes a liquid chromatograph for separating and analyzing a liquid sample for each component, fractionating the liquid sample that has passed through the liquid chromatograph, and A program used in an analysis system comprising: a fraction collector that drips onto a sample holder having a plurality of sample holders; a setting screen display unit for displaying on a display a dropping range setting screen for setting a range of the sample holding part to which the fraction collector drops the fractionated liquid among the plurality of sample holding parts. A setting chromatogram for setting the fractionation range of the liquid sample and a sample holder symbol having a plurality of sample holder symbols simulating the sample holder are displayed on the computer, and the setting screen display unit displays the is simultaneously displayed on the dropping range setting screen, the fractionation range of the liquid sample is displayed on the setting chromatogram, and the sample holder symbol corresponding to the fractionation range is displayed on the sample holder symbol. It is characterized by displaying.

According to such an analysis method and analysis system program, the same effects as those of the analysis system described above can be achieved.

As described above, according to the present invention, it is possible to provide an analysis system that is easy to use because it is easy to grasp the correspondence between the range of fractionation of the liquid sample by the fraction collector and the dropping position.

1 is a schematic diagram showing the overall configuration of an analysis system in one embodiment of the present invention; FIG. The functional block diagram which shows the function of the integrated management apparatus in the same embodiment. The figure which shows the one aspect|mode of the drip range setting screen in the same embodiment. The figure which shows the one aspect|mode of the analysis result screen in the same embodiment.

100... Analysis system S... Liquid sample SS... Preparative liquid PL... Plate 10... Chromatograph 20... Fraction collector 30... Raman spectroscopic analyzer 50... Drop range Setting screen 51: Setting chromatogram S _PL : Plate symbol S _W : Well symbol

An analysis system according to one embodiment of the present invention will be described below with reference to the drawings.

The analysis system 100 of this embodiment performs LC-Raman analysis using both liquid chromatography and Raman spectroscopy, and is a type of so-called hyphenated technology.

Specifically, as shown in FIG. 1, this analysis system includes a liquid chromatograph 10, a fraction collector 20, and a Raman spectroscopic analysis device 30, and integrated management of setting of these operations, data analysis, display of analysis results, etc. and an integrated management device 40 that The liquid chromatograph 10, the fraction collector 20, and the Raman spectroscopic analysis device 30 are equipped with

control calculators

1C, 2C, and 3C, which are dedicated software for performing respective hardware operations and data analysis. The integrated management device 40 is configured to operate as overlay software, and the integrated management device 40 and the

control calculators

1C, 2C, and 3C of each device cooperate to operate integrally as one analysis system 100. do.

I will explain each device in detail.

The liquid chromatograph 10 separates and detects each component of the liquid sample S by liquid chromatography.

Specifically, the liquid chromatograph 10, as shown in FIG. By feeding the liquid sample S together with the phase Z to the separation column 14, the liquid sample S is separated for each component. A component detector 15 for detecting separated components of the liquid sample S is provided downstream of the separation column 14 .

The mobile phase Z is, for example, a mixed liquid in which a plurality of types of liquids are mixed, and here is a mixed liquid of water and an organic solvent such as ethanol. However, the mobile phase Z may consist of a single liquid, or may be a gradient solvent having a concentration gradient.

The liquid chromatograph 10 further includes an LC control calculator 1C that controls each device such as the pump P and generates a chromatogram of the liquid sample S based on the output of the component detector 15. The LC control arithmetic unit 1C is composed of a dedicated or general-purpose computer, executes a chromatographic program stored in a memory, and realizes its functions through the cooperation of each device.

The fraction collector 20 is provided on the downstream side of the component detector 15 of the liquid chromatograph 10 as shown in FIG. In this embodiment, the fraction collector 20 is mounted on a plate PL (sample holder in the claims) in which a plurality of wells (sample holders in the claims) W are formed in a matrix. On the other hand, it is configured such that a plurality of sample components separated from the liquid sample S are dropped into different wells W, respectively. In the following, the liquid fractionated on the plate PL by the fraction collector 20 is referred to as a fractional liquid SS, and the fractional liquid SS is not limited to a mobile phase Z containing components derived from the liquid sample S. , the concept includes those consisting only of the mobile phase Z.

The configuration of the fraction collector 20 will be described in detail. It is configured to drop SS in predetermined amounts one after another.

The fraction collector 20 also includes a stacker 24 that stores a plurality of plates PL. This stacker 24 is for waiting the plate PL before the separation liquid SS is dropped into the well W and the plate PL until the separation liquid SS is dropped into the well W and dried (dried). is. A maximum of three plates PL can be placed on each of the three racks A, B, and C of the stacker 24 . Movement of the plate PL between the stacker 24 and the stage 22 is performed by a transport mechanism (not shown).

In addition, the fraction collector 20 is further provided with a fraction control calculator 2C that controls the mobile probe 21 and the like and generates fraction information, which is information about the state of dropping into each well W of each plate PL. The fraction control arithmetic unit 2C is composed of a dedicated or general-purpose computer, executes a chromatographic program stored in a memory, and realizes its functions through the cooperation of each device.

More specifically, as shown in FIG. 2, the fraction control computing unit 2C determines the position of the mobile probe 21 and the fractionation conditions (fractionation flow rate, fraction It has at least a fraction control section 2C1 for controlling the time taken, etc.). In addition, the fraction control computing unit 2C, based on the fraction setting information input from the integrated management device 40, predicts the fraction result, which is the usage range of the wells W in the plate PL when the preparative liquid SS is dropped, and the plate A fraction information generation unit 2C2 is further provided for generating fraction information such as a fraction result when the preparative liquid SS is actually dripped onto the PL. Data relating to the fraction information generated by the fraction information generating section 2C2 is transmitted to the integrated management device 40. FIG. The integrated management device 40 generates and stores a correspondence relationship between the position of the well W into which the preparative liquid SS is dropped and the area on the chromatogram.

Next, the Raman spectroscopic analysis device 30 will be explained. The Raman spectroscopic analyzer 30 analyzes the sample components contained in the sampled liquid SS dropped in the wells W on the plate PL in a dried state based on Raman spectroscopy. As for the plate PL, the user carries it to the Raman spectroscopic analyzer 30 after it has been dried in the fraction collector 20. A plate PL may be transported.

As shown in FIG. 1, the Raman spectroscopic analysis apparatus 30 includes a light irradiator 31 that irradiates excitation light such as laser light to the wells W on the plate PL holding the preparative liquid SS, and a light irradiator 31 that irradiates the excitation light. A spectroscope 32 for spectroscopy the Raman scattered light generated from the sample components contained in the preparative liquid SS, a Raman scattered light detector 33 for detecting the spectroscopic Raman scattered light, and wells W irradiated with light. and a camera 34 for taking micrographs.

In addition, the Raman spectroscopic analyzer 30 controls the position on the plate PL where the laser light emitted by the light irradiator 31 is irradiated, and Raman control for generating a Raman spectrum based on the output of the Raman scattered light detector 33. A calculator 3C is further provided. The Raman control computing unit 3C is composed of a dedicated or general-purpose computer, executes a program dedicated to the Raman spectroscopic analysis apparatus stored in memory, and realizes its functions through the cooperation of each device.

Next, the integrated management device 40 will be explained. The integrated management device 40 is connected to the LC control computing unit 1C, the fraction control computing unit 2C, and the Raman control computing unit 3C via a wired or wireless network, and has various functions between the

control computing units

1C, 2C, and 3C. It transmits and receives information, and is specifically composed of a dedicated or general-purpose computer equipped with a CPU, memory, and the like. The integrated management device 40 transmits setting information (chromatograph setting information, fraction setting information, Raman spectroscopic analysis setting information) for setting parameters related to analysis to each of the

control calculators

1C, 2C, and 3C, It also receives information on analysis results obtained from the

respective control calculators

1C, 2C, and 3C, results of operations performed on the plate PL, and the like.

The integrated management device 40 cooperates with the CPU and other peripheral devices in accordance with the analysis system program stored in the memory, thereby providing an input receiving section 41, a setting screen display section, and a setting screen display section as shown in FIG. 42 , setting information generation unit 43 , storage unit 44 and analysis result display unit 45 at least.

The input reception unit 41 receives various inputs from the user through input devices such as keyboards and mice.

The setting screen display unit 42 displays a setting screen regarding each of the

devices

10, 20, and 30 on the display DP according to the input from the user. For example, the setting screen display unit 42 displays a plate selection screen (not shown) for setting which plate PL set in the stacker 23 is to be used for sorting the liquid sample S, and a plate selection screen (not shown) shown in FIG. A dropping range setting screen 50 or the like for setting the dropping range of the fraction collector 20 (that is, the range in which the sorting liquid SS is dropped among the plurality of wells W on the plate PL) is displayed on the display DP. to display. The user can set the

devices

10, 20, and 30 by inputting information on various setting screens displayed on the display DP.

The setting information generation unit 43 generates chromatograph setting information, fraction setting information, and Raman spectroscopic analysis setting information related to analysis based on the user's input to the setting screen, and transmits them to each of the

devices

10, 20, and 30. It is.

For example, when the user inputs necessary information on the dripping range setting screen 50 and approves the dripping setting, the setting information generating unit 43 generates fraction setting information such as fractionation parameters to be transmitted to the fraction collector 20. . Then, the generated fraction setting information is transmitted from the integrated management device 40 to the fraction control computing unit 2C, and each device of the fraction collector 20 is controlled by the fraction control unit 2C1 to dispense the sample components onto the actual plate PL. is done.

In addition, the setting information generation unit 43 generates setting information (chromatograph setting information) of the liquid chromatograph 10 and Raman spectroscopic analysis based on the user's input to the setting screen of the liquid chromatograph 10 and the setting screen of the Raman spectroscopic analysis device 30 . Setting information (Raman spectroscopic analysis setting information) of the device 30 is generated and transmitted to the

corresponding devices

10 and 30 .

The storage unit 44 is set in a predetermined area of the memory, and stores various information input by the user and various setting information (chromatograph setting information, fraction setting information, Raman spectroscopy information) generated by the setting information generation unit 43. analysis setting information), information on analysis results and operation results received from each

device

10, 20, 30, information on the plate PL being used, and the like. This operation result includes fraction information such as a fraction result.

The analysis result display unit 45 displays an integrated analysis result screen 60 on the display DP based on the information recorded in the storage unit 44 . Specifically, the analysis result display unit 45 refers to the analysis results and operation results received from the

devices

10, 20, and 30 recorded in the storage unit 44, and generates, for example, an analysis result screen 60 shown in FIG. and displayed on the display DP. On this analysis result screen 60, at least the chromatogram (analysis chromatogram) of the liquid sample, which is the analysis result of the liquid chromatograph 10, and the Raman spectrum of the sample component, which is the analysis result of the Raman spectrometer 30, are displayed side by side. be done.

As described above, in the analysis system 100 of the present embodiment, it is possible to set each

device

10, 20, 30 necessary for starting analysis only by inputting to the integrated management device 40, and each

device

10, 20, 30 A series of analyzes can be performed without operating dedicated software, and the analysis results can be confirmed.

Therefore, in the analysis system 100 of the present embodiment, when setting the operation of the fraction collector 20, it is possible to easily grasp the correspondence relationship between the fractionation range of the liquid sample S and the well W into which the fractionation liquid SS is dropped. In order to do so, the setting screen display unit 42 displays a setting chromatogram 51 for setting the preparative collection range of the liquid sample S and a plate symbol S _PL imitating the plate PL (as the sample holder symbol in the claims). equivalent) are displayed side by side on the dropping range setting screen 50 at the same time, and the separation range of the liquid sample S is displayed on this setting chromatogram 51, and the separation liquid SS is dropped corresponding to this separation range. A well symbol S _W (corresponding to a sample holder symbol in the scope of claims) that imitates the well W in which the plate is located is displayed on the plate symbol S _PL .

Specifically, the setting chromatogram 51 is based on, for example, a chromatogram that is an analysis result of another liquid sample having similar sample components to the liquid sample S to be analyzed, or a past analysis result of the liquid chromatograph 10. It is a created model or the like, which is input in advance by the user and stored in the storage unit 44 . Two bars 52 are displayed on the setting chromatogram 51 to indicate the fractionation range of the liquid sample S (also referred to as the fraction period). The area between the two bars 52 indicates the sorting range. The user can select the fractionation range by moving the positions of the two bars 52 on the setting chromatogram 51 by a drag operation or the like.

The plate symbol S _PL is a schematic plan view of the plate PL to be used. On the plate symbol SPL, a plurality of well symbols SW imitating a plurality of wells _W are displayed in a matrix so as to have the same number and arrangement as the actual plate _PL . On the plate symbol SPL, a predicted dropping range of the sample liquid SS is displayed according to the sample collection range displayed on the setting chromatogram _51. FIG. Specifically, as shown in FIG. 3, among a plurality of well symbols _SW , the mode (color, size, brightness, etc.) of the well symbol _SW predicted to drop the sample liquid SS is changed. The drop range on the plate PL is indicated by .

In such a dropping range setting screen 50, when the user selects the fractionation range of the liquid sample S on the setting chromatogram 51, the mode of each well symbol _SW on the plate symbol _SPL is changed accordingly. The well symbol _SW corresponding to the selected fractionation range is displayed so as to be distinguishable from other well symbols _Sw . Conversely, when the user selects one or a plurality of well symbols _SW on the plate symbol SPL, the position of the bar 52 moves accordingly on the setting chromatogram 51, and the selected well symbol _S The fractional collection range of the liquid sample S corresponding to _W is displayed.

Further, on this dropping range setting screen 50, information (dropping start position information) on the position of the well W where dropping of the preparative liquid SS is started, and the amount (or dropping time) of dropping from the mobile probe 21 to each well W are displayed. Input boxes B1 and B2 are provided for inputting information (dripping amount information) regarding. When the user inputs the dropping start position and the dropping amount into the input boxes B1 and B2 and changes the settings, the dropping range displayed on the plate symbol _SPL is changed in consideration of these information. That is, in this embodiment, the well W to which the preparative liquid SS is dropped is determined based on the preparative collection range on the setting chromatogram 51 selected by the user and the input dropping start position information and dropping amount information. A range (number) is determined and configured to be reflected on the plate symbol S _PL . If the determined number of wells W exceeds the number that can be displayed on the plate symbol _SPL , the dropping range setting screen 50 is configured to display an error message. Then, when the setting of the dripping range is confirmed by the user's operation such as clicking the confirm button B3 displayed on the dripping range setting screen 50, information on the setting of the dripping range (dripping position, dripping amount, drop start position, start time, etc.) are stored in the storage unit 44 .

In addition, in the analysis system 100 of the present embodiment, when confirming the analysis result, the analysis is performed in order to easily grasp the correspondence relationship between the fractionation range of the liquid sample S and the well W into which the fractionation liquid SS was dropped. The result display unit 45 refers to the information about the dropping position stored in the storage unit 44, and the analysis chromatogram 61, which is the analysis result of the liquid sample S by the liquid chromatograph 10, and the plate symbol S simulating the plate PL. _PL and PL are simultaneously displayed on the analysis result screen 60, the fractionation range of the liquid sample S is displayed on this analysis chromatogram 61, and the well W into which the fractionation liquid SS is dropped corresponding to this fractionation range is displayed. It is configured to be displayed on the plate symbol _SPL . Here, the plate symbol S _PL displayed on the analysis result screen 60 has the same aspect as the plate symbol S _PL displayed on the dropping range setting screen 50 .

In this analysis result screen 60, when the user selects an arbitrary preparative range on the analysis chromatogram 61, the aspect (for example, color) of each well symbol _SW on the plate symbol _SPL changes accordingly. The well symbol _SW corresponding to the selected preparative collection range is displayed so as to be distinguishable from other well symbols _SW . Conversely, when the user selects one or more well symbols S _W on the plate symbol S _PL (eg, the well symbol S _W for C1 in FIG. 4), a corresponding bar is displayed on the analytical chromatogram. 62 is displayed, and the collection range corresponding to the selected well symbol _SW is displayed.

The analysis result of the Raman spectroscopic analyzer 30 is also displayed on the analysis result screen 60 at the same time. Specifically, when one well symbol _SW is selected by the user from the plate symbol _SPL , the Raman spectrum and the microscopic image of the sample liquid SS dropped in the well corresponding to the well symbol _SW are displayed. An image 64 is displayed.

According to the analysis system 100 of the present embodiment configured as described above, the setting chromatogram 51 of the liquid sample S and the plate symbol S _PL are simultaneously displayed on the dropping range setting screen 50, and the liquid sample S is displayed on the setting chromatogram 51. The fractionation range of the sample S is displayed, and the well W from which the fractionation liquid SS corresponding to this fractionation range is fractionated is displayed on the plate symbol _SPL . The relationship between the range of fractionation and the position of the well W into which the fractionation liquid SS is dropped can be visually grasped easily, and the operation setting of the fraction collector 20 can be performed more easily than before.

Further, when the user selects the fractionation range of the liquid sample S on the setting chromatogram 51, the well symbol SW corresponding to the selected fractionation range is changed to another well symbol _S on the plate symbol _SPL . When the well symbol _SW is selected by the user on the plate symbol _SPL , the fractionation _range corresponding to the selected well symbol _SW is displayed on the setting chromatogram 51. Therefore, it is possible to grasp how many wells W are used for the selected fractionation range of the liquid sample S, and to what extent the liquid sample S is fractionated for the wells W on the plate. It can be grasped whether SS is dropped.

In addition, on the analysis result screen 60, the analysis chromatogram 61 of the liquid sample S and the plate symbol S _PL are displayed at the same time. Since the well W in which the preparative solution _SS corresponding to . It is possible to easily grasp the correspondence relationship with the well W into which the liquid SS is dropped.

The present invention is not limited to the above embodiments.

For example, in the above embodiment, the analysis chromatogram 61 and the plate symbol _SPL are displayed side by side on the analysis result screen 60, but the present invention is not limited to this. In other embodiments, the plate symbol S _PL may not be displayed on the analysis result screen 60 . By displaying the analysis chromatogram 61 and the plate symbol _SPL side by side at least on the dropping range setting screen 50, the effects of the present invention can be achieved.

In the above-described embodiment, the user moves the position of the bar 52 displayed on the setting chromatogram 51 on the dropping range setting screen 50 to set the collection range of the liquid sample S. Not exclusively. For example, the collection range may be set by inputting the start time and end time of the fraction as numerical values in the input boxes provided on the dropping range setting screen 50 .

Further, the analysis system 100 of the present invention only needs to be configured to separate the liquid sample S by liquid chromatography, and does not necessarily need to include the component detector 15 constituting the liquid chromatograph 10, for example. Further, the analysis system 100 of the present invention does not necessarily have to include the Raman spectroscopic analysis device 30, and the liquid sample S separated by liquid chromatography is analyzed by, for example, infrared spectroscopy, nuclear magnetic resonance, or time-of-flight mass spectroscopy. It may be provided with an analysis device that performs analysis using the principle of an analysis method or the like.

In addition, in the above-described embodiment, the preparative liquid SS is dropped into a plurality of wells W formed on the plate PL, but the present invention is not limited to this. In the analysis system 100 of the present invention, the fractionated liquid SS may be dropped onto a sample holder provided with a plurality of sample holders collectively. An example of such a sample holder is a test tube rack that uses an independent container such as a test tube as the sample holder, and that can accommodate a plurality of containers and these containers in a regularly arranged state. and the like can be exemplified. The sample holder in which a plurality of sample holders are integrated is not limited to a plate having wells as described above. A plate or the like may be used. Moreover, in the sample holder, each sample holder may not be integrally fixed.

Although there is no particular limit to the number of sample holders provided in one sample holder, the effect of the present invention can be more pronounced as the number increases, such as 50 or more, 100 or more, or 200 or more. can.

In addition, it goes without saying that the present invention is not limited to the above-described embodiments, and that various modifications are possible without departing from the spirit of the present invention.

According to the present invention, it is possible to provide a user-friendly analysis system in which it is easy to grasp the correspondence between the liquid sample collection range by the fraction collector and the dropping position.

Claims

A liquid chromatograph for separating and analyzing a liquid sample for each component,
a fraction collector that fractionates the liquid sample that has passed through the liquid chromatograph and drops the fractionated fractionated liquid onto a sample holder having a plurality of sample holders;
an analysis device that analyzes sample components contained in the fractionated liquid dropped onto each of the sample holding units;
a setting screen display unit for displaying, on a display, a dropping range setting screen for setting a range of the sample holding unit onto which the preparative liquid is dropped by the fraction collector among the plurality of sample holding units;
The setting screen display unit sets the dropping range by setting a setting chromatogram for setting the fractionation range of the liquid sample and a sample holder symbol having a plurality of sample holder symbols imitating the sample holder. An analysis system for simultaneously displaying on a screen, displaying the fractionation range of the liquid sample on the setting chromatogram, and displaying the sample holder symbol corresponding to the fractionation range on the sample holder symbol.
When the user selects the fractionation range of the liquid sample on the setting chromatogram, the sample holder symbol corresponding to the selected fractionation range is displayed on the sample holder symbol. displayed in a distinguishable manner from the other sample holder symbols that do not correspond to the preparative range,
2. The set-up chromatogram according to claim 1, wherein when the user selects the sample holder symbol on the sample holder symbol, the fractionation range corresponding to the selected sample holder symbol is displayed on the setting chromatogram. analysis system.
a storage unit that stores dropping start position information about the position of the sample holding unit where dropping of the preparative liquid starts and dropping amount information about the amount of dropping to each sample holding unit;
determining the sample holder to which the preparative liquid is dropped based on the preparative collection range on the setting chromatogram selected by the user and the stored drop start position information and drop amount information; 3. The analysis system of claim 2, configured to mirror the sample holder symbol on the sample holder symbol.
The analysis system according to claim 3, wherein an error message is displayed on the display when the determined number of the sample holders exceeds the number of the sample holder symbols that can be displayed on the sample holder symbol.
A preparative range of the liquid sample is selected by the user moving a bar displayed on the setting chromatogram,
5. The analysis system according to any one of claims 1 to 4, wherein a mode of said sample holder symbol displayed on said sample holder symbol is changed in conjunction with movement of said bar.
further comprising an analysis result display unit for displaying an analysis result screen showing analysis results,
the analysis result display unit simultaneously displays an analysis chromatogram, which is an analysis result of the liquid sample by the liquid chromatograph, and the sample holder symbol;
When the user selects the preparative collection range on the analysis chromatogram, the sample holder symbol corresponding to the selected preparative collection range is displayed on the sample holder symbol not corresponding to the preparative collection range. Displayed so as to be distinguishable from the other sample holder symbols,
6. The preparative collection range corresponding to the sample holder symbol is displayed on the setting chromatogram when the user selects the sample holder symbol on the sample holder symbol. The analysis system according to item 1.
The analysis system according to claim 6, wherein the analysis result display unit further displays the analysis result of the analysis device on the analysis result screen.
The analysis system according to any one of claims 1 to 7, wherein the analysis device analyzes by Raman spectroscopy.
The analysis system according to any one of claims 1 to 8, wherein the sample holder is a plate in which a plurality of wells are formed as the sample holder.
A liquid chromatograph that separates and analyzes each component of a liquid sample, and the liquid sample that has passed through the liquid chromatograph is fractionated, and the fractionated liquid is stored in a sample holder having a plurality of sample holders. An analysis method using a dropping fraction collector and an analyzer for analyzing a sample component contained in the fractionated liquid dropped onto each sample holder,
causing a display to display a dropping range setting screen for setting a range of the sample holding part onto which the preparative liquid is dropped by the fraction collector among the plurality of sample holding parts;
simultaneously displaying a setting chromatogram for setting the fractionation range of the liquid sample and a sample holder symbol having a plurality of sample holder symbols simulating the sample holder on the dropping range setting screen; An analysis method characterized by displaying a preparative range of the liquid sample on a setting chromatogram, and displaying the sample holder symbol corresponding to the preparatory range on the sample holder symbol.
A liquid chromatograph that separates and analyzes each component of a liquid sample, and the liquid sample that has passed through the liquid chromatograph is fractionated, and the fractionated liquid is stored in a sample holder having a plurality of sample holders. A program for use in an analysis system comprising a dripping fraction collector and an analyzer for analyzing sample components contained in the sample liquid dripped onto each sample holder,
The computer functions as a setting screen display unit for displaying on a display a dropping range setting screen for setting the range of the sample holding part to which the preparative liquid is dropped by the fraction collector among the plurality of sample holding parts. let
The setting screen display unit sets the dropping range by setting a setting chromatogram for setting the fractionation range of the liquid sample and a sample holder symbol having a plurality of sample holder symbols imitating the sample holder. are simultaneously displayed on the screen, the fractionation range of the liquid sample is displayed on the setting chromatogram, and the sample holder symbol corresponding to the fractionation range is displayed on the sample holder symbol. A program for an analysis system that