WO2022179146A1 - 一种以潮间带沉积物为接种体系的高盐废水纯膜mbbr启动方法 - Google Patents

一种以潮间带沉积物为接种体系的高盐废水纯膜mbbr启动方法 Download PDF

Info

Publication number
WO2022179146A1
WO2022179146A1 PCT/CN2021/126295 CN2021126295W WO2022179146A1 WO 2022179146 A1 WO2022179146 A1 WO 2022179146A1 CN 2021126295 W CN2021126295 W CN 2021126295W WO 2022179146 A1 WO2022179146 A1 WO 2022179146A1
Authority
WO
WIPO (PCT)
Prior art keywords
sub
mbbr
intertidal
sediments
film
Prior art date
Application number
PCT/CN2021/126295
Other languages
English (en)
French (fr)
Inventor
施雪卿
张海峰
毕学军
于童
黄浩勇
赵飞
张楠
Original Assignee
青岛理工大学
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 青岛理工大学 filed Critical 青岛理工大学
Publication of WO2022179146A1 publication Critical patent/WO2022179146A1/zh

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/02—Aerobic processes
    • C02F3/12—Activated sludge processes
    • C02F3/1205—Particular type of activated sludge processes
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2305/00—Use of specific compounds during water treatment
    • C02F2305/06—Nutrients for stimulating the growth of microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

Definitions

  • the invention belongs to the technical field of wastewater biochemical treatment, and in particular relates to a high-salt wastewater pure membrane MBBR startup method using intertidal sediments as an inoculation system, in particular, it can directly realize high-salt organic wastewater pure membrane under specific high salinity conditions MBBR handles the quick start of the system.
  • Moving Bed Biofilm Reactor is an innovative biofilm reactor.
  • the filler in the reactor moves freely under the action of the gyratory inversion of the mixed solution, which can promote a large number of microorganisms to accumulate on the surface of the filler.
  • MBBR Moving Bed Biofilm Reactor
  • the filler in the reactor moves freely under the action of the gyratory inversion of the mixed solution, which can promote a large number of microorganisms to accumulate on the surface of the filler.
  • it has irreplaceable advantages compared with the traditional activated sludge method.
  • How to realize the rapid film formation of specific functional microorganisms on the filler and realize the rapid startup of the MBBR system Maintaining the efficient and stable operation of the system is the key to the efficient treatment of high-salt wastewater by the MBBR process.
  • the start-up of high-salt biofilm process mainly revolves around the domestication of traditional activated sludge or the inoculation of halophilic bacteria.
  • traditional activated sludge is used to treat high-salt wastewater by salinity gradient acclimation. This method has a long start-up period, and the screened microorganisms suitable for high-salt wastewater have limited resistance to load and salinity shock.
  • CN101723539A discloses a method and application for the treatment or reuse of high-salt wastewater, mainly through the combined process of "pretreatment + high-efficiency aeration biological filter + advanced treatment" to treat high-salt wastewater, in which high-efficiency aeration
  • the gas biological filter was inoculated with the engineered bacteria provided by a company in the United States.
  • the method is suitable for the treatment and reuse of high-salt wastewater and the renovation and upgrading of existing projects.
  • it can only be realized by adding American engineered flora, and the application is limited.
  • the present invention provides a method for starting MBBR with high-salt wastewater pure membrane using intertidal zone sediments as an inoculation system.
  • the invention inoculates the intertidal zone sediment in the MBBR system, improves the overall salt tolerance level of the system, shortens the start-up period, maintains the efficient and stable operation of the system, and broadens the biological treatment category of high-salt wastewater.
  • Intertidal zone refers to the coast between the average highest tide level and the lowest tide level, that is, the area that is submerged when the sea rises to the highest level and emerges from the water surface when the tide recedes to the lowest level.
  • Intertidal zone sediment refers to the sediment excavated in the intertidal zone wetland, as the inoculum of the present invention, which contains a complex flora that can both efficiently degrade organic pollutants and have good salt tolerance.
  • VSS Volatile Suspended Solids, refers to the content of microorganisms.
  • TSS total suspended solids content.
  • VSS/TSS refers to the ratio of microbial content in intertidal sediments.
  • MLSS The concentration of suspended solids in the mixture.
  • a method for starting high-salt wastewater pure membrane MBBR using intertidal sediments as an inoculation system comprising the steps of inoculating the intertidal sediments into a high-salt wastewater pure membrane MBBR reactor, packing a film and forming a biofilm the domestication steps.
  • the salinity of the intertidal sediment is 1%-4%, preferably 2.5%-3.5%;
  • the VSS/TSS of the intertidal sediments is 0.08-0.18.
  • the intertidal zone sediment is taken from 0-5 cm of the surface layer of the intertidal wetland.
  • the surface of the intertidal sediments is yellowish brown or dark brown.
  • the intertidal sediments have a delicate texture and no obvious sandy texture, similar to soil.
  • the intertidal sediment after the intertidal sediment is retrieved, it needs to be pretreated before inoculation because it contains a large amount of inorganic particles;
  • the preprocessing steps are as follows:
  • Potassium control the influent COD to 500mg/L, ammonia nitrogen to 25mg/L, phosphate concentration to 5mg/L, control the sludge concentration MLSS to 10000mg/L, as simulated wastewater, carry out three aeration; continue to observe the COD in the container In the concentration change during the reaction process, when the COD removal rate is greater than 50%, replace the new simulated wastewater, and the first aeration ends; repeat the above operation for the second aeration, when the COD removal rate is greater than 80%, again Replace the simulated wastewater with a new one, and the second aeration ends; repeat the above operation for the third aeration, and the third aeration ends when the VSS/TSS of the intertidal sediments in the container is 0.65-0.75.
  • pretreated intertidal sediments are added into the MBBR reactor.
  • the inoculation concentration of the intertidal sediments in the high-salt wastewater MBBR reactor is 80-120 mg/L, preferably 100 mg/L.
  • the described filler hanging film step is carried out as follows:
  • the filler in the high-salt wastewater MBBR reactor is completely immersed in the mixed liquid containing the intertidal sediments, and fully aerated to make the filler just fluidized, and the intermittent flow method is adopted to ensure the high-salt wastewater MBBR reactor. There is sufficient suspended biomass in it, which promotes the formation of biofilm, and then realizes the film hanging of the MBBR reactor of high-salt wastewater.
  • the major elements and trace elements are shown in Table 1:
  • the acclimation step of the biofilm is carried out as follows:
  • the intermittent flow is changed to a continuous flow, and the new wastewater is replaced, leaving only the filler and the biofilm attached to the inside of the filler.
  • the turbidity of the mixture can adjust the water inflow rate, accelerate the formation of biofilm and promote the optimization of the bacterial community structure.
  • a process parameter optimization step is also included;
  • the core operating parameters are optimized in combination with effluent quality, biofilm apparent changes and microscopic inspection results to shorten the system startup period and improve the efficiency of pollutant reduction;
  • a high-salt wastewater MBBR reactor comprising a biological reaction device, a fluidization device, a screen interception device, an aeration pump and a feed pump;
  • the biological reaction device is a cylindrical container for containing the MBBR filler, and the biological reaction device is provided with a water inlet and a water outlet;
  • the fluidization device includes a circular aeration plate covering the bottom of the biological reaction device and a vertical
  • the mechanical stirring device arranged in the biological reaction device, the fluidization device is convenient for uniform aeration and auxiliary fluidization;
  • the screen interception device is an embedded fence structure, which is arranged at the water outlet of the biological reaction device to prevent fillers block the water outlet;
  • the aeration pump is connected to the aeration plate through a pipeline, and the feed pump is connected to the water inlet through a pipeline.
  • the water inlet is located below the other side of the water outlet.
  • the biological reaction device is a transparent cylindrical container, and the aspect ratio is (16-18):(48-52), more preferably 17:50.
  • the filler is made of polyethylene, polypropylene, polyurethane or materials modified with enzymatic agents and hydrophilic groups;
  • the shape of the filler is a hollow cylinder, including an inner, middle and outer three-layer structure. Vertical edges extend in different directions from the second layer in the middle; the outermost layer is circular, connected to the second layer by 12 concentric circles, and its cross section is formed by connecting end to end with multiple arcs.
  • the operating parameters of the high-salt wastewater MBBR reactor are:
  • Filler filling rate is 25%-45%, preferably 35%
  • the dissolved oxygen concentration is 2.0-7.0mg/L, preferably 3.0-5.0mg/L;
  • pH is 6.0-9.0, preferably 7.0-7.5;
  • the water temperature is 15-25°C.
  • microorganisms can be accelerated to attach and form a film inside the biological filler, shorten the film forming period of the MBBR process, promote the optimization of the biofilm flora structure, improve the overall salt tolerance level of the system, and widen the MBBR.
  • the present invention inoculates sediments in the intertidal zone, which can directly realize the rapid start-up of the MBBR system under the condition of salinity 1-4%, and maintain the efficient and stable operation of the system, which can effectively avoid the traditional activated sludge in the inoculation process. Serious biomass loss and large fluctuations in effluent quality;
  • the present invention inoculates intertidal zone sediments to carry out the film hanging of MBBR fillers, the steps are simple, the operation is convenient, and the preparation of salt-tolerant microbial inoculants can be skipped, which is convenient for industrialization and application;
  • intertidal sediments Compared with the salt-tolerant microbial inoculants with a single bacterial structure, the intertidal sediments have higher bacterial diversity and species richness (as shown in Table 2), and can adapt to a variety of complex compositions. High-salt organic wastewater; compared with traditional activated sludge, intertidal sediments have some unique, salt-tolerant functional microorganisms.
  • Example 1 is a schematic diagram of the main structure of the MBBR reactor for high-salt wastewater in Example 1 of the present invention, that is, a schematic diagram of an MBBR device.
  • Figure 2 is a photograph of blank filler (A) and film-hanging filler (B) in the high-salt wastewater MBBR reactor in Example 1 of the present invention, that is, a comparison diagram of the filler before and after film-hanging.
  • Example 3 is a treatment effect diagram of Example 1 of the present invention, that is, a treatment efficiency diagram of the MBBR system inoculated with intertidal sediments during the start-up period.
  • FIG. 4 is a treatment effect diagram of the comparative example of the present invention, that is, a treatment efficiency diagram of the MBBR system inoculated with activated sludge during the start-up period.
  • the intertidal zone sediment collection site adopted in the embodiment is the coastline of Huangdao District, Qingdao City, the intertidal zone sediment is taken from the intertidal wetland surface layer 0-5cm, and the intertidal zone sediment surface is yellowish brown or dark brown, This kind of intertidal sediment has a delicate texture, no obvious sandy texture, and is similar to soil.
  • the salinity of the intertidal sediment is 3%, and the VSS/TSS of the intertidal sediment is 0.24-0.34.
  • Table 2 The results of microbial community diversity analysis in intertidal sediments and activated sludge are shown in Table 2.
  • a high-salt wastewater MBBR reactor includes a biological reaction device 1, a fluidization device, a screen interception device 2, an aeration pump 3 and a feed pump 4;
  • the biological reaction device 1 is a transparent cylindrical container for containing the MBBR filler 5, and the biological reaction device 1 is provided with a water inlet 8 and a water outlet 9;
  • the screen interception device 2 is an in-line fence structure. At the water outlet 9 of the biological reaction device 1, prevent the filler 5 from blocking the water outlet 9;
  • the aeration pump 3 is connected to the aeration plate 7 through a pipeline, and the feed pump 4 is connected to the water inlet 8 through a pipeline;
  • the water inlet 8 is located below the water outlet 9, and the height-diameter ratio of the biological reaction device 1 is 17:50;
  • the filler 5 is made of polyethylene.
  • the shape of the filler 5 is a hollow cylinder, including an inner, middle and outer three-layer structure.
  • the inner layer is a regular hexagon.
  • the two layers are connected by concentric circles, and the inner layer and the middle second layer extend vertical edges in different directions; the outermost layer is circular, connected with the second layer concentric circles by 12 tails, and its cross-section is composed of multiple The arcs are connected end to end.
  • the operating parameters of the high-salt wastewater MBBR reactor are:
  • Filler filling rate is 25%-45%, preferably 35%
  • the dissolved oxygen concentration is 2.0-7.0mg/L, preferably 3.0-5.0mg/L;
  • pH is 6.0-9.0, preferably 7.0-7.5;
  • the water temperature is 15-25°C.
  • the height-diameter ratio of the biological reaction device 1 is 16:48, and the filler 5 is made of polypropylene.
  • the height-diameter ratio of the biological reaction device 1 is 18:52, and the filler 5 is made of polyurethane.
  • Embodiment 4 is a diagrammatic representation of Embodiment 4:
  • a method for starting high-salt wastewater pure-film MBBR using intertidal sediments as an inoculation system comprising using the high-salt wastewater MBBR reactor described in Embodiment 1, comprising: carrying out a tidal flow into the high-salt wastewater pure-film MBBR reactor The inoculation step of the interstitial sediments, the filler film hanging step and the acclimation step of the biofilm;
  • intertidal sediments After the intertidal sediments are retrieved, they need to be pretreated before inoculation because they contain a large number of inorganic particles.
  • Biofilm domestication When the COD removal rate in the system is greater than 80%, change the intermittent flow to continuous flow, replace the new wastewater, and leave only the filler (including the biofilm attached to the inside of the filler). Liquid turbidity (free microbial cell density) to adjust the water inflow rate. Gradually increase the organic load, accelerate the formation of biofilms and promote the optimization of the flora structure.
  • the salinity of the MBBR reactor inlet water is 3%
  • the dissolved oxygen concentration is 3.0-6.0 mg/L
  • the pH is 7.0-8.0
  • the water temperature is 20-25°C
  • the filler filling rate is 30%.
  • the high-salt wastewater is treated according to the method of Example 4, and the treatment effect diagram is shown in FIG. 3 .
  • the SCOD removal rate can reach 84% and the ammonia nitrogen removal rate can reach 99% during the start-up period of the MBBR process for treating high-salt wastewater.
  • the core parameters of the system are optimized, and the TOC of the influent water is adjusted to 650mg/L and the concentration of ammonia nitrogen is 100mg/L.
  • the TOC and ammonia nitrogen removal rates of the MBBR system can reach 95% and 98%, respectively.
  • the influent COD concentration is 500mg/L
  • the ammonia nitrogen concentration is 25mg/L
  • the phosphate concentration is 5mg/L.
  • the core operating parameters are optimized based on the water quality of the effluent, the apparent changes of the biofilm and the results of microscopic examination, and the organic load is controlled to 2.0kgCOD/(m 3.d), hydraulic pressure
  • the residence time is 12-24h
  • the dissolved oxygen concentration is 3.0-6.0mg/L
  • the pH value is 7.0-7.5.
  • the TOC removal rate of the MBBR system was 84.2%; the ammonia nitrogen removal rate was 57%, and the treatment effect was shown in Figure 4. It can be seen that the overall effect is significantly lower than the MBBR system inoculated with intertidal sediments.

Abstract

本发明涉及一种以潮间带沉积物为接种体系的高盐废水纯膜MBBR启动方法，包括：向高盐废水纯膜MBBR反应器内进行潮间带沉积物的接种步骤、填料挂膜步骤和生物膜的驯化步骤。采用本发明方法，通过接种潮间带沉积物，可以加速微生物在生物填料内部附着并成膜，缩短MBBR工艺的挂膜周期，促进生物膜菌群结构优化，提高系统整体耐盐水平，拓宽MBBR工艺在高盐废水中的应用范畴。本发明接种潮间带沉积物，可直接在盐度1？4％条件下实现MBBR系统的快速启动，并维持系统的高效稳定运行，可有效的避免传统活性污泥在接种过程中，生物量流失严重，出水水质波动大等问题。

Description

一种以潮间带沉积物为接种体系的高盐废水纯膜MBBR启动方法 技术领域
本发明属于废水生化处理技术领域，具体涉及一种以潮间带沉积物为接种体系的高盐废水纯膜MBBR启动方法，尤其是能直接在特定高盐度条件下实现高盐有机废水纯膜MBBR处理系统的快速启动。
背景技术
20世纪以来，在经济社会不断发展的背景下，化工、制药、食品加工、采油等工业活动快速发展，产生了大量的处理难度极大的高盐废水，其排放量约占世界污水排放量的5％。这些高盐废水中有毒有害，难降解有机物的含量较多，若未经处理直接排放，势必会对自然水体环境，生活饮用水等造成极大的破坏。物化法因其较高的能耗和二次污染等问题而不及生化法。然而废水中难降解有机物和过高的无机盐的存在对微生物的生长代谢产生明显的抑制作用，特别是在高盐条件下会导致微生物细胞原生质分离，代谢酶活性受阻，并直接影响活性污泥的凝聚沉降性能，使得传统生物处理工艺的应用受到限制。此外，在高盐和难降解碳源的双重压力胁迫环境下，微生物通常会启动降低代谢与增殖速率的自我保护机制。在这种条件下，如何在生化工艺运行过程中避免细菌生物量的流失，是维持生化系统高效运行和系统处理效率稳定性的重要保障。
移动床生物膜反应器(Moving Bed Biofilm Reactor，MBBR)是一种革新型生物膜反应器，该反应器内填料在混合液的回旋翻转作用下自由移动，能够促进微生物在填料表面大量富集，从而实现优势功能菌属的有效固定，并发挥其降解能力，相较于传统活性污泥法具有不可替代的优势，而如何实现特定功能微生物在填料上快速成膜，实现MBBR系统的快速启动，维持系统的高效稳定运行，是MBBR工艺高效处理高盐废水的关键所在。
目前，对于高盐生物膜工艺的启动主要是围绕传统活性污泥的驯化或是接种嗜盐菌两方面展开。通常采用盐度梯度驯化传统活性污泥处理高盐废水，这种方法启动周期较长，筛选出的适宜高盐废水的微生物，抗负荷、盐度冲击能力有限。而采用接种嗜盐菌的方法，存在着嗜盐菌的分离，培养操作繁琐，条件严苛，普通单位和个人难以实现，并且接种的嗜盐菌的生物构成比较简单，难以适应成分复杂的高盐有机废水等问题，例如：根据中国专利文件CN105152314A公开了一种高盐废水MBBR处理系统中悬浮填料的挂膜方法，该方法通过在MBBR系统中接种2.0-6.0g/L的耐盐微生物菌剂，控制体系内溶解氧2.0-6.0mg/L、pH＝6.0-9.0、水温15-45℃，采用同步培养或 异步培养的方式直接在特定盐度下实现MBBR系统悬浮填料的挂膜，可以有效的缩短挂膜周期，同时一定程度上也可以提高微生物对盐度的耐受范围；但该方法需参照现有技术中耐盐微生物菌剂制备方法，预先分离筛选出适盐菌属，一定程度上增加了运行成本和操作难度。再比如，CN101723539A公开了一种高含盐废水处理或回用的方法及用途，主要是通过“预处理+高效曝气生物滤池+深度处理”的组合工艺来处理高盐废水，其中高效曝气生物滤池中接种美国某公司提供的工程菌群。该方法适用于高含盐废水处理回用以及现有工程改造升级。但是，需要投加美国的工程菌群才可以实现，应用受到一定限制。
因此，急需一种操作简单、挂膜迅速、应用面广、盐度耐受范围高的MBBR系统启动方法。
发明内容
针对现有技术的不足，本发明提供一种以潮间带沉积物为接种体系的高盐废水纯膜MBBR启动方法。本发明在MBBR系统中接种潮间带沉积物，提高系统的整体耐盐水平，缩短启动周期，维持系统的高效稳定运行，拓宽高盐废水的生物处理范畴。
术语说明：
潮间带：是指平均最高潮位和最低潮位间的海岸，即海水涨至最高时所淹没的地方开始至潮水退到最低时露出水面的范围。
潮间带沉积物：是指在潮间带湿地挖取的沉积物，作为本发明的接种体，其中包含既能高效降解有机污染物又具有良好的耐盐能力的复合菌群。
VSS：挥发性悬浮固体，指微生物的含量。
TSS：总悬浮固体含量。
VSS/TSS：是指潮间带沉积物中微生物含量的比例。
MLSS：混合液悬浮固体浓度。
本发明的技术方案如下：
一种以潮间带沉积物为接种体系的高盐废水纯膜MBBR启动方法，包括：向高盐废水纯膜MBBR反应器内进行潮间带沉积物的接种步骤、填料挂膜步骤和生物膜的驯化步骤。
根据本发明，优选的，所述的潮间带沉积物的盐度为1％-4％，优选2.5％-3.5％；
优选的，所述的潮间带沉积物的VSS/TSS＝0.08-0.18。
优选的，所述的潮间带沉积物取自潮间湿地表层0-5cm处。潮间带沉积物表面呈黄褐色或是深褐色，这种潮间带沉积物质感细腻，无明显的沙质感，与土壤相似。
根据本发明，潮间带沉积物取回后，因其中含有大量的无机颗粒，需在接种前进行预处理；
优选的，预处理步骤如下：
将潮间带沉积物置于容器中，用盐度＝3％的盐水浸润；按C：N：P＝100:5:1的比例(质量浓度比)添加乙酸钠、氯化铵和磷酸二氢钾，控制进水COD为500mg/L，氨氮为25mg/L，磷酸盐浓度为5mg/L，控制其污泥浓度MLSS为10000mg/L，作为模拟废水，进行三次曝气；持续观察容器中COD在反应过程中的浓度变化，当COD去除率大于50％时，更换新的模拟废水，第一次曝气结束；重复上述操作进行第二次曝气，当COD去除率大于80％时，再次更换新的模拟废水，第二次曝气结束；重复上述操作进行第三次曝气，当容器中潮间带沉积物的VSS/TSS为0.65-0.75时第三次曝气结束。
根据本发明，优选的，所述的潮间带沉积物的接种，在MBBR反应器内投加预处理后的潮间带沉积物。
根据本发明，优选的，所述的高盐废水MBBR反应器中潮间带沉积物的接种浓度80-120mg/L，优选100mg/L。
根据本发明，优选的，所述的填料挂膜步骤，按如下方法进行：
接种后，使高盐废水MBBR反应器内的填料完全浸没在含有潮间带沉积物的混合液中，充分曝气，使得填料刚好流化，采取间断流的方式，保证高盐废水MBBR反应器内具有充足的悬浮态生物量，促进生物膜的形成，进而实现高盐废水MBBR反应器的挂膜。
根据本发明，优选的，所述的填料挂膜步骤中对高盐废水MBBR反应器内添加营养及微生物生长所需的常量元素和微量元素；进一步优选的，所述的营养为模拟含盐废水，其中按C：N：P＝100:5:1的比例(质量浓度比)添加乙酸钠、氯化铵和磷酸二氢钾，控制进水COD为500-2000mg/L，氨氮为25-100mg/L，磷酸盐浓度为5-20mg/L。所述的常量元素和微量元素如表1所示：
表1 常量及微量元素
Figure PCTCN2021126295-appb-000001
根据本发明，优选的，所述的生物膜的驯化步骤，按如下方法进行：
填料挂膜后，待高盐废水MBBR反应器内COD去除率大于80％时，改间断流为连续流，更换新的废水，仅留下填料及填料内部附着的生物膜，依据有机物去除率和混合液浊度(游离微生物细胞密度)来调整进水速率，加速生物膜的形成并促进菌群结构优化。
根据本发明，优选的，生物膜的驯化步骤之后，还包括工艺参数优化步骤；
在MBBR连续流处理高盐废水的过程中，结合出水水质、生物膜表观变化及镜检结果优化核心运行参数以缩短系统启动期，并提高污染物削减效率；
所述的核心运行参数控制为：有机负荷2.0-4.0kgCOD/(m 3.d)、水力停留时间12-24h，溶解氧浓度3.0-6.0mg/L，pH＝7.0-7.5。
根据本发明，还提供一种高盐废水MBBR反应器，包括生物反应装置、流化装置、筛网拦截装置、曝气泵和进料泵；
所述的生物反应装置为圆柱容器，用于盛放MBBR填料，生物反应装置设置有进水口和出水口；所述的流化装置包括铺满生物反应装置底部的圆形曝气盘和竖直设置在生物反应装置内的机械搅拌装置，流化装置便于均匀曝气以及辅助流化；所述的筛网拦截装置为内嵌式的栅栏结构，设置在生物反应装置的出水口处，防止填料对出水口堵塞；
所述的曝气泵通过管道与曝气盘连接，所述的进料泵通过管道与进水口连接。
根据本发明，优选的，所述的进水口位于出水口另一侧下方。
根据本发明，优选的，所述的生物反应装置为透明圆柱容器，高径比为(16-18)：(48-52)，进一步优选17：50。
根据本发明，优选的，所述的填料采用聚乙烯、聚丙烯、聚氨酯或上述物质利用酶促剂、亲水基团进行改性的材质；
优选的，填料的形状为中空圆柱体，包括内中外三层结构，内层为正六边形，正六边形的每个角向外延伸一尾翘，与中间第二层同心圆相连，内层和中间第二层向不同方向延伸出一条条竖棱；最外层为圆形，与第二层同心圆由12条尾翘相连，且其横截面由多个圆弧首尾相连而成。
根据本发明，优选的，所述的高盐废水MBBR反应器的运行参数为：
填料填充率为25％-45％，优选35％；
溶解氧浓度为2.0-7.0mg/L，优选3.0-5.0mg/L；
pH为6.0-9.0，优选7.0-7.5；
水温为15-25℃。
采用本发明方法，通过接种潮间带沉积物，可以加速微生物在生物填料内部附着并成膜，缩短MBBR工艺的挂膜周期，促进生物膜菌群结构优化，提高系统整体耐盐水平，拓宽MBBR工艺在高盐废水中的应用范畴。
与现有技术相比，本发明的有益效果：
1、本发明接种潮间带沉积物，可直接在盐度1-4％条件下实现MBBR系统的快速启动，并维持系统的高效稳定运行，可有效的避免传统活性污泥在接种过程中，生物量流失严重，出水水质波动大等问题；
2、本发明接种潮间带沉积物进行MBBR填料的挂膜，步骤简单，便于操作，可以跳过耐盐微生物菌剂的制备工作，便于工业化推广应用；
3、本发明与菌群结构单一的耐盐微生物菌剂相比，潮间带沉积物具有更高的菌群多样性与物种丰富度(如表2所示)，能够适应多种成分复杂的高盐有机废水；与传统活性污泥相比，潮间带沉积物中具有一些独特的、耐盐的功能微生物。
附图说明：
图1为本发明实施例1中高盐废水MBBR反应器的主体结构示意图，即MBBR装置示意图。
图2为本发明实施例1中高盐废水MBBR反应器中空白填料(A)及挂膜填料(B)的照片，即填料挂膜前后对比图。
图3为本发明实施例1的处理效果图，即接种潮间带沉积物的MBBR系统启动期处理效能图。
图4为本发明对比例的处理效果图，即接种活性污泥的MBBR系统启动期处理效能图。
其中：1、生物反应装置，2、筛网拦截装置，3、曝气泵，4、进料泵，5、填料，6、机械搅拌装置，7、曝气盘，8、进水口，9、出水口。
具体实施方式：
下面通过实施例并结合附图对本发明做进一步说明，但不限于此。
实施例中采用的潮间带沉积物采集地点为青岛市黄岛区海岸线，潮间带沉积物取自潮间湿地表层0-5cm处，潮间带沉积物表面呈黄褐色或是深褐色，这种潮间带沉积物质感细腻，无明显的沙质感，与土壤相似。潮间带沉积物的盐度为3％，所述的潮间带沉积物的VSS/TSS＝0.24-0.34。潮间带沉积物、活性污泥微生物群落多样性分析结果如表2所示。
表2 潮间带沉积物、活性污泥微生物群落多样性分析
实施例1
如图1所示，一种高盐废水MBBR反应器，包括生物反应装置1、流化装置、筛网拦截装置2、曝气泵3和进料泵4；
所述的生物反应装置1为透明圆柱容器，用于盛放MBBR填料5，生物反应装置1设置有进水口8和出水口9；所述的流化装置包括铺满生物反应装置1底部的圆形曝气盘7和竖直设置在生物反应装置1内的机械搅拌装置6，流化装置便于均匀曝气以及辅助流化；所述的筛网拦截装置2为内嵌式的栅栏结构，设置在生物反应装置1的出水口9处，防止填料5对出水口9堵塞；
所述的曝气泵3通过管道与曝气盘7连接，所述的进料泵4通过管道与进水口8连接；
所述的进水口8位于出水口9的下方，所述的生物反应装置1的高径比为17:50；
所述的填料5采用聚乙烯材质，填料5的形状为中空圆柱体，包括内中外三层结构，内层为正六边形，正六边形的每个角向外延伸一尾翘，与中间第二层同心圆相连，内层和中间第二层向不同方向延伸出一条条竖棱；最外层为圆形，与第二层同心圆由12条尾翘相连，且其横截面由多个圆弧首尾相连而成。
所述的高盐废水MBBR反应器的运行参数为：
填料填充率为25％-45％，优选35％；
溶解氧浓度为2.0-7.0mg/L，优选3.0-5.0mg/L；
pH为6.0-9.0，优选7.0-7.5；
水温为15-25℃。
实施例2
如实施例1所述的一种高盐废水MBBR反应器，不同的是：
所述的生物反应装置1的高径比为16:48，所述的填料5采用聚丙烯材质。
实施例3
如实施例1所述的一种高盐废水MBBR反应器，不同的是：
所述的生物反应装置1的高径比为18：52，所述的填料5采用聚氨酯材质。
实施例4:
一种以潮间带沉积物为接种体系的高盐废水纯膜MBBR启动方法，包括使用实施例1所述的高盐废水MBBR反应器，包括：向高盐废水纯膜MBBR反应器内进行潮间带沉积物的接种步骤、填料挂膜步骤和生物膜的驯化步骤；
(1)潮间带沉积物预处理：潮间带沉积物取回后，因其中含有大量的无机颗粒，需在接种前进行预处理。将潮间带沉积物用盐水(盐度＝3％)浸润，保证其MLSS为10000mg/L，以乙酸钠、氯化铵、磷酸二氢钾为培养基质，按C：N：P＝100:5:1的比例添加(质量浓度比)，开展三轮烧杯曝气实验。持续观察COD在反应过程中的浓度变化，当COD去除率大于50％时，更换新的废水，第一轮烧杯曝气实验结束；第二轮烧杯实验重复上述操作，当COD去除率大于80％时，再次更换新的废水；以烧杯实验中潮间带沉积物的VSS/TSS约为0.7时作为第三轮烧杯曝气实验的终点。
(2)接种和填料挂膜：结合具体的高盐废水水质特征并根据需要补充营养及常量和微量元素，所述的营养为模拟含盐废水，其中COD为500-2000mg/L，氨氮浓度为25-100mg/L，磷酸盐浓度为5-20mg/L，所述的常量及微量元素如表1所示；向MBBR反应器内接种经过预处理的潮间带沉积物进行挂膜，接种浓度为100mg/L，使填料完全浸没在含有新型接种体的混合液中，充分曝气，使得填料刚好流化，挂膜初期采取间断流的方式，保证系统内具有充足的悬浮态生物量，促进生物膜的形成，进而实现MBBR系统的初步挂膜。
(3)生物膜驯化：待系统内COD去除率大于80％时，改间断流为连续流，更换新的废水，仅留下填料(含填料内部附着的生物膜)，以有机物去除率和混合液浊度(游离微生物细胞密度)来调整进水速率。逐步提高有机负荷，加速生物膜的形成并促进菌群结构优化。
(4)工艺参数优化：在MBBR连续流处理高盐废水的过程中，结合出水水质、生物膜表观变化及镜检结果优化核心运行参数，控制有机负荷2.0-4.0kgCOD/(m 3.d)、水力停留时间12-24h、溶解氧浓度3.0-6.0mg/L、pH值7.0-7.5，以缩短系统启动(挂膜)期，并提高污染物削减效率。
本实施例中MBBR反应器进水盐度为3％、溶解氧浓度为3.0-6.0mg/L、pH为7.0-8.0、水温为20-25℃、填料填充率30％。
试验例1
本按照实施例4的方法对高盐废水进行处理，处理效果图如图3所示。由图3可知，MBBR工艺处理高盐废水启动期内SCOD去除率可以达到84％，氨氮去除率可以达到99％。待系统稳定运行后，优化系统核心参数，调节进水TOC为650mg/L、氨氮浓度为100mg/L，MBBR系统TOC、氨氮去除率分别可以达到95％和98％。
对比例
如实施例4所述，不同的是：
将潮间带沉积物替换为传统好氧活性污泥。
效果实验：
使用传统活性污泥启动MBBR高盐处理系统，进水COD浓度为500mg/L，氨氮浓度为25mg/L，磷酸盐浓度为5mg/L。启动第13天，可以观察到填料表面附有较薄的生物膜，结合出水水质、生物膜表观变化及镜检结果优化核心运行参数，控制有机负荷2.0kgCOD/(m 3.d)、水力停留时间12-24h、溶解氧浓度3.0-6.0mg/L、pH值7.0-7.5。待系统启动30天后，MBBR系统TOC去除率为84.2％；氨氮去除率为57％，其处理效果如图4所示。可以看出，总体效果显著低于接种潮间带沉积物的MBBR系统。

Claims (10)

  1. 一种以潮间带沉积物为接种体系的高盐废水纯膜MBBR启动方法，包括：向高盐废水纯膜MBBR反应器内进行潮间带沉积物的接种步骤、填料挂膜步骤和生物膜的驯化步骤。
  2. 根据权利要求1所述的以潮间带沉积物为接种体系的高盐废水纯膜MBBR启动方法，其特征在于，所述的潮间带沉积物的盐度为1％-4％，优选2.5％-3.5％；
    优选的，所述的潮间带沉积物的VSS/TSS＝0.08-0.18。
  3. 根据权利要求1所述的以潮间带沉积物为接种体系的高盐废水纯膜MBBR启动方法，其特征在于，所述的潮间带沉积物取自潮间湿地表层0-5cm处；
    优选的，潮间带沉积物取回后，在接种前进行预处理，预处理步骤如下：
    将潮间带沉积物置于容器中，用盐度＝3％的盐水浸润；按C：N：P＝100:5:1的比例(质量浓度比)添加乙酸钠、氯化铵和磷酸二氢钾，控制进水COD为500mg/L，氨氮为25mg/L，磷酸盐浓度为5mg/L，控制其污泥浓度MLSS为10000mg/L，作为模拟废水，进行三次曝气；持续观察容器中COD在反应过程中的浓度变化，当COD去除率大于50％时，更换新的模拟废水，第一次曝气结束；重复上述操作进行第二次曝气，当COD去除率大于80％时，再次更换新的模拟废水，第二次曝气结束；重复上述操作进行第三次曝气，当容器中潮间带沉积物的VSS/TSS为0.65-0.75时第三次曝气结束。
  4. 根据权利要求1所述的以潮间带沉积物为接种体系的高盐废水纯膜MBBR启动方法，其特征在于，所述的潮间带沉积物的接种，是在MBBR反应器内投加预处理后的潮间带沉积物。
  5. 根据权利要求1所述的以潮间带沉积物为接种体系的高盐废水纯膜MBBR启动方法，其特征在于，所述的高盐废水MBBR反应器中潮间带沉积物的接种浓度80-120mg/L。
  6. 根据权利要求1所述的以潮间带沉积物为接种体系的高盐废水纯膜MBBR启动方法，其特征在于，所述的填料挂膜步骤，按如下方法进行：
    接种后，使高盐废水MBBR反应器内的填料完全浸没在含有潮间带沉积物的混合液中，充分曝气，使得填料刚好流化，采取间断流的方式，保证高盐废水MBBR反应器内具有充足的悬浮态生物量，促进生物膜的形成，进而实现高盐废水MBBR反应器的挂膜。
  7. 根据权利要求6所述的以潮间带沉积物为接种体系的高盐废水纯膜MBBR启动方法，其特征在于，所述的填料挂膜步骤中对高盐废水MBBR反应器内添加营养及微生物生长所需的常量元素和微量元素；所述的营养为模拟含盐废水，其中按C：N：P＝100:5:1的比例(质量浓度比)添加乙酸钠、氯化铵和磷酸二氢钾，控制进水COD为500-2000mg/L，氨氮为25-100mg/L，磷酸盐浓度为5-20mg/L；
    所述的常量元素和微量元素如表1所示：
    表1 常量及微量元素
    元素浓度(g/L)添加比例(废水量：投加量)CaCl<sub>2</sub>30 1000:1MgSO<sub>4</sub>25 1000:1 FeSO<sub>4</sub>20 1000:1H<sub>3</sub>BO<sub>3</sub>0.05 10000:1 ZnCl<sub>2</sub> 0.05 10000:1CuCl<sub>2</sub>0.05 10000:1 MnSO<sub>4</sub>0.05 10000:1(NH<sub>4</sub>)<sub>6</sub>Mo<sub>7</sub>O<sub>24</sub>0.05 10000:1AlCl<sub>3</sub>0.05 10000:1 CoCl<sub>2</sub>0.05 10000:1NiCl<sub>2</sub>0.05 10000:1
    。
  8. 根据权利要求1所述的以潮间带沉积物为接种体系的高盐废水纯膜MBBR启动方法，其特征在于，所述的生物膜的驯化步骤，按如下方法进行：
    填料挂膜后，待高盐废水MBBR反应器内COD去除率大于80％时，改间断流为连续流，更换新的废水，仅留下填料及填料内部附着的生物膜，依据有机物去除率和混合液浊度(游离微生物细胞密度)来调整进水速率，加速生物膜的形成并促进菌群结构优化。
  9. 根据权利要求1所述的以潮间带沉积物为接种体系的高盐废水纯膜MBBR启动方法，其特征在于，生物膜的驯化步骤之后，还包括工艺参数优化步骤；
    在MBBR连续流处理高盐废水的过程中，结合出水水质、生物膜表观变化及镜检结果优化核心运行参数以缩短系统启动期，并提高污染物削减效率；
    所述的核心运行参数控制为：有机负荷2.0-4.0kgCOD/(m3.d)、水力停留时间12-24h，溶解氧浓度3.0-6.0mg/L，pH＝7.0-7.5。
  10. 一种高盐废水MBBR反应器，其特征在于，包括生物反应装置、流化装置、筛网拦截装置、曝气泵和进料泵；
    所述的生物反应装置为圆柱容器，用于盛放MBBR填料，生物反应装置设置有进水口和出水口；所述的流化装置包括铺满生物反应装置底部的圆形曝气盘和竖直设置在生物反应装置内的机械搅拌装置，流化装置便于均匀曝气以及辅助流化；所述的筛网拦截装置为内嵌式的栅栏结构，设置在生物反应装置的出水口处，防止填料对出水口堵塞；
    所述的曝气泵通过管道与曝气盘连接，所述的进料泵通过管道与进水口连接。
PCT/CN2021/126295 2021-02-24 2021-10-26 一种以潮间带沉积物为接种体系的高盐废水纯膜mbbr启动方法 WO2022179146A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202110206209.3A CN113307379B (zh) 2021-02-24 2021-02-24 一种以潮间带沉积物为接种体系的高盐废水纯膜mbbr启动方法
CN202110206209.3 2021-02-24

Publications (1)

Publication Number Publication Date
WO2022179146A1 true WO2022179146A1 (zh) 2022-09-01

Family

ID=77370555

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2021/126295 WO2022179146A1 (zh) 2021-02-24 2021-10-26 一种以潮间带沉积物为接种体系的高盐废水纯膜mbbr启动方法

Country Status (2)

Country Link
CN (1) CN113307379B (zh)
WO (1) WO2022179146A1 (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115557604A (zh) * 2022-10-25 2023-01-03 江南大学 一种高盐有机废水厌氧处理反应器快速启动的方法

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113307379B (zh) * 2021-02-24 2023-03-14 青岛理工大学 一种以潮间带沉积物为接种体系的高盐废水纯膜mbbr启动方法
CN114573091B (zh) * 2021-11-26 2023-08-29 青岛理工大学 一种电极过滤除菌的纯膜mbbr装置及除菌方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103540555A (zh) * 2013-11-05 2014-01-29 中蓝连海设计研究院 一种制备耐盐微生物菌剂的方法
CN105036312A (zh) * 2015-09-14 2015-11-11 中蓝连海设计研究院 一种高盐废水mbbr处理系统启动方法
CN106675999A (zh) * 2017-01-20 2017-05-17 浙江大学 海洋comammox富集培养装置和方法
CN111115821A (zh) * 2020-01-20 2020-05-08 青岛思普润水处理股份有限公司 基于mbbr的自养脱氮一体化系统及快速启动方法
WO2020263786A1 (en) * 2019-06-27 2020-12-30 Veolia Water Solutions & Technologies Support System and process for removing ammonium from a wastewater stream
CN113307379A (zh) * 2021-02-24 2021-08-27 青岛理工大学 一种以潮间带沉积物为接种体系的高盐废水纯膜mbbr启动方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104724889B (zh) * 2015-03-24 2016-10-26 山东大学 一种降低温室气体排放的污水处理系统及方法
FR3067024B1 (fr) * 2017-06-02 2020-07-31 Veolia Water Solutions & Tech Procede de traitement des eaux dans un systeme comprenant au moins un reacteur sequentiel discontinu et un reacteur a film biologique a lit mobile.

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103540555A (zh) * 2013-11-05 2014-01-29 中蓝连海设计研究院 一种制备耐盐微生物菌剂的方法
CN105036312A (zh) * 2015-09-14 2015-11-11 中蓝连海设计研究院 一种高盐废水mbbr处理系统启动方法
CN106675999A (zh) * 2017-01-20 2017-05-17 浙江大学 海洋comammox富集培养装置和方法
WO2020263786A1 (en) * 2019-06-27 2020-12-30 Veolia Water Solutions & Technologies Support System and process for removing ammonium from a wastewater stream
CN111115821A (zh) * 2020-01-20 2020-05-08 青岛思普润水处理股份有限公司 基于mbbr的自养脱氮一体化系统及快速启动方法
CN113307379A (zh) * 2021-02-24 2021-08-27 青岛理工大学 一种以潮间带沉积物为接种体系的高盐废水纯膜mbbr启动方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115557604A (zh) * 2022-10-25 2023-01-03 江南大学 一种高盐有机废水厌氧处理反应器快速启动的方法

Also Published As

Publication number Publication date
CN113307379B (zh) 2023-03-14
CN113307379A (zh) 2021-08-27

Similar Documents

Publication Publication Date Title
WO2022179146A1 (zh) 一种以潮间带沉积物为接种体系的高盐废水纯膜mbbr启动方法
Sun et al. Wastewater treatment and membrane fouling with algal-activated sludge culture in a novel membrane bioreactor: Influence of inoculation ratios
CN102010094B (zh) 高钙、高盐工业废水处理方法
CN108217939B (zh) 缺氧-好氧移动床生物膜反应系统处理高氨氮废水的启动方法
CN103086572B (zh) 一种高浓度合成制药废水的预处理方法
CN100431982C (zh) 一种在膜生物反应器中抑制膜污染的方法
CN112142199B (zh) 一种提高一体式部分反硝化-厌氧氨氧化耦合脱氮性能的装置及方法
CN108996832B (zh) 一种利用菌剂处理工业废水的工艺
CN104925944A (zh) 脱氮填料及其制备方法和在水体脱氮中的应用
CN102603064A (zh) 一种含氮磷污水同步脱氮除磷的方法
CN110395851A (zh) 基于氮磷捕获和全程自养脱氮的高海拔城镇污水处理方法
CN104609542A (zh) 一种培养耐盐好氧活性污泥的方法
CN108117221A (zh) 一种反渗透浓水的处理方法
CN104355404B (zh) 一种快速实现生物滤池厌氧氨氧化的方法与装置
CN102092846A (zh) 一种同步脱氮除碳好氧颗粒污泥的连续培养方法
CN101781056B (zh) 造纸废水的处理方法
CN102776140A (zh) 一株耐冷假单胞菌Den-05及其筛选方法和应用
WO2015066966A1 (zh) 一种启动高盐废水好氧生化处理系统的方法
CN110699285A (zh) 一种巴利阿里假单胞菌及其在处理垃圾渗滤液膜浓缩液中的应用
CN111018101B (zh) 一种膜生物膜培养驯化工艺及处理高盐废水用膜生物膜反应装置
CN116924605A (zh) 一种矿山酸性废水生态处理系统及其处理方法
CN103373769A (zh) 一种工业循环水的生物净化方法
CN112574921A (zh) 利用餐厨垃圾制备有氧反硝化复合菌剂的方法及其应用
CN106430590A (zh) 一种基于两级ao装置的高电导率废水处理方法
CN111362425A (zh) 一种微电解强化硫酸盐还原菌处理酸性矿山废水的方法及微电解生物反应器

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 21927570

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 21927570

Country of ref document: EP

Kind code of ref document: A1