WO2022143578A1 - 一种生物样本处理系统及处理方法 - Google Patents

一种生物样本处理系统及处理方法 Download PDF

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Publication number
WO2022143578A1
WO2022143578A1 PCT/CN2021/141835 CN2021141835W WO2022143578A1 WO 2022143578 A1 WO2022143578 A1 WO 2022143578A1 CN 2021141835 W CN2021141835 W CN 2021141835W WO 2022143578 A1 WO2022143578 A1 WO 2022143578A1
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WIPO (PCT)
Prior art keywords
turntable
reagent tube
rotate
drive
driving
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PCT/CN2021/141835
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English (en)
French (fr)
Inventor
张昊
孙振波
房瑜
魏金文
张准
乔月华
王林
Original Assignee
南京金斯瑞生物科技有限公司
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Publication of WO2022143578A1 publication Critical patent/WO2022143578A1/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/24—Apparatus for enzymology or microbiology tube or bottle type
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors

Definitions

  • the invention belongs to the technical field of biological sample processing, and relates to a biological sample processing system and a processing method.
  • the purpose of the present invention is to provide a biological sample processing system and processing method.
  • the present invention provides a biological sample processing system, the system includes: a turntable rotating device and a sample adding device, one or more of a liquid suction device and a magnetic suction device connected together by a support structure; wherein,
  • the turntable rotating device has a mixing function, and the turntable rotating device is used to rotate the reagent tube to a set station and mix the biological samples in the reagent tube.
  • the turntable rotating device includes a rotating turntable mechanism and a mixing uniform organization;
  • the rotating turntable mechanism mainly includes: a turntable, a main shaft, a bearing seat, a bearing, a support plate, a turntable driving device, etc.; Rotate in the axial direction.
  • the main shaft is connected with the turntable, and the main shaft passes through the inner hole of the bearing and is mounted on the support plate through the bearing seat.
  • the upper and lower ends of the bearing seat are provided with a first bearing and a second bearing; the outer rings of the first bearing and the second bearing are respectively connected with the bearing seat; the main shaft passes through the first bearing and the second bearing. the inner ring of the second bearing.
  • the turntable driving device drives the turntable to realize circular rotation, and judges the rotation angle of the turntable through the position sensor.
  • the mixing mechanism is fixed on the turntable, and the number can be set to one or more as required, and is distributed along the circumference of the turntable to provide a plurality of rotating mixing stations.
  • the mixing mechanism mainly includes a mixing cup and a mixing driving device.
  • the mixing drive device drives the mixing cup to rotate along the axial direction; the axial rotation of the mixing cup and the axial rotation of the turntable are independent of each other.
  • the mixing cup rotates, the biopolymer in the reagent tube installed on the mixing cup is driven to mix; and/or, the biopolymer directly added into the mixing cup is driven to mix.
  • the turntable driving device may be a rotary motor that drives the turntable to rotate through a synchronous pulley set;
  • the synchronous pulley set includes: a driving pulley, a driven pulley, and a synchronous belt; wherein, the driven pulley is fixed on the on the main shaft; the driven pulley is connected to the driving pulley through the synchronous belt; the driving pulley is fixed on the rotating motor, and the driven pulley is driven to rotate by the rotation of the rotating motor, and further drive the turntable to rotate;
  • the turntable driving device can be a rotary motor that directly drives the main shaft to rotate; the rotary motor is directly connected to the main shaft through a coupling to drive the turntable to rotate;
  • the turntable driving device may be a rotary motor to drive the turntable to rotate through a gear set; the gear set includes: a first driving gear and a first driven gear.
  • the rotating electrical machine is connected with the first driving gear, and the first driven gear is connected with the main shaft; the first driving gear is meshed with the first driven gear, and the first driving gear is with the first driven gear.
  • the rotary motion of the rotary motor is directly transmitted through the tooth meshing, which further drives the turntable to rotate.
  • the rotating turntable mechanism is optionally equipped with a position sensor, and the function of the position sensor can be realized by a code disk and an optocoupler;
  • the code disk is a circular sheet with an elongated notch and is fixed on the main shaft;
  • the optocoupler is fixed On the support plate, the initial position of the turntable is confirmed by whether the code disc blocks the optical signal of the optocoupler in the process of rotating with the main shaft, and the position of each mixing station on the turntable is controlled by controlling the rotation steps of the rotating motor. .
  • the optional structure of the rotating turntable mechanism includes a slip ring; the slip ring can be purchased in the market; the slip ring is connected with the main shaft; the main shaft is a hollow shaft; the slip ring includes a rotor and a stator, and the rotor During the rotation process, it can still transmit electrical signals with the stator;
  • the first wire harness derived from the slip ring rotor passes through the inner hole of the main shaft to the turntable, and is connected to the control circuit board of the first drive motor; the outer casing of the slip ring is the stator, which is limited and fixed by the stopper pin , which can lead to a fixed second wire harness.
  • the mixing cup can be directly fixed on the drive shaft of the first driving motor, the first driving motor is fixed on the lower side of the turntable, and when the first driving motor rotates, the mixing cup is driven to rotate; And/or, the first driving motor is connected with the second driving gear, and the second driven gear is driven to rotate through the meshing of the second driving gear, thereby driving the mixing cup to rotate;
  • each second driven gear is provided with a mixing cup
  • the mixing cup and the second driven gear are fixed on the small shaft; the small shaft is installed with two miniature bearings at the corresponding stations of the turntable, which pass through the inner hole of the miniature bearings and are indirectly connected to the turntable, Thereby, the second driven gear rotates relative to the circumference of the turntable.
  • the sample adding device cooperates with the turntable rotating device with a mixing function to add samples to the reagent tubes;
  • the liquid suction device cooperates with the turntable rotating device with a mixing function to add samples to the reagent tubes.
  • the liquid is sucked;
  • the magnetic suction device cooperates with the turntable rotating device with the mixing function to perform magnetic suction operation on the magnetic beads in the reagent tube.
  • the sample adding device includes: a sample adding needle fixing plate, a guide rail, a second driving motor, a first screw rod, and a sample adding needle;
  • the sample addition needles is provided on the sample addition needle fixing plate to add samples to the reagent tube.
  • the sample addition device drives the first sample through the second drive motor.
  • the screw rod drives the sample needle fixing plate to move up and down under the guidance of the guide rail, and further drives the sample needle into the reagent tube for sample addition.
  • the liquid suction device includes: a third driving motor, a fourth driving motor, a liquid suction needle, a liquid suction arm, and a second screw rod;
  • the suction needle is fixed on the suction arm of the suction device, and the suction device drives the second screw rod through the third drive motor to drive the suction arm to rotate;
  • the fourth drive motor drives the second screw rod to drive the suction arm to move vertically up and down in the axial direction; when the reagent tube needs to suction liquid, the third drive motor rotates the suction needle to the position of the reagent tube.
  • the fourth drive motor drives the aspiration needle to move into the reagent tube, and when the aspiration needle moves to a set position, the liquid in the reagent tube is sucked out.
  • the magnetic suction device includes: a magnetic suction device synchronous belt, a fifth drive motor, a magnet assembly, a main synchronous wheel, and a secondary synchronous wheel;
  • the fifth drive motor is directly connected to the main synchronizing wheel, and the main synchronizing wheel drives the secondary synchronizing wheel to rotate through the synchronous belt of the magnetic attraction device, thereby driving the magnet assembly to move, close to or away from the reagent tube, and perform the biological sample analysis. Magnetic attraction.
  • the present invention also provides a biological sample processing method, which adopts the above processing system, and specifically includes the following steps:
  • Step 1 Insert multiple reagent tubes containing biological samples into the mixing cup;
  • Step 2 The turntable rotating device rotates the reagent tube to the set sample adding position, and the sample adding device adds the reagent into the reagent tube;
  • Step 3 Start the motor of the turntable rotating device, forward and reverse the reagent tube, and fully mix the biological sample in the reagent tube;
  • Step 4 The mixed biological sample reaches the set magnetic suction position through the rotation of the turntable, and the magnetic suction device adsorbs the magnetic beads in the reagent tube;
  • Step 5 After the adsorption is completed, the liquid suction device sucks out the waste liquid in the reagent tube;
  • Step 6 Through the rotation of the turntable, multiple biological samples can be processed continuously.
  • the beneficial effects of the present invention include: the biological sample processing system of the present invention can add one or more functions such as sample addition, suction, and magnetic suction on the basis of sample mixing, and can process multiple samples at one time, which greatly simplifies the instrument and save time. At the same time, compared with the existing manual operation, the operation process of the experimenter is greatly simplified, and the quality of the extracted product is greatly improved due to the stability of the machine.
  • FIG. 1 is a schematic diagram of the overall structure of the turntable rotating device in the present invention.
  • FIG. 2 is a schematic diagram of the overall structure of the turntable rotating device in the present invention from a first perspective.
  • FIG. 3 is a schematic diagram of a second perspective view of the overall structure of the turntable rotating device in the present invention.
  • FIG. 4 is a schematic diagram of the rotary motor of the turntable rotating device in the present invention directly driving the turntable to rotate.
  • FIG. 5 is a schematic diagram of the rotary motor of the turntable rotating device in the present invention driving the turntable to rotate through a gear set.
  • FIG. 6 is a cross-sectional view of the rotation of the main shaft of the turntable rotating device in the present invention.
  • FIG. 7 is a schematic diagram of the wire harness leading out of the slip ring of the turntable rotating device in the present invention.
  • FIG. 8 is a schematic diagram of the mixing mode of the gear drive driving the mixing cup of the turntable rotating device in the present invention.
  • FIG. 9 is a schematic structural diagram of the mixing cup relative to the turntable in the mixing mode of the turntable rotating device gear drive driving the mixing cup according to the present invention.
  • FIG. 10 is a schematic diagram of the mixing method of the motor of the turntable rotating device directly driving the mixing cup according to the present invention.
  • Figure 11 is a schematic top view of the main devices of the processing system of the present invention.
  • FIG. 12 is a schematic diagram of the overall structure of the processing system of the present invention.
  • Figure 13 is a schematic structural diagram of the sample adding device of the present invention.
  • Figure 14 is a schematic structural diagram of the liquid suction device of the present invention.
  • 15 is a schematic structural diagram of the magnetic attraction device of the present invention.
  • Figures 16a and 16b are schematic diagrams of two usage methods of the magnetic attraction device of the present invention.
  • 100-Rotary turntable mechanism 110-Turntable, 161-Rotary motor, 121-Code disc, 122-Optocoupler, 162-Synchronous belt, 163-Active pulley, 164-Driven pulley, 153-Block pin, 165- Coupling, 130-main shaft, 167-first driven gear, 166-first driving gear, 140-bearing seat, 141-first bearing, 142-second bearing, 150-slip ring, 151-first wire harness , 152-second wiring harness, 160-turntable drive device;
  • 200-mixing mechanism 212-mixing cup, 214-second driven gear, 213-second driving gear, 211-first drive motor, 215-small shaft, 216-first miniature bearing, 217-second Miniature bearing, 218-shaft sleeve, 210-mixing drive device;
  • 600-Sampling device 601-Sampling needle fixing plate, 602-Guide rail, 603-Second drive motor, 604-First screw rod, 605-Sampling needle;
  • 700-liquid suction device 701-third drive motor, 702-fourth drive motor, 703-liquid suction needle, 704-liquid suction arm, 705-second screw rod;
  • the invention discloses a biological sample processing device, more specifically a plasmid or protein purification device.
  • the system mainly includes a rotating turntable device with a mixing function.
  • a sample adding device, a liquid suction device, and a magnetic suction device are integrated on the turntable rotating device, which can provide one or more functions such as sample adding, liquid suction, mixing, and magnetic suction.
  • the biological sample processing system in this embodiment includes: a turntable rotating device 500 with a mixing function, a sample adding device 600 , a liquid suction device 700 , and a magnetic suction device 800 connected together by a support structure 900 .
  • the biological sample processing system of the present invention can also only integrate any one or any combination of the sample adding device 600 , the liquid suction device 700 and the magnetic suction device 800 on the turntable rotating device.
  • the turntable rotating device 500 in this embodiment is used to rotate the reagent tube 220 to a set station and mix the biological samples in the reagent tube 220 evenly; its structure is shown in FIGS. 1-10 , including two major mechanisms : Rotate the turntable mechanism 100 and the mixing mechanism 200.
  • the rotating turntable mechanism 100 includes: a turntable 110 , a main shaft 130 , a bearing seat 140 , a first bearing 141 , a second bearing 142 , a turntable driving device 160 , and a support plate 300 .
  • the rotary turntable mechanism 100 is a disc-shaped structure that can rotate around an axis. There are 16 workstations distributed along the outer circumference of the disc, and 16 samples can be stored. The samples can be transferred to a preset position according to process requirements; the rotary turntable mechanism 100 provides power to drive the turntable 110 to rotate in the axial direction through the turntable driving device 160 .
  • the bearing seat 140 is fixed on the upper side of the support plate 300, the upper and lower ends of the bearing seat 140 are respectively equipped with a first bearing 141 and a second bearing 142, and the outer rings of the first bearing 141 and the second bearing 142 are respectively connected with the bearing seat 140;
  • the main shaft 130 passes through the inner rings of the first bearing 141 and the second bearing 142 , the upper side of the main shaft 130 is connected to the turntable 110 , and the lower side of the main shaft 130 is connected to the turntable driving device 160 ; and the turntable 110 is only one that rotates around the shaft relative to the support plate 300 degrees of freedom.
  • the turntable driving device 160 can be a rotary motor 161 connected to the main shaft 130 through a synchronous pulley set to drive the turntable 110 to rotate;
  • the synchronous pulley set includes: a driving pulley 163, a driven pulley 164, and a synchronous belt 162.
  • the synchronous pulley set exists The reduction ratio of 1:6 is used to reduce the rotational speed of the turntable 110 and amplify the output torque of the rotary motor 161 by 6 times.
  • the driven pulley 164 is directly fixed on the main shaft 130, and is connected with the driving pulley 163 through the timing belt 162; the driving pulley 163 is directly fixed on the rotating motor 161, and the driven pulley 164 is driven to rotate by the rotation of the rotating motor 161, and further Drive the turntable 110 to rotate;
  • the turntable driving device 160 may be a rotary motor 161 connected to the main shaft 130 through a gear set to drive the turntable 110 to rotate;
  • the gear set includes: a first driving gear 166, a first driven gear 167, and the reduction ratio of the gear set is 1:6;
  • a driving gear 166 is fixed on the rotating motor 161, and by meshing with the first driven gear 167, it drives the main shaft 130 to rotate, and further drives the turntable 110 to rotate;
  • the turntable driving device 160 may also be a rotary motor 161 directly connected to the main shaft 130 through a coupling 165 to drive the turntable 110 to rotate.
  • a code disc 121 with an elongated notch is fixed on the lower side of the main shaft 130, and an optocoupler 122 is fixed on the corresponding position of the support plate 300.
  • the optocoupler receiving end receives the optocoupler.
  • the signal sent by the transmitting end is the initial position of the turntable 110; the rotating motor 161 drives the turntable 110 to rotate by a fixed angle, and further drives the mixing cup 212 to reach the set position.
  • a slip ring 150 is fixed on the main shaft 130, and the slip ring 150 can be purchased in the market;
  • the main shaft 130 is a hollow shaft;
  • the first wire harness 151 derived from the rotor of the slip ring 150 passes through the inner hole of the main shaft 130 to the turntable 110, and is connected with the first drive
  • the control circuit board 400 of the motor 211 is connected and rotates together with the turntable 110 ;
  • the outer casing of the slip ring 150 is the stator, which is limited and fixed by the stopper pin 153 , and the second wire harness 152 derived from it is fixed, so that the inside of the turntable 110 is fixed. After the wire harness is drawn out of the outside, no winding phenomenon will occur.
  • the 16 mixing mechanisms 200 are evenly distributed on the circumference of the outer ring of the turntable 110 .
  • the corresponding mixing mechanism 200 rotates to drive the sample to mix;
  • the first driving motor 211 directly drives the mixing cup 212 for mixing, which means that the mixing cup 212 is directly fixed on the drive shaft of the first driving motor 211 by screws; When the driving motor 211 rotates, it drives the mixing cup 212 to rotate; the reagent tube 220 can also be installed on the mixing cup 212. When the first driving motor 211 rotates, it drives the reagent tube 220 to rotate and mix evenly.
  • the uniform speed does not exceed 1400r/min, and can realize forward and reverse.
  • the first driving motor 211 can also drive the two mixing cups 212 to rotate through the meshing of the second driving gear 213 and the two second driven gears 214; the first driving motor 211 is fixed on the lower side of the turntable 110 and passes through the turntable 110 and the The two driving gears 213 are connected; two second driven gears 214 are distributed on the outside of the second driving gear 213 to mesh with it.
  • the homogeneous cup 212 is rotated.
  • the 16 second driven gears 214 are evenly distributed on the outside of the 8 second driving gears 213 along the circumference of the turntable 110 ; the 8 second driving gears 213 are controlled by the 8 first driving motors 211 respectively.
  • the two second driven gears 214 are driven to rotate, and the second driven gear 214 and the second driving gear 213 have an acceleration ratio of 1:2, thereby amplifying the rotational speed of the mixing cup 211 and achieving better mixing.
  • the maximum mixing speed does not exceed 1400 r/min, and the two second driven gears 214 controlled by the same first driving motor 211 have the same rotational speed and opposite directions;
  • the mixing cup 212 and the second driven gear 214 are directly fixed on the small shaft 215 and are located on the upper side of the turntable 110 ; two miniature bearings 216 and 217 are installed at the 16 stations of the turntable 110 .
  • a shaft is installed between the second driven gear 214 and the first miniature bearing 216.
  • the sleeve 218; the second driven gear 214 can rotate relative to the turntable 110 to drive the biopolymer in the mixing cup 212 to mix.
  • the sample adding device 600 in this embodiment can realize the up and down Z-direction movement of the sample adding needle 605 during use, and can also rotate around the Z-axis.
  • the sample adding device 600 cooperates with the turntable rotating device 500 with the mixing function.
  • the sample adding device 600 of the present invention cooperates with the turntable rotating device 500 to add samples to the reagent tube 220.
  • the sample adding needle fixing plate 601 is provided with at least one sample adding needle 605.
  • the turntable rotates
  • the mechanism 500 transfers the reagent tube 220 to be sampled to the bottom of the sample adding needle 605 through the rotating motor 161
  • the sample adding device 600 drives the first screw 604 through the second driving motor 603 , and drives the sample adding under the guiding action of the guide rail 602 .
  • the needle fixing plate 601 is moved up and down, and the sample adding needle 605 is further penetrated into the reagent tube 220 to add sample.
  • sample adding needle fixing plate 601 is provided with a plurality of sample adding needles 605, and different liquids can be added to the reagent tube 220 by sucking a quantitative reagent from the prepared reagent bottle.
  • sample adding device 600 may also adopt the form including but not limited to a synchronous belt, or realize the device through a gantry mechanism, and other structures capable of realizing the sample adding function.
  • the liquid suction device 700 in this embodiment cooperates with the turntable rotating device 500 with a mixing function to absorb the liquid in the reagent tube 220; its structure includes: a third driving motor 701, a fourth driving motor 702, a liquid suction needle 703, suction arm 704, second screw 705;
  • the liquid suction needle 703 is fixed on the liquid suction device 700, and the liquid suction device 700 can also provide two degrees of freedom of movement.
  • the fourth drive motor 702 drives the suction arm 704 to move vertically up and down by driving the second screw rod 705; when the reagent tube 220 needs to suction liquid, the turntable rotating device 500 passes the reagent tube 220 that needs to discharge waste liquid through the
  • the rotation motor 161 rotates to a predetermined position
  • the third drive motor 701 rotates the aspiration needle 703 to the top of the reagent tube 220
  • the fourth drive motor 702 drives the aspiration needle 703 to move into the reagent tube 220, when the aspiration needle 703 moves When reaching the set position, under the action of the external pump body, the liquid in the reagent tube 220 is sucked out.
  • the liquid suction device 700 removes the liquid suction needle 703 from the reagent tube 220 and sends it into an external cleaning tank to clean the liquid suction needle 703 .
  • the liquid suction device 700 removes the liquid suction needle 703 from the cleaning tank, and performs the waste liquid suction treatment of other reagent tubes 220, so as to prevent cross infection.
  • the structure of the magnetic attraction device 800 in this embodiment includes: a magnetic attraction device synchronous belt 802, a fifth drive motor 801, a magnet assembly 803, a master synchronizing wheel 804, and a slave synchronizing wheel 805; the magnetic attraction device 800 can realize the For radial movement, the magnetic attraction device 800 cooperates with the turntable rotating device 500 with a mixing function, and the magnet assembly 803 is placed outside the reagent tube 220 that needs to be magnetically attracted, and the magnetic attraction operation is performed on the magnetic beads in the reagent tube 220;
  • the turntable rotating device 500 rotates the reagent tube 220 that needs to be magnetically attracted by the rotating motor 161 to the position facing the magnetic attracting device 800 , and the fifth driving motor 801 It is directly connected with the main synchronizing wheel 804.
  • the main synchronizing wheel 804 drives the secondary synchronizing wheel 805 to rotate through the synchronous belt 802 of the magnetic attraction device, thereby driving the magnet assembly 803 to move back and forth, approaching or away from the reagent tube 220.
  • the magnet assembly 803 When the magnet assembly 803 is close to the reagent The outer wall of the tube 220, the magnetic beads in the sample are sucked out of the reagent tube 220, as shown in Figure 16a; the other is to use the internal magnetic suction method, and the magnet assembly 803 is inserted into the reagent tube from right above the reagent tube 220, and the The magnetic beads in the sample are aspirated from the reagent tube 220, as shown in Figure 16b. Further, after the magnetic beads are magnetically sucked out of the reagent tube 220 , the liquid suction device 700 drives the liquid suction needle 703 to suck away the waste liquid in the reagent tube 220 .
  • a cleaning device 1000 is further included for cleaning the outer wall of the liquid aspiration needle 703 .
  • the cleaning device 1000 includes a cleaning tank, a first diaphragm pump, and a second diaphragm pump; wherein, the suction needle 703 moves into the cleaning tank, and the side wall of the cleaning tank is provided with at least one small hole, which communicates with the first diaphragm through a pipeline.
  • the pump is connected for spraying cleaning liquid to clean the suction needle 703; the second diaphragm pump is used for drawing the produced cleaning liquid away from the cleaning tank.
  • the liquid suction device is in the form of a TIP head, the cleaning device 1000 may not be provided.
  • the cleaning device 1000 can be implemented with other structures that can achieve the same function.
  • Step 1 Insert multiple reagent tubes containing biological samples into the mixing cup;
  • Step 2 The turntable rotating device rotates the reagent tube to the set sample adding position, and the sample adding device adds the reagent into the reagent tube;
  • Step 3 Start the motor of the turntable rotating device, forward and reverse the reagent tube, and fully mix the biological sample in the reagent tube;
  • Step 4 The mixed biological sample reaches the set magnetic suction position through the rotation of the turntable, and the magnetic suction device adsorbs the magnetic beads in the reagent tube;
  • Step 5 After the adsorption is completed, the liquid suction device sucks out the waste liquid in the reagent tube;
  • Step 6 Through the rotation of the turntable, multiple biological samples can be processed continuously.

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Abstract

本发明公开了一种生物样本处理系统，更具体的为质粒或者蛋白纯化装置。该系统主要包括带有混匀功能的旋转转盘装置。在转盘旋转装置上集成了加样装置、吸液装置、磁吸装置，可提供加样、吸液、混匀、磁吸等一个或多个功能，一次可以处理多种样本，极大地简化了仪器和节省了时间。同时与现有的手动操作相比，极大地简化了实验人员的操作流程，由于机器的稳定性，极大地提高了提取出来的产品的质量。本发明还提出了一种生物样本处理方法。

Description

一种生物样本处理系统及处理方法
本申请要求申请日为2020年12月28日、申请号为202011579857.5、发明名称为“一种生物样本处理系统及处理方法”的中国发明专利申请的优先权。
技术领域
本发明属于生物样本处理技术领域，涉及一种生物样本处理系统及处理方法。
背景技术
在现代生物工程中，一般将多种试剂进行混匀反应，在此过程中需要将试剂或者样本进行加样、转移等操作，在进行磁珠处理时还需要对磁珠进行磁吸。现有仪器中混匀与其它操作不能同时进行，同时，现有的磁珠类生物样品基本通过实验人员手动处理，处理流程繁琐，单次处理数量少，耗时长，在操作过程中对人的操作要求较高，容易出错。
发明内容
为了解决现有技术存在的不足，本发明的目的是提供一种生物样本处理系统及处理方法。
本发明提出了一种生物样本处理系统，所述系统包括：通过支撑结构连接在一起的转盘旋转装置和加样装置、吸液装置以及磁吸装置中的一种或多种；其中，
所述转盘旋转装置带混匀功能，所述转盘旋转装置用于将试剂管旋转至设定的工位，并将试剂管中的生物样本混匀；所述转盘旋转装置包括旋转转盘机构和混匀机构；
所述旋转转盘机构主要包括：转盘、主轴、轴承座、轴承、支撑板、转盘驱动装置等组成；所述旋转转盘机构为可绕轴旋转的盘形结构，其通过转盘驱动装置提供动力带动转盘沿轴向旋转。
所述主轴与转盘连接，主轴穿过轴承内孔，通过轴承座安装在支撑板上。
所述轴承座上下两端设有第一轴承和第二轴承；所述第一轴承和所述第二轴承的外圈分别与所述轴承座连接；所述主轴穿过所述第一轴承和所述第二轴承的内圈。
所述转盘驱动装置带动转盘实现圆周转动，并通过位置传感器判断转盘的旋转角度。
所述混匀机构固定在转盘上，数量根据需要可设置为一个或多个，并沿所述转盘的圆周分布，提供多个旋转混匀工位。
所述混匀机构主要包括混匀杯和混匀驱动装置。混匀驱动装置驱动混匀杯沿轴向旋转运动；混匀杯的轴向旋转和转盘的轴向旋转相互独立。
所述混匀杯旋转时，带动安装在混匀杯上的试剂管内的生物聚合物混匀；和/或，带动直接加入混匀杯内的生物聚合物混匀。
所述转盘驱动装置可以为旋转电机通过同步带轮组驱动转盘旋转；所述同步带轮组包 括：主动带轮、从动带轮、同步带；其中，所述从动带轮固定在所述主轴上；所述从动带轮通过所述同步带与所述主动带轮连接；所述主动带轮固定在旋转电机上，通过所述旋转电机的转动带动所述从动带轮转动，进一步带动转盘转动；
所述转盘驱动装置可以为旋转电机直接驱动主轴旋转；所述旋转电机通过联轴器与主轴直接连接，驱动转盘转动；
所述转盘驱动装置可以为旋转电机通过齿轮组驱动转盘旋转；所述齿轮组包括：第一主动齿轮、第一从动齿轮。所述旋转电机与所述第一主动齿轮连接，所述第一从动齿轮与所述主轴连接；所述第一主动齿轮与第一从动齿轮啮合，第一主动齿轮与第一从动齿轮直接通过齿啮合传递旋转电机的旋转运动，进一步带动转盘转动。
所述旋转转盘机构可选的装有位置传感器，位置传感器的功能可通过码盘和光耦实现；所述码盘为圆形薄片并设有细长缺口，固定在主轴上；所述光耦固定在所述支撑板上，通过码盘在随着主轴转动过程中是否遮挡光耦的光信号来确认转盘的初始位置，通过控制旋转电机的转动步数，控制转盘上各混匀工位的位置。
所述旋转转盘机构可选的结构包括滑环；所述滑环可于市场购得；所述滑环与主轴连接；所述主轴为空心轴；所述滑环包括转子和定子两部分，转子转动过程中，仍能与定子相互传递电信号；
所述滑环转子导出的第一线束通过所述主轴内孔穿到转盘上，与第一驱动电机的控制电路板连接；所述滑环的外壳即定子，其通过挡销限位固定不动，可引出固定不动的第二线束。
所述混匀杯可以直接固定在第一驱动电机的驱动轴上，所述第一驱动电机固定在所述的转盘的下侧，当所述第一驱动电机转动时，带动混匀杯旋转；和/或，所述第一驱动电机与所述第二主动齿轮连接，通过第二主动齿轮啮合带动第二从动齿轮转动，从而带动混匀杯旋转；
所述第二主动齿轮外侧分布着两个第二从动齿轮与之啮合；每个第二从动齿轮上装有一个混匀杯；
所述混匀杯和第二从动齿轮固定在小轴上；所述小轴在转盘的相应工位处安装有两个微型轴承，穿过所述微型轴承内孔，间接地与转盘连接，从而实现第二从动齿轮相对转盘圆周转动。
所述加样装置与所述带混匀功能的转盘旋转装置配合，对所述试剂管进行加样；所述吸液装置与所述带混匀功能的转盘旋转装置配合，对所述试剂管的液体进行吸取；所述磁吸装置与所述带混匀功能的转盘旋转装置配合，对所述试剂管中的磁珠进行磁吸操作。
所述加样装置包括：加样针固定板、导轨、第二驱动电机、第一丝杆、加样针；
其中，所述加样针固定板上设有至少一根所述加样针，给试剂管加样，当所述试剂管需要加样时，所述加样装置通过第二驱动电机驱动第一丝杆，在导轨的导向作用下带动加样针固定板实现上下运动，进一步将加样针深入试剂管内，进行加样。
所述吸液装置包括：第三驱动电机、第四驱动电机、吸液针、吸液臂、第二丝杆；
其中，所述吸液针固定在所述吸液装置的吸液臂上，所述吸液装置通过所述第三驱动电机驱动第二丝杆带动吸液臂转动；所述吸液装置通过所述第四驱动电机驱动第二丝杆带动吸液臂轴向竖直上下移动；当所述试剂管需要吸液时，所述第三驱动电机将所述吸液针旋转到所述试剂管的上方，所述第四驱动电机驱动所述吸液针向试剂管内移动，当吸液针运动到设定位置时，将试剂管内的液体吸出。
所述磁吸装置包括：磁吸装置同步带、第五驱动电机、磁铁组件、主同步轮、从同步轮；
其中，所述第五驱动电机与主同步轮直接相连，所述主同步轮通过所述磁吸装置同步带带动从同步轮转动，从而带动磁铁组件运动，靠近或者远离试剂管，对生物样品进行磁吸。
本发明还提出了一种生物样本处理方法，所述方法采用上述的处理系统，具体包括以下步骤：
步骤1：将多个装有生物样本的试剂管分别插入混匀杯上；
步骤2：转盘旋转装置将试剂管转动到设定的加样位，加样装置将试剂加入试剂管内；
步骤3：启动转盘旋转装置的电机，正反转试剂管，将生物样本在试剂管内充分混匀；
步骤4：将完成混匀的生物样本通过转盘的转动到达设定的磁吸位，磁吸装置对试剂管内的磁珠进行吸附；
步骤5：完成吸附后，吸液装置将试剂管内的废液吸出；
步骤6：通过转盘的转动，可以连续处理多个生物样本。
本发明的有益效果包括：本发明生物样本处理系统可以在样本混匀的基础上加入加样、吸取、磁吸等一种或多种功能，一次可以处理多种样本，极大的简化了仪器和节省了时间。同时与现有的手动操作相比，极大地简化了实验人员的操作流程，由于机器的稳定性，极大地提高了提取出来的产品的质量。
附图说明
图1是本发明中转盘旋转装置整体结构简图。
图2是本发明中转盘旋转装置整体结构第一视角示意图。
图3是本发明中转盘旋转装置整体结构第二视角示意图。
图4为本发明中转盘旋转装置的旋转电机直接驱动转盘转动的示意图。
图5为本发明中转盘旋转装置的旋转电机通过齿轮组驱动转盘转动的示意图。
图6是本发明中转盘旋转装置主轴旋转的剖视图。
图7是本发明中转盘旋转装置滑环引出线束的示意图。
图8是本发明中转盘旋转装置齿轮传动驱动混匀杯混匀方式的示意图。
图9是本发明中转盘旋转装置齿轮传动驱动混匀杯混匀方式的混匀杯相对转盘的结构示意图。
图10是本发明中转盘旋转装置电机直接驱动混匀杯混匀方式的示意图。
图11是本发明处理系统主要装置的俯视简图。
图12是本发明处理系统整体结构示意图。
图13是本发明加样装置结构示意图。
图14是本发明吸液装置结构示意图。
图15是本发明磁吸装置结构示意图。
图16a、16b是本发明磁吸装置两种使用方法示意图。
图1-16中：
100-旋转转盘机构、110-转盘、161-旋转电机、121-码盘、122-光耦、162-同步带、163-主动带轮、164-从动带轮、153-挡销、165-联轴器、130-主轴、167-第一从动齿轮、166-第一主动齿轮、140-轴承座、141-第一轴承、142-第二轴承、150-滑环、151-第一线束、152-第二线束、160-转盘驱动装置；
200-混匀机构、212-混匀杯、214-第二从动齿轮、213-第二主动齿轮、211-第一驱动电机、215-小轴、216-第一微型轴承、217-第二微型轴承、218-轴套、210-混匀驱动装置；
220-试剂管；
300-支撑板；
400-控制电路板；
500-转盘旋转装置；
600-加样装置、601-加样针固定板、602-导轨、603-第二驱动电机、604-第一丝杆、605-加样针；
700-吸液装置、701-第三驱动电机、702-第四驱动电机、703-吸液针、704-吸液臂、705-第二丝杆；
800-磁吸装置、801-第五驱动电机、802-磁吸装置同步带、803-磁铁组件、804-主同步轮、805-从同步轮；
900-支撑结构；
1000-清洗装置。
具体实施方式
结合以下具体实施例和附图，对本发明作进一步的详细说明。实施本发明的过程、条件、实验方法等，除以下专门提及的内容之外，均为本领域的普遍知识和公知常识，本发明没有特别限制内容。
本发明公开了一种生物样本处理装置，更具体的为质粒或者蛋白纯化装置。该系统主要包括带有混匀功能的旋转转盘装置。在转盘旋转装置上集成了加样装置、吸液装置、磁吸装置，可提供加样、吸液、混匀、磁吸等一个或多个功能。
本实施例中的生物样本处理系统，系统包括：通过支撑结构900连接在一起的带混匀功能的转盘旋转装置500、加样装置600、吸液装置700、磁吸装置800。实际中，本发明生物样本处理系统也可以仅在转盘旋转装置上集成加样装置600、吸液装置700、磁吸装置800中的任意一个或任意几个组合。
本实施例中的转盘旋转装置500用于将试剂管220旋转至设定的工位，并将试剂管220中的生物样本混匀；其结构如图1-图10所示，包括两大机构：旋转转盘机构100和混匀机构200。
本实施例中旋转转盘机构100包括：转盘110、主轴130、轴承座140、第一轴承141、第二轴承142、转盘驱动装置160、支撑板300。
旋转转盘机构100为可绕轴旋转的盘形结构，沿圆盘外侧圆周一圈分布16个工位，可存放16个样本，样本可根据工艺需求转到预设的位置；所述旋转转盘机构100通过转盘驱动装置160提供动力带动转盘110沿轴向旋转。
轴承座140固定在支撑板300的上侧，轴承座140上下两端各装有第一轴承141和第二轴承142，第一轴承141和第二轴承142的外圈分别与轴承座140连接；
主轴130穿过第一轴承141和第二轴承142的内圈，主轴130上侧与转盘110连接，主轴130下侧与转盘驱动装置160连接；进而转盘110相对支撑板300只有绕轴旋转的一个自由度。
转盘驱动装置160可以为旋转电机161通过同步带轮组与主轴130连接，驱动转盘110旋转；同步带轮组包括：主动带轮163、从动带轮164、同步带162，同步带轮组存在1:6的减速比用于降低转盘110的转速，并将旋转电机161的输出转矩放大6倍。
从动带轮164直接固定在主轴130上，通过同步带162与主动带轮163连接；主动带轮163直接固定在旋转电机161上，通过旋转电机161的转动带动从动带轮164转动，进一步带动转盘110转动；
转盘驱动装置160可以为旋转电机161通过齿轮组与主轴130连接，驱动转盘110旋转；齿轮组包括：第一主动齿轮166、第一从动齿轮167，齿轮组的减速比为1：6；第一主动齿轮166固定在旋转电机161上，通过与第一从动齿轮167的啮合进而带动主轴130转动，进一步带动转盘110转动；
转盘驱动装置160也可以为旋转电机161通过联轴器165直接与主轴130连接，驱动转盘110转动。
主轴130的下侧固定着带有细长缺口的码盘121，支撑板300对应位置上固定有光耦122，当细长缺口转到光耦122中心处时，光耦接收端接收到光耦发射端发出的信号，该位置即为转盘110的初始位置；旋转电机161带动转盘110转动固定的角度，进一步带动混匀杯212到达设定的位置。
主轴130上固定有滑环150，该滑环150可于市场购得；主轴130为空心轴；滑环150转子导出的第一线束151通过主轴130内孔穿到转盘110上，与第一驱动电机211的控制电路板400连接，随着转盘110一起转动；滑环150的外壳即定子，通过挡销153限位固定不动，其导出的第二线束152固定不动，从而将转盘110内的线束引出外侧后，不会发生绕线现象。
本实施例中16个混匀机构200均布在转盘110外圈圆周上。当样本需要混匀时，相应的混匀机构200转动，带动样本进行混匀；
混匀方式包括两种：一种为第一驱动电机211直接驱动混匀杯212转动；另一种为第一驱动电机211通过第二主动齿轮213和第二从动齿轮214啮合传动带动混匀杯212转动。
第一驱动电机211直接驱动混匀杯212混匀是指混匀杯212通过螺丝直接固定在第一驱动电机211的驱动轴上；第一驱动电机211固定在转盘110的下侧，当第一驱动电机211转动时，带动混匀杯212旋转；试剂管220也可以装在混匀杯212上，当第一驱动电机211转动时，带动试剂管220转动混匀，在该实施例中最高混匀速度不超过1400r/min，并可以实现正反转。
第一驱动电机211也可以通过第二主动齿轮213与两个第二从动齿轮214啮合带动两个混匀杯212转动；第一驱动电机211固定在转盘110下侧，穿过转盘110与第二主动齿轮213连接；第二主动齿轮213外侧分布着两个第二从动齿轮214与之啮合，两个第二从动齿轮214各对应一个工位，与混匀杯212连接，分别带动混匀杯212转动。
16个第二从动齿轮214沿转盘110圆周均布在8个第二主动齿轮213的外侧；8个第二主动齿轮213由8个第一驱动电机211分别控制，第二主动齿轮213的转动带动两个第二从动齿轮214转动，第二从动齿轮214与第二主动齿轮213存在1：2的加速比，从而放大混匀杯211的转速，实现更好的混匀，在该实施例中，最高混匀速度不超过1400r/min，且由同一个第一驱动电机211控制的两个第二从动齿轮214转速相同，转向相反；
混匀杯212及第二从动齿轮214直接固定在小轴215上，位于转盘110的上侧；在转盘110的16个工位处皆安装有两个微型轴承216、217，在各工位处皆有小轴215穿过第一微型轴承216和第二微型轴承217内孔，与第二从动齿轮214连接，在第二从动齿轮214与第一微型轴承216之间装有有轴套218；第二从动齿轮214可以相对转盘110转动，带动混匀杯212内的生物聚合物混匀。
本实施例中的加样装置600在使用时可以实现加样针605的上下Z向移动，同时也可以绕Z轴旋转，所述加样装置600与带混匀功能的转盘旋转装置500配合，对试剂管220进行加样；其结构包括：加样针固定板601、导轨602、第二驱动电机603、第一丝杆604、加样针605；
具体地，本发明加样装置600与转盘旋转装置500配合对试剂管220进行加样，加样针固定板601上设有至少一个加样针605，当试剂管220需要加样时，转盘旋转机构500将需要加样的试剂管220通过旋转电机161转到加样针605的下方，加样装置600过第二驱动电机603驱动第一丝杆604，在导轨602的导向作用下带动加样针固定板601实现上下运动，进一步将加样针605深入试剂管220内，进行加样。进一步地，加样针固定板601上装有多个加样针605，通过从准备好的试剂瓶中吸取定量的试剂，分别可以对试剂管220加样不同液体。实际中，加样装置600也可以采用包括但不限于同步带形式，或通过龙门架机构实现该装置，以及其他能够实现加样功能的结构。
本实施例中的吸液装置700与带混匀功能的转盘旋转装置500配合，对试剂管220中的液体进行吸取；其结构包括：第三驱动电机701、第四驱动电机702、吸液针703、吸液臂704、第二丝杆705；
其中，吸液针703固定在吸液装置700上，吸液装置700也可提供两个自由度的运动，分别为通过第三驱动电机701通过驱动第二丝杆705从而带动吸液臂704旋转运动，第四驱动电机702通过驱动第二丝杆705从而带动吸液臂704轴向竖直上下移动；当试剂管220需要吸液时，转盘旋转装置500将需要排出废液的试剂管220通过旋转电机161转到预定的位置，第三驱动电机701将吸液针703旋转到试剂管220的上方，第四驱动电机702驱动吸液针703向试剂管220内移动，当吸液针703运动到设定位置时，在外接泵体的作用 下，将试剂管220内的液体吸出。完成废液吸取操作后，吸液装置700将吸液针703从试剂管220中移出，送入外部的清洗池中对吸液针703进行清洗。完成清洗后，吸液装置700将吸液针703从清洗池中移出，进行其他试剂管220的废液吸取处理，从而防止交叉感染。实际中，还可以采用通过带旋转及升降形式带动吸液针从其它位置进行吸液，以及通过其他运动机构实现对试剂管内液体进行吸液操作，所述运动机构包括但不限于机械臂、龙门架等形式。
本实施例中的磁吸装置800的结构包括：磁吸装置同步带802、第五驱动电机801、磁铁组件803、主同步轮804、从同步轮805；所述磁吸装置800可实现磁铁的径向运动，所述磁吸装置800与带混匀功能的转盘旋转装置500配合，将磁铁组件803置于需要磁吸的试剂管220外侧，对试剂管220中的磁珠进行磁吸操作；
具体地，当试剂管220内的磁珠需要侧磁吸时，转盘旋转装置500将需要磁吸的试剂管220通过旋转电机161转到与磁吸装置800的正对位置，第五驱动电机801与主同步轮804直接相连，所述主同步轮804通过磁吸装置同步带802带动从同步轮805转动，从而带动磁铁组件803前后运动，靠近或远离试剂管220，当将磁铁组件803靠近试剂管220外壁，将样品中的磁珠从试剂管220中吸出，如图16a所示；另一种是采用内磁吸方式，将磁铁组件803从试剂管220正上方伸入试剂管中，将样品中的磁珠从试剂管220中吸出，如图16b所示。进一步地，将磁珠磁吸出试剂管220后，吸液装置700带动吸液针703将试剂管220内的废液吸走。
本实施例中，进一步包括清洗装置1000，用于对吸液针703外壁进行清洗。具体地，清洗装置1000包括清洗池、第一隔膜泵、第二隔膜泵；其中，吸液针703移动到清洗池内，清洗池侧壁设有至少一个小孔，其通过管路与第一隔膜泵连接，用于喷出清洗液清洗吸液针703；第二隔膜泵用于将产生的冲洗液从清洗池抽走。实际中，如果吸液装置采用TIP头形式，则可以不设置清洗装置1000。清洗装置1000可以采用能达到相同功能的其他结构实现。
本实施例中的生物样本处理方法，包括以下步骤：
步骤1：将多个装有生物样本的试剂管分别插入混匀杯上；
步骤2：转盘旋转装置将试剂管转动到设定的加样位，加样装置将试剂加入试剂管内；
步骤3：启动转盘旋转装置的电机，正反转试剂管，将生物样本在试剂管内充分混匀；
步骤4：将完成混匀的生物样本通过转盘的转动到达设定的磁吸位，磁吸装置对试剂管内的磁珠吸附；
步骤5：完成吸附后，吸液装置将试剂管内的废液吸出；
步骤6：通过转盘的转动，可以连续处理多个生物样本。
本发明的保护内容不局限于以上实施例。在不背离本发明构思的精神和范围下，本领域技术人员能够想到的变化和优点都被包括在本发明中，并且以所附的权利要求书为保护范围。

Claims (8)

  1. 一种生物样本处理系统，其特征在于，所述系统包括：通过支撑结构(900)连接在一起的转盘旋转装置(500)和加样装置(600)、吸液装置(700)以及磁吸装置(800)中的一种或多种；其中，
    所述转盘旋转装置(500)包括：旋转转盘机构(100)和混匀机构(200)；所述旋转转盘机构(100)为可绕轴旋转的盘形结构，其通过转盘驱动装置(160)提供动力带动转盘(110)沿轴向旋转；所述混匀机构(200)包括：混匀驱动装置(210)和混匀杯(212)；一个或多个混匀机构(200)固定在所述转盘(110)上；混匀驱动装置(210)驱动混匀杯(212)沿轴向旋转运动；所述混匀杯(212)的轴向旋转和转盘(110)的轴向旋转相互独立；
    所述加样装置(600)与所述转盘旋转装置(500)配合，对试剂管(220)进行加样；
    所述吸液装置(700)与所述转盘旋转装置(500)配合，对试剂管(220)中的液体进行吸取；
    所述磁吸装置(800)与所述转盘旋转装置(500)配合，对试剂管(220)中的磁珠进行磁吸操作。
  2. 如权利要求1所述的生物样本处理系统，其特征在于，所述加样装置(600)包括：加样针固定板(601)、导轨(602)、第二驱动电机(603)、第一丝杆(604)、加样针(605)；
    其中，所述加样针固定板(601)上设有至少一根所述加样针(605)，给试剂管(220)加样，当所述试剂管(220)需要加样时，所述加样装置(600)通过第二驱动电机(603)驱动第一丝杆(604)，在导轨(602)的导向作用下带动加样针固定板(601)实现上下运动，进一步将加样针(605)深入试剂管(220)内，进行加样。
  3. 如权利要求1所述的生物样本处理系统，其特征在于，所述吸液装置(700)包括：第三驱动电机(701)、第四驱动电机(702)、吸液针(703)、吸液臂(704)、第二丝杆(705)；
    其中，所述吸液针(703)固定在所述吸液装置(700)上，所述吸液装置(700)通过所述第三驱动电机(701)驱动第二丝杆(705)带动吸液臂(704)转动；所述吸液装置(700)通过所述第四驱动电机(702)驱动第二丝杆(705)带动吸液臂(704)轴向竖直上下移动；当所述试剂管(220)需要吸液时，所述第三驱动电机(701)驱动第二丝杆(705)带动吸液臂(704)将所述吸液针(703)旋转到所述试剂管(220)的上方，所述第四驱动电机(702)驱动第二丝杆(705)带动吸液臂(704)带动所述吸液针(703)向试剂管(220)内移动，当吸液针(703)运动到设定位置时，将试剂管(220)内的液体吸出。
  4. 如权利要求1所述的生物样本处理系统，其特征在于，所述磁吸装置(800)包括：磁吸装置同步带(802)、第五驱动电机(801)、磁铁组件(803)、主同步轮(804)、从同 步轮(805)；
    其中，所述第五驱动电机(801)与主同步轮(804)直接相连，所述主同步轮(804)通过所述磁吸装置同步带(802)带动从同步轮(805)转动，从而带动磁铁组件(803)运动，靠近或者远离试剂管(220)，对生物样品中的磁珠进行磁吸。
  5. 如权利要求1所述的生物样本处理系统，其特征在于，所述旋转转盘机构(100)包括：转盘(110)、主轴(130)、轴承座(140)、轴承(141、142)、转盘驱动装置(160)、支撑板(300)；
    所述主轴(130)与转盘(110)连接，主轴(130)穿过轴承(141、142)内孔，通过轴承座(140)安装在支撑板(300)上；
    所述转盘驱动装置(160)进一步包括：旋转电机(161)和传动机构；
    所述传动机构包括：同步带(162)及同步带轮(163、164)，或联轴器(165)，或齿轮组(166、167)；
    所述旋转电机(161)通过联轴器(165)直接与主轴(130)连接，驱动转盘(110)旋转；或通过同步带轮(163、164)、同步带(162)与主轴(130)连接，驱动转盘(110)旋转；或通过齿轮组(166、167)与主轴(130)连接，驱动转盘(110)旋转。
  6. 如权利要求1所述的生物样本处理系统，其特征在于，所述混匀杯(212)直接固定在第一驱动电机(211)的驱动轴上，带动混匀杯(212)旋转；和/或，所述第一驱动电机(211)与第二主动齿轮(213)连接，通过第二主动齿轮(213)啮合带动第二从动齿轮(214)转动，从而带动混匀杯(212)旋转；
    所述第一驱动电机(211)固定在所述转盘(110)的圆周上；
    所述第二主动齿轮(213)外侧分布着第二从动齿轮(214)与之啮合；
    所述混匀杯(212)旋转时，带动安装在混匀杯(212)上的试剂管(220)内的生物聚合物混匀；和/或，所述混匀杯(212)旋转时，带动直接加入混匀杯(212)内的生物聚合物混匀。
  7. 如权利要求6所述的生物样本处理系统，其特征在于，所述混匀杯(212)和第二从动齿轮(214)固定在小轴(215)上；
    所述小轴(215)在转盘(110)的相应工位处安装有两个微型轴承(216、217)，所述小轴(215)穿过所述微型轴承(216、217)内孔间接地与转盘(110)连接，从而实现从动齿轮(214)相对转盘(110)圆周转动。
  8. 一种生物样本处理方法，其特征在于，其采用如权利要求1-7中任一项所述的处理系统，所述方法包括以下步骤：
    步骤1：将多个装有生物样本的试剂管(220)分别插入混匀杯上；
    步骤2：转盘旋转装置(500)将试剂管(220)转动到设定的加样位，加样装置(600)将试剂加入试剂管(220)内；
    步骤3：启动转盘旋转装置的电机，正反转试剂管(220)，将生物样本在试剂管(220)内充分混匀；
    步骤4：将完成混匀的生物样本通过转盘(110)的转动到达设定的磁吸位，磁吸装置(800)对试剂管(220)内的磁珠进行吸附；
    步骤5：完成吸附后，吸液装置(700)将试剂管(220)内的废液吸出；
    步骤6：通过转盘(110)的转动，可以连续处理多个生物样本。
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