WO2022143577A1 - 一种带有混匀功能工位的转盘旋转装置及其使用方法 - Google Patents

一种带有混匀功能工位的转盘旋转装置及其使用方法 Download PDF

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WO2022143577A1
WO2022143577A1 PCT/CN2021/141833 CN2021141833W WO2022143577A1 WO 2022143577 A1 WO2022143577 A1 WO 2022143577A1 CN 2021141833 W CN2021141833 W CN 2021141833W WO 2022143577 A1 WO2022143577 A1 WO 2022143577A1
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Prior art keywords
turntable
mixing
rotate
mixing cup
rotating
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PCT/CN2021/141833
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English (en)
French (fr)
Inventor
张昊
孙振波
房瑜
魏金文
张准
乔月华
王林
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南京金斯瑞生物科技有限公司
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Publication of WO2022143577A1 publication Critical patent/WO2022143577A1/zh

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F29/00—Mixers with rotating receptacles
    • B01F29/30—Mixing the contents of individual packages or containers, e.g. by rotating tins or bottles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/10—Mixers with shaking, oscillating, or vibrating mechanisms with a mixing receptacle rotating alternately in opposite directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/20—Mixing the contents of independent containers, e.g. test tubes
    • B01F31/23—Mixing the contents of independent containers, e.g. test tubes by pivoting the containers about an axis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/80—Mixing plants; Combinations of mixers
    • B01F33/81—Combinations of similar mixers, e.g. with rotary stirring devices in two or more receptacles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/80—Mixing plants; Combinations of mixers
    • B01F33/85—Mixing plants with mixing receptacles or mixing tools that can be indexed into different working positions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
    • B01F2101/23—Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
    • B01F2101/44—Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation

Definitions

  • the invention belongs to the technical field of biological sample processing, and relates to a biopolymer mixing device, in particular to a plasmid extraction or protein purification device, and in particular to a turntable rotating device with a mixing function station and a method of using the same.
  • Traditional operation method 1 Set the mixing position separately for mixing. When other operations are required, the samples need to be transferred to other stations for further operations. After the operation is complete, if you need to continue mixing, you need to put the sample back on the mixing position. Therefore, the operation process is cumbersome, the instrument is not compact enough, and mixing and other operations cannot be performed at the same time.
  • the second method of traditional operation is to mix the sample by continuously sucking and stirring the sample with the sample needle. This method also cannot satisfy mixing and other operations such as sample addition, cleaning and transfer at the same time, and the structure is complicated.
  • the present invention provides a turntable rotating device with a mixing function station. mixing station.
  • a mixing cup can be placed on each mixing station to drive the reagent tube to rotate and mix.
  • the device of the invention combines the functions of the rotating turntable and the mixing, which can greatly simplify the operation process and the instrument. Since the rotation of the turntable and the mixing function are independent of each other, they can be performed at the same time. Therefore, the device rotates the reagent tubes to different positions in the circumference according to the program settings. With the cooperation of the extraction module, automatic sample addition, automatic cleaning, automatic transfer and other functions can be performed at the same time as the automatic mixing function without affecting each other.
  • the invention provides a turntable rotating device with a mixing function station, comprising: a rotating turntable mechanism and a mixing mechanism;
  • the rotating turntable mechanism mainly includes a turntable, a main shaft, a bearing seat, a bearing, a support plate, a turntable driving device, a position sensor and the like.
  • the main shaft is connected with the turntable, and the main shaft passes through the inner hole of the bearing and is mounted on the support plate through the bearing seat.
  • the upper and lower ends of the bearing seat are provided with a first bearing and a second bearing; the outer rings of the first bearing and the second bearing are respectively connected with the bearing seat; the main shaft passes through the first bearing and the second bearing. the inner ring of the second bearing.
  • the turntable driving device drives the turntable to realize circular rotation, and judges the rotation angle of the turntable through the position sensor.
  • the mixing mechanism is fixed on the turntable, and the number can be set to one or more as required, and is distributed along the circumference of the turntable to provide a plurality of rotating mixing stations.
  • the mixing mechanism mainly includes a mixing cup and a mixing driving device.
  • the mixing drive device drives the mixing cup to rotate along the axial direction.
  • the mixing cup rotates, the biopolymer in the reagent tube installed on the mixing cup is driven to mix; and/or, the biopolymer directly added into the mixing cup is driven to mix.
  • the turntable driving device may be a rotary motor that drives the turntable to rotate through a synchronous pulley set;
  • the synchronous pulley set includes: a driving pulley, a driven pulley, and a synchronous belt; wherein, the driven pulley is fixed on the on the main shaft; the driven pulley is connected to the driving pulley through the synchronous belt; the driving pulley is fixed on the rotating motor, and the driven pulley is driven to rotate by the rotation of the rotating motor, and further drive the turntable to rotate;
  • the turntable driving device can be a rotary motor that directly drives the main shaft to rotate; the rotary motor is directly connected to the main shaft through a coupling to drive the turntable to rotate;
  • the turntable driving device may be a rotary motor to drive the turntable to rotate through a gear set; the gear set includes: a first driving gear and a first driven gear.
  • the rotating electrical machine is connected with the first driving gear, and the first driven gear is connected with the main shaft; the first driving gear is meshed with the first driven gear, and the first driving gear is with the first driven gear.
  • the rotary motion of the rotary motor is directly transmitted through the tooth meshing, which further drives the turntable to rotate.
  • the rotating turntable mechanism is optionally equipped with a position sensor, and the function of the position sensor can be realized by a code disk and an optocoupler;
  • the code disk is a circular sheet with an elongated notch and is fixed on the main shaft;
  • the optocoupler is fixed On the support plate, the initial position of the turntable is confirmed by whether the code disc blocks the optical signal of the optocoupler in the process of rotating with the main shaft, and the position of each mixing station on the turntable is controlled by controlling the rotation steps of the rotating motor. .
  • the optional structure of the rotating turntable mechanism includes a slip ring; the slip ring can be purchased in the market; the slip ring is connected with the main shaft; the main shaft is a hollow shaft; the slip ring includes a rotor and a stator, and the rotor During the rotation process, it can still transmit electrical signals with the stator;
  • the first wire harness derived from the slip ring rotor passes through the inner hole of the main shaft to the turntable and is connected to the control circuit board of the drive motor; the outer casing of the slip ring is the stator, which is fixed and fixed by the stopper pin, and can be Lead out the fixed second wire harness.
  • the mixing cup can be directly fixed on the drive shaft of the driving motor, and the driving motor is fixed on the lower side of the turntable.
  • the driving motor rotates, the mixing cup is driven to rotate; and/or, the The driving motor is connected with the second driving gear, and drives the second driven gear to rotate through the meshing of the second driving gear, thereby driving the mixing cup to rotate;
  • each second driven gear is provided with a mixing cup
  • the mixing cup and the second driven gear are fixed on the small shaft; the small shaft is installed with two miniature bearings at the corresponding stations of the turntable, which pass through the inner hole of the miniature bearings and are indirectly connected to the turntable, Thereby, the second driven gear rotates relative to the circumference of the turntable.
  • the present invention also proposes a method for using a turntable rotating device with a mixing function station, comprising the following steps:
  • Step 1 Insert the reagent tube containing the biological sample into the mixing cup or directly add the biological sample into the mixing cup;
  • Step 2 Start the drive motor, control the speed and direction of each drive motor individually according to the actual process requirements, and set the mixing cup forward and reverse;
  • Step 3 Move the mixed biological sample to the set sample processing position through the rotation of the turntable, and proceed to the next step;
  • Step 4 Through the rotation of the turntable, multiple biological samples can be processed continuously.
  • the present invention combines the mixing function with the rotation of the turntable, and at the same time of mixing, the turntable rotates the sample to be processed to the set position, and performs operations such as sample addition, cleaning, extraction, etc. .
  • the existing processing method it has the advantages of simple and compact structure, and can process multiple reagent tubes at the same time, and operations such as sample addition, cleaning, and extraction can be performed simultaneously with mixing without affecting each other, saving operation time.
  • Figure 1 is a schematic diagram of the overall structure of the present invention.
  • FIG. 2 is a schematic diagram of the overall structure of the present invention from a first perspective.
  • FIG. 3 is a schematic diagram of the overall structure of the present invention from a second perspective.
  • FIG. 4 is a schematic diagram of the rotary motor directly driving the turntable to rotate according to the present invention.
  • FIG. 5 is a schematic diagram of the rotary motor of the present invention driving the turntable to rotate through the gear set.
  • Fig. 6 is a sectional view of the rotation of the main shaft of the present invention.
  • FIG. 7 is a schematic diagram of the lead wire harness of the slip ring of the present invention.
  • FIG. 8 is a schematic diagram of the mixing mode of the gear drive driving the mixing cup of the present invention.
  • FIG. 9 is a schematic structural diagram of the mixing cup relative to the turntable in the mixing mode of the gear drive driving the mixing cup according to the present invention.
  • FIG. 10 is a schematic diagram of the mixing method of the motor directly driving the mixing cup according to the present invention.
  • 100-Rotary turntable mechanism 110-Turntable, 161-Rotary motor, 121-Code disc, 122-Optocoupler, 162-Synchronous belt, 163-Active pulley, 164-Driven pulley, 153-Block pin, 165- Coupling, 130-main shaft, 167-first driven gear, 166-first driving gear, 140-bearing seat, 141-first bearing, 142-second bearing, 150-slip ring, 151-first wire harness , 152-second wiring harness, 160-turntable drive device;
  • the turntable rotating device of the present invention includes two major mechanisms: a rotating turntable mechanism 100 and a mixing mechanism 200 .
  • the rotating turntable mechanism 100 includes: a turntable 110 , a main shaft 130 , a bearing seat 140 , bearings 141 and 142 , a turntable driving device 160 , a support plate 300 , and a position sensor.
  • the rotary turntable mechanism 100 is a disc-shaped structure that can rotate around an axis. There are 16 workstations distributed along the outer circumference of the disc, and 16 samples can be stored. The samples can be transferred to preset positions according to process requirements.
  • the bearing seat 140 is fixed on the upper side of the support plate 300.
  • the upper and lower ends of the bearing seat 140 are respectively equipped with a first bearing 141 and a second bearing 142.
  • the outer rings of the first bearing 141 and the second bearing 142 are respectively connected with the bearing seat 140;
  • the main shaft 130 passes through the inner rings of the first bearing 141 and the second bearing 142 , the upper side of the main shaft 130 is connected to the turntable 110 , and the lower side of the main shaft 130 is connected to the turntable driving device 160 ;
  • the turntable driving device 160 may be a rotary motor 161 connected to the main shaft 130 through a synchronous pulley set to drive the turntable 110 to rotate;
  • the synchronous pulley set includes: a driving pulley 163, a driven pulley 164, a synchronous belt 162, and a synchronous pulley 163.
  • the pulley set has a reduction ratio of 1:6 for reducing the rotational speed of the turntable 110 and amplifying the output torque of the rotary motor 161 by 6 times.
  • the driven pulley 164 is directly fixed on the main shaft 130, and is connected with the driving pulley 163 through the synchronous belt 162; the driving pulley 163 is directly fixed on the rotary motor 161, and the rotation of the rotary motor 161 drives the The driven pulley 164 rotates, which further drives the turntable 110 to rotate;
  • the turntable driving device 160 may be a rotary motor 161 connected to the main shaft 130 through a gear set to drive the turntable 110 to rotate;
  • the gear set includes: a first driving gear 166 and a first driven gear 167, and the reduction ratio of the gear set is 1 : 6, the first driving gear 166 is fixed on the rotating motor 161, and then drives the main shaft 130 to rotate through the meshing with the first driven gear 167, and further drives the turntable 110 to rotate;
  • the turntable driving device 160 may also be a rotary motor 161 directly connected to the main shaft 130 through a coupling 165 to drive the turntable 110 to rotate.
  • the code disc 121 with an elongated notch is fixed on the lower side of the main shaft 130, and the optical coupler 122 is fixed on the corresponding position of the support plate 300.
  • the optical coupler receiving end After receiving the signal from the optocoupler transmitter, this position is the initial position of the turntable 110; the rotary motor 161 drives the turntable 110 to rotate by a fixed angle, and further drives the mixing cup 212 to reach the set position.
  • a slip ring 150 is fixed on the main shaft 130, and the slip ring 150 can be purchased in the market;
  • the main shaft 130 is a hollow shaft;
  • the first wire harness 151 derived from the rotor of the slip ring 150 passes through the inner hole of the main shaft 130 to the turntable 110 connected to the control circuit board 400 of the drive motor 211, and rotates with the turntable 110;
  • the casing of the slip ring 150 is the stator, which is limited and fixed by the stopper pin 153, and the second wire harness 152 derived from it is fixed. , so that after the wire harness in the turntable 110 is drawn out to the outside, the winding phenomenon will not occur.
  • the 16 mixing mechanisms 200 are evenly distributed on the circumference of the outer ring of the turntable 110 .
  • the corresponding mixing mechanism 200 rotates to drive the sample to mix;
  • the mixing methods include two: one is that the driving motor 211 directly drives the mixing cup 212 to rotate; the other is that the driving motor 211 drives the mixing cup 212 through the meshing transmission of the second driving gear 213 and the second driven gear 214. turn.
  • the driving motor 211 directly drives the mixing cup 212 for mixing, which means that the mixing cup 212 is directly fixed on the driving shaft of the driving motor 211 by screws; When the 211 rotates, it drives the mixing cup 212 to rotate; the reagent tube 220 can also be installed on the mixing cup 212. When the driving motor 211 rotates, it drives the reagent tube 220 to rotate and mix evenly.
  • the maximum mixing speed does not exceed 1400r/min, and can realize forward and reverse.
  • the driving motor 211 can also drive the two mixing cups 212 to rotate through the meshing of the second driving gear 213 and the two second driven gears 214; the driving motor 211 is fixed on the lower side of the turntable 110 and passes through the turntable 110 and The second driving gear 213 is connected; two second driven gears 214 are distributed on both sides of the second driving gear 213 to mesh with it. , respectively drive the mixing cup 212 to rotate.
  • the 16 second driven gears 214 are evenly distributed on both sides of the 8 second driving gears 213 along the circumference of the turntable 110 ; the 8 second driving gears 213 are controlled by the 8 driving motors 211 respectively.
  • the rotation drives the two second driven gears 214 to rotate, and the second driven gear 214 and the second driving gear 213 have an acceleration ratio of 1:2, thereby amplifying the rotation speed of the mixing cup 211 and achieving better mixing.
  • the maximum mixing speed does not exceed 1400 r/min, and the two second driven gears 214 controlled by the same driving motor 211 have the same rotational speed and opposite directions;
  • the mixing cup 212 and the second driven gear 214 are directly fixed on the small shaft 215 and are located on the upper side of the turntable 110 ; two miniature bearings 216 and 217 are installed at the 16 stations of the turntable 110 . There are small shafts 215 at the workstations that pass through the inner holes of the first miniature bearing 216 and the second miniature bearing 217 , and are connected to the second driven gear 214 . There is a shaft sleeve 218;
  • the second driven gear 214 can rotate relative to the turntable 110 to drive the biopolymer in the mixing cup 212 to mix.
  • the use process of the device is specifically described by taking the resuspension process in the plasmid extraction process as an example:
  • Step 1 Insert a plurality of reagent tubes 220 containing 10ml of bacterial slurry into the upper mixing cup 212 respectively;
  • Step 2 Start the drive motor 211, set the mixing speed to 1400r/min, forward 0.5s, reverse 0.5s, and the intermediate switching time is 0.2s, and the total mixing time is 10min; at this time, the bacterial slurry is fully mixed in the reagent tube 220 Evenly, there is no attachment on the wall surface, no floccules in the solution, and the whole is a uniform milky white liquid;
  • Step 3 The mixed biological sample reaches the set sample processing position through the rotation of the turntable 110, and the next step is performed;
  • Step 4 Through the rotation of the turntable 110, a plurality of biological samples can be processed continuously.

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Abstract

一种带有混匀功能工位的转盘旋转装置及使用方法，该装置为轴向转动的转盘（110），在转盘（110）上沿圆周分布着一个或者多个轴向转动的混匀工位。每个混匀工位上均可以放置混匀杯（212），带动试剂管（220）旋转混匀。旋转的转盘（110）带动试剂管（220）按程序设定转到圆周内不同的位置，在相应位置上可以与其它加样、提取、清洗装置配合实现自动加样、提取、清洗等功能。

Description

一种带有混匀功能工位的转盘旋转装置及其使用方法
本申请要求申请日为2020年12月28日、申请号为202011579905.0、发明名称为“一种带有混匀功能工位的转盘旋转装置及其使用方法”的中国发明专利申请的优先权。
技术领域
本发明属于生物样本处理技术领域，涉及生物聚合物混匀装置，更具体的为质粒提取或蛋白纯化装置，尤其涉及一种带有混匀功能工位的转盘旋转装置及其使用方法。
背景技术
在现代生物工程中，一般需要加入试剂，并进行混匀，以达到物质充分反应的目的。而混匀后的物质需要进行加样、清洗、转移等操作，而且经常会被要求与混匀同时进行。
传统的操作方法一：单独设置混匀位进行混匀。在需要其它操作时，需要将样本转移到其它工位进行下一步操作。操作完成后，如果需要继续混匀，则需要将样本再放回混匀位上。因此操作工艺繁琐，仪器不够紧凑，且混匀与其它操作不能同时进行。
传统的操作方法二：通过加样针不断地吸打样本进行混匀。该方法同样不能满足混匀与其它加样、清洗、转移等操作同时进行,且结构复杂。
发明内容
为了解决上述方法上的不足，本发明提供了一种带有混匀功能工位的转盘旋转装置，该装置为轴向转动的大转盘，在转盘上沿圆周分布着一个或者多个轴向转动的混匀工位。每个混匀工位上均可以放置混匀杯，带动试剂管旋转混匀。本发明装置将旋转转盘和混匀功能结合在一起，可极大地简化操作流程和仪器。由于转盘旋转与混匀功能相互独立，互不影响，可以同时进行，因此该装置将试剂管按程序设定转到圆周内不同的位置，在相应位置上可以与其它加样模块、清洗模块、提取模块配合，可实现自动加样，自动清洗，自动转移等功能与自动混匀功能同时进行，互不影响。
本发明提出了一种带有混匀功能工位的转盘旋转装置，包括：旋转转盘机构和混匀机构；
所述旋转转盘机构主要包括：转盘、主轴、轴承座、轴承、支撑板、转盘驱动装置，位置传感器等组成。
所述主轴与转盘连接，主轴穿过轴承内孔，通过轴承座安装在支撑板上。
所述轴承座上下两端设有第一轴承和第二轴承；所述第一轴承和所述第二轴承的外圈分别与所述轴承座连接；所述主轴穿过所述第一轴承和所述第二轴承的内圈。
所述转盘驱动装置带动转盘实现圆周转动，并通过位置传感器判断转盘的旋转角度。
所述混匀机构固定在转盘上，数量根据需要可设置为一个或多个，并沿所述转盘的圆周分布，提供多个旋转混匀工位。
所述混匀机构主要包括混匀杯和混匀驱动装置。混匀驱动装置驱动混匀杯沿轴向旋转运动。
所述混匀杯旋转时，带动安装在混匀杯上的试剂管内的生物聚合物混匀；和/或，带动直接加入混匀杯内的生物聚合物混匀。
所述转盘驱动装置可以为旋转电机通过同步带轮组驱动转盘旋转；所述同步带轮组包括：主动带轮、从动带轮、同步带；其中，所述从动带轮固定在所述主轴上；所述从动带轮通过所述同步带与所述主动带轮连接；所述主动带轮固定在旋转电机上，通过所述旋转电机的转动带动所述从动带轮转动，进一步带动转盘转动；
所述转盘驱动装置可以为旋转电机直接驱动主轴旋转；所述旋转电机通过联轴器与主轴直接连接，驱动转盘转动；
所述转盘驱动装置可以为旋转电机通过齿轮组驱动转盘旋转；所述齿轮组包括：第一主动齿轮、第一从动齿轮。所述旋转电机与所述第一主动齿轮连接，所述第一从动齿轮与所述主轴连接；所述第一主动齿轮与第一从动齿轮啮合，第一主动齿轮与第一从动齿轮直接通过齿啮合传递旋转电机的旋转运动，进一步带动转盘转动。
所述旋转转盘机构可选的装有位置传感器，位置传感器的功能可通过码盘和光耦实现；所述码盘为圆形薄片并设有细长缺口，固定在主轴上；所述光耦固定在所述支撑板上，通过码盘在随着主轴转动过程中是否遮挡光耦的光信号来确认转盘的初始位置，通过控制旋转电机的转动步数，控制转盘上各混匀工位的位置。
所述旋转转盘机构可选的结构包括滑环；所述滑环可于市场购得；所述滑环与主轴连接；所述主轴为空心轴；所述滑环包括转子和定子两部分，转子转动过程中，仍能与定子相互传递电信号；
所述滑环转子导出的第一线束通过所述主轴内孔穿到转盘上，与驱动电机的控制电路板连接；所述滑环的外壳即定子，其通过挡销限位固定不动，可引出固定不动的第二线束。
所述混匀杯可以直接固定在驱动电机的驱动轴上，所述驱动电机固定在所述的转盘的下侧，当所述驱动电机转动时，带动混匀杯旋转；和/或，所述驱动电机与所述第二主动齿轮连接，通过第二主动齿轮啮合带动第二从动齿轮转动，从而带动混匀杯旋转；
所述第二主动齿轮外侧分布着两个第二从动齿轮与之啮合；每个第二从动齿轮上装有一个混匀杯；
所述混匀杯和第二从动齿轮固定在小轴上；所述小轴在转盘的相应工位处安装有两个 微型轴承，穿过所述微型轴承内孔，间接地与转盘连接，从而实现第二从动齿轮相对转盘圆周转动。
基于以上装置，本发明还提出了一种带有混匀功能工位的转盘旋转装置的使用方法，包括以下步骤：
步骤1：将装有生物样本的试剂管插入混匀杯或者将生物样本直接加入混匀杯内；
步骤2：启动驱动电机，根据实际工艺要求单独控制每个驱动电机的转速和方向，并可以设置混匀杯正反转；
步骤3：将完成混匀的生物样本通过转盘的转动到达设定的样本处理位，进行下一步操作；
步骤4：通过转盘的转动，可以连续处理多个生物样本。
本发明的有益效果在于：本发明将混匀功能与转盘旋转结合在一起，在混匀的同时，转盘通过旋转将需要处理的样本转到设定的位置，进行加样、清洗、提取等操作。与现有的处理方法相比，具有结构简单紧凑，可以同时处理多个试剂管的优点，而且加样、清洗、提取等操作可以与混匀同时进行，互不影响，节省操作时间。
附图说明
图1是本发明整体结构简图。
图2是本发明整体结构第一视角示意图。
图3是本发明整体结构第二视角示意图。
图4为本发明旋转电机直接驱动转盘转动的示意图。
图5为本发明旋转电机通过齿轮组驱动转盘转动的示意图。
图6是本发明主轴旋转的剖视图。
图7是本发明滑环引出线束的示意图。
图8是本发明齿轮传动驱动混匀杯混匀方式的示意图。
图9是本发明齿轮传动驱动混匀杯混匀方式的混匀杯相对转盘的结构示意图。
图10是本发明电机直接驱动混匀杯混匀方式的示意图。
图1-10中：
100-旋转转盘机构、110-转盘、161-旋转电机、121-码盘、122-光耦、162-同步带、163-主动带轮、164-从动带轮、153-挡销、165-联轴器、130-主轴、167-第一从动齿轮、166-第一主动齿轮、140-轴承座、141-第一轴承、142-第二轴承、150-滑环、151-第一线束、152-第二线束、160-转盘驱动装置；
200-混匀机构、212-混匀杯、214-第二从动齿轮、213-第二主动齿轮、211-驱动电机、 215-小轴、216-第一微型轴承、217-第二微型轴承、218-轴套、210-混匀驱动装置；
300-支撑板；
220-试剂管；
400-控制电路板。
具体实施方式
结合以下具体实施例和附图，对发明作进一步的详细说明。实施本发明的过程、条件、实验方法等，除以下专门提及的内容之外，均为本领域的普遍知识和公知常识，本发明没有特别限制内容。
如图1-图10所示，在本实施例中，本发明转盘旋转装置包括两大机构：旋转转盘机构100和混匀机构200。
本实施例中旋转转盘机构100包括：转盘110、主轴130、轴承座140、轴承141、142、转盘驱动装置160、支撑板300、位置传感器。
所述旋转转盘机构100为可绕轴旋转的盘形结构，沿圆盘外侧圆周一圈分布16个工位，可存放16个样本，样本可根据工艺需求转到预设的位置。
所述轴承座140固定在支撑板300的上侧，轴承座140上下两端各装有第一轴承141和第二轴承142，所述第一轴承141和所述第二轴承142的外圈分别与轴承座140连接；
所述主轴130穿过第一轴承141和第二轴承142的内圈，主轴130上侧与所述转盘110连接，主轴130下侧与转盘驱动装置160连接；进而转盘110相对支撑板300只有绕轴旋转的一个自由度。
所述转盘驱动装置160可以为旋转电机161通过同步带轮组与主轴130连接，驱动转盘110旋转；所述同步带轮组包括：主动带轮163、从动带轮164、同步带162，同步带轮组存在1:6的减速比用于降低转盘110的转速，并将旋转电机161的输出转矩放大6倍。
所述从动带轮164直接固定在主轴130上，通过同步带162与主动带轮163连接；所述主动带轮163直接固定在旋转电机161上，通过所述旋转电机161的转动带动所述从动带轮164转动，进一步带动转盘110转动；
所述转盘驱动装置160可以为旋转电机161通过齿轮组与主轴130连接，驱动转盘110旋转；所述齿轮组包括：第一主动齿轮166、第一从动齿轮167，齿轮组的减速比为1：6，第一主动齿轮166固定在旋转电机161上，通过与第一从动齿轮167的啮合进而带动主轴130转动，进一步带动转盘110转动；
所述转盘驱动装置160也可以为旋转电机161通过联轴器165直接与主轴130连接，驱动转盘110转动。
所述主轴130的下侧固定着带有细长缺口的码盘121，所述支撑板300对应位置上固定有光耦122，当细长缺口转到光耦122中心处时，光耦接收端接收到光耦发射端发出的信号，该位置即为转盘110的初始位置；旋转电机161带动转盘110转动固定的角度，进一步带动混匀杯212到达设定的位置。
所述主轴130上固定有滑环150，该滑环150可于市场购得；所述主轴130为空心轴；所述滑环150转子导出的第一线束151通过主轴130内孔穿到转盘110上，与驱动电机211的控制电路板400连接，随着转盘110一起转动；所述滑环150的外壳即定子，通过挡销153限位固定不动，其导出的第二线束152固定不动，从而将转盘110内的线束引出外侧后，不会发生绕线现象。
本实施例中16个混匀机构200均布在转盘110外圈圆周上。当样本需要混匀时，相应的混匀机构200转动，带动样本进行混匀；
所述混匀方式包括两种：一种为驱动电机211直接驱动混匀杯212转动；另一种为驱动电机211通过第二主动齿轮213和第二从动齿轮214啮合传动带动混匀杯212转动。
所述驱动电机211直接驱动混匀杯212混匀是指混匀杯212通过螺丝直接固定在驱动电机211的驱动轴上；所述驱动电机211固定在转盘110的下侧，当所述驱动电机211转动时，带动混匀杯212旋转；试剂管220也可以装在混匀杯212上，当驱动电机211转动时，带动试剂管220转动混匀，在该实施例中最高混匀速度不超过1400r/min，并可以实现正反转。
所述驱动电机211也可以通过第二主动齿轮213与两个第二从动齿轮214啮合带动两个混匀杯212转动；所述驱动电机211固定在转盘110的下侧，穿过转盘110与第二主动齿轮213连接；所述第二主动齿轮213两侧分布着两个第二从动齿轮214与之啮合，两个第二从动齿轮214各对应一个工位，与混匀杯212连接，分别带动混匀杯212转动。
16个第二从动齿轮214沿转盘110圆周均布在8个第二主动齿轮213的两侧；所述8个第二主动齿轮213由8个驱动电机211分别控制，第二主动齿轮213的转动带动两个第二从动齿轮214转动，第二从动齿轮214与第二主动齿轮213存在1：2的加速比，从而放大混匀杯211的转速，实现更好的混匀，在该实施例中，最高混匀速度不超过1400r/min，且由同一个驱动电机211控制的两个第二从动齿轮214转速相同，转向相反；
所述混匀杯212及第二从动齿轮214直接固定在小轴215上，位于转盘110的上侧；在转盘110的16个工位处皆安装有两个微型轴承216、217，在各工位处皆有小轴215穿过第一微型轴承216和第二微型轴承217内孔，与第二从动齿轮214连接，在第二从动齿轮214与第一微型轴承216之间装有有轴套218；
所述第二从动齿轮214可以相对转盘110转动，带动混匀杯212内的生物聚合物混匀。
在本实施例中，以质粒提取过程中的重悬工艺为例具体说明装置的使用过程：
步骤1：将多个装有10ml菌泥的试剂管220分别插入混匀杯212上中；
步骤2：启动驱动电机211，设定混匀转速1400r/min，正传0.5s，反转0.5s中间切换时间0.2s，总计混匀时间10min；这时，菌泥在试剂管220内充分混匀，壁面无附着物，溶液内无絮状物，整体呈均匀的乳白色液体；
步骤3：将完成混匀的生物样本通过转盘110的转动到达设定的样本处理位，进行下一步操作；
步骤4：通过转盘110的转动，可以连续处理多个生物样本。
本发明的保护内容不局限于以上实施例。在不背离发明构思的精神和范围下，本领域技术人员能够想到的变化和优点都被包括在本发明中，并且以所附的权利要求书为保护范围。

Claims (10)

  1. 一种带有混匀功能工位的转盘旋转装置，其特征在于，将试剂混匀功能集成到转盘(110)上；其结构包括：旋转转盘机构(100)和混匀机构(200)；
    所述旋转转盘机构(100)为可绕轴旋转的盘形结构，其通过转盘驱动装置(160)提供动力带动转盘(110)沿轴向旋转；
    所述混匀机构(200)包括：混匀驱动装置(210)和混匀杯(212)；一个或多个混匀机构(200)固定在所述转盘(110)上；混匀驱动装置(210)驱动混匀杯(212)沿轴向旋转运动；
    所述混匀杯(212)的轴向旋转和转盘(110)的轴向旋转相互独立。
  2. 如权利要求1所述的转盘旋转装置，其特征在于，所述旋转转盘机构(100)包括：转盘(110)、主轴(130)、轴承座(140)、轴承(141、142)、转盘驱动装置(160)、支撑板(300)。
  3. 如权利要求2所述的转盘旋转装置，其特征在于，旋转转盘机构(100)中，所述主轴(130)与转盘(110)连接，主轴(130)穿过轴承(141、142)内孔，通过轴承座(140)安装在支撑板(300)上；
    所述转盘驱动装置(160)进一步包括：旋转电机(161)和传动机构；
    所述传动机构包括：同步带(162)及同步带轮(163、164)，或联轴器(165)，或齿轮组(166、167)。
  4. 如权利要求3所述的转盘旋转装置，其特征在于，所述旋转电机(161)通过联轴器(165)直接与主轴(130)连接，驱动转盘(110)旋转；或通过同步带轮(163、164)、同步带(162)与主轴(130)连接，驱动转盘(110)旋转；或通过齿轮组(166、167)与主轴(130)连接，驱动转盘(110)旋转。
  5. 如权利要求1所述的转盘旋转装置，其特征在于，所述旋转转盘机构(100)配置滑环(150)；
    所述滑环(150)用于解决将线束(151、152)引入转盘(110)后，随着转盘(110)的转动发生的绕线问题。
  6. 如权利要求1所述的转盘旋转装置，其特征在于，所述旋转转盘机构(100)配置位置传感器，用于判断各工位所在的位置。
  7. 如权利要求6所述的转盘旋转装置，其特征在于，所述位置传感器包括码盘(121)和光耦(122)；所述码盘(121)固定在主轴(130)上，与主轴(130)同步旋转运动；
    所述码盘(121)设有细长狭缝，当缺口转到光耦(122)位置处时，光耦(122)发射端发射的光信号穿过细长狭缝，被光耦(122)的接收端接受，确定转盘(110)的初始位置； 通过软件控制，使转盘(110)转动固定的角度，进一步带动混匀杯(212)到达设定的位置。
  8. 如权利要求1所述的转盘旋转装置，其特征在于，所述混匀杯(212)直接固定在驱动电机(211)的驱动轴上，带动混匀杯(212)旋转；和/或，所述驱动电机(211)与第二主动齿轮(213)连接，通过第二主动齿轮(213)啮合带动第二从动齿轮(214)转动，从而带动混匀杯(212)旋转；
    所述驱动电机(211)固定在所述转盘(110)的圆周上；
    所述第二主动齿轮(213)外侧分布着第二从动齿轮(214)与之啮合；
    所述混匀杯(212)旋转时，带动安装在混匀杯(212)上的试剂管(220)内的生物聚合物混匀；和/或，所述混匀杯(212)旋转时，带动直接加入混匀杯(212)内的生物聚合物混匀。
  9. 如权利要求8所述的转盘旋转装置，其特征在于，所述混匀杯(212)和第二从动齿轮(214)固定在小轴(215)上；
    所述小轴(215)在转盘(110)的相应工位处安装有两个微型轴承(216、217)，所述小轴(215)穿过所述微型轴承(216、217)内孔间接地与转盘(110)连接，从而实现第二从动齿轮(214)相对转盘(110)圆周转动。
  10. 一种如权利要求1-9中任一项所述的带有混匀功能工位的转盘旋转装置的使用方法，其特征在于，包括以下步骤：
    步骤1：将装有生物样本的试剂管(220)插入混匀杯(212)或者将生物样本直接加入混匀杯内(212)；
    步骤2：启动驱动电机(211)，根据实际工艺需要单独控制每个驱动电机(211)的转速和方向，设置混匀杯(212)正反转；
    步骤3：将完成混匀的生物样本通过转盘(110)的转动到达设定的样本处理位，进行下一步操作；
    步骤4：通过转盘(110)的转动，连续处理多个生物样本。
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