WO2022143113A1 - Levure riche en chrome ayant une absorption élevée de chrome, son procédé de préparation et son application - Google Patents
Levure riche en chrome ayant une absorption élevée de chrome, son procédé de préparation et son application Download PDFInfo
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- WO2022143113A1 WO2022143113A1 PCT/CN2021/137249 CN2021137249W WO2022143113A1 WO 2022143113 A1 WO2022143113 A1 WO 2022143113A1 CN 2021137249 W CN2021137249 W CN 2021137249W WO 2022143113 A1 WO2022143113 A1 WO 2022143113A1
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- Prior art keywords
- chromium
- yeast
- saccharomyces cerevisiae
- enriched yeast
- source
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- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 title claims abstract description 284
- 239000011651 chromium Substances 0.000 title claims abstract description 284
- 229910052804 chromium Inorganic materials 0.000 title claims abstract description 284
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims abstract description 179
- 238000010521 absorption reaction Methods 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims abstract description 7
- JOPOVCBBYLSVDA-UHFFFAOYSA-N chromium(6+) Chemical compound [Cr+6] JOPOVCBBYLSVDA-UHFFFAOYSA-N 0.000 claims abstract description 19
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 173
- 238000000855 fermentation Methods 0.000 claims description 40
- 230000004151 fermentation Effects 0.000 claims description 40
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 20
- 238000000034 method Methods 0.000 claims description 17
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 14
- 235000013336 milk Nutrition 0.000 claims description 13
- 239000008267 milk Substances 0.000 claims description 13
- 210000004080 milk Anatomy 0.000 claims description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 11
- HPCCGRCEBFBZQP-UHFFFAOYSA-N chromium;pyridine-3-carboxylic acid Chemical compound [Cr].OC(=O)C1=CC=CN=C1 HPCCGRCEBFBZQP-UHFFFAOYSA-N 0.000 claims description 11
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 10
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 10
- 229910052799 carbon Inorganic materials 0.000 claims description 10
- 229910052757 nitrogen Inorganic materials 0.000 claims description 10
- 229910052698 phosphorus Inorganic materials 0.000 claims description 10
- 239000011574 phosphorus Substances 0.000 claims description 10
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 9
- 229910052760 oxygen Inorganic materials 0.000 claims description 9
- 239000001301 oxygen Substances 0.000 claims description 9
- 235000013379 molasses Nutrition 0.000 claims description 8
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 8
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 8
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical group [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 8
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 7
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 7
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 7
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 7
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 7
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 241001465754 Metazoa Species 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- WSZOLRPXRPIUPK-WCCKRBBISA-N (2s)-2-amino-4-methylsulfanylbutanoic acid;chromium Chemical compound [Cr].CSCC[C@H](N)C(O)=O WSZOLRPXRPIUPK-WCCKRBBISA-N 0.000 claims description 5
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical group [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 5
- 229960001763 zinc sulfate Drugs 0.000 claims description 5
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 5
- 229940041514 candida albicans extract Drugs 0.000 claims description 4
- 229940046374 chromium picolinate Drugs 0.000 claims description 4
- GJYSUGXFENSLOO-UHFFFAOYSA-N chromium;pyridine-2-carboxylic acid Chemical compound [Cr].OC(=O)C1=CC=CC=N1.OC(=O)C1=CC=CC=N1.OC(=O)C1=CC=CC=N1 GJYSUGXFENSLOO-UHFFFAOYSA-N 0.000 claims description 4
- 239000011573 trace mineral Substances 0.000 claims description 4
- 235000013619 trace mineral Nutrition 0.000 claims description 4
- 239000012138 yeast extract Substances 0.000 claims description 4
- 239000003674 animal food additive Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 2
- 108010080698 Peptones Proteins 0.000 claims description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 2
- 235000019319 peptone Nutrition 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims 1
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 239000002351 wastewater Substances 0.000 abstract description 2
- 239000000047 product Substances 0.000 description 54
- 230000000052 comparative effect Effects 0.000 description 24
- 238000012360 testing method Methods 0.000 description 14
- 235000013305 food Nutrition 0.000 description 7
- 239000002994 raw material Substances 0.000 description 7
- 235000011114 ammonium hydroxide Nutrition 0.000 description 5
- 229910021555 Chromium Chloride Inorganic materials 0.000 description 4
- QSWDMMVNRMROPK-UHFFFAOYSA-K chromium(3+) trichloride Chemical compound [Cl-].[Cl-].[Cl-].[Cr+3] QSWDMMVNRMROPK-UHFFFAOYSA-K 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- -1 and at the same time Chemical compound 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000009931 harmful effect Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 208000001647 Renal Insufficiency Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000036983 biotransformation Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000010410 dusting Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 201000006370 kidney failure Diseases 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 210000002747 omentum Anatomy 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical group [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/85—Saccharomyces
- C12R2001/865—Saccharomyces cerevisiae
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Definitions
- the invention relates to the technical field of microorganism application, in particular to a chromium-enriched yeast with high chromium absorption rate and a production method and application thereof.
- Chromium yeast is one of the sources of chromium nutritional supplements designated by the State Food and Drug Administration.
- Yeast is the earliest and most widely used pure natural nutritional microorganism, and it is also a natural nutritional treasure and an ideal biological carrier.
- Yeast chromium is to cultivate yeast cells in a medium containing trivalent chromium, and convert inorganic chromium into organic chromium through biotransformation, so as to improve the absorption and utilization rate of chromium in the body, reduce its toxic and side effects, and better play its role. Regulates blood sugar, lipid-lowering and cholesterol-lowering effects.
- the existing products mainly use Saccharomyces cerevisiae as a strain, add carbon, nitrogen and phosphorus sources, and use inorganic chromium as a chromium source. After fermentation and culture, the chromium-enriched yeast yeast milk is finally obtained, and then the product is obtained by drying.
- inorganic chromium has the following defects: the absorption rate is low, generally less than 10%; it has no biological activity and needs to be converted into biologically active GTF chromium to regulate metabolism. effect.
- the body of patients with diabetes and coronary heart disease basically does not have this transformation ability; the harmful effects of inorganic chromium have been found in animal experiments.
- the problem of the prior art solved by the present invention is as follows: the existing products mainly use Saccharomyces cerevisiae as a strain, add carbon, nitrogen and phosphorus sources, and use inorganic chromium as a chromium source. product.
- inorganic chromium there are more or less inorganic chromium in the existing products, the absorption rate of inorganic chromium is low, and it needs metabolic regulation to play a role, and some patients cannot even use it.
- inorganic chromium is easy to produce and trace amount of hexavalent chromium in the process product of chromium source, and trace amount of hexavalent chromium also exists in the chromium-enriched yeast in the present technology. Hexavalent chromium is harmful to human and animal health.
- the invention adopts organic chromium as the chromium source, eliminates the inorganic chromium source, the main raw material of the present invention (the chromium source is organic chromium) from the source, and uses the organic chromium raw material to produce the production process of chromium-enriched yeast, and there is no inorganic chromium in the finished product. , and the product has higher chromium content and chromium absorption rate.
- the present invention proposes the following technical solutions.
- the invention provides a chromium-enriched yeast, wherein the mass content of chromium in the chromium-enriched yeast is greater than 38,000 ppm, the organic chromium ratio in the chromium-enriched yeast is greater than 97% by mass, and the chromium-enriched yeast has a chromium absorption rate by mass. >50%, there is no hexavalent chromium in the chromium-rich yeast.
- the mass content of chromium in the chromium-enriched yeast is greater than 40,000 ppm
- the organic chromium ratio in the chromium-enriched yeast is greater than 95% by mass
- the chromium-enriched yeast is more than 60% by mass in the absorption rate of chromium.
- the present invention also provides the preparation method of the above-mentioned chromium-enriched yeast, characterized in that it comprises the following steps:
- Step 1 Amplify Saccharomyces cerevisiae to provide Saccharomyces cerevisiae seeds required for fermentation;
- Step 2 transfer the Saccharomyces cerevisiae seeds obtained in step 1 into a 50L fermentation tank to continue fermentation, and when the wet weight of Saccharomyces cerevisiae reaches 60-90g/L, add chromium source;
- Step 3 growing the Saccharomyces cerevisiae obtained in Step 2, when the wet weight of the yeast reaches 120-140g/L, continue fermentation to obtain yeast milk;
- Step 4 separate the yeast milk obtained in step 3, and then dry to obtain the chromium-enriched yeast;
- the chromium source is organic chromium
- the organic chromium is selected from one or more of chromium nicotinate, chromium picolinate and chromium methionine.
- the fermentation temperature is 25-35° C., preferably, the dissolved oxygen is less than or equal to 30 ⁇ mol/L, and more preferably, the concentration of ethanol is less than or equal to 0.3%.
- the chromium source is added in flow, preferably, the amount of the chromium source is 2-4mol, the chromium The source is all added within 2-10h.
- the pH is controlled to be 3.9-4.5. 18h.
- the pH is raised to 5-8 within 1-4 hours before the end of the fermentation, until the end of the fermentation, to obtain yeast milk.
- Saccharomyces cerevisiae described in step 1 is selected from Saccharomyces cerevisiae Z1.3 (Saccharomyces cerevisiae HANSEN Z1.3), Saccharomyces cerevisiae Z2.1 (Saccharomyces cerevisiae HANSEN Z2.1) and Saccharomyces cerevisiae FX-2 (Saccharomyces cerevisiae HANSEN Z2.1) One or more of FX-2).
- the components of the medium used in the culture in steps 1-3 contain one or more of carbon sources, nitrogen sources, phosphorus sources and trace elements.
- the carbon source is selected from molasses and/or glucose
- the nitrogen source is selected from one or more of ammonium sulfate, peptone, yeast extract and aqueous ammonia
- the phosphorus source is selected from dihydrogen phosphate Potassium
- the trace element is selected from zinc sulfate and/or magnesium sulfate.
- the concentration of the molasses is 20-40% by mass, and the concentration of the glucose is 20-40% by mass.
- the purity of the potassium dihydrogen phosphate is greater than 96%, the purity of the ammonium sulfate is greater than 99%, preferably, the purity of the zinc sulfate is greater than 99%, and the purity of the magnesium sulfate is greater than 99% %.
- the carbon source, the nitrogen source and the phosphorus source are added by means of feed addition.
- the present invention also provides a chromium-enriched yeast, which is characterized in that it is produced by the above-mentioned method.
- the present invention also provides the application of the chromium-enriched yeast in the field of animal husbandry, preferably the application in the field of feed or feed additive.
- the invention adopts organic chromium to replace traditional inorganic chromium as the chromium source, the absorption rate of chromium by yeast is greatly improved, and the absorption rate of the shake flask is increased from 1% to 2% to more than 60%;
- the total chromium of the chromium-enriched yeast can easily break through the upper limit of the traditional chromium-enriched yeast total chromium of 8000ppm, the minimum content is also above 38000ppm, can reach above 40000ppm, as high as 43096ppm, the mass content of organic chromium is more than 97%, and even can reach 98% The above; there is inorganic chromium that is difficult to wash in traditional chromium-enriched yeast.
- the new chromium-enriched yeast eliminates inorganic chromium from the source, and what remains is organic chromium, which is more conducive to human absorption and animal absorption; organic chromium sources are used to produce rich Chromium yeast can greatly reduce the chromium content in production wastewater and reduce environmental pressure; there is no hexavalent chromium in the chromium-enriched yeast produced by this process.
- the strain Saccharomyces cerevisiae Z1.3 (Saccharomyces cerevisiae Hansen Z1.3) used in the present invention has been biologically preserved in the China Center for Type Culture Collection (CCTCC, Wuhan University, zip code 430072) on October 25, 2005, The deposit number is CCTCC M 205125, and this strain has been recorded in the patent publication with the application number CN200610066365.X.
- Saccharomyces cerevisiae FX-2 Saccharomyces cerevisiae FX-2 used in the present invention was deposited in the China Center for Type Culture Collection (CCTCC) on August 1, 2016, and the deposit number is CCTCC NO: M2016418, and the deposit address is China. Wuhan. Wuhan University, Postal Code: 430072; Tel: (027)-68754052, the strain has been recorded in the patent publication with the application number CN201611141122.8.
- the strain Saccharomyces cerevisiae Z2.1 (Saccharomyces cerevisiae Hansen Z2.1) used in the present invention was preserved in the China Center for Type Culture Collection on October 25, 2005, the strain preservation number is CCTCC NO: M205127, and the preservation address is: China . Wuhan. Wuhan University, Postcode: 430072, Tel: (027) 68752319, the strain has been recorded in the patent publication with the application number CN201710522840.8.
- the invention provides a chromium-enriched yeast, wherein the mass content of chromium in the chromium-enriched yeast is greater than 38,000 ppm, the organic chromium ratio in the chromium-enriched yeast is greater than 97% by mass, and the chromium-enriched yeast has a chromium absorption rate by mass. >50%, there is no hexavalent chromium in the chromium-rich yeast.
- the mass content of chromium in the chromium-enriched yeast is greater than 40,000 ppm
- the organic chromium ratio in the chromium-enriched yeast is greater than 95% by mass
- the chromium-enriched yeast is more than 60% by mass in the absorption rate of chromium.
- the present invention also provides the preparation method of the above-mentioned chromium-enriched yeast, characterized in that it comprises the following steps:
- Step 1 Amplify Saccharomyces cerevisiae to provide Saccharomyces cerevisiae seeds required for fermentation;
- Step 2 transfer the Saccharomyces cerevisiae seeds obtained in step 1 into a 50L fermentation tank to continue fermentation, and when the wet weight of Saccharomyces cerevisiae reaches 60-90g/L, add chromium source;
- Step 3 growing the Saccharomyces cerevisiae obtained in step 2, when the wet weight of the yeast reaches 120-140 g/L, continue fermentation to obtain yeast milk;
- Step 4 separate the yeast milk obtained in step 3, and then dry to obtain the chromium-enriched yeast;
- the chromium source is organic chromium
- the organic chromium is selected from one or more of chromium nicotinate, chromium picolinate and chromium methionine.
- the present invention also provides a chromium-enriched yeast, which is characterized in that it is produced by the above-mentioned method.
- the present invention also provides the application of the chromium-enriched yeast in the field of animal husbandry, preferably the application in the field of feed or feed additive.
- the mass content of chromium in the chromium-enriched yeast is 0-40000 ppm, that is, the amount of chromium absorption is calculated by mass, and the chromium content per gram of yeast is 0-0.04 g;
- Chromium absorption rate total chromium mass content in yeast (product) / mass content of chromium in the added chromium source;
- organic chromium ratio intracellular organic chromium mass content/yeast (product) total chromium mass content
- the chromium content in the chromium-enriched yeast of the present invention refers to the chromium mass content of beneficial trivalent chromium, and at the same time, hexavalent chromium cannot exist.
- Chromium source chromium nicotinate or chromium methionine or chromium pyridinecarboxylate
- the yeast milk is dusted by a dusting and drying tower to obtain a chromium-enriched yeast product.
- Step 1 the Saccharomyces cerevisiae Chromium-enriched Saccharomyces cerevisiae Z1.3 (Saccharomyces cerevisiae HANSEN Z1.3) is picked a ring to carry out shake-flask culture—the step-by-step amplification culture of seed culture, to provide the Saccharomyces cerevisiae seeds required for fermentation;
- the components of the culture medium in the shake flask are: 100 g of sucrose, 20 g of yeast extract, 1 g of magnesium sulfate, 1 g of potassium dihydrogen phosphate, pH 4.8, cultured at 31°C for 24 hours, 5L shake flask with a liquid volume of 1L, and the shaker rotation speed 180rpm/ min;
- Step 2 transfer the Saccharomyces cerevisiae seeds of step 1 gained into 50L fermentor, control fermentation temperature to be 35 °C, dissolved oxygen 30 ⁇ mol/L, after 2 hours of fermentation, flow the carbon source (molasses concentration is 30%) of 4000g.
- Step 3 add 8000g carbon source (molasses concentration is 30%), 200g nitrogen source (ammonium sulfate), 200g phosphorus source (potassium dihydrogen phosphate), control the ethanol concentration to be 0.07% ⁇ 0.03%, for the growth of Saccharomyces cerevisiae , when the wet weight of the yeast reaches 120g/L, continue to maintain the pH at 3.9, control the dissolved oxygen at 30 ⁇ mol/L by ventilation and stirring, continue the fermentation for 9h, start 4h before the end of the fermentation, and gradually increase the pH to 6 with ammonia water , until the end of fermentation, to obtain yeast milk;
- Step 4 The chromium-enriched yeast product A is obtained by spray-drying the yeast milk obtained in step 3 after separating impurities.
- the chromium content of the prepared chromium-enriched yeast product A is 40563 ppm; the organic chromium ratio is 98.39%; the chromium absorption rate is 66.45%, and the prepared Chromium-enriched yeast product A has no hexavalent chromium.
- the steps are the same as those in Example 1, except that the chromium source fed in the step 2 is 3 mol of chromium methionine.
- the chromium content of the prepared chromium-enriched yeast product B was 38451 ppm; the organic chromium ratio was 97.78%; the chromium absorption rate was 61.03%, and the prepared chromium-enriched yeast product B had no hexavalent chromium.
- the steps are the same as in Example 1, except that the chromium source streamed in the step 2 is 3mol chromium picolinate.
- the prepared chromium-enriched yeast product C has a chromium content of 43096 ppm, an organic chromium ratio of 98.78%, and a chromium absorption rate of 68.4%.
- the prepared chromium-enriched yeast product C has no hexavalent chromium.
- Example 2 The rest of the experimental steps are the same as in Example 1, except that when the wet weight of Saccharomyces cerevisiae reaches 90 g/L in step 2, after adjusting the pH to 3.9, the chromium source (0.01 mol/L chromium nicotinate for a total of 3 mol , the chromium source is all added within 10h, and then the chromium-enriched yeast product D is obtained.
- the prepared chromium-enriched yeast product D has a chromium content of 41,025 ppm, an organic chromium ratio of 98.78%, and a chromium absorption rate of 65.18%. There is no hexavalent chromium in the chromium-enriched yeast product D.
- step 3 when the wet weight of the yeast reaches 140 g/L, the pH is controlled to 3.9, the dissolved oxygen is controlled to 30 ⁇ mol/L by ventilation and stirring, and the fermentation is continued for 18 h.
- the pH was raised to 6 within 4 hours before the end of the fermentation until the end of the fermentation to obtain yeast milk, and then the chromium-enriched yeast product E was obtained.
- the prepared chromium-enriched yeast product E has a chromium content of 40127 ppm, an organic chromium ratio of 98.67%, and a chromium absorption rate of 63.76%.
- the prepared chromium-enriched yeast product E has no hexavalent chromium.
- step 2 the fermentation temperature of the Saccharomyces cerevisiae seeds obtained in step 1 is controlled to be 25°C; afterward, the chromium-enriched yeast product F is obtained.
- the prepared chromium-enriched yeast product F has a chromium content of 41357 ppm, an organic chromium ratio of 98.88%, and a chromium absorption rate of 65.71%.
- the prepared chromium-enriched yeast product F has no hexavalent chromium.
- step 2 when the wet weight of Saccharomyces cerevisiae reaches 60 g/L, after adjusting the pH to 4.5, maintain the pH at 4.5 (the pH drops and then adds ammonia water to raise the pH to 4.5). ); and then the chromium-enriched yeast product G was obtained.
- the prepared chromium-enriched yeast product G has a chromium content of 40357 ppm, an organic chromium ratio of 98.38%, and a chromium absorption rate of 64.12%.
- the prepared chromium-enriched yeast product G has no hexavalent chromium.
- Example 2 The rest of the experimental conditions are the same as those in Example 1, except that the chromium source fed in in step 2 is chromium chloride; the chromium-enriched yeast product D1 is then obtained.
- the chromium content of the prepared chromium-enriched yeast product D1 was 2044.78ppm; the organic chromium ratio was 89.71%; the chromium absorption rate was 2.22%; and the hexavalent chromium was 0.933ppm.
- step 2 chromium nicotinate is not added, and in step 3, when the wet weight reaches 120 g/L, 3 mol of chromium nicotinate is added within 1 hour; then the chromium-enriched yeast product D2 is obtained. .
- the total chromium content in the chromium-enriched yeast product D2 was 32647ppm, the absorption rate of chromium was 51.87%, and the proportion of organic chromium was 98.52%.
- Example 2 The rest of the experimental conditions are the same as in Example 1, except that 3 mol of chromium nicotinate was added at the beginning of the fermentation in step 3, and the total amount of chromium nicotinate added was exactly the same as in Example 1; the chromium-enriched yeast product D3 was obtained later.
- the total chromium content in the chromium-enriched yeast product D3 was 35612ppm, the absorption rate of chromium was 56.58%, and the proportion of organic chromium was 98.41%.
- Example 2 The rest of the experimental conditions are the same as in Example 1, the difference is only that the fermentation pH is controlled at 5.5 in Steps 2 and 3, and then the chromium-enriched yeast product D4 is obtained.
- the total chromium content in the chromium-enriched yeast product D4 was 31985ppm, the absorption rate of chromium was 50.82%, and the proportion of organic chromium was 97.89%.
- Example 2 The rest of the experimental conditions are the same as in Example 1, the only difference is that the fermentation temperature is controlled at 38° C. in Steps 2 and 3; then the chromium-enriched yeast product D5 is obtained.
- the total chromium content in the chromium-enriched yeast product D5 was 26854ppm, the absorption rate of chromium was 42.67%, and the proportion of organic chromium was 98.14%.
- step 3 the alcohol of the fermentation broth is controlled at 1.0%; then the chromium-enriched yeast product D6 is obtained.
- the total chromium content in the chromium-enriched yeast product D6 is 19688ppm, the absorption rate of chromium is 31.28%, and the proportion of organic chromium is 98.36%.
- step 3 the dissolved oxygen in the fermentation broth is controlled at 60 ⁇ mol/L; then the chromium-enriched yeast product D7 is obtained.
- the total chromium content in the chromium-enriched yeast product D7 was 22457ppm, the absorption rate of chromium was 35.68%, and the proportion of organic chromium was 98.41%.
- Example 1 40563 98.39 66.45
- Example 2 38451 97.78 61.03
- Example 3 43096 98.78 68.4 Example 4 41025 98.78 65.18
- Example 5 40127 98.67 63.76
- Example 6 41357 98.88 65.71
- Example 7 40357 98.38 64.12 Comparative Example 1 2044.78 89.71 2.22
- Comparative Example 2 32647 98.52 51.87
- Comparative Example 3 35612 98.41 56.58 Comparative Example 4 31985 97.89 50.82
- Comparative Example 6 19688 98.36 31.28 Comparative Example 7 22457 98.41 35.68
- Comparative Example 4 shows that fermentation pH has a significant impact on the absorption rate of chromium; Comparative Example 5 is compared with Example 1, and it can be seen that fermentation temperature has a significant impact on the absorption rate of chromium; Comparative Example 6 Compared with Example 1, it can be seen that the alcohol content of the fermentation broth has a significant impact on the absorption rate of chromium; in Comparative Example 7, compared with Example 1, it can be seen that the dissolved oxygen in the fermentation broth has a significant impact on the absorption rate of chromium.
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Abstract
L'invention concerne une levure riche en chrome, son procédé de préparation et son application. La levure riche en chrome a une teneur massique en chrome supérieure à 38 000 ppm, une proportion de chrome organique supérieure à 97 % en masse et un taux d'absorption du chrome supérieur à 50 % en masse, et elle ne contient pas de chrome hexavalent. Le procédé de préparation de la levure riche en chrome utilise du chrome organique pour remplacer le chrome inorganique classique en tant que source de chrome. L'absorption du chrome par la levure est considérablement augmentée, l'absorption du chrome est élevée, et la teneur en chrome dans les eaux usées produites est fortement réduite, ce qui réduit les contraintes liées à la protection de l'environnement.
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CN1223863A (zh) * | 1998-12-09 | 1999-07-28 | 中国科学院高能物理研究所 | 醋酸铬酵母的制备 |
US6248323B1 (en) * | 1996-09-25 | 2001-06-19 | Viva Life Science, Inc. | Dietary supplementation with and methods for preparation of yeast-derived chromium salts |
CN1366037A (zh) * | 2001-01-17 | 2002-08-28 | 中国科学院微生物研究所 | 含有高生物量的富铬酵母及其生产方法 |
CN1482236A (zh) * | 2003-07-25 | 2004-03-17 | 贵州天安药业股份有限公司 | 一种用食用酵母制备富铬酵母的方法 |
CN101045906A (zh) * | 2006-03-30 | 2007-10-03 | 安琪酵母股份有限公司 | 一种富铬酿酒酵母、富铬酵母产品及其生产方法 |
CN101372674A (zh) * | 2007-08-22 | 2009-02-25 | 安琪酵母股份有限公司 | 富铬酵母的生产方法 |
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CN101575617B (zh) * | 2009-03-06 | 2013-09-11 | 广州市博善生物饲料有限公司 | 一种富铬酵母培养物及其发酵工艺 |
CN103396955B (zh) * | 2013-07-15 | 2015-03-04 | 浙江深友生物技术有限公司 | 一种酵母及利用这种酵母高密度发酵生产富铬酵母的方法 |
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US6248323B1 (en) * | 1996-09-25 | 2001-06-19 | Viva Life Science, Inc. | Dietary supplementation with and methods for preparation of yeast-derived chromium salts |
CN1223863A (zh) * | 1998-12-09 | 1999-07-28 | 中国科学院高能物理研究所 | 醋酸铬酵母的制备 |
CN1366037A (zh) * | 2001-01-17 | 2002-08-28 | 中国科学院微生物研究所 | 含有高生物量的富铬酵母及其生产方法 |
CN1482236A (zh) * | 2003-07-25 | 2004-03-17 | 贵州天安药业股份有限公司 | 一种用食用酵母制备富铬酵母的方法 |
CN101045906A (zh) * | 2006-03-30 | 2007-10-03 | 安琪酵母股份有限公司 | 一种富铬酿酒酵母、富铬酵母产品及其生产方法 |
CN101372674A (zh) * | 2007-08-22 | 2009-02-25 | 安琪酵母股份有限公司 | 富铬酵母的生产方法 |
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