WO2022126948A1 - Traditional chinese medicine composition and use thereof in preparation of drug for treating indirect hernia - Google Patents

Abstract

A traditional Chinese medicine composition for treating an indirect hernia, and a preparation method therefor and the use thereof. The raw materials of the composition are spiders, Cinnamomi ramulus, Bupleuri radix, Astragali radix, Cimicifugae rhizoma, Zingiberis rhizoma recens and Borneolum syntheticum.

Description

Traditional Chinese medicine composition and its application in the preparation of medicine for treating fox temples

This application claims the priority of the Chinese patent application filed on December 14, 2020, with the application number 202011472303.5 and the invention titled "Chinese medicine composition and its application in the preparation of medicine for the treatment of fox temples", all of which are The contents are incorporated herein by reference.

technical field

The invention relates to the technical field of traditional Chinese medicine, in particular to a traditional Chinese medicine composition and its application in preparing a medicine for treating fox hernia.

Background technique

Fox hernia, the name of the disease in traditional Chinese medicine. It is caused by the loss of liver qi, or when children and elderly people have weak qi, or when the intra-abdominal pressure increases, the intestines and other abdominal organs slide into the scrotum. A hernia disease characterized by a lump in the groin, sometimes big or small, full of pain, such as fox's impermanence. "Golden Chamber Synopsis" records: "The hernia of the yin fox is small and large, and it is always up and down, and the spider is scattered." This clearly points out the clinical characteristics and the main treatment of the hernia of the yin fox. Fox hernia is equivalent to "hernia" and "indirect inguinal hernia" in modern medicine. Traditional Chinese medicine believes that it is a disease caused by cold and dampness and other evil qi condensing in the liver meridian of foot Jueyin, or the disorder of qi machine, which is caused by the scrotum.

If the fox hernia is diagnosed early, the condition is mild, and the prognosis is generally good. If the disease is severe and the righteousness is weak, some patients may also be accompanied by complications such as intestinal obstruction, abdominal pain, and shock. The spider powder composed of spiders and cinnamon sticks recorded in the Synopsis of the Golden Chamber is the most famous traditional Chinese medicine prescription. But in modern times, there are few researches on traditional Chinese medicine prescriptions for the treatment of fox hernia.

SUMMARY OF THE INVENTION

In view of this, the technical problem to be solved by the present invention is to provide a traditional Chinese medicine composition and its application in the preparation of a medicament for treating fox hernia, in order to more effectively treat fox hernia.

The traditional Chinese medicine composition provided by the present invention, its raw materials include spider, cinnamon stick, Bupleurum, astragalus, cohosh, ginger and borneol.

In some embodiments, the traditional Chinese medicine composition is made from the following raw materials by mass: 4-6 parts of spider, 80-120 parts of cinnamon stick, 80-120 parts of Bupleurum, 50-70 parts of Astragalus, 25-35 parts of Cimicifuga servings, 80-120 servings of ginger, and 4-6 servings of borneol.

In some specific embodiments, it is made from the following raw materials by mass: 5 parts of spiders, 100 parts of cinnamon sticks, 100 parts of Bupleurum, 60 parts of astragalus, 30 parts of cohosh, 100 parts of ginger, and 5 parts of borneol.

The preparation method of described Chinese medicine composition, comprises:

The cassia twig and ginger are extracted by steam distillation to obtain volatile oil;

Extracting spider, Bupleurum, Astragalus and Cimicifuga with aqueous ethanol solution to obtain an extract;

The borneol is dissolved in an aqueous ethanol solution to obtain a borneol solution;

The traditional Chinese medicine composition is prepared by adjusting the volume fraction of ethanol in the mixture to 28-32% with water and ethanol with a volume fraction of 85% to 95%.

In the present invention, the preparation method of the volatile oil comprises the following steps: after soaking the cassia twig and ginger in water, steam distillation for 1 hour; and extracting the combined extracts twice to obtain the volatile oil.

The infiltration refers to the degree of moisturizing the cinnamon sticks and ascending and descending, but no water flow.

In the step of preparing the extract in the present invention, the volume fraction of ethanol in the ethanol aqueous solution is 60%.

In the step of preparing the extract of the present invention, the mass-volume ratio of the sum of the mass of spider, Bupleurum, Astragalus, and Cimicifuga to the ethanol aqueous solution is 100 g: 600 mL.

In the step of preparing the extraction solution of the present invention, the extraction is reflux extraction, and the time for each extraction is 1.5 hours. Extract in two steps, combine the extracts, and concentrate to a relative density of 1.15.

The volume fraction of ethanol in the ethanol aqueous solution for preparing the borneol solution in the present invention is 90%.

In the present invention, the volatile oil, the extract, and the borneol solution are mixed, and the volume ratio of the mixing is 10:420:20.

After the mixing, the volume fraction of ethanol is adjusted to 30% with water and a 90% ethanol solution to prepare a traditional Chinese medicine composition.

Application of the traditional Chinese medicine composition of the present invention, or the traditional Chinese medicinal composition prepared by the preparation method, in the preparation of a medicine for treating fox hernia.

The present invention also provides a medicine for treating fox hernia, which comprises the composition of the present invention.

The medicine for treating fox hernia of the present invention is in the form of a spray.

The present invention also provides a method for treating fox hernia, which is to administer the medicine of the present invention.

The raw materials of the composition provided by the present invention include spider, cinnamon stick, Bupleurum, astragalus, cohosh, ginger and borneol. It uses spider as the monarch medicine, with Chinese medicinal materials such as Guizhi and Bupleurum as the minister medicine and auxiliary medicine, which plays a good role in the treatment of fox hernia, and its effect is significantly better than the traditional Jinfang spider powder.

Detailed ways

The invention provides a traditional Chinese medicine composition and its application in the preparation of a medicine for treating fox hernia, and those skilled in the art can learn from the content of this article and appropriately improve the process parameters to achieve. It should be particularly pointed out that all similar substitutions and modifications are obvious to those skilled in the art, and they are deemed to be included in the present invention. The method and application of the present invention have been described through the preferred embodiments, and it is obvious that relevant persons can make changes or appropriate changes and combinations of the methods and applications herein without departing from the content, spirit and scope of the present invention, so as to realize and apply the present invention. Invention technology.

The test materials used in the present invention are all common commercial products and can be purchased in the market.

The spider is the whole worm of Jingzhao tassular spider artificially cultivated by the applicant in the Wuzhishan spider breeding base. Bitter, cold, poisonous. Enters the Liver Meridian of Jueyin of Foot. Dispel wind, reduce swelling, and detoxify. Treatment of partial fall of fox hernia, crooked mouth after stroke, slow convulsion in children, choked mouth, malnutrition accumulation;

Bupleurum is the dried root of Bupleurum chinense DC. or Bupleurum scorzonerifolium Willd. The taste is bitter, slightly cold. Return to the liver and gallbladder meridians. Reconcile the inside and outside, soothe the liver and raise the yang. For colds and fever, cold and heat exchanges, chest and flank pain, irregular menstruation, prolapse of the genitals, prolapse of the anus.

Cinnamomum cassia twig is the dry twig of the Lauraceae plant Cinnamomum cassia Presl; nature and flavor: spicy, sweet, warm. Return to the heart, lung and bladder meridians. Sweating and releasing muscles, warming and dredging meridians, helping yang to transform qi, and calming chong and lowering qi. For wind-cold cold, abdominal cold pain, blood-cold amenorrhea, joint arthralgia, phlegm-drinking, edema, palpitations, beating pig.

Astragalus is the dried root of the legume Astragalus membranaceus (Fisch.) Bge.var. mongolicus (Bge.) Hsiao or Astragalus membranaceus (Fisch.) Bge. Sweet, warm. Return to the lung and spleen meridians. Invigorating Qi and solidifying the surface, diuretic support toxins, expelling pus, astringing sores and producing muscles. For qi deficiency and fatigue, lack of appetite and loose stools, depression of the middle qi, prolonged diarrhea and prolapse of the rectum, metrorrhagia and bleeding in the stool, spontaneous perspiration on the surface, edema due to qi deficiency, difficult ulceration of carbuncle, prolonged ulceration without restraint, blood deficiency and yellowing, internal heat and quenching thirst; chronic nephritis proteinuria, diabetes.

Cimicifuga is the dried rhizome of the Ranunculaceae plant Cimicifuga heracleifolia Kom., Cimicifuga dahurica (Turcz.) Maxim. or Cimicifuga foetida L.. Pungent in nature, slightly sweet, slightly cold. Returns the lung, spleen, stomach, and large intestine meridians. It expresses rash, clears heat and detoxifies, and lifts yang. For wind-heat headache, toothache, mouth sore, sore throat, measles opacity, yang poisoning spots; prolapse of the anus, uterine prolapse.

Ginger is the fresh rhizome of the ginger plant Zingiber officinale Rosc. Returns the lung, spleen and stomach meridians. Relieves the exterior and dispels cold, warms the middle to stop vomiting, resolves phlegm and stops cough. For wind-cold cold, stomach-cold vomiting, cough with cold phlegm.

Borneol, also known as synthetic borneol, plum slices, moxa powder, and knot slices, is acrid, bitter and slightly cold in nature. Return to the heart, spleen and lung meridians. Refreshing, clearing heat and relieving pain. For fever dizziness, convulsions, stroke phlegm Jue, Qi stagnation violent Jue, middle aversion coma, red eyes, mouth sores, sore throat, ear canal pus.

Below in conjunction with embodiment, the present invention is further elaborated:

Example 1

Prescription: spider dried powder 5g, cinnamon stick 100g, Bupleurum 100g, astragalus 60g, cohosh 30g, ginger 100g, borneol 5g.

Production method:

1. Put cinnamon sticks and ginger in a multifunctional extractor, add an appropriate amount of water, and fully infiltrate. To be moist but see no water flow. Steam distillation was directly passed through the extractor for 1 hour. Continue to add an appropriate amount of water and steam distillation for 1 hour. About 10 mL of volatile oil from the secondary extraction was collected and used for later use.

2. Other raw materials (except borneol) were refluxed at room temperature with 1170 mL of 60% ethanol by volume, and extracted twice for 1.5 hours each time.

3. The borneol was dissolved in 20 mL of 90% ethanol to obtain a borneol solution.

4. Combine the solutions of items 1, 2 and 3 (volatile oil: extract: borneol solution = 10:420:20), use pure water and 90% ethanol by volume to obtain 1000 mL of ethanol with a volume fraction of 30±2%. preparation.

5. Fill it as a spray, spray the affected area (small stomach to groin, genitals), 2 times a day.

Comparative Example 1

Prescription: Guizhi 100g, Bupleurum 100g, Astragalus 60g, Cimicifuga 30g, Ginger 100g, Borneol 5g.

Production method:

1. Put cinnamon sticks and ginger in a multifunctional extractor, add an appropriate amount of water, and fully infiltrate. To be moist but see no water flow. Steam distillation was directly passed through the extractor for 1 hour. Continue to add an appropriate amount of water and steam distillation for 1 hour. About 10 mL of volatile oil from the secondary extraction was collected and used for later use.

2. Other raw materials (except borneol) were refluxed with 1140 mL of 60% ethanol at room temperature, and extracted twice for 1.5 hours each time. The extract was collected, filtered and concentrated to a relative density of 1.15 to obtain about 430 mL of extract.

3. The borneol was dissolved in 20 mL of 90% ethanol to obtain a borneol solution.

4. Combine the solutions of items 1, 2 and 3 (volatile oil: extract: borneol solution = 10:430:20), use pure water and 90% ethanol by volume to obtain 1000 mL of ethanol with a volume fraction of 30±2%. preparation.

5. Fill it as a spray, spray the affected area (small stomach to groin, genitals), 2 times a day.

Comparative Example 2

Prescription: Spider dried powder 5g, Bupleurum 100g, Astragalus 60g, Cimicifuga 30g, Ginger 100g, Borneol 5g.

Production method:

1. Put the ginger in a multifunctional extractor, add an appropriate amount of water, and fully infiltrate it. To be moist but see no water flow. Steam distillation was directly passed through the extractor for 1 hour. Continue to add an appropriate amount of water and steam distillation for 1 hour. About 7 mL of volatile oil from the secondary extraction was collected and used for later use.

2. Other raw materials (except borneol) were refluxed with 1170 mL of 60% ethanol at room temperature, and extracted twice, 1.5 hours each time, the extract was collected, filtered, combined and concentrated to a relative density of 1.15 to obtain about 412 mL of extract.

3. The borneol was dissolved in 20 mL of 90% ethanol to obtain a borneol solution.

4. Combine the solutions of items 1, 2 and 3 (volatile oil: extract: borneol solution = 7:412:20), use pure water and 90% ethanol by volume to obtain 1000 mL of ethanol with a volume fraction of 30±2%. preparation.

5. Fill it as a spray, spray the affected area (small stomach to groin, genitals), 2 times a day.

Comparative Example 3

Prescription: spider dried powder 5g, cinnamon stick 100g, astragalus 60g, cohosh 30g, ginger 100g, borneol 5g.

Production method:

1. Put cinnamon sticks and ginger in a multifunctional extractor, add an appropriate amount of water, and fully infiltrate. To be moist but see no water flow. Steam distillation was directly passed through the extractor for 1 hour. Continue to add an appropriate amount of water and steam distillation for 1 hour. About 10 mL of volatile oil from the secondary extraction was collected and used for later use.

2. Other raw materials (except borneol) were refluxed with 570 mL of 60% ethanol at room temperature, and extracted twice for 1.5 hours each time. The extract was collected, filtered, combined and concentrated to a relative density of 1.15 to obtain about 210 mL of extract.

3. The borneol was dissolved in 20 mL of 90% ethanol to obtain a borneol solution.

4. Combine the solutions of items 1, 2 and 3 (volatile oil: extract: borneol solution = 10:210:20), use pure water and 90% ethanol by volume to obtain 1000mL of ethanol with a volume fraction of 30±2%. preparation.

5. Fill it as a spray, spray the affected area (small stomach to groin, genitals), 2 times a day.

Comparative Example 4

Prescription: spider dried powder 5g, cinnamon stick 100g, Bupleurum 100g, cohosh 30g, ginger 100g, borneol 5g.

Production method:

1. Put cinnamon sticks and ginger in a multifunctional extractor, add an appropriate amount of water, and fully infiltrate. To be moist but see no water flow. Steam distillation was directly passed through the extractor for 1 hour. Continue to add an appropriate amount of water and steam distillation for 1 hour. About 10 mL of volatile oil from the secondary extraction was collected and used for later use.

2. Other raw materials (except borneol) were refluxed with 810 mL of 60% ethanol at room temperature, and extracted twice for 1.5 hours each time. The extract was collected, filtered, combined and concentrated to a relative density of 1.15 to obtain about 330 mL of extract.

3. The borneol was dissolved in 20 mL of 90% ethanol to obtain a borneol solution.

4. Combine the solutions of items 1, 2 and 3 (volatile oil: extract: borneol solution = 10:330:20), use pure water and 90% ethanol by volume to obtain 1000 mL of ethanol with a volume fraction of 30±2%. preparation.

5. Fill it as a spray, spray the affected area (small stomach to groin, genitals), 2 times a day.

Comparative Example 5

Spider Powder (Spider + Guizhi), from "The Brief Introduction of the Golden Chamber".

Efficacy evaluation:

The formulations of Example 1 and Comparative Examples 1 to 5 were prepared in a hospital of traditional Chinese medicine in Hainan Province, and clinical trials were carried out in the professional clinic of Li nationality medicine. After more than 3 years of clinical verification, this technical method has a significant effect on the treatment of fox hernia.

140 clinical trial subjects were selected and divided into 7 groups, of which one group was used as a control group without treatment, and the other six groups were given the medicines made by the formulations of Examples and Comparative Examples 1 to 5 respectively. Spray twice for 20 consecutive days. The efficacy evaluation criteria are:

Clinical cure: all clinical symptoms disappeared; the main physical and chemical test indicators were normal.

Markedly effective: the clinical symptoms are eliminated or the main symptoms are eliminated; the physical and chemical test indicators are basically normal.

Effective: the main symptoms are basically eliminated; the main physical and chemical test indicators have improved.

Invalid: Compared with before treatment, the symptoms and main physical and chemical test indicators did not change or even worsened.

The specific test results are as follows.

Table 1 Therapeutic effect

group	cure	effective	efficient	invalid	Efficient/%
Example 1	13	3	2	2	90
Control 1 (missing spiders)	4	2	5	9	55
Control group 2 (lack of cinnamon sticks)	6	5	1	8	60
Control 3 (lack of Bupleurum)	8	3	4	5	75
Control 4 (lack of Astragalus)	9	2	5	4	80
Control group 5 (spider powder)	6	7	2	5	75
Control group 6 (untreated)	0	0	0	20	0

The results show that the preparation of the present invention has a significant curative effect on the treatment of fox hernia, and through the scientific compatibility of medicinal materials, its curative effect is stronger than that of the traditional Jinfang spider powder for treating fox hernia, and is also better than other control groups lacking a certain drug.

The above are only the preferred embodiments of the present invention. It should be pointed out that for those skilled in the art, without departing from the principles of the present invention, several improvements and modifications can also be made, and these improvements and modifications should also be regarded as It is the protection scope of the present invention.

Claims (10)

1. The traditional Chinese medicine composition, its raw materials include spider, cinnamon stick, Bupleurum, astragalus, cohosh, ginger and borneol.
2. The traditional Chinese medicine composition according to claim 1, characterized in that, it is made from the following raw materials by mass: 4-6 parts of spider, 80-120 parts of cinnamon, 80-120 parts of Bupleurum, 50-70 parts of astragalus , 25-35 servings of cohosh, 80-120 servings of ginger, and 4-6 servings of borneol.
3. traditional Chinese medicine composition according to claim 1, is characterized in that, it is made from the raw material of following mass parts: 5 parts of spider, 100 parts of cinnamon stick, 100 parts of Bupleurum, 60 parts of Astragalus, 30 parts of Cimicifuga, 100 parts of ginger portion, 5 portions of borneol.
4. The preparation method of the traditional Chinese medicine composition according to any one of claims 1 to 3, characterized in that, extracting cassia twig and ginger by steam distillation to obtain volatile oil;

   Extracting spider, Bupleurum, Astragalus and Cimicifuga with aqueous ethanol solution to obtain an extract;

   The borneol is dissolved in an aqueous ethanol solution to obtain a borneol solution;

   The volatile oil, the extract, and the borneol solution are mixed, and the volume fraction of ethanol in the mixture is adjusted to 28% to 32% with water and ethanol with a volume fraction of 85% to 95% to prepare a traditional Chinese medicine composition.
5. The preparation method according to claim 4, wherein the preparation method of the volatile oil comprises: after soaking the cassia twig and ginger with water, steam distillation for 1 hour; extracting the combined extracts twice to obtain the volatile oil.
6. The preparation method according to claim 4, wherein in the step of preparing the extract, the volume fraction of ethanol in the ethanol aqueous solution is 60%; The mass-volume ratio of the ethanol aqueous solution is 100 g: 600 mL; reflux at room temperature, the extraction times are 2 times, and the extracts are combined and concentrated to a solution density of 1.15 g/mL.
7. The preparation method according to claim 4, wherein the volume fraction of ethanol in the aqueous ethanol solution for preparing the borneol solution is 90%.
8. The preparation method according to claim 4, wherein the volatile oil, the extract, and the borneol solution are mixed, and the volume ratio of the mixing is 10:420:20.
9. Application of the traditional Chinese medicine composition according to any one of claims 1 to 4, or the traditional Chinese medicine composition prepared by the preparation method according to any one of claims 5 to 9, in the preparation of a medicine for treating fox hernia.
10. A medicine for treating fox hernia, comprising the traditional Chinese medicine composition according to any one of claims 1 to 4, or the traditional Chinese medicine composition prepared by the preparation method according to any one of claims 5 to 9.