WO2022111561A1 - Composition de malus neidzwetzkyana dieck et de prunus cerasifera ehrhart, et son utilisation dans le traitement antithrombotique - Google Patents

Composition de malus neidzwetzkyana dieck et de prunus cerasifera ehrhart, et son utilisation dans le traitement antithrombotique Download PDF

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WO2022111561A1
WO2022111561A1 PCT/CN2021/133009 CN2021133009W WO2022111561A1 WO 2022111561 A1 WO2022111561 A1 WO 2022111561A1 CN 2021133009 W CN2021133009 W CN 2021133009W WO 2022111561 A1 WO2022111561 A1 WO 2022111561A1
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red
cherry plum
apple
fruit
fleshed
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PCT/CN2021/133009
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English (en)
Chinese (zh)
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沈妍
张正方
韩铨
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浙江养生堂天然药物研究所有限公司
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Publication of WO2022111561A1 publication Critical patent/WO2022111561A1/fr

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
    • A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
    • A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
    • A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
    • A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
    • A23G3/364—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
    • A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
    • A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
    • A23G3/48—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds containing plants or parts thereof, e.g. fruits, seeds, extracts
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/01—Instant products; Powders; Flakes; Granules
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/03—Products from fruits or vegetables; Preparation or treatment thereof consisting of whole pieces or fragments without mashing the original pieces
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
    • A23L21/10—Marmalades; Jams; Jellies; Other similar fruit or vegetable compositions; Simulated fruit products
    • A23L21/12—Marmalades; Jams; Jellies; Other similar fruit or vegetable compositions; Simulated fruit products derived from fruit or vegetable solids
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/60—Salicylic acid; Derivatives thereof
    • A61K31/612—Salicylic acid; Derivatives thereof having the hydroxy group in position 2 esterified, e.g. salicylsulfuric acid
    • A61K31/616—Salicylic acid; Derivatives thereof having the hydroxy group in position 2 esterified, e.g. salicylsulfuric acid by carboxylic acids, e.g. acetylsalicylic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/736—Prunus, e.g. plum, cherry, peach, apricot or almond
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Definitions

  • the invention relates to the fields of food and medicine, in particular to a red meat apple and cherry plum composition and its application in antithrombotic.
  • a thrombus is a small block formed by blood flow on the surface of the inner surface of the cardiovascular system at the peeling or repairing site.
  • a thrombus consists of insoluble fibrin, deposited platelets, accumulated white blood cells, and trapped red blood cells.
  • Thrombotic diseases refer to diseases caused by the formation of thrombus in blood vessels and thromboembolism affecting blood flow, including myocardial infarction, ischemic cerebral infarction and venous thromboembolism.
  • thrombosis is the main cause of cardiovascular disease.
  • the drugs used clinically for the prevention and treatment of thrombotic diseases mainly include anticoagulant drugs, thrombolytic drugs and antiplatelet aggregation drugs, including aspirin, clopidogrel, heparin, warfarin, etc. But there is often a risk of bleeding with these drugs.
  • Aspirin mainly prevents the aggregation of platelets and has a stimulating effect on the gastrointestinal tract. Long-term use will damage the gastric mucosa, causing gastric ulcers and even gastric bleeding.
  • Clopidogrel is less likely than aspirin, but there is also a risk of bleeding. Warfarin and heparin have antithrombin effects, and common adverse reactions also carry the risk of bleeding. Therefore, it is necessary to find natural resources with good effects and no side effects for the prevention and treatment of thrombotic diseases.
  • Red-fleshed apple (Malus neidzwetzkyana Dieck) is a plant of the Rosaceae (Rosaceae) genus Malus Mill. Its fruit flesh is red, and the color of the fruit is mainly caused by the accumulation of anthocyanins.
  • red-fleshed apples there are many varieties of red-fleshed apples around the world, such as Hongxun series from Xinjiang, China, Robert Crab from the United States, Ruby Sweet from Japan, and Redlove from Switzerland. Different varieties of red-fleshed apples have different flowers, branch and leaf colors, fruit size, degree of coloration, taste and flavor. Red-fleshed apples in Xinjiang play an important role in the origin of red-fleshed apples.
  • Cherry plum (Prunus cerasifera Ehrhart), commonly known as wild sour plum, is an extremely precious and rare and endangered primitive wild forest fruit in the world. According to the records of "China Trees" and the research of the National Institute of Wild Plants, wild cherry plums are only found in the western Tianshan Mountains of Xinjiang and the Caucasus and Asia Minor mountains, and wild resources are extremely scarce. Cherry plum fruit has a long history of consumption in Xinjiang region of my country. The Kazakh and Mongolian herdsmen living in Daxigou, Tianshan mainly eat beef and mutton, but very few people suffer from high blood lipids and high blood pressure.
  • Red-fleshed apples and cherry plums are rich in a variety of amino acids, vitamins, minerals, and functional components such as anthocyanins, polyphenols, and flavonoids, and have high development value.
  • the current research is still relatively limited, mainly focusing on chemical components and antioxidants, and it is still in the preliminary research stage.
  • red-fleshed apple or red-flesh apple extract
  • cherry plum or cherry plum extract
  • the present invention provides the use of the composition in the preparation of medicines (such as traditional Chinese medicines, Chinese patent medicines, etc.), ordinary food or health food, the medicines (such as traditional Chinese medicines, Chinese patent medicines, etc.), Ordinary food or health food is used for antithrombotic or for preventing and/or treating thrombotic diseases, wherein the composition comprises:
  • a first component that is a red-fleshed apple or red-fleshed apple extract
  • the second component, the second component is cherry plum or cherry plum extract.
  • the thrombotic disease is selected from myocardial infarction, ischemic cerebral infarction, and venous thromboembolism and other related diseases caused by blood clots.
  • the weight ratio of the first component and the second component is 1:10-10:1 (such as 1:5-10:1, 1:2-10:1, 1:1) 1-10:1, 1:10-5:1, 1:5-5:1, 1:2-5:1, 1:1-5:1, 1:10-2:1, 1:5- 2:1, 1:2-2:1, 1:1-2:1, 1:10-1:1, 1:5-1:1, 1:2-1:1, 1:10-5: 1, 1:10-2:1, 1:10-1:1, 1:5-10:1, 1:5-5:1, 1:5-2:1, 1:5-1:1, 1:2-10:1, 1:2-5:1, 1:2-2:1, 1:2-1:1, 1:1-10:1, 1:1-5:1, 1: 1-2:1, such as 1:5, 1:2, 1:1, 2:1, 5:1).
  • the red-fleshed apple extract is obtained by one of the following extraction methods: leaching extraction, enzymatic hydrolysis ultrasonic extraction, ultra-high pressure extraction, subcritical extraction or other extraction methods.
  • the cherry plum extract is obtained by one of the following extraction methods: leaching extraction, enzymatic hydrolysis ultrasonic extraction, ultra-high pressure extraction, subcritical extraction or other extraction methods.
  • the composition is in the form of fresh fruit, fruit juice, jam, fruit powder (such as lyophilized powder), candy (such as tablet candy), oral liquid, capsule, tablet, dried fruit, preserved fruit, fruit wine or cake exist in other forms.
  • the product form of the composition can be fresh fruit, fruit juice, jam, fruit powder (such as lyophilized powder), candy (such as tablet candy), oral liquid, capsule, tablet, dried fruit, preserved fruit, fruit wine or pastries etc. That is to say, the composition can be made into fresh fruit, fruit juice, jam, fruit powder (such as freeze-dried powder), candy (such as tablet candy), oral liquid, capsule, tablet, dried fruit, preserved fruit, fruit wine or cake, etc. .
  • the red-fleshed apple refers to the fruit of the Rosaceae apple plant with red pulp.
  • the red meat apple is selected from the group consisting of: Hong Xun No. 1 (Hong Xun No. 1), Hong Xun No. 3 (Hong Xun No. 3), Hong Xun No. 6 (Hong Xun No. 6), Xia Hong Meat , Daihong, Red Love, Roberts Crab, Red Dream, Ruby Sweet, etc.
  • the cherry plum is selected from the group consisting of cherry plum red fruit, cherry plum black fruit, cherry plum purple fruit, cherry plum purple black fruit, cherry plum yellow fruit, and the like.
  • the present invention provides a combination product comprising:
  • a first product, the first product is the aforementioned composition
  • the second product, the second product is a drug for preventing and/or treating thrombotic diseases.
  • the thrombotic disease is selected from myocardial infarction, ischemic cerebral infarction, and venous thromboembolism and other related diseases caused by blood clots.
  • the drug for preventing and/or treating thrombotic diseases is selected from aspirin, clopidogrel, heparin, warfarin and the like.
  • the weight ratio of the first product and the second product is from 1000:2 to 1000:0.25 (eg, 1000:2, 1000:1.5, 1000:1, 1000:0.9, 1000:0.8, 1000:0.7, 1000:0.6, 1000:0.5, 1000:0.25; or 1000:1-1000:0.5, specifically 1000:1, 1000:0.9, 1000:0.8, 1000: 0.7, 1000:0.6, 1000:0.5).
  • the present invention provides the use of the aforementioned combination product in the preparation of medicine (such as traditional Chinese medicine, Chinese patent medicine, etc.), ordinary food or health food, the medicine (such as traditional Chinese medicine, Chinese patent medicine, etc.), ordinary Foods or health foods are used for antithrombotics, or for preventing and/or treating thrombotic diseases.
  • medicine such as traditional Chinese medicine, Chinese patent medicine, etc.
  • ordinary food or health food such as traditional Chinese medicine, Chinese patent medicine, etc.
  • ordinary Foods or health foods are used for antithrombotics, or for preventing and/or treating thrombotic diseases.
  • the present invention provides the aforementioned composition or the aforementioned combined product, which is used for antithrombotic, or for preventing and/or treating thrombotic diseases.
  • the present invention provides an antithrombotic, or a method for preventing and/or treating a thrombotic disease, comprising administering the aforementioned composition or the aforementioned combined product to an individual in need thereof.
  • the above-mentioned thrombotic diseases are selected from myocardial infarction, ischemic cerebral infarction, and venous thromboembolism and other related diseases caused by blood clots.
  • the invention also discloses an antithrombotic red meat apple and cherry plum combination product and a preparation method thereof, including product forms such as fresh fruit, fruit juice, jam, freeze-dried powder, tablet candy, oral liquid, capsule and the like. Animal experiments show that this series of products has a good synergistic antithrombotic effect.
  • the composition of the present invention can also be used to prepare medicines such as traditional Chinese medicines and Chinese patent medicines.
  • the present application also provides a combination product comprising a first product and a second product individually packaged, the first product being a red meat apple and cherry plum combination product, and the second product being Antithrombotic drugs (eg aspirin, clopidogrel, heparin, warfarin, etc.).
  • a combination product comprising a first product and a second product individually packaged, the first product being a red meat apple and cherry plum combination product, and the second product being Antithrombotic drugs (eg aspirin, clopidogrel, heparin, warfarin, etc.).
  • “individual” includes humans or non-human animals.
  • Exemplary human subjects include human subjects (referred to as patients) or normal subjects with a disease (eg, a disease described herein).
  • “Non-human animals” in the present invention include all vertebrates such as non-mammals (eg fish, birds, amphibians, reptiles) and mammals such as non-human primates, livestock and/or domesticated animals (eg mice, sheep, dogs, cats, cows, pigs, etc.).
  • cherry plum extract or “red flesh apple extract” refers to obtaining and enriching cherry plum or red flesh apple through physical and/or chemical extraction and separation process using cherry plum or red flesh apple as raw material
  • the product formed by one or more active ingredients in the product can be used as the raw material or auxiliary material of food (such as ordinary food or health food) or medicine.
  • Exemplary extraction methods include, but are not limited to: leaching extraction, enzymatic hydrolysis ultrasonic extraction, ultra-high pressure extraction and subcritical extraction.
  • the red flesh apple and cherry plum extracts of the present invention are obtained by leaching extraction (eg, water leaching extraction or alcohol leaching extraction).
  • leaching extraction eg, water leaching extraction or alcohol leaching extraction.
  • the leaching extraction comprises the steps of:
  • Step 1 The red-fleshed apples and cherry plums are respectively deseeded or pitted and beaten to obtain pulp directly, or the juice and pomace are separated; in certain embodiments, gauze (such as 100-300 mesh gauze, 200-mesh gauze) filter to separate out fruit juice and pomace;
  • gauze such as 100-300 mesh gauze, 200-mesh gauze
  • Step 2 Mix the pulp or pomace with an extraction medium (such as water or ethanol), and extract (such as 30-100 min, such as 60 min) at room temperature or under heating (such as 10-100° C., such as 50° C.),
  • the pulp or pomace extract is obtained by filtration; in certain embodiments, the pulp or pomace is mixed with the extraction medium in a weight ratio of 1:(2-10) (eg, 1:5); in certain embodiments , the ethanol is 50%-95% ethanol;
  • Step 3 Concentrating the pulp extract or the combined solution of the fruit juice and the pomace extract to obtain an extract of red-fleshed apple and cherry plum; in some embodiments, concentration is performed by rotary evaporation; in some In embodiments, the temperature of the rotary evaporation is 30-60°C, eg, 50°C;
  • Step 4 Mix the extracted red meat apple and cherry plum extracts in different proportions (weight ratios) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10: 1) Prepare a composition, or in step 1, mix red-meat apple and cherry plum raw materials in different proportions, and then extract.
  • the red-fleshed apple and cherry plum extracts of the present invention are obtained by water extraction, and in certain embodiments, the water extraction and extraction comprises the following steps:
  • Step 1 Deseed or pit the red-fleshed apples and cherry plums, respectively, and beat the pulp to obtain pulp directly, or filter the juice with 100-300-mesh gauze to obtain red-fleshed apple and cherry plum juice, and separate the juice and the pomace;
  • the mesh of the gauze is 200 meshes;
  • Step 2 Red-fleshed apple and cherry plum pulp or pomace are mixed with water at a ratio of 1: (2-10) to water, leaching at 10-100°C for 30-100min, and filtering to obtain pulp or pomace extract ;
  • the ratio of solid to liquid is 1:5; in certain embodiments, the extraction temperature is 50°C; in certain embodiments, the extraction time is 60 min;
  • Step 3 Red-fleshed apple and cherry plum pulp extract or the combined solution of fruit juice and pomace extract, concentrated by rotary evaporation at 30-60 ° C, to obtain red-fleshed apple and cherry plum water extract;
  • the rotary evaporation temperature is 50°C;
  • Step 4 Mix the extracted red meat apple and cherry plum water extracts according to different ratios (weight ratios) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1 , 10:1) is prepared into a composition, or in step 1, the raw materials of red meat apple and cherry plum are mixed according to the aforementioned different ratios, and then extracted.
  • the red-fleshed apple and cherry plum extracts of the present invention are obtained by alcohol extraction, and in certain embodiments, the alcohol extraction comprises the following steps:
  • Step 1 Deseed or pit the red-fleshed apples and cherry plums, respectively, and beat the pulp to obtain pulp directly, or filter the juice with 100-300-mesh gauze to obtain red-fleshed apple and cherry plum juice, and separate the juice and the pomace;
  • the mesh of the gauze is 200 meshes;
  • Step 2 Red-fleshed apple and cherry plum pulp or pomace are mixed with 50%-95% ethanol according to the ratio of material to liquid 1: (2-10), leaching at 10-100 °C for 30-100min, and filtering to obtain fruit pulp or pomace extract; in certain embodiments, the ratio of solid to liquid is 1:5; in certain embodiments, the concentration of ethanol used is 50%; in certain embodiments, leaching
  • the extraction temperature is 50°C; in some embodiments, the extraction time is 60min;
  • Step 3 The red-fleshed apple and cherry plum pulp extract or the combined solution of fruit juice and pomace extract is concentrated by rotary evaporation at 30-60°C, and the pulp extract is directly concentrated to obtain red-flesh apple and cherry plum alcohol leaching Extract the extract; in certain embodiments, the rotary evaporation temperature is selected to be 50°C; optionally, the extract is subjected to a drying treatment to remove residual ethanol therein;
  • Step 4 Mix the red-fleshed apple and cherry plum alcohol extracts obtained by extraction according to different ratios (weight ratios) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1 , 10:1) is prepared into a composition, or in step 1, the raw materials of red meat apple and cherry plum are mixed according to the aforementioned different ratios, and then extracted.
  • the red flesh apple and cherry plum extracts of the present invention are obtained by enzymatic ultrasonic extraction (e.g., enzymatic ultrasonic water extraction or enzymatic ultrasonic alcohol extraction).
  • enzymatic ultrasonic extraction e.g., enzymatic ultrasonic water extraction or enzymatic ultrasonic alcohol extraction.
  • the enzymatic hydrolysis ultrasonic extraction comprises the following steps:
  • Step 1 The red-fleshed apples and cherry plums are respectively deseeded or pitted and beaten to obtain pulp directly, or the juice and pomace are separated; in certain embodiments, gauze (such as 100-300 mesh gauze, 200-mesh gauze) filter to separate out fruit juice and pomace;
  • gauze such as 100-300 mesh gauze, 200-mesh gauze
  • Step 2 Mix the pulp or pomace with an extraction medium (eg, water or ethanol) and cellulase, and extract under heating conditions (eg, 30-50°C, eg, 45°C) (eg, 30-100 min, eg, 60 min) , and then ultrasonic (such as ultrasonic 20-60min, such as ultrasonic 30min) under ultrasonic conditions (such as ultrasonic power of 100-500W), filter to obtain pulp or pomace extract;
  • the addition of cellulase The amount (based on the weight of the pomace or pulp) is 0.2%-1% (eg 0.5%); in certain embodiments, the pulp or pomace to extraction medium is 1:(2-10) (eg 1 : 5) weight ratio mixing;
  • the ethanol is 50%-95% ethanol;
  • Step 3 Concentrating the red-fleshed apple and cherry plum pulp extract or the combined solution of fruit juice and pomace extract to obtain an enzymatic hydrolysis ultrasonic extract of red-fleshed apple and cherry plum; in certain embodiments, by rotating Concentrating by evaporation; in certain embodiments, the temperature of rotary evaporation is 30-60°C, such as 50°C;
  • Step 4 Mix the extracted red meat apple and cherry plum enzymolysis ultrasonic extract according to different ratios (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1 , 10:1) is prepared into a composition, or in step 1, the raw materials of red meat apple and cherry plum are mixed according to the aforementioned different ratios, and then extracted.
  • the red-fleshed apple and cherry plum extracts of the present invention are obtained by enzymatic hydrolysis ultrasonic water extraction, and in certain embodiments, the enzymatic hydrolysis ultrasonic water extraction comprises the following steps:
  • Step 1 Deseed or pit the red-fleshed apples and cherry plums, respectively, and beat the pulp to obtain pulp directly, or filter the juice with 100-300-mesh gauze to obtain red-fleshed apple and cherry plum juice, and separate the juice and the pomace;
  • the mesh of the gauze is 200 meshes;
  • Step 2 Mix red-fleshed apple and cherry plum pulp or pomace with water at a ratio of 1:(2-10) to water, and add 0.2%-1% (based on the weight of pomace or pulp) cellulose Enzyme, 30-50°C water bath extraction for 30-100min, 100-500W ultrasonic for 20-60min, filtration to obtain pulp or pomace extract; in certain embodiments, the ratio of material to liquid is 1:5; in certain embodiments In the scheme, the amount of cellulase added is 0.5%; in certain embodiments, the temperature of the water bath is 45°C; in certain embodiments, the extraction time is 60 min; in certain embodiments, the ultrasonic power is 500W; In certain embodiments, the sonication time is 30 min;
  • Step 3 The combined solution of red-fleshed apple and cherry plum pulp or juice and pomace extract is concentrated by rotary evaporation at 30-60 °C to obtain the red-fleshed apple and cherry plum enzymatic hydrolysis ultrasonic extraction water extract;
  • the rotary evaporation temperature is 50°C;
  • Step 4 Mix the extracted red meat apple and cherry plum enzymolysis ultrasonic water extract according to different ratios (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5: 1. 10:1) to prepare a composition, or in step 1, red-fleshed apple and cherry plum raw materials are mixed according to the aforementioned different ratios, and then extracted.
  • the red-fleshed apple and cherry plum extracts of the present invention are obtained by enzymatic hydrolysis ultrasonic alcohol extraction, and in certain embodiments, the enzymatic hydrolysis ultrasonic alcohol extraction comprises the following steps:
  • Step 1 Deseed or pit the red-fleshed apples and cherry plums, respectively, and beat the pulp to obtain pulp directly, or filter the juice with 100-300-mesh gauze to obtain red-fleshed apple and cherry plum juice, and separate the juice and the pomace;
  • the mesh of the gauze is 200 meshes;
  • Step 2 Mix red-fleshed apple and cherry plum pulp or pomace with 50%-95% ethanol according to the ratio of material to liquid 1:(2-10), add 0.2%-1% (by weight of pomace or pulp) Be benchmark) cellulase, 30-50 °C of water bath extraction 30-100min, 100-500W ultrasonic 20-60min, filter to obtain pulp or pomace extract;
  • the material-to-liquid ratio is selected 1: 5; in certain embodiments, the concentration of ethanol used is 50%; in certain embodiments, the amount of cellulase added is 0.5%; in certain embodiments, the temperature of the water bath is 45°C;
  • the extraction time is 60min; in some embodiments, the ultrasonic power is 500W; in some embodiments, the ultrasonic time is 30min;
  • Step 3 red-fleshed apple and cherry plum pulp extract or the combined solution of fruit juice and pomace extract, concentrated by rotary evaporation at 30-60° C. to obtain red-fleshed apple and cherry plum enzymatic hydrolysis ultrasonic extraction alcohol extract;
  • the rotary evaporation temperature is selected at 50°C; optionally, the extract is subjected to evaporation treatment to remove residual ethanol therein;
  • Step 4 Mix the extracted red meat apple and cherry plum enzymolysis ultrasonic alcohol extract according to different ratios (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5: 1. 10:1) to prepare a composition, or in step 1, red-fleshed apple and cherry plum raw materials are mixed according to the aforementioned different ratios, and then extracted.
  • the red flesh apple and cherry plum extracts of the present invention are obtained by ultra-high pressure extraction (eg, ultra-high pressure water extraction or ultra-high pressure alcohol extraction).
  • ultra-high pressure refers to pressures in excess of 100 MPa (equivalent to 1000 atmospheres).
  • the ultra-high pressure extraction comprises the steps of:
  • Step 1 The red-fleshed apples and cherry plums are respectively deseeded or pitted and beaten to obtain pulp directly, or the juice and pomace are separated; in certain embodiments, gauze (such as 100-300 mesh gauze, 200-mesh gauze) filter to separate out fruit juice and pomace;
  • gauze such as 100-300 mesh gauze, 200-mesh gauze
  • Step 2 Mix the pulp or pomace with an extraction medium (such as water or ethanol), process (such as 3-10 min, such as 5 min) under ultra-high pressure (such as 200-600 MPa, such as 400 MPa), and filter to obtain the fruit.
  • pulp or pomace extract in certain embodiments, the pulp or pomace is mixed with the extraction medium in a weight ratio of 1:(2-10) (eg, 1:5); in certain embodiments, the Ethanol is 50%-95% ethanol;
  • Step 3 Concentrating the combined solution of red-fleshed apple and cherry plum pulp or juice and pomace extract to obtain an ultra-high pressure extract of red-fleshed apple and cherry plum; in certain embodiments, concentrated by rotary evaporation ; In certain embodiments, the temperature of the rotary evaporation is 30-60°C, such as 50°C;
  • Step 4 Mix the extracted red meat apple and cherry plum ultra-high pressure extracts according to different ratios (weight ratios) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) Prepare a composition, or in step 1, mix red-meat apple and cherry plum raw materials in different proportions, and then extract.
  • the red-fleshed apple and cherry plum extracts of the present invention are obtained by ultra-high pressure water extraction, and in certain embodiments, the ultra-high pressure water extraction comprises the following steps:
  • Step 1 Deseed or pit the red-fleshed apples and cherry plums, respectively, and beat the pulp to obtain pulp directly, or filter the juice with 100-300-mesh gauze to obtain red-fleshed apple and cherry plum juice, and separate the juice and the pomace;
  • the mesh of the gauze is 200 meshes;
  • Step 2 Red-fleshed apple and cherry plum pulp or pomace are mixed with water according to a material-to-liquid ratio of 1: (2-10), treated with ultra-high pressure 200-600MPa for 3-10min, and filtered to obtain pulp or pomace extract;
  • the ratio of material to liquid is 1:5; in certain embodiments, the ultra-high pressure is 400MPa; in certain embodiments, the ultra-high pressure treatment time is 5min;
  • Step 3 Red-fleshed apple and cherry plum pulp extract or the combined solution of fruit juice and pomace extract, concentrated by rotary evaporation at 30-60 ° C, to obtain red-fleshed apple and cherry plum ultra-high pressure extraction water extract;
  • the rotary evaporation temperature is selected to be 50 °C;
  • Step 4 Mix the extracted red meat apple and cherry plum ultra-high pressure water extracts according to different ratios (weight ratios) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1 , 10:1) is prepared into a composition, or in step 1, the raw materials of red meat apple and cherry plum are mixed according to the aforementioned different ratios, and then extracted.
  • the red-fleshed apple and cherry plum extracts of the present invention are obtained by ultra-high pressure alcohol extraction, and in certain embodiments, the ultra-high pressure alcohol extraction comprises the following steps:
  • Step 1 Deseed or pit the red-fleshed apples and cherry plums, respectively, and beat the pulp to obtain pulp directly, or filter the juice with 100-300-mesh gauze to obtain red-fleshed apple and cherry plum juice, and separate the juice and the pomace;
  • the mesh of the gauze is 200 meshes;
  • Step 2 Red-fleshed apple and cherry plum pulp or pomace are mixed with 50%-95% ethanol according to the ratio of material to liquid 1: (2-10), treated with ultra-high pressure 200-600MPa for 3-10min, and filtered to obtain pulp Or pomace extract; in certain embodiments, the ratio of material to liquid is 1:5; in certain embodiments, the ultra-high pressure is 400MPa; in certain embodiments, the ultra-high pressure treatment time is selected 5min; In some embodiments, the ethanol is 50% ethanol;
  • Step 3 Red-fleshed apple and cherry plum pulp extract or the combined solution of fruit juice and pomace extract, concentrated by rotary evaporation at 30-60°C, to obtain red-fleshed apple and cherry plum ultra-high pressure extraction alcohol extract;
  • the rotary evaporation temperature is selected as 50°C; optionally, the extract is subjected to evaporation treatment to remove residual ethanol therein;
  • Step 4 Mix the extracted red meat apple and cherry plum ultra-high pressure alcohol extracts according to different ratios (weight ratios) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1 , 10:1) is prepared into a composition, or in step 1, the raw materials of red meat apple and cherry plum are mixed according to the aforementioned different ratios, and then extracted.
  • red flesh apple and cherry plum extracts are obtained by subcritical extraction (eg, subcritical water extraction or subcritical alcohol extraction).
  • the subcritical extraction comprises the steps of:
  • Step 1 The red-fleshed apples and cherry plums are respectively deseeded or pitted and beaten to obtain pulp directly, or the juice and pomace are separated; in certain embodiments, gauze (such as 100-300 mesh gauze, 200-mesh gauze) filter to separate out fruit juice and pomace;
  • gauze such as 100-300 mesh gauze, 200-mesh gauze
  • Step 2 Mix the pulp or pomace with an extraction medium (such as water or ethanol), perform a subcritical extraction treatment (such as treatment for 10-50 min, such as 30 min), and filter to obtain the pulp or pomace extract; in certain embodiments, the pulp or pomace is mixed with the extraction medium in a weight ratio of 1:(2-10) (eg 1:5); in certain embodiments, the ethanol is 50%-95% ethanol; In some embodiments, the treatment temperature for subcritical extraction is 100-150°C (eg, 110°C);
  • Step 3 Concentrating the combined solution of pulp or fruit juice and pomace extract to obtain subcritical extracts of red-fleshed apples and cherry plums; in certain embodiments, concentrated by rotary evaporation; in certain embodiments , the temperature of rotary evaporation is 30-60°C, such as 50°C;
  • Step 4 Mix the extracted red meat apple and cherry plum sub-critical extracts according to different ratios (weight ratios) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) Prepare a composition, or in step 1, mix red-meat apple and cherry plum raw materials in different proportions, and then extract.
  • the red flesh apple and cherry plum extracts of the present invention are obtained by subcritical water extraction, in certain embodiments, the subcritical water extraction comprises the following steps:
  • Step 1 Deseed or pit the red-fleshed apples and cherry plums, respectively, and beat the pulp to obtain pulp directly, or filter the juice with 100-300-mesh gauze to obtain red-fleshed apple and cherry plum juice, and separate the juice and the pomace;
  • the mesh of the gauze is 200 mesh;
  • Step 2 Red-fleshed apple and cherry plum pulp or pomace are mixed with water according to the ratio of material to liquid 1: (2-10), subcritically extracted for 10-50min, the treatment temperature is 100-150 °C, filtered to obtain pulp or Pomace extract; in certain embodiments, the ratio of solid to liquid is 1:5; in certain embodiments, the treatment temperature is 110°C; in certain embodiments, the treatment time is 30min;
  • Step 3 Red-fleshed apple and cherry plum pulp extract or the combined solution of fruit juice and pomace extract, concentrated by rotary evaporation at 30-60°C, to obtain red-fleshed apple and cherry plum subcritical extraction water extract;
  • the rotary evaporation temperature is selected to be 50 °C;
  • Step 4 Mix the extracted red-fleshed apple and cherry plum subcritical water extracts according to different ratios (weight ratios) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1 , 10:1) is prepared into a composition, or in step 1, the raw materials of red meat apple and cherry plum are mixed according to the aforementioned different ratios, and then extracted.
  • the red flesh apple and cherry plum extracts of the present invention are obtained by subcritical alcohol extraction, and in certain embodiments, the subcritical alcohol extraction comprises the following steps:
  • Step 1 Deseed or pit the red-fleshed apples and cherry plums, respectively, and beat the pulp to obtain pulp directly, or filter the juice with 100-300-mesh gauze to obtain red-fleshed apple and cherry plum juice, and separate the juice and the pomace;
  • the mesh of the gauze is 200 mesh;
  • Step 2 Red-fleshed apple and cherry plum pulp or pomace are mixed with 50%-95% ethanol according to the material-to-liquid ratio of 1: (2-10), subcritically extracted for 10-50min, and the processing temperature is 100-150°C, Filtration to obtain pulp or pomace extract; in certain embodiments, the ratio of solid to liquid is 1:5; in certain embodiments, the treatment temperature is 110 ° C; in certain embodiments, the treatment time is 30min ;
  • Step 3 Red-fleshed apple and cherry plum pulp extract or the combined solution of fruit juice and pomace extract, concentrated by rotary evaporation at 30-60°C, to obtain red-fleshed apple and cherry plum subcritical extraction alcohol extract;
  • the rotary evaporation temperature is selected as 50°C; optionally, the extract is subjected to evaporation treatment to remove residual ethanol therein;
  • Step 4 Mix the red-fleshed apple and cherry plum subcritical alcohol extracts according to different ratios (weight ratios) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1 , 10:1) is prepared into a composition, or in step 1, the raw materials of red meat apple and cherry plum are mixed according to the aforementioned different ratios, and then extracted.
  • the product form of the composition of the present invention can be fresh fruit, fruit juice, jam, fruit powder (such as freeze-dried powder), candy (such as tablet candy), oral liquid, capsule, tablet, dried fruit, preserved fruit, Fruit wine or pastries.
  • the product form of the composition of the present invention can be medicine, general food or health food.
  • the composition can be used as the main raw material, for example, accounting for more than 30wt%, more than 40wt%, more than 50wt%, more than 60wt%, more than 70wt%, more than 80wt%, more than 90wt% or 100wt% of the total raw materials;
  • the product may also contain other ingredients such as water, sweeteners, or other kinds of fruit or extracts thereof.
  • red meat apple and cherry plum products eg, fresh fruit, juices, jams, lyophilized powders, compressed candies, oral liquids, capsules.
  • red flesh apple and cherry plum fresh fruit can be obtained by a method comprising the steps of:
  • Step 1 Harvest red-fleshed apples and cherry plums from 1 month before maturity to when they are fully mature, and choose to pick fruits on sunny days;
  • Step 2 Pick and remove rotten fruit or fruit with poor color
  • Step 3 Refrigerate storage after harvest
  • Step 4 Combine red-fleshed apples and fresh cherry plums in different ratios (weight ratios) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1).
  • the obtained red flesh apple and cherry plum fresh fruit can be eaten directly.
  • red meat apple and cherry plum juices can be obtained by a method comprising the steps of:
  • Step 1 Deseed or pit the red-fleshed apples and cherry plums separately;
  • Step 2 filter the slurry (for example, filter with 100-300 mesh gauze) to obtain red-meat apple and cherry plum juice cloudy juice; in certain embodiments, the mesh of the gauze is 200 mesh;
  • Step 3 Mix red meat apple and cherry plum juice in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) to prepare into a composition, or in step 1, the raw materials of red meat apple and cherry plum are mixed according to the aforementioned different proportions, and then beating is performed to prepare juice.
  • red meat apple and cherry plum juices can also be obtained by a method comprising the steps of:
  • Step 1 Deseed or pit the red-fleshed apples and cherry plums separately;
  • Step 2 filter the slurry (for example, filter with 100-300 mesh gauze), add pectinase enzymatic hydrolysis and filter; make red meat apple and cherry plum juice clear juice; in certain embodiments, the mesh of the gauze The number is 200 meshes;
  • Step 3 Mix red meat apple and cherry plum juice according to different ratios (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) to prepare The composition, or in step 1, the raw materials of red meat apple and cherry plum are mixed according to the aforementioned different proportions, and then beating is performed to prepare juice.
  • red meat apple and cherry plum jam can be obtained by a method comprising the steps of:
  • Step 1 Deseed or pit the red-fleshed apples and cherry plums separately;
  • Step 2 The pulp is boiled, heated and concentrated to make red meat apple and cherry plum jam
  • Step 3 Mix red meat apple and cherry plum jam in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) to prepare The composition, or in step 1, the raw materials of red meat apple and cherry plum are mixed according to the aforementioned different proportions, and then beating is performed to prepare jam.
  • red meat apple and cherry plum lyophilized powder can be obtained by a method comprising the steps of:
  • Step 1 Deseed or pit the red-fleshed apples and cherry plums separately
  • Step 2 Freeze-dry the fruit pulp to make the fruit pulp freeze-dried powder
  • Step 3 Mix red meat apple and cherry plum freeze-dried powder in different ratios (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1)
  • the composition is prepared, or in step 1, the raw materials of red meat apple and cherry plum are mixed according to the aforementioned different proportions, and then the lyophilized powder is prepared by beating.
  • red meat apple and cherry plum lyophilized powder can also be obtained by a method comprising the steps of:
  • Step 1 Deseed or pit the red-fleshed apples and cherry plums separately;
  • Step 2 filter the slurry (for example, filter with 100-300 mesh gauze) to obtain red meat apple and cherry plum juice; in certain embodiments, the mesh of the gauze is 200 mesh;
  • Step 3 freeze-dry the fruit juice to make fruit juice freeze-dried powder
  • Step 4 Mix red meat apple and cherry plum freeze-dried powder in different ratios (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1)
  • the composition is prepared, or in step 1, the raw materials of red meat apple and cherry plum are mixed according to the aforementioned different proportions, and then the lyophilized powder is prepared by beating.
  • red meat apple and cherry plum tableted candies can be obtained by a method comprising the steps of:
  • Step 1 Deseed or pit the red-fleshed apples and cherry plums separately;
  • Step 2 Freeze-dry the fruit pulp to make the fruit pulp freeze-dried powder
  • Step 3 Compress the fruit pulp freeze-dried powder and accessories to make tablet candy
  • Step 4 Mix the red-fleshed apple and cherry plum tablet candies in different ratios (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1)
  • the composition is prepared, or in step 1, the raw materials of red-meat apple and cherry plum are mixed according to the aforementioned different proportions, and then beating is performed to prepare a tablet candy.
  • red-fleshed apple and cherry plum tableted candies can also be obtained by a method comprising the steps of:
  • Step 1 Deseed or pit the red-fleshed apples and cherry plums separately;
  • Step 2 filter the slurry (for example, filter with 100-300 mesh gauze) to obtain red meat apple juice; in some embodiments, the mesh number of the gauze is 200 mesh;
  • Step 3 freeze-dry the fruit juice to make fruit juice freeze-dried powder
  • Step 4 Compress the fruit juice freeze-dried powder and accessories into tablet candies.
  • Step 5 Mix the red-fleshed apple and cherry plum candies in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1)
  • the composition is prepared, or in step 1, the raw materials of red-meat apple and cherry plum are mixed according to the aforementioned different proportions, and then beating is performed to prepare a tablet candy.
  • red meat apple and cherry plum oral liquid can be obtained by a method comprising the steps of:
  • Step 1 Preparation of red meat apple and cherry plum extracts in different ratios (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) , obtained by leaching extraction, enzymatic hydrolysis ultrasonic extraction, ultra-high pressure extraction or subcritical extraction, the steps are the same as above);
  • Step 2 sterilizing the extracts of red-fleshed apples and cherry plums to obtain red-flesh apple oral liquid.
  • red meat apple and cherry plum oral liquid can also be obtained by a method comprising the steps of:
  • Step 1 Preparation of red meat apple and cherry plum extracts in different ratios (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) , obtained by leaching extraction, enzymatic hydrolysis ultrasonic extraction, ultra-high pressure extraction or subcritical extraction, the steps are the same as above);
  • Step 2 adding pectinase enzymatic hydrolysis and filtering to prepare a clear juice extract composition
  • Step 3 sterilizing the extracts of red meat apple and cherry plum juice to obtain red meat apple oral liquid.
  • red meat apple and cherry plum capsules can be obtained by a method comprising the steps of:
  • Step 1 Deseed or pit the red-fleshed apples and cherry plums separately;
  • Step 2 Freeze-dry the fruit pulp to make the fruit pulp freeze-dried powder
  • Step 3 Mix red meat apple and cherry plum freeze-dried powder in different ratios (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) Prepare a composition, or in step 1, mix red-meat apples and cherry plum raw materials in different proportions, and then beating to prepare freeze-dried powder;
  • Step 4 Pack the lyophilized fruit pulp into the capsule shell to make capsules.
  • red meat apple and cherry plum capsules can also be obtained by a method comprising the steps of:
  • Step 1 Deseed or pit the red-fleshed apples and cherry plums separately;
  • Step 2 filter the slurry (for example, filter with 100-300 mesh gauze) to obtain red meat apple and cherry plum juice; in certain embodiments, the mesh of the gauze is 200 mesh;
  • Step 3 freeze-dry the fruit juice to make fruit juice freeze-dried powder
  • Step 4 Mix red meat apple and cherry plum freeze-dried powder in different ratios (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) Prepare a composition, or in step 1, mix red-meat apples and cherry plum raw materials in different proportions, and then beating to prepare freeze-dried powder;
  • Step 5 Pack the fruit juice freeze-dried powder into the capsule shell to make capsules.
  • the present invention selects natural red meat apple and cherry plum, and the prepared red meat apple and cherry plum combination product has good antithrombotic effect, and the effect of the combination of the two is better than that of using red meat apple or cherry plum alone, and has synergistic effect. effect.
  • the effect of the combination of the composition and the drug is better than that of the composition or the drug alone, and there is a certain synergistic effect.
  • the red-fleshed apple and cherry plum products described in this embodiment refer to the fresh fruit of red-fleshed apple and cherry plum.
  • Red-fleshed apples (Hongxun No. 1) and cherry plums (black fruit) are harvested from 1 month before maturity to when they are fully mature.
  • priority is given to picking fruits on sunny days, and rotten fruits or fruits with poor color are selected and removed.
  • the obtained red-fleshed apples and cherry plums are combined in different ratios (weight ratios) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1), and the fresh fruits can be directly edible.
  • the red meat apple and cherry plum products described in this example refer to the red meat apple and cherry plum juice cloudy juice.
  • Red-fleshed apples (Hongxun No. 1) and cherry plums (black fruit) were harvested and seeded or pitted, then beaten, and the juice was filtered with 200-mesh gauze to obtain red-fleshed apple and cherry plum juice.
  • the red meat apple and cherry plum cloudy juice are mixed in different ratios (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) to prepare a composition , or mix the raw materials of red meat apples and cherry plums according to the aforementioned different proportions, and then beating to prepare juice.
  • the red meat apple and cherry plum products described in this embodiment refer to the red meat apple and cherry plum juice clear juice.
  • Red-fleshed apples (Hongxun No. 1) and cherry plums (black fruit) were harvested and seeded or pitted, then beaten, and the juice was filtered with 200-mesh gauze to obtain red-fleshed apple and cherry plum juice.
  • Red meat apple and cherry plum juice are mixed according to different ratios (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) to prepare a composition, Or firstly, the raw materials of red-meat apple and cherry plum are mixed according to the aforementioned different proportions, and then the juice is prepared by beating.
  • red meat apple and cherry plum products described in this example refer to red meat apple and cherry plum jam.
  • Red-fleshed apples (Hongxun No. 1) and cherry plums (black fruit) are seeded or pitted, beaten, boiled and heated to make a thick jam.
  • the red meat apple and cherry plum jam are mixed in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) to prepare a composition, Or firstly, the raw materials of red meat apple and cherry plum are mixed according to the aforementioned different proportions, and then the jam is prepared by beating.
  • the red meat apple and cherry plum products described in this example refer to the freeze-dried powder of red meat apple and cherry plum.
  • Red-fleshed apples (Hongxun No. 1) and cherry plums (black fruit) are seeded or pitted, beaten and then freeze-dried to produce freeze-dried powder.
  • Red meat apple and cherry plum freeze-dried powder are mixed in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) to prepare a combination or mix the raw materials of red meat apples and cherry plums according to the aforementioned different proportions, and then beating to prepare freeze-dried powder.
  • red-fleshed apple and cherry plum products described in this example refer to red-fleshed apple and cherry plum pressed candies.
  • Red-fleshed apples (Hongxun No. 1) and cherry plums (black fruit) are seeded or pitted and beaten, freeze-dried to make pulp freeze-dried powder, and the pulp freeze-dried powder and accessories are compressed to make tablet candy.
  • the red meat apple and cherry plum pressed candies are mixed in different proportions (weight ratio) (1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1) to prepare a combination
  • the raw materials of red meat apples and cherry plums are mixed according to the aforementioned different proportions, and then beaten to prepare tablet candies.
  • the red-fleshed apple and cherry plum products described in this example refer to the water-extracted oral liquid of red-fleshed apple and cherry plum.
  • Red-fleshed apples Haongxun No. 1
  • cherry plums black fruit
  • the red-fleshed apple and cherry plum products described in this example refer to the water-extracted oral liquid of red-fleshed apple (red love) and cherry plum (red fruit).
  • the preparation steps are the same as those in Example 7.
  • the red-fleshed apple and cherry plum products described in this example refer to the alcohol extraction oral liquid of red-fleshed apple (Hongxun No. 1) and cherry plum (black fruit).
  • red-fleshed apple and cherry plum products described in this embodiment refer to the oral solution of red-fleshed apple and cherry plum enzymatic hydrolysis ultrasonic water extraction.
  • Red-fleshed apples Haongxun No. 1
  • cherry plums black fruit
  • red-fleshed apple and cherry plum products described in this example refer to the oral liquid extracted from red-fleshed apple (Hongxun No. 3) and cherry plum (red fruit) by enzymatic hydrolysis and ultrasonic water extraction.
  • the preparation steps are the same as those in Example 10.
  • red-fleshed apple and cherry plum products described in this example refer to the red-fleshed apple (Hongxun No. 1) and the cherry plum (black fruit) enzymatic hydrolysis ultrasonic alcohol extraction oral liquid.
  • the red meat apple and cherry plum products described in this embodiment refer to the ultra-high pressure water extraction oral liquid of red meat apple and cherry plum.
  • Red-fleshed apples Haongxun No. 1
  • cherry plums black fruit
  • red-fleshed apple and cherry plum products described in this example refer to the ultra-high pressure water extraction oral liquid of red-fleshed apple (Hongxun No. 6) and cherry plum (red fruit).
  • the preparation steps are the same as those in Example 13.
  • the red-fleshed apple and cherry plum products described in this example refer to the ultra-high pressure alcohol extraction oral liquid of red-fleshed apple (Hongxun No. 1) and cherry plum (black fruit).
  • Example 13 Refer to the steps (1)-(5) of Example 13, wherein the fruit pulp is added 50% according to the ratio of material to liquid 1:5.
  • the alcoholic extract was treated with ethanol to evaporate.
  • the red meat apple and cherry plum products described in this example refer to the subcritical water extraction oral liquid of red meat apple and cherry plum.
  • Red-fleshed apples Haongxun No. 1
  • cherry plums black fruit
  • the zebrafish larvae with embryonic development phase of 2dpf were selected and divided into model group, blank group (water), solvent control group, positive drug group (clopidogrel) and sample group.
  • Model group, solvent control group, positive drug group and sample group were modeled by adding ferric chloride (200 ⁇ mol/L).
  • the positive drug group was given clopidogrel, and the sample group was given the test sample.
  • the zebrafish embryos in each group were placed in a 28°C incubator to continue culturing. After 24 hours of drug action, zebrafish erythrocytes in each group were stained and photographed.
  • Use the image analysis software IPP to analyze the staining intensity of cardiac erythrocytes, record the IOD value, and calculate the thrombus inhibition rate of the corresponding drug according to the formula:
  • Thrombus inhibition rate (%) (IOD drug group-IOD model group)/(IOD solvent group-IOD model group) ⁇ 100
  • the test results are shown in Table 1 below.
  • the samples of the red-fleshed apple group and the cherry plum group alone are the red-fleshed apple enzymolysis ultrasonic water extract and the cherry plum enzymolysis ultrasonic water extract obtained in step (3) of Example 10, respectively.
  • the samples of the red-fleshed apple and cherry plum composition group are the compositions of each weight ratio prepared in step (4) of Example 10.
  • each group is shown with a single underline, wherein: 20 ⁇ g/mL or 10 ⁇ g/mL represents the concentration of the positive drug dispersed in water; 0.002% represents the red meat apple enzymatic hydrolysis ultrasonic water extract, or cherry plum enzymatic hydrolysis The concentration of ultrasonic water extract, or the composition consisting of red meat apple enzymolysis ultrasonic water extract and cherry plum enzymolysis ultrasonic water extract in different weight ratios dispersed in water.
  • the thrombus inhibition rate of the red-fleshed apple and cherry plum composition (10:1-1:10) is higher than the value when used alone, and the calculated q values are all greater than 1.15, indicating that there is a synergistic effect.
  • the red-fleshed apple and cherry plum composition has an additive effect (0.85 ⁇ q ⁇ 1.15) when used in combination with the positive drug, and the antithrombotic effect is better than the effect when used alone.
  • mice were randomly divided into 4 groups: sample group, normal group, model group, positive drug group, 20 mice in each group.
  • the sample group, model group and positive drug group were given intragastric administration for 7 days to establish models.
  • the sample group and the positive drug group continued to be given by gavage, the positive drug group was given clopidogrel, and the sample group was given the test sample.
  • the dose of each group was 0.5ml, and the dose of each group was 20mg/kg.
  • the model group and the normal group were treated with water, and no positive drugs or samples to be tested were given. After 48 hours, the inhibitory effect of the sample on thrombus formation was evaluated by the ratio of the length of the thrombus formed in the tail of the mouse (the ratio of the black tail ratio to the total tail length).
  • Thrombus inhibition rate (%) (black tail rate in model group - black tail rate in drug group)/black tail rate in model group ⁇ 100
  • test results are shown in Table 2 below.
  • the samples of the red-fleshed apple group and the cherry plum group alone are the red-fleshed apple enzymolysis ultrasonic water extract and the cherry plum enzymolysis ultrasonic water extract obtained in step (3) of Example 10, respectively.
  • the samples of the red-fleshed apple and cherry plum composition group are the compositions of each weight ratio prepared in step (4) of Example 10.
  • the dose administered corresponding to each group is shown with a single underline.
  • the red-fleshed apple and cherry plum composition had an additive effect (0.85 ⁇ q ⁇ 1.15) when used in combination with a normal dose of the positive drug.
  • the antithrombotic effect of the low-dose positive drug is general.
  • the q value is greater than 1.15, which has a synergistic effect.
  • Drugs generally have side effects, and the red-fleshed apple and cherry plum composition combined with the drug has better effect, and at the same time, the dosage of the drug can be reduced, and the toxic and side effects of the drug can be alleviated.

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Abstract

L'invention concerne une composition de Malus neidzwetzkyana Dieck et Prunus cerasifera Ehrhart ayant une efficacité antithrombotique. Le rapport de poids entre Malus neidzwetzkyana Dieck et Prunus cerasifera Ehrhart vaut de 1:10 à 10:1. Les expériences menées sur des animaux comme le poisson zèbre et la souris montrent que la composition de Malus neidzwetzkyana Dieck et Prunus cerasifera Ehrhart présente des effets synergiques sur le plan de l'efficacité antithrombotique, et que l'efficacité antithrombotique de la composition est supérieure à celle de Malus neidzwetzkyana Dieck ou Prunus cerasifera Ehrhart utilisés seuls. Les formes de produits de la composition de Malus neidzwetzkyana Dieck et Prunus cerasifera Ehrhart comprennent le jus de fruits, la confiture de fruits, la poudre lyophilisée, les produits liquides administrés par voie orale, les capsules, les comprimés, etc. L'association de la composition de Malus neidzwetzkyana Dieck et Prunus cerasifera Ehrhart avec des médicaments présente également d'obtenir certains effets synergiques.
PCT/CN2021/133009 2020-11-26 2021-11-25 Composition de malus neidzwetzkyana dieck et de prunus cerasifera ehrhart, et son utilisation dans le traitement antithrombotique WO2022111561A1 (fr)

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