WO2023217115A1 - Composition de malus niedzwetzkyana dieck et de prunus cerasifera ehrhart et leur utilisation en termes de résistance à l'athérosclérose - Google Patents

Composition de malus niedzwetzkyana dieck et de prunus cerasifera ehrhart et leur utilisation en termes de résistance à l'athérosclérose Download PDF

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Publication number
WO2023217115A1
WO2023217115A1 PCT/CN2023/092926 CN2023092926W WO2023217115A1 WO 2023217115 A1 WO2023217115 A1 WO 2023217115A1 CN 2023092926 W CN2023092926 W CN 2023092926W WO 2023217115 A1 WO2023217115 A1 WO 2023217115A1
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Prior art keywords
red
cherry plum
fleshed
extraction
apple
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PCT/CN2023/092926
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English (en)
Chinese (zh)
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沈妍
李栋
胡柳
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浙江养生堂天然药物研究所有限公司
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Publication of WO2023217115A1 publication Critical patent/WO2023217115A1/fr

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
    • A23L21/10—Marmalades; Jams; Jellies; Other similar fruit or vegetable compositions; Simulated fruit products
    • A23L21/12—Marmalades; Jams; Jellies; Other similar fruit or vegetable compositions; Simulated fruit products derived from fruit or vegetable solids
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/736—Prunus, e.g. plum, cherry, peach, apricot or almond
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Definitions

  • the present invention relates to the field of food and medicine, and in particular to red-fleshed apple and cherry plum compositions and their application in anti-atherosclerosis.
  • Atherosclerosis is an inflammatory disease involving the arterial wall, characterized by the accumulation of lipids and inflammatory cells in the intima of large arteries. Atherosclerosis can cause atherosclerotic cardiovascular disease (ASCVD).
  • ASCVD diseases mainly include: acute coronary syndrome, history of myocardial infarction, stable or unstable angina, coronary revascularization (including interventional therapy and bypass surgery), other peripheral arterial diseases or revascularization
  • ASCVD After stroke or transient ischemic attack due to atherosclerosis, ASCVD has become the leading cause of death worldwide and seriously endangers health.
  • the incidence of atherosclerosis in my country has a significant upward trend, and it is more common in middle-aged and elderly people. Research shows that by 2020, 2 billion people worldwide will have carotid atherosclerosis. A survey involving 85,000 people in my country showed that 1/3 of the elderly have atherosclerosis.
  • Clinical drugs for the treatment of atherosclerosis mainly include: (1) hypolipidemic drugs: statins, ezetimibe, PCSK9 inhibitors, etc.; (2) antiplatelet drugs: aspirin, clopidogrel, etc.
  • the mechanism of AS is complex.
  • LDL-C low density lipoprotein cholesterol
  • Lipid-lowering drugs are the first choice drugs.
  • oxidative stress theory and inflammation theory regarding the mechanism of atherosclerosis.
  • Atherosclerosis requires a treatment plan under the guidance of a doctor and long-term medication. Different drugs will produce varying degrees of side effects on the human body, such as liver and muscle damage, gastrointestinal reactions, etc. Therefore, more natural and safe methods of intervention are needed.
  • Red-fleshed apple (Malus neidzwetzkyana Dieck) is a plant of the genus Malus of the Rosaceae family. Its fruit has red flesh and has very high potential utilization value in the research, processing and utilization of germplasm resources. There are many varieties of red-fleshed apples, with large differences in fruit size and color. my country, New Zealand, Japan, Switzerland and other countries have carried out red-fleshed apple breeding. Specifically, they include the Hongxun series from China’s Xinjiang, the Robert Crab from the United States, and the Japanese Japan’s Ruby Sweet, Switzerland’s Red Love, etc. The red-fleshed apples of Xinjiang play an important role in the origin of the red-fleshed Malus species.
  • Cherry plum (Prunus cerasifera Ehrhart), commonly known as wild sour plum, is a plant of the genus Prunus in the Rosaceae family. Wild cherry plums are listed as national level II key protected species. They are rare and endangered primitive wild fruit tree species resources in the world. They are sparsely distributed naturally around the world. The large and small Xigou in the Ili River Valley in Xinjiang is the world's largest concentration of wild cherry plums. . Cherry plum fruit has a long history of consumption in Xinjiang, my country. The diet of Kazakh and Mongolian herdsmen living in Daxigou, Tianshan Mountains is mainly beef and mutton. There are very few people suffering from hyperlipidemia and hypertension among them.
  • Cherry plums include many varieties. According to color, they mainly include black fruit, red fruit, purple fruit, purple-black fruit, yellow fruit, etc.
  • Red-fleshed apples and cherry plums are rich in functional components such as anthocyanins, polyphenols, and flavonoids, and have high development value.
  • current research is still relatively limited, mainly focusing on chemical composition and antioxidant aspects, and is still in the preliminary research stage.
  • red-fleshed apples or red-fleshed apple extracts
  • cherry plums or cherry plum extracts
  • the invention provides a composition comprising:
  • a first component the first component being a red-fleshed apple or a red-fleshed apple extract
  • the second component is cherry plum or cherry plum extract.
  • the weight ratio of the first component and the second component is 1:10-10:1 (such as 1:9-10:1, 1:8-10:1, 1: 7-10:1, 1:6-10:1, 1:5-10:1, 1:4-10:1, 1:3-10:1, 1:2-10:1, 1:1- 10:1, 1:10-9:1, 1:9-9:1, 1:8-9:1, 1:7-9:1, 1:6-9:1, 1:5-9: 1, 1:4-9:1, 1:3-9:1, 1:2-9:1, 1:1-9:1, 1:10-8:1, 1:9-8:1, 1:8-8:1, 1:7-8:1, 1:6-8:1, 1:5-8:1, 1:4-8:1, 1:3-8:1, 1: 2-8:1, 1:1-8:1, 1:10-7:1, 1:9-7:1, 1:8-7:1, 1:7-7:1, 1:6- 7:1, 1:5-7:1, 1:4-7:1, 1:3-7:1, 1:2-7:1, 1:1-7:1, 1:10-6: 1, 1:9-6:1, 1:8-6
  • the red-fleshed apple or red-fleshed apple extract is obtained by one of the following methods: fresh fruit, fresh fruit pulping or juice extraction, leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, macroporous resin Separation or other extraction methods.
  • the cherry plum or cherry plum extract is obtained by one of the following methods: fresh fruit, fresh fruit pulping or juice extraction, leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, macroporous resin separation or Other extraction methods.
  • the composition is in the form of fresh fruit, juice, jam, fruit powder (such as freeze-dried powder), candy (such as tablet candy), oral liquid, capsule, tablet, dried fruit, preserved fruit, fruit wine or pastry.
  • the product form of the composition may be fresh fruit, juice, jam, fruit powder (such as freeze-dried powder), candy (such as tablet candy), oral liquid, capsule, tablet, dried fruit, preserved fruit, fruit wine or Pastries etc. That is to say, the composition can be made into fresh fruit, juice, jam, fruit powder (such as freeze-dried powder), candy (such as tablet candy), oral liquid, capsule, tablet, dried fruit, preserved fruit, fruit wine or pastry, etc. .
  • juice, jam, fruit powder such as freeze-dried powder
  • candy such as tablet candy
  • oral liquid, capsule, and tablet can be extracted by leaching, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, macroporous resin separation or other methods.
  • the extract obtained by extraction method is prepared.
  • the composition is in the form of medicine (such as traditional Chinese medicine, Chinese patent medicine, natural medicine, etc.), ordinary food or functional food or health food.
  • the composition can be made into medicine (such as traditional Chinese medicine, Chinese patent medicine, natural medicine, etc.), ordinary food or functional food or health food.
  • the red-fleshed apple refers to a fruit of a plant of the genus Malus of the Rosaceae family with red flesh.
  • the red-fleshed apple is selected from: Hongxun No. 1 (Hongxun No. 1), Hongxun No. 3 (Hongxun No. 3), Hongxun No. 4 (Hongxun No. 4), Hongxun No. 6 No. (Hongxun No. 6), Hongxun No. 8 (Hongxun No. 8), Red Love, etc.
  • the cherry plum is selected from the group consisting of cherry plum red fruit, cherry plum black fruit, cherry plum purple fruit, cherry plum purple black fruit, cherry plum yellow fruit, and the like.
  • the invention provides a combination product comprising:
  • a first product which is the aforementioned composition
  • the second product is a drug for preventing and/or treating atherosclerotic disease.
  • the atherosclerotic disease is selected from the group consisting of atherosclerosis of the aorta and its major branches, coronary atherosclerosis, cerebral atherosclerosis, renal atherosclerosis, mesenteric atherosclerosis, limb atherosclerosis Atherosclerosis, etc.
  • the drug for preventing and/or treating atherosclerotic disease is selected from statins (such as rosuvastatin, atorvastatin, simvastatin), ezetimibe, PCSK9 inhibitors , aspirin, clopidogrel, colchicine, canakinumab, probucol, idebenone, etc.
  • statins such as rosuvastatin, atorvastatin, simvastatin
  • PCSK9 inhibitors aspirin, clopidogrel, colchicine, canakinumab, probucol, idebenone, etc.
  • the weight ratio of the first product and the second product is 1000:0.001-1000:33.4 (such as 1000:0.002075, 1000:0.004175, 1000:0.0083, 1000:0.02075, 1000:0.04175 , 1000:0.083, 1000:0.08325, 1000:0.167, 1000:0.20875, 1000:0.3125, 1000:0.333, 1000:0.4175, 1000:0.62625, 1000:0.835, 1000:1.25, 100 0:1.67, 1000:2.0875, 1000 :2.505, 1000:2.875, 1000:4.175, 1000:8.35 or 1000:16.7, or 1000:0.002075-1000:16.7).
  • the weight ratio of the first product to the second product is 1000:0.01-1000:0.34 (such as 1000:0.02075, 1000:0.04175 , 1000:0.083 or 1000:0.167, or such as 1000:0.02075-1000:0.167).
  • the weight ratio of the first product to the second product is 1000:0.02-1000:0.67 (such as 1000:0.04175, 1000:0.08325 , 1000:0.167 or 1000:0.333, or such as 1000:0.04175-1000:0.333).
  • the weight ratio of the first product to the second product is 1000:0.10-1000:5.01 (such as 1000:0.20875, 1000:0.4175, 1000: 0.62625, 1000:0.835, 1000:1.67 or 1000:2.505, or 1000:0.20875-1000:2.505).
  • the weight ratio of the first product to the second product is 1000:0.10-1000:5.01 (such as 1000:0.20875, 1000:0.4175, 1000:0.62625, 1000:0.835, 1000:1.67 or 1000:2.505, or 1000:0.20875-1000:2.505).
  • the weight ratio of the first product to the second product is 1000:0.001-1000:0.166 (such as 1000:0.002075, 1000:0.004175, 1000:0.0083, 1000:0.02075 or 1000:0.083, or such as 1000:0.002075-1000:0.083).
  • the weight ratio of the first product and the second product is 1000:1.0-1000:33.4 (such as 1000:2.0875, 1000:2.875, 1000:4.175, 1000:8.35 or 1000:16.7, or 1000:2.0875-1000:16.7).
  • the present invention provides the use of the aforementioned composition in the preparation of drugs (such as traditional Chinese medicine, Chinese patent medicine, natural medicine, etc.), ordinary food or functional food or health food, and the drug (such as traditional Chinese medicine, traditional Chinese medicine, natural medicine, etc.) Chinese patent medicines, natural medicines, etc.), ordinary foods or functional foods or health foods are used to prevent and/or treat atherosclerotic diseases.
  • drugs such as traditional Chinese medicine, Chinese patent medicine, natural medicine, etc.
  • the drug such as traditional Chinese medicine, traditional Chinese medicine, natural medicine, etc.
  • Chinese patent medicines, natural medicines, etc. Chinese patent medicines, natural medicines, etc.
  • ordinary foods or functional foods or health foods are used to prevent and/or treat atherosclerotic diseases.
  • ordinary food refers to finished products and raw materials for human consumption or drinking, as well as items that are both food and medicine according to tradition, but does not include items for therapeutic purposes.
  • health food refers to food that claims to have specific health functions or is for the purpose of supplementing vitamins and minerals, that is, it is suitable for consumption by specific groups of people, has the function of regulating the body, is not for the purpose of treating diseases, and does not produce any harmful effects on the human body. Any food that poses an acute, subacute or chronic hazard. Functional food is mainly a term used in Europe, America and Japan for foods that can improve physical health or reduce disease, similar to the domestic definition of health food.
  • traditional Chinese medicine refers to medicines whose collection, processing, and preparation are guided by traditional Chinese medicine theory, whose mechanism of action is explained, and whose clinical application is guided.
  • Traditional Chinese medicine mainly comes from natural medicines and their processed products, including botanical medicines, animal medicines, mineral medicines and some chemical and biological medicines, with the majority of them being botanical medicines.
  • Chinese patent medicines are made of Chinese medicinal materials as raw materials. Under the guidance of Chinese medicine theory, in order to prevent and treat diseases, they are processed into Chinese medicine products of a certain dosage form according to prescribed prescriptions and preparation techniques. They are approved by the National Drug Administration. Commercialized Class I traditional Chinese medicine preparations approved by the regulatory authorities.
  • natural drugs refer to animal drugs, botanical drugs, mineral drugs, etc. that have been proven to have certain pharmacological activity by the modern medical system.
  • Natural medicine is not the same as traditional Chinese medicine or herbal medicine. Natural medicine refers to natural medicinal substances and their preparations used under the guidance of modern medical theory. Its sources include plants, animals and minerals, and generally do not include substances derived from genetically modified animals and plants, or substances that have been fermented by microorganisms or modified chemically.
  • the first product is a red-fleshed apple and cherry plum combination product
  • the second product is an anti-atherosclerotic drug (such as hypolipidemic, antiplatelet) , anticoagulant drugs, anti-inflammatory and anti-oxidative stress drugs, etc.).
  • “individual” includes humans or non-human animals.
  • Exemplary human subjects include human subjects (referred to as patients) suffering from a disease, such as those described herein, or normal subjects.
  • “Non-human animals” as used herein include all vertebrates, such as non-mammals (e.g. fish, birds, amphibians, reptiles) and mammals, such as non-human primates, domestic animals and/or domesticated animals (e.g. Rat, sheep, dog, cat, cow, pig, etc.).
  • cherry plum extract or “red-fleshed apple extract” refers to using cherry plums or red-fleshed apples as raw materials to obtain and enrich cherry plums or red-fleshed apples through a physical and/or chemical extraction and separation process.
  • a product formed from one or more active ingredients in food which can be used as a raw material or excipient for food (such as general food or functional food or health food) or medicine.
  • Exemplary extraction methods include, but are not limited to: leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, macroporous resin separation, etc.
  • the red-fleshed apple and cherry plum extracts of the present invention are obtained by extraction (eg, water extraction or alcohol extraction).
  • the leach extraction includes the following steps:
  • Step 1 Remove seeds or cores from red-fleshed apples and cherry plums respectively and then pulp to directly obtain pulp, or separate juice and pomace; in some embodiments, use gauze (such as 100-300 mesh gauze, such as 200 mesh gauze) filter to separate the juice and pomace;
  • gauze such as 100-300 mesh gauze, such as 200 mesh gauze
  • Step 2 Mix the pulp or pomace with the extraction medium (such as water or ethanol), and add cellulase to extract at normal temperature or heating (such as 10-100°C, such as 50°C) (such as extraction for 30-100 minutes) , for example 60 min), filter to obtain the pulp or pomace extract; in some embodiments, the pulp or pomace and the extraction medium are mixed at a weight ratio of 1: (2-10) (for example, 1:5); in In certain embodiments, the ethanol is 20%-100% (such as 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% or 100%) ethanol;
  • the extraction medium such as water or ethanol
  • Step 3 Concentrate the pulp extract or the combined solution of fruit juice and pomace extract to obtain the extract of red-fleshed apple and cherry plum; in some embodiments, concentration is performed by rotary evaporation; in some embodiments, the extract is concentrated by rotary evaporation. In an embodiment, the temperature of rotary evaporation is 30-60°C, such as 50°C;
  • Step 4 Mix the extracted red-fleshed apple and cherry plum extracts in different proportions (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1: 4. 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1)
  • Weight ratio (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1: 4. 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1
  • Prepare a composition or first mix red-fleshed apple and cherry plum raw materials in different proportions as mentioned above in step 1, and then extract.
  • the red-fleshed apple and cherry plum extracts of the present invention are obtained by water extraction.
  • the water extraction includes the following steps:
  • Step 1 Remove the seeds or cores of red-fleshed apples and cherry plums respectively and then beat them to obtain pulp directly, or filter the juice with 100-300 mesh gauze to obtain red-fleshed apple and cherry plum juice, and separate the juice and pomace;
  • the mesh number of the gauze is 200 mesh;
  • Step 2 Mix red-fleshed apple and cherry plum pulp or pomace with water according to the material-to-liquid ratio of 1: (2-10), add cellulase, extract at 10-100°C for 30-100 minutes, and filter to obtain the fruit.
  • Pulp or pomace extract in some embodiments, the material-to-liquid ratio is 1:5; in some embodiments, the extraction temperature is 50°C; in some embodiments, the extraction time is 60 minutes ; In certain embodiments, the amount of cellulase added (based on the weight of the pomace or pulp) is 0.2%-1% (for example, 0.5%);
  • Step 3 Red-fleshed apple and cherry plum pulp extract or the combined solution of juice and pomace extract is concentrated by rotary evaporation at 30-60°C to prepare red-fleshed apple and cherry plum aqueous extract; in some cases In the implementation, the rotary evaporation temperature is selected 50°C;
  • Step 4 Mix the extracted red-fleshed apple and cherry plum water extracts in different proportions (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5 ,1:4,1:3,1:2,1:1,2:1,3:1,4:1,5:1,6:1,7:1,8:1,9:1,10 :1) Prepare a composition, or first mix red-fleshed apple and cherry plum raw materials in different proportions as mentioned above in step 1, and then extract.
  • the red-fleshed apple and cherry plum extracts of the present invention are obtained by alcohol extraction.
  • the alcohol extraction includes the following steps:
  • Step 1 Remove the seeds or cores of red-fleshed apples and cherry plums respectively and then beat them to obtain pulp directly, or filter the juice with 100-300 mesh gauze to obtain red-fleshed apple and cherry plum juice, and separate the juice and pomace;
  • the mesh number of the gauze is 200 mesh;
  • Step 2 Red-fleshed apple and cherry plum pulp or pomace according to the material-liquid ratio 1: (2-10) and 20%-100% (such as 20%, 30%, 40%, 50%, 60%, 70% , 80%, 90% or 100%) ethanol mixture, add 0.2%-1% (based on the weight of the pomace or pulp) (such as 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8% , 0.9% or 1%) cellulase, extract at 10-100°C for 30-100 minutes, and filter to obtain pulp or pomace extract; in some embodiments, the material-to-liquid ratio is selected to be 1:5 ; In some embodiments, the concentration of ethanol used is 40%; in some embodiments, the extraction temperature is 50°C; in some embodiments, the extraction time is 60 minutes;
  • Step 3 The red-fleshed apple and cherry plum pulp extract or the combined solution of juice and pomace extract is concentrated by rotary evaporation at 30-60°C.
  • the pulp extract is directly concentrated to obtain the red-fleshed apple and cherry plum alcohol infusion. Extract the extract; in some embodiments, the rotary evaporation temperature is selected to be 50°C; optionally, the extract is evaporated to remove residual ethanol;
  • Step 4 Mix the extracted red-fleshed apple and cherry plum alcohol extracts in different proportions (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5 ,1:4,1:3,1:2,1:1,2:1,3:1,4:1,5:1,6:1,7:1,8:1,9:1,10 :1) Prepare a composition, or first mix red-fleshed apple and cherry plum raw materials in different proportions as mentioned above in step 1, and then extract.
  • the red-fleshed apple and cherry plum extracts of the present invention are obtained by ultrasonic extraction (eg, ultrasonic water extraction or ultrasonic alcohol extraction).
  • the ultrasonic extraction includes the following steps:
  • Step 1 Remove seeds or cores from red-fleshed apples and cherry plums respectively and then pulp to directly obtain pulp, or separate juice and pomace; in some embodiments, use gauze (such as 100-300 mesh gauze, such as 200 mesh gauze) filter to separate the juice and pomace;
  • gauze such as 100-300 mesh gauze, such as 200 mesh gauze
  • Step 2 Mix the pulp or pomace with extraction medium (such as water or ethanol) and cellulase, and extract under heating conditions (such as 30-50°C, such as 45°C) (such as extraction for 30-100min, such as 60min) , then ultrasonicate (for example, ultrasonic for 20-60min, for example, ultrasonic for 30min) under ultrasonic conditions (for example, ultrasonic power of 100-500W), and filter to obtain pulp or pomace extract; in some embodiments, the addition of cellulase
  • the amount (based on the weight of the pomace or pulp) is 0.2%-1% (for example, 0.5%); in some embodiments, the pulp or pomace and the extraction medium are in a ratio of 1: (2-10) (for example, 1 :5) is mixed with a weight ratio of 100%) ethanol;
  • Step 3 Concentrate the red-fleshed apple and cherry plum pulp extract or the combined solution of juice and pomace extract to obtain the ultrasonic extract of red-fleshed apple and cherry plum; in some embodiments, by rotary evaporation Concentration; in certain embodiments, the temperature of rotary evaporation is 30-60°C, such as 50°C;
  • Step 4 Mix the extracted red-fleshed apple and cherry plum ultrasonic extracts in different proportions (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1 :4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1 ) is prepared into a composition, or in step 1, first mix red-fleshed apple and cherry plum raw materials in different proportions as mentioned above, and then extract.
  • the red-fleshed apple and cherry plum extracts of the present invention are obtained by ultrasonic water extraction.
  • the ultrasonic water extraction includes the following steps:
  • Step 1 Remove the seeds or cores of red-fleshed apples and cherry plums respectively and then beat them to obtain pulp directly, or filter the juice with 100-300 mesh gauze to obtain red-fleshed apple and cherry plum juice, and separate the juice and pomace;
  • the mesh number of the gauze is 200 mesh;
  • Step 2 Mix the red-fleshed apple and cherry plum pulp or pomace with water according to the material-to-liquid ratio 1: (2-10), and add 0.2%-1% (based on the weight of the pomace or pulp) of cellulose.
  • Enzyme extract in 30-50°C water bath for 30-100 minutes, 100-500W ultrasonic for 20-60 minutes, filter to obtain pulp or pomace extract; in some embodiments, the material-liquid ratio is selected to be 1:5; in some embodiments In the scheme, the addition amount of cellulase is 0.5%; in some embodiments, the water bath temperature is 45°C; in some embodiments, the extraction time is 60 minutes; in some embodiments, the ultrasonic power is 500W; in In some embodiments, the ultrasonic time is 30 minutes;
  • Step 3 The combined solution of red-fleshed apple and cherry plum pulp or fruit juice and pomace extract is concentrated by rotary evaporation at 30-60°C to prepare red-fleshed apple and cherry plum ultrasonic extraction aqueous extract; in certain embodiments , the rotary evaporation temperature is selected as 50°C;
  • Step 4 Mix the extracted red-fleshed apple and cherry plum ultrasonic aqueous extracts according to different proportions (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10: 1) Prepare a composition, or first mix red-fleshed apples and cherry plums in different proportions in step 1 material, and then extract.
  • the red-fleshed apple and cherry plum extracts of the present invention are obtained by ultrasonic alcohol extraction.
  • the ultrasonic alcohol extraction includes the following steps:
  • Step 1 Remove the seeds or cores of red-fleshed apples and cherry plums respectively and then beat them to obtain pulp directly, or filter the juice with 100-300 mesh gauze to obtain red-fleshed apple and cherry plum juice, and separate the juice and pomace;
  • the mesh number of the gauze is 200 mesh;
  • Step 2 Mix the red-fleshed apple and cherry plum pulp or pomace with 20%-100% ethanol according to the material-to-liquid ratio 1: (2-10), and add 0.2%-1% (based on the weight of the pomace or pulp (based on) cellulase, extract in 30-50°C water bath for 30-100min, 100-500W ultrasonic for 20-60min, filter to obtain pulp or pomace extract; in some embodiments, the material-to-liquid ratio is selected as 1: 5; In some embodiments, the concentration of ethanol used is 40%; in some embodiments, the cellulase addition amount is 0.5%; in some embodiments, the water bath temperature is 45°C; in a certain In some embodiments, the extraction time is 60 minutes; in some embodiments, the ultrasonic power is 500W; in some embodiments, the ultrasonic time is 30 minutes;
  • Step 3 Red-fleshed apple and cherry plum pulp extract or the combined solution of juice and pomace extract is concentrated by rotary evaporation at 30-60°C to prepare red-fleshed apple and cherry plum ultrasonic alcohol extract; in some cases
  • the rotary evaporation temperature is selected to be 50°C; optionally, the extract is evaporated to remove residual ethanol;
  • Step 4 Mix the extracted red-fleshed apple and cherry plum ultrasonic alcohol extracts in different proportions (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10: 1) Prepare a composition, or in step 1, first mix red-fleshed apple and cherry plum raw materials in different proportions as mentioned above, and then extract.
  • the red-fleshed apple and cherry plum extracts of the present invention are obtained by ultra-high pressure extraction (eg, ultra-high pressure water extraction or ultra-high pressure alcohol extraction).
  • ultra-high pressure refers to pressures exceeding 100 MPa (equivalent to 1,000 atmospheres).
  • the ultrahigh pressure extraction includes the following steps:
  • Step 1 Remove seeds or cores from red-fleshed apples and cherry plums respectively and then pulp to directly obtain pulp, or separate juice and pomace; in some embodiments, use gauze (such as 100-300 mesh gauze, such as 200 mesh gauze) filter to separate the juice and pomace;
  • gauze such as 100-300 mesh gauze, such as 200 mesh gauze
  • Step 2 Mix the pulp or pomace with the extraction medium (such as water or ethanol), process it under ultra-high pressure (such as 200-600MPa, such as 400MPa) conditions (such as 3-10min, such as 5min), and filter to obtain the fruit.
  • Pulp or pomace extract in certain embodiments, the pulp or pomace is mixed with the extraction medium at a weight ratio of 1: (2-10) (for example, 1:5);
  • the ethanol is 50%-95% ethanol;
  • Step 3 Concentrate the combined liquid of red-fleshed apple and cherry plum pulp or juice and pomace extract to obtain ultra-high pressure extract of red-fleshed apple and cherry plum; in some embodiments, concentration is performed by rotary evaporation ; In certain embodiments, the temperature of rotary evaporation is 30-60°C, such as 50°C;
  • Step 4 Mix the extracted red-fleshed apple and cherry plum ultra-high pressure extracts in different proportions (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10: 1) Prepare a composition, or in step 1, first mix red-fleshed apple and cherry plum raw materials in different proportions as mentioned above, and then extract.
  • the red-fleshed apple and cherry plum extracts of the present invention are obtained by ultrahigh-pressure water extraction.
  • the ultra-high-pressure water extraction includes the following steps:
  • Step 1 Remove the seeds or cores of red-fleshed apples and cherry plums respectively and then beat them to obtain pulp directly, or filter the juice with 100-300 mesh gauze to obtain red-fleshed apple and cherry plum juice, and separate the juice and pomace;
  • the mesh number of the gauze is 200 mesh;
  • Step 2 Red-fleshed apple and cherry plum pulp or pomace are mixed with water according to the material-to-liquid ratio of 1: (2-10), treated with ultra-high pressure 200-600MPa for 3-10 minutes, and filtered to obtain pulp or pomace extract;
  • the material-to-liquid ratio is 1:5; in some embodiments, the ultra-high pressure pressure is 400MPa; in some embodiments, the ultra-high pressure treatment time is 5 minutes;
  • Step 3 Red-fleshed apple and cherry plum pulp extract or the combined solution of juice and pomace extract is concentrated by rotary evaporation at 30-60°C to prepare red-fleshed apple and cherry plum ultra-high pressure water extract;
  • the rotary evaporation temperature is selected to be 50°C;
  • Step 4 Mix the extracted red-fleshed apple and cherry plum ultra-high pressure water extracts in different proportions (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5 ,1:4,1:3,1:2,1:1,2:1,3:1,4:1,5:1,6:1,7:1,8:1,9:1,10 :1) Prepare a composition, or first mix red-fleshed apple and cherry plum raw materials in different proportions as mentioned above in step 1, and then extract.
  • the red-fleshed apple and cherry plum extracts of the present invention are obtained by ultrahigh-pressure alcohol extraction.
  • the ultra-high-pressure alcohol extraction includes the following steps:
  • Step 1 Remove the seeds or cores of red-fleshed apples and cherry plums respectively and then beat them to obtain pulp directly, or filter the juice with 100-300 mesh gauze to obtain red-fleshed apple and cherry plum juice, and separate the juice and pomace;
  • the mesh number of the gauze is 200 mesh;
  • Step 2 Red-fleshed apple and cherry plum pulp or pomace according to the material-to-liquid ratio 1: (2-10) and 20%-100% (such as 20%, 30%, 40%, 50%, 60%, 70% , 80%, 90% or 100%) ethanol mixture, ultra-high pressure 200-600MPa Process for 3-10 minutes, and filter to obtain pulp or pomace extract;
  • the material-to-liquid ratio is 1:5;
  • the ultra-high pressure is 400MPa; in some embodiments , the ultrahigh pressure treatment time is selected to be 5 minutes; in some embodiments, the ethanol is 40% ethanol;
  • Step 3 Red-fleshed apple and cherry plum pulp extract or the combined solution of juice and pomace extract is concentrated by rotary evaporation at 30-60°C to obtain red-fleshed apple and cherry plum ultra-high pressure alcohol extract;
  • the rotary evaporation temperature is selected to be 50°C; optionally, the extract is evaporated to remove residual ethanol;
  • Step 4 Mix the extracted red-fleshed apple and cherry plum ultra-high pressure alcohol extracts in different proportions (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5 ,1:4,1:3,1:2,1:1,2:1,3:1,4:1,5:1,6:1,7:1,8:1,9:1,10 :1) Prepare a composition, or first mix red-fleshed apple and cherry plum raw materials in different proportions as mentioned above in step 1, and then extract.
  • Subcritical extraction also known as “subcritical extraction”.
  • red-fleshed apple and cherry plum extracts are obtained by subcritical extraction (eg, subcritical water extraction or subcritical alcohol extraction).
  • the subcritical extraction includes the following steps:
  • Step 1 Remove seeds or cores from red-fleshed apples and cherry plums respectively and then pulp to directly obtain pulp, or separate juice and pomace; in some embodiments, use gauze (such as 100-300 mesh gauze, such as 200 mesh gauze) filter to separate the juice and pomace;
  • gauze such as 100-300 mesh gauze, such as 200 mesh gauze
  • Step 2 Mix the pulp or pomace with an extraction medium (such as water or ethanol), perform subcritical extraction treatment (such as treatment for 10-50 minutes, such as 30 minutes), and filter to obtain the pulp or pomace extract; in some embodiments
  • the pulp or pomace is mixed with the extraction medium at a weight ratio of 1: (2-10) (for example, 1:5); in certain embodiments, the ethanol is 50%-95% ethanol; in a certain
  • the processing temperature of subcritical extraction is 100-150°C (for example, 110°C);
  • Step 3 Concentrate the combined liquid of pulp or juice and pomace extract to obtain subcritical extract of red-fleshed apple and cherry plum; in some embodiments, concentration is performed by rotary evaporation; in some embodiments , the temperature of rotary evaporation is 30-60°C, such as 50°C;
  • Step 4 Mix the extracted red-fleshed apple and cherry plum subcritical extracts in different proportions (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10: 1) Prepare a composition, or in step 1, first mix red-fleshed apple and cherry plum raw materials in different proportions as mentioned above, and then extract.
  • the red-fleshed apple and cherry plum extracts of the present invention are obtained by subcritical water extraction.
  • the subcritical water extraction includes the following steps:
  • Step 1 Remove the seeds or cores of red-fleshed apples and cherry plums respectively and then beat them to obtain pulp directly, or filter the juice with 100-300 mesh gauze to obtain red-fleshed apple and cherry plum juice, and separate the juice and pomace;
  • the mesh number of the gauze is 200 mesh;
  • Step 2 Red-fleshed apple and cherry plum pulp or pomace are mixed with water according to the material-to-liquid ratio of 1: (2-10), subcritical extraction is performed for 10-50 minutes, the processing temperature is 100-150°C, and the pulp or pomace is obtained by filtration.
  • Pomace extract liquid in some embodiments, the material-to-liquid ratio is 1:5; in some embodiments, the treatment temperature is 110°C; in some embodiments, the treatment time is 30 minutes;
  • Step 3 Red-fleshed apple and cherry plum pulp extract or the combined solution of juice and pomace extract is concentrated by rotary evaporation at 30-60°C to obtain red-fleshed apple and cherry plum subcritical water extract;
  • the rotary evaporation temperature is selected to be 50°C;
  • Step 4 Mix the extracted red-fleshed apple and cherry plum subcritical water extracts in different proportions (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5 ,1:4,1:3,1:2,1:1,2:1,3:1,4:1,5:1,6:1,7:1,8:1,9:1,10 :1) Prepare a composition, or first mix red-fleshed apple and cherry plum raw materials in different proportions as mentioned above in step 1, and then extract.
  • the red-fleshed apple and cherry plum extracts of the present invention are obtained by subcritical alcohol extraction.
  • the subcritical alcohol extraction includes the following steps:
  • Step 1 Remove the seeds or cores of red-fleshed apples and cherry plums respectively and then beat them to obtain pulp directly, or filter the juice with 100-300 mesh gauze to obtain red-fleshed apple and cherry plum juice, and separate the juice and pomace;
  • the mesh number of the gauze is 200 mesh;
  • Step 2 Red-fleshed apple and cherry plum pulp or pomace according to the material-to-liquid ratio 1: (2-10) and 20%-100% (such as 20%, 30%, 40%, 50%, 60%, 70% , 80%, 90% or 100%) ethanol, subcritical extraction for 10-50 min, the processing temperature is 100-150°C, and filtered to obtain the pulp or pomace extract; in some embodiments, the material-to-liquid ratio Select 1:5; in some embodiments, the treatment temperature is 110°C; in some embodiments, the treatment time is 30 minutes;
  • Step 3 Red-fleshed apple and cherry plum pulp extract or a combined solution of juice and pomace extract is concentrated by rotary evaporation at 30-60°C to obtain red-fleshed apple and cherry plum subcritical alcohol extract;
  • the rotary evaporation temperature is selected to be 50°C; optionally, the extract is evaporated to remove residual ethanol;
  • Step 4 Mix the extracted red-fleshed apple and cherry plum subcritical alcohol extracts in different proportions (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5 ,1:4,1:3,1:2,1:1,2:1,3:1,4:1,5:1,6:1,7:1,8:1,9:1,10 :1) Prepare a composition, or first mix red-fleshed apple and cherry plum raw materials in different proportions as mentioned above in step 1, and then extract.
  • red-fleshed apple and cherry plum extracts are isolated by macroporous resin.
  • the macroporous resin separation includes the following steps:
  • Step 1 Prepare red-fleshed apple and cherry plum extracts by the above-mentioned different extraction methods (leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, etc.); or red-fleshed apples and cherry plums can be directly pulped to obtain the fruit. Pulp;
  • Step 2 Mix the extract or pulp of the above red-fleshed apples and cherry plums with 95%-100% ethanol.
  • the mixing ratio of the extract or pulp to ethanol is 1: (2-10) (weight ratio) (for example 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9 or 1:10); mixing time is 1-5 hours (e.g. 3 hours), Afterwards, filtration and separation are performed to obtain the alcoholic juice and precipitation.
  • the ethanol is removed from the alcoholic juice by rotary evaporation.
  • the temperature of the rotary evaporation is 30-60°C (such as 30°C, 40°C, 50°C or 60°C), and the clear alcoholic juice is obtained;
  • the extracts of the above-mentioned red-fleshed apples and cherry plum extracts or the pulps of red-fleshed apples and cherry plums can also be added to pectinase for enzymatic hydrolysis and clarification treatment to obtain enzymatic clear juice
  • the enzymatic hydrolysis temperature is 20 -60°C (such as 20°C, 30°C, 40°C, 50°C or 60°C)
  • enzymatic hydrolysis time is 30-240min (such as 30min, 60min, 90min, 120min, 150min, 180min, 210min or 240min), pectinase
  • the addition amount is 100-1000ppm (such as 100ppm, 200ppm, 300ppm, 400ppm, 500ppm, 600ppm, 700ppm, 800ppm, 900ppm or 1000
  • Step 3 Use macroporous adsorption resin to adsorb the above-mentioned alcohol precipitation clear juice or enzymatic hydrolysis clear juice, and sequentially use water and 20%-100% ethanol (such as 20% ethanol, 40% ethanol, 60% ethanol, 80% ethanol, 100% ethanol, etc. % ethanol) perform gradient elution, and collect the eluate of each gradient separately; in some embodiments, the elution speed is 1 to 5 BV/h (1 to 5 column volumes);
  • Step 4 Rotate the above eluent to remove ethanol.
  • the rotary evaporation temperature is 30-60°C (such as 30°C, 40°C, 50°C or 60°C) to obtain separation samples of different macroporous resins.
  • Red-fleshed apples and cherry plums can be extracted and prepared separately and then mixed in proportion to prepare a composition.
  • red-fleshed apple and cherry plum raw materials can be mixed in different proportions and then extracted to prepare the composition.
  • the product form of the composition of the present invention can be fresh fruit, juice, jam, fruit powder (such as freeze-dried powder), candy (such as tablet candy), oral liquid, capsule, tablet, dried fruit, preserved fruit, Cider or pastries.
  • juice, jam, fruit powder (such as freeze-dried powder), candy (such as tablet candy), oral liquid, capsule, and tablet can be extracted by leaching, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, macroporous resin separation or other methods. The extract obtained by extraction method is prepared.
  • the product form of the composition of the present invention may be medicine, general food or functional food or health food.
  • product forms such as juice, jam, fruit powder (such as freeze-dried powder), candy (such as tablet candy), oral liquid, capsule, tablet, dried fruit, preserved fruit, fruit wine or pastry
  • the composition can be used as the main raw material, for example, accounting for more than 30wt%, more than 40wt%, more than 50wt%, more than 60wt%, more than 70wt%, more than 80wt%, more than 90wt% or 100wt% of the total raw materials; optionally, the Product is OK To include other ingredients such as water, sweetening substances, or other types of fruits or their extracts.
  • red-fleshed apple and cherry plum products eg, fresh fruit, juice, jam, fruit powder, tableted candy, oral liquid, capsule.
  • red-fleshed apples and cherry plums can be obtained by a method comprising the following steps:
  • Step 1 Harvest red-fleshed apples and cherry plums from 1 month before maturity to when they are fully mature. When picking fruits, give priority to picking fruits on sunny days;
  • Step 2 Select and remove rotten fruits or fruits with poor color
  • Step 3 Refrigerate and store after harvest
  • Step 4 Combine red-fleshed apples and cherry plums in different proportions (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1: 3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1).
  • the obtained red-fleshed apple and cherry plum fresh fruits can be eaten directly.
  • red flesh apple and cherry plum juice can be obtained by a method comprising the following steps:
  • Step 1 Seed or core the red-fleshed apples and cherry plums respectively and then beat;
  • Step 2 Filter the slurry (for example, filter it through 100-300 mesh gauze) to obtain red-fleshed apple and cherry plum juice cloudy juice; in some embodiments, the mesh number of the gauze is 200 mesh;
  • Step 3 Mix red apple and cherry plum juice in different proportions (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1 :3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) prepared into a combination material, or in step 1, first mix red-fleshed apple and cherry plum raw materials in different proportions as mentioned above, and then beat to prepare juice.
  • weight ratio 1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1 :3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1
  • red-fleshed apple and cherry plum juices can also be obtained by a method comprising the following steps:
  • Step 1 Seed or core the red-fleshed apples and cherry plums respectively and then beat;
  • Step 2 Filter the slurry (for example, filter it through 100-300 mesh gauze), add pectinase for enzymatic hydrolysis and filtration; prepare red-fleshed apple and cherry plum juice clear juice; in some embodiments, the purpose of the gauze is The number is 200 mesh;
  • Step 3 Mix red apple and cherry plum juice in different proportions (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1: 3. 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) prepared into a composition , or in step 1, first mix red-fleshed apple and cherry plum raw materials in different proportions as mentioned above, and then beat to prepare juice.
  • red-fleshed apple and cherry plum juices can also be prepared from extracts obtained by the aforementioned leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, macroporous resin separation or other extraction methods as raw materials.
  • red flesh apple and cherry plum jam can be obtained by a method comprising the following steps:
  • Step 1 Seed or core the red-fleshed apples and cherry plums respectively and then beat;
  • Step 2 Boil the pulp, heat and concentrate to make red-fleshed apple and cherry plum jam
  • Step 3 Mix red flesh apple and cherry plum jam according to different proportions (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1: 3. 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) prepared into a composition , or in step 1, first mix red-fleshed apple and cherry plum raw materials in different proportions as mentioned above, and then beat to prepare jam.
  • weight ratio weight ratio
  • red-fleshed apple and cherry plum jams can also be prepared from extracts or juices obtained by the aforementioned leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, macroporous resin separation or other extraction methods as raw materials. .
  • red-fleshed apple and cherry plum fruit powder can be obtained by a method comprising the following steps:
  • Step 1 Remove seeds or cores from red-fleshed apples and cherry plums and then beat them into pulp.
  • Step 2 freeze-dry the pulp to make pulp freeze-dried powder
  • Step 3 Mix red flesh apple and cherry plum freeze-dried powder according to different proportions (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) prepared composition, or in step 1, first mix red-fleshed apple and cherry plum raw materials in different proportions as mentioned above, and then beat to prepare freeze-dried powder.
  • red-fleshed apple and cherry plum fruit powder can also be obtained by a method comprising the following steps:
  • Step 1 Seed or core the red-fleshed apples and cherry plums respectively and then beat;
  • Step 2 Filter the slurry (for example, filter it through 100-300 mesh gauze) to prepare red-fleshed apple and cherry plum juice; in some embodiments, the mesh number of the gauze is 200 mesh;
  • Step 3 freeze-dry the juice to make juice freeze-dried powder
  • Step 4 Mix red flesh apple and cherry plum freeze-dried powder according to different proportions (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) prepared composition, or in step 1, first mix red-fleshed apple and cherry plum raw materials in different proportions as mentioned above, and then beat to prepare freeze-dried powder.
  • red-fleshed apple and cherry plum fruit powder can also be prepared using belt drying by referring to the above steps.
  • red-fleshed apple and cherry plum fruit powder can also be prepared from the extract obtained by the aforementioned leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, macroporous resin separation or other extraction methods as raw materials, by Freeze drying or belt drying is used to prepare fruit powder.
  • red-fleshed apple and cherry plum pressed candy can be obtained by a method comprising the following steps:
  • Step 1 Seed or core the red-fleshed apples and cherry plums respectively and then beat;
  • Step 2 freeze-dry or belt-dry the pulp to make powder
  • Step 3 Compress the fruit powder and auxiliary materials into tablets to make tablet candy
  • Step 4 Mix the red-fleshed apple and cherry plum tablet candies in different proportions (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) prepared composition, or in step 1, first mix red-fleshed apple and cherry plum raw materials in different proportions as mentioned above, and then beat to prepare tableted candy.
  • weight ratio 1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1
  • red-fleshed apple and cherry plum pressed candy can also be obtained by a method comprising the following steps:
  • Step 1 Seed or core the red-fleshed apples and cherry plums respectively and then beat;
  • Step 2 Filter the slurry (for example, filter it through 100-300 mesh gauze) to prepare red-fleshed apple juice; in some embodiments, the mesh number of the gauze is 200 mesh;
  • Step 3 freeze-dry or belt-dry the juice into powder
  • Step 4 Compress the fruit powder and auxiliary materials into tablets to make tablet candy.
  • Step 5 Mix the red-fleshed apple and cherry plum tablet candies in different proportions (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) prepared composition, or in step 1, first mix red-fleshed apple and cherry plum raw materials in different proportions as mentioned above, and then beat to prepare tableted candy.
  • weight ratio 1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1
  • red-fleshed apple and cherry plum tablet candies can also be prepared from extracts obtained by the aforementioned leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, macroporous resin separation or other extraction methods as raw materials. .
  • red-fleshed apple and cherry plum oral liquids can be obtained by a method comprising the following steps:
  • Step 1 Prepare different ratios (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1 , 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) of red-fleshed apple and cherry plum extracts (e.g., by Produced by fresh fruit pulping or juice extraction, leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction or macroporous resin separation, the steps are the same as above);
  • Step 2 Sterilize the red-fleshed apple and cherry plum extracts to prepare the red-fleshed apple oral liquid.
  • red-fleshed apple and cherry plum oral liquids can also be obtained by a method comprising the following steps:
  • Step 1 Prepare different ratios (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) of red-fleshed apple and cherry plum extracts (for example, it can be obtained by fresh fruit pulping or juice extraction, leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction or macroporous resin separation, the steps are the same as above);
  • Step 2 Add pectinase for enzymatic hydrolysis and filtration to prepare a clear juice extract composition
  • Step 3 Sterilize the red-fleshed apple and cherry plum juice extracts to prepare the red-fleshed apple oral liquid.
  • red-fleshed apple and cherry plum capsules can be obtained by a method comprising the following steps:
  • Step 1 Seed or core the red-fleshed apples and cherry plums respectively and then beat;
  • Step 2 freeze-dry the pulp or belt dry it to make pulp freeze-dried powder
  • Step 3 Mix red-fleshed apple and cherry plum fruit powder in different proportions (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1: 3. 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) prepared into a composition , or in step 1, first mix red-fleshed apple and cherry plum raw materials in different proportions as mentioned above, and then beat to prepare fruit powder;
  • Step 4 Put the fruit powder into the capsule shell to make a capsule.
  • red-fleshed apple and cherry plum capsules can also be obtained by a method comprising the following steps:
  • Step 1 Seed or core the red-fleshed apples and cherry plums respectively and then beat;
  • Step 2 Filter the slurry (for example, filter it through 100-300 mesh gauze) to prepare red-fleshed apple and cherry plum juice; in some embodiments, the mesh number of the gauze is 200 mesh;
  • Step 3 freeze-dry or belt-dry the juice into powder
  • Step 4 Mix red-fleshed apple and cherry plum fruit powder in different proportions (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1: 3. 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) prepared into a composition , or in step 1, first mix red-fleshed apple and cherry plum raw materials in different proportions as mentioned above, and then beat to prepare fruit powder;
  • Step 5 Put the fruit powder into the capsule shell to make a capsule.
  • red-fleshed apple and cherry plum capsules can also be prepared from extracts obtained by the aforementioned leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, macroporous resin separation or other extraction methods as raw materials.
  • the present invention selects natural red-fleshed apples and cherry plums, and the prepared red-fleshed apple and cherry plum compositions have good anti-atherosclerotic effects.
  • the effect of the two combinations is better than that of red-fleshed apples or cherry plums alone, and Has a synergistic effect.
  • the effect of the combination of the composition and the drug is better than that of the composition or the drug alone, and has a synergistic effect.
  • the red-fleshed apple and cherry plum products described in this embodiment refer to red-fleshed apple and cherry plum fresh fruits.
  • Red-fleshed apples (Hongxun No. 1) and cherry plums are harvested from 1 month before maturity to when they are fully mature. When harvesting, give priority to picking fruits on sunny days. Select and remove rotten fruits or fruits with poor color, and store them in cold storage after harvesting. .
  • the obtained red-fleshed apples and cherry plums were combined according to different proportions (weight ratio) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1 :2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1), fresh fruits can be eaten directly.
  • the red-fleshed apple and cherry plum products described in this embodiment refer to red-fleshed apple and cherry plum juice cloudy juice.
  • red-fleshed apples (Hongxun No. 1) and cherry plums
  • red-fleshed apple and cherry plum products described in this embodiment refer to red-fleshed apple and cherry plum juice clear juice.
  • red-fleshed apple and cherry plum products described in this embodiment refer to red-fleshed apple and cherry plum jam.
  • Red-fleshed apples (Hongxun No. 1) and cherry plums are seeded or cored, then pulped, boiled and heated to make a thicker jam.
  • the red-fleshed apple and cherry plum products described in this embodiment refer to red-fleshed apple and cherry plum fruit powder.
  • Red-fleshed apples (Hongxun No. 1) and cherry plums are seeded or cored, then pulped, and then freeze-dried to make pulp freeze-dried powder.
  • red-fleshed apple and cherry plum products described in this embodiment refer to red-fleshed apple and cherry plum tablet candies.
  • Red-fleshed apples (Hongxun No. 1) and cherry plums are seeded or cored, then pulped, freeze-dried to make pulp freeze-dried powder, and the pulp freeze-dried powder and auxiliary materials are pressed into tablets to make tablet candy.
  • the red-fleshed apple and cherry plum products described in this embodiment refer to red-fleshed apple and cherry plum water-extracted oral liquids.
  • Red-fleshed apples (Hongxun No. 1) and cherry plums are seeded or cored and then pulped to prepare red-fleshed apple and cherry plum pulp.
  • red-fleshed apple and cherry plum products described in this embodiment refer to red-fleshed apple (Hongxun No. 1) and cherry plum alcohol-extracted oral liquid.
  • red-fleshed apple and cherry plum products described in this embodiment refer to red-fleshed apple (Hongxun No. 3) and cherry plum water-extracted oral liquid.
  • the preparation steps are the same as in Example 7.
  • red-fleshed apple and cherry plum products described in this embodiment refer to red-fleshed apple (Hongxun No. 6) and cherry plum water-extracted oral liquid.
  • the preparation steps are the same as in Example 7.
  • the red-fleshed apple and cherry plum products described in this embodiment refer to red-fleshed apple and cherry plum ultrasonic water-extracted oral liquids.
  • Red-fleshed apples (Hongxun No. 1) and cherry plums are seeded or cored and then pulped to prepare red-fleshed apple and cherry plum pulp.
  • red-fleshed apple and cherry plum products described in this embodiment refer to red-fleshed apple (Hongxun No. 6) and cherry plum ultrasonic water-extracted oral liquid.
  • the preparation steps are the same as in Example 10.
  • red-fleshed apple and cherry plum products described in this embodiment refer to red-fleshed apple (Hongxun No. 1) and cherry plum ultrasonic alcohol extraction oral liquid.
  • the red-fleshed apple and cherry plum products described in this embodiment refer to red-fleshed apple and cherry plum ultra-high pressure water-extracted oral liquids.
  • Red-fleshed apples (Hongxun No. 1) and cherry plums are seeded or cored and then pulped to prepare red-fleshed apple and cherry plum pulp.
  • red-fleshed apple and cherry plum products described in this embodiment refer to red-fleshed apple (Hongxun No. 6) and cherry plum ultra-high pressure water-extracted oral liquid.
  • the preparation steps are the same as in Example 13.
  • red-fleshed apple and cherry plum products described in this embodiment refer to red-fleshed apple (Hongxun No. 1) and cherry plum ultra-high pressure alcohol extracted oral liquid.
  • the red-fleshed apple and cherry plum products described in this embodiment refer to the subcritical water-extracted oral liquid of red-fleshed apple and cherry plum.
  • Red-fleshed apples (Hongxun No. 1) and cherry plums are seeded or cored and then pulped to prepare red-fleshed apple and cherry plum pulp.
  • red-fleshed apple and cherry plum products described in this embodiment refer to red-fleshed apple and cherry plum jam.
  • red-fleshed apple (Hongxun No. 1) and cherry plum water-extracted oral liquids were first prepared, and then boiled and heated to make thicker jam, with or without the addition of auxiliary materials.
  • the red-fleshed apple and cherry plum products described in this embodiment refer to red-fleshed apple and cherry plum fruit powder.
  • red-fleshed apple (Hongxun No. 1) and cherry plum water-extracted oral liquids were first prepared, and then freeze-dried to prepare freeze-dried powder, with or without the addition of auxiliary materials.
  • red-fleshed apple and cherry plum products described in this embodiment refer to red-fleshed apple and cherry plum jam.
  • red-fleshed apple (Hongxun No. 1) and cherry plum ultrasonic extraction oral liquids are first prepared, and then boiled and heated to make thicker jam, with or without the addition of auxiliary materials.
  • the red-fleshed apple and cherry plum products described in this embodiment refer to red-fleshed apple and cherry plum fruit powder.
  • red-fleshed apple (Hongxun No. 1) and cherry plum ultrasonic extraction oral liquids are first prepared, and then belt-dried to make powder, with or without the addition of auxiliary materials.
  • red-fleshed apple and cherry plum products described in this embodiment refer to red-fleshed apple and cherry plum mellow juice products.
  • Example 7 first prepare red-fleshed apple (Hongxun No. 1) and cherry plum water extraction concentrate, and mix it with 95% ethanol for 3 hours.
  • the weight ratio of the concentrate to 95% ethanol is 1:2, and then
  • the alcohol-precipitated clear juice is obtained by filtration and separation, and ethanol is removed by rotary evaporation to obtain a red-fleshed apple and cherry plum composition alcohol-precipitated clear juice product.
  • the red-fleshed apple and cherry plum products described in this embodiment refer to red-fleshed apple and cherry plum macroporous resin separation products.
  • Example 22 To first prepare red-fleshed apple (Hongxun No. 1) and cherry plum pure juice products, using macroporous adsorption
  • the resin adsorbs the above clarified extract, and performs gradient elution with water, 20%, 40%, 60%, and 100% ethanol in sequence.
  • the elution speed is 3BV/h (3 column volumes). Each gradient is collected separately.
  • the eluent; perform rotary evaporation to remove ethanol from the above eluate, and the rotary evaporation temperature is 30-60°C to obtain different macroporous resin separation samples.
  • the atherosclerosis model (i.e. model group) was established after 12 weeks of modeling, and the control group was fed with ordinary feed for 12 weeks (i.e. normal group).
  • the sample administration group was given test samples while modeling, and the normal group and model group were given equal doses of distilled water. After 12 weeks of continuous administration, the formation of atherosclerotic plaques in the mouse aorta was observed, and the plaque area was dissected. Statistical plaque percentage.
  • Red-fleshed apples and cherry plums were extracted according to the water extraction method of Example 7 to obtain red-fleshed apple water leaching extract and cherry plum water leaching extract.
  • Red-fleshed apple extract and cherry plum extract have a certain effect when used alone at a dose of 40g/kg, and when used in combination within the range of 1:10 to 10:1 (composition dose is 40g/kg), both have better effects than the model
  • composition dose is 40g/kg
  • E(A+B) is the inhibition rate of the two drugs combined
  • EA and EB are the inhibition rates of each drug alone.
  • q ⁇ 1 indicates that the two drugs have an antagonistic effect
  • q ⁇ 1 indicates that the two drugs have a synergistic effect.
  • Wild-type C57 mice were used as the normal control group and rosuvastatin was used as the positive drug.
  • the anti-atherosclerotic efficacy of the mixed composition samples was examined in the high-fat diet-induced ApoE-/- mouse atherosclerosis model (i.e. treatment effect).
  • the atherosclerosis model was established after 17 weeks of modeling, and the control group was fed with ordinary feed for 17 weeks.
  • the sample administration group the formation of atherosclerotic plaques in the mouse aorta was observed after 17 weeks of modeling and continuous administration for 4 weeks. The plaque area was dissected and the plaque percentage was calculated.
  • Red-fleshed apples and cherry plums were extracted according to the water extraction method of Example 7, to obtain red-fleshed apple water leaching extracts and cherry plum water leaching extracts.
  • Red-fleshed apple extract and cherry plum extract have a certain effect when used alone at a dose of 40g/kg, and when used in combination within the range of 1:10 to 10:1 (composition dose is 40g/kg), both have better effects than the model
  • composition dose is 40g/kg
  • the efficacy of the mixed composition sample in preventing atherosclerosis was examined in the high-fat diet-induced ApoE-/- mouse atherosclerosis model. 12 weeks of modeling to establish animation Atherosclerosis model (i.e., model group), and the control group were fed with ordinary feed for 12 weeks (i.e., normal group).
  • the sample administration group was given test samples while modeling, and the normal group and model group were given equal doses of distilled water. After 12 weeks of continuous administration, the formation of atherosclerotic plaques in the mouse aorta was observed, and the plaque area was dissected. Statistical plaque percentage.
  • Red-fleshed apple extract and cherry plum extract are less effective when used alone at doses of 20g/kg and 10g/kg respectively, but when used in combination (composition doses are 20g/kg or 10g/kg respectively), they are more effective than the model. Significant effect, can significantly reduce plaque in arterial walls.
  • Wild-type C57 mice were used as the normal control group, and rosuvastatin and atorvastatin were used as positive drugs to investigate the anti-atherogenic effects of mixed composition samples in the high-fat diet-induced ApoE-/- mouse atherosclerosis model. Similar sclerosis effect (i.e. therapeutic effect).
  • the atherosclerosis model was established after 17 weeks of modeling, and the control group was fed with ordinary feed for 17 weeks. Composition samples in After 17 weeks of modeling, the formation of atherosclerotic plaques in the mouse aorta was observed after continuous administration for 4 weeks, and the plaque area was dissected to calculate the plaque percentage.
  • the test results are shown in Table 5.
  • the composition was extracted with reference to Example 7.
  • the red-fleshed apple and cherry plum compositions were in the range of 40g/kg to 10g/kg.
  • the doses of the positive drugs rosuvastatin and atorvastatin are used in combination, the plaque in the arterial wall can be significantly reduced compared to the model group, which has the effect of treating atherosclerosis.
  • the composition has a synergistic effect when used in combination with the drug compared to when used alone (refer to Experimental Example 1 for the calculation method).
  • Wild-type C57 mice were used as the normal control group, and aspirin, clopidogrel, colchicine and probucol were used as positive drugs to investigate the mixed composition in the high-fat diet-induced atherosclerosis model of ApoE-/- mice.
  • Sample anti-atherogenic Sclerosing effect i.e. therapeutic effect.
  • the atherosclerosis model was established after 17 weeks of modeling, and the control group was fed with ordinary feed for 17 weeks. After 17 weeks of modeling, the composition sample was continuously administered for 4 weeks to observe the formation of atherosclerotic plaques in the mouse aorta, and the plaque area was dissected to calculate the plaque percentage.
  • the test results are shown in Table 6, which compares the anti-atherosclerotic effects of different positive drugs when used in combination with the composition.
  • the composition was extracted with reference to Example 7.
  • the red-fleshed apple and cherry plum compositions were compared with the positive drugs aspirin, clopidogrel, and autumn in the range of 40g/kg to 10g/kg.
  • the plaque in the arterial wall can be significantly reduced, which has the effect of treating atherosclerosis.
  • the composition has a synergistic effect when used in combination with the drug compared to when used alone (refer to Experimental Example 1 for the calculation method).
  • ApoE-/- was induced by high-fat diet.
  • the anti-atherosclerotic efficacy (ie, therapeutic effect) of the sample was examined in the mouse atherosclerosis model.
  • the atherosclerosis model was established after 17 weeks of modeling, and the control group was fed with ordinary feed for 17 weeks. After 17 weeks of modeling, the sample group was continuously administered for 4 weeks to observe the formation of atherosclerotic plaques in the mouse aorta, and the plaque area was dissected and the plaque percentage was calculated.
  • Wild-type C57 mice were used as the normal control group and rosuvastatin was used as the positive drug.
  • the anti-atherosclerotic efficacy of the mixed composition samples was examined in the high-fat diet-induced ApoE-/- mouse atherosclerosis model (i.e. treatment effect).
  • the atherosclerosis model was established after 17 weeks of modeling, and the control group was fed with ordinary feed for 17 weeks. After 17 weeks of modeling, the composition sample was continuously administered for 4 weeks to observe the formation of atherosclerotic plaques in the mouse aorta, and the plaque area was dissected to calculate the plaque percentage.
  • Example 8 It can be seen from Table 8 that the compositions prepared by referring to the extraction methods with different ethanol concentrations in Example 7 and Example 8 all have good effects on treating atherosclerosis. It can be seen that red-fleshed apple and cherry plum compositions with different extraction methods have better effects in animal models, and have better effects than when used alone.
  • Table 8 The therapeutic effects of red-fleshed apple and cherry plum compositions in mice on atherosclerosis using different extraction methods
  • the anti-atherosclerotic efficacy of the composition sample i.e., treatment of Effect.
  • the atherosclerosis model was established after 17 weeks of modeling, and the control group was fed with ordinary feed for 17 weeks. After 17 weeks of modeling, the composition sample was continuously administered for 4 weeks to observe the formation of atherosclerotic plaques in the mouse aorta. The mice were dissected and the plaque area (percentage) was counted.
  • compositions prepared by referring to the macroporous resin separation method of gradient elution with different ethanol concentrations in Example 22 and Example 23 all have good effects on treating atherosclerosis. It can be seen that red-fleshed apple and cherry plum compositions with different extraction methods have better effects in animal models, and have better effects than using them alone.

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Abstract

La présente invention concerne une composition de Malus niedzwetzkyana Dieck et de Prunus cerasifera Ehrhart, le rapport pondéral entre Malus niedzwetzkyana Dieck et Prunus cerasifera Ehrhart étant de 1:10-10:1. La composition a un effet synergique dans la prévention ou le traitement de l'athérosclérose. La composition de Malus niedzwetzkyana Dieck et de Prunus cerasifera Ehrhart peut être utilisée sous forme de jus, de confitures, de poudres de fruit, de produits liquides oraux, de gélules, de comprimés, etc..
PCT/CN2023/092926 2022-05-13 2023-05-09 Composition de malus niedzwetzkyana dieck et de prunus cerasifera ehrhart et leur utilisation en termes de résistance à l'athérosclérose WO2023217115A1 (fr)

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