WO2022041352A1 - Additif alimentaire pour prévenir la diarrhée et améliorer l'immunité, son procédé de préparation et son application - Google Patents
Additif alimentaire pour prévenir la diarrhée et améliorer l'immunité, son procédé de préparation et son application Download PDFInfo
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- WO2022041352A1 WO2022041352A1 PCT/CN2020/116377 CN2020116377W WO2022041352A1 WO 2022041352 A1 WO2022041352 A1 WO 2022041352A1 CN 2020116377 W CN2020116377 W CN 2020116377W WO 2022041352 A1 WO2022041352 A1 WO 2022041352A1
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- WIPO (PCT)
- Prior art keywords
- feed additive
- parts
- improving immunity
- diarrhea
- preventing diarrhea
- Prior art date
Links
- 206010012735 Diarrhoea Diseases 0.000 title claims abstract description 67
- 239000003674 animal food additive Substances 0.000 title claims abstract description 56
- 230000036039 immunity Effects 0.000 title claims abstract description 54
- 238000002360 preparation method Methods 0.000 title abstract description 8
- 241001465754 Metazoa Species 0.000 claims abstract description 22
- 239000000843 powder Substances 0.000 claims abstract description 20
- 239000006041 probiotic Substances 0.000 claims abstract description 17
- 235000018291 probiotics Nutrition 0.000 claims abstract description 17
- -1 compound amino acids Chemical class 0.000 claims abstract description 13
- 230000000529 probiotic effect Effects 0.000 claims abstract description 11
- PUKLDDOGISCFCP-JSQCKWNTSA-N 21-Deoxycortisone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2=O PUKLDDOGISCFCP-JSQCKWNTSA-N 0.000 claims abstract description 7
- 229920000858 Cyclodextrin Polymers 0.000 claims abstract description 7
- 239000001116 FEMA 4028 Substances 0.000 claims abstract description 7
- FCYKAQOGGFGCMD-UHFFFAOYSA-N Fulvic acid Natural products O1C2=CC(O)=C(O)C(C(O)=O)=C2C(=O)C2=C1CC(C)(O)OC2 FCYKAQOGGFGCMD-UHFFFAOYSA-N 0.000 claims abstract description 7
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 claims abstract description 7
- 235000011175 beta-cyclodextrine Nutrition 0.000 claims abstract description 7
- 229960004853 betadex Drugs 0.000 claims abstract description 7
- 239000002509 fulvic acid Substances 0.000 claims abstract description 7
- 229940095100 fulvic acid Drugs 0.000 claims abstract description 7
- 229920001661 Chitosan Polymers 0.000 claims abstract description 6
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 claims abstract description 5
- 229960003966 nicotinamide Drugs 0.000 claims abstract description 4
- 235000005152 nicotinamide Nutrition 0.000 claims abstract description 4
- 239000011570 nicotinamide Substances 0.000 claims abstract description 4
- 235000001014 amino acid Nutrition 0.000 claims description 14
- 244000063299 Bacillus subtilis Species 0.000 claims description 11
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 11
- 241000194108 Bacillus licheniformis Species 0.000 claims description 10
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 10
- 239000002994 raw material Substances 0.000 claims description 10
- 241000193749 Bacillus coagulans Species 0.000 claims description 9
- 229940054340 bacillus coagulans Drugs 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 8
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims description 6
- 239000004473 Threonine Substances 0.000 claims description 6
- 239000004480 active ingredient Substances 0.000 claims description 6
- 239000004471 Glycine Substances 0.000 claims description 5
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 5
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 4
- 235000013922 glutamic acid Nutrition 0.000 claims description 4
- 239000004220 glutamic acid Substances 0.000 claims description 4
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 abstract description 10
- 244000144977 poultry Species 0.000 abstract description 7
- 230000009286 beneficial effect Effects 0.000 abstract description 6
- 239000003242 anti bacterial agent Substances 0.000 abstract description 5
- 229940088710 antibiotic agent Drugs 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- DFPAKSUCGFBDDF-ZQBYOMGUSA-N [14c]-nicotinamide Chemical compound N[14C](=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-ZQBYOMGUSA-N 0.000 abstract description 3
- 244000144972 livestock Species 0.000 abstract description 3
- 239000003814 drug Substances 0.000 abstract description 2
- 239000003381 stabilizer Substances 0.000 abstract description 2
- 229940045110 chitosan Drugs 0.000 abstract 2
- 239000000654 additive Substances 0.000 abstract 1
- 230000000996 additive effect Effects 0.000 abstract 1
- 229940079593 drug Drugs 0.000 abstract 1
- 230000000091 immunopotentiator Effects 0.000 abstract 1
- 241000287828 Gallus gallus Species 0.000 description 30
- 235000013330 chicken meat Nutrition 0.000 description 30
- 230000000694 effects Effects 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 6
- 235000013594 poultry meat Nutrition 0.000 description 6
- 235000013305 food Nutrition 0.000 description 5
- 230000001717 pathogenic effect Effects 0.000 description 5
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 210000001035 gastrointestinal tract Anatomy 0.000 description 4
- 230000003053 immunization Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 230000003203 everyday effect Effects 0.000 description 3
- 206010061126 Escherichia infection Diseases 0.000 description 2
- 102000003886 Glycoproteins Human genes 0.000 description 2
- 108090000288 Glycoproteins Proteins 0.000 description 2
- 102000015728 Mucins Human genes 0.000 description 2
- 108010063954 Mucins Proteins 0.000 description 2
- 238000010171 animal model Methods 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000002158 endotoxin Substances 0.000 description 2
- 208000020612 escherichia coli infection Diseases 0.000 description 2
- 238000013401 experimental design Methods 0.000 description 2
- 230000008642 heat stress Effects 0.000 description 2
- 230000028993 immune response Effects 0.000 description 2
- 238000002649 immunization Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 210000000936 intestine Anatomy 0.000 description 2
- 230000003902 lesion Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 229940051875 mucins Drugs 0.000 description 2
- 235000021062 nutrient metabolism Nutrition 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 230000035882 stress Effects 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000007059 acute toxicity Effects 0.000 description 1
- 231100000403 acute toxicity Toxicity 0.000 description 1
- 235000019728 animal nutrition Nutrition 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000000022 bacteriostatic agent Substances 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 235000021245 dietary protein Nutrition 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 229930195712 glutamate Natural products 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229940072322 hylan Drugs 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000036542 oxidative stress Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000016515 regulation of signal transduction Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- 238000009602 toxicology test Methods 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
Definitions
- the invention belongs to the technical field of animal husbandry and veterinary medicine, and relates to a feed additive and a preparation method thereof, in particular to a feed additive for preventing diarrhea and improving immunity, and a preparation method and application thereof.
- the purpose of the present invention is to provide a feed additive for preventing diarrhea and improving immunity, so as to solve the problem of poor resistance of livestock and poultry when the medicated feed additive is not used;
- Another object of the present invention is to provide a preparation method of the above-mentioned feed additive for preventing diarrhea and improving immunity;
- Another object of the present invention is to provide an application of the above-mentioned feed additive for preventing diarrhea and improving immunity.
- a feed additive for preventing diarrhea and improving immunity, in parts by weight, the raw materials for making its active ingredients include: 0.086-0.36 parts of compound amino acids, 3-6 parts of probiotic bacteria powder, 1-3 parts of nicotinamide, shell 0.5-2 parts of polysaccharide, 1-3 parts of ⁇ -cyclodextrin and 0.5-2 parts of fulvic acid.
- the raw materials for making the active ingredient of the compound amino acid include: 1-12 parts of glutamic acid, 6-18 parts of threonine and 1.6-6 parts of glycine.
- the raw materials for making the active ingredients of the probiotic powder include: 2-4 parts of Bacillus subtilis powder, 1-3 parts of Bacillus licheniformis powder and Bacillus coagulans 1 to 3 servings of powder;
- Bacillus subtilis was purchased from Wuxi Byford Biotechnology Co., Ltd.;
- Bacillus licheniformis was purchased from Jiangsu Yuanshan Biotechnology Co., Ltd.;
- Bacillus coagulans was purchased from Jiangsu Yuanshan Biotechnology Co., Ltd.;
- probiotics are used in the present invention, but are not limited to the probiotics of the above-mentioned companies. It should be understood that the probiotics involved in the technical solution claimed in the present invention are all probiotics of the same species and genus with the same performance.
- the feed additive for preventing diarrhea and improving immunity contains Bacillus subtilis ⁇ 1.0 ⁇ 10 6 CFU/g, Bacillus licheniformis addition amount ⁇ 1.0 ⁇ 10 6 CFU/g, Bacillus coagulans ⁇ 1.0 ⁇ 10 6 CFU/g.
- the present invention also provides a method for preparing the above-mentioned feed additive for preventing diarrhea and improving immunity.
- the present invention also provides an application of the above-mentioned feed additive for preventing diarrhea and improving immunity, characterized in that the feed additive for preventing diarrhea and improving immunity is used for feeding animals to improve immunity and prevent/treat diarrhea.
- the feed additive for preventing diarrhea and improving immunity is used to add to animal feed.
- the present invention can effectively improve animal health by adding compound amino acids, probiotic bacteria powder, immune enhancers (chitosan and fulvic acid), and stabilizers (nicotinamide and ⁇ -cyclodextrin) in a reasonable proportion to keep the beneficial bacteria stable.
- immune enhancers chitosan and fulvic acid
- stabilizers nicotinamide and ⁇ -cyclodextrin
- the compound amino acid contains glutamic acid, threonine and glycine.
- Glutamate is an important component of food protein, and can play an important role in nutrient metabolism, energy supply, immune response, oxidative stress and regulation of signaling pathways.
- Threonine as an essential amino acid in animal growth, plays an important role in animal nutrition metabolism, immune response and other processes.
- mucus composed of glycoproteins and mucins is rich in threonine.
- Glycine plays an important role in nutrient metabolism, energy supply and other processes, and also has the effect of antagonizing endotoxin and heat stress. While providing the body with the required amino acids, compound amino acids can also resist heat stress, increase the corresponding immune protein content, and then improve production performance.
- Compound amino acids can also promote the growth and colonization of probiotics; the three amino acids cooperate with each other to achieve anti-oxidative stress and signal pathway regulation, help the production of mucosal immune components composed of glycoproteins and mucins in the body, and have the ability to antagonize bacterial endotoxins and anti-heat stress effect. But beyond the ratio of the present invention or using other amino acids can not play the above-mentioned effect well;
- the probiotic powder contains Bacillus subtilis, Bacillus licheniformis and Bacillus subtilis, which can effectively kill pathogenic bacteria in the intestinal tract, quickly colonize the intestinal tract, form a biofilm, protect the intestinal tract, and stimulate the corresponding non-specific immune function; Bacillus subtilis can consume oxygen in the intestinal tract, produce bacteriostatic substances, and has bactericidal effect; Bacillus licheniformis has the effect of producing enzymes and can promote metabolism; Bacillus coagulans can regulate intestinal flora imbalance.
- the three kinds of probiotics cooperate with each other to achieve the above effects, and the three kinds of bacteria contained in the prepared feed additive for preventing diarrhea and improving immunity are suitable for storage at room temperature, which further reduces the cost of clinical use;
- Niacinamide can provide the body with the required vitamins, and at the same time, it can maintain the stability of beneficial bacteria in the intestine, promote the proliferation of beneficial bacteria, improve the body's non-specific immunity, and enhance specific immunity;
- Chitosan and fulvic acid can also maintain the stability of beneficial bacteria in the intestine, promote the proliferation of beneficial bacteria, and improve the body's non-specific and specific immunity;
- ⁇ -cyclodextrin can coat and stabilize each component and maintain the stability of compound amino acid and probiotic powder
- the added components of the present invention are green and environmentally friendly, have good effects after use, and have no residue, can replace the pharmaceutical feed additive to enhance animal immunity, reduce animal diarrhea rate, especially can enhance the immunity of poultry animals, reduce the diarrhea rate of poultry animals ;
- the feed additive of the invention can replace some antibiotics, and while implementing the national policy, the loss of the breeding industry is reduced, the harm caused by the medicinal feed additive to the human body through the food chain is reduced, and the food safety is escorted;
- the invention has wide raw material sources and low cost, and can bring good economic benefits to breeding enterprises.
- the feed additive for preventing diarrhea and improving immunity of the present invention is used for adding to animal feed to feed animals, and is especially suitable for feeding poultry animals.
- Embodiment 1 A kind of preparation method of the feed additive that prevents diarrhea and improves immunity
- the present embodiment prepares the feed additive for preventing diarrhea and improving immunity, including the following steps that are carried out in sequence:
- auxiliary material M1 Take 16kg of compound amino acids, 500kg of probiotic bacteria powder and 484kg of auxiliary material M1, and mix them evenly to obtain a feed additive (marked as N1) for preventing diarrhea and improving immunity, which contains 2.0 ⁇ 10 8 CFU/g Bacillus subtilis, 1.0 ⁇ 10 7 CFU/g Bacillus licheniformis, 2.0 ⁇ 10 7 CFU/g Bacillus coagulans.
- the feed additive for preventing diarrhea and improving immunity prepared in this example is used to add to animal feed for feeding animals, especially for feeding poultry animals.
- Embodiments 2 to 6 are respectively a preparation method of a feed additive for preventing diarrhea and improving immunity, and their steps are basically the same as those in Example 1, and the difference is only in the amount of raw materials. For details, see Table 1:
- Table 1 List of raw material consumption in each step of feed additive for preventing diarrhea and improving immunity
- Example 7 Application of feed additive for preventing diarrhea and improving immunity
- the feed additives N1 to N6 for preventing diarrhea and improving immunity provided in Examples 1 to 6 are respectively added to the compound feed of common commercially available laying hens during the peak laying period, and the mixed chicken feeds are marked as SN11 to SN16 in one-to-one correspondence. , the addition amount of feed additives N1-N6 is 0.5% of the feed weight.
- This experiment was conducted in a large chicken farm with diarrhea in Hebei province. Selected 3500 300-day-old Hylan gray laying hens with diarrhea symptoms to ensure the same diarrhea rate, and were randomly divided into 7 groups with 500 hens in each group, namely the control group and A1-A6 groups. Among them, the control group was fed the ordinary city The laying hens were sold with compound feed during the peak egg production period. Groups A1 to A6 were fed chicken feeds SN11 to SN16 respectively, and were fed unlimited amounts throughout the day. The trial period starts from April 20, 2020 to April 27, 2020.
- the experiment was carried out in three-layer three-dimensional cages, fed with corresponding feed, and had free access to food and water. According to the routine immunization program of Hyland gray laying hens, the immunization and feeding management are carried out daily, and the chicken house is disinfected every day to ensure the hygiene of the chicken house. During the test period, the diarrhea situation was observed every day, and the diarrhea rate was statistically calculated. The specific results are shown in the following table:
- the feed additives N1 to N6 for preventing diarrhea and improving immunity provided by Examples 1 to 6 are respectively added to the compound feed of ordinary commercial laying hens during the peak laying period, and the mixed chicken feeds are marked as SN21 to SN26 in one-to-one correspondence. , the addition amount of feed additives N1-N6 is 0.5% of the feed weight.
- the experiment was carried out in three-layer three-dimensional cages, fed with corresponding feed, and had free access to food and water. According to the routine immunization program of Hyland gray laying hens, the immunization and feeding management are carried out, and the chicken house is disinfected every day to ensure the hygiene of the chicken house.
- Example 8 Acute Toxicology Test of Feed Additives for Preventing Diarrhea and Improving Immunity
- the feed additive N1 for preventing diarrhea and improving immunity provided by Example 1 is added to the SPF chicken brooding period feed in the normal amount, 10 times the amount, 50 times the amount and 100 times the amount respectively, and the mixed chicken feeds correspond one by one. Labeled as SPFN1 to SPFN4, the normal amount is the feed additive N1 added at 0.5% of the weight of the SPF chicken feed.
- Fifty 21-day-old SPF chickens were selected and randomly divided into 5 groups with 10 chickens in each group, namely the control group and the C1-C4 groups. Among them, the control group was fed the SPF chicken brooding period feed, and the C1-C4 groups were fed the chickens respectively. Feed SPFN1 ⁇ SPFN4, unlimited feeding throughout the day. The trial period is from May 11, 2020 to October 25, 2020. During the feeding process, the clinical response of the chickens was observed and recorded. After the feeding, the organ lesions of the SPF chickens in each group were necropsied. The results showed that the control group and the C1-C4 groups, the various indicators of the SPF chickens were normal, and there were no lesions. Phenomenon. The above experimental results prove that the feed additive for preventing diarrhea and improving immunity prepared by the present invention has no acute toxicity.
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Environmental Sciences (AREA)
- Birds (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Fodder In General (AREA)
Abstract
L'invention concerne un additif alimentaire destiné à prévenir la diarrhée et à améliorer l'immunité, son procédé de préparation et son application, se rapportant au domaine technique de l'élevage et de la médecine vétérinaire. L'additif alimentaire est préparé par mélange uniforme d'acides aminés composés, de poudre probiotique, de nicotinamide, de chitosane, de β-cyclodextrine et d'acide fulvique ; l'additif préparé est utilisé pour être ajouté aux aliments pour animaux et est approprié pour l'alimentation d'animaux, en particulier pour l'alimentation d'animaux de type volaille. En ajoutant un rapport raisonnable des acides aminés composés, de la poudre probiotique, d'immunopotentialisateurs (le chitosane et l'acide fulvique) et de stabilisants pour conserver la stabilité des bactéries bénéfiques (le niacinamide et la β-cyclodextrine), l'immunité des animaux peut être efficacement améliorée, la diarrhée peut être empêchée/traitée, les performances de production peuvent être améliorées, et l'utilisation d'antibiotiques est réduite/remplacée, ce qui permet de résoudre le problème de faible résistance du bétail lorsque des additifs d'alimentation de type médicament ne sont pas utilisés.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010870913.4 | 2020-08-26 | ||
| CN202010870913.4A CN114097937A (zh) | 2020-08-26 | 2020-08-26 | 预防腹泻提高免疫力的饲料添加剂及其制备方法和应用 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2022041352A1 true WO2022041352A1 (fr) | 2022-03-03 |
Family
ID=80352514
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/CN2020/116377 WO2022041352A1 (fr) | 2020-08-26 | 2020-09-21 | Additif alimentaire pour prévenir la diarrhée et améliorer l'immunité, son procédé de préparation et son application |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN114097937A (fr) |
| WO (1) | WO2022041352A1 (fr) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114874940A (zh) * | 2022-05-10 | 2022-08-09 | 吉林省农业科学院 | 一种复合菌剂及其发酵物和应用 |
| CN115152893A (zh) * | 2022-07-04 | 2022-10-11 | 广西农业职业技术大学 | 一种清远麻羽雏鸡用复合益生菌组合物及其制备方法 |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006064449A1 (fr) * | 2004-12-15 | 2006-06-22 | Van Der Westhuizen Cornelis Fl | Composition detoxifiante et stimulant l'immunite |
| CN102805209A (zh) * | 2012-08-30 | 2012-12-05 | 湖南省微生物研究所 | 一种畜禽用合生素饲料添加剂及其应用 |
| CN104522433A (zh) * | 2015-01-08 | 2015-04-22 | 北京北农企业管理有限公司 | 一种蛋鸡用复合生物饲料添加剂及其制备方法 |
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| CN111436542A (zh) * | 2020-04-02 | 2020-07-24 | 安徽天隆饲料有限公司 | 替代抗生素预防雏鸡腹泻提高生长性饲料添加剂及其应用 |
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| CN114874940A (zh) * | 2022-05-10 | 2022-08-09 | 吉林省农业科学院 | 一种复合菌剂及其发酵物和应用 |
| CN115152893A (zh) * | 2022-07-04 | 2022-10-11 | 广西农业职业技术大学 | 一种清远麻羽雏鸡用复合益生菌组合物及其制备方法 |
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