WO2022005255A3 - Pna 프로브를 이용한 코로나바이러스감염증-19을 일으키는 sars-cov-2 및 살베코바이러스의 동시 진단방법 및 진단용 키트 - Google Patents

Pna 프로브를 이용한 코로나바이러스감염증-19을 일으키는 sars-cov-2 및 살베코바이러스의 동시 진단방법 및 진단용 키트 Download PDF

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WO2022005255A3
WO2022005255A3 PCT/KR2021/008444 KR2021008444W WO2022005255A3 WO 2022005255 A3 WO2022005255 A3 WO 2022005255A3 KR 2021008444 W KR2021008444 W KR 2021008444W WO 2022005255 A3 WO2022005255 A3 WO 2022005255A3
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sarbecovirus
coronavirus
gene
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sars
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WO2022005255A2 (ko
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이시석
김경탁
양은주
이한우
박희경
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주식회사 시선바이오머티리얼스
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Publication of WO2022005255A2 publication Critical patent/WO2022005255A2/ko
Publication of WO2022005255A3 publication Critical patent/WO2022005255A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

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Abstract

본 발명은 역전사 중합효소 연쇄반응(Reverse transcription Real-time polymerase chain reaction)을 이용한 베타코로나바이러스의 하속(subgenus)중 하나인 살베코바이러스(Sarbecovirus) 및 코로나바이러스감염증-19을 일으키는 신종 코로나바이러스(Severe acute respiratory syndrome coronavirus 2, SARS-CoV-2)를 검출하는 방법에 관한 것으로, 더욱 자세하게 살베코바이러스 및 신종 코로나바이러스의 동시 검출용 PNA 프로브와 프라이머 및 이를 이용한 감염여부 판별방법에 관한 것으로, 본 발명에 따르면, 종래 코로나바이러스감염증-19 진단에 사용되는 DNA 프로브를 이용한 PCR과 RT-PCR 방법에 비해 민감도와 특이도가 높으며 신종 코로나바이러스의 ORF1ab 유전자, S 유전자, N 유전자 및 살베코바이러스의 E 유전자를 동시 증폭 및 판별할 수 있어, 고민감도와 높은 정확성을 가지고 신종 코로나바이러스 및 살베코바이러스의 감염 여부분석 가능하다.
PCT/KR2021/008444 2020-07-03 2021-07-02 Pna 프로브를 이용한 코로나바이러스감염증-19을 일으키는 sars-cov-2 및 살베코바이러스의 동시 진단방법 및 진단용 키트 WO2022005255A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2020-0082335 2020-07-03
KR1020200082335A KR102367980B1 (ko) 2020-07-03 2020-07-03 PNA 프로브를 이용한 코로나바이러스감염증-19을 일으키는 SARS-CoV-2 및 살베코바이러스의 동시 진단방법 및 진단용 키트

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WO2022005255A3 true WO2022005255A3 (ko) 2022-02-17

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KR102624632B1 (ko) 2022-03-11 2024-01-11 성균관대학교산학협력단 SARS-CoV-2 감염 질환 중증도 진단 또는 예후 예측용 바이오마커
KR102500714B1 (ko) 2022-09-30 2023-02-17 주식회사 학메디칼 코로나 바이러스 감염 진단을 위한 검체 채취용 조성물 및 이를 이용한 코로나바이러스 감염 진단 키트

Citations (2)

* Cited by examiner, † Cited by third party
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KR101916899B1 (ko) * 2017-08-31 2018-11-08 한국생명공학연구원 Sars 관련 코로나바이러스 및 mers 관련 코로나바이러스 동시 검출용 프라이머 및 이를 이용한 검출 방법
CN111139317A (zh) * 2020-03-13 2020-05-12 欧陆分析技术服务(苏州)有限公司 一种sars-cov-2病毒多重荧光定量pcr检测试剂盒及检测方法

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KR101916899B1 (ko) * 2017-08-31 2018-11-08 한국생명공학연구원 Sars 관련 코로나바이러스 및 mers 관련 코로나바이러스 동시 검출용 프라이머 및 이를 이용한 검출 방법
CN111139317A (zh) * 2020-03-13 2020-05-12 欧陆分析技术服务(苏州)有限公司 一种sars-cov-2病毒多重荧光定量pcr检测试剂盒及检测方法

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ANONYMOUS: "Multiplex Real-Time PCR for detection of SARS-CoV-2 using TaqPath COVID-19 Combo Kit (Applied Biosystems)", 7 April 2020 (2020-04-07), XP055900412, Retrieved from the Internet <URL:https://www.icmr.gov.in/pdf/covid/labs/SARS_CoV2_using_TaqPath_COVID19_ComboKit.pdf> *
ISHIGE TAKAYUKI; MURATA SHOTA; TANIGUCHI TOSHIBUMI; MIYABE AKIKO; KITAMURA KOUICHI; KAWASAKI KENJI; NISHIMURA MOTOI; IGARI HIDETOS: "Highly sensitive detection of SARS-CoV-2 RNA by multiplex rRT-PCR for molecular diagnosis of COVID-19 by clinical laboratories", CLINICA CHIMICA ACTA, ELSEVIER BV, AMSTERDAM, NL, vol. 507, 23 April 2020 (2020-04-23), AMSTERDAM, NL , pages 139 - 142, XP086157986, ISSN: 0009-8981, DOI: 10.1016/j.cca.2020.04.023 *
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KR20220004468A (ko) 2022-01-11
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