ZA202305277B - Primers, kit and method for detecting of african swine fever virus - Google Patents

Primers, kit and method for detecting of african swine fever virus

Info

Publication number
ZA202305277B
ZA202305277B ZA2023/05277A ZA202305277A ZA202305277B ZA 202305277 B ZA202305277 B ZA 202305277B ZA 2023/05277 A ZA2023/05277 A ZA 2023/05277A ZA 202305277 A ZA202305277 A ZA 202305277A ZA 202305277 B ZA202305277 B ZA 202305277B
Authority
ZA
South Africa
Prior art keywords
primers
kit
swine fever
fever virus
african swine
Prior art date
Application number
ZA2023/05277A
Inventor
Jintao Li
Xiaoyan Ding
Jiuxiang He
Yuxin Zhou
Xiaoyang Zhou
Original Assignee
Univ Army Medical
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Army Medical filed Critical Univ Army Medical
Publication of ZA202305277B publication Critical patent/ZA202305277B/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Primers, a kit and a method for detecting African swine fever virus are provided, which relates to virus detection technologies. The primers are used to detect vp72 gene of African swine fever virus, and nucleotide sequences thereof are shown in SEQ ID NO: 6 and SEQID NO: 7. The kit includes the primers. The method uses the primers or the kit to perform dye fluorescence quantitative polymerase chain reaction (PCR) on genomic DNA of a sample to be detected. This method has strong specificity, high sensitivity, good reproducibility, and lower cost than the TaqMan probe method.
ZA2023/05277A 2021-12-03 2023-05-15 Primers, kit and method for detecting of african swine fever virus ZA202305277B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202111470170 2021-12-03
CN202210536550.XA CN114807444A (en) 2021-12-03 2022-05-17 Primer, kit and method for detecting African swine fever virus

Publications (1)

Publication Number Publication Date
ZA202305277B true ZA202305277B (en) 2023-12-20

Family

ID=82516143

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA2023/05277A ZA202305277B (en) 2021-12-03 2023-05-15 Primers, kit and method for detecting of african swine fever virus

Country Status (3)

Country Link
US (1) US20230374614A1 (en)
CN (1) CN114807444A (en)
ZA (1) ZA202305277B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117511936B (en) * 2024-01-05 2024-04-09 广州奕昕生物科技有限公司 Kit and method for detecting highly pathogenic porcine reproductive and respiratory syndrome virus based on MB-RT-PCR
CN117965806A (en) * 2024-01-17 2024-05-03 上海雄图生物科技有限公司 Cross-species multiple pathogen multiplex amplification system and detection kit

Also Published As

Publication number Publication date
CN114807444A (en) 2022-07-29
US20230374614A1 (en) 2023-11-23

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