WO2021203648A1 - Traditional chinese medicine composition for preventing gingival atrophy and/or treating gingivitis - Google Patents

Abstract

Disclosed is a traditional Chinese medicine composition for preventing gingival atrophy and/or treating gingivitis. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 5-7 parts of rhizoma chuanxiong, 6-9 parts of rhizoma imperatae, 4-6 parts of and herba agrimoniae, and 2-4 parts of radix astragali. The traditional Chinese medicine composition has precise compatibility and a synergistic effect, can prevent redness and swelling and bleeding of the gum, gingival atrophy and other symptoms, and can effectively treat gingivitis. The composition can be applied to preparation of toothpaste, mouth wash and other oral products, and has a good market prospect.

Description

Chinese medicinal composition for preventing gum atrophy and/or treating gingivitis Technical field

The invention specifically relates to a traditional Chinese medicine composition for preventing gingival recession and/or treating gingivitis.

Background technique

Gum is one of the periodontal tissues (gingiva, periodontal ligament, alveolar bone, cementum), directly exposed to the oral cavity, visible directly, it is composed of keratinized epithelium and connective tissue, covering the alveolar bone and Tooth root. Gum disease is a lesion confined to the gingival tissue, the most common of which is chronic gingivitis, also known as marginal gingivitis and simple gingivitis. It belongs to "gingivitis only related to plaque" and is plaque gum The most common disease in inflammation. Inflammation of the gums is mainly located in the free gums and gingival papillae, which can occur in children between 3 and 5 years old. The prevalence and severity of the disease gradually increase with age, reaching a peak in adolescence. Gingivitis is slightly more male than female in children. Developing countries and regions are higher than economically developed countries.

"Pujifang·Upper Sore Gate" mentioned: "Lack of qi and blood, no way to wipe out, wind and evil attacking the deficiency, guest between the teeth, it will make the muscles cold and blood weak, the teeth will shrink and the flesh will be exposed, and it will gradually be exposed. It does not adhere to the root of the tooth.” It is pointed out that when the body's qi and blood are weak, the Yangming meridian of the hands and feet cannot be used to infuse sperm. This shows that insufficient Qi and blood can lead to periodontal disease.

At present, there are many reports on drug research on the treatment of periodontal disease. Among them, traditional Chinese medicine composition has become the focus of research as a kind of drug with high safety. However, there is no relevant report on traditional Chinese medicine composition with less medicinal taste, low cost and significant effect.

Summary of the invention

In order to solve the above-mentioned problems, the present invention provides a traditional Chinese medicine composition for preventing gingival recession and/or treating gingivitis, which is prepared from raw materials with the following weight ratios:

5-7 parts of Ligusticum chuanxiong, 6-9 parts of Imperata cylindrica, 4-6 parts of Agrimony, 2-4 parts of Astragalus.

Further, it is prepared from raw materials with the following weight ratios:

5 parts of Chuanxiong, 6 parts of Imperata cylindrica, 4 parts of Agrimony, 3 parts of Astragalus.

Further, it is a preparation prepared from the powder of the raw material drug, or the water or organic solvent extract of the raw material drug as the active ingredient, plus the acceptable auxiliary materials on the medicine or cosmetics.

Furthermore, the formulation is an oral care product formulation.

Furthermore, the oral care product preparation is powder, toothpaste or mouthwash.

The present invention also provides a method for preparing the aforementioned Chinese medicine composition. The preparation of the extract includes the following steps:

1) Weigh the raw materials according to the ratio;

2) Mix and crush the raw materials, add water and decoct twice, add 9-10 times the amount of water for one decoction, soak for 40-50min, keep slightly boiling for 50-60min after boiling, filter; add 7-8 times for the second decoction Measure the water, keep it boiling for 40-50 minutes after boiling, filter, combine the two filtrates, and concentrate the filtrate under reduced pressure at 60-65°C to a relative density of 1.20-1.30 to obtain an extract;

3) Take step 2) and vacuum dry the extract at 60±5°C until the surface is brittle and hard, without wet powder, and it is obtained by ultrafine pulverization.

The present invention also provides a method for preparing the aforementioned Chinese medicine composition. The preparation of the powder includes the following steps:

a. Weigh the raw materials according to the ratio;

b Mix and pulverize the raw materials, pass a 100-mesh sieve to obtain powder; mix and grind borneol and mint ice with a mass ratio of 3:2 to obtain ice powder;

c Mix the medicated powder and ice powder in a ratio of 18:5, and get it.

The present invention also provides a method for preparing the aforementioned traditional Chinese medicine composition. The mouthwash is prepared by dissolving the aforementioned extract in an aqueous solution with a mass percentage of 8%;

The aqueous solution is also dissolved in mass percentage 5.2% glycerol, 3.1% sorbitol, 0.5% PEG-40 hydrogenated castor oil, 2.0% tea extract, 0.5% trichlorogalactose, 1.2% propylene glycol, 0.3% sodium citrate, 0.1% lactic acid, 2.0% xylitol, 0.5% menthol, 0.1% glyceryl caprylate, 0.4% sodium saccharin, 0.1% disodium EDTA, 0.02% flavor and 0.1% methyl paraben.

Further, the tea extract is green tea extract.

Finally, the present invention provides a use of the aforementioned traditional Chinese medicine composition in the preparation of a medicine for preventing gingival atrophy.

The present invention finally provides a use of the aforementioned traditional Chinese medicine composition in the preparation of a medicine for treating gingivitis.

Ligusticum chuanxiong is derived from the dried rhizomes of Ligusticum chuanxiong Hort., an umbelliferous plant Ligusticum chuanxiong Hort. It is often used for promoting blood circulation and promoting qi, dispelling wind and relieving pain. Ligusticum chuanxiongxin is warm and fragrant. It can not only disperse and reach the top when walking without keeping it; it also enters the blood points and reaches the sea of blood when it descends. It has a wide range of activating blood and removing stasis. Antibacterial and antiviral effects. It can inhibit inflammation and treat infectious diseases.

Imperata cylindrica Root is the dried rhizome of the grass family Imperata cylindrica Beauv.var.major (Nees) C.E.Hubb. It has the effect of cooling blood to stop bleeding, clearing heat and diuresis. Used for blood-heat vomiting, epistaxis, hematuria, fever polydipsia, jaundice, edema, hot shower astringent pain; acute nephritis and edema. Imperata cylindrica root can enhance the hemostatic effect by affecting the blood coagulation system and platelet aggregation. At the same time, it also has broad-spectrum antibacterial and anti-inflammatory and analgesic effects.

Agrimonia is the dry above-ground part of Agrimonia pilosa Ledeb, a Rosaceae plant. It has the effects of astringent, stop bleeding, stop malaria, stop diarrhea, and detoxify. It is clinically used for various hemorrhages. Modern medicine has confirmed that Agrimonia vulgaris has coagulation, astringent hemostasis, antibacterial, analgesic and anti-inflammatory effects. The hemostatic effect is through inhibiting the production of NO, increasing the number of platelets, and accelerating intraplatelet coagulation. Realized by the release of substances.

Astragalus is the dry root of legume Astragalus membranaceus (Fisch.) Bge.var.mongholicus (Bge.) Hsiao or Astragalus membranaceus (Fisch.) Bge. The main effects are tonifying qi and solidifying the surface, supporting toxins and purging pus, diuresis and muscle growth. It is used for qi deficiency, fatigue, chronic diarrhea, prolapse of the anus, spontaneous sweating, edema, uterine prolapse, chronic nephritis, proteinuria, diabetes, and long-term unhealing of sores. Modern pharmacology shows that Astragalus can promote the body's antibody production, enhance the body's immune function, increase the activity of natural killer cells, and enhance the phagocytic function of phagocytes. It can be used for chronic infectious diseases.

In the pharmaceutical composition of the present invention, Ligusticum chuanxiong and Imperata cylindrica root are the monarch medicine, Agrimonia serrata is the minister medicine, astragalus is the adjuvant medicine, Ligusticum chuanxiong is invigorating blood and promoting qi, can reach the top of the blood, and enter the blood at the bottom to reach the blood sea; Cooling blood to stop bleeding can enhance hemostasis by affecting the coagulation system and platelet aggregation. It also has broad-spectrum antibacterial, anti-inflammatory and analgesic effects. Agrimonia converges to stop bleeding, antibacterial and analgesic; together with astragalus to invigorate qi and solidify the surface, support toxins and drain pus, and grow muscles. The combined use of various drugs has the effects of nourishing qi, nourishing blood, strengthening teeth, nourishing gums, hemostasis and antibacterial. It can effectively treat gingivitis and prevent gum swelling, bleeding, and gum atrophy.

Clinical trials have proved that the pharmaceutical composition of the present invention has precise compatibility and synergistic effect, can effectively treat gingivitis, and prevent symptoms such as gingival swelling, bleeding, and gingival atrophy. It can be used to prepare toothpaste, mouthwash and other oral products, and has a good market prospect.

Obviously, according to the above-mentioned content of the present invention, according to common technical knowledge and conventional means in the field, various other modifications, substitutions or alterations can be made without departing from the above-mentioned basic technical idea of the present invention.

Hereinafter, the above-mentioned content of the present invention will be further described in detail through specific implementations in the form of examples. However, it should not be understood that the scope of the above-mentioned subject of the present invention is limited to the following examples. All technologies implemented based on the foregoing content of the present invention belong to the scope of the present invention.

Detailed ways

Example 1: Preparation of the drug of the present invention

Formula: 5 parts of Ligusticum chuanxiong, 6 parts of Imperata cylindrica, 4 parts of Agrimony, 3 parts of Astragalus.

Preparation:

(1) Weigh the API according to the formula stated in the formula;

(2) Mix and crush the raw materials and place them in a frying pan. Add water to decoct 2 times. Add 9 times the amount of water to one decoction, soak for 40 minutes, decoct, and keep it boiling for 50 minutes after boiling, then filter; add 7 times for the second decoction Measure the water, keep it boiled for 40 minutes after boiling, filter, and combine the two filtrates;

(3) The filtrate is concentrated under reduced pressure at 65°C to an extract with a density of 1.20 to 1.30;

(4) The extract is vacuum dried at 60±5℃, and the material is collected when the surface is brittle and hard without wet powder;

(5) The collected materials are superfinely pulverized to obtain the active ingredient extract I of the drug combination.

Example 2: Preparation of the drug of the present invention

Formula: Ligusticum chuanxiong 7g, Imperata cylindrica 9g, Agrimony 6g, Astragalus 4g.

Preparation:

(1) Weigh the API according to the formula stated in the formula;

(2) Mix and crush the raw materials and place them in a frying pan. Add water to decoct 2 times. Add 10 times the amount of water to one decoction, soak for 50 minutes, decoct, and keep it boiled for 60 minutes after boiling, then filter; add 8 times for the second decoction Measure the water, keep it boiling for 45 minutes after boiling, filter, and combine the two filtrates;

(3) The filtrate is concentrated under reduced pressure at a temperature of 60 to 65°C to an extract with a density of 1.20 to 1.30;

(4) The extract is vacuum dried at 60±5℃, and the material is collected when the surface is brittle and hard without wet powder;

(5) The collected materials are superfinely pulverized to obtain the active ingredient extract II of the drug combination.

Example 3: Preparation of the drug of the present invention

Formula: Chuanxiong 6g, Imperata chinensis root 8g, Agrimonia 5g, Astragalus 2g.

Preparation:

(1) Weigh the API according to the formula stated in the formula;

(2) Mix and crush the raw materials and place them in a frying pan. Add water to decoct 2 times. Add 10 times the amount of water to one decoction, soak for 50 minutes, decoct, and keep it boiled for 60 minutes after boiling, then filter; add 8 times for the second decoction Measure the water, keep it boiling for 45 minutes after boiling, filter, and combine the two filtrates;

(3) The filtrate is concentrated under reduced pressure at a temperature of 60 to 65°C to an extract with a density of 1.20 to 1.30;

(4) The extract is vacuum dried at 60±5℃, and the material is collected when the surface is brittle and hard without wet powder;

(5) The collected materials are superfinely pulverized to obtain the active ingredient extract III of the drug combination.

Example 4: Preparation of powder I for treating gingivitis of the present invention

Chuanxiong 5g, Imperata cylindrica 6g, Agrimony 4g, Astragalus 3g, Borneol 3g, Peppermint ice 2g.

(1) After pulverizing the above medicinal materials separately, they are passed through a 100-mesh sieve;

(2) Weigh Chuanxiong, Imperata cylindrica root, Agrimonia, and Astragalus according to the formula described in the formula, place them in a mortar, grind and pour out for later use;

(3) Lightly grind the borneol and mint in a mortar with ice, and after grinding, add the Ligusticum chuanxiong mixed powder, and grind until the color is uniform, and it is ready.

Example 5: Preparation of mouthwash for preventing gingivitis of the present invention

1) Take the traditional Chinese medicine extract I prepared in Example 1, deionized water, glycerin, sorbitol, PEG-40 hydrogenated castor oil, tea extract, trichlorogalactose, propylene glycol, sodium citrate, lactic acid, xylitol, Menthol, glyceryl caprylate, sodium saccharin, disodium EDTA, flavor and methyl paraben;

2) Mix and dissolve the above-mentioned raw materials to prepare a solution with the following mass percentages:

Deionized water 75.88%, glycerin 5.2%, sorbitol 3.1%, PEG-40 hydrogenated castor oil 0.5%, green tea extract 2.0%, traditional Chinese medicine extract I8.0%, trichlorogalactose 0.5%, propylene glycol 1.2%, lemon Sodium acid 0.3%, lactic acid 0.1%, xylitol 2.0%, menthol 0.5%, glyceryl caprylate 0.1%, saccharin sodium 0.4%, EDTA disodium 0.1%, flavor 0.02%, methyl paraben 0.1%.

The mouthwash liquid obtained above is put into the mouthwash packaging bag in a sterile tank to obtain it.

The beneficial effects of the present invention are illustrated below through comparative examples and test examples.

Comparative example 1:

Recipe: 5 parts of Ligusticum chuanxiong and 6 parts of Imperata cylindrica.

Preparation:

(1) Weigh the API according to the formula stated in the formula;

(2) Mix and crush the raw materials and place them in a frying pan. Add water to decoct 2 times. Add 9 times the amount of water to one decoction, soak for 40 minutes, decoct, and keep it boiling for 50 minutes after boiling, then filter; add 7 times for the second decoction Measure the water, keep it boiled for 40 minutes after boiling, filter, and combine the two filtrates;

(3) The filtrate is concentrated under reduced pressure at 65°C to an extract with a density of 1.2 to 1.30;

(4) The extract is vacuum dried at 60±5℃, and the material is collected when the surface is brittle and hard without wet powder;

(5) Superfine the collected materials to obtain the active ingredient extract IV of the drug combination.

Comparative example 2:

Formula: 4 parts of Agrimony and 3 parts of Astragalus.

Preparation:

(1) Weigh the API according to the formula stated in the formula;

(2) Mix and crush the raw materials and place them in a frying pan. Add water to decoct 2 times. Add 9 times the amount of water to one decoction, soak for 40 minutes, decoct, and keep it boiling for 50 minutes after boiling, then filter; add 7 times for the second decoction Measure the water, keep it boiled for 40 minutes after boiling, filter, and combine the two filtrates;

(3) The filtrate is concentrated under reduced pressure at 65°C to an extract with a density of 1.20 to 1.30;

(4) The extract is vacuum dried at 60±5℃, and the material is collected when the surface is brittle and hard without wet powder;

(5) The collected materials are superfinely pulverized to obtain the active ingredient extract V of the drug combination.

Comparative example 3:

Recipe: 4 servings of Agrimony.

Preparation:

(1) Weigh the API according to the formula stated in the formula;

(2) Mix and crush the raw materials and place them in a frying pan. Add water to decoct 2 times. Add 9 times the amount of water to one decoction, soak for 40 minutes, decoct, and keep it boiling for 50 minutes after boiling, then filter; add 7 times for the second decoction Measure the water, keep it boiled for 40 minutes after boiling, filter, and combine the two filtrates;

(3) The filtrate is concentrated under reduced pressure at 65°C to an extract with a density of 1.20 to 1.30;

(4) The extract is vacuum dried at 60±5℃, and the material is collected when the surface is brittle and hard without wet powder;

(5) Superfine the collected materials to obtain the active ingredient extract VI of the drug combination.

Comparative example 4:

Recipe: 3 servings of Astragalus.

Preparation:

(1) Weigh the API according to the formula stated in the formula;

(2) Mix and crush the raw materials and place them in a frying pan. Add water to decoct 2 times. Add 9 times the amount of water to one decoction, soak for 40 minutes, decoct, and keep it boiling for 50 minutes after boiling, then filter; add 7 times for the second decoction Measure the water, keep it boiled for 40 minutes after boiling, filter, and combine the two filtrates;

(3) The filtrate is concentrated under reduced pressure at 65°C to an extract with a density of 1.20 to 1.30;

(4) The extract is dried in vacuum at 60±5℃, and the materials are collected when the surface is brittle and hard without wet powder;

(5) The collected materials are subjected to ultrafine pulverization to obtain the active ingredient extract of the drug combination VII.

Test Example 1: Detection of active ingredients

Take the active ingredient extract I of the above-mentioned Example 1, the active ingredient extract IV of the comparative example 1, the active ingredient extract V of the comparative example 2, the active ingredient extract VI of the comparative example 3, and the active ingredient extract of the comparative example 4 Ⅶ, carry out the detection of active ingredients, among which the detection methods of ferulic acid in Ligusticum chuanxiong and astragaloside IV in astragalus are determined according to the 2020 pharmacopoeia high performance liquid chromatography (General Principle 0512), and the detection method of chlorogenic acid in Imperata cylindrica root is according to Zhang Suhong .The quality standards of Imperata cylindrica root and Polygonatum odoratum are researched and tested. The detection method of agricol in agrimony is determined by Du Chao. Show.

Table 1

To	Ferulic acid	Chlorogenic acid	Atriol	Astragaloside IV
Active ingredient extract Ⅰ	0.0821	0.0934	0.0375	0.0735
Active ingredient extract Ⅳ	0.0432	0.0282	/	/
Active ingredient extract Ⅴ	/	/	0.0089	0.0375
Active ingredient extract Ⅵ	/	/	0.0025	/
Active ingredient extract Ⅶ	/	/	/	0.0424

From Table 1 above, it can be known that using the formula of the present invention: 5 parts of Ligusticum chuanxiong, 6 parts of Imperata cylindrica root, 4 parts of Agrimony, 3 parts of Astragalus, can better improve the effective substances of ferulic acid, chlorogenic acid, and crane grass. The content of phenol and astragaloside IV can achieve the purpose of synergy.

Comparative Example 5: Preparation of Comparative Gingivitis Powder II

Ligusticum chuanxiong 5g, Imperata cylindrica 6g, borneol 3g, mint ice 2g.

(1) After pulverizing the above medicinal materials separately, they are passed through a 100-mesh sieve;

(2) Weigh Chuanxiong and Imperata cylindrica roots in a mortar according to the formula stated in the formula.

(3) Lightly grind the borneol and mint in a mortar with ice, and after grinding, add the Ligusticum chuanxiong mixed powder, and grind until the color is uniform, and it is ready.

Comparative Example 6: Preparation of Comparative Gingivitis Powder Ⅲ

Ligusticum chuanxiong 5g, Imperata cylindrica 6g, Agrimony 4g, Borneol 3g, Mint ice 2g.

(1) After pulverizing the above medicinal materials separately, they are passed through a 100-mesh sieve;

(2) Weigh Ligusticum chuanxiong, Imperata cylindrica root, Agrimonia serrata according to the formula described in the formula, put it in a mortar, grind and pour out for later use;

(3) Lightly grind the borneol and mint in a mortar with ice, and after grinding, add the Ligusticum chuanxiong mixed powder, and grind until the color is uniform, and it is ready.

Comparative Example 7: Preparation of Comparative Gingivitis Powder IV

Ligusticum chuanxiong 5g, Imperata cylindrica root 6g, Astragalus root 3g, Borneol 3g, Peppermint ice 2g.

(1) After pulverizing the above medicinal materials separately, they are passed through a 100-mesh sieve;

(2) Weigh Chuanxiong, Imperata cylindrica, and Astragalus according to the ratio described in the formula, place them in a mortar, grind and pour out for later use;

(3) Lightly grind the borneol and mint in a mortar with ice, and after grinding, add the Ligusticum chuanxiong mixed powder, and grind until the color is uniform, and it is ready.

Test Example 2: Clinical test results of gingivitis powder

1. Clinical data

We selected 100 patients with gingivitis who were treated in the Department of Stomatology, Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Sichuan Province from January 2018 to June 2018. Among them, 47 were males and 53 were females, aged (28-56) years old, with an average age of 42 years old

1.1 Inclusion criteria

①Diagnosed as a patient with gingivitis in the Department of Stomatology, Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, with ≥10 existing true teeth;

②The patient is healthy, without systemic diseases, such as blood diseases, diabetes, etc., and the female is not pregnant or breast-feeding;

③Age between 18 and 60 years old;

④ Have not received periodontal treatment and have not taken antibiotics and non-steroidal anti-inflammatory drugs in the past six months;

⑤I and my family members have the knowledge of this research and signed the consent form.

1.2 Exclusion criteria

① Accompanied by serious systemic diseases or malignant tumors;

②Those who have mental illness and cannot communicate normally;

③ Those who have used antibiotics within 3 months before treatment;

④Incomplete clinical data;

⑤Pregnant women and women who are breastfeeding;

⑥Those who are allergic to the tested product.

2. Treatment methods

This product is applied for external use. After cleaning, take appropriate amount of powder I of Example 4, powder II of Comparative Example 5, powder III of Comparative Example 6, and powder IV of Comparative Example 7 to apply to the affected area of the gums. 3 times a day, for long-term chronic or severe cases, 4-5 times a day, do not directly apply to the eyes, ears or damaged skin (such as unhealed wounds, infections, ulcers or erosive wounds). One week is a course of treatment, using 3 to 4 courses of treatment.

3. Criteria for judging curative effect

①Healed: The redness and pain of the gums disappeared, the bleeding stopped, and there was no recurrence for more than half a year;

②Effective: reduce the redness and pain of the gums, and reduce the number of bleeding;

③Invalid: the gums are swollen and painful and the bleeding of the gums does not improve or even worsens.

Total effective rate=(cure+effective) number of cases/total number of cases×100%.

4. Therapeutic effect

To	Healed (case)	Effective (example)	Invalid (example)	Total effective rate
Example 4	6	17	2	92%
Comparative example 5	1	12	12	52%
Comparative example 6	0	9	16	36%
Comparative example 7	0	7	16	28%

The test result shows that after the product of the present invention is used for 2 courses, the redness and pain of the gums are reduced, and the number of bleeding is reduced; after 3 to 4 courses, the redness and pain of the gums disappears and the bleeding stops.

Test example 3:

1. Clinical data

We selected 102 patients with gingivitis who were treated in the Department of Stomatology, Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Sichuan Province from May 2018 to November 2018. Among them, 43 were males and 59 were females, aged (16-50) years old, with an average age of 31 years old

1.1 Inclusion criteria

①Diagnosed as a patient with gingivitis in the Department of Stomatology, Affiliated Hospital of Chengdu University of Traditional Chinese Medicine.

②Persons and their family members who have the knowledge of this research and have signed the consent form.

③Age between 16 and 50 years old.

④No other organ diseases, no obvious secondary infection.

1.2 Exclusion criteria

① Accompanied by serious systemic diseases or malignant tumors;

②Those who have mental illness and cannot communicate normally;

③ Those who have used antibiotics within 3 months before treatment;

④ Patients with incomplete clinical data.

⑤Pregnant women and women who are breastfeeding;

⑥Those who are allergic to the tested product.

2. Treatment methods

This product is applied for external application. After cleaning, take an appropriate amount of the extract I prepared in Example 1 and apply it to the affected area of the gums. 3 times a day, for long-term chronic or severe cases, 4-5 times a day, do not directly apply to the eyes, ears or damaged skin (such as unhealed wounds, infections, ulcers or erosive wounds). One week is a course of treatment, using 1 to 3 courses of treatment.

3. Criteria for judging curative effect

①Healed: The redness and pain of the gums disappeared, the bleeding stopped, and there was no recurrence for more than half a year;

②Effective: reduce the redness and pain of the gums, and reduce the number of bleeding;

③Invalid: the gums are swollen and painful and the bleeding of the gums does not improve or even worsens.

Total effective rate=(cure+effective) number of cases/total number of cases×100%.

4. Treatment results

To	Healed (case)	Effective (example)	Invalid (example)	Total effective rate
Example 1	73	twenty three	6	94.1%

The test results explained: After using the product for 1 course, the swelling and pain of the gums were reduced, and the number of bleeding was reduced; after using the product for 2 to 3 courses, the swelling and pain of the gums disappeared and the bleeding stopped.

In summary, the Chinese medicine composition of the present invention has precise compatibility and synergistic effect, can effectively treat gingivitis, and prevent symptoms such as gingival swelling and bleeding. It can be used to prepare toothpaste, mouthwash and other oral products, and has a good market prospect.

Claims (11)

1. A traditional Chinese medicine composition for preventing gingival recession and/or treating gingivitis, which is characterized in that it is prepared from raw materials with the following weight ratios:

   5-7 parts of Ligusticum chuanxiong, 6-9 parts of Imperata cylindrica, 4-6 parts of Agrimony, 2-4 parts of Astragalus.
2. The traditional Chinese medicine composition according to claim 1, characterized in that it is prepared from the following raw materials with the following weight ratio:

   5 parts of Chuanxiong, 6 parts of Imperata cylindrica, 4 parts of Agrimony, 3 parts of Astragalus.
3. The traditional Chinese medicine composition according to claim 1 or 2, characterized in that: it is prepared from the powder of the raw material drug, or the water or organic solvent extract of the raw material drug as the active ingredient, plus pharmaceutical or cosmetic acceptable auxiliary materials The preparations made.
4. The traditional Chinese medicine composition according to any one of claims 1 to 3, wherein the preparation is an oral care product preparation.
5. The traditional Chinese medicine composition according to claim 4, wherein the oral care product preparation is powder, toothpaste or mouthwash.
6. A method for preparing the traditional Chinese medicine composition according to claim 3, characterized in that: the preparation of the extract comprises the following steps:

   1) Weigh the API according to claim 1;

   2) Mix and crush the raw materials, add water and decoct twice, add 9-10 times the amount of water for one decoction, soak for 40-50min, keep slightly boiling for 50-60min after boiling, filter; add 7-8 times for the second decoction Measure the water, keep it boiling for 40-50 minutes after boiling, filter, combine the two filtrates, and concentrate the filtrate under reduced pressure at 60-65°C to a relative density of 1.20-1.30 to obtain an extract;

   3) Take step 2) and vacuum dry the extract at 60±5°C until the surface is brittle and hard, without wet powder, and it is obtained by ultrafine pulverization.
7. A method for preparing the traditional Chinese medicine composition according to claim 5, characterized in that: the preparation of the powder comprises the following steps:

   a. Weigh the raw materials according to the ratio of claim 1;

   b Mix and pulverize the raw materials, pass a 100-mesh sieve to obtain powder; mix and grind borneol and mint ice with a mass ratio of 3:2 to obtain ice powder;

   c Mix the medicated powder and ice powder in a ratio of 18:5, and get it.
8. A preparation method of the traditional Chinese medicine composition according to claim 5, characterized in that: the mouthwash is made by dissolving the extract prepared according to claim 6 in an aqueous solution with a mass percentage of 8%;

   The aqueous solution is also dissolved in mass percentage 5.2% glycerol, 3.1% sorbitol, 0.5% PEG-40 hydrogenated castor oil, 2.0% tea extract, 0.5% trichlorogalactose, 1.2% propylene glycol, 0.3% sodium citrate, 0.1% lactic acid, 2.0% xylitol, 0.5% menthol, 0.1% glyceryl caprylate, 0.4% sodium saccharin, 0.1% disodium EDTA, 0.02% flavor and 0.1% methyl paraben.
9. The preparation method according to claim 8, wherein the tea extract is green tea extract.
10. Use of the traditional Chinese medicine composition according to any one of claims 1 to 5 in the preparation of a medicine for preventing gingival recession.
11. Use of the traditional Chinese medicine composition according to any one of claims 1 to 5 in the preparation of a medicament for the treatment of gingivitis.