KR101345709B1 - Antibacterial and antifungal composition and Ladies genital area cleanser composition comprising antibacterial and antifungal composition for preventing and treating vaginitis - Google Patents

Abstract

The present invention relates to a natural extract antimicrobial composition and a cleaning composition for women effective in the prevention or treatment of vaginitis using the same, and more specifically, to a composition mixed with extracts of red ginseng, red ginseng, casualties, branch, golden, yellow lotus and yellow white In more detail, the present invention relates to a natural composition exhibiting an antimicrobial effect on the causative agent of vaginitis and a female cleansing agent composition for pharmaceuticals, cosmetics, detergents or wet wipes comprising the same. The herbal extracts of red ginseng, red ginseng, casualties, branch, golden, yellow lotus and yellow white components of the present invention are eco-friendly herbal extracts extracted from nature and can be applied without any side effects and toxicity in the application of the human body. On the other hand, the present invention has excellent antibacterial and antifungal effects, and in particular, it exhibits excellent antibacterial activity against Candida albicans, a fungus that causes vaginitis, and Gardnerella Vaginalis, a common bacterium, to prevent vaginitis and It is suitable for use for therapeutic purposes. In addition, excellent formulation can be prepared in a variety of formulations it is possible to commercialize the product according to the taste and convenience of the patient.

Description

Antibacterial and antifungal composition and Ladies genital area cleanser composition comprising antibacterial and antifungal composition for preventing and treating vaginitis

The present invention relates to a natural extract antimicrobial composition having an antibacterial and antifungal effect and a cleaning composition for women using the same, and more particularly, Candida albicans, a fungus that causes vaginitis, and Gardnerella pansal, which is a general bacterium. The present invention relates to a natural extractive antimicrobial composition excellent in antimicrobial activity to Gardnerella Vaginalis and a feminine detergent composition prepared using the natural extractive antimicrobial composition.

The vagina of the woman is the connection between the vulva and the uterus and is the most humid and warm place in the woman's body. Therefore, because of the environment suitable for bacteria or fungi, which are the main causes of vaginitis, the disease is likely to occur. Under normal conditions, many lactic acid bacteria live inside the vagina, making the inside of the vagina weakly acidic and inhibiting the growth of pathogens and fungi.However, long-term antibiotic use, long-term use of birth control pills, tampons or loops, or pregnancy, childbirth, lactation or menopause When the balance in the vagina is broken due to a change in the balance of hormones, vaginitis may develop due to the proliferation of opportunistic pathogens in which growth is normally inhibited under normal conditions in the vagina. Vaginitis has been reported with bacterial vaginosis, Candida vaginitis, Trichomonas vaginitis, Atrophic vaginitis, and the like.

The most common disease is bacterial vaginosis, which is caused by Gardnerella vaginalis. This vaginitis is caused by an imbalance of acidity in the vagina, resulting in the reduction of lactic acid bacteria in the vagina, while overproliferation of anaerobic bacteria, which were less than 1%. There is no known cause of changes in normal vaginal flora, but repeated alkalizing of the vagina due to frequent sexual intercourse or vaginal lavage is believed to play a role. When lactic acid bacteria that produce hydrogen peroxide, which should be normally present, are lost, bacterial vaginitis recurs frequently because regeneration of normal vaginal flora is difficult. In addition, women with bacterial vaginitis have a higher incidence of severe sequelae, including pelvic inflammatory disease (PID), post-abortion PID, postoperative cuff cellulitis, and abnormal cervical pap smear. Premature labor, premature birth, chorioamnionitis, and endometritis after cesarean section are risks.

Candida vaginitis, a common disease along with bacterial vaginitis, is a disease caused by the fungus Candida albicans, which is inflamed in the wet puddle, which affects the vagina at least once during approximately 75% of women's lives. 45% of women who suffer from candida vaginitis in the vulva are a common disease that recurs more than once a year. Candida albicans, which normally holds in the oral cavity, intestine, and vagina of a healthy person, has abnormal immunity due to colds, diabetes, and AIDS when the condition of female hormones is abnormal due to pregnancy or long-term use of oral contraceptives. When you do, it causes an infection. It can also be caused by the use of corticosteroids, which are used to treat obesity, antibiotic allergies, and arthritis. Outbreaks caused by the administration of antibiotics are caused by the killing of beneficial lactic acid bacteria in the vagina, with the possibility of relapse. If you wear too tight girdle or pants, you may not be able to breathe well, or you may wear wet panties because you do not wipe the vulva well after bathing. Only 50% of women with Candida albicans in the vagina have symptoms. The main symptom is cold and itchy skin due to inflammatory reactions, and skin peeling or pus depending on the type and severity of the disease or secondary bacterial infection. There is a difference such as coming out. A characteristic symptom is a dark, white discharge. The vulva is very sensitive to the nerves, and sometimes swelling and inflammation. Burning sensation, dyspareunia, swelling, vulva pain, etc. Clinical findings include vulvar and vaginal erythema, congestion, epidermal detachment, vesicles, pustules.

In order to prevent such female diseases, detergents (vaginal cleaners) and vulva cleaners are often used. Local detergents generally used to prevent vaginitis include sulphonated fatty acids or distilled lauryl alcohol and transparent or roshon synthesized with emollients, thickeners, nutrients, foaming agents, organic fungicides, etc. lotion type has been shipped. In addition, there are also surfactants having detergency and povidone-iodine as pharmaceuticals. Povidone-iodine, a chemical disinfectant, is known to be effective in the prevention and treatment of Candida vaginitis, Trichomonas vaginitis, and other vaginal infections. It is known to cause contact dermatitis, and it is known that an increase in the level of inorganic iodine or total iodine in the vagina may cause hypothyroidism. Conventional techniques for mitigating the side effects of the use of disinfectants as medicines as described above, in healthy women, the acidity of vaginal acid is maintained at pH3.2 by lactic acid secretion by vaginal beneficial microorganisms, and at such low acidity most of the Based on the fact that harmful bacteria inhibit or proliferate, a pH 3.5 buffer solution formulation with lactose as a main ingredient and lactoserum extracted from milk is proposed, but this is normal to vaginal pH rather than feminine cleaner. It is an intravaginal titer for maintaining vaginal condition, and it is difficult to expect fundamental treatment and prevention. In addition, as a general treatment of vaginitis, polyene drugs include nyststin and amphotericinB. Azole drugs include imidazole drugs such as ketoconazole and miconazole, and triazole drugs such as itraconazole and fluconazole.

Even if such antibiotics are appropriate antibiotics, when administered in an insufficient amount, the germs do not die and, due to their resistance, cause greater difficulty in subsequent treatment. In addition, the physicochemical vaginal cleaning agent used as a cleaning agent has limitations such as deterioration of the cleaning effect over a long period of time, the increase of fungi instead of the removal of various bacteria, and the removal of odors and unpleasant odors. Even if many of the above methods have been developed, there is a problem that recurrence occurs well.

On the other hand, to compensate for the shortcomings of the yeast infection prevention and treatment, sanitary napkin coating composition (Korean Patent Laid-Open Publication No. 2001-0014909) and sanitary pad pad (Korean Patent Laid-Open Publication No. 2002-0062474) prepared by using the herbal medicine, local using agar Detergent for cleaning (Korean Patent Patent No. 1979-0001672) is disclosed, and easy to clean and remove the mucosa on the mucosal region with respect to the composition for women's local cleansing (Korean Patent Application No. 10-2004-0072503), Women's cleaning composition comprising bamboo salt and complex herbal extract (Korean Patent Application No. 10-20040085458), vaginal cleanser composition containing pomegranate extract and its preparation method (Korea Patent Application No. 10-2004-0085204), prevention of vaginitis and Method of extracting effective ingredient useful for treatment and cleaning composition containing extract extracted using same (Korean Patent Publication No. 10- 2003-004587), Propolis-containing female cleansing composition and preparation method thereof (Korean Patent Application No. 10-1999-0037078), herbal composition for female local cleansing including red ginseng, leafy leaf, peony and red ginseng (Korean Patent Application No. 10 -2009-0023172 is disclosed. In addition to the conventional compositions as described above, various kinds of compositions for female topical cleaning are disclosed. As described above, various drugs have been proposed as a method for vaginal cleansing and treatment of abnormal symptoms, but there is still insufficient presentation of drugs without irritation considering that the site of use is the most sensitive mucosal site for foreign substances. Therefore, the development of a new technology to meet the continuous demand for a composition using a natural extract without irritation and side effects is required.

The present invention is to solve the above problems, by using natural extracts based on our folk medicine or traditional Chinese medicine, not only shows excellent sterilizing ability against vaginitis causative bacteria, high stability, sterilization of vaginitis causative bacteria In addition, to provide an antimicrobial composition having an antibacterial and antifungal effect consisting of natural extracts that function to preserve the pH in the vagina to preserve lactic acid bacteria in the vagina. In addition, by using human-friendly natural extracts, unlike conventional chemicals with side effects, it is excellent in the prevention and treatment of symptoms by reducing the side effects and irritation, reducing the lesions of patients with vaginitis, and preventing the factors that occur continuously. When applied to a process for commercializing the superiority of the effect or to a female detergent composition such as a detergent having various formulations including the antimicrobial composition of the present invention, there is no effect degradation at the convenience of processing and application of the formulation, The present invention provides a composition in which formulations may be manufactured and commercialized to be applied and used accordingly, so that various formulations may be conveniently used by a vaginitis patient.

In order to achieve the above object, the present invention provides an antimicrobial composition having an antibacterial and antifungal effect, including a natural extract with less side effects and toxicity. More specifically, the present invention provides an antimicrobial composition having an antibacterial and antifungal effect comprising a mixture of herbal extracts of red ginseng, red ginseng, casualty, branch, yellow and white, golden and rhubarb as herbal medicines.

In addition, in order to achieve the above object, the present invention provides a cleaning composition for women comprising the antimicrobial composition as an active ingredient.

The female detergent composition may be to be applied for cleaning, pharmaceutical, cosmetics, wet wipes.

Hereinafter, the present invention will be described in more detail.

In the present invention, the pharmacological effects of the raw herbal medicines of the extract are as follows.

Sohora Root is a perennial herb that grows on the foothills or sunny meadows of the legumes, on the plains, on the roads, on the sandy grounds, and in the sunny loess. Ginseng soothes the itching of the skin, and in <Consciousness Bogam>, hot dog wind causes boils on the skin and flesh and treats eyebrows falling out with redness. It helps to lower the fever and makes you sleep better, brightening your eyes and breaking your tears. Giving a tidbit, heals the dysentery and urine from the latent heat of yellow and red. It is said to heal toothaches, swellings, and cracks in the vagina.

Salvia Miltiorrhiza Root, which is widely used for gynecological diseases, is the root of Salvia miltiorrhiza Bunge (Lactaceae and Labiatae), with short and rough roots and usually leaves stems on the apical tip. Water extract of Salvia Miltiorrhiza (1: 3) has inhibitory effect on various skin fungi and it is tonic treatment, pain relief medicine, pain medicine, anti-inflammatory medicine, blood medicine, menstrual irregularity, embryo pain, menopause, postpartum embryo , Writes on rheumatism. It is said to be effective for treating gynecological diseases such as Samul-tang even if the roots are eaten.

The casualty (Cnidi Fructus) is the fruit of the bee venom (Cnidium monieri (L). Cuss) or the casualty (Torilis japonica Decandolle). It is recorded that the genitals of the woman treat the swelling and pain of the genitals, and the wet and itchy symptoms of the genital area, and it is known to be effective on genital pruritus, skin pruritus, and the treatment of women.

Branch (Kochiae fructus) is the mature fruit of Kochia scoparia Schrader. The branch governs gonorrhea and the treatment of women, and is also applied to skin diseases caused by moist fever, and is known to control pruritus and eczema.

Golden (Scutellaria Root) is the bark of the bark of Scutellaria baicalensis Georgi. It is used as antipyretic, diuretic, branch, edema and anti-inflammatory. It has excellent antibacterial activity. In motion therapy, inflammatory drugs and fever-lowering drugs are used for congestion, diseases with inflammation and fever, embryonic pain, diarrhea, respiratory infections and coughs, moist fever, and inflammatory conjunctivitis.

Colidis (Coptidis Rhizoma) is a rhizome that almost eliminates the roots of Colidis (Coptis japonica Makino) or other similar plants (Ranunculus Ranunculaceae). It is used for various inflammations and is useful for the treatment of dermatitis.In motion therapy, it is a bitterness placebo, sedative, and inflammatory medicine with congestion and inflammatory disease, palpitation and mental anxiety, embryonic pain, diarrhea, dysentery and bleeding. Write on your symptoms.

Phellodendron Bark is the bark of the bark of the Phellodendron amurense Ruprecht or other plant of the same species (Rhyang and Rutaceae). It is an antimicrobial agent that removes moisture and heat. The herbaceous wood is said to cure women's abortions and treats, and to treat vulva skin tissues with swelling. It can also be used for dermatitis, mucositis in the mouth, burns, and cuts.

In the present invention, the mixture of the herbal extracts may be a mixture of red ginseng extract, salvia extract, filamentous extract, branch extract, golden extract, rhubarb extract and sulfur white extract, and also the herbal medicines such as ginseng, dansam, The mixture consisting of casualties, branch, golden, rhubarb and yellow white may be extracted in a batch. If each of the extracts is mixed, the extract may be a purer extract than the components of each extract are prepared, In this case, there may be a slight difference in the effects of the various components are extracted together.

On the other hand, the mixture of the herbal extract is high ginseng extract: red ginseng extract: casualty extract: branch extract: golden extract: rhubarb extract: yellow white extract 1.0 ~ 4.0: 20 ~ 40: 1.0 ~ 4.0: 1.0 ~ 4.0: 40 ~ 60: 5.0 It is preferable to be comprised and mixed by the weight ratio of -15: 1.0-4.0. In addition, the mixture of the herbal extracts of red ginseng: Dansam: casualties: branch: golden: yellow lotus: yellow white 1.0 ~ 4.0: 20 ~ 40: 1.0 ~ 4.0: 1.0 ~ 4.0: 40 ~ 60: 5.0 ~ 15: 1.0 ~ 4.0 It is preferable to mix and extract so that it may consist of weight ratio. At this time, each component ratio of the herbal medicine is preferably used in a similar range as the overall balance ratio, it can have an excellent antibacterial and antifungal effect within the range of this value.

The extraction solvent of the present invention may be used alone or in combination of two or more selected from the group consisting of purified water, ethyl acetate, butyl acetate, methyl alcohol, propanol, isopropyl alcohol, and butyl alcohol. At this time, the extraction temperature is preferably 10 ~ 80 ℃, can be extracted for 1 hour to 24 hours. If the extraction temperature and extraction time is out of the extraction efficiency may decrease or change of components may occur.

The herbal medicine of the present invention may be prepared according to a conventional method of preparing herbal medicine extracts, and specifically, may be a cold extraction method, a hot extraction method or a heat extraction method, and a conventional extraction device or an ultrasonic mill extractor may be used.

The prepared extract can then be filtered or concentrated or dried to remove the solvent, it can be carried out both filtration, concentration and drying. Specifically, the filtration may be performed using a filter paper or a reduced pressure filter, and the concentration may be concentrated under reduced pressure using a reduced pressure concentrator, for example, a rotary evaporator, and the drying may be performed by, for example, a lyophilization method.

In addition, the herbal extract may be one that completely removes the moisture through the obtained extract through concentration and lyophilization, the herbal extract completely removed the moisture can be used in powder form.

On the other hand, the natural extract antimicrobial composition as an example of the present invention comprises: 1) washing and drying step of washing and drying the red ginseng, salvia, casualty, branch, golden, yellow lotus and yellow white as herbal medicines; 2) Extract to obtain an extract by immersion in the extraction solvent consisting of 5 to 10 parts by weight of 25 to 35% concentration alcohols, reflux cooling at 10 to 80 ℃ for 1 to 24 parts by weight of each ingredient of the herbal medicine step; 3) The obtained ginseng extract 1.0 to 4.0: Salvia extract 20 to 40: casualty extract 1.0 to 4.0: branch extract 1.0 to 4.0: golden extract 40 to 60: yellow lotus extract 5.0 to 15: sulfur white extract 1.0 to 4.0 weight ratio (w / w) mixing and mixing the composition; 4) the extract after the mixing step to remove the insoluble material by centrifugation to remove only the supernatant and filtered under reduced pressure, concentrated step of concentrating at 10 ~ 80 ℃ using a reduced pressure concentrator; natural extract antifungal composition prepared Can be.

At this time, the reflux cooling time in step 2) is preferably 2 to 12 hours, more preferably 4 to 8 hours, the alcohol is ethyl acetate, butyl acetate, methyl alcohol, propanol, isopropyl One or more selected from the group consisting of alcohols and butyl alcohols may be used alone or in combination.

In addition, the present invention further comprises a powdering step of obtaining a dry powder by freeze-drying for 24 to 48 hours from -20 ℃ to 30 ℃ step by step after the concentration of 4), characterized in that the natural extract antimicrobial composition It may be a manufacturing method of.

In addition, in extracting the same as the extract of herbal medicines extract ginseng, sweet ginseng, casualties, branch, golden, rhubarb and sulfur white, respectively, by extracting each of the extracts can be mixed with each extract to prepare a natural extract antimicrobial composition of the present invention It is also possible to prepare a natural extract antimicrobial composition of the present invention by extracting a mixture consisting of, salvia, casualty, branch, golden, yellow lotus and yellow white at a time. At this time, the mixing ratio of the herbal medicine is Gosam: Dansam: Casualties: Branch: Golden: Huangyan: Yellow white 1.0-4.0: 20-40: 1.0-4.0: 1.0-4.0: 40-60: 5.0-15: 1.0-4.0 It is preferable to mix and extract to consist of.

In the present invention, the antimicrobial composition is a composition having an antimicrobial and antifungal effect in which the mixture of the herbal extracts is contained in an amount of 70 to 100% by weight based on the total weight of the antimicrobial composition. Preferably, it can mean a composition having excellent antimicrobial activity against Candida albicans, a fungus that causes vaginitis, and Gardnerella Vaginalis, a common bacterium, and the development and function of bacteria and fungi. Means a substance capable of inhibiting or inhibiting.

The antimicrobial composition according to the present invention is not particularly limited in use, and may be used, for example, in women's cleaning composition for preventing or treating vaginitis. In addition, the cleaning composition for women can be used for cleaning, pharmaceutical, cosmetics, wet wipes and the like.

In addition, the cleaning agent may be formulated in a spray type, liquid type, gel type, powder, solid soap, foam type, and the like, and the cosmetics may be arbitrarily selected in the formulation, but a lotion, lotion, It may be made of cream, essence, aerosol, powder, oil or mist.

In addition, the pharmaceutical preparations may be formulated as tablets, pills, powders, granules, capsules, etc. as a solid preparation for oral administration, and as a form for parenteral administration, creams, ointments, lotions, dressing solutions, sprays, It may be formulated with other coatings and the like.

In this case, the vaginitis is Candida vaginitis, the causative agent of Candida vaginitis may be Candida albicans, and also the bacterial vaginitis, the causative bacterium of bacterial vaginosis, Gardnerella vaginalis. It may be

When the antimicrobial composition according to the present invention is used in a female detergent composition, it is preferable that 1 wt% to 20 wt% of the total weight of the female detergent composition is included. If the content is less than 1% by weight, there is a problem in that it is difficult to expect a substantial antibacterial and antifungal effect, and when the content exceeds 20% by weight, there is a significant value in that it is difficult to manufacture a product suitable for the formulation.

In addition, in the present invention, in addition to the herbal extracts described above, the cosmetic female cleanser composition is a variety of components commonly used in external preparations for skin, such as water-soluble components and powder components, in a range that does not lower the effect of the present invention as necessary. , Oils, surfactants, moisturizers, viscosity modifiers, preservatives, antioxidants, fragrances, colorants and the like can be blended.

In the present invention, the female cleanser composition for cosmetics may further include at least one additive selected from the group consisting of oil, water, surfactants, humectants, thickeners, chelating agents, pigments, preservatives, and fragrances. have.

On the other hand, the present invention, the natural extract antimicrobial composition 0.1 to 20% by weight; 0.1 to 0.2 wt% surfactant; 0.01-0.02% by weight of alkali metal benzoate; And it can provide a spray type cleaning composition for the prevention or treatment of vaginitis including the remaining purified water.

And, the present invention, the natural extract antimicrobial composition 0.1 to 20% by weight; 5-15% by weight of dimethicone copolyol; 5-15% cyclomethicone; Urea 10-30 wt%; Glycerin 10-30% by weight; 0.01 to 0.03 weight percent of triethanolamine; 0.01 to 2 wt% preservative; And it can provide a gel detergent composition for the prevention or treatment of vaginitis including the remaining purified water. At this time, the gel may be a detergent such as soap, it may be applied in an integral form of the detergent composition as a gel formulation.

In addition, the present invention, the natural extract antimicrobial composition 0.1 to 20% by weight; Sodium lauryl sulfate 1-3 wt%; Urea 0.5-1.5 wt%; L-menthol 0.2-0.7 wt%; 0.6 to 1% by weight of polyoxyethylene nonylphenyl ether; Ethanol 10-20 wt%; EDTA 0.02-0.08 weight percent; 0.01 to 2 wt% preservative; And it can provide a liquid detergent composition for the prevention or treatment of vaginitis comprising the remaining purified water. In this case, the liquid form may be a detergent such as liquid soap, and may be applied in an integral form of the detergent composition as a liquid formulation.

In addition, the liquid formulation may be applied to a non-woven fabric or pulp in the form of a wet tissue.

In addition, the present invention, 0.1 to 20% by weight of the natural extract antimicrobial composition; 10 to 15 weight percent wax; Glycerin, fatty acid ester 5-10% by weight; 0.1 to 1 weight percent aluminum phosphate; 0.1 to 1% by weight of ferric phosphate; 10-30 weight of sorbitol; It may be a detergent such as a soap containing a whole amount of purified water.

In addition, the present invention can be used as a natural extracting antimicrobial composition, a pharmaceutical composition for antibacterial and antifungal, a cosmetic composition including antibacterial and antifungal function, a skin cleaner having antibacterial and antifungal action, and a disinfectant having antibacterial and antifungal action. It can provide a pharmaceutical, cosmetics, detergents, disinfectants, wipes, and the like, including the natural extract antimicrobial composition.

In addition, the present invention can be prepared by various known health functional foods by the conventionally known method by adding a preference food ingredient to the antimicrobial composition comprising the herbal extract, it can also be formulated in a general food formulation, the additive ingredient is a formulation In accordance with the present invention, those skilled in the art can appropriately select and blend.

The present invention, in contrast to the conventional technology, in the case of conventional yeast infection can cause liver toxicity, digestive disorders, tolerance to vaginitis causing bacteria, in the case of applying ointment when contact dermatitis due to skin irritation occurs However, the herbal extracts of red ginseng, red ginseng, casualties, branch, golden, rhubarb and yellow white components of the present invention are eco-friendly herbal extracts extracted from nature and do not have side effects and toxicity such as irritation to the mucous membrane of the skin in human application. Applicable without risk.

In addition, the present invention is very excellent in antimicrobial activity to reduce the lesions of candida vaginitis and bacterial vaginosis and prevent the factors that occur continuously to exert an excellent effect in the prevention and treatment of the vaginitis, and to produce the superiority of the effect When applied to cosmetics, pharmaceuticals, detergents, wet wipes, etc. having a variety of formulations comprising the process or the composition of the present invention, there is no convenience of processing the formulation and there is no deterioration in the application of the formulations and accordingly It can be commercialized to be applied and used. Therefore, it can be conveniently used in various formulations for the prevention or treatment of Candida vaginitis and bacterial vaginitis. That is, the present invention is expected to be able to exert an excellent effect in terms of functionality, product usage and commerciality as a cleaning agent for the prevention and treatment of vaginitis.

Figure 1 is a photograph of the antimicrobial activity against Candida albicans (Candida albicans) according to the composition of the present invention was measured by a plate medium diffusion method using a paper disk.
2 is a photograph of the antimicrobial activity of Gardnerella vaginalis according to the composition of the present invention measured by a plate medium diffusion method using a paper disk.
3 is a graph measuring the antimicrobial activity against Candida albicans by the composition of the present invention using the liquid medium sensitivity test method.
Figure 4 is a graph measuring the antimicrobial activity against Gardnerella vaginalis by the composition of the present invention using the liquid medium sensitivity test method.
5 is a graph measuring the survival rate of Candida abicans, Lactobacillus acidphilus by the composition of the present invention.
Figure 6 is a graph of the survival rate measurement of Gardnerella vaginalis, Lactobacillus acidophilus by the composition of the present invention.
Figure 7 is a view showing the cytotoxicity measurement results by the composition of the present invention.

Hereinafter, specific details for carrying out the invention will be described in detail including an embodiment so that those skilled in the art (hereinafter, referred to as “an expert”) belong to the technical field of the present invention. .

The present invention provides a natural extract antimicrobial composition having an antibacterial and antifungal effect composed of herbal extracts mixed with red ginseng, red ginseng, casualty, branch, golden, yellow lotus and yellow white.

Example 1 Preparation of Natural Extractive Antimicrobial Composition

10 g of red ginseng, 10 g of saengsaeng, 10 g of yellow ginseng, 10 g of yellow white, 40 g of red ginseng, 160 g of golden ginseng, respectively, and then immersed in 30% ethanol at 10 times the total weight of herbal medicine After filtration through a filter, a sterilization treatment was performed using a 0.45 μm filter, and the extracted solution was concentrated under reduced pressure and dried to obtain 8.9 g of the natural extractive antimicrobial composition of the present invention.

<Test Example 1: Determination of antimicrobial activity by disc-diffusion method>

The disk diffusion method was used to confirm the antimicrobial activity of the natural extractive antimicrobial composition prepared in Example 1.

 1. Evaluation of antimicrobial activity against Candida albicans

100 μl of pre-cultivated Candida albicans was plated on a plate medium for measurement of disk diffusion (Sabouraud Dextrose Agar. Difco.USA), and then the extract was sampled at a concentration of 5 mg / disc on a sterile paper disc. Was dropped and then placed on the badge. As a control for this, the antifungal drug itraconazol (Itraconazol) was deposited on a paper disk at a concentration of 100 µg / disk and placed on a medium. The plate medium was incubated at 28 ° C. for 48 hours, and then the size of the clean zone was measured. The results are shown in Table 1 below. As shown in Table 1 below, the antimicrobial composition of the present invention showed a strong growth inhibitory effect compared to the control itraconazole (antifungal agent, Patent 10-0592066).

 2. Evaluation of Antimicrobial Activity against Gardnerella Barnacles

100 μl of pre-cultured Gardnerella vaginalis was plated in Reinforced clostridial medium.Difco.USA and plated, and then the extract was added to a sterile paper disc at 5 mg / disc. Samples were instilled in concentration and placed on the medium. As a control for this, the antibiotic ampicillin (Ampicillin) was deposited on a paper disk at a concentration of 10 µg / disk and placed on a medium. The plate medium was incubated at 37 ° C. for 48 hours, and then the size of the clean zone was measured. The results are shown in Table 1 below. As shown in Table 1, the antimicrobial composition of the present invention showed a strong growth inhibitory effect.

 Growth low ring size (mm) Strain Mixed Extract (Antibacterial Composition) Control group Candida albicans 26 30 Gardnerella Trousers 29 31

<Test Example 2: Determination of minimum inhibitory concentration (MIC) of the natural extract antimicrobial composition using the liquid medium sensitivity test>

IC ₅₀ (half maximal Inhibitory) is a concentration required to inhibit the antimicrobial activity and the microorganism by 50% using the Liquid Microdilution Susceptibility Tests method to confirm the antimicrobial activity of the natural extract antimicrobial composition prepared in Example 1 Concentration) values were measured.

  1.Evaluation of antimicrobial activity against Candida albicans

Candida albicans cells were diluted with Sabouraud Dextrose medium to 2 × 10 ⁵ cells / ml, and then treated in 96-well by serial dilution. Then, incubated for 24 hours at 28 ℃ and using a Microplate reader (BIO-RAD) to determine the IC ₅₀ value by measuring the absorbance at 570nm wavelength, the results are shown in Table 2 below.

  2. Evaluation of Antimicrobial Activity against Gardnerella Barnacles

Cell numbers of Gardnerella vaginalis were diluted with Sabouraud Dextrose medium to 2 × 10 ⁵ cells / ml and treated by concentration in 96-well by serial dilution. Then, incubated for 24 hours at 37 ℃ and measured the absorbance at 570nm wavelength using a Microplate reader (BIO-RAD) to determine the IC ₅₀ value, the results are shown in Table 2 below.

Strain IC ₅₀ (mg / ml) value Candida albicans 29.4 Gardnerella vaginalis 19.1

<Test Example 3: Determination of the effect on the microorganism of the natural extract antimicrobial composition>

Test to determine the effect of the natural extracting antimicrobial composition prepared in Example 1 on Lactobacillus acidophilus, Candida albicans, Gardnerella vaginalis Was carried out.

It carried out by the same method as Experimental example 2. In this method, the growth of microorganisms can be confirmed by increasing the turbidity in the medium. Through this, the survival rate of the microorganisms for the natural extractive antimicrobial composition of the present invention was obtained. The results are shown in Tables 3 and 4. As a result, it can be seen that the natural extract antimicrobial composition of the present invention does not significantly affect Lactobacillus ashdophyllus, but inhibits the growth of Candida albicans and Gardnerella vaginalis.

Concentration (mg / ml) 5 10 20 50 100 Survival Rate of Lactobacillus Ashdophyllus (%) 100 100 89.7 86.8 85.9 Candida albicans
Survival rate (%) 43.01 12.39 1.64 0.83 0

Concentration (mg / ml) 10 20 30 40 50 100 Survival Rate of Lactobacillus Ashdophyllus (%) 100 89.7 89.8 88.6 86.8 85.9 Gardnerella Trousers
Survival rate (%) 77 39.6 10.3 1.1 0 0

Test Example 4: Cytotoxicity Test on Naturally Extracted Antimicrobial Compositions

Fibroblasts (Human Diploid Fibroblast) were inoculated at a concentration of 5105 cells / μl in 96 wellplate and inoculated at 100 μl, followed by incubation for 24 hours. After incubation, the medium was removed and 90 μl each in a well containing no serum medium. Put in. 10 μl of the sample diluted to 100,200,300,400,500,1000µg / µl each is treated in each well and incubated. After incubation for 24 hours, 20 μl of MTT reagent dissolved at 5 mg / ml was added using Phosphate Buffered Saline (PBS) and incubated for 4 hours. After removing all of the medium containing the MTT reagent and the sample, 100μl acid isopropanol (0.04N HCliniso-propanol) was added to each well and stirred for 30 minutes, and the absorbance was measured at 570 nm using an ELISA reader. 36 hours and 48 hours after the culture was measured in the same manner. Table 3 shows the results of measuring the cell viability of the fibroblasts at the concentrations of the natural extracting antimicrobial composition. As a result, it can be confirmed that the natural extract antimicrobial composition of the present invention is not cytotoxic to fibroblasts.

Concentration (μg / ml) 100 200 300 400 500 1000 Cellviability
(% of control) 24 hours later 101.3 99.4 100.3 99 100.8 99.2 After 36 hours 101.2 101.7 99.8 100.2 98.5 98.2 48 hours later 101.3 101.6 101.1 99.8 99.2 98.4

<Example 2. Preparation of female detergent composition of aerosol spray formulation for the prevention or treatment of vaginitis>

An aerosol spray was prepared by the following ingredients to prepare a female detergent composition for the prevention or treatment of vaginitis, including the natural extract antimicrobial composition of the present invention in an aerosol spray formulation.

<Compound Component Content (wt%)>

20% natural extract antifungal composition

0.15% surfactant

Alkali metal benzoate 0.015%

Purified water balance

Total 100

<Example 3. Preparation of cleaning agent composition for women for the prevention or treatment of vaginitis-Preparation of cream in gel-type formulation>

Cream was prepared by the following ingredients to prepare a female detergent composition for the treatment and prevention of vaginitis, including the natural extract antimicrobial composition of the present invention.

Component Content (wt%)

20% natural extract antifungal composition

Dimethicone Copolyol 10.0%

Cyclomethicone 10.0%

Urea 20.0%

Glycerin 20.0%

Triethanolamine 0.02%

Preservative 1.0%

Purified water balance

<Example 4. Preparation of cleaning agent composition for women to prevent or treat vaginitis-Preparation of wet tissue in liquid form>

In order to prepare a female detergent composition for the treatment and prevention of vaginitis, including the natural extract antimicrobial composition of the present invention, a liquid composition was prepared by the following ingredients.

Component Content (wt%)

10% natural extract antimicrobial composition

Sodium Lauryl Sulfate 2.0%

Urea 1.0%

L-menthol 0.5%

Polyoxyethylene Nonylphenyl Ether 0.8%

Ethanol 15%

EDTA 0.05%

Preservative 1%

Purified water balance

The liquid composition as described above was prepared and applied to a wet tissue fabric (nonwoven fabric).

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements of those skilled in the art that can be used as equivalents of the present invention as defined in the following claims are also provided. It belongs to the scope of the present invention.

Claims (12)

In the antimicrobial composition having an antibacterial and antifungal effect comprising a natural herbal extract, the natural herbal extract is based on the weight ratio Gosam 1.0 ~ 4.0: Salvia 20-40: Casualties 1.0 ~ 4.0: Branch 1.0 ~ 4.0: Golden 40 ~ 60: 5.0 to 15: Natural extracts composed of sulfur white 1.0 to 4.0, which are extracted by using an extraction solvent, and are extracted by using an extraction solvent. Composition.
In the antimicrobial composition having an antimicrobial and antifungal effect comprising a natural herbal extract, the natural herbal extract is extracted by the same method of each herbal medicine as follows by weight ratio based on ginseng extract 1.0 ~ 4.0: Salvia extract 20 ~ 40: casualty extract 1.0 ~ 4.0 : Branch extract 1.0 ~ 4.0: Golden extract 40 ~ 60: Rhubarb extract: 5.0 ~ 15: Antibacterial, characterized in that the composition consisting of 1.0 ~ 4.0 and contained 70 to 100% by weight based on the total weight of the antimicrobial composition And a natural extract antimicrobial composition having an antifungal effect.
According to claim 1 or claim 2, wherein the natural herbal extract is extracted with one or more solvents selected from the group consisting of purified water, ethyl acetate, butyl acetate, methyl alcohol, propanol, isopropyl alcohol and butyl alcohol Naturally extracted antimicrobial composition having antibacterial and antifungal effect.
According to claim 1 or 2, wherein the antimicrobial and antimicrobial effect of the antibacterial Candida albicans (Candida albicans) and the general bacteria Gardnerella Vaginalis antibacterial characterized in that it has an antimicrobial effect And a natural extract antimicrobial composition having an antifungal effect.
Women's cleaning composition comprising a natural extract antimicrobial composition having an antibacterial and antifungal effect of claim 1 or 2 as an active ingredient.
The method of claim 5, wherein the antimicrobial composition is contained in an amount of 1 to 20% by weight based on the total weight of the female detergent composition, wherein the female detergent composition is used for cleaning of detergents, cosmetics, or wet wipes for the prevention of Candida vaginitis and bacterial vaginitis. Female detergent composition, characterized in that the use.
According to claim 6, wherein the cleaning agent is a spray type, solid type, liquid type, gel type soap or foam type cleaning agent for women, characterized in that the cleaning agent.
The composition of claim 6, wherein the cosmetic composition is formulated as a lotion, lotion, cream, essence, powder, aerosol, oil or mist.
1) washing and drying step of washing and drying the red ginseng, sweet ginseng, casualty, branch, yellow, golden, and yellow lotus as herbal medicines;
2) an extraction step of obtaining an extract by refluxing for 1 to 24 hours at 10 to 80 ° C by immersing in an extraction solvent consisting of 5 to 10 parts by weight of 25 to 35% alcohols, based on 1 part by weight of each herbal ingredient;
3) The extract obtained from the ginseng extract 1.0 ~ 4.0: Salvia extract 20 ~ 40: casualty extract 1.0 ~ 4.0: branch extract 1.0 ~ 4.0: golden extract 40 ~ 60: rhubarb extract 5.0 ~ 15: sulfur white extract 1.0 ~ 4.0 weight ratio Mixing step of mixing;
4) centrifugation of the mixed extract of the mixing step to remove the insoluble material, and only take the supernatant and filtered under reduced pressure, concentrated step of concentrating at 10 ~ 80 ℃ using a reduced pressure concentrator; antibacterial and antifungal effect Method of producing a natural extract antimicrobial composition having a.
1) washing and drying step of washing and drying the red ginseng, sweet ginseng, casualty, branch, yellow, golden, and yellow lotus as herbal medicines;
2) mixing the washed and dried herbal medicines ginseng 1.0 ~ 4.0: Salvia ginseng 20 ~ 40: casualties 1.0 ~ 4.0: Branch 1.0 ~ 4.0: golden 40 ~ 60: rhubarb 5.0 ~ 15: sulfur white 1.0 ~ 4.0 mixing step;
3) Extract to obtain an extract by immersion in the extract solution consisting of 5 to 10 parts by weight of 25 to 35% concentration alcohols, reflux cooling at 10 to 80 ℃ for 1 to 24 parts by weight of the mixture of the herbal medicine step;
4) centrifugation of the extract after the mixing step to remove the insoluble material, only the supernatant, and then filtered under reduced pressure, concentrated step of concentrating at 10 ~ 80 ℃ using a vacuum concentrator; having an antimicrobial and antifungal effect Method for producing a natural extract antimicrobial composition.
The method according to claim 9 or 10, further comprising a powdering step of obtaining a dry powder by lyophilizing from -20 ° C to 30 ° C for 36 to 48 hours after the concentration step of 4). Method of producing a natural extract antimicrobial composition having an antibacterial and antifungal effect.
The method of claim 9 or 10, wherein the reflux cooling is performed for 4 to 8 hours, the alcohol is at least one solvent selected from the group consisting of ethyl acetate, butyl acetate, methyl alcohol, propanol, isopropyl alcohol and butyl alcohol Method of producing a natural extract antimicrobial composition having an antibacterial and antifungal effect, characterized in that extracted with.

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