KR20050005196A - Extraction method of effective component for preventing and treating vaginitis and cleanser composition containing extract obtained using the same extraction method - Google Patents

Abstract

PURPOSE: Provided are an extraction method of active ingredients for preventing and treating vaginitis from licorice root, Rhemaniae Radix, Paeonia L., Ligustici Rhizoma, Angelicae Sinensis Radix, Torilidis Fructus, Licii Radicis Cortex and Angelicae Dahuricae Radix. And provided is a cleanser composition containing, as an active ingredient, the extract obtained thereby. CONSTITUTION: The active ingredients for preventing and treating vaginitis are extracted from licorice root, Rhemaniae Radix, Paeonia L., Ligustici Rhizoma, Angelicae Sinensis Radix, Torilidis Fructus, Licii Radicis Cortex and Angelicae Dahuricae Radix with purified water as an extracting solvent. The cleanser composition having sterilizing effect on Trichomonas vaginalis, Candida albicans and Hemophilus vaginalis is characterized by containing 1-90 wt.% of the extract as an active ingredient, based on the total weight of the composition.

Description

Extraction method of effective component for preventing and treating vaginitis and cleanser composition containing extract obtained using the same extraction method}

The present invention relates to a method for extracting an active ingredient useful for preventing and treating vaginitis, and a detergent composition containing the extract extracted by using the same. In addition, the present invention relates to a method for extracting an active ingredient and a cleaning composition containing the same, which have good inhibitory effects on pulmonary symptoms, pruritus and specific odor, which are the main symptoms of vaginitis.

Women's vaginitis is a gynecological disease that most women have with cold and hypothalamus. It is a disease that is not severely developed but is not easily eradicated even if treated, and is recurring. Patients with vaginitis have vulvar pruritus with subarachnomia and severe pain when urinating, especially in the epithelium, urinary tract and perineum at the vaginal opening. The quality is rich in nutrients and deep in the human body, so the temperature and humidity are high. Therefore, it is easy to invade various strains including bacteria or fungi which are the main causes of vaginitis. In other words, the environment is basically suitable for living bacteria or fungi, many diseases are likely to occur.

The most common vaginitis among young women of childbearing potential is the infection of Candida vaginitis and Hemophilus vaginitis called Trichomonas vaginitis, Monilial or Fungal vaginitis. And herpes virus vaginitis. Before puberty or postmenopausal, the vaginal epithelium is thin, so it is more easily infected by various causative organisms such as gonococcus and non-specific bacteria.

Trichomonas vaginitis is one of the most common female vaginitis, which is infected by a bacterium called Trichomonas vaginalis, a flagellar parasite. Even healthy people can have bacteria in their vagina, bladder, men's urethra or semen. Schizophrenia is the main symptom. Nitrogen, burning pain, dyspareunia, bleeding, and vaginal erythema come along and sometimes complain of pain during urination. This yeast infection is caused by the complicated living environment of modern people, disturbance of sexual morality, towels of public baths, swimming pool, oral contraceptives, and the incidence and recurrence rate are increasing, and resistance to various drugs is increasing. It is ongoing. The basic treatment for Trichomonas vaginitis is to maintain normal intravaginal acidity of pH 4.5-5.0 to prevent Trichomonas protozoa from living. Recently, metronidazole or flagyl drugs are known to be effective.

Candida vaginitis, along with trichomoniasis vaginitis, is the most common vaginitis that most women experience at least once in their lifetime. Ten percent of non-pregnant women who complain of vaginal secretion, and about one third of pregnant women, are one of the major gynecological diseases infected with this vaginitis. The major symptoms are lobulation and local pruritus. Burning sensation, dyspareunia, edema, vulvar pain, etc. The clinical findings include vulva and vaginal erythema, congestion, epidermal detachment, vesicles, and pustules. The cause of the disease is Candida albicans, a type of fungus, mainly found in moist areas such as feces, oral cavity, vagina, and adhesions. It is infected by infected hands, towels, clothes, public baths, and instruments, and also during sexual intercourse. do. Treatment of candidiasis vaginitis has been reported to be a combination treatment of ketoconazole and mycostatin. Recently, myconazole nitrate and clotrimazole as imidazole preparations have been reported. ) Is used effectively.

Haemophilus vaginitis, usually transmitted through sexual intercourse and classified as a sexually transmitted disease, causes inflammation of the vagina by Hemophilus vaginalis, the causative agent of nonspecific bacterial vaginitis, or in conjunction with other infections. Infection with Haemophilus usually results in a slight pruritus or vaginal discharge with an unpleasant odor. Often the vaginal epithelium shows local evidence of infection or a slight creamy gray homogeneous discharge. The treatment of Haemophilus vaginitis is effective with teramycin or in oral administration, and even with sulfa cream for several weeks. In recent years, flagyl is known to be the most effective treatment.

There is also herpes virus vaginitis, which is infected by the herpes virus. This vaginitis is usually transmitted through oral sex and may cause cervical cancer, so care should be taken. Herpes viral vaginitis, like other vaginitis, has symptoms such as pruritus, abnormal cold and cramps, and is accompanied by headache, boredom, and fever symptoms systemically. This vaginitis can be relapsed at any time if the cause is given, so the underlying treatment is difficult, but it can only relieve symptoms. Treatment of herpes viral vaginitis requires the use of creams or medicines containing viramid, but is not yet satisfactory and usually unnecessary without symptoms (above, gynecology, Calvin books, 545-). 551 (1991) / Yeast Infection, Journal of the Korean Medical Association, 36 (2), 182-190 (1993).

In order to prevent such vaginitis, the topical cleaner used until recently is sulfonated fatty acids or distilled lauryl alcohol, and is a transparent compound synthesized as an emollient, thickener, nutrient, foaming agent and organic disinfectant. Or lotion type has been shipped, but their shortcomings are that keratin is eliminated by lipolysis and corrosion by free alkali and free lactic acid, so that the skin feels dry and moisturized, and capillary circulation function decreases and Induces pruritus (H Rose One Green, K Crescentcent, D Lactataside-S).

In addition, as a general treatment for vaginitis, metronidazole and clindamycin are used for anaerobic bacterial infections, azole drugs are used for candida vaginitis, and estrogen cream is used for atrophic vaginitis. It was. However, the use of such a general yeast infection treatment or antibiotics can easily replace the normal lactic acid bacterium in the vagina, which maintains the proper acidity of secretion in the vagina, with pathogenic anaerobic bacteria. As it is difficult to cure, there are many side effects and recurrence is common.

On the other hand, to compensate for the above-mentioned defects of the yeast infection prevention and treatment, sanitary napkin coating composition (Korean Patent Publication No. 2001-0014909) and sanitary napkin pad (Korean Patent Publication No. 2002-0062474) prepared by using the herbal medicine, agar A detergent for cleaning local use (Korean Patent Registration No. 1979-0001672) is disclosed. However, the active ingredient for preventing and treating vaginitis using the herbal medicine disclosed in these documents has a problem that its efficacy is low and its value as a product is low.

Therefore, the technical problem to be achieved by the present invention is to solve the above problems, showing excellent bactericidal power against the vaginitis causative bacteria from the herbal medicine mixture applied to Palmultang of the drug preparation, as well as the inhibitory effect of pulmonary symptoms, pruritus and peculiar symptoms of vaginitis The present invention provides a method for extracting an effective ingredient which can be used very effectively for the prevention and treatment of vaginitis, and a detergent composition containing the same.

In order to achieve the above technical problem, the present invention comprises an extract of the herbal composition for preventing and treating vaginitis comprising the extract of the herbal medicine mixture consisting of licorice, turmeric, peony, cheonggung, donkey, casualty, phalanges and white paper as an active ingredient. to provide.

At this time, in the cleaning composition for preventing and treating vaginitis of the present invention, the mixed amount of licorice, turmeric, peony, cheonggung, donkey, casualty, phalanges and white paper are each composed of 1 to 30% by weight based on the total weight of the herbal mixture Based on the total weight of the herbal mixture, it is preferable to extract using 1 to 50 times purified water. On the other hand, the extract content of the herbal medicine mixture is preferably 1 to 90% by weight based on the total weight of the detergent composition. In particular, the detergent composition is more preferably made of any one formulation selected from the group consisting of lotion, essence, gel and lotion.

In addition, the present invention comprises the steps of (a) preparing a herbal mixture consisting of licorice, jihwang, peony, cheonggung, donkey, casualty, phalanges and white paper; (b) adding purified water to the prepared herbal mixture and heating to extract the active ingredient; And (c) filtering the extract containing the active ingredient; provides a method for extracting the cleaning agent active ingredient for preventing and treating vaginitis.

In the extraction method of the cleaning agent active ingredient for preventing and treating vaginitis of the present invention, it is preferable that the herbal medicine mixture is mixed with licorice, yogurt, peony, cheonggung, donkey, casualty, phalanges and white paper at a ratio of 1 to 30% by weight, respectively. , Purified water is preferably added at a ratio of 1 to 50 times the total weight of the herbal mixture.

Hereinafter, a method for extracting an active ingredient which can be used very effectively for the prevention and treatment of vaginitis according to the present invention and a cleaning composition containing the same will be described in detail.

Raw material herbal extracts of the extract contained as an active ingredient in the cleaning composition for preventing and treating vaginitis of the present invention can be easily purchased on the market, and the pharmacological effects of these materials introduced in the literature are as follows.

Licorice is the root of the Fabaceae perennial licorice (Glycyrrhiza glabra L.). According to the Korean Pharmacopoeia and the traditional Chinese medicine, licorice is a basic herbal material, which strengthens blood vessels in our body and acts as an anti-inflammatory, detoxifying, antibacterial and sedative agent. It is the most widely used herbal medicine to say that the drug's licorice, in harmony with all medicines to increase the efficacy as well as neutralize the poison.

It is the root of the Scrophulariaceae perennial grass (Rehmannia glutinosa L.), which is drunk and steamed several times. In Dongbogam, nourish and nourish the blood, add blood, add kidneys, black hair and energize. It contains iron, which is hematopoietic and clears blood. It also has anti-fungal action, antifungal action, astringent action and detoxification, and it is applied to the treatment of functional uterine bleeding.

Peony is the root of the Peony peony (Ranuculaceae) perennial herb (Paeonia lactiflora Pall.). The flowers are white, thick, and broken, with white flowers (gold peony), red or branched flowers, thin roots, and stones with tendons. It is used for the treatment of dysmenorrhea, dysmenorrhea, cold and cramps, dysmenorrhea and abdominal pain in embryos and diarrhea. It has anti-inflammatory effect because it regulates immune function, and sterilizes and suppresses pathogenic bacteria.

The uterus is the root of the Umbelliferae perennial Cnidium officinale M. It is also called gungung, and is used as a blood donor, tonic, and sedative in anemia, cold, dysmenorrhea, and gynecological diseases.

Angelica is the root of the Angelica acutiloba K. perennial (Umbelliferae). Angelica is famous for its rejuvenation potion. In motion therapy, it is used for anemia and dysmenorrhea as sedatives and tonics, like uterus, and it has a bidirectional control effect on the uterus to strengthen or relax the muscle contraction of the uterus. It is an important medicine for postpartum recovery, blood, and blood, and has excellent effects on other gynecological diseases. And inhibits E. coli, Dipus bacteria and the like.

The casualties are the fruit of the Umbelliferae perennial Cnidium monnieri C. or the Torilis japonica D. It is called pajacho, and it is used for washing the affected area with drug decoction when it is wet and itchy. In motion therapy, it has been used for a long time in genital diseases. Used to get rid of vaginitis and mucus secretions in women.

Phalanx is the root bark of Lycium chinense M., Solanaceae. Fruit is called goji berries. It is reported that the fruit is used as a medicine to treat back pain, asthenia, and small body.

White paper is also the root of the Angelica dahurica B. et H. of the Umbelliferae perennial plant. In motion therapy, it is used for sedatives, vaginal drops, and blood drops. It is used for skin ulcers due to anti-inflammatory and drainage effects. / Herbal Medicines Standards Collection, Korea Drug Export and Import Association, Korea Drug Research Institute, 2000).

The present inventors have conducted experiments on Trichomonas vaginalis, a fungus causing bacterium, Candida albicans and Hemophilus vaginalis, and the components extracted by mixing the above-mentioned herbal medicines have no side effects. In addition to showing a strong bactericidal power, it was found that the main symptoms of vaginitis are suppressing effects of sputum, pruritus and specific odor. Therefore, according to the present invention, when the extract of the above-described herbal medicine mixture is added to a cleaning composition such as a lotion, an essence, a gel, etc., it may be usefully used to effectively prevent or treat vaginitis without any side effects.

Detergent active ingredients useful for the prevention and treatment of vaginitis can be obtained, for example, by the following extraction method.

First, a herbal medicine mixture is prepared by mixing licorice, yohwang, peony, cheonggung, donkey, casualty, phalanges and white paper herbal medicine. The mixing amount of each herbal medicine is preferably 1 to 30% by weight based on the total weight of the total mixture.

Then, purified water is added to the prepared herbal mixture and heated to extract the active ingredient. Purified water is preferably added 1 to 50 times the total weight of the herbal mixture, the desired active ingredient is sufficiently extracted when heated from 60 to 100 ℃ for 4 hours while preventing purified water from evaporating.

Since the extract containing the effective ingredient obtained by filtration is very effective for the prevention and treatment of vaginitis, it can be added to the cleaning composition of various formulations such as lotion, essence, gel, lotion and the like. The pH of the cleaning composition is preferably adjusted to be 4.0 to 5.0.

The extract of the herbal medicine mixture, which is the detergent active ingredient, is preferably added in an amount of 1 to 90% by weight based on the total weight of the detergent composition. If the content of the extract is less than 1% by weight based on the total weight of the detergent composition, the bactericidal effect of the causative agent of the desired vaginitis may not be sufficiently achieved, and if the content exceeds 90% by weight, the stability of the detergent may be a problem. This may cause the appearance of the cleaning agent or may not be able to express a sufficient cleaning effect when used.

Hereinafter, the present invention will be described in detail with reference to Examples. However, embodiments according to the present invention may be modified in many different forms, and the scope of the present invention should not be construed as being limited to the embodiments described below. The embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art.

Extraction Example 1

50 g of selected licorice, 50g of sulfur, 50g of peony, 50g of Cheongung, Angelica 50g, 50g of casualty, 50g of phalanx and 50g of white paper were weighed into an extractor, and 8kg of purified water was added as an extraction solvent and extracted for 16 hours at 85 ° C. Filtration was performed to obtain a detergent active ingredient.

Example 1

The detergent of the cosmetic preparation containing the active ingredient of the detergent according to the extraction example 1 was prepared as follows according to the ingredients and contents shown in Table 1 below. At this time, the unit of component content is weight%. The component which melt | dissolved the components 4-5 in the components 2 and 3 was added and stirred to the component 1 which cooled the extracted active ingredient in the extraction example 1 at 75 degreeC. Subsequently, component 6 was added to adjust pH to 4.5, and component 7 was added and mixed.

Comparative Example 1

Except that the active ingredient of the cleaning agent according to the extraction example 1 was prepared in the same components and contents according to Example 1.

Ingredient Number ingredient Example 1 Comparative Example 1 One Active ingredient according to extraction example 1 84.0 - 2 Butylene Glycol 2.0 2.0 3 glycerin 2.0 2.0 4 Polysorbate 80 0.2 0.2 5 Incense, preservative Quantity Quantity 6 pH regulator Quantity Quantity 7 Purified water Remaining amount Remaining amount

Extraction Example 2

Selected licorice 40g, turmeric 30g, peony 20g, cheongung 40g, Angelica 40g, casualty 60g, phalanx skin 30g and white paper 40g each weighed into an extractor, added 4kg of purified water as an extraction solvent and extracted 20 hours at 95 ℃, 200 mesh filter cloth Filtration was performed to obtain a detergent active ingredient.

Example 2

The detergent of the essence formulation containing the active ingredient of the detergent according to Extraction Example 2 was prepared according to the ingredients and contents shown in Table 2 below. At this time, the unit of component content is weight%. The component which melt | dissolved the components 4-5 in the component 2 and 3 was added to component 1 which cooled the active ingredient extracted from the extraction example 2 at 75 degreeC, and stirred. Components 6 and 7 were then added and dispersed. Component 8 was added thereto to adjust the pH to 4.3 and prepared by adding component 9 and mixing.

number ingredient Example 2 One Active ingredient according to extraction example 2 43.0 2 Butylene Glycol 3.0 3 Fiji-400 2.0 4 Polyoxyethylene Cured Castor Oil 0.1 5 Incense, preservative Quantity 6 Carbomer 0.1 7 Hydroxyethyl Cellulose 0.5 8 pH regulator Quantity 9 Purified water Remaining amount

For the cleaning compositions prepared according to Examples 1, 2 and Comparative Example 1 described above, the bactericidal power against trichomonas protozoa, fungi Candida albicans and Haemophilus, which were the most common causative agents of vaginitis, was evaluated. Culture conditions for each strain are shown in Table 3 below. In other words, Trichomonas protozoa was cultured using TYM medium, Candida albicans Sabouraud Dextrose Broth and Agar, and Tryptic Soy Broth and Agar against Haemophilus.

Strain Strain number Culture condition badge Temperature Oxygen carbon dioxide Trichomonas - TYM (pH 6.2) 37 ℃ Anaerobic 5% Candida albicans ATCC 10231 Sabouraud DextroseBroth, Agar 28 ℃ Aerobic - Haemophilus ATCC 33384 Tryptic Soy Broth, Agar (pH 7.4) 35 ℃ Aerobic -

In Table 3, TYM medium (pH 6.2) was added to the primary distilled water by mixing the components 1 to 9 of Table 4, the volume was 900mL, and then the pH was adjusted using 1M KOH solution or HCl. Finally, component 9 was added and the total volume was made up to 1000 mL using distilled water, followed by filtration with a 0.22 μm filter.

Ingredient number ingredient content One Trypticase 20 g 2 Yeast extract 10 g 3 Maltose 5 g 4 L-cystein, Monohydrochloride 1 g 5 Ascorbic acid 1 g 6 Potassium Phosphate, Monobasic 1 g 7 Potassium Phosphate, Dibasic 1 g 8 Penicillin-Streptomycin 1 g 9 Horse serum 100 mL

Evaluation of bactericidal power against Trichomonas protozoa

Hanyang University Department of Parasitology 24 hours the trichomonas parasites received pre-sale from the classroom in TYM medium with the culture broth (about ⁵ x 10 5 cells / mL) into a 1mL in the medium such as a 4mL, in 37 ℃, 5% CO ₂ condition 2 0.12 mL, 0.3 mL, 0.6 mL, and 1.0 mL of the detergent of Example 1 were added to the concentrations (about 2.3%, 5.7%, 10.7%, 16.7%) to the culture solution stabilized by culturing for about an hour. Before adding the cleaning agent of Example 1 was 0 hours, after measuring the total number of viable cells at intervals of 0.5 hours, 1.5 hours, 24 hours after the cleaning agent is shown in Table 5 (unit of the measured number of viable cells is cells / mL ). On the other hand, the detergent of Comparative Example 1 was added at a concentration of 2.3% to measure the number of viable bacteria in the same manner.

Sample (concentration) 0 hours 0.5 hours 1.5 hours 24 hours Remarks Comparative Example 1 (2.3%) 100,000 110,000 110,000 400,000 increase Comparative Example 1 (10.7%) 100,000 120,000 130,000 200,000 Slightly increased Example 1 (2.3%) 100,000 120,000 80,000 1,100 decrease Example 1 (5.7%) 100,000 49,000 2,000 0 Death Example 1 (10.7%) 100,000 0 0 0 Death Example 1 (16.7%) 100,000 0 0 0 Death

Referring to Table 5, it can be seen that the cleaning agent of Example 1 has an excellent bactericidal effect against Trichomonas protozoa. In particular, when 5.7% or more of the cleaning agent of Example 1 was added, the charge gradually decreased and completely died.

In addition, in order to compare the bactericidal power against Trichomonas protozoa at Example 2, Comparative Example 1 and other companies' product concentrations of 2.3% and 10.7%, the same method as described above was performed. Table 7 shows.

Sample (concentration: 2.3%) 0 hours 0.5 hours 1.5 hours 24 hours Remarks Comparative Example 1 100,000 120,000 120,000 900,000 increase Example 2 100,000 120,000 50,000 1,000 decrease H company Rose one green 100,000 0 0 0 Death Company D lactaside-S 100,000 0 0 0 Death J Shams 100,000 120,000 160,000 120,000 calendar

Sample (concentration: 10.7%) 0 hours 0.5 hours 1.5 hours 24 hours Remarks Comparative Example 1 120,000 120,000 150,000 300,000 Slightly increased Example 2 120,000 0 0 0 Death J Shams 120,000 80,000 180,000 630,000 increase

In Table 6 and Table 7, the main components of Rose One Green of Company H are generally sodium lauryl sulfate for cleaning, sodium borate for antibacterial action, and sterilization and refreshing. It is composed of ethanol (Ethanol), D company lactaside-S is used for the purpose of antibacterial and sterilization Lactic Acid (Lactic Acid) and lactoserum (Lactoserumdried) to enhance the function of the lactic acid and nourish the tissue , J. Shams is a bactericide composed of Chlorhexidine Digluconate Solution, agar, red ginseng, leaf, mint and citric acid.

Referring to Tables 6 and 7, the cleaning agent of Example 2 shows the bactericidal activity against Trichomonas protozoa at a concentration of about 2.3% or more, and at 10.7% concentration, Roseone Green and D company Lacta using synthetic fungicides having many side effects. It showed bactericidal power comparable to seed-S. On the other hand, J company Shams, a product that does not use a synthetic fungicide, such as the cleaning agent of Example 2 can be seen that the germicidal power is very little or very weak.

Evaluation of bactericidal activity against Candida albicans fungi

Candida was incubated for 5 hours under the same conditions by adding 1 mL of Sabouraud Dextrose Broth, 50 mL, 28 ° C., 100 rpm, and 24 mL of culture solution to 49 mL of the same medium, and incubating the absorbance (660 nm) to a value near 1.0. 0.1 mL of the second passage culture was added to 4.9 mL of the same medium, and 0.1 mL and 0.25 mL of detergent were added to maintain a constant concentration (about 2.0% and 4.8%). Before adding the cleaning agent, 0 hours were taken, and 1 mL was taken at an interval of 0.5 hour and 1 hour, and diluted 1,000 times. After inoculating the diluted bacterial solution in a petri dish, the total viable cell count was measured by using the Pour-Plate method, in which the medium cooled to 45 to 50 ° C. was dispersed evenly, and the results are shown in Tables 8 and 9. The unit of viable cell count measured is cfu / mL.

Sample (concentration) 0 hours 0.5 hours 1 hours Remarks Comparative Example 1 (2.0%) 300 x 10 ³ 400 x 10 ³ 350 x 10 ³ calendar Example 1 (2.0%) 250 x 10 ³ 140 x 10 ³ 200 x 10 ³ calendar Example 1 (4.8%) 430 x 10 ³ 0 0 Death

Sample (concentration: 4.8%) 0 hours 0.5 hours 1 hours Remarks Comparative Example 1 450 x 10 ³ 570 x 10 ³ 500 x 10 ³ calendar Example 2 430 x 10 ³ 0 0 Death H company Rose one green 520 x 10 ³ 340 x 10 ³ 340 x 10 ³ calendar Company D lactaside-S 530 x 10 ³ 40 x 10 ³ 50 x 10 ³ decrease J Shams 580 x 10 ³ 260 x 10 ³ 260 x 10 ³ Slightly reduced

Referring to Table 8 and Table 9, as a result of adding 2.0% of the cleaning agent of Example 1, there was little charge variation, which is a result of the addition amount of the active ingredient is insignificant. Indeed, when 4.8% of the cleaning agents of Examples 1 and 2 were added, the carcasses were completely killed. Therefore, it was confirmed that the cleaning agents of Examples 1 and 2 had bactericidal power against the causative agent Candida albicans fungi of vaginitis.

On the other hand, H company Rose One Green and D company lactaside-S using a synthetic fungicide with a lot of side effects, J company Shams, which does not use a synthetic fungicide, such as the cleaning agent of the present invention even if there is no bactericidal power even at a concentration of 4.8% It is very weak.

Evaluation of bactericidal power against Haemophilus

Haemophilus bacteria were cultured for 15 hours under the same conditions by adding 0.1 mL of the bacterial solution incubated for 15 hours at Tryptic Soy Broth, 34 ° C, aerobic conditions. 0.12 mL of detergent was added to the cultured bacteria solution so as to have a constant concentration (about 2.3%). Before adding the detergent, 0 hours was taken, and 1 mL was taken at an interval of 0.5 hour and 1 hour, and diluted to 10,000 times. After inoculating the diluted bacterial solution in a petri dish, the total viable cell count was measured by using the Pour-Plate method, in which the medium cooled to 45 to 50 ° C. was dispersed evenly, and the results are shown in Table 10. The unit of viable cell count measured is cfu / mL.

Sample (concentration: 2.8%) 0 hours 0.5 hours 1 hours Remarks Comparative Example 1 300 x 10 ⁴ 150 x 10 ⁴ 210 x 10 ⁴ calendar Example 1 250 x 10 ⁴ 0 0 Death Example 2 250 x 10 ⁴ 0 0 Death H company Rose one green 250 x 10 ⁴ 0 0 Death Company D lactaside-S 250 x 10 ⁴ 0 0 Death J Shams 540 x 10 ⁴ 370 x 10 ⁴ 870 x 10 ⁴ No change

Referring to Table 10, the cleaning agents of Examples 1 and 2 were excellent in the bactericidal power against Hecophilus even at a concentration of about 2.3%, using Roseone Green Company H and Company D Lacta using synthetic fungicides having many side effects. It showed bactericidal power comparable to seed-S. On the other hand, it can be seen that the J company Shams, which does not use a synthetic fungicide like the cleaning agent of Example, does not have bactericidal power.

<Evaluation of Suppression, Pruritus and Specific Odor Inhibition>

In order to evaluate the inhibitory effect of the cleaning agent according to Example 2 on the major symptoms of vaginitis, pruritus, pruritus and peculiar odor, 20 volunteers regardless of age were tested. The women who agreed to this efficacy test usually had a schizophrenia, complained of pruritus and had a peculiar smell, but did not have any unusual physical abnormalities. Once a day, 50mL was diluted in 1L or stock solution was used for 4 weeks. We evaluated the inhibitory effect on the schizophrenia, pruritus and peculiar odor and the satisfaction of usability (cleanliness, refreshing feeling, etc.). Inhibition (3 points), 30% inhibition (2 points), no effect (1 point), and usability were also evaluated by satisfaction instead of the inhibitory effect by the 5-point method. The results of the evaluators are shown in Tables 11 and 12.

number Hypothyroidism suppression Suppressing pruritus Smell Suppression Usability age One 5 3 5 4 20's 2 5 5 5 5 20's 3 4 4 5 5 20's 4 5 5 5 5 30 spaces 5 4 5 5 4 30 spaces 6 4 4 4 4 30 spaces 7 5 5 5 5 30 spaces 8 5 4 4 4 30 spaces 9 3 3 3 3 30 spaces 10 4 5 2 3 30 spaces 11 2 4 4 4 40 spaces 12 3 4 4 3 40 spaces 13 4 5 5 5 40 spaces 14 5 4 5 5 40 spaces 15 4 4 4 4 40 spaces 16 3 3 4 3 40 spaces 17 4 3 4 5 40 spaces 18 4 3 5 5 40 spaces 19 4 5 4 4 50 spaces 20 4 4 4 4 50 spaces

Referring to Table 11, the average score of the evaluator for the evaluation items was 4.05 points for hyperhidrosis suppression, 4.10 points for suppressing pruritus feeling, 4.30 points for suppressing peculiar odor, and 4.20 points for usability. In addition, all 20 evaluators did not report any unusual side effects during the evaluation.

Hypothyroidism suppression Suppressing pruritus Smell Suppression Usability 5 points 6 7 9 8 4 points 10 8 9 8 3 points 3 5 One 4 2 points One 0 One 0

Referring to Table 12, only two patients were evaluated as having a weak inhibitory effect, and no evaluator evaluated as no effect.

As described above, most of the evaluators evaluated the inhibitory effect of 50% or more on the major symptoms of vaginitis, such as hypothalamus, pruritus and peculiar odor, and on average, it was evaluated to have an inhibitory effect of 70% or more. . In addition, all of the evaluators evaluated that the use of cleanliness, refreshing feeling, etc., after use is 50% or more, indicating that the inhibitory effect on schizophrenia, pruritus and peculiar odor is excellent.

As described above, the detergent containing the extract extracted from the herbal mixture according to the present invention has a superior bactericidal effect against trichomonas protozoans, the fungi Candida albicans and Haemophilus, which are the main causative agents of vaginitis, and is the main symptom of vaginitis, pruritus and pruritus. And the specific odor inhibitory effect is excellent. In addition, since it is excellent in cleanliness and refreshing feeling after use, it is expected to be able to exert an excellent effect in terms of functionality, commercialization and commercialization of the product for the prevention and treatment of vaginitis.

Claims (7)

Cleansing composition for preventing and treating vaginitis, characterized in that it contains as an active ingredient the extract of the herbal medicine mixture consisting of licorice, turmeric, peony, cheonggung, donkey, casualty, phalanges and white paper. According to claim 1, The extract of the herbal medicine mixture is composed of 1 to 30% by weight, respectively, based on the total weight of the herbal medicine mixture mixed with licorice, turmeric, peony, cheonggung, donkey, casualty, phalanges and white paper, the herbal medicine Cleanser composition for preventing and treating vaginitis, characterized in that extracted using 1 to 50 times purified water based on the total weight of the mixture. According to claim 1, wherein the extract content of the herbal mixture is a vaginitis prevention and treatment detergent composition, characterized in that 1 to 90% by weight based on the total weight of the detergent composition. According to claim 1, wherein the detergent composition is a detergent composition for preventing and treating vaginitis, characterized in that consisting of any one selected from the group consisting of lotion, essence, gel and lotion. (a) preparing a herbal mixture consisting of licorice, yogurt, peony, horoscope, donkey, casualty, phalanges and white paper; (b) adding purified water to the prepared herbal mixture and heating to extract the active ingredient; And (c) filtering the extract containing the active ingredient; characterized in that it comprises a method for extracting the active ingredient cleaning agent for preventing and treating vaginitis. The method of claim 5, wherein the herbal medicine mixture licorice, turmeric, peony, cheonggung, Angelica, casualties, phalanges and white paper, respectively, 1 to 30% by weight of the extraction of cleaning agents effective ingredient for preventing and treating vaginitis Way. [6] The method of claim 5, wherein the amount of purified water added in step (b) is 1 to 50 times the total weight of the herbal mixture.