KR101438395B1 - Antibacterial Composition for Moisturing Vagina Containing Natural Extracts - Google Patents

Abstract

The present invention relates to an antibacterial composition comprising natural extracts for moisturizing vagina and, more specifically, to an antibacterial composition comprising peony, licorice, cnidium, poncirus, pueraria, Angelica dahurica root, Sinomenium acutum, and Coryopteris divaricate extracts as active ingredients for moisturizing vagina. The antibacterial composition for moisturizing vagina of the present invention does not cause skin irritation by using the natural extracts, smoothens or moisturizes the skin by protecting the skin from external stimuli, and has an excellent antibacterial activity against pathogenic microorganisms that cause diseases associated with vagina and vaginal odor of vaginal discharges or menstrual blood.

Description

[0001] The present invention relates to an antimicrobial composition for moisturizing vagin,

The present invention relates to an antimicrobial composition for vaginal hosiery comprising a natural extract, and more particularly to a vaginal moisturizing composition containing an extract of Coryopteris divaricata as an active ingredient, such as peony root, licorice root, And to an antimicrobial composition for vaginal moisturization comprising the antimicrobial composition.

Over the years, women have suffered from vaginal infections that cause itching, pain and general discomfort. In particular, women have experienced vaginal yeast infections caused by the microorganism Candida albicans . Common treatments for vaginal yeast infections include topical application of creams, suppositories, soft gelatine capsules, vaginal tablets and vaginal ointments. These treatments generally can be used in a range of treatments ranging from 1 to 7 days. More recently, oral administration of antifungal compositions such as Diflucan tablets has been used in patients for systemic treatment.

In many cases, vaginal yeast infections are not limited to vaginal sites only. In fact, the infection can be extended and can include the vulvar region, which causes not only the inside but also the external nuisance and itching. A variety of concurrent therapies, including creams, suppositories or ovules for internal insertion, and creams for external application, for externally applying to the vulva to alleviate itching, pain and discomfort of the vulva, Have been used to treat both internal and external.

Women who are suffering from pain and discomfort from vaginal yeast infections are eventually relieved during the course of the known methods, but they may not be aware of this mitigation for several days. Although the application of topical cream provides some relief during the course of treatment, this relaxation is not immediate or immediate. Infection continues to produce dirty chunks of secretions that increase discomfort from infection.

Therefore, in order to prevent or treat infection, chemically synthesized therapeutic agents such as triclosan, gluconate-chlorhexidine glucuronate, lactic acid, butoconazole, clotrimazole, Azole antifungal agents such as miconazole, terconazole, tioconazole, fluconazole, ketoconazole and itraconazole have been used. However, these drugs have serious side effects (Tumor), Vol. 20, No. 12, December, 1977). In addition, there is a problem with drug interaction (BY Min, one day vaginal suppository treatment of trichomonas vaginitis with 1 gm of ornidazole.

On the other hand, the amount and viscosity of vaginal fluid secreted from the vagina is reduced by estrogen reduction, excessive work stress, bacterial contamination, and physiological impurity, thereby causing pain in normal cases.

In order to solve such a problem, many studies have been made to develop an antimicrobial composition for vaginal moisturization derived from natural products which has antibacterial and moisturizing effects and has no side effects. Korean Patent No. 0584223 discloses an antimicrobial composition comprising an ethyl acetate fraction extracted from green tea and a vaginal detergent containing the same, and Korean Patent Publication No. 2006-0059947 discloses a method for producing a natural bactericidal cleaning liquid containing an extract Korean Patent No. 0193105 discloses a composition for skin moisturizing agent containing gosam, celery, white paprika, jade, lily, brow roe, peony, pine, rhizome and the like.

However, there has been no report that Coryopteris divaricata , a natural plant, exhibits strong activity against female genital tract disease-causing bacteria, nor is it used in the prevention and treatment of female genitourinary tract diseases.

Accordingly, the present inventors have made intensive efforts to solve the above-mentioned problems. As a result of intensive efforts, the present inventors have found that, as a result of intensive efforts, the present inventors have found that a herbaceous plant having a strong antibacterial activity against peanut, licorice, Coryopteris divaricata ) has excellent effects as an antimicrobial composition for vaginal hosiery in women, and the present invention has been completed.

The object of the present invention is to provide a method for preventing and / or alleviating a female genitourinary tract disease, which is hypoallergenic and has no problem in inducing skin diseases and has an excellent antimicrobial activity against a causative agent of genitourinary tract diseases such as vaginitis, itching, And to provide an antimicrobial composition for vaginal moisturizing.

In order to accomplish the above object, the present invention provides an antimicrobial composition for vaginal moisturization, which comprises an extract of Coryopteris divaricata as an active ingredient, peach , licorice, licorice, flax, crab , white,

In the present invention, the extract is characterized by being extracted with water, an organic solvent or a mixed solvent thereof.

In the present invention, the organic solvent is selected from the group consisting of butylene glycol, methanol, ethanol, butanol, hexane, ether, benzene, chloroform and ethyl acetate.

In the present invention, the antimicrobial composition for vaginal moisturizing is one selected from the group consisting of Nicandra physaloides (L.) Gaertn. , Abutilon theophrasti Medicus and Ve ronicastrum sibiricum var. U uccarini Hara . Or more of the plant extract of the present invention.

In the present invention, the antimicrobial composition for quality control is characterized by having an antibacterial activity against a microorganism selected from the group consisting of Escherichia coli , Candida albicans , and Staphylococcus aureus .

The antimicrobial composition for vaginal hosiery according to the present invention does not cause skin irritation by using a natural extract, protects the skin from external stimuli to soften and moisturize the skin, and causes pathogenic It has excellent antimicrobial activity against microorganisms.

Hereinafter, the present invention will be described more specifically.

In the present invention, when a plant having a strong antimicrobial activity against a female genitourinary system-causing microorganism is used together with peanut, licorice, licorice, zygomycin, .

In the present invention, 50 kinds of plants were extracted with water and organic solvent in order to find a new plant whose antibacterial activity was unknown, and the antibacterial activity of the extracted plant extract was examined. As a result, it was confirmed that Coryopteris divaricata extract has excellent antimicrobial activity against inducing bacteria causing female genitourinary system diseases such as vaginitis, itching and odor.

That is, in one embodiment of the present invention, Escherichia coli , Candida albicans , and Staphylococcus aureus , which cause female genitourinary diseases such as vaginitis, itching and odor, An antimicrobial activity test of the extract was carried out and it was confirmed that the fruit extract of Pueraria intracutane had an excellent antibacterial activity against Escherichia coli , Candida albicans and Staphylococcus aureus Respectively.

Accordingly, the present invention relates to an antimicrobial composition for vaginal moisturization, which contains, as an active ingredient, an extract of peony root, licorice root, celia root, persimmon root, pustule, bark, coryopteris divaricata and the like.

The above-mentioned peony, licorice, astragalus, flea, pustule, white and bug are known to have antibacterial, moisturizing, and soothing effects on the skin.

Coryopteris divaricata is a perennial herb that grows in the middle of the central part of Korea. It is about 1m tall. Leaves are 8 ~ 13cm long and 4 ~ 8cm wide and are ovate, opposite, pointed end, There is a characteristic.

The antimicrobial composition for vaginal hosiery according to the present invention preferably contains 5 to 20 parts by weight of each of the above-mentioned peony root, licorice root, cinnabar, zebrafish, pustule, blank, and repellent extract per 100 parts by weight of the nutritional grass extract.

The extract can be obtained by any method conventionally used in the art. Preferably, the extract can be obtained by dissolving Coryopteris divaricata in water, an organic solvent or a mixture thereof. It is preferable to extract them with a mixed solvent thereof.

The organic solvent may be any solvent which is capable of separating an active ingredient from peanut , licorice, licorice, papaver, pustule , white, coryopteris divaricata and is not toxic, Methanol, ethanol, butanol, hexane, ether, benzene, chloroform, ethyl acetate and the like.

When the extract of the present invention is used, the extraction solvent is preferably used in an amount of 150 to 300 parts by weight based on 10 parts by weight of the dried plant, but is not limited thereto.

In the present invention, the antimicrobial composition for vaginal moisturizing is one selected from the group consisting of Nicandra physaloides (L.) Gaertn. , Abutilon theophrasti Medicus and Ve ronicastrum sibiricum var. U uccarini Hara . Or more of the plant extract of the present invention.

The part of the plant is not particularly limited, but Nicandra physaloides (L.) Gaertn. Is a fruit, leaf, stem; Abutilon theophrasti Medicus is a leaf, flower, seed; It is preferable to use leaves and roots for the ronicastrum sibiricum var. Z uccarini Hara.

Since the extract of Daechonjang, Kwangjiae or Chrysanthemum morifolium also has antimicrobial activity, when it is used together with Coryopteris divaricata extract, it can exhibit a better quality cleansing effect due to the synergy effect.

The extract of Daechonjang, Zawar or Chrysanthemum can be prepared in the same manner as described above, and may be contained in an amount of 10 to 100 parts by weight based on 100 parts by weight of the Coryopteris divaricata extract.

The antimicrobial composition for vaginal hosiery of the present invention may further contain various components commonly used in cosmetics such as water-soluble components, powder components, oils, surfactants, Humectants, viscosity regulators, preservatives, antioxidants, perfumes, pigments, synthetic antimicrobial materials, skin protectants, and the like.

Protecting the skin means to keep the skin intact to prevent further damage and pain as well as softening the site of infection. Skin protectants may include allantoin, cocoa butter, dimethicone, kaolin, shark liver oil, vaseline, castor oil, zinc oxide, and others known in the art.

The antimicrobial composition for vaginal hosiery of the present invention may be in the form of an emulsion such as cream, lotion, ointment, powder, microcrystal, liposome, gel or liquid. The emulsion may comprise an oil-in-water or water-in-oil emulsion. The antimicrobial composition for vaginal hosiery of the present invention may also contain intravaginal dosage forms such as creams, ointments, gels, gelatine capsules, suppositories and the like.

When the formulation of the present invention is a paste, a cream or a gel, an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, .

When the formulation of the present invention is a solution or an emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid esters of sorbitan, and the like can be used.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar, tracant and the like may be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether Alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, ethoxylated glycerol fatty acid ester, and the like can be used.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation or a surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, washed or rinsed with water. As a specific example, the soap may be liquid soap, powdered soap, solid soap, oil soap, and the surfactant-containing cleansing formulation may be a cleansing foam, a cleansing water, a cleansing towel, a cleansing pack, May be a cleansing cream, a cleansing lotion, a cleansing water or a cleansing gel, but the scope of the formulation is not limited thereto.

In the present invention, the above-mentioned antimicrobial composition for vaginal moisturization is preferably used as an effective amount of a microorganism which induces female genitourinary diseases such as vaginitis, itching and odor, for example Escherichia coli , Proteus mirabilis , Candida albicans Candida albicans), rugi labor Pseudomonas (Pseudomonas aeruginosa), Klebsiella species (Klebsiella spp.), Providencia species (Providencia spp.), Staphylococcus species (Staphylococcus spp.), Streptococcus species (Streptococcus spp , Bacteroide spp ., Mobiluncus spp ., Trichomonas spp ., Fusobacterium spp ., Preferably Escherichia coli , Candida albicans , Staphylococcus aureus , and the like.

Hereinafter, the present invention will be described in more detail by way of examples. The following examples are intended to further illustrate the present invention, and the scope of the present invention is not limited by these examples.

[Example 1] Production of plant extract

After purchasing the dried peonies, licorice, licorice root, flax seeds, purlins, white puffs, banggi, bukwangpu, dauncheon, The extract obtained by soaking for 2 days was filtered twice, and the filtrate was concentrated with a concentrator to obtain each butylen glycolysis extract.

[Experimental Example 1] Antimicrobial effect of plant extracts

The antimicrobial activity was comparatively evaluated by the following tests on the Butyleneglycolicol extracts of the intranasal herb, Daeheonjang, the diaper and the watery cold prepared in Example 1 above.

Staphylococcus aureus (Staphylococcus aureus; KCTC 1916) were cultured 18-24 hours enrichment at 35 ~ 37 ℃ after inoculation in TSB (Tryptic soy broth), Escherichia coli (Escheria coli; KCTC 2593) was LB medium (Luria-Bertani media) ( Candida albicans; KCTC 7695) was inoculated into YM (Yeast Malt) and cultured at 24-26 ° C for 18-24 hours. The cells were cultured at 35-37 ° C for 18-24 hours.

The enrichment Staphylococcus aureus (Staphylococcus aureus; KCTC 1916), Escherichia coli (Escheria coli; KCTC 2593) and Candida albicans (Candida albicans; KCTC 7695) the enrichment after each vaccination in solid medium previously used to culture, the paper in the medium The disk (diameter 8 mm) was placed. 50 μl of the plant extract prepared in Example 1 was added to the paper disk at a concentration of 10 mg, 5 mg, 1 mg and 0.5 mg, followed by culturing for 24 hours at the same temperature as that of the above enrichment culture. Inhibition Zone) was measured, and the results are shown in Table 1. At this time, 10 占 퐇 of 70% ethanol was used as a control.

Evaluation sample Inhibition zone (mm) dose (mg / disc) 10 5 One 0.5 Nutritional grass extract S. aureus 33 27 25 22 E. coli 31 25 23 19 C. albicans 27 25 20 18 Daechun raw extract S. aureus 26 22 20 18 E. coli 25 21 19 18 C. albicans 34 30 25 23 Eagle Extract S. aureus 26 25 22 15 E. coli 27 24 22 16 C. albicans 35 29 25 22 Chrysanthemum extract S. aureus 25 23 17 15 E. coli 24 22 16 15 C. albicans 29 25 21 20 70% ethanol S. aureus 9 8 8 8 E. coli 10 8 8 8 C. albicans 9 8 8 8

As shown in Table 1, the nutritional herb extracts were found in Staphylococcus aureus ; KCTC 1916) and E. coli (Escheria coli ; KCTC 2593), but the antimicrobial activity of Candida albicans ( KCTC 7695) was relatively low.

On the other hand, Daechun raw weight chumul, Abutilon theophrasti extract and hair naengcho extract of Staphylococcus aureus (Staphylococcus aureus; KCTC 1916), and Escherichia coli; only have an antimicrobial activity against (Escheria coli KCTC 2593), these more Candida albicans (Candida albicans; KCTC 7695) were found to have higher antimicrobial activity.

Therefore, even when the above nutritional grass extract, Daechonryeum extract, Euglena extract or Echinochloa crus-galli extract alone is used, it exhibits excellent antimicrobial activity against pathogenic microorganisms causing diseases associated with vaginal discharge or menstrual blood, When combined with Daechonju seaweed extract, Euglena extract or Euglena herbacea extract, can exhibit more excellent antimicrobial activity due to the synergistic effect.

[Experimental Example 2] Skin safety test

The skin stability of the extract prepared in Example 1 was evaluated. Fifty test subjects were subjected to a patch test using Haye's Test Chamber. Those who have experienced psoriasis, eczema, or other skin allergies, or those taking pregnant women, breastfeeding or contraceptives, or antihistamines are excluded from the study.

After the test site was wiped with alcohol, 15 쨉 g of each of the above extracts was dropped in a chamber and fixed on the upper part of the test site. The patching time was 24 hours. After the patches were removed, the test area was marked with a marking pen. The test area was observed 24 hours after each test. The test area was evaluated according to the criterion (see Table 2) Table 3 shows the results.

Criteria Evaluation standard score Negative No stimulation 0 Doubtful or slight reaction and erythema Small stimulus One Erythema + Induration Light stimulus 2 Erythema + Induration + Vesicle Moderate irritation 3 Erythema + Induration + Bullae River stimulation 4

Evaluation sample Skin irritation (average score) Peony extract 0 Licorice extract 0 Cinnabar extract 0 Ginseng extract 0 Puerariae Radix extract 0 White paper extract 0 Bran extract 0 Nutritional grass extract 0 Daechun raw extract 0 Eagle Extract 0 Chrysanthemum extract 0

It can be seen from Table 3 that the extracts of Peony root, Licorice root, Root root, Garlic root, White chestnut, Bangui root, Nutritional grass, Daechiwon juice,

[Experimental Example 3] Evaluation of effectiveness of antimicrobial composition for vaginal moisturization

0.3: 0.3: 0.3: 0.3: 0.3: 0.3: 0.2: 2. The mixture of the extract prepared in Example 1 (Peony root: licorice root: The composition for vaginal hygroscopic use was prepared by allowing 100 women to use the antimicrobial composition for vaginal hygiene in which 80% by weight of an antioxidant, 2: 2: 2), 2.9% by weight of a viscosity controlling agent, 18% by weight of a surfactant and 0.1% The results are shown in Table 4, and the results are shown in Table 4. The results are shown in Table 4.

Survey contents very good satisfied usually dissatisfaction Sum Soothing effect of skin itching 51 30 15 4 100 Moisturizing effect of skin 58 24 13 5 100 About refreshing 61 27 10 2 100 Inhibitory effect of unpleasant smell 72 20 6 2 100

From Table 4, it can be seen that 80% or more of the skin irritation soothing effect, the skin moisturizing effect, the refreshing effect, and the suppression effect of the unpleasant smell are satisfactory or very satisfactory. Therefore, It can be used effectively as an antimicrobial composition for moisturizing.

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will appreciate that such specific embodiments are merely preferred embodiments and that the scope of the present invention is not limited thereto will be. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (5)

An antimicrobial composition for vaginal moisturization, comprising an extract of Coryopteris divaricata as an active ingredient.
The antimicrobial composition according to claim 1, wherein the extract is extracted with water, an organic solvent, or a mixed solvent thereof.
The antimicrobial composition according to claim 2, wherein the organic solvent is selected from the group consisting of butylene glycol, methanol, ethanol, butanol, hexane, ether, benzene, chloroform and ethyl acetate.
The antimicrobial composition according to claim 1, wherein the antimicrobial composition for vaginal moisturization is selected from the group consisting of Nicandra physaloides (L.) Gaertn. , Abutilon theophrasti Medicus and Ve ronicastrum sibiricum var. Zuccarini Hara An antimicrobial composition for vaginal moisturization, which further comprises at least one plant extract.
5. The antimicrobial composition for vaginal hosiery according to any one of claims 1 to 4, wherein Escherichia coli coli), Candida albicans (Candida wherein the antimicrobial composition has antibacterial activity against microorganisms selected from the group consisting of albicans and Staphylococcus aureus .