KR102575660B1 - External composition having antibacterial properties and feminine cleanser comprising the same - Google Patents

Abstract

It relates to a composition for external application capable of treating and repairing mucosal damage while having antibacterial activity against Candida, and a technique for a feminine cleanser containing the same. The composition for external use includes ginseng callus culture extract, concha tree callus culture extract, rice callus culture extract, green tea callus culture extract, plantain extract, thief's cane root extract, mugwort extract, angelica root extract, agarwood stem extract, calendula root extract , motherwort extract, and hollyhock root extract as active ingredients, and has antibacterial activity against candida.

Description

Feminine cleanser containing a composition for external application having antibacterial activity

The present invention relates to a composition for external application having antibacterial activity and a feminine cleanser containing the same, and more particularly, to a composition for external application capable of treating and repairing mucosal damage while having antibacterial activity against Candida, and a composition for women including the same It's about cleaning agents.

The vagina is the most moist and warm place in a woman's body as it connects the vulva and uterus. Therefore, it is a suitable environment for bacteria or fungi, which are the main causes of vaginitis, and there is a high possibility of diseases caused by them.

Under normal conditions, many lactic acid bacteria live inside the vagina, making the inside of the vagina slightly acidic to suppress the growth of pathogens or fungi. When the balance in the vagina is disturbed due to a change in hormone balance, opportunistic pathogens, which are normally suppressed in growth under normal conditions, proliferate in the vagina, resulting in vaginitis.

Bacterial vaginosis, Candida vaginitis, Trichomonas vaginitis, and atrophic vaginitis have been reported for vaginitis.

The most common disease is bacterial vaginosis, caused by Gardnerella vaginalis. Vaginitis is caused by an overgrowth of anaerobic bacteria, which existed in less than 1%, while the number of lactobacilli living in the vagina is reduced due to an imbalance in acidity in the vagina. Although the trigger factors that cause changes in the normal vaginal flora are not known, it is assumed that repeated alkalinization of the vagina due to frequent intercourse or vaginal washing plays a role. When the lactic acid bacteria that produce hydrogen peroxide, which should normally be present, are lost, regeneration of the normal vaginal flora becomes difficult, so bacterial vaginosis often recurs.

In addition, women with bacterial vaginosis have an increased frequency of serious sequelae such as pelvic inflammatory disease (PID), PID after miscarriage, postoperative cuff cellulitis, and abnormal pap smears. Risks include preterm labor, premature birth, chorioamnionitis, and endometritis after caesarean section.

Candida vaginitis, a common disease along with bacterial vaginosis, is a disease in which the fungus Candida albicans inhabits the moist genital area and causes inflammation. Vaginal candidal vaginitis is a common disease in which 45% of women experience two or more recurrences per year.

Candida (mainly Candida albicans), normally present in the oral cavity, intestines, and vagina of healthy people, is abnormal when female hormones are abnormal due to pregnancy or long-term oral contraceptive use, and immunity is extremely weakened due to colds, diabetes, or AIDS. Doing so will cause infection.

It can also be caused by taking corticosteroids used as a treatment for obesity, allergy to antibiotics, or arthritis. In the case of outbreaks caused by administration of antibiotics, antibiotics kill beneficial lactic acid bacteria in the vagina, and in this case, the possibility of recurrence is high. It can also occur if you wear a tight girdle or pants that do not allow ventilation, or if you do not wipe the vulva well after bathing and wear wet panties, or if you do not change your pads often during menstruation.

Only 50% of women living with Candida albicans in the vagina have symptoms. There is a difference between coming out. Characteristic symptoms include thick and white discharge, and the vulva is very sensitive, causing pain or sometimes swelling and inflammation. There are burning sensation, dyspareunia, edema, vulvar pain, etc. Clinical findings include erythema of the vulva and vagina, congestion, epidermal detachment, vesicles, pustules, etc.

In order to prevent such female diseases, detergents (vaginal cleansers) and vulvar cleansers are often used. A topical cleanser commonly used to prevent vaginitis is a clear or lotion made by sulfonating fatty acids or distilled lauryl alcohol and synthesizing emollients, thickeners, nutrients, foaming agents, organic fungicides, etc. lotion) type has been shipped.

In addition, there are surfactants with detergency and povidone-iodine as pharmaceuticals as main components. Povidone-iodine, a disinfectant produced by the chemical synthesis described above, is known to be effective in preventing and treating vaginal infections such as Candida vaginitis, Trichomonas vaginitis, and other vaginal infections. It is known that it can cause contact dermatitis, etc., and it can cause hypothyroidism by increasing the level of inorganic iodine in the vagina or total iodine in the blood.

As a conventional technique for solving the side effects caused by the use of a disinfectant as a medicine as described above, in the case of a healthy woman, the pH of the vagina is maintained at pH 3.2 by the secretion of lactic acid by beneficial microorganisms in the vagina, and at such a low acidity, most of the Based on the fact that harmful bacteria are suppressed or killed, a pH 3.5 buffer solution formulation with lactic acid as the main component and lactoserum extracted from milk has been proposed. It is an intravaginal titratable acidity maintainer that maintains the condition, and it is difficult to expect fundamental treatment and prevention.

Therefore, it is possible to maintain vaginal pH in a normal state, which can be effective in preventing diseases related to the vagina of women, and is a hypoallergenic and easy-to-use feminine cleanser or vulvar cleanser. There is a need to develop a feminine cleanser that can provide a healthy environment.

Korean Patent Registration No. 10-1651530 proposed a method for manufacturing a feminine cleanser using herbal extracts. However, in addition to the method for manufacturing a feminine cleanser using the herbal extract, there is a need to develop a feminine cleanser (also referred to as "inner perfume") that is effective in preventing diseases related to the female urinary tract and is hypoallergenic and easy to use. .

In addition, general feminine cleansers contain chemical ingredients that can irritate and damage the skin or cause allergies, so there is a harmful controversy. It is required to have a preventive effect. Korean Patent Publication No. 10-2005-0118819 discloses a detergent composition mixed with natural substances, but uses a synthetic surfactant, and the recovery effect of mucosal damage caused by vaginitis is also limited.

Korean Patent Publication No. 10-2005-0118819 Korean Registered Patent No. 10-165153

An object of the present invention is to solve the above problems, and a composition for external application containing a natural extract that has antibacterial activity against Candida and can restore or restore damaged mucous membranes and is effective for preventing or treating women's related diseases. It is a technical task to provide

In addition, to provide a feminine cleanser prepared by applying a composition for external application containing a natural extract that has antibacterial activity against Candida and can restore or restore damaged mucous membranes and is effective for preventing or treating women-related diseases. as a technical challenge.

In order to achieve the above object, the composition for external application having antibacterial activity according to an embodiment of the present invention has antibacterial activity against candida, and contains ginseng callus culture extract, ginseng callus culture extract, rice callus culture extract, green tea It contains callus culture extract, plantain extract, thief's cane root extract, mugwort extract, angelica root extract, agarwood stem extract, calendula root extract, motherwort extract, and hollyhock root extract as active ingredients.

As an embodiment, the composition for external application having antibacterial activity includes 6 to 10.5 parts by weight of ginseng callus culture extract, 6 to 10.5 parts by weight of ginseng callus culture extract, 6 to 10.5 parts by weight of rice callus culture extract, based on 100 parts by weight of the total. , green tea callus culture extract 6 to 10.5 parts by weight, plantain extract 6 to 10.5 parts by weight, cane root extract 6 to 10.5 parts by weight, mugwort extract 6 to 10.5 parts by weight, angelica root extract 6 to 10.5 parts by weight, agarwood stem 6 to 10.5 parts by weight of extract, 6 to 10.5 parts by weight of calendula root extract, 6 to 10.5 parts by weight of motherwort extract, and 6 to 10.5 parts by weight of hollyhock root extract.

As an example, the composition for external application having antibacterial activity may further include at least two or more extracts from among centella asiatica extract, green tea extract, aloe vera leaf extract, and pomegranate extract for moisturizing and skin regeneration.

According to one embodiment, the composition for external application having antibacterial activity against Candida can be used as a natural active ingredient of any one of gels, foams, lotions, essences, creams, mists, tissues, and ointments for external applications.

According to one embodiment, the composition for external application is a cosmetic composition for antibacterial activity, a cosmetic composition for skin moisturizing, a cosmetic composition for preventing skin wrinkles, a cosmetic composition for improving skin wrinkles, a cosmetic composition for skin whitening, a cosmetic composition for improving skin elasticity, and It can be applied as a natural active ingredient applied to any one of cosmetic compositions for improving skin tone.

Feminine cleanser according to an embodiment of the present invention is ginseng callus culture extract, pagoda callus culture extract, rice callus culture extract, green tea callus culture extract, plantain extract, thief's cane root extract, bulgaris mugwort extract, angelica root extract, aloes A composition for external application containing tree stem extract, calendula root extract, motherwort extract, and hollyhock root extract as active ingredients and having antibacterial activity against Candida.

As an example, the composition for external application having the antibacterial activity of the feminine cleanser includes 6 to 10.5 parts by weight of ginseng callus culture extract, 6 to 10.5 parts by weight of ginseng callus culture extract, 6 to 10.5 parts by weight of rice callus culture extract, based on 100 parts by weight of the body. 10.5 parts by weight, 6 to 10.5 parts by weight of green tea callus culture extract, 6 to 10.5 parts by weight of plantain extract, 6 to 10.5 parts by weight of cane root extract, 6 to 10.5 parts by weight of mugwort extract, 6 to 10.5 parts by weight of angelica root extract, A composition for external application comprising 6 to 10.5 parts by weight of agarwood stem extract, 6 to 10.5 parts by weight of calendula root extract, 6 to 10.5 parts by weight of motherwort extract, and 6 to 10.5 parts by weight of hollyhock root extract.

A feminine cleanser according to an embodiment includes 1 to 3% by weight of the composition for external application with antibacterial activity.

The feminine cleanser according to one embodiment further includes lactic acid, clophenesin, and 1,2-hexanediol in addition to the composition for external application with antibacterial activity.

The feminine cleanser according to an embodiment includes 1 to 3% by weight of the composition for external application with antibacterial activity, 0.2 to 0.6% by weight of lactic acid, 0.7 to 1.5% by weight of 1,2-hexanediol, and 8 to 15% by weight of a moisturizer. , 0.5 to 1.2% by weight of a thickener and 0.8 to 1.5% by weight of additives including a flavoring agent, an emulsifier, and a preservative, and an excess solvent.

According to the composition for a feminine cleanser containing a natural extract of the present invention, it contains a natural extract capable of restoring or repairing damaged vaginal mucosa while having antibacterial activity against candida causing vaginal-related diseases, including irritation during use. It can provide a much healthier environment. It also shows the effect of preventing or treating vaginal-related diseases.

Figure 1 is a photograph showing whether or not the results of antibacterial activity against Candida.

Hereinafter, a composition for external application having antibacterial activity and a feminine cleanser containing the composition according to an embodiment of the present invention will be described in detail.

Since the present invention can have various changes and various forms, specific embodiments will be described in detail in the text. However, it should be understood that this is not intended to limit the present invention to the specific disclosed form, and includes all modifications, equivalents, and substitutes included in the spirit and scope of the present invention. Terms such as first and second may be used to describe various components, but the components should not be limited by the terms. These terms are only used for the purpose of distinguishing one component from another.

Terms used in this application are only used to describe specific embodiments, and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly dictates otherwise. In this application, terms such as "comprise" or "having" are intended to designate that a combination of features, numbers, steps, and elements described in the specification exists, but one or more other features, numbers, steps, It should be understood that the presence or addition of components or combinations thereof is not precluded.

Meanwhile, unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by a person of ordinary skill in the art to which the present invention belongs. Terms such as those defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related art, and unless explicitly defined in the present application, they should not be interpreted in an ideal or excessively formal meaning. don't

Composition for external application having antibacterial activity

According to an embodiment of the present invention, the composition for external application having antibacterial activity containing a natural extract includes ginseng callus culture extract, pine tree callus culture extract, rice callus culture extract, green tea callus culture extract, plantain extract, cane root extract of thief , mugwort extract, angelica root extract, agarwood stem extract, calendula root extract, motherwort extract, hollyhock root extract.

The term "callus" of the present invention refers to an amorphous cell mass capable of dividing and proliferating by culturing all or some tissues of a plant under appropriate conditions. Although the callus has no differentiation function and is in a dedifferentiated state, if it is regularly transplanted and cultured, it can proliferate indefinitely as it is, and can regenerate to shoots, roots, embryos, or complete plants when cultured under certain conditions. As such, the medium for culturing callus must necessarily contain plant hormones such as auxin and cytokinin. In particular, when cultured in a liquid medium, it is easy to separate into single cells, and when the medium or cell density is adjusted, a cell population derived from a single cell ( clone), it is possible to produce a plant into which the trait of the gene is introduced by introducing a specific gene into the isolated single cell and culturing it.

The ginseng callus culture extract applied to the composition for external application having antibacterial activity of the present invention is an active ingredient extracted from Korean ginseng (Panax Ginseng Cameyer) through stem cell culture technology. And it is applied for anti-wrinkle and improvement effects through cell regeneration ability.

The sophora tree callus culture extract is applied for antioxidant and anti-inflammatory effects.

The rice callus culture extract has an anti-aging effect to prevent skin aging, and is applied for wrinkle prevention and skin whitening effect.

The green tea callus culture extract is applied for antibacterial, anti-inflammatory and antioxidant effects, and at the same time, it is applied for effects such as relieving skin trouble, moisturizing, softening skin, skin elasticity, skin regeneration, improving blood circulation and soothing sensitive skin.

The plantain extract contains ingredients such as ascorbic acid and flavonoid, and is applied to improve skin wrinkles, improve elasticity, and maintain moisture by controlling antibacterial and inflammation. In the present invention, overall symptoms such as wrinkles, elasticity, and moisturizing can be effectively improved by using plantain extract.

The thief's cane root extract is a perennial herbaceous plant belonging to the leguminous family, and contains matrine as an active ingredient, is also effective for skin suppuration and eczema, and is applied to relieve itching even when the wife's vulva is severely itchy. In the present invention, the cane root extract of the thief has an excellent antibacterial effect and also exhibits various skin improving effects such as skin stabilization, whitening, and wrinkle improvement, so it was applied to have a good effect on the skin while serving as a natural preservative.

The mugwort extract has excellent skin safety and usability and was applied for anti-inflammatory and moisturizing effects. As an example, the mugwort extract may include Artemisia argyi, Artemisia princeps, Artemisia montana, Artemisia capillaris, Artemisia annua, Artemisia vulgaris, and Artemisia vulgaris. Artemisia scoparia), mountain white mugwort (Artemisia sieversiana), dragon mugwort (Artemisia dracunculus), bitter mugwort (Artemisia absinthium), southern wood (Artemisia abrotanum) or alpine wormwood (Artemisia umbelliformis) extract, but is not limited thereto, preferably , It may be an extract of Artemisia annua or Artemisia vulgaris.

The angelica root extract was applied to obtain anticancer, angiogenesis, wound healing, tissue damage healing, and antibacterial effects.

The agarwood stem extract was applied to maintain skin vitality by acting as an antibacterial agent on the skin, relieving inflammation, helping blood circulation smoothly, and playing a conditioning role on the skin.

The calendula root extract has antifungal and bactericidal effects and at the same time plays a conditioning role on the skin and was applied for vital skin care.

The motherwort extract has an antibacterial effect, is effective in suppressing skin fungi such as eczema or skin itching, soothes inflammation, helps blood circulation smoothly, and acts as a conditioner to the skin. Applied to manage skin vitality .

The hollyhock root extract is applied to obtain the effect of inhibiting the production of various microorganisms and anti-inflammatory and antibacterial effects, and at the same time, it improves and prevents skin troubles such as moisturizing and skin trouble improving effects, cell proliferation effects and anti-inflammatory and anti-itching effects, thereby improving the skin barrier function. It was applied to obtain a strengthening effect.

As an embodiment, the ginseng callus culture extract, ginseng callus culture extract, rice callus culture extract, green tea callus culture extract, plantain extract, thief's cane root extract, mugwort extract, angelica root extract, agarwood stem extract, calendula root Extracts, motherwort extracts, and hollyhock flower root extracts may be used in the range of 6 to 10.5 parts by weight of each of the extracts in the composition for external application having the antibacterial activity.

Specifically, it contains 6 to 10.5 parts by weight of a ginseng callus culture extract, 6 to 10.5 parts by weight of a ginseng callus culture extract, and 6 to 10.5 parts by weight of a rice callus culture extract, based on 100 parts by weight of the entire composition for antimicrobial active external application , 6 to 10.5 parts by weight of green tea callus culture extract, 6 to 10.5 parts by weight of plantain extract, 6 to 10.5 parts by weight of cane root extract, 6 to 10.5 parts by weight of mugwort extract, 6 to 10.5 parts by weight of angelica root extract to 10.5 parts by weight, including 6 to 10.5 parts by weight of agarwood stem extract, 6 to 10.5 parts by weight of calendula root extract, 6 to 10.5 parts by weight of motherwort extract, and 6 to 10.5 parts by weight of hollyhock root extract, respectively. do.

As another example, the composition for external application having antibacterial activity may be used in the range of 6 to 10.5% by weight of each of the above-described extracts based on 100% by weight of the total.

A composition for external application having antibacterial activity according to another embodiment of the present invention includes ginseng callus culture extract, pine tree callus culture extract, rice callus culture extract, green tea callus culture extract, plantain extract, cane root extract, mugwort extract, angelica In addition to root extract, agarwood stem extract, calendula root extract, motherwort extract, and hollyhock root extract, at least two or more pomegranate extract, centella asiatica extract, green tea extract, and aloe vera leaf extract may be included for moisturizing and skin regeneration.

As an example, each of the pomegranate extract, centella asiatica extract, green tea extract, and aloe vera leaf extract is preferably applied within a range of 6 to 10.5 parts by weight similar to the application range of the extract applied to the composition for external application.

The pomegranate extract has a lot of vitamins and estrogen, so it removes active oxygen that promotes skin aging, removes freckles and spots, and is applied to give vitality to the skin and maintain balance.

The Centella Asiatica extract acts to regulate and promote collagen synthesis in the human body, strengthens the vestibule to promote blood circulation, and acts as a conditioner to the skin to provide energetic skin care.

The green tea extract was applied for antibacterial, anti-inflammatory and antioxidant effects, and at the same time, it was applied for skin trouble relief, moisturizing, skin softening, skin elasticity, skin regeneration, blood circulation improvement and sensitive skin soothing.

The aloe vera leaf extract was applied to obtain an effect of strengthening the skin barrier function by healing and regenerating the damaged skin from external stimuli.

The composition for external application containing the natural extract of the present invention having antibacterial activity can exhibit effective antibacterial, anti-inflammatory, and antioxidant effects against candida, and can improve and prevent irritation or damage to the skin caused by vaginal-related diseases, thereby forming a skin barrier. It has a function-enhancing effect.

The composition for external application having antibacterial activity containing the natural extract of the present invention as an active ingredient is for preventing or treating vaginal-related diseases while having an antibacterial effect against Candida. includes those that are vaginal dryness, vaginal mycosis, and cold sores. The disease associated with the vagina refers to an infection or inflammatory state of the vagina characterized by symptoms such as vaginal discharge, odor, burning sensation, itching, dyspareunia, and dysuria.

Vaginal dryness is a phenomenon in which vaginal secretion, which should normally come out, is reduced due to a lack of sexual secretion or a decrease in blood flow and blood circulation around the uterus and vagina. When female hormones decrease, epithelial cells of the vagina and urethra that lose elasticity and flexibility become thin. do. If vaginal dryness lasts for a long time, immunity can be lowered and exposed to various diseases, and the risk of urinary incontinence and uterine prolapse due to reduced contractility follows.

Vaginal mycosis is a vaginitis caused by the fungus Candida, which is one of the most common vaginitis that causes inflammation while inhabiting the wet genital area. The main symptom is a lot of cold and itching due to an inflammatory reaction on the skin. In addition, depending on the type and severity of the disease, secondary bacterial infection, etc., there are differences such as peeling of the skin or discharge of pus.

Cold soreness is a kind of circulatory disorder caused by poor blood circulation in the entire body or parts of the body, and is a symptom in which the amount of cold, a secretion secreted from the female genital organs, increases. The reason for this secretion is a natural phenomenon to protect the genital organs from various harmful bacteria, but when the amount of the secretion increases, it is heavily stained on underwear and the amount is large, leading to a damp and damp state, resulting in odor, and the most common cause of vaginitis. susceptible to exposure of Candida albicans.

That is, the composition for external application with antibacterial activity using a natural extract as an active ingredient has an antibacterial activity against Candida and can be used for preventing or treating vaginal-related diseases, and is not limited to the above examples. . The composition includes one having an antibacterial activity against Candida albicans, which is a fungus.

Feminine cleanser containing a composition for external application

Feminine cleansers having antibacterial activity against Candida according to an embodiment of the present invention include ginseng callus culture extract, pine tree callus culture extract, rice callus culture extract, green tea callus culture extract, plantain extract, cane root extract, mugwort extract, angelica root extract, agarwood stem extract, calendula root extract, motherwort extract, hollyhock flower root extract, and a composition for external application having antibacterial activity as an active ingredient.

According to an embodiment of the present invention, the composition for external application having antibacterial activity applied to a feminine cleanser having antibacterial activity against Candida is at least one of centella asiatica extract, green tea extract, aloe vera leaf extract, and pomegranate extract for moisturizing and skin regeneration. It may further include two or more extracts.

As an example, the feminine cleanser having antibacterial activity against Candida may include a composition for external application and lactic acid, clofenesin, 1,2-hexanediol, and the like.

Since the detailed description of the composition for external application having antibacterial activity included in the feminine cleanser has been described in detail above, it will be omitted to avoid redundancy.

A feminine cleanser according to an embodiment of the present invention contains 1 to 3% by weight of the composition for external application with antimicrobial activity, 0.2 to 0.6% by weight of lactic acid, 0.7 to 1.5% by weight of 1,2-hexanediol, and 8 to 15% by weight of a moisturizer. 0.5 to 1.2 wt% of a thickener, 0.8 to 1.5 wt% of additives including a flavoring agent, an emulsifier, and a preservative, and an extra solvent.

Lactic acid contained in the feminine cleanser is used to maintain the vaginal pH at a slightly acidic level. This is a component added to keep the vaginal pH slightly acidic.

The 1,2-hexanediol is used to improve skin conditioning and preservative properties of women's iron economy. As an example, butylene glycol may be used as the moisturizing agent, and carbomer may be used as the thickening agent.

Other additives may include pH adjusters, flavoring agents, emulsifiers, preservatives, sequestering agents, and the like. As an example, tromethamine as a pH adjusting agent, natural herb oil, ninalul as examples of flavoring agents, and PEG-40 Hydrogenated Castor Oil and PEG-60 Hydrogenated as examples of emulsifiers Castor oil etc. are mentioned.

The solvent may be used alone with distilled water or in combination with at least two of distilled water, alcohol, and glycol. Examples of the solvent include sterilized water, distilled water, ethanol, and butylene glycol.

In one embodiment, the feminine cleanser of the present invention can be applied as an external skin preparation in a liquid formulation such as a solution type, suspension type, emulsion type, etc. such as liquid, gel, cleanser, cream, ointment, dressing solution, spray, and other coating agents. can

Example 1

A composition for external application having antibacterial activity against Candida was prepared by mixing the natural extracts shown in Table 1 below.

raw material name Content (parts by weight) g Ginseng Callus Culture Extract 8.34 Pine tree callus culture extract 8.34 Rice callus culture extract 8.34 Green Tea Callus Culture Extract 8.34 plantain extract 8.33 Thieves Cane Root Extract 8.33 mugwort extract 8.33 Angelica Root Extract 8.33 Agarwood Stem Extract 8.33 calendula root extract 8.33 motherwort flower/leaf/stem extract 8.33 plate flower root extract 8.33 sum 100

Example 2 A feminine cleanser having antibacterial activity against Candida was prepared by mixing the natural extracts and additional substances shown in Table 2 below in the amounts shown in Table 2.

No. matter content One Composition for external application of Example 1 16g 2 Butylene Glycol 100g 3 ethanol 15g 4 1,2-Hexanediol 10g 5 carbomer 7g 6 Lactic Acid 4g 7 tromethamine 3g 8 clophenesin 2g 9 Disodium EDT 1g 10 PEG-40 Hydrogenated Castor Oil 5g 11 lavender oil 1g 12 linalool 0.3g 13 Purified water 835.7g

CFU/mL) 세균을 접종한 페트리접시(배지)를 각각 준비한다. 이후 아무 것도 처리도 안한 블랭크 배지(blank: A)와, 실시예 2의 여성 청결제 시료 희석액(여성 청결제 80중량%와 정제수 20중량%를 혼합 희석함)을 투입한 후 배지(B)를 각각 24시간동안 균주배양 하여 각각의 배지의 칸디다균 세균 농도를 측정하였다. 그 결과를 하기 표 3에 개시되어 있다. Test evaluation According to the KCL-FIR-1002 antibacterial activity test method, Candida (initial inoculation concentration)

CFU/mL) Prepare each petri dish (medium) inoculated with bacteria. Thereafter, a blank medium (blank: A) without any treatment and a dilution solution of the female cleanser sample of Example 2 (mixed and diluted with 80% by weight of the female cleanser and 20% by weight of purified water) were added, and then the medium (B) was 24 The strain was cultured for a period of time and the concentration of Candida bacteria in each medium was measured. The results are disclosed in Table 3 below.

As can be seen from the results of Table 3 and medium B of FIG. 1, Candida strains increased in blank medium (blank: A), whereas most Candida strains were found in medium (B) to which the sample dilution of Example 2 was added. confirmed to be extinct. That is, it was confirmed that the feminine cleanser comprising the composition for external application of the present invention has excellent antibacterial activity against Candida.

Claims (11)

delete delete delete delete delete 1 to 3% by weight of a composition for external application with antibacterial activity against Candida, 0.2 to 0.6% by weight of lactic acid, 0.7 to 1.5% by weight of 1,2-hexanediol, 8 to 15% by weight of a moisturizer, and 0.5 to 0.5% by weight of a thickening agent It is characterized by including 1.2% by weight and 0.8 to 1.5% by weight of additives including flavoring agents, emulsifiers and preservatives and an excess solvent,
The composition for external application with antibacterial activity against Candida is 6 to 10.5 parts by weight of ginseng callus culture extract, 6 to 10.5 parts by weight of ginseng callus culture extract, 6 to 10.5 parts by weight of rice callus culture extract, based on 100 parts by weight of the composition for external application with antimicrobial activity. 6 to 10.5 parts by weight of green tea callus culture extract, 6 to 10.5 parts by weight of plantain extract, 6 to 10.5 parts by weight of thief's cane root extract, 6 to 10.5 parts by weight of mugwort extract, 6 to 10.5 parts by weight of angelica root extract, agarwood 6 to 10.5 parts by weight of stem extract, 6 to 10.5 parts by weight of calendula root extract, 6 to 10.5 parts by weight of motherwort extract, and 6 to 10.5 parts by weight of hollyhock root extract. delete The method of claim 6, wherein the composition for external application having antibacterial activity further comprises at least two or more extracts from among centella extract, green tea extract, aloe vera leaf extract, and pomegranate extract for moisturizing and skin regeneration. A feminine cleanser with antibacterial activity.

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