WO2021177011A1 - キャピラリアレイユニット及び電気泳動装置 - Google Patents
キャピラリアレイユニット及び電気泳動装置 Download PDFInfo
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- WO2021177011A1 WO2021177011A1 PCT/JP2021/005526 JP2021005526W WO2021177011A1 WO 2021177011 A1 WO2021177011 A1 WO 2021177011A1 JP 2021005526 W JP2021005526 W JP 2021005526W WO 2021177011 A1 WO2021177011 A1 WO 2021177011A1
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- array unit
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44708—Cooling
Definitions
- the present invention relates to an electrophoresis apparatus, and particularly to an attachability of a capillary array unit and an air constant temperature bath of the electrophoresis apparatus.
- Capillary electrophoresis is widely used as a technique for separating and analyzing many biological samples including deoxyribonucleic acid (DNA).
- Capillary electrophoresis keeps the capillary filled with the electrophoresis medium at a constant temperature and applies a high voltage to separate the samples. Since the temperature of the migration medium affects the migration speed of the sample, it is important to keep the capillary at a constant temperature in order to obtain analytical performance. Variations in the wind speed in the constant temperature bath also cause variations in the temperature of the capillaries, which reduces analytical performance.
- Patent Document 1 discloses a removable wind direction control plate that forms a flow path for swirling the air discharged from the fan discharge port of the constant temperature bath according to the length and number of capillaries. Even if the length and number of capillaries change, the temperature of the entire capillaries can be adjusted at a uniform wind speed, and variations in the temperature of the capillaries can be reduced.
- the wind direction control plate is arranged curvedly along the outer circumference of the capillary. Therefore, when the capillary is attached / detached from the pump mechanism, the range of motion of the capillary is limited by the wind direction control plate, and it is difficult to attach / detach the capillary.
- an object of the present invention is to provide an electrophoresis apparatus that reduces temperature variation in a constant temperature bath and makes it easy to attach and detach capillaries.
- the electrophoresis apparatus of the present invention includes a capillary array unit having a capillary and a support for supporting the capillary, a pump mechanism in which one end of the capillary is connected to send a separation medium to the capillary, and a part of the capillary. It has a detection unit that irradiates light to measure the sample in the capillary and a constant temperature bath in which the capillary is housed and controls the temperature of the capillary.
- the constant temperature bath or capillary array unit is characterized by having a rectifying plate.
- the electrophoresis apparatus of the present invention can reduce temperature variation in a constant temperature bath and easily attach / detach capillaries.
- the figure which shows the basic structure of the electrophoresis apparatus The figure which shows the capillary array unit. The figure which shows the capillary array unit which the 2nd frame was restrained by the holding part. Diagram of separator.
- the figure which shows the attachment to the constant temperature bath of a load header The figure which shows the state after mounting the load header in a constant temperature bath.
- the figure which shows the state after mounting the capillary head on a block The figure which shows the detail of mounting the capillary head to a block.
- FIG. 1 is a schematic view showing the basic configuration of the electrophoresis apparatus 100.
- the electrophoresis apparatus 100 mainly includes a capillary array 102 including a capillary 101, an electrophoresis unit 104, a liquid feeding mechanism 106 that feeds a highly viscous polyma solution (hereinafter referred to as a polyma) as an electrophoresis medium to the capillary 101, and electrophoresis. It has an irradiation detection unit 108 that optically detects the sample separated by.
- the capillary array 102 includes one or a plurality of capillaries 101, a capillary head 110, and a load header 112.
- the capillary array 102 is replaced when it is used a predetermined number of times, when the analysis item is changed, or the like.
- the capillary 101 is a quartz pipe, and the outer cover of the capillary 101 is coated with a polyimide resin in order to improve the strength.
- the capillary head 110 is a member that is attached to and detached from the liquid feeding mechanism 106 in a pressure-resistant manner. In the capillary head 110, when there are a plurality of capillary 102, one end of the capillary 101 is bundled.
- the load header 112 is provided with a tubular cathode electrode 114.
- the capillary 101 is fixed to the load header 112 with the capillary cathode end 116 protruding from the lower end of the cathode electrode 114 so as to penetrate the cathode electrode 114.
- the capillary array 102 is attached and detached as a capillary array unit while being positioned on the frame. The configuration of the capillary array unit will be described later.
- the electrophoresis unit 104 has a constant temperature bath 118, a cathode side buffer container 122 for accommodating the buffer solution 120, and a high voltage power supply 124.
- the constant temperature bath 118 houses the capillary array 102 and regulates the temperature of the capillary array 102.
- a Perche element is used as the heat source of the constant temperature bath 118, and the temperature can be set from a temperature lower than room temperature to a high temperature of 50 ° C. or higher.
- the constant temperature bath 118 has a fan, and the air in the constant temperature bath 118 is circulated by the fan to reduce the temperature variation in the constant temperature bath.
- the capillary cathode end 116 and the cathode electrode 114 are immersed in the buffer solution 120 in the cathode side buffer container 122. In this state, a voltage is applied by the high voltage power supply 124.
- the liquid feeding mechanism 106 has a pump 126, a block 128 having a flow path inside, a polymer container 132 containing a polymer 130, and an anode-side buffer container 136 containing a buffer solution 134.
- the anode electrode 138 is immersed in the buffer solution 134 of the anode side buffer container 136.
- the block 128 is a connection portion for communicating the capillary head 110, the polymer container 132, and the anode side buffer container 136, and the polymer 130 in the polymer container 132 is sent to the capillary 101 by the pump 126 via the block 128.
- the buffer solution 134 in the anode-side buffer container 136 and the capillary 101 are electrically connected.
- the irradiation detection unit 108 has a light source 140 and a detector 142.
- the excitation light from the light source 140 is applied to the detection position 144 of the capillary 101, and the sample-dependent light is emitted from the sample passing through the detection position 144 of the capillary.
- the emitted light is detected by the detector 142.
- the detection position 144 of the capillary 101 has the polyimide resin coating removed so that the light emitted inside leaks to the outside.
- the vicinity of the detection position 144 is array-fixed to the optical flat plane with an accuracy of several microns in height by the detection unit 146 provided in the capillary array 102.
- the detection unit 146 is, for example, a substrate having a groove for aligning the capillary 101.
- an autosampler for transporting the sample container (not shown) and the cathode side buffer container 122 to the capillary cathode end 116 is provided.
- the autosampler connects the sample container to the capillary cathode end 116.
- the sample container has a large number of wells, and each well contains a solution containing a sample such as fluorescently labeled DNA.
- the capillary cathode end 116 is immersed in a solution containing the sample in the well.
- the sample is introduced into the capillary 101 from the capillary cathode end 116 by applying a voltage of about several kV between the anode electrode 138 and the cathode electrode 114 by the high voltage power supply 124.
- the capillary cathode end 116 is inserted into the cathode side buffer container 122 as shown in FIG.
- the sample is electrophoresed and separated in the capillary 101. The separated sample is detected by the irradiation detection unit 108.
- FIG. 2A is a rear view of the capillary array unit 200.
- the capillary array unit 200 is mainly a holding unit that detachably holds the capillary array 102, the first frame 202 that holds the capillary 101 in a curved line, the second frame 204 that holds the capillary in a straight line, and the second frame 204. It has a rectifying plate 210 for adjusting the direction of wind by a fan in 206, a separator 208, and a constant temperature bath 118.
- the first frame 202 is fixed to the load header 112 and is integrated with the capillary array 102.
- the second frame 204 supports the vicinity of the detection unit 146 from the capillary head 110 so as to prevent the capillary head 110 and the detection unit 146 from hanging down due to gravity.
- the first frame 202 has a guide 212 for moving the second frame 204 within a predetermined range.
- the second frame 204 can move in a straight line along the guide 212. The details of the mounting procedure of the capillary array unit will be described later, but by moving the second frame 204 in the direction of arrow A, the capillary head 110 and the liquid feeding mechanism 106 are connected, and the second frame 204 is moved in the direction of arrow B.
- the capillary head 110 has a hole or a groove, and the second frame 204 is provided with a convex portion that engages with the hole or the groove provided in the capillary head 110.
- the second frame 204 and the capillary head 110 can be prevented from coming off, and the capillary head 110 can be stably pulled out from the liquid feeding mechanism 106.
- the second frame 204 is restrained by the holding portion 206 provided in the first frame 202.
- a push latch is used as the holding portion 206.
- the second frame 204 which is not fixed to the holding portion 206, is moved in the direction of the arrow B and pushed into the holding portion 206, so that the second frame 204 is restrained by the holding portion 206. Further, the second frame constrained by the holding portion 206 is moved in the direction of the arrow B and pushed into the holding portion 206 again to release the restraint by the holding portion 206.
- FIG. 2A shows a state of the capillary array unit when the capillary head 110 is connected to the liquid feeding mechanism 106.
- a heat radiating sheet 214 (215) for enhancing the heat radiating performance of the capillary 101 is provided between the second frame 204 and the capillary 101.
- the heat radiating sheet 214 (215) By bringing the heat radiating sheet 214 (215) into contact with the capillary 101, the heat generated from the capillary 101 when a high voltage is applied to the capillary 101 can be efficiently dissipated, and the analysis performance can be improved.
- Two heat radiating sheets 214 (215) are provided between the capillary head 110 of the second frame 204 and the detection unit 146, and on the upper side of the detection unit 146.
- a fixture 216 for arranging the capillary 101 on the heat radiating sheet 214 (215) is connected above the heat radiating sheet 215 of the second frame 204.
- the fixture 146 has a groove for keeping the capillary 101 in place.
- the fixture 146 adjusts the path of the capillary 101 to be on the heat dissipation sheet 214.
- the groove may be provided in the second frame 204 at the position where the fixture 146 is arranged, or may be provided in both the second frame 204 at the position where the fixture 146 and the fixture 146 are arranged. ..
- the separator 208 (see FIG. 3) is a film or plate-shaped member, and has holes 300 formed in an number equal to or larger than the number of capillaries 101.
- the inner diameter of the hole 300 is slightly larger than the outer diameter of the capillary 101, for example, about ⁇ 1 mm.
- the separator 208 separates the capillaries 101 and prevents the capillaries 101 from being entangled and being densely packed into a bundle.
- the number of separators 208 may be increased or decreased according to the length of the capillary 101, and may be arranged at an appropriate position according to the length of the capillary 101.
- the capillary array unit 200 of this embodiment has a straightening vane 210 for adjusting the flow of air in the constant temperature bath 118 when installed in the constant temperature bath 118.
- the straightening vane 210 is a flat plate-shaped member. Since the straightening vane 210 does not limit the air flow path in the constant temperature bath 118 but adjusts the wind direction, it can be attached so as not to interfere with the attachment of the capillary array unit 200.
- FIG. 2B shows a state in which the second frame 204 is constrained by the holding portion 206. When the second frame 204 is held by the holding portion 206, the curved portion of the capillary 101 greatly bulges outward.
- the straightening vane 210 is provided on a part of the outer periphery of the capillary 101, and is connected to the capillary 101 regardless of whether the second frame 204 is held by the holding portion 206 or the second frame is connected to the liquid feeding mechanism 106. It is arranged at a position where it does not contact, that is, outside the range of motion of the capillary 101. Specifically, when the capillary array 102 is installed in the constant temperature bath 118, it is arranged below the straight line connecting the capillary head 110 and the detection unit 146, that is, below the moving straight line of the second frame. It is preferable to provide the capillary 101 at a position where the movement of the capillary 101 is small when the second frame 204 is moved.
- the straightening vane 210 Since the straightening vane 210 is arranged outside the range of motion of the capillary 101 when the second frame 204 is moved, it does not interfere with the attachment of the capillary array unit 200 and is easy to attach. As the straightening vane 210, a plate-shaped separator 146 that does not pass through the capillary 101 may be used. The wind direction control of the straightening vane 210 will be described in detail later.
- the first frame 202 has a finger hook 218, and the second frame 204 has a push-in portion 220 and a knob 222 (see FIG. 5).
- the pushing portion 220 is formed with a step so that the second frame 204 can be easily pushed in the direction of the arrow B with a finger.
- the mounting procedure of the capillary array unit 200 of the present invention will be described with reference to FIGS. 4, 5, 6A and 6B.
- the capillary array unit 200 is in a state where the second frame 204 of FIG. 2A is restrained by the holding portion 206 before being mounted on the constant temperature bath 118.
- the load header 112 is attached to the constant temperature bath 118.
- FIG. 4 shows a part of the lower side of the constant temperature bath 118 and the load header 112.
- the illustration of the capillary 101 attached to the load header 112 is omitted.
- the load header 112 is provided with a grip 400. The user grabs the grip 400 and inserts the load header 112 into the recess 402 of the constant temperature bath 118.
- grooves are provided on both side surfaces of the load header 112, and protrusions engaging with the grooves provided on both side surfaces of the load header 112 are provided on the inner surface of the recess 402 of the constant temperature bath 118. Is provided.
- the groove provided in the load header 202 and the protrusion provided on the inner surface of the recess 402 of the constant temperature bath 118 engage with each other, and the capillary array unit 200 is kept at a constant temperature. It is arranged in the tank 118 as shown in FIG.
- the capillary head 110 and the detection unit 146 are supported by the second frame 204, when the load header 112 is installed in the constant temperature bath 118, the capillary head 110 and the detection unit 146 hit the detection unit holder 500, which hinders the attachment. It can be prevented from becoming.
- the detection unit holder 500 fixes the detection unit 146 and positions the detection position 144 with respect to the irradiation detection unit 108. Further, since the capillary array unit 200 is arranged in the constant temperature bath 118 while the second frame 204 is held by the holding portion 206, it is possible to prevent the capillary head 110 and the block 128 from colliding with each other and hindering the attachment.
- the capillary array unit 200 in the constant temperature bath 118 without hindering the attachment of any of the electrophoresis apparatus 100.
- the capillary array unit 200 is installed in the constant temperature bath 118 simply by grasping the grip 400 and inserting the load header 112 into the recess 402 of the constant temperature bath 118.
- the capillary array unit 200 can be easily installed.
- the restraint by the holding portion 206 of the second frame 204 is released.
- the user puts his / her fingers on the finger rest 218 and the push-in portion 220, pushes the push-in portion 220 in the direction of the arrow B so as to sandwich the push-in portion 220 with each other's fingers, and releases the restraint of the second frame 204 by the holding portion 206.
- the second frame 204 is movable along the guide 212 (see FIG. 2A).
- the second frame 204 is slid in the direction of the arrow A with the knob 222 provided on the second frame 204.
- the capillary head 110 is inserted into the hole of the block 128 as shown in FIG. 6A.
- An enlarged view of the connection portion between the block 128 and the capillary head 110 is shown in FIG. 6B.
- the sealing surface 602 of the capillary head 110 is pressed against the block 128.
- the space between the capillary head 110 and the block 128 is sealed, and the capillary head 110 is fixed to the block 128.
- the capillary 101 and the liquid feeding mechanism 106 are connected by the above procedure.
- the user can attach the capillary head 110 to the block 128 without directly touching the capillary 101. Further, in this work, the capillary head 110 can be inserted into the hole of the block 128 simply by moving the second frame 204 along the guide 212, and the capillary head 110 can be easily attached to the block 128.
- the detection unit 146 is attached to the detection unit holder 500. As shown in FIG. 6A, when the capillary head 110 is connected to the block 128, the detection unit 146 is arranged in the detection unit holder 500. Therefore, the detection unit 146 can be attached to the detection unit holder 500 simply by closing the lid 502 of the detection unit holder 500, and the detection unit 146 can be easily attached.
- the procedure for removing the capillary array unit 200 is the reverse of the above-described mounting procedure.
- the lid 502 of the detection unit holder 500 is opened to release the fixing of the detection unit 146.
- the capillary head 110 is removed from the block 128. Loosen the set screw 600 to release the fixation of the capillary head 110 to the block 128.
- the capillary head 110 is removed from the hole of the block 128 by moving the second frame 204 along the guide 212 in the direction of the arrow B with the knob 222.
- the second frame 204 is further moved along the guide 212 in the direction of arrow B, then the finger rests 218 and the push-in portion 220 are each placed with fingers, and the push-in portion 220 is moved in the direction of arrow B so as to be sandwiched between the fingers.
- the capillary array unit 200 By pushing in, the second frame 204 is restrained by the holding portion 206. Finally, the load header 112 is removed from the constant temperature bath 118 with the grip 400. When removing the capillary array unit 200 as in the case of attachment, the capillary array unit 200 can be removed from the constant temperature bath 118 without disturbing the electrophoresis apparatus 100. As described above, the configuration of the capillary array unit 200 of the present invention makes it possible to easily attach / detach the capillary array unit 200.
- the second frame 204 of the capillary array unit 200 is in a state of protruding from the constant temperature bath 118.
- the constant temperature bath 118 is an air circulation type, and when controlling the temperature, it is necessary to keep it closed in order to shorten the temperature rising time and improve the temperature stability.
- FIG. 7A shows a state in which the capillary array unit 200 is mounted on the block 128 and the second frame 204 has passed through the constant temperature bath 118.
- the constant temperature bath 118 at the position where the second frame 204 passes is U-shaped.
- the capillaries 101 held by the second frame 204 and the second frame 204 are sandwiched between the lid provided in the constant temperature bath 118 (not shown) and the constant temperature bath 118. With this structure, the space of the constant temperature bath 118 is sealed.
- a heat radiating sheet 700 for sealing the constant temperature bath 118 and dissipating heat generated from the capillary 101 is provided on the contact surface between the constant temperature bath 118 and the capillary 101.
- the detection unit 146 slides by the second frame 204. If the detection unit 146 moves in contact with the heat radiating sheet 700, the heat radiating sheet 700 is rubbed, causing damage to the heat radiating sheet 700.
- the width of the second frame 204 in the vicinity of the detection unit 146 is wider than the width of the second frame 204 that passes through the constant temperature bath 118 when the capillary array unit 200 is mounted.
- the time when the detection unit 146 passes over the heat radiating sheet 700 is shown in FIG. 7B.
- Protrusions 701 (702) are provided on both side surfaces of the U-shaped portion of the constant temperature bath 118.
- FIG. 8A shows the air flow in the constant temperature bath 118 without the straightening vane 210
- FIG. 8B shows the air flow in the constant temperature bath 118 with the straightening vane 210.
- the capillary array unit 200 is arranged in the constant temperature bath 118, but FIGS. 8A and 8B show only the arrangement of the capillary 101 and the straightening vane 210.
- the constant temperature bath 118 is provided with a fan 800, and the fan 800 circulates the air in the constant temperature tank 118.
- the fan 800 has two functions, one is to agitate the air in the constant temperature bath to reduce the temperature variation, and the other is to dissipate Joule heat generated from the capillary 101 during electrophoresis.
- the air from the discharge port 802 of the fan 800 flows along the inner wall of the constant temperature bath 118 to form a swirling flow, as shown by the arrows. A part of the swirling flow is sucked into the suction port 804 of the fan 800.
- the capillary 101 passes through both the region where the air flow velocity of the constant temperature bath 118 is sufficient and the region where the flow velocity is low. Therefore, the amount of heat radiated from the capillary 101 differs depending on the location. As a result, the temperature of the capillary varies, which causes a decrease in analysis performance.
- FIG. 8B shows the flow of air in the constant temperature bath 118 when the rectifying plate 210 is provided on the capillary array unit.
- the capillary array unit 200 of this embodiment is provided with a straightening vane 210 according to the length of the capillary 101.
- the air emitted from the discharge port 802 of the fan 800 flows along the inner wall of the constant temperature bath 118 and hits the straightening vane 210.
- the wind direction is adjusted by the straightening vane 210 so that the wind hits the capillary 101, and it becomes possible to apply a sufficient wind to the entire capillary 101.
- the straightening vane 210 of this embodiment does not form a flow path along the capillary 101, but branches the flow of the wind so that the wind hits the capillary 101, and circulates the wind evenly in the constant temperature bath 118. Since the temperature of the entire surface of the constant temperature bath 118 is controlled by the Perche element, when forming a flow path through which the wind flows along the capillary 101, a region where the wind does not circulate is generated. Therefore, there is a possibility that a temperature difference will occur between the region where the wind circulates and the region where the wind does not circulate.
- the straightening vane 210 diverges the swirling flow of the air in the constant temperature bath 118 by the fan 800 to guide the air flow so that the capillary 101 is exposed to the wind. As a result, the swirling flow circulates throughout the constant temperature bath 118, so that the variation in the temperature distribution in the constant temperature tank 118 is reduced, and the analysis performance is improved. Further, by providing a gap without contacting the load header 112 and the straightening vane 210, it is possible to efficiently circulate the air throughout the constant temperature bath 118.
- two straightening vanes 210 are provided, but one or three or more may be provided.
- the straightening vane 210 outside the range of motion of the capillary 101, the flow of air in the constant temperature bath 118 is controlled and the mountability is improved.
- the position and number of the straightening vanes 210 may be changed according to the length of the capillary 101.
- the straightening vane 210 having the length of the capillary 101 can be attached and detached in accordance with the attachment and detachment of the capillary array unit 200.
- the straightening vane 210 may be directly mounted on the constant temperature bath 118 separately from the capillary array unit 200. Even when the straightening vane 210 is directly attached to the constant temperature bath 118, the capillary array unit 200 can be easily attached and detached by providing the straightening vane 210 outside the range of motion of the capillary 101.
- Electrophoretic apparatus 101: Capillary, 102: Capillary array, 104: Electrophoretic unit, 106: Liquid feeding mechanism, 108: Irradiation detection unit, 110: Capillary head, 112: Load header, 114: Cathode electrode, 116: Capillary cathode end, 118: Constant temperature bath, 120: Buffer solution, 122: Cathode side buffer container, 124: High voltage power supply, 126: Pump, 128: Block, 130: Polyphoresis, 132: Polyphoresis container, 134: Buffer solution 136 : Anode side buffer container, 138: Anode electrode, 140: Light source, 142: Detector, 144: Detection position, 146: Detection unit, 200: Capillary array unit, 202: First frame, 204: Second frame, 206: Holding part, 208: Separator, 210: Cathode, 212: Guide, 214 (215): Heat diss,
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Abstract
Description
図1は、電気泳動装置100の基本構成を示す概略図である。電気泳動装置100は、主にキャピラリ101を含むキャピラリアレイ102、電気泳動部104、キャピラリ101に泳動媒体である高粘性ポリマ溶液(以下、ポリマと称す)を送液する送液機構106、電気泳動によって分離された試料を光学的に検出する照射検出ユニット108を有する。
Claims (17)
- キャピラリと、
前記キャピラリを支持する支持体と、を有するキャピラリアレイユニットにおいて、
整流板を有し、
前記整流板は、平板状であることを特徴とするキャピラリアレイユニット。 - 請求項1のキャピラリアレイユニットにおいて、
前記整流板は前記キャピラリの外周の一部に設けられることを特徴とするキャピラリアレイユニット。 - 請求項1のキャピラリアレイユニットにおいて、
前記整流板は、前記キャピラリと接触しない位置に配置されることを特徴とするキャピラリアレイユニット。 - 請求項1のキャピラリアレイユニットにおいて、
前記支持体は、前記キャピラリを湾曲状に保持する第1支持部と、前記キャピラリを直線状に保持する第2支持部とを有し、
前記第1支持部は、前記第2支持部を所定の方向に移動させるためのガイドを有し、
前記整流板は、前記第2支持部が移動する直線上よりも下方に配置され、
前記第2支持部が移動する直線上に配置されないことを特徴とするキャピラリアレイユニット。 - 請求項4のキャピラリアレイユニットにおいて、
前記第1支持体は、前記第2支持部を着脱可能に保持する保持部を有することを特徴とするキャピラリアレイユニット。 - 請求項5のキャピラリアレイユニットにおいて、
前記整流板は、前記第1支持体が前記保持部に保持されているときに、前記整流板と、前記キャピラリは接触しないことを特徴とするキャピラリアレイユニット。 - 請求項4のキャピラリアレイユニットにおいて、
前記第1支持体は指かけを有し、
前記第2支持体は押圧部を有することを特徴とするキャピラリアレイユニット。 - 請求項4のキャピラリアレイユニットにおいて、
前記第2支持体はつまみを有することを特徴とするキャピラリアレイユニット。 - 請求項4のキャピラリアレイユニットにおいて、
前記キャピラリの一端は一部に穴または溝が設けられたキャピラリヘッドを有し、
前記第2支持体は前記穴または溝と係合する凸部を有することを特徴とするキャピラリアレイユニット。 - 請求項4のキャピラリアレイユニットにおいて、
前記第2支持体と前記キャピラリとの間は、放熱体を有することを特徴とするキャピラリアレイユニット。 - 請求項4のキャピラリアレイユニットにおいて、
前記キャピラリを前記第2支持体上に配置するための固定具を有し、
前記固定具または、前記第2支持体の前記固定具が配置される位置に、前記キャピラリを保持するための溝を有することを特徴とするキャピラリアレイユニット。 - 請求項1のキャピラリアレイユニットにおいて、
前記整流板と前記ロードヘッダの間は空隙を有することを特徴とするキャピラリアレイユニット。 - キャピラリと、前記キャピラリを支持する支持体とを有するキャピラリアレイユニットと、
前記キャピラリの一端が接続され、前記キャピラリに分離媒体を送液するための送液機構と、
前記キャピラリの一部に光を照射し、前記キャピラリ内の試料を測定する検出ユニットと、
前記キャピラリが内部に収容され、前記キャピラリの温調を行う恒温槽と、を有する電気泳動装置において、
前記恒温槽は、熱源と、前記恒温槽内に送風するファンとを有し、
前記恒温槽または前記キャピラリアレイユニットは、前記ファンにより送風された風の向きを調整するための整流板を有することを特徴とする電気泳動装置。 - 請求項13の電気泳動装置において、
前記整流板は、前記キャピラリの外周の一部に設けられることを特徴とする電気泳動装置。 - 請求項13の電気泳動装置において、
前記整流板は、前記ファンにより前記恒温槽内に生じた空気の旋回流を分岐することを特徴とする電気泳動装置。 - 請求項13の電気泳動装置において、
前記支持体は、前記キャピラリを湾曲状に保持する第1支持部と、前記キャピラリを直線状に保持する第2支持部とを有し、
前記恒温槽は、前記キャピラリが接触する位置の少なくとも一部に放熱シートを有し、
第2支持部の一部は、前記放熱シートを有した前記恒温槽の接触位置よりも幅が広いことを特徴とする電気泳動装置。 - 請求項13の電気泳動装置において、
前記送液機構の前記キャピラリの接続位置と、前記検出ユニットによる前記キャピラリの検出位置とは直線上に配置されており、
前記整流板は、前記直線の下方に配置されることを特徴とする電気泳動装置。
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