WO2021031750A1 - Sève de bouleau mélangée et son application dans une composition cosmétique de soin de la peau - Google Patents

Sève de bouleau mélangée et son application dans une composition cosmétique de soin de la peau Download PDF

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Publication number
WO2021031750A1
WO2021031750A1 PCT/CN2020/102231 CN2020102231W WO2021031750A1 WO 2021031750 A1 WO2021031750 A1 WO 2021031750A1 CN 2020102231 W CN2020102231 W CN 2020102231W WO 2021031750 A1 WO2021031750 A1 WO 2021031750A1
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Prior art keywords
birch sap
sap
mixed
northeast
birch
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PCT/CN2020/102231
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English (en)
Chinese (zh)
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王莎莎
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浙江养生堂天然药物研究所有限公司
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Publication of WO2021031750A1 publication Critical patent/WO2021031750A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/10—General cosmetic use
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures
    • A61K2800/592—Mixtures of compounds complementing their respective functions
    • A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18

Definitions

  • the invention relates to a mixed birch sap and its application in skin care cosmetic compositions.
  • Birch sap is the sap that flows from plants in the Betulaceae genus Betula. There are about 100 species of birch, mainly distributed in the northern temperate zone, with a few species distributed in the Arctic region. Birch sap is rich in polysaccharides, amino acids, vitamins, biotin, cytokinins, minerals, etc., which are needed by the human body. It has good moisturizing, whitening and anti-wrinkle effects to improve the skin, and promotes skin cell neutralization, moisturizing, whitening and anti-wrinkle Related protein expression, reduce transdermal water loss, brighten skin tone, improve skin elasticity, etc.
  • the present invention provides a mixed birch sap, which contains two or more birch sap from different origins.
  • Birch sap (also commonly referred to as white birch sap) is produced in Northeast China, North China, Northwest China, Southwest China, Japan, North Korea, Mongolia, Russia, Finland, Norway, Sweden, Estonia, Lithuania, Tru, Belarus, Ukraine, and the United States And Canada etc.
  • the origin is preferably Northeast China (hereinafter referred to as Northeast China), Finland, Belarus and Russia.
  • birch sap from northeast China is referred to as Northeast birch sap
  • birch sap from Finland is referred to as Finnish birch sap, and so on.
  • the mixed birch sap comprises northeastern birch sap and Finnish birch sap.
  • the mixed birch sap contains 20-80%, preferably 30-70%, more preferably 40-60% of Northeast birch sap and 80-20%, preferably 70-30%, More preferably 60-40% Finnish birch sap, based on the total weight of the mixed birch sap.
  • the mixed birch sap comprises Northeast birch sap and Camillian birch sap.
  • the mixed birch sap contains 20-80%, preferably 30-70%, more preferably 40-60% of Northeast birch sap and 80-20%, preferably 70-30%, More preferably 60-40% of administratian birch sap, based on the total weight of the mixed birch sap.
  • the mixed birch sap comprises Finnish birch sap and Camillian birch sap.
  • the mixed birch sap contains 20-80%, preferably 30-70%, more preferably 40-60% of Finnish birch sap and 80-20%, preferably 70-30%, More preferably 60-40% of administratian birch sap, based on the total weight of the mixed birch sap.
  • the mixed birch sap comprises northeastern birch sap and Russian birch sap.
  • the mixed birch sap contains 20-80%, preferably 30-70%, more preferably 40-60% of Northeast birch sap and 80-20%, preferably 70-30% , More preferably 60-40% Russian birch sap, based on the total weight of the mixed birch sap.
  • the present invention also relates to the use of the above-mentioned mixed birch sap in skin care cosmetic compositions.
  • the present invention relates to a skin care cosmetic composition, which comprises the mixed birch sap described in (A).
  • the content of the (A) mixed birch tree juice in the skin care cosmetic composition is about 18-98% by weight, preferably about 20-95% by weight, more preferably about 22-90% by weight, most preferably about 30-90 % By weight, based on the total weight of the skin care cosmetic composition.
  • the skin care cosmetic composition of the present invention does not contain any added water, but does not exclude moisture inherently contained in each component.
  • the skin care cosmetic composition of the present invention does not contain chelating agents such as EDTA salt, sodium polyphosphate, sodium metaphosphate, and gluconic acid.
  • the skin care cosmetic composition is a moisturizing cosmetic composition.
  • the moisturizing cosmetic composition significantly increases the moisture content of the subject's skin.
  • the skin care cosmetic composition is a whitening cosmetic composition.
  • the whitening cosmetic composition significantly improves the brightness of the subject's skin and reduces the content of melanin and red pigment in the skin.
  • the skin care cosmetic composition is an anti-wrinkle cosmetic composition.
  • the anti-wrinkle cosmetic composition due to the presence of the mixed birch sap, significantly reduces the number, area and depth of wrinkles in subjects.
  • the skin care cosmetic composition is an anti-inflammatory cosmetic composition.
  • the anti-inflammatory cosmetic composition significantly reduced the inflammation of the subject.
  • birch sap can be used in the form of a raw liquid or a concentrated liquid, for example, in a concentrated liquid of about 1.05-8 times, preferably about 1.1-4 times (referred to as about 1.05-8 times, preferably about 1.1- 4 times the concentrated birch sap) form to use.
  • the birch sap is commercially available.
  • the northeast birch sap can be purchased from Daxinganling Chaoyue Wild Berry Development Co., Ltd.
  • the Finnish birch sap can be purchased from MySeTo Oy Co., Ltd.
  • the Cambodian birch sap can be purchased from Jiulu Fruit and vegetable factories
  • Russian birch sap can be purchased from Healthystream Co., Ltd.
  • Concentrated birch sap is obtained by concentrating the above-mentioned commercial products. Concentration methods are known in the art, such as heating concentration, low-temperature vacuum concentration, membrane concentration and the like. In the present invention, the concentration is preferably carried out by a low-temperature freeze concentration or membrane concentration process. For example, the commercially available birch juice stock solution is input into a low-temperature drying equipment, the temperature is lowered to -40°C to -70°C, and the vacuum is applied to 0.1-30Pa. Concentrated in vacuum at low temperature to obtain concentrated birch sap with different concentration times.
  • the skin care cosmetic composition may optionally contain component (B) commonly used ingredients in skin care cosmetics, which include various commonly used ingredients known in the field of skin care cosmetics. Ingredients, examples of which include, but are not limited to, vehicles, active ingredients and excipients. Those skilled in the art can select the type and amount of component (B) according to needs. For example, the content of component (B) is usually about 2-82% by weight, based on the total weight of the cosmetic composition.
  • the vehicle is those known in the art, such as diluents, dispersants and carriers, and examples thereof include, but are not limited to, ethanol, dipropylene glycol, butylene glycol, and the like.
  • the content of the vehicle in the cosmetic composition is known in the art, for example, it usually accounts for 0.5-20% of the total weight of component (B).
  • the active ingredients are those known in the art, and examples thereof include, but are not limited to, for example, emollients, moisturizers, whitening active ingredients, anti-wrinkle active ingredients, anti-inflammatory active ingredients, and the like.
  • emollients include, but are not limited to, olive oil, macadamia oil, sweet almond oil, grape seed oil, avocado oil, corn oil, sesame oil, soybean oil, peanut oil, white flower seed oil, safflower seed oil, Dogtooth rose hip oil, argan tree kernel oil, jojoba seed oil, sunflower seed oil, palm tree fruit oil, squalane, ethylhexyl palmitate, isopropyl myristate, hydrogenated polyisobutylene, isotene Hexane, isododecane, diethylhexyl carbonate, dioctyl carbonate, isopropyl lauroyl sarcosine, isononyl isononanoate, hydrogenated polydecene, glycerol tri(ethylhexanoate) , Cetyl alcohol ethyl hexanoate, bis-diethoxy diethylene glycol cyclohexane 1,
  • solid emollients include, but are not limited to, cetyl alcohol, stearyl alcohol, cetearyl alcohol, behenyl alcohol, scylitol, lauric acid, myristic acid, palmitic acid, stearic acid, beeswax, candelilla Tree wax, carnauba wax, lanolin, ozokerite, jojoba seed wax, paraffin wax, microcrystalline wax, hydrogenated rice bran wax, hydrogenated coconut oil glycerides, glyceryl behenate/eicosanate, myristyl alcohol One or more of myristate, bis-diglyceride polyacyl adipate-2, shea butter, and muluxing palm seed butter.
  • the content of the emollient in the cosmetic composition is known in the art, for example, it usually accounts for about 1-50% of the total weight of component (B).
  • moisturizer examples include, but are not limited to, glycerin, diglycerin, butylene glycol, propylene glycol, 1,3-propanediol, dipropylene glycol, 1,2-pentanediol, polyethylene glycol-8, polyethylene glycol Alcohol-32, methylglucitol-10, methylglucitol-20, PEG/PPG-17/6 copolymer, glycerol-7, glycerol-26, glycerol glucoside, PPG-10 methyl glucose ether, PPG-20 methyl glucose ether, PEG/PPG/polybutylene glycol-8/5/3 glycerin, sucrose, trehalose, rhamnose, mannose, raffinose, Betaine, erythritol, xylitol, urea, glyceryl polyether-5 lactate, sodium hyaluronate, hydrolyzed sodium hyaluronate, acetyl
  • the whitening active ingredients include, but are not limited to, kojic acid, ascorbyl glucoside, arbutin, tranexamic acid, nicotinamide, plant sterols, plant sterols/behenyl alcohol/octyldecanol lauroyl glutamate, phenyl ethyl Resorcinol, turmeric root extract, birch bark extract, ceramide 2, ceramide 3, acetyl sphingosine, resveratrol, palm tree bark extract, coleus forskohlii root Extract, pepper seed extract, ubiquinone, cholesterol, cholesterol stearate, ascorbic acid, ascorbyl dipalmitate, tocopherol (vitamin E), tocopherol acetate, bisabolol, tetraisoascorbate Palmitate, pyridoxine dicaprylate, pyridoxine dipalmitate, retinyl palmitate, phytosterol/octyl
  • anti-wrinkle active ingredients include, but are not limited to, tocopherol (vitamin E), retinol, retinol palmitate, hydrolyzed collagen, hydrolyzed elastin, allantoin, yeast extract, oryzanol, tetrahydrocurcumin , Ellagic acid, ubiquinone, whey protein, acetyl hexapeptide-8, palmitoyl pentapeptide-4, salicyl sphingosine, birch sap concentrate, silymarin, silk sericin, sodium tocopherol phosphate , Ribonucleic acid (RNA), dipeptide diaminobutyrylbenzylamide diacetate, palmitoyl tripeptide-5, oligopeptide-1, hexapeptide-9, palmitoyl oligopeptide, palmitoyl tetrapeptide-7, Grape (VITIS VINIFERA) seed extract, PTEROCARPUS (MARSU),
  • anti-inflammatory active ingredients include, but are not limited to, dipotassium glycyrrhizinate, portulaca (PORTULACA OLERACEA) extract, oat (AVENA SATIVA) kernel extract (oatyl anthranilic acid), panthenol, allantoin , Biological sugar gum-1, ⁇ -glucan, fructan, SCUTELLARIA BAICALENSIS root extract, Aesculus HIPPOCASTANUM extract, bisabolol, 4-tert-butyl cyclohexanol, nerve Amide 3, hydrogenated lecithin, licorice root (GLYCYRRHIZA GLABRA) extract, hydrolyzed royal jelly protein, oryzanol, phytosphingosine, quercetin (quercetin), ginger root extract, rosemary leaf extract, white flower One or more of chamomile extract, calendula extract, centella asiatica extract, naringin, hesperidin, etc.
  • auxiliary materials include, but are not limited to, emulsifiers, thickeners, preservatives, perfumes and the like.
  • emulsifier examples include, but are not limited to, cetearyl oleate, sorbitan oleate, polysorbate-60, polysorbate-80, methylglucose sesquistearic acid Ester, PEG-20 methyl glucose sesquistearate, PEG-40 hydrogenated castor oil, PPG-26-butanol-26, PEG-4 polyglycerol-2 stearate, PEG-60 hydrogenated Castor oil, steareth-2, steareth-21, PPG-13-decyltetradeceth-24, cetearyl glucoside, PEG-100 stearate, glycerin Stearate, Glyceryl Stearate SE, Coco Glucoside, Ceteareth-25, PEG-40 Stearate, Polyglyceryl-3 Methyl Glucose Distearate, Glyceryl stearate citrate, polyglyceryl-10 stearate, polyglyceryl-10 myristate, polyglyce
  • the thickener examples include, but are not limited to, carbomers, acrylic acid (ester) and its derivatives, xanthan gum, gum arabic, polyethylene glycol-14M, polyethylene glycol-90M, succinyl poly One or more of high molecular polymers such as sugar, hydroxyethyl cellulose, hydroxypropyl cellulose, and hydroxypropyl methyl cellulose.
  • the content of the thickener in the cosmetic composition is known in the art, for example, it usually accounts for about 0.1-10% of the total weight of the component (B).
  • preservatives examples include, but are not limited to, methylparaben, propylparaben, phenoxyethanol, benzyl alcohol, phenethyl alcohol, bis(hydroxymethyl)imidazolidinylurea, potassium sorbate, sodium benzoate, chlorobenzene Glycerol, sodium dehydroacetate, caprylic hydroxamic acid, 1,2-hexanediol, 1,2-pentanediol, p-hydroxyacetophenone, caprylyl glycol, glyceryl caprylate, undecylenic acid
  • the content of the preservative in the cosmetic composition is known in the art, for example, it usually accounts for about 0.01-2% of the total weight of the component (B).
  • the skin care cosmetic composition of the present invention can be prepared by any suitable method known in the art.
  • it can be prepared by dissolving tanks, emulsifying pots, dispersers, transfer pumps and other equipment commonly used in the cosmetics field.
  • dissolving tanks When preparing, put the water-soluble substance into the water-phase dissolving kettle, and the oil-soluble substance into the oil-phase dissolving kettle, and heat the temperature of the two kettles to about 80°C.
  • a disperser Pre-dispersion. After the dissolution is completed, the oil phase and the water phase are transported to the emulsifying pot for homogenization and emulsification for about 5-15 minutes. After the emulsification is completed, the temperature of the material body is lowered to normal temperature, optional flavors, preservatives, etc. are added, and the pH of the product is adjusted as needed.
  • the products can be filled and shipped only after the relevant test indicators are qualified.
  • the above preparation methods can be deleted or adjusted according to dosage form requirements.
  • the cosmetic composition can be prepared into various dosage forms such as creams, creams, lotions, essences, etc. according to needs.
  • Example 1 The effect of birch sap mixture from different origins in moisturizing
  • This example aims to investigate the difference in moisturizing effects of birch sap from different origins. Among them, the effects of birch sap mixture on moisturizing-related genes, proteins and cell proliferation were investigated.
  • the inspection method is as follows:
  • Experimental reagents and consumables human primary keratinocytes, 6-well plates, keratinocyte culture medium, RNA extraction kit, reverse transcription kit, Trizol lysis solution, etc.
  • Inoculation Inoculate cells into a 6-well plate at a seeding density of 5E5/well, and incubate overnight in a 37°C and 5% CO 2 incubator;
  • RNA is extracted, reverse transcribed to cDNA, and then subjected to fluorescence quantitative PCR detection;
  • a Finnish birch sap or Northeast birch sap with a concentration of 50% refers to the use of pure water to dilute the original Finnish birch sap or Northeast birch sap to 50%.
  • b 2 times concentrated Finnish birch sap or 2 times concentrated Northeast birch sap refers to input Finnish birch sap or Northeast birch sap stock into low-temperature drying equipment, cool to -65°C, vacuum to 0.1Pa, and concentrate to 2 times .
  • Table 1 show that, compared with Finnish birch sap or Northeast birch sap alone, a mixture containing 30-70% Finnish birch sap and 70-30% Northeast birch sap can significantly increase keratinocytes
  • the expression level of moisturizing-related genes especially when the mixed birch sap contains 40-60% Finnish birch sap and 60-40% Northeast birch sap, cutin transglutaminase (TGM1) associated with the skin barrier ), epidermal tight junction proteins Z0-1 and CLDN, filaggrin (FLG) related to skin water retention capacity, and aquaporin (AQP3) related to skin water absorption capacity are all significantly higher than 100 % Northeast birch sap and 100% Finnish birch sap.
  • TGM1 cutin transglutaminase
  • FLG filaggrin
  • AQP3 aquaporin
  • Experimental reagents and consumables human primary keratinocytes, 12-well plates, keratinocyte culture medium, ELISA test kits with different indicators, etc.
  • test steps are as follows:
  • Inoculation Inoculate in a 12-well culture plate at an inoculation density of 2E5/well, cultivate in an incubator at 37°C and 5% CO2, and change the medium every two days;
  • T-Test method is used for statistical analysis.
  • a Finnish birch sap or elseian birch sap with a concentration of 50% refers to the use of pure water to dilute the original sap of Finnish birch sap or elsesian birch sap to 50%.
  • b 2 times concentrated Finnish birch sap or 2 times concentrated administratian birch sap refers to the input of Finnish birch sap or elsesian birch sap stock into low-temperature drying equipment, cooling to -65°C, vacuuming to 0.1Pa, and concentrating to 2 times .
  • Experimental reagents and consumables human primary keratinocytes, 96-well plate, keratinocyte culture medium, MTT kit.
  • the cells in logarithmic growth phase were routinely digested, seeded in a 96-well plate at a seeding density of 2E3/well, incubated in a 37°C, 5% CO2 incubator for 24 hours, added test samples, and set a negative control group (medium +cells), put in the incubator for 48 hours, suck out the culture solution, wash with PBS 3 times, suck dry PBS and add 100ul of 1mg/ml MTT solution to each well, continue to incubate for 4 hours in the 37°C, 5% CO2 incubator , Discard the supernatant, add 100ul DMSO to each well, measure the absorbance value (A) at 570nm with a microplate reader, and calculate the cell proliferation capacity according to the following formula:
  • Cell proliferation rate (%) (A administration group-A negative control group)/A negative control group*100%.
  • Example 2 The effect of birch sap mixture from different origins in whitening
  • This example aims to investigate the difference in whitening effects of birch sap from different origins. Among them, the effects of birch juice mixture on whitening-related genes, proteins, melanocytes, and 3D melanin skin models were investigated.
  • the inspection method is as follows:
  • Experimental reagents and consumables human primary melanocytes, 6-well plates, melanocyte culture medium, RNA extraction kit, reverse transcription kit, Trizol lysis solution, etc.
  • Inoculation Inoculate cells into a 6-well plate at a seeding density of 5E5/well, and incubate overnight in an incubator at 37°C and 5% CO2;
  • RNA is extracted, reverse transcribed to cDNA, and then subjected to fluorescence quantitative PCR detection;
  • the results in Table 4 show that, compared with individual Northeast birch sap or Finnish birch sap, a mixture containing 30-70% Northeast birch sap and 70-30% Finnish birch sap can significantly inhibit the synthesis and transport of melanin.
  • the expression of related genes especially when the mixed birch sap contains 40-60% Northeast birch sap and 60-40% Finnish birch sap, melanin synthesis related genes TYR, TYR1, TYR2, PMEL 17, MIFT M
  • the expression levels of MIFT B, melanin transport related genes Rab-27a and Myosin va are significantly lower than 100% Northeast birch sap and 100% Finnish birch sap.
  • Experimental reagents and consumables human primary melanocytes, 12-well plates, melanocyte culture medium, ELISA test kits with different indicators, etc.
  • test steps are as follows:
  • Inoculation Inoculate in a 12-well culture plate at an inoculation density of 2E5/well, cultivate in an incubator at 37°C and 5% CO2, and change the medium every two days;
  • T-Test method is used for statistical analysis.
  • Experimental reagents and consumables human primary melanocytes, 12-well plate, melanocyte culture medium, NaOH lysis solution.
  • Inoculation Inoculate in a 12-well culture plate at an inoculation density of 2E5/well, culture in a 37°C, 5% CO2 incubator, and change the medium every two days.
  • melanin inhibition rate (% ) (A negative control group-A administration group)/A negative control group*100%
  • Table 6 show that, compared with individual Northeast birch sap or Russian birch sap, a mixture containing 30-70% Northeast birch sap and 70-30% Russian birch sap can significantly inhibit melanocytes The production of melanin. Especially when the mixed birch sap contains 30-60% Northeast birch sap and 70-40% Russian birch sap, it can significantly inhibit the production of melanin in melanocytes.
  • Experimental reagents and consumables 3D melanin skin model (made in the laboratory), model culture medium, NaOH lysis solution.
  • 3D model construction use keratinocytes and melanocytes to construct a 3D skin model.
  • UVB irradiation treatment (UVB: 50mJ/cm2) is carried out every day, and then a certain amount of samples are respectively coated on the corresponding model surface.
  • the model control group only Cloth model culture medium, each group of 6 replicate holes, smear once a day, the total duration of action is 4 days.
  • a Finnish birch sap or elseian birch sap with a concentration of 50% refers to the use of pure water to dilute the original sap of Finnish birch sap or elsesian birch sap to 50%.
  • b 1.5 times concentrated Finnish birch sap or 1.5 times concentrated administratian birch sap refers to input Finnish birch sap or elsesian birch sap raw liquid into low-temperature drying equipment, cool to -65°C, vacuum to 0.1Pa, and concentrate to 1.5 times .
  • results in Table 7 also show that the Finnish birch sap and the Cambodiaian birch sap are concentrated 1.5 times and then mixed at a ratio of 1:1, which can significantly inhibit the production of melanin in melanocytes; but when the Finnish birch sap is mixed with The Ecuadorian birch sap is diluted with pure water and then mixed in proportion, and its melanin inhibition rate is significantly reduced.
  • Example 3 The effect of birch sap mixture from different origins in anti-wrinkle and anti-aging
  • This example aims to investigate the difference in anti-wrinkle and anti-aging effects of birch sap from different origins. Among them, the effects of birch sap mixture on anti-wrinkle-related genes, proteins, and 3D skin models were investigated.
  • the inspection method is as follows:
  • Experimental reagents and consumables human primary fibroblasts, 6-well plates, fibroblast culture medium, RNA extraction kit, reverse transcription kit, Trizol lysis solution, etc.
  • Inoculation Inoculate cells into a 6-well plate at a seeding density of 5E5/well, and incubate overnight in a 37°C and 5% CO 2 incubator;
  • RNA is extracted, reverse transcribed to cDNA, and then subjected to fluorescence quantitative PCR detection;
  • the 2- ⁇ CT method is used for calculation of the results, and the T-Test method is used for statistical analysis.
  • Table 8 show that a mixture containing 30-70% Northeast birch sap and 70-30% Finnish birch sap can significantly increase the basal fibroblast sap compared with individual Northeast birch sap or Finnish birch sap.
  • the gene expression levels of VII, elastin, and laminin were significantly higher than 100% Northeast birch sap and 100% Finnish birch sap.
  • results in Table 8 also show that the concentration of Northeast birch sap and Finnish birch sap at a ratio of 6:4 after being concentrated 1.2 times can significantly increase the expression of senescence-related genes in basal fibroblasts;
  • the birch sap and Finnish birch sap were diluted with pure water and then mixed in proportion, and the expression of senescence-related genes increased significantly.
  • Experimental reagents and consumables human primary fibroblasts, 12-well plates, fibroblast culture medium, ELISA test kits with different indicators, etc.
  • test steps are as follows:
  • Inoculation Inoculate in a 12-well culture plate at an inoculation density of 2E5/well, cultivate in an incubator at 37°C and 5% CO2, and change the medium every two days;
  • results in Table 9 also show that the concentration of Northeast birch sap and Camillian birch sap at a ratio of 6:4 after being concentrated 1.2 times can significantly increase the expression of senescence-related proteins in basal fibroblasts;
  • the birch sap and Cambodiaian birch sap were diluted with pure water twice and then mixed in proportion, and the expression of senescence-related proteins was significantly increased.
  • Experimental reagents and consumables 3D full-thickness skin model (made in the laboratory), ELISA test kits with different indicators, MTT kits, etc.
  • test steps are as follows:
  • Modeling and drug administration When the model leaves the factory, that is, day 0, SSUV irradiation treatment (UVA: 30J/cm2; UVB: 50mJ/cm2) is carried out every day, and then a certain amount of samples are respectively coated on the corresponding model surface ,
  • the model control group is only coated with the model culture medium, each group has 6 multiple holes, smeared once a day, the total duration of action is 4 days;
  • T-Test method is used for statistical analysis.
  • a Finnish birch sap or elseian birch sap with a concentration of 50% refers to the use of pure water to dilute the original sap of Finnish birch sap or elsesian birch sap to 50%.
  • b 1.2 times concentrated Finnish birch sap or 1.2 times concentrated administratian birch sap refers to input Finnish birch sap or elsesian birch sap stock into low-temperature drying equipment, cool to -65°C, vacuum to 0.1Pa, and concentrate to 1.2 times .
  • This example aims to investigate the anti-inflammatory effects of birch sap from different origins. Among them, the effects of birch sap mixture on inflammation-related animal models were investigated.
  • the inspection method is as follows:
  • Test animal zebrafish 5 days after fertilization (5dpf)
  • Sample group to be tested a mixture of different proportions of Northeast birch sap and Finnish birch sap + 15 ⁇ g/ml SP.
  • Model group 0% 100% Northeast Birch Sap 64.9% 70% Northeast birch sap + 30% Finnish birch sap 82.6%* 60% Northeast Birch Sap + 40% Finnish Birch Sap 91.2%** 50% Northeast Birch Sap+50% Finnish Birch Sap 100%** 40% Northeast Birch Sap + 60% Finnish Birch Sap 93.7%** 30% Northeast birch sap + 70% Finnish birch sap 86.2%* 100% Finnish birch sap 69.7%
  • the zebrafish mast cell allergy model contains 30-70% Northeastern birch sap and 70-30% Finnish birch sap compared with individual Northeast birch sap or Finnish birch sap.
  • the mixture can significantly inhibit the degranulation of zebrafish mast cells.
  • the mast cell degranulation inhibition rate is significantly better than 100% Northeast birch sap and 100%.
  • Test animal ICR mouse, male
  • Test reagents 10% sodium dodecyl sulfate solution (SDS), 1% hydrocortisone ointment, depilatory cream
  • mice were randomly grouped according to their body weight, each with 12 mice, divided into normal group, model group, positive group, and each sample group to be tested. All mice used depilatory cream to remove the skin and hair of about 2*2cm area on the mouse abdomen. No other treatments were done for the normal group.
  • the model group, the positive group, and each sample group to be tested were applied with 10% sodium lauryl sulfate solution for 5 consecutive days to complete the modeling. After modeling, the positive group was smeared with 1% hydrocortisone ointment 3 times a day for 2 consecutive days; the model group was not treated; each sample group to be tested was sprayed with the corresponding mixed birch sap, 3 times a day, 2 consecutive days day. On the 7th day of the experiment, the skin conditions of the experimental mice in each group were observed, and the serum of the mice was taken to determine the content of the inflammatory factor IL-1a.
  • a 50% concentration of Northeast birch sap or elseian birch sap refers to the use of pure water to dilute the Northeast birch sap or elseian birch sap to 50%.
  • b 4 times concentrated Northeast birch sap or 4 times concentrated effetian birch sap refers to the northeast birch sap or administratian birch sap raw liquid is fed into low-temperature drying equipment, cooled to -65°C, vacuumed to 0.1Pa, and concentrated to 4 times .
  • Test animal ICR mouse, male
  • Investigation indicators ear thickness index, ear weight, inflammation index interferon- ⁇ (IFN- ⁇ ), interleukin-4 (IL-4)
  • Test materials 1% 2,4-dinitrofluorobenzene (DNFB) acetone olive oil solution (4:1), 1% hydrocortisone ointment, hair removal cream
  • mice were randomly grouped according to their body weight, each with 12 mice, divided into normal group, model group, positive group, and each sample group to be tested.
  • Each group of experimental mice used depilatory cream to remove the skin and hair in the abdomen area of about 2*2cm.
  • the abdomen and ears of mice in the normal group were evenly coated with acetone olive oil solution for control.
  • Mice in the model group, positive group, and each sample group to be tested were smeared with 1% DNFB-containing acetone olive oil solution for sensitization and stimulation to establish a mouse model of allergic contact dermatitis.
  • model group After being challenged, the model group was sprayed with distilled water; the positive group was sprayed with 1% hydrocortisone ointment for 2 consecutive days, 3 times/day; each sample group to be tested was sprayed with the corresponding mixed birch sap, 3 times a day, continuous administration 2 days.
  • a Finnish birch sap or elseian birch sap with a concentration of 50% refers to the use of pure water to dilute the original sap of Finnish birch sap or elsesian birch sap to 50%.
  • b 4 times concentrated Finnish birch sap or 4 times concentrated administratian birch sap refers to input Finnish birch sap or elsesian birch sap stock into low-temperature drying equipment, cool to -65°C, vacuum to 0.1Pa, and concentrate to 4 times .
  • the above moisturizing cream composition is prepared as follows:
  • the raw material 7 is heated and dissolved with the raw material 10.
  • a Corneometer (Courage & Khazaka, Germany) was used to test the skin moisture content of 20 volunteers.
  • the test method is as follows:
  • composition sample Approximately 0.02 grams of each composition sample was dropped into the pre-marked square area on the volunteer's arm once a day in the morning and evening, and the sample was evenly applied to the area.
  • test results showed that compared with placebo, the average moisture content of the subjects' skin increased by 49%.
  • phase ingredient weight% A Mixed birch sap (50% Northeast birch sap +50% Russian birch sap) 68.17 To Sodium hyaluronate 0.03 To Xanthan gum 0.1 To Allantoin 0.1 To Methyl paraben 0.2 To PEG-11 methyl ether polydimethylsiloxane 0.1 To Panthenol 0.3 To Cetearyl olive oil and sorbitan olive oil 1 To glycerin 4 To Butanediol 5 To Acrylic (ester) type/vinyl isodecanoate cross-linked polymer 0.3 B Squalene 0.3 To Phytosterol/octyldodecanol lauroyl glutamate 0.3 To Tocopherol (Vitamin E) 0.2 To Triglyceride (ethylhexanoate) 3 C Polymethylsilsesquioxane 0.5 To Polydimethylsiloxane 1 D Tromethamine 0.4 E ascorbic acid 2 To Mixed
  • the above whitening essence composition is prepared as follows:
  • the phases A and B are heated to 80°C to dissolve evenly;
  • phase B 1. Keep at 80°C, slowly add phase B to phase A while stirring, after stirring for 10 minutes, homogenize at 10000rpm for 5 minutes;
  • VISIA-CR Using VISIA-CR to collect facial images of subjects under different light sources in different periods, use IPP software to analyze the skin glossiness, whiteness value L* and visible stain area of the same measurement area on the subject's face at different test time points.
  • the formula of the anti-wrinkle cream composition is shown in the following table:
  • the above-mentioned anti-wrinkle cream composition is prepared as follows:
  • Oil phase add 4, 6, 7, 8, 9, 11, 12, 13, 15, 21 raw materials into the oil phase pot, and heat to 80°C, dissolve and mix well;
  • Emulsification add the water phase and the oil phase to the emulsification tank, keep it at 80°C, homogenize emulsification at 3000rpm for 5 minutes, add No. 19 raw material after emulsification is complete;
  • step 6 Add the mixture of step 3 when stirring and cooling down to 40°C, stir evenly and then discharge to obtain the skin care cream composition.
  • anti-inflammatory spray composition is prepared as follows:
  • VISIA-CR Using VISIA-CR to collect the facial images of volunteers under the condition of using front and rear polarized light, and use IPP software to analyze the erythema area of the same measurement area at different test time points on the left and right cheeks of volunteers.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
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  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
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  • Cosmetics (AREA)

Abstract

L'invention concerne une sève de bouleau mélangée, comprenant au moins deux sèves de bouleau d'origines différentes. De plus, l'invention concerne également l'utilisation de la sève de bouleau mélangée dans une composition cosmétique de soin de la peau et une composition cosmétique de soin de la peau comprenant la sève de bouleau mélangée, notamment une composition cosmétique hydratante, une composition cosmétique de blanchiment, une composition cosmétique anti-rides et une composition cosmétique anti-inflammatoire.
PCT/CN2020/102231 2019-08-21 2020-07-16 Sève de bouleau mélangée et son application dans une composition cosmétique de soin de la peau WO2021031750A1 (fr)

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FR3129084A1 (fr) 2021-11-16 2023-05-19 L'Arboretum Composition cosmétique

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CN110731923B (zh) * 2019-07-30 2022-08-02 浙江养生堂天然药物研究所有限公司 增强的保湿化妆品组合物
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CN110876760B (zh) * 2019-11-28 2022-09-30 浙江养生堂天然药物研究所有限公司 具有促进伤口愈合和/或疤痕修复功效的皮肤外用组合物
CN112033944B (zh) * 2020-08-26 2023-03-28 南京新环检测科技有限公司 一种评价化妆品对皮肤刺激性的方法
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WO2022208005A1 (fr) * 2021-04-02 2022-10-06 Credo Composition cosmetique comprenant au moins un extrait de bouleau et au moins un extrait de bourgeon de hetre
FR3121359A1 (fr) * 2021-04-02 2022-10-07 Credo Composition cosmetique comprenant au moins un extrait de bouleau et au moins un extrait de bourgeon de hetre
FR3129084A1 (fr) 2021-11-16 2023-05-19 L'Arboretum Composition cosmétique
WO2023088980A1 (fr) 2021-11-16 2023-05-25 L'Arboretum Composition cosmetique comprenant de la sève de boulot, de la sève d'érable, de l'extrait de feuilles de saule et de l'extrait de feuilles de ginkgo biloba

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