WO2021017846A1 - Composition cosmétique anti-âge améliorée - Google Patents

Composition cosmétique anti-âge améliorée Download PDF

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Publication number
WO2021017846A1
WO2021017846A1 PCT/CN2020/102295 CN2020102295W WO2021017846A1 WO 2021017846 A1 WO2021017846 A1 WO 2021017846A1 CN 2020102295 W CN2020102295 W CN 2020102295W WO 2021017846 A1 WO2021017846 A1 WO 2021017846A1
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Prior art keywords
cosmetic composition
weight
test
aging cosmetic
wrinkle
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PCT/CN2020/102295
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English (en)
Chinese (zh)
Inventor
王莎莎
张正方
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浙江养生堂天然药物研究所有限公司
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Publication of WO2021017846A1 publication Critical patent/WO2021017846A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/35—Ketones, e.g. benzophenone
    • A61K8/355—Quinones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/60—Sugars; Derivatives thereof
    • A61K8/602—Glycosides, e.g. rutin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/67—Vitamins
    • A61K8/671—Vitamin A; Derivatives thereof, e.g. ester of vitamin A acid, ester of retinol, retinol, retinal
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/67—Vitamins
    • A61K8/678—Tocopherol, i.e. vitamin E
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73—Polysaccharides
    • A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures
    • A61K2800/592—Mixtures of compounds complementing their respective functions

Definitions

  • the present invention relates to an enhanced anti-aging cosmetic composition
  • an enhanced anti-aging cosmetic composition comprising (A) concentrated birch tree sap, the concentration of which is 1.05-8 times, preferably 1.1-4 times, more preferably 1.2-2 times, (B) From one or more of polypeptides, sodium hyaluronate, retinol, retinal, retinol palmitate, tocopherol, ubiquinone and adenosine.
  • Birch is a deciduous tree of the birch family. There are currently about 100 species in the world, mainly distributed in the northern temperate zone and the cold temperate zone. Among them, there are about 29 varieties in my country, mainly distributed in the northeast, northwest, north and southwest. Birch trees are mostly grown in remote mountainous areas with little human intervention and no industrial pollution. Birch sap (also called birch sap) is the fresh sap from the birch bark being cut or the trunk is drilled. It is colorless or light yellow, free of precipitation and impurities, and has a light birch fragrance. The birch sap contains a lot of sugars, amino acids, vitamins, biotin, cytokinins, trace mineral elements, aromatic oils, betulin, saponin and other compounds, which have good skin care and anti-aging effects.
  • Skin aging means that the skin's ability to protect and adjust to the body is reduced, and it cannot adapt to changes in internal and external environments, and changes in overall appearance such as color and shape. It is often manifested as reduced or disappeared skin elasticity, loose skin, increased wrinkles, and dry skin , Thinning, easy desquamation, itching, dull complexion, dull, and accompanied by stains and warts, affecting appearance and confidence. With the increasingly serious problem of population aging and the continuous improvement of living standards, consumers' anti-wrinkle awareness continues to increase, and more and more consumers are eager to use cosmetics to maintain their youthful appearance. According to statistics, anti-wrinkle products have become the category with the fastest growth rate and greatest potential in the Chinese cosmetics market.
  • the present invention relates to (A) concentrated birch sap and (B) selected from polypeptides, sodium hyaluronate, retinol, retinal, retinol palmitate, tocopherol, ubiquinone and adenosine
  • concentration factor of the concentrated birch sap is 1.05-8 times, preferably 1.1-4 times, more preferably 1.2-2 times.
  • the present invention provides an enhanced anti-aging cosmetic composition
  • an enhanced anti-aging cosmetic composition comprising (A) concentrated birch sap with a concentration ratio of 1.05-8 times, preferably 1.1-4 times, more preferably 1.2-2 times, And (B) one or more substances selected from polypeptide, sodium hyaluronate, retinol, retinal, retinol palmitate, tocopherol, ubiquinone and adenosine.
  • the anti-aging cosmetic composition of the present invention can promote the synthesis of collagen I, III, IV, VII, XVII, elastin, laminin, etc. in the extracellular matrix, reduce the expression of metal matrix protease MMP-1, and promote hyalurin Acid production strengthens the structural integrity and functionality of the epidermis, dermis, and dermal-epidermal junctions, improves skin elasticity, reduces wrinkles, and at the same time has the effects of improving the skin barrier, reducing transdermal water loss, anti-inflammatory and soothing, thereby providing significant anti-inflammatory effects. Ageing, especially anti-wrinkle formation effect.
  • the anti-aging cosmetic composition does not contain any added water, but does not exclude the moisture inherently contained in each component.
  • chelating agents such as EDTA salt, sodium polyphosphate, sodium metaphosphate, and gluconic acid are not added to the anti-aging cosmetic composition.
  • the birch sap involved in the present invention is obtained from the genus Betula, Betula alba, Betula pubescens, Betula pendula and Asian white birch (Betula platyphylla). Varieties.
  • the birch sap is a colorless, transparent, no-sediment-free, and no-drug sap that is artificially collected by drilling holes at the base of the trunk of the birch from thawing to early spring.
  • the birch sap is commercially available and used as it is, for example, it can be purchased from Daxinganling Chaoyue Wild Berry Development Co., Ltd.
  • the concentrated birch sap used in the present invention is obtained by concentrating the above-mentioned commercial products. Concentration methods are known in the art, such as heating concentration, low-temperature vacuum concentration, membrane concentration and the like. In the present invention, the concentration is preferably carried out by a low-temperature freeze concentration or membrane concentration process. For example, the commercially available birch juice stock solution is input into a low-temperature drying equipment, the temperature is lowered to -40°C to -70°C, and the vacuum is applied to 0.1-30Pa. Concentrate in vacuum at low temperature to obtain concentrated birch sap with a concentration ratio of 1.05-8 times.
  • the concentration ratio is 1.05-8 times, preferably 1.1-4 times, and more preferably 1.2-2 times concentrated birch sap and polypeptide.
  • the combination of sodium hyaluronate, retinol, retinal, retinol palmitate, tocopherol, ubiquinone, and/or adenosine can significantly provide the anti-aging effect of the cosmetic composition, especially anti-wrinkle formation.
  • the content of the (A) concentrated birch sap is about 18-98% by weight, preferably about 20-95% by weight, more preferably about 22-92% by weight, most preferably about 30-90% by weight, based on the anti-aging The total weight of the cosmetic composition.
  • the polypeptide in the component (B) is known in the art to promote the synthesis of matrix proteins, especially collagen, and may also increase the production of elastin, hyaluronic acid, glycosaminoglycans and fibronectin. Those that provide anti-wrinkle effects.
  • polypeptides examples include but are not limited to dipeptide-2, dipeptide-4, tripeptide-1, tetrapeptide-21, pentapeptide-3, hexapeptide-3, hexapeptide-8, Peptide-9, hexapeptide-10, palmitoyl dipeptide-5, palmitoyl dipeptide-7, palmitoyl tripeptide-1, palmitoyl tripeptide-5, palmitoyl tetrapeptide-3, palmitoyl tetrapeptide-7 , Palmitoyl pentapeptide-3, palmitoyl pentapeptide-4, palmitoyl hexapeptide-6, palmitoyl oligopeptide, acetyl tetrapeptide-3, acetyl tetrapeptide-5, acetyl tetrapeptide-9, acetyl Pentapeptide-3, acetylhexapeptide
  • the polypeptide is preferably selected from palmitoyl tripeptide-5, palmitoyl tetrapeptide-7, acetylhexapeptide-3, dipeptide diaminobutyrylbenzylamide diacetate, palmitoyl oligopeptide, hexapeptide- 9 and any combination thereof.
  • the above substances are all commercially available.
  • sodium hyaluronate in the component (B) examples include, but are not limited to, large molecular weight sodium hyaluronate (1800-2200KDa), medium molecular weight sodium hyaluronate (1000-1800KDa), small molecular weight sodium hyaluronate ( One or more of 10-1000KDa), hydrolyzed sodium hyaluronate (1-10KDa), acetylated sodium hyaluronate, etc.
  • Sodium hyaluronate can be prepared by methods known in the art, for example, firstly, sodium hyaluronate is prepared from a plant-derived medium (peptone, glucose, etc.) by fermentation. Sodium hyaluronate is also commercially available, for example, from Huaxi Freda Biomedical Co., Ltd.
  • retinol, retinal, retinol palmitate, tocopherol, ubiquinone, and adenosine in the component (B) are also ingredients known in the art, all of which are commercially available.
  • the content of the component (B) is about 0.005-10% by weight, preferably about 0.01-8% by weight, more preferably about 0.1-6% by weight, most preferably about 0.5-5% by weight, based on the anti-aging cosmetic composition
  • the total weight of the object is about 0.005-10% by weight, preferably about 0.01-8% by weight, more preferably about 0.1-6% by weight, most preferably about 0.5-5% by weight, based on the anti-aging cosmetic composition The total weight of the object.
  • the anti-aging cosmetic composition may optionally include components (C) commonly used in skin care cosmetics, examples of which include, but are not limited to, vehicles, active ingredients, and Accessories etc. These ingredients are known in the art, and those skilled in the art can select the type and amount of component (C) according to their needs.
  • the content of component (C) is usually about 0-70% by weight, based on the anti- The total weight of the aging cosmetic composition.
  • the vehicle is those known in the art, such as diluents, dispersants and carriers, and examples thereof include, but are not limited to, ethanol, dipropylene glycol, butylene glycol, and the like.
  • the content of the vehicle in the cosmetic composition is known in the art, for example, it usually accounts for 0.5-20% of the total weight of component (C).
  • the active ingredients are those known in the art, and examples thereof include, but are not limited to, for example, emollients, moisturizers, other anti-wrinkle active ingredients, and the like.
  • emollient examples include, but are not limited to, olive oil, macadamia nut oil, sweet almond oil, grape seed oil, avocado oil, corn oil, sesame oil, soybean oil, peanut oil, white flower seed oil, safflower seed oil, Dogtooth rose hip oil, argan tree kernel oil, jojoba seed oil, sunflower seed oil, palm tree fruit oil, squalane, ethylhexyl palmitate, isopropyl myristate, hydrogenated polyisobutylene, isotene Hexane, isododecane, diethylhexyl carbonate, dioctyl carbonate, isopropyl lauroyl sarcosine, isononyl isononanoate, hydrogenated polydecene, glycerol tri(ethylhexanoate) , Cetyl alcohol ethyl hexanoate, bis-diethoxy diethylene glycol cyclohexane
  • solid emollients include, but are not limited to, cetyl alcohol, stearyl alcohol, cetearyl alcohol, behenyl alcohol, scylitol, lauric acid, myristic acid, palmitic acid, stearic acid, beeswax, candelilla Tree wax, carnauba wax, lanolin, ozokerite, jojoba seed wax, paraffin wax, microcrystalline wax, hydrogenated rice bran wax, hydrogenated coconut oil glycerides, glyceryl behenate/eicosanate, myristyl alcohol One or more of myristate, bis-diglyceride polyacyl adipate-2, shea butter, and muluxing palm seed butter.
  • the content of the emollient in the cosmetic composition is known in the art, for example, it usually accounts for 1-50% of the total weight of component (C).
  • moisturizer examples include, but are not limited to, glycerin, diglycerin, butylene glycol, propylene glycol, 1,3-propanediol, dipropylene glycol, 1,2-pentanediol, polyethylene glycol-8, polyethylene glycol Alcohol-32, methylglucitol-10, methylglucitol-20, PEG/PPG-17/6 copolymer, glycerol-7, glycerol-26, glycerol glucoside, PPG-10 methyl glucose ether, PPG-20 methyl glucose ether, PEG/PPG/polybutylene glycol-8/5/3 glycerin, sucrose, trehalose, rhamnose, mannose, raffinose, Betaine, erythritol, xylitol, urea, glyceryl polyether-5 lactate, sodium hyaluronate, hydrolyzed sodium hyaluronate, acetyl
  • anti-wrinkle active ingredients include, but are not limited to, hydrolyzed collagen, hydrolyzed elastin, yeast extract, oryzanol, tetrahydrocurcumin, ellagic acid, whey protein, salicyl sphingosine, silymarin, silk gum Protein, tocopherol phosphate sodium, allantoin, ribonucleic acid (RNA), grape (VITIS VINIFERA) seed extract, palm tree (PTEROCARPUS) MARSUPIUM bark extract, tea (CAMELLIA SINENSIS) polyphenols, wine extract , Apple seed extract, FAGUS SYLVATICA bud extract, ADANSONIA DIGITATA extract, Artemia (ARTEMIA) extract, IRIS FLORENTINA root extract, Hesperidin , Ginsenosides, salvia miltiorrhiza (SALVIA MILTIORRHIZA) extract, nicotinamide, ursolic acid, lycopene,
  • auxiliary materials include, but are not limited to, emulsifiers, thickeners, preservatives, perfumes and the like.
  • emulsifier examples include, but are not limited to, cetearyl oleate, sorbitan oleate, polysorbate-60, polysorbate-80, methylglucose sesquistearic acid Ester, PEG-20 methyl glucose sesquistearate, PEG-40 hydrogenated castor oil, PPG-26-butanol-26, PEG-4 polyglycerol-2 stearate, PEG-60 hydrogenated Castor oil, steareth-2, steareth-21, PPG-13-decyltetradeceth-24, cetearyl glucoside, PEG-100 stearate, glycerin Stearate, Glyceryl Stearate SE, Coco Glucoside, Ceteareth-25, PEG-40 Stearate, Polyglyceryl-3 Methyl Glucose Distearate, Glyceryl stearate citrate, polyglyceryl-10 stearate, polyglyceryl-10 myristate, polyglyce
  • the thickener examples include, but are not limited to, carbomers, acrylic acid (ester) and its derivatives, xanthan gum, gum arabic, polyethylene glycol-14M, polyethylene glycol-90M, succinyl poly One or more of high molecular polymers such as sugar, hydroxyethyl cellulose, hydroxypropyl cellulose, and hydroxypropyl methyl cellulose.
  • the content of the thickener in the cosmetic composition is known in the art, for example, it usually accounts for 0.1-10% of the total weight of the component (C).
  • preservatives examples include, but are not limited to, methylparaben, propylparaben, phenoxyethanol, benzyl alcohol, phenethyl alcohol, bis(hydroxymethyl)imidazolidinylurea, potassium sorbate, sodium benzoate, chlorobenzene Glycerol, sodium dehydroacetate, caprylic hydroxamic acid, 1,2-hexanediol, 1,2-pentanediol, p-hydroxyacetophenone, caprylyl glycol, glyceryl caprylate, undecylenic acid
  • the content of the preservative in the cosmetic composition is known in the art, for example, it usually accounts for 0.01-2% of the total weight of component (C).
  • the anti-aging cosmetic composition of the present invention can be prepared by any suitable method known in the art.
  • it can be prepared by dissolving tanks, emulsifying pots, dispersers, and delivery pumps commonly used in the cosmetics field.
  • dissolving tanks, emulsifying pots, dispersers, and delivery pumps commonly used in the cosmetics field When preparing, put the water-soluble substance into the water-phase dissolving kettle, and the oil-soluble substance into the oil-phase dissolving kettle, and heat the temperature of the two kettles to about 80°C.
  • a disperser Pre-dispersion. After the dissolution is completed, the oil phase and the water phase are transported to the emulsifying pot for homogenization and emulsification for about 5-15 minutes. After the emulsification is completed, the temperature of the material body is reduced to normal temperature, optional flavors, preservatives, etc. are added, and the pH of the product is adjusted as necessary.
  • the products can be filled and shipped only after the relevant test indicators are qualified.
  • the anti-aging cosmetic composition can be made into various dosage forms such as creams, creams, lotions, and essences.
  • Example 1 Preparation of composition A, A1, B, B1, B2, B3, B4, C, C1, C2, D, D1, D2, E, E1, E2, F, F1, F2, J
  • compositions are as follows:
  • compositions are prepared as follows:
  • Example 2 Compositions A, A1, B, B1, B2, B3, B4, C, C1, C2, D, D1, D2, E, E1, E2, F, F1, F2, J are associated with aging Effects of gene expression
  • compositions A, A1, B, B1, B2, B3, B4, C, C1, C2, D, D1, D2, E, E1, E2, F, F1, F2 were tested and compared The effect of J1 on the expression of aging-related genes.
  • Experimental reagents and consumables human primary fibroblasts, 6-well plates, fibroblast culture medium, RNA extraction kit, reverse transcription kit, Trizol lysis solution, etc.
  • Inoculation Inoculate cells into a 6-well plate at a seeding density of 5E5/well, and incubate overnight in a 37°C and 5% CO 2 incubator;
  • RNA is extracted, reverse transcribed to cDNA, and then subjected to fluorescence quantitative PCR detection;
  • the 2- ⁇ CT method is used for calculation of the results, and the T-Test method is used for statistical analysis.
  • compositions can increase the expression levels of genes such as collagen I, III, IV, VII, elastin, and laminin, indicating that they contain birch sap, concentrated birch sap, palm
  • genes such as collagen I, III, IV, VII, elastin, and laminin
  • composition B2, C2, D2, E2, F2 and composition A1, B, C, D, E, and F respectively.
  • the combination of sodium hyaluronate, retinol, tocopherol, or ubiquinone significantly increased the gene expression level of each protein, which fully demonstrated the synergy between them; in particular, the comparison
  • the results of composition B2, B3, B4, C2, D2, E2, F2 and composition B1, C1, D1, E1, and F1 can clearly be seen that compared with the non-concentrated birch sap, the concentrated birch sap
  • the combination with polypeptide, sodium hyaluronate, retinol, tocopherol or ubiquinone significantly improves the anti-wrinkle effect of the cosmetic composition, and the concentration factor of the concentrated birch sap is about 1.05-8 times, preferably about 1.1-4 times .
  • Example 3 The influence of compositions G, G1, G2 on the expression of aging-related proteins
  • compositions G, G1, and G2 were further tested and compared.
  • Experimental reagents and consumables human primary fibroblasts, 12-well plates, fibroblast culture medium, ELISA test kits with different indicators, etc.
  • Inoculation Inoculate in a 12-well culture plate at an inoculation density of 2E5/well, culture at 37°C and 5% CO 2 in an incubator, and change the medium every two days;
  • T-Test method is used for statistical analysis.
  • compositions G1 and G2 containing birch sap or 1.2 times concentrated birch sap and adenosine significantly increased the protein expression levels of aging-related proteins in keratinocytes , Indicating that birch sap or concentrated birch sap has a synergistic effect with adenosine.
  • G1 and G2 it can be seen that the synergistic effect of concentrated birch sap on peptides is significantly higher than that of the original birch sap.
  • compositions are prepared as follows:
  • Oil phase add 6, 8, 9, 10, 11, 13, 14, 15, 16 raw materials into the oil phase pot, and heat each raw material to 80°C, dissolve and mix well;
  • Aqueous phase Heat the raw materials 1, 2, 3, 5, and 7 to 80°C, add the mixture of step 2, dissolve, and mix well;
  • Emulsification add the water phase and the oil phase to the emulsification tank, keep the temperature at 80°C, homogenize and emulsify at 3000rpm for 5 minutes, add No. 20 raw material after the emulsification is completed;
  • Example 5 Effect of composition H, H1, H2, J1 on photoaging 3D full-thickness skin model
  • Experimental reagents and consumables 3D full-thickness skin model (made in the laboratory), ELISA test kits with different indicators, MTT kits, etc.
  • Modeling and drug administration When the model leaves the factory, that is, day 0, SSUV irradiation treatment (UVA: 30J/cm2; UVB: 50mJ/cm2) is carried out every day, and then a certain amount of samples are respectively coated on the corresponding model surface , The model control is only coated with the model culture medium, each of the composition has 6 multiple holes, smeared once a day, and the total duration of action is 4 days;
  • compositions H1 and H2 can improve the tissue viability of the 3D full-thickness skin model, promote the expression of collagen IV at the junction of the true epidermis, and significantly reduce the expression of MMP-1 in the extracellular matrix.
  • H1 and H2 it can be seen that the synergistic effect of the concentrated birch sap on the polypeptide is significantly higher than that of the original birch sap.
  • Example 6 Effect of composition H and H2 on human skin morphology
  • subjects with wrinkle grades 1-5 were used as the research objects.
  • the test volunteers used H and H2 for 2 weeks and 4 weeks on the left and right side of the eye wrinkles, cheek moisture and TEWL changes, and evaluated the combination The effects of H and H2 on anti-wrinkle, increase skin moisture content and improve skin barrier function.
  • test samples After cleansing in the morning and evening, after using toner, take appropriate test samples and apply evenly on the left and right faces (including around the eyes). The original skin care habits remain unchanged throughout the test process, and only test samples are used to replace the previous creams used by the subjects. .
  • test equipment used is:
  • Subjects Subjects who meet the following conditions will be selected:
  • the test area has large areas of birthmarks, scratches, leukoplakia, pigmented moles, keloids and other skin signs that affect the test.
  • the test process is as follows:
  • test areas are the outer corners of the eyes, forehead and cheeks on both sides. Before the test, the subjects cleaned the face with facial cleanser, and wiped it with a dust-free absorbent paper towel after cleaning;
  • the number of wrinkles is used to characterize the number of wrinkles in the corners of the eyes. The higher the value, the more wrinkles, and vice versa.
  • the number of skin wrinkles gradually decreased after H2 was used, and the number of wrinkles decreased significantly at 4 weeks (p ⁇ 0.01). There was no significant difference in wrinkle area after H using for 4 weeks compared with before using (P>0.05). After 4 weeks of use, the difference between H2 and H is very significant (p ⁇ 0.01), indicating that H2 has the effect of reducing skin wrinkles.
  • Wrinkle depth is used to characterize the depth of wrinkles at the corners of the eyes. The higher the value, the deeper the wrinkles, and vice versa. The depth of skin wrinkles gradually decreased after H2 was used, and the wrinkle depth decreased significantly at 4 weeks (p ⁇ 0.01). There was no significant difference in wrinkle depth after H using for 4 weeks compared with before using (P>0.05). After 4 weeks of use, the difference between H2 and H was significant (p ⁇ 0.05), indicating that H2 has the effect of reducing skin wrinkles.
  • Wrinkle area is used to represent the total area of wrinkles in the corners of the eyes. The higher the value, the more wrinkles, and vice versa.
  • the skin wrinkle area gradually decreased after H2 use, and the wrinkle area decreased significantly at 4 weeks (p ⁇ 0.01). There was no significant difference in wrinkle area after H using for 4 weeks compared with before using (P>0.05). After 4 weeks of use, the difference between H2 and H is significant (p ⁇ 0.01), indicating that H2 has a significant effect of reducing skin wrinkles.
  • Moisture content is used to characterize the moisture content of the stratum corneum of the skin. The higher the value, the higher the skin moisture content, and vice versa, the lower the skin moisture content.
  • the skin moisture content gradually increased after H2 use, and the increase was very significant at 4 weeks (p ⁇ 0.01). H after 4 weeks of use, the skin moisture content was not significantly different from that before use (P>0.05). After 4 weeks of use, the difference between H2 and H is very significant (p ⁇ 0.01), indicating that H2 has the effect of increasing skin moisture content.
  • TEWL is used to characterize the skin barrier function. The higher the value, the worse the skin barrier function, and vice versa. The TEWL value gradually decreased after H2 use, and the decrease was significant at 4 weeks (p ⁇ 0.05). The TEWL value of H after 4 weeks of use was not significantly different from that before use (P>0.05). After 4 weeks of use, the difference between H2 and H was significant (p ⁇ 0.05), indicating that H2 has the effect of repairing the skin barrier function.
  • the above anti-wrinkle eye cream composition is prepared as follows:
  • Oil phase add raw materials 1, 3, 4, and 5 to the oil phase pot, and heat the above-mentioned raw materials to 80°C, dissolve, and mix well;
  • Emulsification add the water phase and the oil phase to the emulsification tank, keep the temperature at 80°C, homogenize and emulsify at 3000rpm for 5 minutes, add No. 12 raw material after the emulsification is completed;
  • step 5 Add No. 8 raw material, stir and add the mixture of step 2 when the temperature is lowered to 40°C, stir evenly and then discharge to obtain the anti-wrinkle eye cream composition.
  • Example 8 Preparation of anti-wrinkle emulsion composition
  • the formula of the anti-wrinkle emulsion composition is as follows:
  • the aforementioned anti-wrinkle emulsion composition is prepared as follows:
  • Oil phase Add raw materials No. 4, 5, 6, 7, 10, 12, 13, and 14 to the oil phase pot, and heat to 80°C, dissolve and mix well;
  • Emulsification add the water phase and the oil phase to the emulsification tank, keep the temperature at 80°C, homogenize emulsification at 3000rpm for 5 minutes, add No. 16 raw material after emulsification is completed;

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Abstract

La présente invention concerne une composition cosmétique anti-âge améliorée comprenant (A) une sève de bouleau concentrée, dont le facteur de concentration est d'environ 1,05 à 8, de préférence de 1,1 à 4 et de préférence encore de 1,2 à 2, et (B) une ou plusieurs substances choisies parmi un polypeptide, le hyaluronate de sodium, le rétinol, le rétinène, le palmitate de rétinol, le tocophérol, l'ubiquinone et l'adénosine.
PCT/CN2020/102295 2019-07-30 2020-07-16 Composition cosmétique anti-âge améliorée WO2021017846A1 (fr)

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