WO2021007853A1 - 一种全自动磁珠清洗装置 - Google Patents

一种全自动磁珠清洗装置 Download PDF

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Publication number
WO2021007853A1
WO2021007853A1 PCT/CN2019/096560 CN2019096560W WO2021007853A1 WO 2021007853 A1 WO2021007853 A1 WO 2021007853A1 CN 2019096560 W CN2019096560 W CN 2019096560W WO 2021007853 A1 WO2021007853 A1 WO 2021007853A1
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WO
WIPO (PCT)
Prior art keywords
mixing
liquid
plate
suction
cleaning
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PCT/CN2019/096560
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English (en)
French (fr)
Inventor
张立海
孙启鹏
王吉宁
王峰
苏桂香
董飒英
Original Assignee
烟台艾德康生物科技有限公司
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Application filed by 烟台艾德康生物科技有限公司 filed Critical 烟台艾德康生物科技有限公司
Priority to CN201980001160.9A priority Critical patent/CN112567250B/zh
Priority to PCT/CN2019/096560 priority patent/WO2021007853A1/zh
Publication of WO2021007853A1 publication Critical patent/WO2021007853A1/zh

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/50—Reuse, recycling or recovery technologies
    • Y02W30/62—Plastics recycling; Rubber recycling

Definitions

  • the invention relates to the technical field of magnetic bead cleaning in a fully automatic tubular chemiluminescence immunoassay instrument, and specifically relates to a fully automatic magnetic bead cleaning device.
  • Luminescence immunoassay is a new type of ultra-microanalysis technology established by combining luminescence analysis and immune response. This technology uses a chemical or bioluminescence system as an indicator system for the antigen-antibody reaction to quantitatively detect the antigen or antibody. This method has both the high sensitivity of luminescence analysis and the high specificity of antigen and antibody. Magnetic bead cleaning is one of the most important steps in luminescence immunoassay. The main purpose is to wash away the substances in the reaction solution that are not bound to the solid-phase antigen and antibody and the interfering substances that are non-specifically adsorbed on the solid-phase carrier during the reaction. The cleaning effect directly affects the accuracy of the test results.
  • the magnetic bead cleaning device in the prior art does not oscillate and mix after filling the cleaning liquid. Because the magnetic beads are adsorbed into a cluster, the cleaning liquid and the magnetic beads cannot be sufficiently mixed, resulting in poor cleaning effect of the magnetic beads and low cleaning efficiency
  • some magnetic bead cleaning devices disclose the mixing action, they generally use concentric rotation and mixing. The mixing operation takes a long time and requires special needles to add liquid. The equipment cost is high, the mixing effect is poor, and the cleaning efficiency is low;
  • the magnetic bead cleaning device in the prior art has a single function, and the magnetic bead cleaning process is complicated. It is necessary to implement magnetic attraction, liquid addition, liquid absorption, and pre-excitation liquid addition on different equipment. The cleaning efficiency is low and the equipment cost is high. .
  • the present invention provides a fully automatic magnetic bead cleaning device.
  • a fully automatic magnetic bead cleaning device including a bottom plate, a cleaning disk for placing a plurality of reaction cups, a rotating shaft module for driving the cleaning disk to rotate at a fixed time, and The magnetic bead adsorption module for adsorbing the magnetic beads in the cup, the liquid suction module for absorbing liquid in the reaction cup, the liquid addition module for adding liquid to the reaction cup, and the mixing for mixing the reaction cup Module
  • the rotating shaft module includes a hollow shaft fixedly installed on the bottom plate and a cleaning disk seat that is rotatably installed and concentrically installed on the hollow shaft, and the cleaning disk is fixedly installed on the cleaning disk seat concentrically;
  • the magnetic bead adsorption module includes a magnetic chuck with a plurality of magnets installed on the side, a plurality of grooves for avoiding position of the mixing head are arranged on the side of the magnetic chuck, and the bottom of the magnetic chuck is fixedly installed by a plurality of uprights On the bottom plate and directly below the cleaning disk, the bottom of the column is fixedly installed on the bottom plate through the column seat, and the center of the magnetic chuck is provided with a through hole for the cleaning disk seat to pass through;
  • the liquid suction module includes a fixed plate fixedly installed on the top of the hollow shaft, a liquid suction needle holder mounted up and down relative to the fixed plate, and a liquid suction unit installed on the liquid suction needle holder and passing through the fixed plate;
  • the liquid adding module includes a liquid adding unit installed on a fixed plate and a liquid adding mechanism fixed on a liquid suction needle holder;
  • the mixing module includes a mixing seat that is slidably mounted on a column, a mixing and oscillating motor, a mixing lifting motor, a number of mixing support units installed on the mixing seat, and a number of Mixing and pressing unit;
  • the mixing lifting motor is fixedly installed on the bottom plate, the mixing lifting motor rod of the mixing lifting motor is fixedly installed on the mixing base;
  • the mixing and oscillating motor is fixedly installed on the mixing base
  • a mixing main pulley is installed at the end of the motor rod of the mixing and oscillating motor, and the mixing main pulley is connected to a plurality of mixing slave pulleys through a mixing timing belt, and the mixing support
  • the unit and the mixing and pressing unit are coaxial in the vertical direction;
  • the mixing support unit includes a plurality of bearing seats fixedly installed on the mixing seat and a mixing shaft concentrically installed on the bearing seat through the bearing, and the mixing shaft and the mixing pulley are fixedly installed coaxially,
  • the mixing support head is eccentrically rotatably mounted on the upper part of the mixing shaft through a bearing, an anti-rotation plate is installed on the side of the mixing support head, an anti-rotation rod is installed vertically on the bearing seat, and the anti-rotation plate is provided for preventing A long hole penetrated by the rotating rod, a top groove is arranged on the top of the mixing support head, and the top groove is arranged as an arc surface;
  • the mixing and pressing unit includes a T-shaped and hollow mixing cover, a spring sleeved on the mixing cover, and a spring retainer ring for the shaft.
  • the hollow structure of the mixing cover can allow the dosing needle to pass through,
  • the spring is installed between the mixing cover and the fixing plate, and the elastic retaining ring for the shaft is installed after the mixing cover passes through the fixing plate.
  • the outer diameter of the elastic retaining ring for the shaft is larger than the installation of the mixing cover on the fixing plate Hole, the bottom of the mixing cover is provided with a bottom groove, and the bottom groove is set as a truncated cone with an isosceles trapezoid in cross section.
  • the beneficial effects of the present invention are: by setting the rotating shaft module to drive the cleaning disk to rotate regularly, as well as the magnetic bead adsorption module, the liquid absorption module and the liquid addition module, the magnetic absorption, liquid absorption and liquid addition to the reaction cup on the cleaning disk are realized.
  • the operation of mixing, mixing, and pre-exciting liquid is realized, and the above operations are realized at the same time for different holes of the cleaning plate, so that the entire cleaning process can be completed by one rotation of the cleaning plate, and the cleaning efficiency is improved; there are several installations on the side.
  • the magnetic chuck of the magnet can absorb the magnetic beads in the reaction cup to the side of the reaction cup, and then perform the liquid absorption operation, which can avoid the magnetic beads in the prior art from being attracted to the bottom surface of the reaction cup due to the misabsorption of magnetic beads during liquid absorption. Avoid affecting the accuracy of the analysis data; by setting the mixing module, the mixing support unit can lift the reaction cup in situ by using the mixing lifting motor, without taking out the reaction cup and adding liquid at the same time. The steps are compact, cleaning time is saved, and equipment costs are saved.
  • the topped reaction cup is compressed and limited by the mixing and pressing unit, so as to ensure the stability of the reaction cup during the mixing operation and prevent the reaction cup from splashing during mixing.
  • the present invention can also be improved as follows.
  • the rotating shaft module further includes a power rotating mechanism that includes a rotating shaft motor mounted on the bottom plate through a rotating shaft motor support, a rotating shaft main pulley mounted on the rotating shaft motor rod, and a rotating shaft main pulley passing through
  • the rotating shaft is connected with the synchronous belt from the pulley, and the rotating shaft is fixedly installed concentrically with the cleaning disc seat, and the rotating shaft motor is installed on the bottom plate through the rotating shaft motor support.
  • the beneficial effect of adopting the above-mentioned further solution is that by setting the rotating shaft motor, the rotating shaft main pulley and the rotating shaft slave pulley, the rotating shaft motor drives the cleaning disc seat to rotate synchronously, thereby realizing the rotating action of the cleaning disc, which is for adding liquid, magnetic attraction, and suction.
  • the realization of various operations such as liquid and mixing provides a good foundation.
  • thirty-six cup positions for placing reaction cups are provided on the cleaning tray, including eight pick-and-place cup positions arranged in a clockwise order in a circle, one first filling position, and the first group of four magnetic suction positions , The first magnetic suction level, the first dosing and mixing position, the second group of five magnetic suction positions, the second magnetic suction level, the second dosing mixing position, the third group of five One magnetic suction level, the third magnetic suction liquid level, the fourth liquid adding and mixing position, the fourth group of five magnetic suction positions, the fourth magnetic suction liquid level and a pre-excitation liquid mixing position.
  • the beneficial effect of adopting the above-mentioned further scheme is that by setting the above-mentioned cup position, the repeated operations of adding liquid, magnetic attraction, liquid absorption, liquid addition and mixing to the reaction cup can be realized in sequence, and the magnetic beads in the reaction cup can be washed multiple times. Operation to improve the cleaning effect and cleaning efficiency of magnetic beads.
  • the liquid suction module further includes a lifting motor installed on the fixed plate through a motor bracket, the lifting motor rod of the lifting motor is fixedly installed with the liquid suction needle holder, and the liquid suction needle holder and the fixed plate are also A plurality of guide rods and guide sleeves which are relatively lifted and slidably installed are provided.
  • the guide sleeves are fixedly installed on the liquid suction needle holder. One end of the guide rod is fixed on the fixed plate and the other end passes through the guide sleeve.
  • the beneficial effect of adopting the above-mentioned further solution is that the lifting motor is set to drive the liquid suction needle holder to move up and down, so as to realize the operation of lowering the liquid suction needle and inserting the liquid suction needle into the reaction cup for liquid suction during liquid suction, which is beneficial to the fully automated operation of magnetic bead cleaning , Improve cleaning efficiency.
  • the liquid suction unit includes a contour screw with a bottom thread installed on the suction needle holder, a spring sleeved on the contour screw, a buffer plate installed between the spring and the suction needle holder, and a penetrating and fixing
  • the beneficial effect of adopting the above-mentioned further solution is that by setting the equal height screw, spring and buffer plate, when the cleaning liquid in the reaction cup is aspirated, if the suction needle touches the bottom of the reaction cup, the spring is compressed and the buffer The plate rises, so as to prevent the excessive impact of the liquid suction on the bottom of the reaction cup from damaging the reaction cup, which is beneficial to protect the reaction cup and reduce the equipment damage rate.
  • the liquid addition unit includes a liquid addition needle holder installed on the fixing plate and a liquid addition needle passing through the liquid addition needle holder and fixed, the liquid addition needle passes through the mixing cover, and the liquid addition mechanism includes The first dosing needle and the pre-priming liquid dosing needle set on the pipette needle holder.
  • the beneficial effect of adopting the above-mentioned further solution is that the liquid addition unit is fixed on the fixed plate to add liquid to the reaction cup before the mixing operation, and the first liquid addition needle and the pre-excitation liquid are added through the first liquid addition needle and the pre-priming liquid provided on the pipette needle holder.
  • the liquid needle is used for the first liquid filling and pre-priming liquid filling operations to the reaction cup, and the liquid adding unit and the liquid adding mechanism are set according to actual needs to realize the full automation of the liquid adding process.
  • a sensor frame is installed on the bottom plate, a mixing photoelectric switch, a motor monitoring photoelectric switch, and a zero-position sensor photoelectric switch are installed on the sensor frame, and a mixing seat corresponding to the position of the mixing photoelectric switch is installed.
  • a uniform induction sheet, a code disc corresponding to the position of the motor monitoring photoelectric switch is installed on the cleaning disc seat, and a zero position induction sheet corresponding to the position of the zero induction photoelectric switch is also installed on the cleaning disc seat.
  • the beneficial effect of adopting the above-mentioned further scheme is that by setting the mixing sensor and the mixing photoelectric switch, it is used to monitor the operation of the mixing seat and prevent the jamming of the mixing seat from occurring; by setting the motor to monitor the photoelectric switch and code The disc is used to monitor the operation of the cleaning disc holder to prevent the phenomenon of jamming of the cleaning disc holder; by setting the zero position sensor photoelectric switch and the zero position sensor chip to detect the zero position of the system.
  • the bottom surface of the mixing seat is provided with a plurality of tension wheels with adjustable positions, and a mixing timing belt is installed on the tension wheels.
  • the beneficial effect of adopting the above-mentioned further scheme is that by setting the tension wheel, the tightness of the mixing timing belt can be adjusted, ensuring the proper tension of the mixing timing belt, extending the service life of the mixing timing belt and improving the mixing Operational efficiency.
  • a tensioning plate is also provided on the rotating shaft motor, both ends of the tensioning plate are fixedly installed on the rotating shaft motor support, the tensioning plate is installed with a jacking screw, and one end of the jacking screw is for jacking the rotating shaft The other end of the motor is screwed on the tension plate.
  • the beneficial effect of adopting the above-mentioned further solution is that by setting a tension plate, the position of the rotating shaft motor is fine-tuned, thereby adjusting the tension of the rotating shaft synchronous belt.
  • connectors are installed on the liquid adding needle, the first liquid adding needle, and the pre-priming liquid adding needle, and the motor bracket is equipped with a connector bracket capable of placing several connectors.
  • the beneficial effect of adopting the above-mentioned further solution is that, by providing a connector, it is convenient to maintain and replace the dosing needle, and the maintenance cost is reduced.
  • Figure 1 is a schematic diagram of the overall three-dimensional structure of the present invention.
  • Figure 2 is a schematic diagram of the overall three-dimensional structure of the present invention.
  • Figure 3 is a schematic diagram of the front view of the overall frame of the present invention.
  • FIG. 4 is a schematic diagram of the three-dimensional structure of the overall frame of the present invention.
  • Figure 5 is a schematic diagram of the three-dimensional structure of the shaft module
  • Figure 6 is a schematic diagram of the three-dimensional structure of the liquid suction module
  • Figure 7 is a schematic diagram of the front view of the liquid suction module
  • Figure 8 is a schematic diagram of the three-dimensional structure of the liquid adding and mixing module
  • Figure 9 is a schematic diagram of the three-dimensional structure of a liquid adding and mixing module
  • Figure 10 is a schematic diagram of the front structure of the liquid adding and mixing module
  • Figure 11 is a schematic diagram of the three-dimensional structure of the mixing module
  • Figure 12 is a schematic diagram of the three-dimensional structure of the mixing module
  • FIG. 13 is a schematic diagram of the three-dimensional structure of the mixing supporting unit and the mixing pressing unit;
  • Figure 14 is a schematic top view of the structure of the mixing supporting unit and the mixing and pressing unit;
  • Figure 15 is a schematic sectional view of the structure at A-A in Figure 14;
  • Fig. 16 is a schematic diagram of a three-dimensional enlarged structure of the mixing shaft
  • Figure 17 is a schematic diagram of a three-dimensional structure of a liquid suction unit
  • Figure 18 is a schematic top view of a liquid suction unit
  • Figure 19 is a schematic sectional view of the structure at B-B in Figure 18;
  • Figure 20 is a schematic diagram of the cup position distribution of the cleaning tray.
  • Base plate 4. Rotating shaft motor, 5. Rotating shaft motor support, 6. Tensioning plate, 7. Rotating shaft timing belt, 8. Rotating shaft main pulley, 9. Rotating shaft slave pulley, 10. Code plate, 11 .Magnetic suction cup, 12. Reaction cup, 13. Washing plate, 14. Fixed plate, 15. Adding needle holder, 16. Suction needle holder, 17. Guide rod, 18. Guide sleeve, 19. Motor bracket, 20. Lifting motor, 21. Connector, 22. Connector bracket, 23. Guide flange, 24. Tube, 25. Suction needle, 26. Add liquid needle, 27. Mixing cover, 28. Magnet, 29. Mixing seat , 30. Mixing timing belt, 31. Guide tube, 32. Column, 33. Column base, 34.
  • Eccentric mounting hole, 131. Take and place cup position, 132. Fill level for the first time, 133. The first group of four magnetic positions, 134. The first magnetic suction liquid Position, 135. The first mixing position, 136. The second group of five magnetic suction positions, 137. The second magnetic suction liquid level, 138. The second liquid mixing position, 139. The third Group of five magnetic positions, 1310. The third magnetic suction level, 1311. The fourth liquid addition and mixing position, 1312. The fourth group of five magnetic positions, 1313. The fourth magnetic suction level , 1314. Add pre-excitation solution and mix well.
  • a full-automatic magnetic bead cleaning device includes a bottom plate with feet 34 at the bottom, a cleaning disk 13 for placing a plurality of reaction cups 12, a rotating shaft module for driving the cleaning disk 13 to rotate regularly, and a reaction cup 12
  • the magnetic bead adsorption module for adsorbing the magnetic beads, the liquid suction module for absorbing liquid in the reaction cup 12, the liquid addition module for adding liquid to the reaction cup 12, and the mixing operation on the reaction cup 12 Mixing module;
  • Thirty-six cup positions for placing the reaction cup 12 are provided on the cleaning tray 13, including eight pick-and-place cup positions 131 arranged in a clockwise order in a circle, a first filling level 132, and a first group of four magnetic positions.
  • the taking-and-placement cup position 131 includes a cup-taking position and a cup-placement position.
  • the cup-taking position arranged in a clockwise direction is in a position in front of the cup-placement position.
  • the above cup position is set to realize the repeated operations of adding liquid, magnetic attraction, liquid absorption, liquid addition and mixing to the reaction cup 12 in sequence, realize the operation of washing the magnetic beads in the reaction cup 12 multiple times, and improve the cleaning effect of the magnetic beads And cleaning efficiency.
  • the rotating shaft module includes a hollow shaft 37 fixedly installed on the bottom plate 1 and a cleaning disk seat 38 rotatably installed and concentrically installed on the hollow shaft 37, and the cleaning disk 13 is fixedly installed on the cleaning disk seat 38 concentrically;
  • the rotating shaft module also includes a power rotating mechanism that includes a rotating shaft motor 4 installed on the base plate 1 through a rotating shaft motor support 5, a rotating shaft main pulley 8 installed on the shaft of the rotating shaft motor 4, and a rotating shaft main pulley 8 8
  • the rotating shaft slave pulley 9 connected by the rotating shaft synchronous belt 7, the rotating shaft slave pulley 9 and the cleaning disc seat 38 are fixedly installed concentrically, by setting the rotating shaft motor 4, the rotating shaft main pulley 8 and the rotating shaft slave pulley 9, by the rotating shaft
  • the motor 4 drives the cleaning disc seat 38 to rotate synchronously, thereby realizing the rotating action of the cleaning disc 13, providing a good foundation for the realization of various operations such as liquid addition, magnetic attraction, liquid absorption and mixing; the rotating shaft motor 4 is
  • the magnetic bead adsorption module includes a magnetic chuck 11 with a number of magnets 28 installed on the side. A number of grooves are provided on the side of the magnetic chuck 11 to avoid the position of the mixing head 50.
  • the bottom of the magnetic chuck 11 passes A number of uprights 32 are fixedly installed on the bottom plate 1 and located directly below the cleaning tray 13. The bottom of the uprights 32 is fixedly installed on the bottom plate 1 through the upright seat 33, and the magnetic chuck 11 is provided in the center for the cleaning disk seat 38 to penetrate Through hole
  • the liquid suction module includes a fixed plate 14 fixedly installed on the top of the hollow shaft 37, a liquid suction needle holder 16 mounted up and down relative to the fixed plate 14, and liquid suction which is installed on the liquid suction needle holder 16 and passes through the fixed plate 14.
  • the liquid suction module also includes a lifting motor 20 mounted on the fixed plate 14 through a motor bracket 19, the lifting motor rod 42 of the lifting motor 20 is fixedly installed with the liquid suction needle holder 16 through a guide flange 23, so A number of guide rods 17 and guide sleeves 18 that are mounted relative to each other up and down and slidingly mounted between the suction needle holder 16 and the fixed plate 14 are fixedly installed on the suction needle holder 16, and the guide rod 17 One end is fixed on the fixed plate 14 and the other end passes through the guide sleeve 18.
  • the lifting motor 20 drives the liquid suction needle holder 16 to move up and down, so that the liquid suction needle 25 is lowered and inserted into the cuvette 12 for liquid suction.
  • the liquid suction unit includes a contour screw 56 with a bottom thread mounted on the pipette holder 16, a spring 48 sleeved on the contour screw 56, and a mounting
  • the liquid dosing module includes a liquid dosing unit installed on the fixed plate 14 and a liquid dosing mechanism fixed on the pipette frame 16; the liquid dosing unit includes a liquid dosing needle frame 15 mounted on the fixed plate 14 and The dosing needle 26 passes through the dosing needle holder 15 and is fixed, and the dosing needle 26 passes through the mixing cover 27.
  • the dosing mechanism includes the first dosing needle 58 and the first dosing needle 58 and The pre-priming liquid adding needle 59 is used to add liquid to the reaction cup 12 before the mixing operation through the liquid adding unit fixed on the fixed plate 14.
  • the excitation liquid addition needle 59 performs the first liquid addition and pre-excitation liquid addition operations to the reaction cup 12, and the liquid addition unit and the liquid addition mechanism are set according to actual needs to realize the full automation of the liquid addition process.
  • the mixing module includes a mixing seat 29 slidably mounted on the column 32 through a guide cylinder 31, a mixing oscillating motor 39, a mixing lifting motor 40, and a number of mixing supporting units installed on the mixing seat 29 And several mixing and pressing units installed on the fixed plate 14;
  • the mixing lifting motor 40 is fixedly installed on the bottom plate 1, and the mixing lifting motor rod 41 of the mixing lifting motor 40 is fixedly installed on the mixing seat 29;
  • the mixing and oscillating motor 39 is fixedly installed on the bottom surface of the mixing seat 29, the end of the motor rod of the mixing and oscillating motor 39 is installed with a mixing main pulley 51, and the mixing main pulley 51 passes
  • the mixing timing belt 30 is connected with a plurality of mixing slave pulleys 46, the mixing supporting unit and the mixing pressing unit are coaxial in the vertical direction; the bottom surface of the mixing seat 29 can be installed in several positions The tensioning wheel 60 is adjusted.
  • the mixing timing belt 30 is installed on the tensioning wheel 60.
  • the tightness of the mixing timing belt 30 can be adjusted to ensure proper tension of the mixing timing belt 30. Tightness, extending the service life of the mixing timing belt 30 and improving the efficiency of the mixing operation;
  • the mixing support unit includes a number of bearing seats 53 fixedly mounted on the mixing seat 29 and concentrically mounted on the bearing through the bearing
  • the mixing shaft 55 on the seat 53 is fixed and installed coaxially with the mixing slave pulley 46, and the mixing support 50 is eccentrically mounted on the eccentric mounting hole 551 on the upper part of the mixing shaft 55 through a bearing.
  • an anti-rotation plate 54 is installed on the side of the mixing support head 50, an anti-rotation rod 52 is vertically installed on the bearing seat 53, and an elongated hole for the anti-rotation rod 52 is provided on the anti-rotation plate 54.
  • the top of the mixing support 50 is provided with a top groove 501, and the top groove 501 is set as an arc surface;
  • the mixing and pressing unit includes a T-shaped and hollow mixing cover 27, which is sleeved in the mixing
  • the spring 48 on the cover 27 and the shaft spring retainer 49, the hollow structure of the mixing cover 27 allows the dosing needle 26 to pass through, and the spring 48 is installed between the mixing cover 27 and the fixing plate 14.
  • an elastic retaining ring 49 for the shaft is installed.
  • the outer diameter of the elastic retaining ring 49 for the shaft is larger than the mounting hole of the mixing cover 27 on the fixing plate 14.
  • the bottom of the mixing cover 27 A bottom groove 271 is provided, and the bottom groove 271 is set as a truncated cone with an isosceles trapezoid in cross section.
  • a sensor frame 36 is installed on the bottom plate 1, a mixing photoelectric switch 35, a motor monitoring photoelectric switch 44, and a zero-position sensor photoelectric switch 45 are installed on the sensor frame 36, and the mixing seat 29 is installed with a mixing photoelectric switch.
  • the mixing sensor 47 corresponding to the position of the switch 35, the cleaning disk base 38 is installed with the code disk 10 corresponding to the position of the motor monitoring photoelectric switch 44, and the cleaning disk base 38 is also installed corresponding to the position of the zero sensor photoelectric switch 45
  • the zero position sensor sheet 43 is set with the mixing sensor 47 and the mixing photoelectric switch 35 to monitor the operation of the mixing seat 29 to prevent the jamming of the mixing seat 29 from occurring; monitor the photoelectric switch by setting the motor 44 and code plate 10 are used to monitor the operation of the cleaning plate holder 38 to prevent the phenomenon of the cleaning plate holder 38 from being stuck; by setting the zero position sensor photoelectric switch 45 and the zero position sensor sheet 43 to detect the zero position of the system .
  • the dosing needle 26, the first dosing needle 58, and the pre-priming liquid dosing needle 59 are all equipped with a connector 21, and the motor bracket 19 is equipped with a connector bracket 22 that can place several connectors 21.
  • the connector 21 By setting the connector 21, it is convenient Maintain and replace the dosing needle 26, reducing maintenance costs.
  • the reaction cup 12 on the cleaning disc 13 can be magnetically attracted, liquid absorbed, liquid added and mixed.
  • the magnetic chuck 11 can absorb the magnetic beads in the reaction cup 12 to the side of the reaction cup 12, and then perform the liquid absorption operation, which can prevent the magnetic beads from being adsorbed to the bottom surface of the reaction cup 12 in the prior art, which may cause magnetic absorption during liquid absorption.
  • the mixing support unit is used to lift the reaction cup 12 in situ by using the mixing lifting motor 40, without taking out the reaction cup 12 and simultaneously Dosing operation, the operation steps are compact, cleaning time is saved, and the equipment cost is saved.
  • the lifting reaction cup 12 is compressed and limited by the mixing and pressing unit, so as to ensure the stability of the reaction cup 12 during the mixing operation and prevent reaction Splashing occurs when the cup 12 is mixed; by setting the mixing support 50 in the mixing support unit to be eccentrically rotating and mounted on the upper part of the mixing shaft 55, the mixing follower wheel is driven by the mixing and oscillating motor 39 46 drives the mixing shaft 55 to rotate, thereby driving the mixing support 50 to eccentrically rotate along the mixing shaft 55, so as to realize the compression limit on the top of the cuvette 12 and the rotation at the bottom.
  • This eccentric shaking and mixing method The magnetic beads and the cleaning solution are thoroughly mixed, the mixing effect is good, the mixing time is short, and the mixing cost is low.
  • the mixing lifting motor 40 moves to raise the mixing seat 29, so that the mixing support 50 rises to hold up the reaction cup 12, and after the liquid injection needle 26 injects the cleaning solution into the reaction cup, the mixing and shaking motor 39 moves and passes
  • the mixing main pulley 51 and the mixing slave pulley 46 drive the mixing shaft 55 to rotate, thereby driving the mixing support 50 to perform an eccentric rotation movement, thereby completing the eccentric shaking and mixing action of the reaction cup 12, and the next step is to enter the second group
  • the five magnetic attraction positions 136 perform magnetic attraction operation, and then reach the second magnetic attraction liquid level 137 for magnetic attraction and liquid absorption at the same time, and then rotate through the second liquid adding and mixing position 138 and the third group of five magnetic Suction position 139, third magnetic suction level 1310, fourth liquid addition and mixing position 1311, fourth group of five magnetic suction positions 1312, fourth magnetic suction liquid level 1313, and pre-excitation liquid mixing Position 1314 performs corresponding operations, and finally the reaction cup 12 reaches the cup taking position, and the cleaning process is completed. Every

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Abstract

公开了一种全自动磁珠清洗装置，包括底板（1）、用以放置若干个反应杯（12）的清洗盘（13）、用以驱动清洗盘（13）定时旋转的转轴模组、用以对反应杯（12）内的磁珠进行吸附的磁珠吸附模组、对反应杯（12）进行吸液的吸液模组、对反应杯（12）进行加液的加液模组和用以对反应杯（12）进行混匀操作的混匀模组，混匀模组包括滑动安装在立柱（32）上的混匀座（29）、混匀震荡电机（39）、混匀升降电机（40）、若干个安装在混匀座（29）上的混匀承托单元和若干个安装在固定板（14）上的混匀压紧单元，实现清洗盘旋转一周即可完成整个清洗过程，提高了清洗效率，将磁珠与清洗液进行充分混匀，混匀效果好，混匀时间短，混匀成本低。

Description

一种全自动磁珠清洗装置 技术领域
本发明涉及全自动管式化学发光免疫分析仪中磁珠清洗技术领域，并具体涉及一种全自动磁珠清洗装置。
背景技术
发光免疫分析是将发光分析和免疫反应相结合而建立的一种新型超微量分析技术。该技术利用化学或生物发光系统作为抗原抗体反应的指示系统，借以定量检测抗原或抗体的方法，这种方法兼具有发光分析的高灵敏性和抗原抗体的高度特异性。磁珠清洗是发光免疫分析中最重要的环节之一，主要目的是洗去反应液中没有与固相抗原与抗体结合的物质以及在反应过程中非特异性吸附于固相载体的干扰物质，其清洗效果直接影响检测结果的准确性。现有技术中的磁珠清洗装置在加注清洗液后无震荡混匀动作，由于磁珠被吸附成团状，清洗液与磁珠无法充分混匀，造成磁珠清洗效果差，清洗效率低；有的磁珠清洗装置虽然公开了混匀动作，但是一般采用同心旋转混匀，混匀操作时间较长，且需要配合特制针头加液，设备成本高，混匀效果差，清洗效率低；另外，现有技术中的磁珠清洗装置功能单一，磁珠清洗流程工序复杂，需要在不同设备上实现磁吸、加液、吸液、加预激发液等动作，清洗效率低，设备成本高。
发明内容
本发明针对现有技术存在的不足，提供一种全自动磁珠清洗装置。
本发明解决上述技术问题的技术方案如下：一种全自动磁珠清洗装置，包括底板、用以放置若干个反应杯的清洗盘、用以驱动清洗盘定时旋转的转轴模组、用以对反应杯内的磁珠进行吸附的磁珠吸附模组、对反应杯进行吸液的吸液模组、对反应杯进行加液的加液模组和用以对反应杯进行混匀操作的混匀模组；
所述转轴模组包括固定安装在底板上的中空轴和转动安装且同心安装在中空轴上的清洗盘座，所述清洗盘同心固定安装在清洗盘座上；
所述磁珠吸附模组包括侧面安装有若干个磁铁的磁吸盘，所述磁吸盘侧面设置若干个用以为混匀托头避位的凹槽，所述磁吸盘的底部通过若干个立柱固定安装在底板上且位于清洗盘的正下方，所述立柱的底部通过立柱座固定安装在底板上，所述磁吸盘中心设置可供清洗盘座穿过的通孔；
所述吸液模组包括固定安装在中空轴顶部的固定板、相对于固定板升降安装的吸液针架和安装在吸液针架上且穿过固定板的吸液单元；
所述加液模组包括安装在固定板上的加液单元以及固定在吸液针架上的加液机构；
所述混匀模组包括滑动安装在立柱上的混匀座、混匀震荡电机、混匀升降电机、若干个安装在混匀座上的混匀承托单元和若干个安装在固定板上的混匀压紧单元；所述混匀升降电机固定安装在底板上，所述混匀升降电机的混匀升降电机杆固定安装在混匀座上；所述混匀震荡电机固定安装在混匀座的底面上，所述混匀震荡电机的电机杆端部安装混匀主带轮，所述混匀主带轮通过混匀同步带与若干个混匀从带轮连接，所述混匀承托单元与混匀压紧单元在竖直方向上同轴心；
所述混匀承托单元包括若干个固定安装在混匀座上的轴承座和通过轴承同心安装在轴承座上的混匀轴，所述混匀轴与混匀从带轮同轴固定安装，所述混匀托头通过轴承偏心旋转安装在混匀轴上部，所述混匀托头侧面安装防转板，所述轴承座上垂直安装防转杆，所述防转板上设置可供防转杆穿透的长条孔，所述混匀托头顶部设置顶部凹槽，所述顶部凹槽设置为圆弧面；
所述混匀压紧单元包括截面为T型且中空的混匀盖、套装在混匀盖上的弹簧和轴用弹簧挡圈，所述混匀盖的中空结构可供加液针穿过，所述弹簧安装在混匀盖与固定板之间，所述混匀盖穿过固定板后安装轴用弹性挡圈，所述轴用弹性挡圈的外径大于固定板上混匀盖的安装孔，所述混匀盖底部设置底部凹槽，所述底部凹槽设置为截面为等腰梯形的锥台。
本发明的有益效果是：通过设置转轴模组驱动清洗盘定时旋转以及磁珠吸附模组、吸液模组和加液模组，实现对清洗盘上的反应杯进行磁吸、吸液、加液、混匀、加预激发液操作，且实现对清洗盘的不同孔位同时进行上述操作，从而实现清洗盘旋转一周即可完成整个清洗过程，提高了清洗效率；通过设置侧面安装有若干个磁铁的磁吸盘，实现将反应杯内的磁珠吸附到反应杯的侧面，然后进行吸液操作，可以避免现有技术中将磁珠吸附到反应杯底面而造成吸液时误吸磁珠，避免影响分析数据的准确性；通过设置混匀模组，通过借助混匀升降电机实现混匀承托单元对反应杯进行原位顶起，无需将反应杯取出且同时可以进行加液操作，操作步骤紧凑，节省清洗时间，节约设备成本，通过混匀压紧单元对顶起的反应杯进行压紧限位，从而保证混匀操作时反应杯的稳定性，防止反应杯混匀时发生外溅现象；通过将混匀承托单元中的混匀托头设置为偏心旋转安装在混匀轴上部，在混匀震荡电机的作用下驱动混匀从动轮带动混匀轴旋转，从而驱动混匀托头沿混匀轴偏心旋转运动，从而实现反应杯顶部压紧限位，底部作旋转运动的动作，该种偏心震荡混匀的方式将磁珠与清洗液进行充分混匀，混匀效果好，混匀时间短， 混匀成本低。
在上述技术方案的基础上，本发明还可以做如下改进。
进一步，所述转轴模组还包括动力旋转机构，所述动力旋转机构包括通过转轴电机支座安装在底板上的转轴电机、安装在转轴电机杆上的转轴主带轮和与转轴主带轮通过转轴同步带连接的转轴从带轮，所述转轴从带轮与清洗盘座同心固定安装，所述转轴电机通过转轴电机支座安装在底板上。
采用上述进一步方案的有益效果是，通过设置转轴电机以及转轴主带轮和转轴从带轮，由转轴电机驱动清洗盘座同步旋转，进而实现清洗盘的旋转动作，为加液、磁吸、吸液和混匀等各个操作的实现提供良好的基础。
进一步，所述清洗盘上设置三十六个用于放置反应杯的杯位，其中包括顺时针依次圆周排列的八个取放杯位、一个首次加液位、第一组四个磁吸位、第一个磁吸吸液位、第一个加液混匀位、第二组五个磁吸位、第二个磁吸吸液位、第二个加液混匀位、第三组五个磁吸位、第三个磁吸吸液位、第四个加液混匀位、第四组五个磁吸位、第四个磁吸吸液位和一个加预激发液混匀位。
采用上述进一步方案的有益效果是，通过设置上述杯位，依次实现对反应杯加液、磁吸、吸液、加液混匀的重复操作，实现对反应杯内的磁珠进行多次冲洗的操作，提高磁珠的清洗效果和清洗效率。
进一步，所述吸液模组还包括通过马达支架安装在固定板上的升降马达，所述升降马达的升降马达杆与吸液针架固定安装，所述吸液针架与固定板之间还设置若干个相对升降滑动安装的导向杆和导向套，所述导向套固定安装在吸液针架上，所述导向杆的一端固定在固定板上，另一端穿过导向套。
采用上述进一步方案的有益效果是，通过设置升降马达驱动吸液针架做升降运动，从而实现吸液时使吸液针下降插入反应杯进行吸液的操作，有利于磁珠清洗的全自动化操作，提高清洗效率。
进一步，所述吸液单元包括底部螺纹安装在吸液针架上的等高螺钉、套装在等高螺钉上的弹簧、安装在弹簧和吸液针架之间的缓冲板及穿透且固定在缓冲板上的吸液针，所述吸液针穿透吸液针架，所述吸液针的顶端设置软管。
采用上述进一步方案的有益效果是，通过设置等高螺钉、弹簧和缓冲板，当对反应杯内的清洗液进行吸液操作时，如果吸液针触碰到反应杯底，弹簧被压缩，缓冲板上升，从而防止吸液针对反应杯底的冲击力过大而损坏反应杯，有利于保护反应杯，降低设备 损坏率。
进一步，所述加液单元包括安装在固定板上的加液针架和穿过加液针架并进行固定的加液针，所述加液针穿过混匀盖，所述加液机构包括设置在吸液针架上的首次加液针和预激发液加液针。
采用上述进一步方案的有益效果是，通过设置固定在固定板上的加液单元对混匀操作前的反应杯进行加液，通过设置在吸液针架上的首次加液针和预激发液加液针，对反应杯进行首次加液和预激发液加液操作，根据实际需要设置加液单元和加液机构，实现加液过程的全自动化。
进一步，所述底板上安装感应器架，所述感应器架上安装混匀光电开关、电机监测光电开关和零位感应光电开关，所述混匀座上安装与混匀光电开关位置对应的混匀感应片，所述清洗盘座上安装与电机监测光电开关位置对应的码盘，所述清洗盘座上还安装与零位感应光电开关位置对应的零位感应片。
采用上述进一步方案的有益效果是，通过设置混匀感应片和混匀光电开关，用以监测混匀座的运行情况，防止发生混匀座卡死的现象发生；通过设置电机监测光电开关和码盘，用以监测清洗盘座的运行情况，防止发生清洗盘座卡死的现象发生；通过设置零位感应光电开关和零位感应片，用以检测系统零位。
进一步，所述混匀座底面安装若干个位置可调的张紧轮，所述张紧轮上安装混匀同步带。
采用上述进一步方案的有益效果是，通过设置张紧轮，实现对混匀同步带的松紧度进行调节，保证混匀同步带适宜的张紧度，延长混匀同步带的使用寿命和提高混匀操作的高效性。
进一步，所述转轴电机上还设置张紧板，所述张紧板两端固定安装在转轴电机支座上，所述张紧板上安装顶紧螺钉，所述顶紧螺钉的一端顶紧转轴电机，另一端螺纹安装在张紧板上。
采用上述进一步方案的有益效果是，通过设置张紧板，对转轴电机的位置进行微调，从而调节转轴同步带的张紧度。
进一步，所述加液针、首次加液针和预激发液加液针上均安装接头，所述马达支架上安装可放置若干个接头的接头支架。
采用上述进一步方案的有益效果是，通过设置接头，便于维护和更换加液针，降低维护成本。
附图说明
图1为本发明的整体立体结构示意图；
图2为本发明的整体立体结构示意图；
图3为本发明整体框架主视结构示意图；
图4为本发明整体框架立体结构示意图；
图5为转轴模组立体结构示意图；
图6为吸液模组立体结构示意图；
图7为吸液模组主视结构示意图；
图8为加液混匀模组立体结构示意图；
图9为加液混匀模组立体结构示意图；
图10为加液混匀模组主视结构示意图；
图11为混匀模组立体结构示意图；
图12为混匀模组立体结构示意图；
图13为混匀承托单元和混匀压紧单元的立体结构示意图；
图14为混匀承托单元和混匀压紧单元的俯视结构示意图；
图15为图14中A-A处的剖视结构示意图；
图16为混匀轴的立体放大结构示意图；
图17为吸液单元的立体结构示意图；
图18为吸液单元的俯视结构示意图；
图19为图18中B-B处的剖视结构示意图；
图20为清洗盘的杯位分布示意图。
图中1.底板，4.转轴电机，5.转轴电机支座，6.张紧板，7.转轴同步带，8.转轴主带轮，9.转轴从带轮，10.码盘，11.磁吸盘，12.反应杯，13.清洗盘，14.固定板，15.加液针架，16.吸液针架，17.导向杆，18.导向套，19.马达支架，20.升降马达，21.接头，22.接头支架，23.导向法兰，24.软管，25.吸液针，26.加液针，27.混匀盖，28.磁铁，29.混匀座，30.混匀同步带，31.导向筒，32.立柱，33.立柱座，34.支脚，35.混匀光电开关，36.感应器架，37.中空轴，38.清洗盘座，39.混匀震荡电机，40.混匀升降电机，41.混匀升降电机杆，42.升降马达杆，43.零位感应片，44.电机监测光电开关，45.零位感应光电开关，46.混匀从带轮，47.混匀感应片，48.弹簧，49.轴用弹簧挡圈，50.混匀托头，51.混匀主带轮，52.防转杆，53.轴承座，54.防转板，55.混匀轴，56.等高螺钉，57.缓冲板，58.首 次加液针，59.预激发液加液针，60.张紧轮，271.底部凹槽，501.顶部凹槽，551.偏心安装孔，131.取放杯位，132.首次加液位，133.第一组四个磁吸位，134.第一个磁吸吸液位，135.第一个加液混匀位，136.第二组五个磁吸位，137.第二个磁吸吸液位，138.第二个加液混匀位，139.第三组五个磁吸位，1310.第三个磁吸吸液位，1311.第四个加液混匀位，1312.第四组五个磁吸位，1313.第四个磁吸吸液位，1314.加预激发液混匀位。
具体实施方式
以下结合附图对本发明的原理和特征进行描述，所举实例只用于解释本发明，并非用于限定本发明的范围。
一种全自动磁珠清洗装置，包括底部设置支脚34的底板1、用以放置若干个反应杯12的清洗盘13、用以驱动清洗盘13定时旋转的转轴模组、用以对反应杯12内的磁珠进行吸附的磁珠吸附模组、对反应杯12进行吸液的吸液模组、对反应杯12进行加液的加液模组和用以对反应杯12进行混匀操作的混匀模组；
所述清洗盘13上设置三十六个用于放置反应杯12的杯位，其中包括顺时针依次圆周排列的八个取放杯位131、一个首次加液位132、第一组四个磁吸位133、第一个磁吸吸液位134、第一个加液混匀位135、第二组五个磁吸位136、第二个磁吸吸液位137、第二个加液混匀位138、第三组五个磁吸位139、第三个磁吸吸液位1310、第四个加液混匀位1311、第四组五个磁吸位1312、第四个磁吸吸液位1313和一个加预激发液混匀位1314，所述取放杯位131包括取杯位和放杯位，按照顺时针排列的取杯位处于放杯位的前面杯位的位置，通过设置上述杯位，依次实现对反应杯12加液、磁吸、吸液、加液混匀的重复操作，实现对反应杯12内的磁珠进行多次冲洗的操作，提高磁珠的清洗效果和清洗效率。
所述转轴模组包括固定安装在底板1上的中空轴37和转动安装且同心安装在中空轴37上的清洗盘座38，所述清洗盘13同心固定安装在清洗盘座38上；所述转轴模组还包括动力旋转机构，所述动力旋转机构包括通过转轴电机支座5安装在底板1上的转轴电机4、安装在转轴电机4杆上的转轴主带轮8和与转轴主带轮8通过转轴同步带7连接的转轴从带轮9，所述转轴从带轮9与清洗盘座38同心固定安装，通过设置转轴电机4以及转轴主带轮8和转轴从带轮9，由转轴电机4驱动清洗盘座38同步旋转，进而实现清洗盘13的旋转动作，为加液、磁吸、吸液和混匀等各个操作的实现提供良好的基础；所述转轴电机4上还设置张紧板6，所述张紧板6两端固定安装在转轴电机支座 5上，所述张紧板6上安装顶紧螺钉，所述顶紧螺钉的一端顶紧转轴电机4，另一端螺纹安装在张紧板6上，通过设置张紧板6，对转轴电机4的位置进行微调，从而调节转轴同步带7的张紧度。
所述磁珠吸附模组包括侧面安装有若干个磁铁28的磁吸盘11，所述磁吸盘11侧面设置若干个用以为混匀托头50避位的凹槽，所述磁吸盘11的底部通过若干个立柱32固定安装在底板1上且位于清洗盘13的正下方，所述立柱32的底部通过立柱座33固定安装在底板1上，所述磁吸盘11中心设置可供清洗盘座38穿过的通孔；
所述吸液模组包括固定安装在中空轴37顶部的固定板14、相对于固定板14升降安装的吸液针架16和安装在吸液针架16上且穿过固定板14的吸液单元；所述吸液模组还包括通过马达支架19安装在固定板14上的升降马达20，所述升降马达20的升降马达杆42通过导向法兰23与吸液针架16固定安装，所述吸液针架16与固定板14之间还设置若干个相对升降滑动安装的导向杆17和导向套18，所述导向套18固定安装在吸液针架16上，所述导向杆17的一端固定在固定板14上，另一端穿过导向套18，通过设置升降马达20驱动吸液针架16做升降运动，从而实现吸液时使吸液针25下降插入反应杯12进行吸液的操作，有利于磁珠清洗的全自动化操作，提高清洗效率；所述吸液单元包括底部螺纹安装在吸液针架16上的等高螺钉56、套装在等高螺钉56上的弹簧48、安装在弹簧48和吸液针架16之间的缓冲板57及穿透且固定在缓冲板57上的吸液针25，所述吸液针25穿透吸液针架16，所述吸液针25的顶端设置软管24，通过设置等高螺钉56、弹簧48和缓冲板57，当对反应杯12内的清洗液进行吸液操作时，如果吸液针25触碰到反应杯12底，弹簧48被压缩，缓冲板57上升，从而防止吸液针25对反应杯12底的冲击力过大而损坏反应杯12，有利于保护反应杯12，降低设备损坏率。
所述加液模组包括安装在固定板14上的加液单元以及固定在吸液针架16上的加液机构；所述加液单元包括安装在固定板14上的加液针架15和穿过加液针架15并进行固定的加液针26，所述加液针26穿过混匀盖27，所述加液机构包括设置在吸液针架16上的首次加液针58和预激发液加液针59，通过设置固定在固定板14上的加液单元对混匀操作前的反应杯12进行加液，通过设置在吸液针架16上的首次加液针58和预激发液加液针59，对反应杯12进行首次加液和预激发液加液操作，根据实际需要设置加液单元和加液机构，实现加液过程的全自动化。
所述混匀模组包括通过导向筒31滑动安装在立柱32上的混匀座29、混匀震荡电机39、混匀升降电机40、若干个安装在混匀座29上的混匀承托单元和若干个安装在固定 板14上的混匀压紧单元；所述混匀升降电机40固定安装在底板1上，所述混匀升降电机40的混匀升降电机杆41固定安装在混匀座29上；所述混匀震荡电机39固定安装在混匀座29的底面上，所述混匀震荡电机39的电机杆端部安装混匀主带轮51，所述混匀主带轮51通过混匀同步带30与若干个混匀从带轮46连接，所述混匀承托单元与混匀压紧单元在竖直方向上同轴心；所述混匀座29底面安装若干个位置可调的张紧轮60，所述张紧轮60上安装混匀同步带30，通过设置张紧轮60，实现对混匀同步带30的松紧度进行调节，保证混匀同步带30适宜的张紧度，延长混匀同步带30的使用寿命和提高混匀操作的高效性；所述混匀承托单元包括若干个固定安装在混匀座29上的轴承座53和通过轴承同心安装在轴承座53上的混匀轴55，所述混匀轴55与混匀从带轮46同轴固定安装，所述混匀托头50通过轴承偏心旋转安装在混匀轴55上部的偏心安装孔551上，所述混匀托头50侧面安装防转板54，所述轴承座53上垂直安装防转杆52，所述防转板54上设置可供防转杆52穿透的长条孔，所述混匀托头50顶部设置顶部凹槽501，所述顶部凹槽501设置为圆弧面；所述混匀压紧单元包括截面为T型且中空的混匀盖27、套装在混匀盖27上的弹簧48和轴用弹簧挡圈49，所述混匀盖27的中空结构可供加液针26穿过，所述弹簧48安装在混匀盖27与固定板14之间，所述混匀盖27穿过固定板14后安装轴用弹性挡圈49，所述轴用弹性挡圈49的外径大于固定板14上混匀盖27的安装孔，所述混匀盖27底部设置底部凹槽271，所述底部凹槽271设置为截面为等腰梯形的锥台。
所述底板1上安装感应器架36，所述感应器架36上安装混匀光电开关35、电机监测光电开关44和零位感应光电开关45，所述混匀座29上安装与混匀光电开关35位置对应的混匀感应片47，所述清洗盘座38上安装与电机监测光电开关44位置对应的码盘10，所述清洗盘座38上还安装与零位感应光电开关45位置对应的零位感应片43，通过设置混匀感应片47和混匀光电开关35，用以监测混匀座29的运行情况，防止发生混匀座29卡死的现象发生；通过设置电机监测光电开关44和码盘10，用以监测清洗盘座38的运行情况，防止发生清洗盘座38卡死的现象发生；通过设置零位感应光电开关45和零位感应片43，用以检测系统零位。
所述加液针26、首次加液针58和预激发液加液针59上均安装接头21，所述马达支架19上安装可放置若干个接头21的接头支架22，通过设置接头21，便于维护和更换加液针26，降低维护成本。
通过设置转轴模组驱动清洗盘13定时旋转以及磁珠吸附模组、吸液模组和加液模 组，实现对清洗盘13上的反应杯12进行磁吸、吸液、加液、混匀、加预激发液操作，且实现对清洗盘13的不同孔位同时进行上述操作，从而实现清洗盘13旋转一周即可完成整个清洗过程，提高清洗效率；通过设置侧面安装有若干个磁铁28的磁吸盘11，实现将反应杯12内的磁珠吸附到反应杯12的侧面，然后进行吸液操作，可以避免现有技术中将磁珠吸附到反应杯12底面而造成吸液时误吸磁珠，避免影响分析数据的准确性；通过设置混匀模组，通过借助混匀升降电机40实现混匀承托单元对反应杯12进行原位顶起，无需将反应杯12取出且同时可以进行加液操作，操作步骤紧凑，节省清洗时间，节约设备成本，通过混匀压紧单元对顶起的反应杯12进行压紧限位，从而保证混匀操作时反应杯12的稳定性，防止反应杯12混匀时发生外溅现象；通过将混匀承托单元中的混匀托头50设置为偏心旋转安装在混匀轴55上部，在混匀震荡电机39的作用下驱动混匀从动轮46带动混匀轴55旋转，从而驱动混匀托头50沿混匀轴55偏心旋转运动，从而实现反应杯12顶部压紧限位，底部作旋转运动的动作，该种偏心震荡混匀的方式将磁珠与清洗液进行充分混匀，混匀效果好，混匀时间短，混匀成本低。
工作原理：将反应杯12放置于放杯位，转轴电机4旋转，通过转轴主带轮8驱动转轴从带轮9旋转，与转轴从带轮9固定安装的清洗盘座38旋转带动清洗盘13顺时针旋转，当反应杯12到达首次加液位132位置时，加液；加液完成后清洗盘13继续转动，经过第一组四个磁吸位133进行磁吸操作，然后到达第一个磁吸吸液位134，升降马达20动作，驱动吸液针架16下降，吸液针25深入反应杯12内，磁吸的同时进行吸液操作，下一步进入第一个加液混匀位135，混匀升降电机40动作使混匀座29上升，从而使混匀托头50上升将反应杯12托起，加液针26将清洗液注入反应杯后，混匀震荡电机39动作，通过混匀主带轮51和混匀从带轮46驱动混匀轴55旋转，从而驱动混匀托头50做偏心旋转运动，从而完成反应杯12的偏心震荡混匀动作，下一步进入第二组五个磁吸位136进行磁吸操作，然后到达第二个磁吸吸液位137进行磁吸同时吸液的动作，依次旋转经过第二个加液混匀位138、第三组五个磁吸位139、第三个磁吸吸液位1310、第四个加液混匀位1311、第四组五个磁吸位1312、第四个磁吸吸液位1313、加预激发液混匀位1314进行相应的操作，最后反应杯12到达取杯位，清洗过程完成。清洗盘每转动一个孔位，所有杯位的动作都是同时进行，因此实现不停的放杯、加液、磁吸、吸液、加液混匀、取杯等操作。
以上所述仅为本发明的较佳实施例，并不用以限制本发明，凡在本发明的精神和原 则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。

Claims (10)

  1. 一种全自动磁珠清洗装置，其特征在于：包括底板、用以放置若干个反应杯的清洗盘、用以驱动清洗盘定时旋转的转轴模组、用以对反应杯内的磁珠进行吸附的磁珠吸附模组、对反应杯进行吸液的吸液模组、对反应杯进行加液的加液模组和用以对反应杯进行混匀操作的混匀模组；所述转轴模组包括固定安装在底板上的中空轴和转动安装且同心安装在中空轴上的清洗盘座，所述清洗盘同心固定安装在清洗盘座上；
    所述磁珠吸附模组包括侧面安装有若干个磁铁的磁吸盘，所述磁吸盘的底部通过若干个立柱固定安装在底板上且位于清洗盘的正下方，所述磁吸盘中心设置可供清洗盘座穿过的通孔；
    所述吸液模组包括固定安装在中空轴顶部的固定板、相对于固定板升降安装的吸液针架和安装在吸液针架上且穿过固定板的吸液单元；
    所述加液模组包括安装在固定板上的加液单元以及固定在吸液针架上的加液机构；
    所述混匀模组包括滑动安装在立柱上的混匀座、混匀震荡电机、混匀升降电机、若干个安装在混匀座上的混匀承托单元和若干个安装在固定板上的混匀压紧单元，所述混匀升降电机固定安装在底板上，所述混匀升降电机的混匀升降电机杆固定安装在混匀座上，所述混匀震荡电机固定安装在混匀座的底面上，所述混匀震荡电机的电机杆端部安装混匀主带轮，所述混匀主带轮通过混匀同步带与若干个混匀从带轮连接，所述混匀承托单元与混匀压紧单元在竖直方向上同轴心；
    所述混匀承托单元包括若干个固定安装在混匀座上的轴承座和通过轴承同心安装在轴承座上的混匀轴，所述混匀轴与混匀从带轮同轴固定安装，所述混匀托头通过轴承偏心旋转安装在混匀轴上部，所述混匀托头侧面安装防转板，所述轴承座上垂直安装防转杆，所述防转板上设置可供防转杆穿透的长条孔，所述混匀托头顶部设置顶部凹槽，所述顶部凹槽设置为圆弧面；
    所述混匀压紧单元包括截面为T型且中空的混匀盖、套装在混匀盖上的弹簧和轴用弹簧挡圈，所述混匀盖的中空结构可供加液针穿过，所述弹簧安装在混匀盖与固定板之间，所述混匀盖穿过固定板后安装轴用弹性挡圈，所述轴用弹性挡圈的外径大于固定板上混匀盖的安装孔，所述混匀盖底部设置底部凹槽，所述底部凹槽设置为截面为等腰梯形的锥台。
  2. 根据权利要求1所述的一种全自动磁珠清洗装置，其特征在于：所述转轴模组还包括动力旋转机构，所述动力旋转机构包括通过转轴电机支座安装在底板上的转轴电机、安装在转轴电机杆上的转轴主带轮和与转轴主带轮通过转轴同步带连接的转轴从带轮， 所述转轴从带轮与清洗盘座同心固定安装。
  3. 根据权利要求1或2所述的一种全自动磁珠清洗装置，其特征在于：所述清洗盘上设置三十六个用于放置反应杯的杯位，其中包括顺时针依次圆周排列的八个取放杯位、一个首次加液位、第一组四个磁吸位、第一个磁吸吸液位、第一个加液混匀位、第二组五个磁吸位、第二个磁吸吸液位、第二个加液混匀位、第三组五个磁吸位、第三个磁吸吸液位、第四个加液混匀位、第四组五个磁吸位、第四个磁吸吸液位和一个加预激发液混匀位。
  4. 根据权利要求1或2所述的一种全自动磁珠清洗装置，其特征在于：所述吸液模组还包括通过马达支架安装在固定板上的升降马达，所述升降马达的升降马达杆与吸液针架固定安装，所述吸液针架与固定板之间还设置若干个相对升降滑动安装的导向杆和导向套，所述导向套固定安装在吸液针架上，所述导向杆的一端固定在固定板上，另一端穿过导向套。
  5. 根据权利要求4所述的一种全自动磁珠清洗装置，其特征在于：所述吸液单元包括底部螺纹安装在吸液针架上的等高螺钉、套装在等高螺钉上的弹簧、安装在弹簧和吸液针架之间的缓冲板及穿透且固定在缓冲板上的吸液针，所述吸液针穿透吸液针架。
  6. 根据权利要求1或2所述的一种全自动磁珠清洗装置，其特征在于：所述加液单元包括安装在固定板上的加液针架和穿过加液针架并进行固定的加液针，所述加液针穿过混匀盖，所述加液机构包括设置在吸液针架上的首次加液针和预激发液加液针。
  7. 根据权利要求1所述的一种全自动磁珠清洗装置，其特征在于：所述底板上安装感应器架，所述感应器架上安装混匀光电开关、电机监测光电开关和零位感应光电开关，所述混匀座上安装与混匀光电开关位置对应的混匀感应片，所述清洗盘座上安装与电机监测光电开关位置对应的码盘，所述清洗盘座上还安装与零位感应光电开关位置对应的零位感应片。
  8. 根据权利要求1所述的一种全自动磁珠清洗装置，其特征在于：所述混匀座底面安装若干个位置可调的张紧轮，所述张紧轮上安装混匀同步带。
  9. 根据权利要求2所述的一种全自动磁珠清洗装置，其特征在于：所述转轴电机上还设置张紧板，所述张紧板两端固定安装在转轴电机支座上，所述张紧板上安装顶紧螺钉，所述顶紧螺钉的一端顶紧转轴电机，另一端螺纹安装在张紧板上。
  10. 根据权利要求6所述的一种全自动磁珠清洗装置，其特征在于：所述加液针、首次加液针和预激发液加液针上均安装接头，所述马达支架上安装可放置若干个接头的接 头支架。
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