WO2020253233A1 - 一种禽用植物精油组合物及其制备方法和应用 - Google Patents
一种禽用植物精油组合物及其制备方法和应用 Download PDFInfo
- Publication number
- WO2020253233A1 WO2020253233A1 PCT/CN2020/071627 CN2020071627W WO2020253233A1 WO 2020253233 A1 WO2020253233 A1 WO 2020253233A1 CN 2020071627 W CN2020071627 W CN 2020071627W WO 2020253233 A1 WO2020253233 A1 WO 2020253233A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- poultry
- essential oil
- parts
- plant essential
- oil composition
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
- A61K31/05—Phenols
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/61—Myrtaceae (Myrtle family), e.g. teatree or eucalyptus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
Definitions
- the invention relates to a plant essential oil composition for poultry, a preparation method and application thereof, and belongs to the field of poultry medicine.
- Chicken coccidiosis is a parasitic disease with a very high incidence and fatality rate in chickens. It is a global epidemic that seriously harms the chicken industry. It has a huge economic impact on farmers every year. It affects the development of the entire poultry industry and poses a major threat. At present, there are many types of chicken coccidiosis, and there are 7 recognized in the world. Among them, Eimeria necatrix and Eimeria tenella are the most serious.
- antibiotics to prevent and treat chicken coccidiosis, including polyether carrier antibiotics and chemically synthesized anticoccidial drugs, such as salinomycin, maduramycin, diclazuril, totrezuril, Gram ball powder, Nicarbazine, Amproline and other drugs.
- polyether carrier antibiotics such as polyether carrier antibiotics and chemically synthesized anticoccidial drugs, such as salinomycin, maduramycin, diclazuril, totrezuril, Gram ball powder, Nicarbazine, Amproline and other drugs.
- anticoccidial drugs such as salinomycin, maduramycin, diclazuril, totrezuril, Gram ball powder, Nicarbazine, Amproline and other drugs.
- the present invention provides a plant essential oil composition for poultry and its preparation Methods and applications.
- a plant essential oil composition for poultry consisting of the following raw materials in parts by weight:
- a plant essential oil composition composed of plant essential oils carvacrol, thymol and eucalyptus oil in a specific ratio has a significant therapeutic effect on poultry coccidiosis, and long-term use will not produce resistance
- the present invention can also be improved as follows.
- the poultry plant essential oil composition consists of the following raw materials in parts by weight:
- the poultry plant essential oil composition consists of the following raw materials in parts by weight:
- the above two groups of poultry plant essential oil compositions, the carvacrol, thymol and eucalyptus oil used have different degrees of inhibition on most gram-positive bacteria and gram-negative bacteria, and have strong antibacterial ability.
- the membrane structure of bacterial cells changes its permeability to achieve the effect of killing bacteria.
- Carvacrol and thymol act on the body's intestinal tract, play a beneficial role in the intestinal tract, improve production performance and body immunity, and relieve necrotizing enteritis.
- Eucalyptus oil has a broad-spectrum insecticidal effect and is one of the research directions of natural insecticides. Eimeria is a parasite, and eucalyptus oil has an inhibitory effect on it.
- the combination of the three plant essential oils can treat and prevent the occurrence of coccidiosis, at the same time make the body's intestines healthy, and ultimately improve the performance of poultry and the body's immunity.
- the present invention also provides a method for preparing the above-mentioned poultry essential oil composition, which comprises:
- the present invention can also be improved as follows.
- the adsorbent includes any one or a mixture of white carbon black, cyclodextrins, modified starch and porous starch.
- the beneficial effect of adopting the above-mentioned further scheme is that the above-mentioned adsorbent can uniformly adsorb plant essential oils and effectively avoid the volatilization of carvacrol, thymol and eucalyptus oil.
- the coating material is any one of stearic acid and its salts, and enteric sustained-release packaging materials.
- the stearic acid and its salts are preferably any one of C16-C18 fatty acids, glyceryl monostearate, hydrogenated fatty acids and fatty alcohols.
- the enteric sustained-release packaging material is preferably any one of polypropylene resin IV and chitosan.
- the coating adopts stearic acid or enteric-soluble sustained-release packaging material, and uses its hydrophilic and lipophilic properties to achieve emulsification and solubilization effects, so that carvacrol, thymol and eucalyptus oil can be effective It plays a role in the intestinal tract, increasing the height of small intestinal villi, reducing the depth of crypts, and at the same time increasing the activity of intestinal digestive enzymes, thereby improving the digestion, absorption and transport of nutrients in the intestine. At the same time, it can also reduce the volatility of essential oils, improve palatability, and ensure the uniformity and stability of the product.
- the plant essential oil composition for poultry when it is not coated, it also includes adsorbing the plant essential oil composition for poultry with an organic carrier adsorbent or an inorganic carrier adsorbent.
- the above-mentioned inorganic carrier adsorbent is preferably any one or a mixture of several of starch, magnesium oxide, calcium hydrogen phosphate and silicate.
- the beneficial effect of adopting the above-mentioned further scheme is that the plant essential oil is absorbed into the above-mentioned carrier and the volatility of the plant essential oil is reduced.
- the present invention also provides a medicine for preventing and treating avian coccidiosis, including the above-mentioned avian plant essential oil composition.
- the poultry plant essential oil composition of the present invention When using the poultry plant essential oil composition of the present invention to prevent and treat poultry coccidiosis, it is added to the poultry compound feed or drinking water, and 10-400 grams of the poultry plant essential oil composition is added per ton of poultry compound feed, preferably 50-300 grams; or add 5-250 grams of the above-mentioned poultry essential oil composition per ton of drinking water, preferably 25-200 grams.
- the three plant essential oil mixtures are volatile and easily oxidized. They should be sealed in an aluminum foil bag, stored below 25°C, and unpacked as needed.
- test chicks were male chicks of Hailan gray layer. They were fed the basic diet at the age of 0 days. They were grouped from the age of 12 days and were randomly divided into eight groups, each with 3 replicates, and each replicate with 30 chickens. among them,
- the first group is the negative control group, fed with basal diet + 1000 grams of zeolite powder;
- the second group was fed basal diet + Baiqiuqing 0.5ml/L;
- the third group was fed a basal diet + 12.5% carvacrol and thymol (carvacrol 5% + thymol 7.5%/carvacrol 8% + thymol 4.5%) composition 2000 g/ton, this essential oil combination
- the preparation method of the substance is the same as in Example 1;
- the fourth group was fed with basal diet + 5% eucalyptus oil 2000 g/ton, and the preparation method of this essential oil composition was the same as the embodiment;
- the fifth group was fed with basal diet + 500 g/ton of the composition of Example 1;
- the sixth group was fed with basal diet + 1000 g/ton of the composition of Example 1;
- the seventh group was fed with basal diet + 2000 g/ton of the composition of Example 1;
- the eighth group was the positive control group, fed with basal diet + 1,000 grams of zeolite powder.
- the second-eighth group was infected with 70,000 spores of Eimeria tenella at the age of 14 days, and the experiment ended at the age of 22 days.
- the basic diet formula is: corn 65wt%, soybean meal 31wt%, stone powder 1.6wt%, calcium hydrogen phosphate 1.1wt%, premix 0.5wt%, sodium chloride 0.3wt%, lysine 0.22wt%, methionine 0.18wt% % And 0.1wt% of choline chloride.
- the nutrients in the above basic diet formula are: metabolizable energy 2823 kcal/kg feed, crude protein 18.5% by weight, calcium 0.95% by weight, phosphorus 0.37% by weight, salt 0.29% by weight, lysine 1.1% by weight, methionine + cysteine Acid 0.8wt%.
- the chicks eat freely, drink freely, and follow the management of laying hens.
- Survival rate the ratio of the number of survivals in the test group to the number of survivals in the non-infection and non-administered (blank control) group (%)
- Relative weight gain rate the weight gain of the test group accounts for the weight gain of the non-infected and non-administered (blank control) group (%)
- Lesion value average intestinal lesion score of each test group (0-4)*10
- Lesion scoring method Eimeria tenella infection, when the cecum lesions on both sides of broilers are inconsistent, the severe side shall prevail.
- Oocyst production stool 5-8 days after infection with coccidia and oocysts in the cecum on day 8
- Oocyst ratio ratio of oocyst output in the drug group to the oocyst output in the non-infected (infected control) group (%)
- the oocyst value is 0, 5, 10, respectively , 20 and 40.
- ACI 160-179, judged as partly resistant (partly sensitive or partly effective),
- ACI (survival rate + relative weight gain rate)-(lesion value + oocyst value)
- the relative weight gain index indicates that the relative weight gain rate of group 7 reaches 99.05%, indicating that 2000g/t of the combination of Example 1 was added after coccidia infection
- the relative weight gain rate of group 3 was the lowest, indicating that the effect of carvacrol and thymol composition was significantly lower than that of the three essential oil compositions.
- the relative weight gain of group 4 eucalyptus oil group Although the rate is higher than that of group 3, the effect is still not up to the three essential oil compositions; the survival rate index shows that no chickens died in each group, which may be related to the short test period; the disease score index shows that the disease of group 4 least.
- the anti-coccidial index ACI is obtained by combining various indicators, indicating that the anti-coccidial effect of the group 3 and group 5 doses is invalid, the anti-coccidial effect of the group 4 and group 6 doses are partially effective, and the anti-coccidial effect of the group 7 doses is effective , To achieve the same effect as the antibiotic group. Therefore, it is shown that adding 2000g/t of the composition of Example 1 to the diet can achieve the effect of replacing group 2 antibiotics, and can prevent and treat chicken anticoccidial diseases.
- Example 1 the effect of the composition of Example 1 is significantly better than the combination effect of carvacrol and thymol composition, and also better than the effect of the eucalyptus oil group, indicating that the three plant essential oil compositions are significantly better than the effect of using alone.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Veterinary Medicine (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Epidemiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Medicines Containing Plant Substances (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fodder In General (AREA)
Abstract
一种禽用植物精油组合物及其制备方法和应用。该禽用植物精油组合物由如下重量份的原料组成：香芹酚0-10份、百里香酚0-10份和桉叶油1-10份。该植物精油组合物对禽类球虫病有明显的治疗效果。
Description
本发明涉及一种禽用植物精油组合物及其制备方法和应用，属于禽类药物领域。
鸡球虫病是一种在鸡上发病率和致死率都非常高的寄生虫病，是一种全球性流行的严重危害养鸡产业的重大疾病，每年给养殖户产生巨大的经济影响数以亿计，影响整个养禽行业的发展，造成重大威胁。目前造成鸡球虫病的种类众多，世界公认的有7种，其中毒害艾美耳球虫(Eimeria necatrix)和柔嫩艾美耳球虫(Eimeria tenella)的危害最为严重。
目前我国主要采用抗菌素来预防和治疗鸡球虫病的发生，包括聚醚类载体抗生素和化学合成抗球虫药，如盐霉素、马杜霉素、地克珠利、妥曲珠利、克球粉、尼卡巴嗪、氨丙啉等药物。但是，长期使用抗生素会导致耐药性、药物残留、毒副作用强，且治疗费用支出高等问题。加之，人们现在已经意识到食品安全问题，且农业农村部已出台相关方案，力争通过3年时间，实施养殖环节兽用抗菌药使用减量化行动试点工作，推广兽用抗菌药使用减量化模式，减少使用抗菌药类药物饲料添加剂，兽用抗菌药使用量实现“零增长”，兽药残留和动物细菌耐药问题得到有效控制。
因此，寻求新型的替代产品成为大势所趋。例如，开发中草药(如青蒿、野菊花、常山、柴胡等)、微生态制剂(如乳酸菌)、强毒或弱毒疫苗、植物提取物(如鸦胆子、金丝桃等)。而植物精油作为植物提取物中的一种，鲜有报道。
发明内容
本发明为了解决现有技术长期使用抗生素来预防和治疗禽类球虫病，导致的耐药性、药物残留、毒副作用强和治疗费用高等技术问题，提供了一种禽用植物精油组合物及其制备方法和应用。
本发明解决上述技术问题的技术方案如下：一种禽用植物精油组合物，由如下重量份的原料组成：
香芹酚0-10份、百里香酚0-10份和桉叶油1-10份。
本发明禽用植物精油组合物的有益效果是：
本发明人经多年研究意外发现，将植物精油香芹酚、百里香酚和桉叶油按特定比例组成的植物精油组合物，对禽类球虫病有明显的治疗效果，且长期使用不会产生耐药性、药物残留和毒副作用，治疗费用低，能够作为抗菌素的替代产品在畜牧业生产中发挥重要作用。
在上述技术方案的基础上，本发明还可以做如下改进。
进一步，所述禽用植物精油组合物，由如下重量份的原料组成：
香芹酚3份、百里香酚8份和桉叶油4份。
进一步，所述禽用植物精油组合物，由如下重量份的原料组成：
香芹酚7份、百里香酚3份和桉叶油5份。
上述两组禽用植物精油组合物，所用香芹酚、百里香酚和桉叶油对大多数革兰氏阳性菌和革兰氏阴性菌都有不同程度的抑制作用，抗菌能力很强，作用于细菌细胞的膜结构，改变其通透性，达到杀灭细菌的效果。香芹酚、百里香酚作用于机体肠道，在肠道发挥有益作用，提高生产性能和机体免疫力，缓解坏死性肠炎。而桉叶油具有广谱杀虫效果，是天然杀虫剂研究的方向之一。艾美耳球虫作为一种寄生虫，桉叶油在其方面有抑制作用。三种植物精油组合搭配可达到治疗和预防球虫病的发生，同时使机体肠道健康，最终提高禽类生产性能和机体免疫力。
本发明还提供一种如上所述的禽用植物精油组合物的制备方法，包括：
1)取百里香酚，加热到85℃以上熔融，再加入香芹酚和桉叶油，混合均匀，得混合精油；
2)向1)所得混合精油中加入吸附剂吸附至饱和，包衣或不包衣，即得禽用植物精油组合物。
在上述技术方案的基础上，本发明还可以做如下改进。
进一步，在2)中，所述吸附剂包括白炭黑、环糊精类、改性淀粉和多孔淀粉中的任意一种或几种的混合物。
采用上述进一步方案的有益效果是：上述吸附剂能均匀的吸附植物精油，有效避免香芹酚、百里香酚和桉叶油的挥发。
进一步，在2)中，所述包衣材料为硬脂酸及其盐类和肠溶性缓释包材中的任意一种。上述硬脂酸及其盐类优选为C16-C18脂肪酸、单硬脂酸甘油酯、氢化脂肪酸和脂肪醇中的任意一种。上述肠溶性缓释包材优选为聚丙烯树脂IV和壳聚糖中的任意一种。
采用上述进一步方案的有益效果是：包衣采用硬脂酸或肠溶性缓释包材，利用其亲水亲油特性，达到乳化增溶效果，使香芹酚、百里香酚和桉叶油能有效地在肠道中发挥作用，提高肠道小肠绒毛高度，降低隐窝深度，同时提高肠道消化酶活性，进而起到提高肠道对营养物质的消化、吸收和转运。同时，还可以降低植物精油的挥发性，提高适口性，保证产品的混合均匀度和稳定性。
进一步，在2)中，还包括当不包衣时，用有机载体吸附剂或无机载体吸附剂吸附禽用植物精油组合物。上述无机载体吸附剂优选为淀粉类、氧化镁、磷酸氢钙和硅酸盐类中的任意一种或几种的混合物。
采用上述进一步方案的有益效果是：使植物精油吸附到上述载体中，同时降低植物精油的挥发性。
本发明还提供一种预防和治疗禽类球虫病的药物，包括上述禽用植物精 油组合物。
使用本发明禽用植物精油组合物预防和治疗禽类球虫病时，将其添加到禽配合饲料或饮水中，每吨禽配合饲料中添加上述禽用植物精油组合物10-400克，优选为50-300克；或在每吨饮水中添加上述禽用植物精油组合物5-250克，优选为25-200克。
以下对本发明的原理和特征进行描述，所举实例只用于解释本发明，并非用于限定本发明的范围。
实施例1
1)分别称取香芹酚3g、百里香酚8g和桉叶油4g。
2)将百里香酚加热到90℃熔融，加入香芹酚和桉叶油，混合均匀，再加入7g白炭黑吸附至饱和，得到三种植物精油混合物。
3)称取单硬脂酸甘油酯78g，加热到90℃熔融，加入2)所得三种植物精油混合物，通过离心喷雾干燥机的喷头喷到冷却塔中，即得包衣的禽用植物精油组合物。
或，称取单硬脂酸甘油酯78g,加热到90℃熔融，把2)所得三种植物精油混合物加到包衣机的流化床中流化，把上述熔融的单硬脂酸甘油酯喷涂到三种植物精油混合物的表面，即得包衣的禽用植物精油组合物。
实施例2
1)分别称取香芹酚9g和桉叶油6g。
2)将香芹酚和桉叶油混合均匀，再加入7g白炭黑吸附至饱和，得到两种植物精油混合物。
3)称取单硬脂酸甘油酯78g，加热到90℃熔融，加入2)所得两种植物精油混合物，通过离心喷雾干燥机的喷头喷到冷却塔中，即得包衣的禽用植 物精油组合物。
或，称取单硬脂酸甘油酯78g，加热到90℃熔融，把2)所得两种植物精油混合物加到包衣机的流化床中流化，把上述熔融的单硬脂酸甘油酯喷涂到两种植物精油混合物的表面，即得包衣的禽用植物精油组合物。
实施例3
1)分别称取百里香酚10g和桉叶油4g。
2)将百里香酚加热到90℃熔融，加入桉叶油，混合均匀，再加入7g白炭黑吸附至饱和，得到两种植物精油混合物。
3)称取单硬脂酸甘油酯78g，加热到90℃熔融，加入2)所得两种植物精油混合物，通过离心喷雾干燥机的喷头喷到冷却塔中，即得包衣的禽用植物精油组合物。
或，称取单硬脂酸甘油酯78g，加热到90℃熔融，把2)所得两种植物精油混合物加到包衣机的流化床中流化，把熔融的单硬脂酸甘油酯喷涂到两种植物精油混合物的表面，即得包衣的禽用植物精油组合物。
实施例4
1)分别称取香芹酚7g、百里香酚3g和桉叶油5g。
2)将百里香酚加热到90℃熔融，加入香芹酚和桉叶油，混合均匀，再用85g多孔淀粉吸附至饱和，得到三种植物精油混合物。
此时三种植物精油混合物易挥发、易氧化，应密封到铝箔袋中，25℃以下保存，随用随开包装。
测定效果实验
1、试验材料和试验设计
供试雏鸡，品种为海兰灰蛋鸡公雏，0日龄开始饲喂基础日粮，从12 日龄开始分组，随机分为八组，每组3个重复，每个重复30只鸡。其中，
第一组为阴性对照组，饲喂基础日粮+沸石粉1000克；
第二组饲喂基础日粮+百球清0.5ml/L；
第三组饲喂基础日粮+12.5％香芹酚和百里香酚(香芹酚5％+百里香酚7.5％/香芹酚8％+百里香酚4.5％)组合物2000克/吨，此精油组合物的制备方法同实施例1；
第四组饲喂基础日粮+5％桉叶油2000克/吨，此精油组合物的制备方法同实施例；
第五组饲喂基础日粮+实施例1组合物500克/吨；
第六组饲喂基础日粮+实施例1组合物1000克/吨；
第七组饲喂基础日粮+实施例1组合物2000克/吨；
第八组为阳性对照组，饲喂基础日粮+沸石粉1000克。
第二组-第八组在14日龄时感染柔嫩艾美耳球虫的孢子化乱囊7万，试验22日龄结束。
本试验由第三方北京农学院安健教授完成，安健教授一直从事鸡球虫病研究20余年，试验为盲试。
2、试验日粮及饲养管理
基础日粮配方为：玉米65wt％、豆粕31wt％、石粉1.6wt％、磷酸氢钙1.1wt％、预混料0.5wt％、氯化钠0.3wt％、赖氨酸0.22wt％、蛋氨酸0.18wt％和氯化胆碱0.1wt％。
上述基础日粮配方中营养成分为：代谢能2823千卡/千克饲料，粗蛋白18.5wt％，钙0.95wt％，磷0.37wt％，盐0.29wt％，赖氨酸1.1wt％，蛋氨酸+胱氨酸0.8wt％。
雏鸡自由采食，自由饮水，按照蛋鸡饲养管理进行。
3、试验测定指标
3.1存活率
存活率＝试验组存活数占不感染不给药(空白对照)组存活数的比例(％)
3.2相对增重率
相对增重率＝试验组增重占不感染不给药(空白对照)组增重的比例(％)
3.3病变值
病变值＝各试验组平均肠道病变记分(0-4)*10
病变记分方法：柔嫩艾美耳球虫感染，肉鸡两侧盲肠病变不一致时，以严重的一侧为准。
0分，无肉眼可见病变。
+1分，盲肠壁有很少量散在的出血点，肠壁不增厚，内容物正常。
+2分，病变数量较多，盲肠内容物明显带血，盲肠壁稍增厚，内容物正常。
+3分，盲肠内有多量血液或有盲肠芯，肠芯为血凝块或灰白色或红白相间的干酪样的块状物，肠壁肥厚明显，盲肠中粪便含量少。
+4分，因充满大量血液或肠芯而盲肠肿大，肠芯中多含有粪渣，或者肠道极度萎缩，肠壁极度肥厚；死亡鸡只也记+4分。
3.4卵囊值
卵囊产量＝感染球虫后5-8天粪便和第8天盲肠中卵囊
卵囊比数＝药物组卵囊产量占感染不给药(感染对照)组卵囊产量的比例(％)
卵囊比数小于1.0(％)、1-25(％)、26-50(％)、51-75(％)、76-≥100(％)的，卵囊值分别为0、5、10、20和40。
当ACI≥180，判为敏感(有效)，
ACI＝160-179，判为部分抗药(部分敏感或部分有效)，
ACI<160，判为抗药(无效)。
3.5抗球虫指数(ACI)
ACI＝(存活率+相对增重率)-(病变值+卵囊值)
4、数据记录及分析
12日龄称取每组鸡只重量，试验22日龄结束后称取鸡只重量，计算每组平均值进行数据分析，判定各组植物精油组合物的敏感性。
5、结果与分析
表1试验结果
从表1可以得出，以组1(阴性对照组)为基准，从相对增重率指标表明，组7的相对增重率达到99.05％，说明感染球虫后添加2000g/t实施例1组合物达到未感染球虫效果，组3的相对增重率最低，表明香芹酚和百里香酚组合物的效果明显低于三种精油组合物搭配的效果，组4桉叶油组的相对增重率虽比组3高，但效果仍未达到三种精油组合物；从存活率指标表明，各组均无鸡只死亡，可能与试验周期短有关；从病变分值指标表明，组4的病变最少。
综合各项指标，得出抗球虫指数ACI，表明组3和组5剂量的抗球虫作用无效，组4和组6剂量的抗球虫作用部分有效，组7剂量的抗球虫作用有效，达到与抗菌素组同样效果。因此说明，在日粮中添加2000g/t的实施例1组合物可以达到替代组2抗菌素效果，可以预防和治疗鸡的抗球虫疾病。同时，实施例1组合物效果明显优于香芹酚和百里香酚组合物搭配效果，也优于桉叶油组的效果，说明三种植物精油组合物明显优于单独使用效果。
以上所述仅为本发明的较佳实施例，并不用以限制本发明，凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。
Claims (10)
- 一种禽用植物精油组合物，其特征在于，由如下重量份的原料组成：香芹酚0-10份、百里香酚0-10份和桉叶油1-10份。
- 根据权利要求1所述的禽用植物精油组合物，其特征在于，由如下重量份的原料组成：香芹酚3份、百里香酚8份和桉叶油4份。
- 根据权利要求1所述的禽用植物精油组合物，其特征在于，由如下重量份的原料组成：香芹酚7份、百里香酚3份和桉叶油5份。
- 一种如权利要求1-3任一项所述的禽用植物精油组合物的制备方法，其特征在于，包括：1)取百里香酚，加热到85℃以上熔融，再加入香芹酚和桉叶油，混合均匀，得混合精油；2)向1)所得混合精油中加入吸附剂吸附至饱和，包衣或不包衣，即得禽用植物精油组合物。
- 根据权利要求4所述的制备方法，其特征在于，在2)中，所述吸附剂包括白炭黑、环糊精类、改性淀粉和多孔淀粉中的任意一种或几种的混合物。
- 根据权利要求4所述的制备方法，其特征在于，在2)中，所述包衣材料为硬脂酸及其盐类和肠溶性缓释包材中的任意一种。
- 根据权利要求6所述的制备方法，其特征在于，所述硬脂酸及其盐类包括C16-C18脂肪酸、单硬脂酸甘油酯、氢化脂肪酸和脂肪醇中的任意一种，所述肠溶性缓释包材包括聚丙烯树脂IV和壳聚糖中的任意一种。
- 根据权利要求4所述的制备方法，其特征在于，在2)中，还包括当不包衣时，用有机载体吸附剂或无机载体吸附剂吸附禽用植物精油组合物。
- 根据权利要求8所述的制备方法，其特征在于，所述无机载体吸附剂包括淀粉类、氧化镁、磷酸氢钙和硅酸盐类中的任意一种或几种的混合物。
- 一种预防和治疗禽类球虫病的药物，其特征在于，包括权利要求1-3任一项所述的禽用植物精油组合物。
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910543001.3 | 2019-06-21 | ||
| CN201910543001.3A CN110201035B (zh) | 2019-06-21 | 2019-06-21 | 一种禽用植物精油组合物及其制备方法和应用 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2020253233A1 true WO2020253233A1 (zh) | 2020-12-24 |
Family
ID=67793959
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/CN2020/071627 WO2020253233A1 (zh) | 2019-06-21 | 2020-01-13 | 一种禽用植物精油组合物及其制备方法和应用 |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN110201035B (zh) |
| WO (1) | WO2020253233A1 (zh) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110201035B (zh) * | 2019-06-21 | 2021-08-24 | 辽宁菲迪饲料科技有限责任公司 | 一种禽用植物精油组合物及其制备方法和应用 |
| CN112655822A (zh) * | 2020-12-07 | 2021-04-16 | 辽宁菲迪饲料科技有限责任公司 | 一种畜禽用饲料添加剂、其制备方法及应用 |
| CN113134031A (zh) * | 2021-05-17 | 2021-07-20 | 广东瑞生科技集团有限公司 | 一种防治球虫的植物精油及其用途 |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105475666A (zh) * | 2015-12-07 | 2016-04-13 | 山川生物科技(武汉)有限公司 | 一种防治鸡球虫病的饲料添加剂制备方法 |
| CN108186736A (zh) * | 2018-01-17 | 2018-06-22 | 珠海天翼医药技术开发有限公司 | 一种自乳化精油及其制备方法和应用 |
| CN108991243A (zh) * | 2018-06-25 | 2018-12-14 | 济南牧德生物科技有限公司 | 一种固体水溶性复合精油及其制备方法 |
| CN109601722A (zh) * | 2018-12-17 | 2019-04-12 | 广东有机宝生物科技股份有限公司 | 一种月桂酸单甘油酯包被植物精油的制备方法 |
| CN110201035A (zh) * | 2019-06-21 | 2019-09-06 | 刘春海 | 一种禽用植物精油组合物及其制备方法和应用 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107006694B (zh) * | 2017-03-20 | 2019-03-01 | 刘春海 | 一种肉牛、肉羊用植物精油组合物、制备方法及其应用 |
| CN107373088A (zh) * | 2017-08-25 | 2017-11-24 | 福建傲农生物科技集团股份有限公司 | 一种替代仔猪饲料抗生素的植物精油组合物及其制备方法与应用 |
-
2019
- 2019-06-21 CN CN201910543001.3A patent/CN110201035B/zh active Active
-
2020
- 2020-01-13 WO PCT/CN2020/071627 patent/WO2020253233A1/zh active Application Filing
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105475666A (zh) * | 2015-12-07 | 2016-04-13 | 山川生物科技(武汉)有限公司 | 一种防治鸡球虫病的饲料添加剂制备方法 |
| CN108186736A (zh) * | 2018-01-17 | 2018-06-22 | 珠海天翼医药技术开发有限公司 | 一种自乳化精油及其制备方法和应用 |
| CN108991243A (zh) * | 2018-06-25 | 2018-12-14 | 济南牧德生物科技有限公司 | 一种固体水溶性复合精油及其制备方法 |
| CN109601722A (zh) * | 2018-12-17 | 2019-04-12 | 广东有机宝生物科技股份有限公司 | 一种月桂酸单甘油酯包被植物精油的制备方法 |
| CN110201035A (zh) * | 2019-06-21 | 2019-09-06 | 刘春海 | 一种禽用植物精油组合物及其制备方法和应用 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110201035A (zh) | 2019-09-06 |
| CN110201035B (zh) | 2021-08-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2020253233A1 (zh) | 一种禽用植物精油组合物及其制备方法和应用 | |
| Sun et al. | Effects of supplementation of Bacillus subtilis natto Na and N1 strains on rumen development in dairy calves | |
| CA2896698A1 (en) | Clay product and uses thereof | |
| MX2011006273A (es) | Alimento para prevenir y/o tratar enfermedades debido a la bacteria clostridium sp. en ganado y agente anti-clostridium. | |
| CN110354139B (zh) | 一种抗猪流行性腹泻病毒感染的组合物及其应用 | |
| CN111110772A (zh) | 一种防控畜禽寄生虫固态发酵中药添加剂 | |
| CN102988363B (zh) | 一种治疗家禽腺胃炎和坏死性肠炎的药物及其制备方法 | |
| CN109999027B (zh) | 褪黑素的用途 | |
| CN102920771B (zh) | 一种用于治疗家禽球虫病的药物 | |
| CN101317904B (zh) | 乌梅提取物在制备抗畜禽病毒药物中的用途 | |
| CN103083510A (zh) | 一种提高禽免疫功能的中药口服液及其制备方法 | |
| CN109568301A (zh) | 一种没食子酸、大黄酸复合配方制备方法及其应用 | |
| IE62523B1 (en) | Anticoccidial composition | |
| CN102805772B (zh) | 四黄止痢颗粒在制备治疗禽类肠道疾病药物中的应用及其复配的药物组合物 | |
| WO2022241968A1 (zh) | 一种防治球虫的植物精油及其用途 | |
| Bird et al. | Generalized Edema in Chicks Prevented by d, 1-Alpha Tocopherol. | |
| CN101530405B (zh) | 柠檬酸及复方柠檬酸在制药中的应用 | |
| CN109966289B (zh) | 瑞香素在抗鸡球虫病中的应用 | |
| CN106668038A (zh) | 毛柳苷在制备抗柔嫩艾美耳球虫药物中的应用 | |
| KR20110094935A (ko) | 녹차를 유효성분으로 함유하는 조류 인플루엔자에 대한 항바이러스제 | |
| CN105770054A (zh) | 用于防治鸡球虫病的药物组合物、添加剂和饲料 | |
| CN111821322A (zh) | 一种可替代抗生素的禽用微生态口服制剂及其应用 | |
| CN113813368B (zh) | 一种抗菌肽在制备抗草鱼呼肠孤病毒药物中的应用 | |
| NL2030026B1 (en) | Traditional chinese medicine micropowder composition and application thereof in ecological chicken breeding mode | |
| CN102940707B (zh) | 用于防治鸡球虫病的药物组合物和预混料、配合料 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 20826745 Country of ref document: EP Kind code of ref document: A1 |
|
| NENP | Non-entry into the national phase |
Ref country code: DE |
|
| 122 | Ep: pct application non-entry in european phase |
Ref document number: 20826745 Country of ref document: EP Kind code of ref document: A1 |