NL2030026B1

NL2030026B1 - Traditional chinese medicine micropowder composition and application thereof in ecological chicken breeding mode

Info

Publication number: NL2030026B1
Application number: NL2030026A
Authority: NL
Inventors: Ran Zhiping; Guan Quan; Yang Wenhai; Zhou Hua; He Bin; Shao Zhiyong; Chen Xiabing; Lei Zhixin; Wu Lijun; Zhou Qi; Li Ying
Original assignee: Wuhan Acad Agricultural Sciences; Univ Wuhan Tech
Priority date: 2021-12-03
Filing date: 2021-12-03
Publication date: 2023-06-20

Abstract

The present invention provides a traditional Chinese medicine micropowder composition, and belongs to the technical field of veterinary compound traditional Chinese medicines. The traditional Chinese medicine micropowder composition provided in the present 5 invention includes Fructus Arctii, Fructus Cnidii, Dichroa febrifuga Lour., Astragalus membranaceus, wormwood, Sweet Wormwood Herb and Codonopsis pilosula, and can be applied to a microbial fermentation bed for chicken breeding. The present invention further provides a chicken breeding bed including a padding, a 10 traditional Chinese medicine micropowder composition and a mixed microbial strain fermentation broth. The chicken breeding bed can be matched with traditional Chinese medicine bags hanging in chicken houses, and traditional Chinese medicine preparations with different effects or extracts thereof added to feed or drinking 15 water during breeding to form a healthy, safe, efficient and ecological chicken breeding system, so as to ensure the quality safety of chicken products.

Description

P888/NLpd

TRADITIONAL CHINESE MEDICINE MICROPOWDER COMPOSITION AND

APPLICATION THEREOF IN ECOLOGICAL CHICKEN BREEDING MODE

TECHNICAL FIELD

The present invention belongs to the technical field of vet- erinary compound traditional Chinese medicines, and relates to a traditional Chinese medicine micropowder composition and an appli- cation thereof to chicken breeding.

BACKGROUND ART

At present, most of drugs for preventing and treating chicken diseases in the market are antibiotics. These drugs have some tox- ic and side effects and remain in meat and egg products, which will seriously harm the health of people after consumption. There- fore, in the current national environment of reducing, limiting and prohibiting antibiotics, how to effectively develop products that replace antibiotics, reduce the use of chemical drugs such as antibiotics, ensure healthy breeding of livestock and poultry, and reduce drug resistance and residual hazards of drugs such as anti- biotics is an important subject for the livestock and poultry breeding industry at present.

Under the guidance of the theoretical system of traditional

Chinese medicine, how to select common traditional Chinese medici- nal herbs to form a formula preparation to be applied to chicken breeding, and establish an antibiotic-free ecological breeding mode has become the key in the field of chicken breeding.

SUMMARY

The present invention provides a traditional Chinese medicine micropowder composition, including the following raw materials by weight: 3-12 parts of Fructus Arctii, 2-12 parts of Fructus

Cnidii, 1-6 parts of Dichroa febrifuga Lour., 5-16 parts of

Astragalus membranaceus, 5-16 parts of wormwood, 5-12 parts of

Sweet Wormwood Herb and 1-6 parts of Codonopsis pilosula.

In an application of the traditional Chinese medicine mi-

cropowder composition to a fermentation bed for breeding, the fer- mentation bed is a fermentation bed for chicken breeding.

A fermentation bed for chicken breeding, including a padding and 0.05-0.5 wt& of the traditional Chinese medicine micropowder composition.

The fermentation bed further includes a mixed microbial strain fermentation broth.

The mixed microbial strain is prepared from Lactococcus lac- tis LAWH202106, Enterococcus faecalis EFWH202105 and Bacillus li- cheniformis BLHB-20200701 at a ratio of 1-2:1-2:1-2.

In the mixed microbial strain fermentation broth, viable count of the Lactococcus lactis LAWH202106, the Enterococcus fae- calis EFWH202105 and the Bacillus licheniformis BLHB-20200701 is 4-6x10° cfu/ml, 4-6x10% cfu/ml and 4-6x10°% cfu/ml, respectively.

The dosage of the fermentation broth is 0.5-1.6 L/m‘.

A chicken breeding mode, including: breeding chickens in the fermentation bed for chicken breeding, hanging traditional Chinese medicine bags in chicken houses, and adding traditional Chinese medicine preparations or traditional Chinese medicine extracts to feed or drinking water during the chicken breeding.

During breeding of local-breeding laying chickens, 0.5-2% of an Astragalus extract is added on Day 0-7; 0.5-1.0% of a tradi- tional Chinese medicine preparation for preventing and treating chicken diarrhea or an extract thereof is added on Day 8-14; 0.5- 1.0% of a traditional Chinese medicine preparation for preventing and treating avian colibacillosios or an extract thereof is added on Day 15-21 and Day 91-98; 0.5-1.0% of a traditional Chinese med- icine preparation for preventing and treating coccidiosis in chicken or an extract thereof is added on Day 22-27, Day 42-49 and

Day 63-70; and 1-2% of a mixed powder of Astragalus membranaceus, motherwort and Cortex Phellodendri is added on Day 127-133.

During breeding of local-breeding broiler chickens, 0.5-2% of an Astragalus extract is added on Day 0-7; 0.5-1.0% of a tradi- tional Chinese medicine preparation for preventing and treating chicken diarrhea or an extract thereof is added on Day 8-14; 0.5- 1.0% of a traditional Chinese medicine preparation for preventing and treating avian colibacillosios or an extract thereof is added on Day 15-21; 0.5-1.0% of a traditional Chinese medicine prepara- tion for preventing and treating coccidiosis in chicken or an ex- tract thereof is added on Day 22-27 and Day 42-49; and 0.5-2% of an Astragalus extract is added on Day 63-70.

The present invention has the following beneficial effects:

According to the present invention, the traditional Chinese medicine micropowder composition is combined with a new breeding method in a biological fermentation bed, which inhibits the growth and reproduction of other pathogenic microorganisms, effectively promotes the growth and reproduction of biological fermentation strains, and improves the degradation activity of the biological fermentation bed, and the concentration of ammonia in the fermen- tation bed and the environment is basically zero, so that the health of a breeding environment during chicken breeding can be ensured, thereby ensuring the health of bred chicken flocks, and reducing the mortality and elimination rate. In addition, the sur- vival rate of the whole flock is more than 96%, and the laying rate of local-breeding laying chickens is higher than 75% in peak time.

According to the chicken breeding mode of the present inven- tion, the biological fermentation bed is used in combination with a compound traditional Chinese medicine formula preparation, which achieves healthy, safe, efficient and ecological breeding of chickens, reduces the use of antibiotics, avoids residues of anti- biotic components in meat and egg products, ensures the quality safety of chicken products, and improves the flavor and functional nutrient components of the products. In laid eggs, cholesterol de- creases, beneficial unsaturated fatty acids increase, and flavor amino acids increase.

The Lactococcus lactis LAWH202106 was collected with collec- tion number of CCTCC NO: M 2021959 in China Center for Type Cul- ture Collection (CCTCC) at Wuhan University in Wuhan, China, on

July 30 2021.

The Enterococcus faecalis EFWH202105 was collected with col- lection number of CCTCC NO: M 2021958 in China Center for Type

Culture Collection (CCTCC) at Wuhan University in Wuhan, China, on

July 30 2021.

The Bacillus licheniformis BLHB-20200701 was collected with collection number of CCTCC NO: M 2020428 in China Center for Type

Culture Collection (CCTCC) at Wuhan University in Wuhan, China, on

August 17 2020.

DETAILED DESCRIPTION OF THE EMBODIMENTS

Embodiment 1

A traditional Chinese medicine micropowder composition, in- cluding the following raw materials: 10 kg of Fructus Arctii, 10 kg of Fructus Cnidii, 5 kg of Dichroa febrifuga Lour., 15 kg of

Astragalus membranaceus, 15 kg of wormwood, 10 kg of Sweet Worm- wood Herb, and 5 kg of Codonopsis pilosula.

A method for preparing the traditional Chinese medicine mi- cropowder composition includes: mixing the medicinal materials at a ratio, then pulverizing in a pulverizer, and sieving through a

No. 1 sieve.

Embodiment 2

The embodiment differs from Embodiment 1 in that: a tradi- tional Chinese medicine micropowder composition includes the fol- lowing raw materials: 9 kg of Fructus Arctii, 11 kg of Fructus

Cnidii, 6 kg of Dichroa febrifuga Lour., 14 kg of Astragalus mem- branaceus, 15 kg of wormwood, 10 kg of Sweet Wormwood Herb, and 5 kg of Codonopsis pilosula.

Embodiment 3

The embodiment differs from Embodiment 1 in that: a tradi- tional Chinese medicine micropowder composition includes the fol- lowing raw materials: 1lkg of Fructus Arctii, 11 kg of Fructus

Cnidii, 4kg of Dichroa febrifuga Lour., 15kg of Astragalus membra- naceus, l4kg of wormwood, 9kg of Sweet Wormwood Herb, and 4kg of

Codonopsis pilosula.

Comparative Example 1

The comparative example differs from Embodiment 1 in that: a traditional Chinese medicine micropowder composition includes the following raw materials: 10 kg of Fructus Cnidii, 5 kg of Dichroa febrifuga Lour., 7 kg of Astragalus membranaceus, 7 kg of worm- wood, 9 kg of Sweet Wormwood Herb, and 6 kg of Codonopsis pilosu- la.

Comparative Example 2

The comparative example differs from Embodiment 1 in that: a traditional Chinese medicine micropowder composition includes the following raw materials: 11 kg of Fructus Arctii, 4 kg of Dichroa 5 febrifuga Lour., 14 kg of wormwood, 9 kg of Sweet Wormwood Herb, and 4 kg of Codonopsis pilosula.

Embodiment 4

A fermentation bed for chicken breeding, characterized in that a padding thereof is obtained by mixing 80% of rice hulls and 20% of sawdust, 0.2% of the traditional Chinese medicine micropow- der composition prepared in Embodiment 1 is added to the padding, and after mixing well, the resulting mixture is laid in chicken houses with a laying thickness of 20 cm.

Embodiment 5

The embodiment differs from Embodiment 4 in that: 0.4% of the traditional Chinese medicine micropowder composition prepared in

Embodiment 1 is added to a padding.

Embodiment 6

The embodiment differs from Embodiment 4 in that: a fermenta- tion bed for chicken breeding has a laying thickness of 22 cm.

Comparative Example 3

The comparative example differs from Embodiment 4 in that: 0.2% of the traditional Chinese medicine micropowder composition prepared in Comparative Example 1 is added to a padding.

Comparative Example 4

The comparative example differs from Embodiment 4 in that: no traditional Chinese medicine micropowder composition is added to a padding.

Embodiment 7

A fermentation bed for chicken breeding, characterized in that a mixed microbial fermentation broth is evenly sprayed into the padding of Embodiment 4 through a sprayer, with 1.5 L of mixed microbial fermentation broth per square meter of padding.

Lactococcus lactis LAWH202106, Enterococcus faecalis

EFWH202105 and Bacillus licheniformis BLHB-20200701 are mixed at a ratio of 1:1:1 to obtain a mixed microbial strain. To 15 L of wa- ter (added with 1 kg of brown sugar in advance), 10 g of the mixed strain is added, and then sealed for fermentation at 25°C for 3 days to obtain a mixed microbial strain fermentation broth with viable count of the Lactococcus lactis, the Enterococcus faecalis and the Bacillus licheniformis of 5.2x10% cfu/ml, 5.5x10° cfu/ml and 5.5x10% cfu/ml respectively.

Embodiment 8

The embodiment differs from Embodiment 7 in that: Lactococcus lactis LAWH202106, Enterococcus faecalis EFWH202105 and Bacillus licheniformis BLHB-20200701 are mixed at a ratio of 1:2:2 to ob- tain a mixed microbial strain. To 15 L of water (added with 1kg of brown sugar in advance), 10 g of the mixed strain is added, and then sealed for fermentation at 24°C for 3 days to obtain a mixed microbial strain fermentation broth with viable count of the Lac- tococcus lactis, the Enterococcus faecalis and the Bacillus 1i- cheniformis of 5.5x10% cfu/ml, 5.4x10° cfu/ml and 5.6x10° cfu/ml respectively.

Embodiment 9

The embodiment differs from Embodiment 7 in that: Lactococcus lactis LAWH202106 and Enterococcus faecalis EFWH202105 are mixed at a ratio of 1:1 to obtain a mixed microbial strain. To 15 L of water (added with lkg of brown sugar in advance), 10 g of the mixed strain is added, and then sealed for fermentation at 25°C for 3 days to obtain a mixed microbial strain fermentation broth with viable count of the Lactococcus lactis and the Enterococcus fae- calis of 5.5x10° cfu/ml and 5.4x10° cfu/ml respectively.

Embodiment 10

A breeding mode for local-breeding laying chickens, charac- terized in that local-breeding laying chickens are bred in a fer- mentation bed for chicken breeding, and traditional Chinese medi- cine bags are hung in chicken houses. Raw materials of the tradi- tional Chinese medicine bags include 10 parts of wormwood, 10 parts of Sweet Wormwood Herb and 10 parts of Acorus calamus, with the weight of each traditional Chinese medicine bag of 100 g, and one bag for every approximately 100 m°. The bags are evenly dis- tributed in the chicken houses, 1.2 m above the ground.

During breeding of local-breeding laying chickens, tradition-

al Chinese medicine extracts are added to drinking water: 1.5% of an Astragalus extract is added on Day 0-7; 0.8% of a traditional

Chinese medicine extract for preventing and treating chicken diar- rhea is added on Day 8-14; 0.8% of a traditional Chinese medicine extract for preventing and treating avian colibacillosios is added on Day 15-21 and Day 91-98; 0.8% of a traditional Chinese medicine extract for preventing and treating coccidiosis in chicken is add- ed on Day 22-27, Day 42-49 and Day 63-70; and 1.5% of a mixed pow- der of Astragalus membranaceus, motherwort and Cortex Phellodendri is added on Day 127-133.

Embodiment 11

The embodiment differs from Embodiment 10 in that: during breeding of local-breeding laying chickens, traditional Chinese medicine extracts or compound powder is not added to drinking wa- ter and feed, and only corresponding antibiotic agents are used to treat local-breeding laying chickens with different diseases.

Embodiment 12

In March 2019, an experiment was carried out in a farm of lo- cal-breeding laying chickens (a local chicken breed) in Huangpi

District, Wuhan, a fermentation bed + underwood stocking mode was used, 6000 30-day-old chicks were raised, randomly divided into © groups, and raised in 6 chicken houses. The breeding mode in Em- bodiment 10 was used for experimental groups 1-5, and the breeding mode in Embodiment 11 was used for an experimental group 6.

The fermentation bed for breeding local-breeding laying chickens in Embodiment 4 was used for the experimental group 1;

The fermentation bed for breeding local-breeding laying chickens in Embodiment 3 was used for the experimental group 2;

The fermentation bed for breeding local-breeding laying chickens in Embodiment 4 was used for the experimental group 3;

The fermentation bed for breeding local-breeding laying chickens in Embodiment 7 was used for the experimental group 4;

The fermentation bed for breeding local-breeding laying chickens in Embodiment 9 was used for the experimental group 5;

The fermentation bed for breeding local-breeding laying chickens in Comparative Example 4 was used for the experimental group 6.

After the local-breeding laying chickens in each group were raised to 270-day-old, average laying rate, average egg weight, and mortality and elimination rate of the local-breeding laying chickens in each group were counted. The results are shown in Ta- ble 1.

Table 1 Comparison of average laying rate, average egg weight, and mortality and elimination rate of local-breeding lay- ing chickens in each group “Group Experimental Average lay- Average Mortality chickens ing rate (%) egg weight and elimi- (Nr. ) (9) nation rate (2)

Experimental 1000 68.8 37.6 5.9 group 1

Experimental 1000 €7.9 36.8 6.2 group 2

Experimental 1000 63.1 35.9 7.6 group 3

Experimental 1000 72.4 38.5 4.2 group 4

Experimental 1000 71.3 37.3 3.9 group 5

Experimental 1000 53.2 34.5 16.5 group 6

As can be seen from Table 1, the chicken breeding mode of the present invention can effectively improve laying rate and egg weight of the local-breeding laying chickens, and reduce mortality and elimination rate of the local-breeding laying chickens.

Twenty eggs were randomly selected from each group to test cholesterol content, total content of 16 amino acids, oleic acid content, and linoleic acid content of the eggs. The results are shown in Table 2.

Table 2 Comparison of egg quality among groups

Group Cholesterol Total content Oleic acid Linoleic (mg/1009g) of 16 amino (g/1009) acid acids (g/100 (g/1009) g)

Experimental 168 10.2 0.1917 Not de-

group 1 tected

Experimental 167 10.3 0.2361 Not de- group 2 tected

Experimental 168 10.2 0.0597 Not de- group 3 tected

Experimental 156 10.6 0.4180 0.0758 group 4

Experimental 157 10.6 0.4080 0.0735 group 5

Experimental 182 10.0 0.0370 Not de- group 6 tected

As can be seen from Table 2, addition of a compound Chinese _ herbal medicine during breeding has the effect of increasing the total content of 16 amino acids.

The concentration of ammonia in the fermentation bed at Day 20, Day 40, Day 60, Day 80, Day 100, Day 120, Day 140, Day 160,

Day 180, Day 200, Day 220, Day 240 and Day 260 during breeding of the local-breeding laying chickens in each group was tested re- spectively, and average values of results from 13 tests were com- pared. The results are shown in Table 3.

Table 3 Test of concentration of ammonia in fermentation bed (PPM) "Group Experi- Ezperi- Experi- Experi- Experi- Ezperi- - mental mental mental mental mental mental group 1 group 2 group 3 group 4 group 5 group 6

Concen- 0,67 0.64 1.531 0.28 0.26 1.532 tration of ammo- nia

As can be seen from Table 3, the concentration of ammonia in the experimental group 5 was reduced by 61.19% compared with that in the experimental group 1, indicating that the concentration of ammonia can be significantly reduced by adding the mixed microbial fermentation broth to the fermentation bed for breeding. The con- centration of ammonia in the experimental group 5 was reduced by 83.02% compared with that in the experimental group 3, indicating that the present invention can significantly reduce the concentra-

tion of ammonia.

Embodiment 13

Local-breeding broiler chickens are bred in a fermentation bed for chicken breeding, and traditional Chinese medicine bags are hung in chicken houses. Raw materials of the traditional Chi- nese medicine bags include 10 parts of wormwood, 10 parts of Sweet

Wormwood Herb and 10 parts of Acorus calamus, with the weight of each traditional Chinese medicine bag of 100 g, and one bag for every 100 mè. The bags are evenly distributed in the chicken hous- es, 1.0 m above the ground.

During breeding of the local-breeding broiler chickens, tra- ditional Chinese medicine extracts are added to drinking water: 1.0% of an Astragalus extract is added on Day 0-7; 0.6% of a tra- ditional Chinese medicine preparation extract for preventing and treating chicken diarrhea is added on Day 8-14; 0.6% of a tradi- tional Chinese medicine preparation extract for preventing and treating avian colibacillosios is added on Day 15-21; 0.8% of a traditional Chinese medicine preparation extract for preventing and treating coccidiosis in chicken is added on Day 22-27 and Day 42-49; and 0.5% of an Astragalus extract is added on Day 63-70.

Embodiment 14

The embodiment differs from Embodiment 13 in that: during breeding of local-breeding broiler chickens, traditional Chinese medicine extracts are not added to drinking water, and only corre- sponding antibiotic agents are used to treat local-breeding broil- er chickens with different diseases.

Embodiment 15

In March 2021, an experiment was carried out in a farm of lo- cal-breeding broiler chickens (a local chicken breed) in Huangpi

District, Wuhan, a fermentation bed + underwood stocking mode was used, 3000 0-day-old chicks were raised, randomly divided into 6 groups, and raised in 6 chicken houses. The breeding mode in Em- bodiment 13 was used for experimental groups 7-11, and the breed- ing mode in Embodiment 14 was used for an experimental group 12.

The fermentation bed for chicken breeding in Embodiment 4 was used for the experimental group 7;

The fermentation bed for chicken breeding in Comparative Ex-

ample 3 was used for the experimental group 8;

The fermentation bed for chicken breeding in Comparative Ex- ample 4 was used for the experimental group 9;

The fermentation bed for chicken breeding in Embodiment 7 was used for the experimental group 10;

The fermentation bed for chicken breeding in Embodiment 9 was used for the experimental group 11;

The fermentation bed for chicken breeding in Comparative Ex- ample 4 was used for the experimental group 12.

After the chickens in each group were raised to 75-day-old, average weight, and overall mortality and elimination rate of 70- day-old chickens in each group were counted. The results are shown in Table 4.

Table 4 Comparison of average weight, and overall mortality and elimination rate of 70-day-old chickens in each group “Group Experimental Average weight of Mortality and chickens (Nr.) 70-day-old chickens elimination (kg) rate (3%)

Ezperimental 500 1.712 2.0 group 7

Experimental 500 1.637 2.8 group 8

Experimental 500 1.614 3.8 group 9

Experimental 500 1.867 1.4 group 10

Experimental 500 1.853 1.0 group 11

Experimental 500 1.517 5.2 group 12 © As can be seen from Table 4, the average weight of the 70- day-old broiler chickens was the highest in the experimental group 10, and the lowest mortality and elimination rate was observed in the experimental group 11, which decreased by 4.2% compared with that in the experimental group 12, indicating that the chicken breeding mode in the present invention can effectively improve the average weight of broiler chickens and reduce the mortality and elimination rate.

Claims

CONCLUSIONS

A micropowder composition for traditional Chinese medicine, comprising the following raw materials by weight: 3 - 12 parts Fructus Arctii, 2 - 12 parts Fructus Cnidii, 1 - 6 parts Dichroa febrifuga Lour., 5 - 16 parts Astragalus membranaceus, 5 - 16 parts wormwood, 5 - 12 parts sweet wormwood herb and 1 - 6 parts Codonopsis pilosula.

The application of the micropowder composition for traditional Chinese medicine according to claim 1 to a culture bed for breeding, characterized in that the fermentation bed is a chicken breeding fermentation bed.

A fermentation bed for raising chickens comprising a filling and 0.05-0.5% by weight of the micropowder composition for traditional Chinese medicine according to claim 1.

The fermentation bed for raising chickens according to claim 3, further comprising a fermentation broth of a mixed microbial strain.

A fermentation bed for raising chickens according to claim 4, characterized in that the mixed microbial strain is prepared from Lactococcus lactis LAWH202106, Enterococcus faecalis EFWH202105 and Bacillus licheniformis BLHB-20200701 in a ratio of 1-2:1-2:1 -2.

A fermentation bed for raising chickens according to claim 5, characterized in that in the fermentation broth of the mixed microbial strain, the viable count of the Lactococcus lactis LAWH202106, the Enterococcus faecalis EFWH202105 and the Bacillus licheniformis BLHB-20200701 is 4 - 6 x 10° CFU/ml, 4 - 6 x 10° CFU/ml and 4 - 6 x 10° CFU/ml, respectively.

A fermentation bed for raising chickens according to claim 3,

characterized in that the dosage of the fermentation broth is 0.5 - 1.6 L/m2.

A chicken breeding mode, comprising: raising chickens in the fermentation bed for raising chickens according to any one of claims 3 to 7, hanging traditional Chinese medicine bags in chicken houses, and adding traditional Chi - nese medicine preparations or traditional Chinese medicine extracts to feed or drinking water during the breeding of the chickens.