WO2020204395A3 - 독소의 제조방법 - Google Patents

독소의 제조방법 Download PDF

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Publication number
WO2020204395A3
WO2020204395A3 PCT/KR2020/003458 KR2020003458W WO2020204395A3 WO 2020204395 A3 WO2020204395 A3 WO 2020204395A3 KR 2020003458 W KR2020003458 W KR 2020003458W WO 2020204395 A3 WO2020204395 A3 WO 2020204395A3
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WO
WIPO (PCT)
Prior art keywords
medium
botulinum toxin
production
present
preparing
Prior art date
Application number
PCT/KR2020/003458
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English (en)
French (fr)
Other versions
WO2020204395A2 (ko
Inventor
김재영
남정선
김승호
김민중
이원일
안향미
Original Assignee
(주)제테마
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by (주)제테마 filed Critical (주)제테마
Priority to CN202080034761.2A priority Critical patent/CN113811616A/zh
Priority to BR112021019233A priority patent/BR112021019233A2/pt
Priority to US17/599,696 priority patent/US20220195412A1/en
Priority to EP20784897.9A priority patent/EP3950951A4/en
Priority to MX2021011744A priority patent/MX2021011744A/es
Publication of WO2020204395A2 publication Critical patent/WO2020204395A2/ko
Publication of WO2020204395A3 publication Critical patent/WO2020204395A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24069—Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/145—Clostridium

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 포테이토 펩톤(potato peptone), 효모 추출물(yeast extract) 및 글루코스(glucose)를 포함하는 클로스트리디움 보툴리눔 배양을 위한 배지용 배지 조성물 및 이를 이용한 보툴리눔 독소의 제조방법에 관한 것으로, 본 발명에 따르면 상기 배지는 동물 유래 성분 및 주요 알레르기 유발물질이 배제된 배지로써, 단시간 내에 보툴리눔 독소 생산 균주가 최대 성장량에 도달할 수 있도록 유도하여, 보툴리눔 독소 생산 공정에서의 생산 시간을 단축시키고 생산 단가를 절감할 수 있는 효과가 있다.
PCT/KR2020/003458 2019-03-29 2020-03-12 독소의 제조방법 WO2020204395A2 (ko)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CN202080034761.2A CN113811616A (zh) 2019-03-29 2020-03-12 制备毒素的方法
BR112021019233A BR112021019233A2 (pt) 2019-03-29 2020-03-12 Método para preparar toxina botulínica
US17/599,696 US20220195412A1 (en) 2019-03-29 2020-03-12 Method of preparing toxin
EP20784897.9A EP3950951A4 (en) 2019-03-29 2020-03-12 TOXIN PREPARATION METHOD
MX2021011744A MX2021011744A (es) 2019-03-29 2020-03-12 Metodo de preparacion de toxina.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2019-0036921 2019-03-29
KR1020190036921A KR102209159B1 (ko) 2019-03-29 2019-03-29 독소의 제조방법

Publications (2)

Publication Number Publication Date
WO2020204395A2 WO2020204395A2 (ko) 2020-10-08
WO2020204395A3 true WO2020204395A3 (ko) 2020-11-19

Family

ID=72666801

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2020/003458 WO2020204395A2 (ko) 2019-03-29 2020-03-12 독소의 제조방법

Country Status (8)

Country Link
US (1) US20220195412A1 (ko)
EP (1) EP3950951A4 (ko)
KR (1) KR102209159B1 (ko)
CN (1) CN113811616A (ko)
BR (1) BR112021019233A2 (ko)
MX (1) MX2021011744A (ko)
TW (1) TWI748394B (ko)
WO (1) WO2020204395A2 (ko)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20230057586A1 (en) * 2019-08-16 2023-02-23 Janssen Biotech, Inc. Optimized culture media for clostridia bacteria
KR20240041474A (ko) * 2022-09-23 2024-04-01 (주)제테마 보툴리눔 독소 생산성을 증가시키는 방법

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006042542A2 (en) * 2004-10-19 2006-04-27 Statens Serum Institut Production of tetanus, diphtheria, and pertussis toxins and toxoids using fermentation media containing no components of animal or soy origin
KR20090120222A (ko) * 2008-05-19 2009-11-24 (주)메디톡스 식물 유래 성분 함유 배지 및 가요성 폐쇄 용기를 이용하여클로스트리디움 보툴리눔 독소를 생산하는 방법
KR20090129362A (ko) * 2008-06-11 2009-12-16 에프. 호프만-라 로슈 아게 포유류 공급원 성분이 없는 ｃ. 히스톨리티쿰용 성장 배지
KR20160127997A (ko) * 2015-04-28 2016-11-07 주식회사 대웅 보툴리눔 독소의 제조를 위한 배지 조성물

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7148041B2 (en) 2003-09-25 2006-12-12 Allergan, Inc. Animal product free media and processes for obtaining a botulinum toxin
US8129139B2 (en) * 2009-07-13 2012-03-06 Allergan, Inc. Process for obtaining botulinum neurotoxin
KR101729251B1 (ko) * 2015-04-28 2017-04-21 주식회사 대웅 보툴리눔 독소의 제조를 위한 배지 조성물
CA3060669A1 (en) * 2017-04-28 2018-11-01 Bonti, Inc. Botulinum neurotoxins production methods
KR102197224B1 (ko) * 2019-03-29 2020-12-31 (주)제테마 배지 조성물
TW202136518A (zh) * 2019-12-20 2021-10-01 瑞士商葛德瑪控股公司 生產肉毒桿菌毒素的方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006042542A2 (en) * 2004-10-19 2006-04-27 Statens Serum Institut Production of tetanus, diphtheria, and pertussis toxins and toxoids using fermentation media containing no components of animal or soy origin
KR20090120222A (ko) * 2008-05-19 2009-11-24 (주)메디톡스 식물 유래 성분 함유 배지 및 가요성 폐쇄 용기를 이용하여클로스트리디움 보툴리눔 독소를 생산하는 방법
KR20090129362A (ko) * 2008-06-11 2009-12-16 에프. 호프만-라 로슈 아게 포유류 공급원 성분이 없는 ｃ. 히스톨리티쿰용 성장 배지
KR20160127997A (ko) * 2015-04-28 2016-11-07 주식회사 대웅 보툴리눔 독소의 제조를 위한 배지 조성물

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SOLOMON, H. M. ET AL.: "Growth and toxin production by Clostridium botulinum in sliced raw potatoes under vacuum with and without sulfite", JOURNAL OF FOOD PROTECTION, vol. 57, no. 10, 1994, pages 878 - 881, XP055753393 *

Also Published As

Publication number Publication date
EP3950951A2 (en) 2022-02-09
US20220195412A1 (en) 2022-06-23
TW202102681A (zh) 2021-01-16
BR112021019233A2 (pt) 2021-11-30
TWI748394B (zh) 2021-12-01
EP3950951A4 (en) 2023-03-29
KR102209159B1 (ko) 2021-01-29
KR20200114721A (ko) 2020-10-07
CN113811616A (zh) 2021-12-17
WO2020204395A2 (ko) 2020-10-08
MX2021011744A (es) 2022-01-06

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