WO2020122149A1 - 病理組織検体梱包素材及び組織切片のコンタミネーション防止用袋 - Google Patents

病理組織検体梱包素材及び組織切片のコンタミネーション防止用袋 Download PDF

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Publication number
WO2020122149A1
WO2020122149A1 PCT/JP2019/048574 JP2019048574W WO2020122149A1 WO 2020122149 A1 WO2020122149 A1 WO 2020122149A1 JP 2019048574 W JP2019048574 W JP 2019048574W WO 2020122149 A1 WO2020122149 A1 WO 2020122149A1
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WIPO (PCT)
Prior art keywords
compartment
tissue section
bag
bag according
tissue
Prior art date
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Ceased
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PCT/JP2019/048574
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English (en)
French (fr)
Japanese (ja)
Inventor
啓一 岩屋
正義 相馬
圭司 上田
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Sasaki Foundation Sasaki Institute
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Sasaki Foundation Sasaki Institute
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Priority to JP2020559298A priority Critical patent/JP7449237B2/ja
Priority to CN201980081886.8A priority patent/CN113167692A/zh
Publication of WO2020122149A1 publication Critical patent/WO2020122149A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q

Definitions

  • the present invention relates to a pathological tissue sample packing material that prevents cell permeation, and a tissue section contamination prevention bag ( ⁇ -filter sheet pocket ( ⁇ SP)) in the process of making a formalin-fixed paraffin-embedded block.
  • ⁇ -filter sheet pocket ⁇ -filter sheet pocket
  • Non-patent Document 1 a dialysis membrane applicable to emergency use is known as a medical product using the technology of thin fibers.
  • an antibacterial filter film has been developed by combining polyvinylidene fluoride (PVDF) film, polyestersulfone (PES) film, and cellulse acetate (CA) with an antibacterial substance (Non-patent document 2).
  • Non-Patent Document 3 Materials for preventing cell permeation and allowing various solutions to pass through are necessary for the preparation of pathological tissue specimens, but hitherto there has been no technique or information for overcoming this problem.
  • the conventional nylon mesh bag was able to prevent leakage at the tissue level, but could not prevent leakage at the cell level (contamination). From the above, there has been a demand for development of a material that prevents cell permeation and allows various solutions to pass through.
  • the present inventor has conducted extensive studies to solve the above problems, and as a result, has completed the present invention by using ⁇ SP having two predetermined compartments and having a predetermined average mesh size. It was
  • the present invention is as follows.
  • a material for packaging a tissue section which satisfies the conditions of at least three of the following six evaluation items.
  • Mean diameter of mesh 5 ⁇ m or less
  • Thickness 100 ⁇ m or less
  • Chemical resistance No significant change in dissolution, deterioration or discoloration by visual observation
  • Water permeability Water permeability coefficient is 10 ⁇ 3 or more
  • Water retention rate Water retention rate is less than 200%
  • Nucleated cells Passage 2 to less than 2 cells are observed in 10 fields of view in a high-power field of 200 to 600 times
  • a bag for preventing contamination of a tissue section which has a first compartment that is at least partially open, and an internal space that is smaller than the internal space of the first compartment and that is at least partially open It has a 2nd division room for arranging a section, and a connecting part which connects the 1st division room and the 2nd division room, and an opening of the 1st division room and an opening of the 2nd division room.
  • the contamination-preventing bag is opposed to the section, and the second compartment is accommodated in the first compartment when the second compartment is folded back toward the first compartment.
  • the bag according to (5) which is made of a material having a mesh average diameter that does not allow cells to pass through.
  • the bag according to (5) which is made of the material according to any one of (1) to (4).
  • the bag according to (5) wherein each of the first compartment and the second compartment is rectangular.
  • the bag according to (5), wherein the first compartment, the second compartment, and the connecting portion are formed by one sheet.
  • a heat seal part is provided in a part of the first compartment and/or the second compartment.
  • a method for immersing a tissue section which comprises immersing the tissue section in a fixative or a solvent using the bag according to any one of (5) to (11).
  • a method for preventing contamination of a tissue section which comprises immersing the tissue section in a fixative or a solvent using the bag according to any one of (5) to (11).
  • the present invention provides a material for packing a pathological tissue specimen that prevents cells from permeating and allows various solvents to pass through. By using the material of the present invention, it became possible to prevent contamination at the cell level. Further, while the conventional nylon mesh bag prevents leakage at the tissue level, the present invention makes it possible to prevent contamination at the cell level including cell fragments. By using the bag of the present invention, it is possible to ensure the improvement of the quality of the tissue section, for example, the material used for genomic medicine.
  • FIG. 3 is a plan view of a developed ⁇ SP of the present invention. It is a figure which shows having accommodated the tissue section in the 2nd compartment of ⁇ SP of the present invention. It is a figure which shows a mode that a tissue section is accommodated in the 1st compartment of ⁇ SP of the present invention. It is a schematic diagram of ⁇ SP of the present invention provided with a heat seal part and a weight accommodating part.
  • FIG. 7 is a plan view of a developed ⁇ SP of the present invention provided with a heat seal portion and a weight accommodating portion.
  • FIG. 7 is a schematic view showing that the tissue section is housed in the second compartment of the ⁇ SP of the present invention provided with the heat seal part and the weight housing part, and the weight is housed in the weight housing part. It is a figure which shows how to make a test filter assembly. It is a figure which shows the actual thing of ⁇ SP of this invention. It is a figure which shows the cell image counted in the passage test. It is a figure which shows the result of having investigated the presence or absence of the contamination of cells using (mu) SP of this invention. It is a figure which shows the tissue section accommodated in the 2nd division chamber and the weight was accommodated in the weight accommodation part in the actual ⁇ SP provided with the heat seal part and the weight accommodation part.
  • the present invention provides a material for packing pathological tissue, which is mainly used for producing a high-quality pathological tissue specimen that supports genomic medicine.
  • a material that does not pass through cells and easily passes through various solutions was screened, and a material that can be used in the fields of medicine and biotechnology was found.
  • the conventional nylon mesh bag can prevent leakage at the tissue level, it cannot prevent leakage at the cell level (contamination).
  • the present invention makes it possible to prevent contamination at the cell level including cell fragments.
  • the bag made of the material of the present invention it is possible to ensure the improvement of the quality of the tissue section, for example, the material used for genomic medicine.
  • the material is easily processed by heat welding (heat sealing) and can be processed into a shape that encloses a pathological tissue or a cassette without using an organic solvent.
  • the screening method of the present invention is used to verify a material used as a contamination prevention measure in a pathology laboratory.
  • the cells used in the present invention are cells fixed with formalin or the like, but are not limited thereto, and living cells or tissues can also be used.
  • tissue section that can be a target of a pathological tissue specimen.
  • tissue section may be a collected tissue section itself, a pathological tissue sample mounted on a slide glass, a cassette in which a tissue section is placed in a plate having wells, or a body cavity. It may be a cell block collected from a liquid and is not particularly limited.
  • the material of the present invention can be used in various application ranges depending on the application.
  • the material of the present invention can be used during paraffin infiltration treatment, degreasing/refixing work, or storage, and also when cell blocks or fragile tumor tissues are prevented from spreading to the outside. Can be used.
  • the design and size of the bag in which the material is used are not particularly limited and may be various.
  • Examples of the material used in the present invention include nylon, polyester, tetrafluoroethylene resin (PTEF), non-woven fabric (fine fiber sheet), and a composite synthetic sheet of a combination thereof.
  • the material of the present invention has the following properties by various tests.
  • the material of the present invention has the following properties.
  • Mean diameter of mesh The mesh structure of the material of the present invention is not limited as long as cells do not pass through.
  • the size of the mesh gap refer to the catalog value, but simply check with a polarizing microscope.
  • the average mesh size is 5 ⁇ m or less, and is 1 to 5 ⁇ m, preferably 1 ⁇ m.
  • Thickness 100 ⁇ m or less
  • the thickness of the material of the present invention is not limited as long as it does not interfere with the working process of the pathological tissue block.
  • the thickness refer to the catalog value, but it is preferably 100 ⁇ m or less so as not to prevent bending and folding in bag processing and heat welding.
  • Chemical resistance can be measured by a chemical resistance test (JIS A 5209:2014). Specifically, it is as follows. Place 100% ethanol and 100% xylene in a container with a lid. After cutting the sample in half, one sample is entirely immersed in the test solution and the other sample serves as a comparison control. Cover with a lid and hold at room temperature for about 8 hours. After that, the sample is washed with water and dried, and then visually confirmed. The visual confirmation is based on significant changes such as dissolution by chemicals, brittle fracture, and deterioration (color, gloss, etc.). As a result of the confirmation, if there is no visible change in at least dissolution, deterioration or discoloration, it is judged that the packaging material can withstand the organic solvent in the block making process.
  • the water permeability can be measured by a modified method of the water level permeability test (JIS A 1218). The range is as follows. Then, if the water permeability is at least 10 ⁇ 3 , for example, 10 ⁇ 3 or more, it is determined that various solvents can be promptly exchanged in the block making process.
  • Water retention can be measured by a general nonwoven fabric test method (JIS 1913:2019). The contents are as follows. Three 100 mm x 100 mm test pieces are taken from the sample, and the mass is measured up to 1 mg. Pour water into an appropriately sized container and soak the specimen for at least 15 minutes. Next, remove the test piece from the water with tweezers, drop the water on it for 1 minute or more, and drop it, and then measure its mass up to 1 mg.
  • the passability of nucleated cells can be measured by the mutatis mutandis of the test method (JIS K3835:1990) of the bacteria capturing performance of microfiltration membrane elements and modules.
  • Thick and thin cylinders are prepared by cutting off the tips of 1000 ⁇ l microchip and 200 ⁇ l microchip, and a test filter assembly is prepared by sandwiching a sheet between the thick cylinder and the thin cylinder. This is packed with Parafilm and attached to a 1.5 ml tube for collecting the filtrate.
  • a cell suspension is prepared by placing cells in a 10% formalin solution. Fill the test filter assembly with 600 ⁇ l of 10% formalin cell suspension. Collect the filtrate by gravity filtration into a 1.5 ml tube. After adding 100% ethanol to the slide glass, immediately add 15 ⁇ l of the filtrate. After natural drying, the color is developed by Papanicolaou dyeing. The number of red blood cells and nucleated cells that have passed through the eyes of the sheet is counted in 10 fields of view at high magnification, for example, 200 to 600 times, preferably 400 times. Passage of nucleated cells: Observe 10 places in a high field of view of a microscope with a magnification of 400 times. If the total number of nucleated cells is 2 or less, it is judged as an appropriate range as a packaging material. To do.
  • sheets satisfying the conditions described in at least three items can be adopted in the present invention, but sheets corresponding to 4 to 5 items are preferable, and sheets corresponding to all items are more preferable. preferable.
  • the material of the present invention is heated at 100°C to 400°C (when the material is heated from 100°C to 400°C), the materials are melted and the materials are bonded to each other.
  • the sheet of the above material is not particularly limited in shape and the like as long as it can wrap the tissue section, and may be rectangular or circular. Further, not only a single layer but also a double layer or a triple layer can be stacked. In the case of overlapping two or more layers, it is possible to use a sheet whose size is changed stepwise. By overlapping the cells in double or triple layers, it is possible to more effectively prevent the contamination of cells with the tissue section.
  • the present invention provides a tissue section contamination prevention bag.
  • the contamination prevention bag of the present invention is referred to as a " ⁇ -filter sheet pocket" and is referred to as " ⁇ SP" in the present specification.
  • the ⁇ SP of the present invention includes a first compartment that is at least partially open, a second compartment that has an internal space that is smaller than the internal space of the first compartment, and that is at least partially open, and the first compartment. And a connecting portion that connects the chamber and the second partitioned chamber.
  • the second compartment is a compartment for arranging a tissue section in its internal space. The opening of the first compartment and the opening of the second compartment are opposed to each other, and when the second compartment is folded back toward the first compartment, the second compartment is It is housed in the first compartment.
  • FIG. 1 A ⁇ SP of one embodiment of the present invention is shown in FIG.
  • the ⁇ SP1 of the present invention includes a first compartment 10, a second compartment 20, and a connecting portion 30 that connects the first compartment 10 and the second compartment 20.
  • each of the first compartment 10 and the second compartment 20 has a rectangular shape, and a part thereof is open.
  • the shapes of the first compartment 10 and the second compartment 20 are not particularly limited, and may be rectangular, circular, or elliptical.
  • one side of the first compartment 10 forms the opening 50a and one side of the second compartment 20 forms the opening 50b.
  • the opening 50a and the opening 50b face each other and face each other.
  • the connection part 30 is a region that connects between the opening 50a of the first compartment 10 and the opening 50b of the second compartment 20 and has a predetermined length for wrapping the second compartment containing the tissue section.
  • the inner space (space) of the second compartment 20 is smaller than the inner space of the first compartment 10, and when the second compartment 20 is folded as described later, the folded compartment 20 is folded. Are accommodated in the internal space of the first compartment 10. Further, in FIG. 1, in the ⁇ SP1 of the present invention, the protruding portion 40 can be formed at the opening 50a portion of the first partitioned chamber 10.
  • FIG. 2 is a plan view of the expanded ⁇ SP1 shown in FIG.
  • the original material ( ⁇ -filter sheet) made of one sheet in the shape of the plan view is mountain-folded and valley-folded at predetermined positions to obtain ⁇ SP1 shown in FIG. It can be made.
  • the sizes or lengths of the first compartment 10, the second compartment 20, and the connecting portion 30 are not particularly limited as long as the paraffin-embedded tissue section preparation cassette can be accommodated and the mouth of the bag can be enclosed.
  • the second compartment 20 has a long side when developed of preferably 150 mm to 160 mm and a short side of 70 mm to 95 mm (preferably 70 mm to 75 mm for standard type and 90 mm to 95 mm for king size). ..
  • the length of the first compartment 10 may be adjusted so that the second compartment 20 can be accommodated and the opening can be enclosed.
  • the first section has a long side (I 1 +I 2 +I 3 ) of 170 mm to 180 mm (the long side of a bag is 70 mm to 75 mm) when expanded, and the short side is 90 mm to 115 mm (preferably Is 90 mm to 95 mm for the standard type and 110 mm to 115 mm for the king size.
  • the long side of the connecting portion 30 when expanded is preferably 75 mm to 85 mm.
  • valley folding is performed at an arbitrary position x 1 -y 1 in a region forming the first partitioned chamber 10 and valley folding is performed at an arbitrary position x 2 -y 2 in a region forming the second partitioned chamber 20.
  • a bonding margin 60 having an appropriate width on the longitudinal side surface of the compartment when folded in a valley shape and bonding the portion, the openings 50a, 50b in the first compartment 10 and the second compartment 20 are formed.
  • the sides other than are closed to form a bag-shaped space.
  • the protruding region 40 can be formed in the first partitioned chamber 10 by mountain-folding at the position of x 3 ⁇ y 3 .
  • FIG. 3 is a view showing a mode in which the tissue section 70 is housed in the second compartment 20.
  • the "tissue section” referred to here may be the collected tissue section or the cell aggregate itself collected from the body cavity fluid, or the one embedded in OCT compound as a rapid pathological tissue specimen, in a plate equipped with wells, etc. It may be a cassette containing cell clumps or tissue sections and is not particularly limited. After inserting the tissue section 70 into the internal space of the second compartment 20 from the opening 50b of the second compartment 20, the tissue section 70 is valley-folded and folded several times to wrap the tissue section. The second compartment 20 enclosing the tissue section 70 is slid into the internal space of the first compartment 10 to be stored.
  • A shows a mode in which the second partitioned chamber 20 is slid into the internal space from the opening 50a of the first partitioned chamber 10
  • B is a protruding portion 40 provided in the first partitioned chamber 10 to cover the lid.
  • the mode when doing is shown.
  • the protruding portion 40 can function as a lid by storing the second partitioned chamber 20 in the inner space of the first partitioned chamber 10 and then folding the protruding portion back to the opposite side. Therefore, the opening 50a of the first compartment 10 can be firmly closed by folding it inward at the protruding portion 40 and further by using a stapler or the like.
  • FIGS. 5 is a diagram in which the weight mounting portion 80 and the heat seal portion 90 are provided in the second compartment
  • FIG. 6 is a development view of FIG.
  • FIG. 7 is a diagram in which the tissue section 70 is accommodated in the second compartment and the weight 81 is accommodated in the weight accommodating portion 80.
  • the weight mounting part can be formed into a bag by heat-welding the folded side edge part to the main body so that the weight can be housed inside.
  • the weight stainless steel, brass or the like is used, and the weight is 1 g to 100 g, preferably 4.5 g to 5.5 g.
  • the shape of the weight is not particularly limited as long as it does not protrude from the bag of the accommodating portion, but an elongated rectangular shape or an elongated cylindrical shape is used.
  • the heat seal part 90 can be provided in a part of the first compartment, a part of the second compartment, or both of them, and in the case of the first compartment, a corner of the protruding portion 40 and the second compartment. In the case of, the corner can be formed on the right side of the second compartment. 5 and 7, the boundary of the heat seal portion 90 is shown by a broken line (---) and the heat seal portion has a triangular shape, but the shape is not limited to the triangle and the shape is arbitrary.
  • the heat-sealing section 90 heat-bonds the thermoplastic resin sheets of the same quality to each other without using an adhesive agent. By providing the heat-sealing portion, it is possible to obtain the effect of obtaining a structure that can withstand immersion in a solvent such as xylene.
  • the materials described in the section “1. Material for packaging pathological tissue” can be used.
  • the material of ⁇ SP used in the present invention includes a composite synthetic sheet made of nylon, polyester, polyfluoroethylene, or a combination thereof.
  • the mesh structure of the ⁇ SP of the present invention is not limited as long as it does not pass cells, and the size of the mesh gap is 1 ⁇ m to 5 ⁇ m.
  • the sample can penetrate without causing contamination by other cells.
  • the tissue section contained in the ⁇ SP of the present invention may be immersed and left as it is, or may be appropriately stirred.
  • the exchange of the fixative or the solvent is optional and may be performed once or twice or more.
  • the test filter assembly was made as follows (FIG. 8). Prepare one 1000 ⁇ l microchip and two 200 ⁇ l chips, cut them with scissors, and cut the 1000 ⁇ l microchip 2.5 cm (a) and the remaining tip (a), 200 ⁇ l chip 2 cm (c), 200 ⁇ l chip 3 cm ( D) was prepared. The tip portion (a) of 1000 ⁇ l was used for adjusting the thickness of the sheet. The tube was firmly inserted in the order of d ⁇ w ⁇ a (FIG. 8 panel A), and the excess part of a was cut off (arrow in FIG. 8 panel B).
  • a sheet (3 cm x 3 cm) of a predetermined material (Table 1) was covered on the above-mentioned arrow portion (cut portion) and inserted into (a). A sheet was inserted between (a) and (a) or (c) was assembled to the outer diameter, and the sheet was fixed. The sheet protruding from the device was cut off. In order to prevent air trapping, (A) was aligned with the fixed position of the filtration sheet, and the excess portion was cut off (arrow in panel C). Parafilm was wound to prevent liquid leakage (FIG. 8 panel D) to prepare a test filter. The filter thus manufactured was used for a predetermined test.
  • the five candidate materials for packaging tissue sections have an average mesh size of 5 ⁇ m or less, drug resistance, and the number of nucleated cells passing through is 2 or less. It is desirable that the water retention rate is less than 200%, and the hydraulic conductivity is 1.0x10 -3 or more.
  • a first compartment having at least a part opened, a second compartment having an inner space smaller than the inner space of the first compartment and having at least a part open, A bag having a connecting portion that connects the first compartment and the second compartment was produced (FIG. 1 ).
  • the short side of the first compartment is 70 mm
  • the long side is 90 mm
  • the short side of the second compartment is 70 mm
  • a ⁇ SP having a long side of 75 mm was produced.
  • the protruding portion provided in the first compartment had a short side of 30 mm and a long side of 90 mm.
  • FIG. 9 An actual image of ⁇ SP actually produced in this manner is shown in FIG.
  • the material of ⁇ SP shown in FIG. 9 is a fine fiber sheet made of polyethylene polymer and has the following properties.
  • Mesh average diameter 2 ⁇ m
  • Thickness 75 ⁇ m
  • Chemical resistance No significant change of dissolution, deterioration or discoloration
  • Water permeability Water permeability of 6.5 x 10 -3 cm/s
  • Water retention Water retention rate is 112%
  • Passage of nucleated cells 2 cells or less observed in 10 visual fields in a high-magnification visual field of 200 to 600 times
  • FIG. 9 shows a mode in which the cassette is stored in the second compartment.
  • (B) shows a mode in which the second compartment that encloses the cassette is slid into the first compartment and accommodated.
  • (C) shows a mode in which the heading portion folded back as a lid is folded inward, then both sides are folded inward, and the opening is fixed by a stapler or the like.
  • a sponge sponge was placed in the cassette instead of the tissue section so that the contamination of other cells was easy to see.
  • This cassette was packed with ⁇ SP, and mixed by shaking in a pleural effusion cell suspension fixed with a 10% formalin solution for a whole day and night. Then, the as-packaged cassette was transparentized. After clearing, a separate container containing new paraffin was prepared for each cassette, and the sponge was taken out and embedded in paraffin.
  • a cassette not packed and a cassette packed with a conventional mesh bag were used, and these cassettes were treated in the same manner as above.
  • the completed block was sliced into 4 ⁇ m slices using a new blade and placed on a slide glass for H&E staining. Each block contained 5 sea surface sponges, and the block was deeper cut to make 3 H&E stained specimens.
  • Fig. 10 The results are shown in Fig. 10. As shown in FIG. 10, when the ⁇ SP of the present invention was used, cell contamination could be prevented compared to when it was not used or when compared with a conventional mesh bag. In this experiment, when ⁇ SP was used, contamination of one cell was not observed in all 15 prepared samples. Further, a cell passage test was carried out using the above bag, and the cell images counted in the passage test are shown in FIG. From FIG. 11, it was shown that the sheet using the fine fiber material can almost prevent the passage of cells when it is used in three layers. It was confirmed that the use of prototype bags made of five tissue section packaging materials did not hinder specimen preparation. ⁇ Example 3> In this example, a bag provided with a weight accommodating portion and a heat seal portion was manufactured according to Example 2.
  • FIG. 12A is a diagram in which the weight and the tissue section are accommodated in the compartment
  • FIG. 12B is a diagram in which the compartment is folded.
  • the heat-sealed portion was welded by applying heat of 100°C to 400°C.
  • 1 ⁇ SP of the present invention
  • 10 first compartment
  • 20 second compartment
  • 30 connecting part
  • 40 protruding portion
  • 50a opening portion
  • 50b opening portion
  • 60 adhesion margin
  • 70 tissue section
  • 80 weight mounting portion
  • 81 weight
  • 90 heat seal portion

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
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PCT/JP2019/048574 2018-12-11 2019-12-11 病理組織検体梱包素材及び組織切片のコンタミネーション防止用袋 Ceased WO2020122149A1 (ja)

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JP2020559298A JP7449237B2 (ja) 2018-12-11 2019-12-11 病理組織検体梱包素材及び組織切片のコンタミネーション防止用袋
CN201980081886.8A CN113167692A (zh) 2018-12-11 2019-12-11 病理组织待检体包装材料和组织切片的防污染用袋

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JPS6075301U (ja) * 1983-10-27 1985-05-27 株式会社　石井鉄工所 含水ゴミ収容用袋体
JPH08658U (ja) * 1993-06-02 1996-04-16 義美 巽 生体組織片の収納袋
JP2015506674A (ja) * 2011-12-07 2015-03-05 サイトベラ， インコーポレイテッド 試料処理のための方法および装置
CN202515687U (zh) * 2011-12-14 2012-11-07 复旦大学附属中山医院 一种活检组织收集网袋
JP2018105712A (ja) * 2016-12-26 2018-07-05 学校法人 久留米大学 生体標本作製器具および生体標本作製方法

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