Classifications

• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
  • B01L3/02—Burettes; Pipettes
  • B01L3/0275—Interchangeable or disposable dispensing tips
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
  • B01L2200/02—Adapting objects or devices to another
  • B01L2200/021—Adjust spacings in an array of wells, pipettes or holders, format transfer between arrays of different size or geometry
  • B01L2200/022—Variable spacings
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
  • B01L2200/02—Adapting objects or devices to another
  • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
  • B01L2200/02—Adapting objects or devices to another
  • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
  • B01L2200/06—Fluid handling related problems
  • B01L2200/0689—Sealing
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L2300/00—Additional constructional details
  • B01L2300/06—Auxiliary integrated devices, integrated components
  • B01L2300/0609—Holders integrated in container to position an object
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L2300/00—Additional constructional details
  • B01L2300/08—Geometry, shape and general structure
  • B01L2300/0809—Geometry, shape and general structure rectangular shaped
  • B01L2300/0829—Multi-well plates; Microtitration plates
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L2300/00—Additional constructional details
  • B01L2300/12—Specific details about materials
  • B01L2300/123—Flexible; Elastomeric

Definitions

• the invention relates to a pipetting head for receiving pipette tips, to a pipetting device comprising a pipetting head and to a method for pipetting liquids by means of a pipetting head.
• the ejector can be operated hydraulically or by an electric motor or by means of a pretensioning spring which is tensioned when the pipette tip is placed on the pipetting unit.
• the actuating means for clamping and releasing the pipette tip at the base are complex and take up a lot of space.
• the attachment of the pipette tips in the defined coupling position can easily be prevented by manufacturing tolerances or inaccurate positioning of the pipette tip on the attachment.
• the annular groove and the axial positioning means restrict the use of different pipette tips.
• the approaches are firmly connected to the carrier.
• the clamping forces can be kept low by the two O-rings, so that it is in particular not necessary to support the attachments via springs on the carrier.
• the lugs are supported on the carrier via springs in order to thereby additionally limit the attachment forces.
• the invention relates to a pipetting station or an automatic pipetting device or an automatic laboratory device comprising a pipetting device and / or a transfer device according to claim 12.
• the pipetting station or the automatic pipetting device or the automatic laboratory device can be used for the (automatic) pipetting or transfer of pipettes.
• the laboratory machine can be used for automatic pipetting, for transferring pipette tips and also for other treatments of liquids, for example for tempering, mixing, carrying out chemical or biochemical reactions and other physical, chemical or biochemical treatments.
• a pipette tip is clamped onto each attachment of the pipetting head
• the pipetting head is displaced until each clamped pipette tip has with its lower end contact with a vessel which is provided on a work surface,
• the pipetting head is displaced until the lower end of each clamped pipette tip is at a certain distance from a bottom of the vessel, and
• the pipetting head is shifted until all clamped pipette tips with the lower ends simultaneously come into contact with the Have bottom of a vessel and then the pipetting head is moved until the lower ends of all pipette tips are a certain distance from the bottom of the vessel.
• a particularly small gap can be achieved between the lower ends of the pipette tips clamped in each case and the bottom of the vessels.
• the pipetting head is moved until the lower ends of all clamped pipette tips are in contact with a part of the surface of the vessel other than the bottom. In this way, at least an exact alignment of the pipette tips to the vessels is achieved, only tolerances between the position of the contacted surface and the bottom of the vessel not being compensated for.
• a pipetting head for a pipetting device comprising: a carrier, at least one shoulder held on the carrier for clamping pipette tips and two O-rings held on the shoulder from an elastomer, the two O-rings and the shoulder being formed, a pipette tip alone by clamping the two O-rings on the shoulder.
• Pipetting head according to number 1 or 2 in which the approach has devices for axial position securing, which are designed to support each of the two O-rings in a certain upper and in a certain lower position and a deformation of the two O-rings in the radial direction Allow direction.
• Pipetting head according to number 3 in which the devices for securing the position in the axial direction on the circumference of the attachment are circumferential ring grooves which partially accommodate at least one O-ring or one or more projections on the circumference of the attachment which accommodate at least one O-ring between them.
• Pipetting head according to one of the numbers 1-4 in which the attachment has at least one conical section and / or in which the attachment has at least one cylindrical section.
• Fig. 1 shows a pipetting head with clamped pipette tips in the
• FIG. 2 shows the same pipetting head without pipette tips in the rear view
• Fig. 3 the same pipetting head with clamped pipette tips in one
• Microtiter plate individually set pipette tips in a rough schematic view
• Fig. 9 the pipetting tool with individually adjusted pipette tips withdrawn to a small distance from the bottom area of the microtiter plate in a roughly schematic view.
• the pipetting head 1 has a housing 2, which is formed from a front and a rear housing shell 3, 4, which are assembled in a vertical plane.
• Housing 2 is thus a carrier for the lugs 5.
• each extension 5 has an upper cylindrical section 17 at the top, a lower cylindrical section 18 at the bottom and, in between, a conical section 19 which tapers from top to bottom.
• each cylinder 10 there is a bushing 28, through which a piston 11 is sealingly inserted into the cylinder 10.
• Each piston 11 is designed as a cylindrical rod which is inserted at the top into a central bore 29 of a cylindrical piston head 30 and fastened therein (e.g. glued or pressed in).
• Each piston head 30 has a circumferential annular ring groove 31 on the outer circumference.
• An axially displaceable threaded nut 39 is arranged centrally in the fastening pin 8, the lower end of which is firmly connected to the piston plate 33 in order to displace the piston plate 33 in the axial direction of the cylinder 10.
• the fastening pin 8 has a lower pin section 43 that widens conically downward.
• the lower pin section 43 is firmly connected at its base to the carrier plate 7.
• the driver 54 By turning the driver 54, the spindle 47, which is held axially in the fastening pin 8, moves the threaded nut 39, which is non-rotatably guided in the fastening pin 8, in the axial direction. As a result, the piston plate 33 is displaced and the pistons 11 are displaced in the cylinders 10. By turning the Driver 54 in different directions, the pistons 11 are displaceable in different directions in the cylinders 10. By scanning the position of the cylinder pin 56, it is possible to determine the respective position of the pistons 11 in the cylinders 10.
• “Surface teaching” addresses certain tolerances on the system (e.g. positioning offset and tool tolerance), but is very labor intensive for the user and requires a lot of effort. Furthermore, a large number of tolerances are not covered by surface teaching (e.g. well (well) geometry, microtiter plate seat on adapter, pipette tip geometry, pipette tip seat, tool holder etc.), as this does not take place during the dosing process, but in advance of the application.
• surface teaching e.g. well (well) geometry, microtiter plate seat on adapter, pipette tip geometry, pipette tip seat, tool holder etc.

Landscapes

• Health & Medical Sciences (AREA)
• Clinical Laboratory Science (AREA)
• Chemical & Material Sciences (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Automatic Analysis And Handling Materials Therefor (AREA)
• Devices For Use In Laboratory Experiments (AREA)
• Sampling And Sample Adjustment (AREA)

Priority Applications (6)

Applications Claiming Priority (2)

Publications (1)

Family

ID=64665732

Family Applications (1)

Country Status (7)

Families Citing this family (8)

Citations (14)

Family Cites Families (7)

• 2018
  • 2018-12-14 EP EP18212800.9A patent/EP3666379A1/de not_active Withdrawn
• 2019
  • 2019-12-12 CN CN201980089606.8A patent/CN113396017A/zh active Pending
  • 2019-12-12 AU AU2019398420A patent/AU2019398420A1/en not_active Abandoned
  • 2019-12-12 CA CA3123348A patent/CA3123348A1/en active Pending
  • 2019-12-12 US US17/413,730 patent/US20220097038A1/en active Pending
  • 2019-12-12 EP EP19816770.2A patent/EP3793738A1/de active Pending
  • 2019-12-12 WO PCT/EP2019/084908 patent/WO2020120683A1/de not_active Ceased
  • 2019-12-12 JP JP2021533627A patent/JP7621255B2/ja active Active

Patent Citations (15)

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