WO2020034815A1 - 一种显微授精操作皿 - Google Patents

一种显微授精操作皿 Download PDF

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WO2020034815A1
WO2020034815A1 PCT/CN2019/097386 CN2019097386W WO2020034815A1 WO 2020034815 A1 WO2020034815 A1 WO 2020034815A1 CN 2019097386 W CN2019097386 W CN 2019097386W WO 2020034815 A1 WO2020034815 A1 WO 2020034815A1
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dish
micro
insemination
fluid accumulation
fluid
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PCT/CN2019/097386
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English (en)
French (fr)
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宋宁
吴朝玉
熊小萍
郑众
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杭州德适生物科技有限公司
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Publication of WO2020034815A1 publication Critical patent/WO2020034815A1/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish

Definitions

  • the invention belongs to the field of medical equipment, and particularly relates to a micro-insemination operation dish used in the operation of human sperm injection in oocytes and oocytes.
  • Intracytoplasmic sperm injection in oocytes is a new assisted reproduction technology developed in the 1980s. Its essence is to use a microinjection needle to carry a single sperm directly into the oocyte cytoplasm to achieve fertilization.
  • the micro-insemination operation dish is an important vessel involved in the operation process, including a dish body with an opening facing upwards and a dish lid covering the dish body.
  • the operating fluid into the dish When using, put the operating fluid into the dish first, put the lid on the dish and put it in a constant temperature box at about 37 ° C for preheating and equilibrium for 4 hours to create an insemination environment close to the human environment.
  • the dish was taken out from the incubator and placed on the microscope table.
  • the microscope table has a heating device to maintain the operating temperature of the operation dish at 37 ° C.
  • Sperm and eggs were added to the operating solution, and the eggs were fixed with a microscopic egg holding needle. Sperm is captured with a microinjection needle and injected into the egg.
  • Most of the current micro-insemination operation dishes are circular dishes.
  • the bottom wall of the dishes is set as a whole inclined.
  • This kind of micro-insemination operation dish structure has defects in practical use.
  • the inclined bottom wall cannot fit on the microscope table, which causes the operating solution to be heated unevenly during the insemination operation and may not be maintained at 37 ° C. It is not conducive to fertilization.
  • the round dish takes up a lot of space, and the amount of operating fluid contained in it is also large, and there is waste.
  • the operating fluid is easy to shake out during handling and handling.
  • the invention provides a micro-insemination operation dish, which can effectively ensure that the operation solution is heated uniformly during the insemination process and is maintained at an insemination environment temperature of about 37 ° C. Moreover, the space occupied is small, and the amount of operation solution can be effectively reduced. In order to reduce the waste of operating fluid, in addition, it can also effectively prevent the operating fluid from shaking out from the dish.
  • a micro-insemination operation dish including a dish body with an opening facing upwards and a dish cover covering the dish body, the dish body including a bottom wall and a side wall, wherein The cross section of the dish body is oval.
  • the bottom wall of the dish body is a plate with a uniform thickness.
  • the bottom wall includes a fluid accumulation portion and a raised portion. The fluid accumulation portion is located in the middle of the dish body and the outer wall of the fluid accumulation portion.
  • the raised portions are located on both sides of the fluid accumulation portion, and the outer wall of the raised portion is higher than the bottom edge of the side wall;
  • the raised surface of the raised block is inclined to one side along the length direction of the dish to form an operation surface for insemination operation.
  • the liquid storage space of the dish is stepped, and the fluid accumulation part is the main operation area.
  • the operation fluid is mainly located at the fluid accumulation part.
  • the outer wall of the fluid accumulation part is flush with the bottom of the side wall, so that the dish is placed on the microscope table. After that, the fluid accumulation part can be completely adhered to the microscope table to obtain uniform heat, so that the operation fluid in the operation area is uniformly heated, and is more stably maintained at an insemination environment temperature of about 37 ° C.
  • the microscopic egg holding needles and microinjection needles are arranged left and right along the length of the dish to facilitate operation.
  • the oval dish can reduce the volume of the dish without affecting the injection operation, so that the dish The space occupied is reduced, and the amount of operating fluid contained is also reduced.
  • the setting of the elevated portion can reduce the volume of the operating fluid while further ensuring the depth of the operating fluid in the operating area, further reducing the amount of the operating fluid, and effectively preventing the operating fluid from easily shaking out.
  • a bump with an inclined operating surface is provided on the fluid accumulation part to cooperate with the operation of the microinjection needle, while ensuring the operating area, reducing the processing difficulty of the dish, the structure is simpler, and the bottom of the dish is simpler.
  • the wall also maintains a consistent thickness and is better heated.
  • the side wall of the oval-shaped dish body has a straight wall section, and the straight wall section can be used for attaching signs and labels, making the signs and labels more flat and conducive to writing.
  • the bottom wall includes an inclined connection portion, and the liquid accumulation portion and the elevated portion are connected by a connection portion, and the connection portion gradually slopes upward from the liquid accumulation portion to the elevated portion.
  • the connection part can smoothly connect the effusion part and the raised part, making the bottom wall of the dish easier to shape, and the inclined connection part can prevent the bottom wall of the dish from operating the micro-ovulation needle and the micro-injection needle. Interference to make the insemination operation smoother.
  • connection portion on the horizontal plane is a curved arc shape, and the connection portion is curved toward the fluid accumulation portion.
  • an included angle between the connecting portion and a horizontal plane is ⁇ , where 10 ° ⁇ ⁇ ⁇ 15 °.
  • the angle between the connecting part and the horizontal plane is moderate. If ⁇ ⁇ 10 °, the height difference between the raised part and the effusion part is too small. The effect of reducing the amount of operating fluid is not significant, but it will increase the processing difficulty of the dish. If ⁇ > 15 °, the connection may interfere with the operation of the micro-ovulation needle and the micro-injection needle.
  • the dish is an axisymmetric structure, and the operation surface is located at one side of the midpoint in the length direction of the dish, and is inclined toward the midpoint.
  • the operation surface is actually facing the microinjection needle, and the boss offset to the side of the dish, so that the operation surface is located on the side of the dish, not in the middle, and is reserved for the microinjection needle with complicated operation. More room for operation.
  • one side of the operation surface is connected to the fluid accumulation portion. After the insemination is completed, the fertilized eggs peeled off from the microneedle holding needle can smoothly and smoothly roll down to the effusion section along the operation surface; moreover, one side of the operation surface is connected to the effusion section, making the dish
  • the structure at the bottom is simpler and easier to process.
  • the operation surface is an arc-shaped surface with a middle recessed downward.
  • the tangent angle of the operation surface is 1 ° to 5 °.
  • the area of the operation surface is 0.8 cm 2 to 2 cm 2 .
  • the area of the operation surface is moderate; if the area of the operation surface is less than 0.8 cm 2 , the area of the operation surface is too small, which is not convenient for experimental operation. Fluid accumulation area; if the area of the operation surface is larger than 2cm 2 , the area of the operation surface is too large, the space occupied is large, the operation fluid around the bumps is unevenly heated, and the space occupied by the operation fluid is small, that is, it is used to accommodate eggs , Sperm, fertilized eggs have little space.
  • handles are provided on outer walls of opposite sides of the dish, and two handles are symmetrically arranged along the length direction of the dish. This setting is convenient for the operator to carry and take the dish.
  • Figure 1 is an exploded view of the present invention
  • Figure 2 is a sectional view of a dish body of the present invention
  • Figure 3 is a sectional view of a dish body of the present invention.
  • Figure 4 is a top view of the dish of the present invention.
  • a micro-insemination operation dish shown in FIG. 1 includes a dish body 1 with an opening facing upwards, and a dish cover 2 covering the dish body 1.
  • the cross-section of the dish body 1 is oval.
  • the dish 1 includes a bottom wall 3 and a side wall 4.
  • the bottom wall 3 of the dish 1 is a plate with a uniform thickness, and the bottom wall 3 includes a fluid accumulation portion 31 and a raised portion 32.
  • the fluid accumulation portion 31 is located in the middle of the dish body 1, and the outer wall of the fluid accumulation portion 31 is flush with the bottom edge of the side wall 4.
  • the raised portion 32 is located on both sides of the fluid accumulation portion 31.
  • the outer wall of the elevated portion 32 is higher than the bottom edge of the side wall 4 and forms a height difference with the fluid accumulation portion 31.
  • the fluid accumulation portion 31 is provided with a convex block 5 protruding upward.
  • the top surface of the convex block 5 is inclined to one side along the length direction of the dish 1 to form an operation surface 51 for insemination operation.
  • the liquid must be higher than the bump 5.
  • the bottom wall 3 includes an inclined connection portion 33.
  • the liquid accumulation portion 31 and the elevated portion 32 are connected by the connection portion 33.
  • the connection portion 33 gradually inclines upward from the liquid accumulation portion 31 to the elevated portion 32.
  • connection portion 33 As shown in FIG. 3 and FIG. 4, the projection of the connection portion 33 on the horizontal plane is a curved arc shape, and the connection portion 33 is bent toward the fluid accumulation portion 31.
  • the dish 1 is an axisymmetric structure, and the operation surface 51 is located at one side of the midpoint in the length direction of the dish 1 and is inclined toward the midpoint.
  • the protrusion 5 is disposed on the left side of the center line of the dish 1, and the operation surface 51 faces the right side.
  • One side of the operation surface 51 is connected to the fluid accumulation portion 31.
  • the operation surface 51 is an arc-shaped surface that is depressed downward in the middle.
  • the tangent angle of the operation surface 51 is 1 ° to 5 °. In this embodiment, the tangent angle of the operation surface 51 is 3 °.
  • the area of the operation surface 51 is 0.8 cm 2 to 2 cm 2 . In this embodiment, the area of the operation surface 51 is 1.2 cm 2 .
  • Handles 6 are provided on the outer walls of the opposite sides of the dish 1, and two handles 6 are symmetrically arranged along the length direction of the dish 1.
  • the dish body 1 is an integrally formed structure.
  • the dish 1 is a transparent piece.

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Abstract

本发明公开了一种显微授精操作皿。为克服现有显微授精操作皿中操作液受热不均匀、操作液用量大存在浪费、还容易晃出的缺陷，本发明采用的技术方案包括皿体的横截面呈椭圆形，皿体的底壁为一块厚度一致的板体，包括积液部和抬高部；积液部位于皿体的中部，积液部的外壁与侧壁的底边齐平，抬高部位于积液部的两侧，抬高部的外壁高于侧壁的底边；积液部上设有一块向上凸起的凸块，凸块的顶面倾斜形成用于授精操作的操作面。其优点是：能够有效地保证操作液在授精过程中受热均匀、保持在37℃左右的授精环境温度，而且，占用的空间小，操作液的用量也能够有效地减小，以减少操作液的浪费，此外，还能够有效地避免操作液从皿体中晃出。

Description

一种显微授精操作皿 技术领域
本发明属于医疗设备领域，具体涉及一种在人类辅助生殖医学中，卵母细胞卵胞浆内单精子注射操作时所使用的显微授精操作皿。
背景技术
卵母细胞卵胞浆内单精子注射是20世纪80年代发展的一种新型辅助生殖技术，其实质就是用显微注射针携带单个精子直接注入到卵细胞的卵子胞浆内从而达到受精的目的。显微授精操作皿是该操作过程中涉及的重要器皿，包括开口朝上的皿体和盖在皿体上的皿盖。
使用时，先将操作液放入到皿体内，盖上皿盖后放入到37℃左右的恒温箱内预热平衡4小时，创造出与人体环境接近的授精环境；授精操作时，将操作皿从恒温箱中取出，放置到显微镜台面上，显微镜台面具有加热装置，以对操作皿进行37℃环境温度的维持；在操作液内加入精子和卵子，用显微持卵针固定住卵子，用显微注射针抓取精子并将精子注射到卵子中。
目前的显微授精操作皿大多为圆形的皿体，为了与针尖呈弯折状的显微注射针配合使用，皿体的底壁设置成呈整体倾斜式。这种显微授精操作皿结构在实际使用时存在缺陷：第一，呈倾斜状设置的底壁无法与显微镜台面贴合，导致授精操作过程中，操作液受热不均匀，可能无法保持在37℃，不利于受精；第二，圆形的皿体占用空间大，内部盛装的操作液的用量需求也大，存在浪费；第三，在搬运、拿取过程中，操作液容易晃出。
发明内容
本发明提供一种显微授精操作皿，能够有效地保证操作液在授精过程中受热均匀、保持在37℃左右的授精环境温度，而且，占用的空间小，操作液的用量也能够有效地减少，以减少操作液的浪费，此外，还能够有效地避免操作液从皿体中晃出。
为解决上述技术问题，本发明采用如下技术方案：一种显微授精操作皿，包括开口朝上的皿体和盖在皿体上的皿盖，皿体包括底壁和侧壁，其中，所述皿体的横截面呈椭圆形，皿体的底壁为一块厚度一致的板体，底壁包括积液部和抬高部；所述积液部位于皿体的中部，积液部的外壁与所述侧壁的底边齐平，所述抬高部位于积液部的两侧，抬高部的外壁高于所述侧壁的底边；所述积液部上设有一块向上凸起的凸块，所述凸块的顶面沿皿体的长度方向向一侧倾斜形成用于授精操作的操作面。
皿体的储液空间呈台阶状，积液部为主要的操作区域，操作液主要位于积液部位置，积液部的外壁与侧壁的底边齐平，使得皿体放置到显微镜台面上之后，积液部能够完全与显微镜台面贴合，得到均匀的受热，使操作区域内的操作液受热均匀，更稳定地维持在37℃左右的授精环境温度下。
显微持卵针和显微注射针沿皿体的长度方向一左一右设置，以便操作，椭圆形的皿体能够在不影响注射操作的前提下，减小皿体的体积，使得皿体占用的空间得以减小，盛装的操作液用量也得以减少。而且，抬高部的设置，在保证操作区域内操作液深度的情况下，减少了操作液的体积，进一步地减少了操作液的用量，也能够有效地避免操作液轻易晃出。
此外，在积液部上设置带倾斜的操作面的凸块，用以配合显微注射针的操作，在保证操作面积的同时，降低了皿体的加工难度，结构更加简单，皿体的底壁也能够保持厚度一致，受热效果更好。
再者，椭圆形的皿体的侧壁具有直壁段，直壁段可以用于贴标志、标贴，使得标示、标贴更加平整，也利于书写。
作为改进，所述底壁包括倾斜的连接部，所述积液部和所述抬高部通过连接部连接，连接部自积液部往抬高部逐渐向上倾斜。连接部能够平滑地连接积液部和抬高部，使得皿体的底壁成型更加简单，而且，倾斜的连接部能够避免皿体底壁对显微持卵针和显微注射针操作过程中的干涉，使授精操作更加顺畅。
作为再改进，所述连接部在水平面上的投影呈弯曲的弧形，且连接部朝积液部弯曲。这一设置，使得积液部在底壁上所占的空间小，进一步节省了操作液的用量，而且，弯曲设置的连接部能够适应整体呈椭圆形、两端呈圆形的皿体结构，使得皿体结构强度更高。
作为优选，所述连接部与水平面的夹角为α，其中10°≤α≤15°。连接部与水平面的夹角适中，若α＜10°，抬高部与积液部的高度差过小，减少操作液用量的效果并不显著，反而徒增皿体的加工难度；若α＞15°，连接部可能会干涉到显微持卵针和显微注射针的操作。
作为改进，所述皿体为轴对称式结构，所述操作面位于皿体长度方向上的中点的一侧，并朝向中点倾斜。授精操作时，操作面实际是朝向显微注射针的，而往皿体一侧偏置的凸台，使得操作面位于皿体的一侧，而非正中，为操作复杂的显微注射针留出更多的操作空间。
作为改进，所述操作面的一侧边与所述积液部相连。完成授精后，从显微持卵针上剥离下来的受精卵能够顺着操作面顺利、平稳地滚落到积液部上；而且，操作面的一侧边与 积液部相连，使得皿体底部的结构更加简单，加工方便。
作为改进，所述操作面为中部向下凹陷的弧形面。完成授精后，从显微持卵针上剥离下来的受精卵能够更好、更稳地被向下凹陷的弧形面承托住，也利于受精卵缓慢滚落。
作为优选，所述操作面的切线角度为1°～5°。
作为优选，所述操作面的面积为0.8cm 2～2cm 2。操作面的面积大小适中；若操作面的面积小于0.8cm 2，操作面面积过小，不便于实验操作，完成授精后的受精卵从显微持卵针上剥离后也容易偏离直接掉落到积液部；若操作面的面积大于2cm 2，操作面面积过大，所占的空间大，凸块周围的操作液受热不均匀，操作液所占空间小，也就是说，用于容纳卵子、精子、受精卵的空间小。
作为改进，所述皿体的相对两侧的外壁上设有提手，两个提手沿皿体的长度方向对称设置。这一设置，便于操作者搬运、拿取皿体。
附图说明
图1为本发明的爆炸图；
图2为本发明皿体的剖视图；
图3为本发明皿体的剖视图；
图4为本发明皿体的俯视图；
图中所示：1、皿体；2、皿盖；3、底壁；31、积液部；32、抬高部；33、连接部；4、侧壁；5、凸块；51、操作面；6、提手。
具体实施方式
下面结合本发明实施例的附图，对本发明实施例的技术方案进行解释和说明，但下述实施例仅为本发明的优选实施例，并非全部。基于实施方式中的实施例，本领域技术人员在没有做出创造性劳动的前提下所获得的其他实施例，都属于本发明的保护范围。
如图1所示的一种显微授精操作皿，包括开口朝上的皿体1和盖在皿体1上的皿盖2，所述皿体1的横截面呈椭圆形。
结合图2、图3，皿体1包括底壁3和侧壁4，皿体1的底壁3为一块厚度一致的板体，底壁3包括积液部31和抬高部32。所述积液部31位于皿体1的中部，积液部31的外壁与所述侧壁4的底边齐平。所述抬高部32位于积液部31的两侧，抬高部32的外壁高于所述侧壁4的底边，与积液部31形成高度差。
所述积液部31上设有一块向上凸起的凸块5，所述凸块5的顶面沿皿体1的长度方向向一侧倾斜形成用于授精操作的操作面51，加入的操作液必须高于凸块5。
所述底壁3包括倾斜的连接部33，所述积液部31和所述抬高部32通过连接部33连接，连接部33自积液部31往抬高部32逐渐向上倾斜。
所述连接部33与水平面的夹角为α，其中10°≤α≤15°。在本实施例中，α＝12°。
如图3、图4所示，所述连接部33在水平面上的投影呈弯曲的弧形，且连接部33朝积液部31弯曲。
所述皿体1为轴对称式结构，所述操作面51位于皿体1长度方向上的中点的一侧，并朝向中点倾斜。具体在本例中，所述凸块5设置在皿体1的中线的左侧，所述操作面51朝向右侧。
所述操作面51的一侧边与所述积液部31相连。
所述操作面51为中部向下凹陷的弧形面。
所述操作面51的切线角度为1°～5°。在本实施例中，所述操作面51的切线角度为3°。
所述操作面51的面积为0.8cm 2～2cm 2。在本实施例中，所述操作面51的面积为1.2cm 2。
所述皿体1的相对两侧的外壁上设有提手6，两个提手6沿皿体1的长度方向对称设置。
所述皿体1为一体成形结构。
所述皿体1为透明件。
以上所述，仅为本发明的具体实施方式，但本发明的保护范围并不局限于此，熟悉该本领域的技术人员应该明白本发明包括但不限于附图和上面具体实施方式中描述的内容。任何不偏离本发明的功能和结构原理的修改都将包括在权利要求书的范围中。

Claims (10)

  1. 一种显微授精操作皿，包括开口朝上的皿体和盖在皿体上的皿盖，皿体包括底壁和侧壁，其特征在于：所述皿体的横截面呈椭圆形，皿体的底壁为一块厚度一致的板体，底壁包括积液部和抬高部；所述积液部位于皿体的中部，积液部的外壁与所述侧壁的底边齐平，所述抬高部位于积液部的两侧，抬高部的外壁高于所述侧壁的底边；所述积液部上设有一块向上凸起的凸块，所述凸块的顶面沿皿体的长度方向向一侧倾斜形成用于授精操作的操作面。
  2. 根据权利要求1所述的一种显微授精操作皿，其特征在于：所述底壁包括倾斜的连接部，所述积液部和所述抬高部通过连接部连接，连接部自积液部往抬高部逐渐向上倾斜。
  3. 根据权利要求1所述的一种显微授精操作皿，其特征在于：所述连接部在水平面上的投影呈弯曲的弧形，且连接部朝积液部弯曲。
  4. 根据权利要求2所述的一种显微授精操作皿，其特征在于：所述连接部与水平面的夹角为α，其中10°≤α≤15°。
  5. 根据权利要求1所述的一种显微授精操作皿，其特征在于：所述皿体为轴对称式结构，所述操作面位于皿体长度方向上的中点的一侧，并朝向中点倾斜。
  6. 根据权利要求1所述的一种显微授精操作皿，其特征在于：所述操作面的一侧边与所述积液部相连。
  7. 根据权利要求1所述的一种显微授精操作皿，其特征在于：所述操作面为中部向下凹陷的弧形面。
  8. 根据权利要求7所述的一种显微授精操作皿，其特征在于：所述操作面的切线角度为1°～5°。
  9. 根据权利要求1所述的一种显微授精操作皿，其特征在于：所述操作面的面积为0.8cm 2～2cm 2。
  10. 根据权利要求1所述的一种显微授精操作皿，其特征在于：所述皿体的相对两侧的外壁上设有提手，两个提手沿皿体的长度方向对称设置。
PCT/CN2019/097386 2018-08-16 2019-07-24 一种显微授精操作皿 WO2020034815A1 (zh)

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