WO2013110237A1 - 一种培养皿 - Google Patents

一种培养皿 Download PDF

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Publication number
WO2013110237A1
WO2013110237A1 PCT/CN2013/070858 CN2013070858W WO2013110237A1 WO 2013110237 A1 WO2013110237 A1 WO 2013110237A1 CN 2013070858 W CN2013070858 W CN 2013070858W WO 2013110237 A1 WO2013110237 A1 WO 2013110237A1
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Prior art keywords
dish
side wall
culture
bottom wall
secondary side
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PCT/CN2013/070858
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English (en)
French (fr)
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彭建军
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Peng Jianjun
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Publication of WO2013110237A1 publication Critical patent/WO2013110237A1/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions

Definitions

  • the utility model relates to the field of cell culture vessels, which is a petri dish used for the manipulation and culture of gametes and embryos in human assisted reproductive technology.
  • the culture dish As a cell culture vessel, the culture dish is widely used in biological fields such as bacteria and cell culture.
  • the main structure of a common petri dish includes a dish body and a dish cover, which are integrally formed by a bottom wall and a side wall, respectively.
  • Existing culture dishes are basically universal for different culture objects. The general design is often at the expense of individualized needs. When such universal culture dishes are applied to some special culture objects, the individualized requirements cannot be fully satisfied, showing the shortage of universal culture dishes. Therefore, for some special operation objects and culture objects, it is necessary to design special culture dishes for their particularity to suit their individual needs.
  • Gametes and embryos in the treatment of human assisted reproductive technology are a special type of manipulation and culture objects.
  • the particularity of human gamete and embryo manipulation and culture is mainly manifested in three aspects: First, the use of liquid medium and liquid mineral oil.
  • the liquid medium droplets are dripped on the inner surface of the bottom wall of the culture dish, and then covered with mineral oil which is sufficiently balanced by the gas in the CO2 incubator.
  • Gametes and embryos at different stages require a suitable gas phase culture environment.
  • the gas phase in the liquid medium is required to be well balanced with the gas phase in the CO2 incubator.
  • the wide mineral oil interface does not interfere with the balance between the medium and the CO2 incubator gas phase. .
  • the transfer of the culture tray from the preparation of the culture tray to the gamete operation, embryo culture, embryo transfer, embryo freezing, etc. can not completely avoid the mineral oil overflow caused by the unevenness of the culture dish end.
  • the overflow of mineral oil causes the following problems: the spilled mineral oil is dirty on the outside of the culture dish and the table on which the culture dish is placed, including the heating table, the microscope heating plate, the lens and the CO2 incubator partition, which affects the smooth operation of each operation line;
  • the overflowing mineral oil has the risk of backflow after contacting the side wall of the dish; when the overflowing mineral oil is too much, the gas permeable gap of the culture dish is blocked by mineral oil, which is not conducive to the balance between the medium and the gas phase of the CO2 incubator.
  • the heating plate (stage) sets a temperature suitable for the gametes and the embryos.
  • the temperature of the medium in the culture dish will be close to the set temperature only when the bottom of the culture dish is sufficiently contacted with the heating plate (stage).
  • the particularity of human gamete and embryo manipulation and culture requires a more appropriate design of the culture dish to meet its specific needs.
  • the smooth operation of human gametes and embryos and the proper gas phase and temperature when operating outside the CO2 incubator are related to the quality of the embryos, which directly affects the success or failure of the treatment.
  • a dish and a lid which are integrally formed by a bottom wall and a side wall;
  • the space formed by the wall and the side wall is a culture space, and the dish cover functions to cover the culture space of the dish body;
  • the inner surface of the bottom wall of the dish cover has three triangular ribs distributed in an equilateral triangle, when the lid is fastened to the dish body
  • the inner surface of the bottom wall of the dish cover is separated from the top end of the side wall of the dish body to form a gas permeable slit;
  • a support ring is arranged on the outer periphery of the bottom wall of the dish body, and the bottom wall of the dish body is supported away from the loading table.
  • the purpose of the utility model is to solve the deficiencies in the operation and culture application of gametes and embryos in the human assisted reproductive technology, and to provide a special culture dish which is more suitable for the special needs of human gametes and embryos.
  • a petri dish for the manipulation and culture of gametes and embryos in human assisted reproductive technology including a dish body and a dish cover.
  • the dish body comprises a bottom wall of the dish body, a main side wall and a secondary side wall, and the three parts are integrally formed.
  • the top end of the main side wall is higher than the top end of the sub-side wall, the vertical spacing is 3mm-5mm; the horizontal distance between the outer edge of the top side end of the sub-side wall is 3mm-5mm from the inner surface of the main side wall; the main side wall
  • the inner surface, the outer surface of the secondary side wall and a portion of the inner surface of the bottom wall of the dish body form an annular groove; the annular groove is for accommodating overflowing mineral oil; and the secondary side wall is formed with a cylinder formed by the bottom wall of the dish body
  • the shaped space constitutes a culture space; the inner surface of the secondary side wall constituting the culture space, and the inner surface of the bottom wall of the dish body are treated by TC; the outer surface of the bottom wall of the dish body of the culture space can be combined with a heating plate (table) panel Closely fit.
  • the culture dish of the present invention is further configured such that the dish body and the dish cover have a cylindrical shape.
  • the culture dish of the present invention is further configured such that the culture dish is made of an organic polymer material such as polystyrene.
  • the culture dish of the present invention is further configured such that the culture dish is colorless and transparent.
  • the culture dish of the present invention is further configured to: the inner surface of the secondary side wall constituting the culture space, and the inner surface of the bottom wall of the dish body are subjected to TC treatment.
  • the culture dish of the present invention is further configured such that the bottom wall of the dish body, the main side wall and the secondary side wall have a thickness of about 1 mm.
  • the culture dish of the present invention is further provided such that the main side wall is a cylindrical structure formed by extending outward from the outer circumference of the bottom wall of the dish body centering on the center of the bottom wall of the dish body.
  • the culture dish of the present invention is further configured such that the main sidewall has an upward abduction angle of 0 degrees to 5 degrees.
  • the culture dish of the present invention is further configured such that the vertical height of the main side wall is 8 mm to 18 mm.
  • the culture dish of the present invention is further configured such that the secondary side wall is a cylindrical structure formed by extending outward from the inner surface of the bottom wall of the dish body centering on the center of the bottom wall of the dish body.
  • the culture dish of the present invention is further configured such that the secondary sidewall has an upward abduction angle of 0 degrees to 5 degrees.
  • the culture dish of the present invention is further configured such that the secondary side wall has a vertical height of 5 mm to 15 mm.
  • the culture dish of the present invention is further configured such that the dish cover comprises a bottom wall of the dish cover and a side wall of the dish cover, and the two parts are integrally formed.
  • the culture dish of the present invention is further configured such that the bottom wall of the dish cover and the side wall of the dish cover have a thickness of about 1 mm.
  • the culture dish of the utility model is further arranged such that the inner surface diameter of the bottom wall of the dish cover is larger than the outer diameter of the upper edge of the main side wall of the dish body, and the diameter difference is about 1.0 mm-1.5 mm.
  • the culture dish of the utility model is further arranged such that: three inner ribs arranged in a triangular shape in the inner surface of the bottom wall of the dish cover have a height of about 0.4 mm, a width of about 1 mm, a length of about 2 mm, and a centrifugal end.
  • the inner surface of the side wall of the dish cover has a height of about 0.4 mm, a width of about 1 mm, a length of about 2 mm, and a centrifugal end.
  • the petri dish of the present invention is further configured such that the rib is supported on the upper edge of the main side wall of the dish after the lid is fastened to the dish, so that the inner surface of the bottom wall of the dish is A gas permeable gap is formed between the upper edges of the main sidewalls of the dish.
  • the petri dish of the present invention is further arranged such that the outer surface of the bottom wall of the dish cover is recessed downward with the outer diameter of the bottom wall of the dish body as a center around the center of the bottom wall of the dish cover to form a cylindrical stacking groove.
  • the culture dish of the present invention is further arranged such that the depth of the stacking groove is about 0.5 mm.
  • the culture dish of the present invention is further configured such that the side wall of the dish cover is a cylindrical structure formed by extending outward from the center of the bottom wall of the dish cover with the center of the bottom wall of the dish cover as a center.
  • the culture dish of the utility model is further arranged such that the sidewall of the dish cover has a downward abduction angle of 0 to 5 degrees.
  • the culture dish of the present invention is further arranged such that the vertical height of the side wall of the dish cover is 50%-75% of the vertical height of the main side wall of the dish body.
  • the utility model has the following beneficial effects: First, the utility model is used as a gamete and embryo operation and culture in the human assisted reproductive technology, and the spilled material is stored when the mineral oil overflows due to the unevenness of the culture dish end. In the annular groove, various problems caused by the overflowing mineral oil are avoided; second, the outer wall of the dish body corresponding to the outer space of the culture space can be closely adhered to the heating plate (table) panel, which is favorable for heat conduction, and the temperature can be compensated in time and reduced. Risk of damage to gametes and embryos.
  • Figure 1 is a perspective view of the petri dish cover body of the present invention.
  • Figure 2 is a longitudinal sectional view of the culture vessel dish cover body of the present invention.
  • Figure 3 is a perspective cross-sectional view of the culture vessel cover of the present invention.
  • Dish cover 21 The bottom wall of the lid 22. The side wall of the lid 23. Stacking groove 24. A rib.
  • the present invention is a petri dish for the manipulation and culture of gametes and embryos in human assisted reproductive technology, which is composed of a dish 1 and a lid 2.
  • the culture dish is made of an organic polymer material, such as polystyrene, and is colorless and transparent; the dish 1 and the dish cover 2 have a cylindrical shape.
  • the dish body 1 includes a bottom wall 11 of the dish body, a main side wall 12 and a secondary side wall 13, and the three portions are integrally formed; the thickness of the bottom wall 11, the main side wall 12 and the second side wall 13 of the dish body About 1 mm; the outer surface of the bottom wall 11 of the culture chamber 15 can be closely adhered to the heating plate (table) panel; the vertical height of the main sidewall 12 is 8 mm-18 mm, and the upward abduction angle is 0. The height of the main side wall 12 is higher than the top end of the secondary side wall 13 with a vertical spacing of 3 mm to 5 mm.
  • the height difference is sufficient to ensure that the inner surface of the bottom wall 21 of the lid is not touched when the mineral oil overflows over the top end of the secondary side wall 13; the horizontal distance of the outer edge of the top end of the secondary side wall 13 from the inner surface of the main side wall 12 is 3mm-5mm.
  • the spacing is sufficient to ensure that the upper edge of the main sidewall 12 is not touched when the mineral oil overflows over the top end of the secondary sidewall 13; the inner surface of the main sidewall 12, the outer surface of the secondary sidewall 13 and a portion of the bottom of the vessel
  • the inner surface of the wall 11 encloses an annular groove 14; the annular groove 14 is for accommodating overflowing mineral oil; the cylindrical space formed by the secondary side wall 13 and the bottom wall 11 of the dish constitutes a culture space 15;
  • the inner surface of the secondary side wall 13 of the culture space 15 and the inner surface of the bottom wall 11 of the dish body are subjected to TC treatment.
  • the dish cover 2 comprises a bottom cover wall 21 and a side wall 22 of the dish cover, the two parts are integrally formed; the bottom wall 21 of the dish cover and the side wall 22 of the dish cover have a thickness of about 1 mm;
  • the inner diameter of 21 is larger than the outer diameter of the upper edge of the main side wall 12, and the diameter difference is about 1.0 mm-1.5 mm;
  • the inner surface of the bottom wall 21 of the dish cover is a triangular rib 24 arranged in a triangular shape, and the height is about 0.4 mm, a width of about 1 mm, a length of about 2 mm, the centrifugal end of which terminates in the inner surface of the side wall of the dish cover;
  • the rib 24 is attached to the dish 1 after the dish cover 2 is fastened
  • the upper edge of the main sidewall 12 forms a gas permeable gap between the inner surface of the bottom wall 21 of the lid and the upper edge of the main sidewall 12; the outer surface of the bottom wall 21 of the lid is

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Abstract

一种用于人类辅助生殖技术中配子和胚胎操作及培养的培养皿，包括皿体和皿盖。皿体包括皿体底壁、主侧壁和次侧壁，三个部分一体成型；所述主侧壁顶端高于所述次侧壁顶端，垂直间距为3.0-5.0mm；次侧壁顶端外缘距所述主侧壁内面的水平间距为3.0-5.0mm；所述主侧壁内面、所述次侧壁外面与部分所述皿体底壁内面围成环形槽；所述次侧壁与所述皿体底壁形成的圆筒形空间构成培养空间；所述皿体底壁对应培养空间的外面能够与加热板面板紧密贴合。该培养皿能防止溢出矿物油，利于热传导及时补偿温度。

Description

一种培养皿
技术领域
本实用新型涉及细胞培养器皿领域，为一种用于人类辅助生殖技术中配子和胚胎操作及培养的培养皿。
背景技术
培养皿作为一种细胞培养器皿，广泛应用于细菌、细胞培养等生物学领域。普通培养皿主要结构包括一个皿体和一个皿盖，分别由一个底壁和一个侧壁一体成型。现有的培养皿针对不同培养对象基本上都可以通用。通用的设计往往是以牺牲个性化需求为代价，当这类通用培养皿应用于一些特殊培养对象时，个性化需求就不能得到充分满足，显示出通用培养皿的不足。因此对一些特殊操作对象及培养对象，有必要针对其特殊性设计特殊的培养皿，以适应其个性化需求。
人类辅助生殖技术治疗中配子和胚胎就是一类特殊的操作对象及培养对象。人类配子和胚胎操作及培养的特殊性主要表现在三方面：一，使用液体培养基和液态矿物油。液体培养基微滴滴在培养皿体底壁内面，再用经过CO2培养箱中气体充分平衡的矿物油覆盖。配子及不同阶段的胚胎需要适当的气相培养环境，要求液体培养基内的气相成份能够与CO2培养箱内的气相充分平衡，宽大的矿物油界面不妨碍培养基与CO2培养箱气相之间的平衡。二，从培养盘的配制到配子操作、胚胎培养、胚胎移植、胚胎冷冻等多个步骤对培养盘进行转移，不能完全避免因培养皿端持不平而导致矿物油溢出。矿物油溢出引发如下问题：溢出的矿物油脏污培养皿皿体外面及放置培养皿的台面，包括加热台、显微镜加热板、镜头和CO2培养箱隔板，影响各项操作行的顺利进行；溢出的矿物油在接触到皿体侧壁外面后有回流引起污染的风险；溢出的矿物油过多时，培养皿透气缝隙被矿物油封闭，不利于培养基与CO2培养箱气相之间的平衡。三，对配子及胚胎的操作需要在温度适当的加热板（台）上进行，目的是避免温度降低对配子及胚胎造成损伤。加热板（台）设定一个适合配子及胚胎的温度，只有在培养皿皿底充分接触到加热板（台）时，培养皿内培养基的温度才会与设定的温度接近。人类配子和胚胎操作及培养的特殊性要求对培养皿做出更恰当的设计以满足其特殊需要。对人类配子和胚胎操作顺利与否及CO2培养箱外操作时气相和温度适当与否关系到胚胎质量的好坏，进而直接影响到助孕治疗的成败。
目前，在人类辅助生殖技术中配子和胚胎操作及培养中最常用的培养皿的主要结构特征为：一个皿体和一个皿盖，分别由一个底壁和一个侧壁一体成型；皿体的底壁和侧壁构成的空间为培养空间，皿盖起到遮盖皿体培养空间的作用；皿盖底壁内面呈等三角分布的三个一字凸棱，在皿盖扣合到皿体上时，将皿盖底壁内面和皿体侧壁顶端隔开，形成透气缝隙；皿体底壁外面外周有一支撑环，将皿体底壁托离载物台面。
在实践中发现，此类设计的培养皿不能充分满足人类辅助生殖技术中配子和胚胎操作及培养的特殊性，存在以下两方面的缺陷：一，在矿物油溢出时，溢出的矿物油所引发的各类问题不能避免；二，支撑环使皿体底壁托离加热板（台）面板而不能紧密贴合，不利于热传导，存在因温度不能及时补偿而损伤配子及胚胎的风险。
发明内容
实用新型目的
本实用新型的目的是解决普通培养皿在人类辅助生殖技术中配子和胚胎操作及培养应用中的不足，提供一种更适合人类配子和胚胎操作及培养特殊需要的专用培养皿。
技术方案
为实现上述目的，本实用新型采用以下技术方案：一种用于人类辅助生殖技术中配子和胚胎操作及培养的培养皿，包括皿体和皿盖。其中皿体包括皿体底壁、主侧壁和次侧壁，三个部分一体成型。所述主侧壁顶端高于所述次侧壁顶端，垂直间距为3mm-5mm；所述次侧壁顶端外缘距所述主侧壁内面的水平间距为3mm-5mm；所述主侧壁内面、所述次侧壁外面与部分所述皿体底壁内面围成环形槽；所述环形槽用于收纳溢出的矿物油；所述次侧壁与所述皿体底壁形成的圆筒形空间构成培养空间；构成所述培养空间的所述次侧壁内面、所述皿体底壁内面经TC处理；所述培养空间的所述皿体底壁外面能够与加热板（台）面板紧密贴合。
本实用新型的培养皿进一步设置为：所述皿体和所述皿盖呈圆筒形。
本实用新型的培养皿进一步设置为：所述培养皿采用有机高分子材料制成，如聚苯乙烯等。
本实用新型的培养皿进一步设置为：所述培养皿无色透明。
本实用新型的培养皿进一步设置为：构成所述培养空间的所述次侧壁内面、所述皿体底壁内面经TC处理。
本实用新型的培养皿进一步设置为：所述皿体底壁、所述主侧壁和所述次侧壁厚度约为1mm。
本实用新型的培养皿进一步设置为：所述主侧壁为自所述皿体底壁外周以所述皿体底壁圆心为中心向上外展延伸形成的圆筒状结构。
本实用新型的培养皿进一步设置为：所述主侧壁向上外展角度为0度-5度。
本实用新型的培养皿进一步设置为：所述主侧壁的垂直高度为8mm-18mm。
本实用新型的培养皿进一步设置为：所述次侧壁为自所述皿体底壁内面以所述皿体底壁圆心为中心向上外展延伸形成的圆筒状结构。
本实用新型的培养皿进一步设置为：所述次侧壁向上外展角度为0度-5度。
本实用新型的培养皿进一步设置为：所述次侧壁的垂直高度为5mm-15mm。
本实用新型的培养皿进一步设置为：所述皿盖包括皿盖底壁和皿盖侧壁，两个部分一体成型。
本实用新型的培养皿进一步设置为：所述皿盖底壁和所述皿盖侧壁厚度约为1mm。
本实用新型的培养皿进一步设置为：所述皿盖底壁内面直径大于所述皿体主侧壁上缘外直径，直径差约为1.0mm-1.5mm。
本实用新型的培养皿进一步设置为：所述皿盖底壁内面三条呈等三角辐射状排列的一字凸棱，高度约为0.4mm，宽度约为1mm，长度约为2mm，其离心端止于所述皿盖侧壁内面。
本实用新型的培养皿进一步设置为：所述一字凸棱在所述皿盖扣合到所述皿体后架在所述皿体主侧壁上缘，致所述皿盖底壁内面与所述皿体主侧壁上缘之间形成透气缝隙。
本实用新型的培养皿进一步设置为：所述皿盖底壁外面以所述皿盖底壁圆心为中心以所述皿体底壁外面直径为直径向下凹陷形成圆筒形堆叠槽。
本实用新型的培养皿进一步设置为：所述堆叠槽的深度约为0.5mm。
本实用新型的培养皿进一步设置为：所述皿盖侧壁为自所述皿盖底壁外周以所述皿盖底壁圆心为中心向下外展延伸形成的圆筒状结构。
本实用新型的培养皿进一步设置为：所述皿盖侧壁向下外展角度为0度-5度。
本实用新型的培养皿进一步设置为：所述皿盖侧壁的垂直高度为所述皿体主侧壁垂直高度的50%-75%。
有益效果
与现有技术相比，本实用新型具有如下有益效果：一，本实用新型用作人类辅助生殖技术中配子和胚胎操作及培养，在矿物油因培养皿端持不平而溢出时，溢出物收纳在环形槽内，避免了溢出的矿物油所引发的各类问题；二，皿体底壁对应培养空间的外面能够与加热板（台）面板紧密贴合，利于热传导，温度能及时补偿而降低损伤配子及胚胎的风险。
附图说明
图1是本实用新型培养皿皿盖皿体立体透视图；
图2是本实用新型培养皿皿盖皿体纵向剖视图；
图3是本实用新型培养皿皿体皿盖立体剖视图。
图示说明：1.皿体 11.皿体底壁 12.主侧壁 13.次侧壁 14.环形槽 15.培养空间2.皿盖 21.皿盖底壁 22.皿盖侧壁 23.堆叠槽24.一字凸棱。
具体实施方式
参阅说明书附图1和附图2所示，本实用新型为一种用于人类辅助生殖技术中配子和胚胎操作及培养的培养皿，由皿体1和皿盖2两部分组成。所述培养皿采用有机高分子材料制成，如聚苯乙烯等，无色透明；所述皿体1和所述皿盖2呈圆筒形。
所述皿体1包括皿体底壁11、主侧壁12和次侧壁13，三个部分一体成型；所述皿体底壁11、所述主侧壁12和所述次侧壁13厚度约为1mm；所述培养空间15的所述皿体底壁11外面能够与加热板（台）面板紧密贴合；所述主侧壁12的垂直高度为8mm-18mm，向上外展角度为0度-5度；所述主侧壁12顶端高于所述次侧壁13顶端，垂直间距为3mm-5mm。经实验，本高度差足够保证在矿物油溢出越过次侧壁13顶端时不会触及皿盖底壁21内面；所述次侧壁13顶端外缘距所述主侧壁12内面的水平间距为3mm-5mm。经实验，本间距足够保证在矿物油溢出越过次侧壁13顶端时不会触及主侧壁12上缘；所述主侧壁12内面、所述次侧壁13外面与部分所述皿体底壁11内面围成环形槽14；所述环形槽14用于收纳溢出的矿物油；所述次侧壁13与所述皿体底壁11形成的圆筒形空间构成培养空间15；构成所述培养空间15的所述次侧壁13内面、所述皿体底壁11内面经TC处理。
所述皿盖2包括皿盖底壁21和皿盖侧壁22，两个部分一体成型；所述皿盖底壁21和所述皿盖侧壁22厚度约为1mm；所述皿盖底壁21内面直径大于所述主侧壁12上缘外直径，直径差约为1.0mm-1.5mm；所述皿盖底壁21内面三条呈等三角辐射状排列的一字凸棱24，高度约为0.4mm，宽度约为1mm，长度约为2mm，其离心端止于所述皿盖侧壁内面；所述一字凸棱24在所述皿盖2扣合到所述皿体1后架在所述主侧壁12上缘，使所述皿盖底壁21内面与所述主侧壁12上缘之间形成透气缝隙；所述皿盖底壁21外面以所述皿盖底壁21圆心为中心以所述皿体底壁11外面直径为直径向下凹陷形成圆筒形堆叠槽23；所述堆叠槽23的深度约为0.5mm；所述皿盖侧壁22向下外展角度为0度-5度；所述皿盖侧壁22的垂直高度为所述主侧壁12垂直高度的50%-75%。多个所述培养皿正向堆叠时，上方一只所述培养皿的皿体底壁11可以嵌合到下方一只所述培养皿的堆叠槽23内，防止转移时滑落。
上述具体实施方式仅为本创作的较佳实施例，并不用以限制本创作，凡在本创作的精神及原则之内所做的任何修改、等同替换、改进等，均应包含在本创作的保护范围之内。

Claims (1)

  1. 1. 本实用新型为一种用于人类辅助生殖技术中配子和胚胎操作及培养的培养皿，包括皿体和皿盖，其特征在于：所述皿体包括皿体底壁、主侧壁和次侧壁，三个部分一体成型；所述次侧壁与所述皿体底壁形成的圆筒形空间构成培养空间；所述培养空间的所述皿体底壁外面能够与加热板面板紧密贴合。
    2. 如权利要求1所述的培养皿，其特征为：所述主侧壁向上外展角度为0度-5度。
    3. 如权利要求1所述的培养皿，其特征为：所述次侧壁为自所述皿体底壁内面以所述皿体底壁圆心为中心向上外展延伸形成的圆筒状结构。
    4. 如权利要求3所述的次侧壁，其特征为：所述次侧壁向上外展角度为0度-5度。
    5. 如权利要求3所述的次侧壁，其特征为：所述次侧壁的垂直高度为5.0mm-15.0mm。
    6. 如权利要求1所述的培养皿，其特征为：所述主侧壁顶端高于所述次侧壁顶端，垂直间距为3.0mm-5.0mm。
    7. 如权利要求1所述的培养皿，其特征为：所述次侧壁顶端外缘距所述主侧壁内面的水平间距为3.0mm-5.0mm。
    8. 如权利要求1所述的培养皿，其特征为：所述主侧壁、所述次侧壁与两者之间的所述皿体底壁围成环形槽。
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CN107723237A (zh) * 2017-11-23 2018-02-23 北京大学深圳医院 辅助生殖试验用多用途培养皿
CN108893403A (zh) * 2018-09-27 2018-11-27 张树军 立式培养皿及其系统和方法
CN110093273A (zh) * 2019-06-13 2019-08-06 中国科学院苏州生物医学工程技术研究所 胚胎时差成像培养模块

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CN104152352A (zh) * 2014-08-04 2014-11-19 佘力平 细胞培养室及其配套之加热套与支座
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CN108893403A (zh) * 2018-09-27 2018-11-27 张树军 立式培养皿及其系统和方法
CN110093273A (zh) * 2019-06-13 2019-08-06 中国科学院苏州生物医学工程技术研究所 胚胎时差成像培养模块
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