WO2019139214A1 - Composition cosmétique contenant un algasome comprenant, au sein de ce dernier, un extrait de micro-algues - Google Patents

Composition cosmétique contenant un algasome comprenant, au sein de ce dernier, un extrait de micro-algues Download PDF

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Publication number
WO2019139214A1
WO2019139214A1 PCT/KR2018/009805 KR2018009805W WO2019139214A1 WO 2019139214 A1 WO2019139214 A1 WO 2019139214A1 KR 2018009805 W KR2018009805 W KR 2018009805W WO 2019139214 A1 WO2019139214 A1 WO 2019139214A1
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skin
weight
microalgae extract
cosmetic composition
algae
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PCT/KR2018/009805
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English (en)
Korean (ko)
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김차중
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(주)알렌바이오
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/14—Liposomes; Vesicles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/68—Sphingolipids, e.g. ceramides, cerebrosides, gangliosides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9706—Algae
    • A61K8/9722—Chlorophycota or Chlorophyta [green algae], e.g. Chlorella
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations

Definitions

  • the present invention relates to a cosmetic composition containing an algae containing a microalgae extract.
  • the skin cells are active physiologically.
  • synthesis and metabolism of collagen important for suppressing the elasticity and wrinkle formation of the skin are active, and also, it is necessary for the skin including the hyaluronic acid GAGS (Glucosaminoglycans), a protein, is also actively synthesized, which can impart elasticity, softness, flexibility and moisturizing properties to the skin.
  • GAGS Glucosaminoglycans
  • proliferation of basal layer cells is active, and the production of binding proteins such as laminin, integrin, and desmosomes that enhance binding between skin cells is balanced Strengthens skin tissue to maintain healthy skin.
  • reactive oxygen radicals and free radicals which are generated due to increase of ultraviolet ray amount, air pollution and excessive fatigue and stress of modern people, oxidize or denature biological components (protein, nucleic acid, cell membrane lipid, etc.)
  • free radicals As the main cause of the rise, interest in free radicals is increasing.
  • the biosynthesis of various biologic antioxidants decreases, and oxidative stress due to various radicals is exposed to the harmful environment such as pollution of the atmospheric environment, unhealthy diet, ultraviolet ray exposure, stress and disease It becomes difficult to erase sufficiently, and when exposed to such oxidative stress, the skin ages and changes variously such as wrinkles, decreased elasticity, stain, freckles, and drying.
  • U.S. Patent Nos. 4603146 and 4877805 disclose examples using retinoids (vitamin A).
  • retinoids vitamin A
  • retinol is very limited in the cosmetic field due to the problem that it is discolored, detached, and titered because it is prescribed to the product and its effect is decreased and skin irritation is caused despite the use of a small amount.
  • U.S. Patent No. 3,906,108 discloses an oil-in-water (O / W) emulsifier type in which retinoic acid is stabilized by using BHT, dl- ⁇ -tocopherol as an oil-oxidizing agent and EDTA as a chelating agent
  • retinol which comprises retinol, retinyl acetate, and retinyl palmitate using BHT and BHA, which are oil-soluble oxidizing agents, in order to stabilize retinol by using citric acid and tocopherol
  • BHT and BHA oil-soluble oxidizing agents
  • United States Patent 4,720,353 discloses a water-in-oil type in which retinol is stabilized by using antioxidants such as BHA, ascorbic acid and tocopheryl linoleate.
  • an oil-in-water type emulsion composition containing at least one water-soluble antioxidant, at least one oil-soluble antioxidant, and a chelating gel is disclosed in order to improve the chemical stability of retinoids.
  • European Patent No. 1568106A1 discloses a retinoid A water-soluble antioxidant, an oil-in-water type emulsion composition containing an antioxidant and an free base type imidazole simultaneously present in an oil phase and an aqueous phase of an emulsion, and a chelating agent and an oil-soluble antioxidant.
  • Liposomes are widely used not only in the pharmaceutical field but also in cosmetics and foods. This has the advantage that the physiologically active ingredient can be stably delivered without breakage, and that the physiologically active ingredient can be both lipid-soluble and water-soluble. Liposomes are divided into multilamellar vesicles (MLV) and unilamellar vesicles (ULV). ULV can be divided into Large Unilamella vesicles and Small Unilamella vesicles.
  • MLV multilamellar vesicles
  • ULV can be divided into Large Unilamella vesicles and Small Unilamella vesicles.
  • the size of MLV is 400 ⁇ 3,500nm, the content of liposome is 5 ⁇ 15%, the size of LUV is 100 ⁇ 1,000nm, the content of liposome is 36 ⁇ 65% Is 20 to 50 nm and the amount that can be contained in the liposome is 0.5 to 1.0%.
  • the amount of physiologically active substance that can be contained is the most LUV but unstable, the SLV is the most stable, but the amount that can be contained is small.
  • a cell membrane with a bilayer structure in the form of a multilamellar vesicle is a constituent of all cells and distinguishes the inside and the outside of the cell.
  • the cell membrane is a thin, structured phospholipid bilayer composed of phospholipids and protein molecules, and has selective permeability.
  • Cell surface membranes also have receptor proteins and cell adhesion proteins (membrane proteins).
  • membrane proteins There are other proteins that perform other functions, and these proteins play a very important role in maintaining constant cell function and organization at all times. In animal cells, cell membranes perform this role alone, whereas in yeast, bacteria, and plants, cell walls form the outermost boundaries and provide physical protection.
  • the cell membrane is approximately 10 nanometers thick and can be faintly separated by transmission electron microscopy. Another important role of cell membranes is to maintain cell potential.
  • the basic structure and structure of cell membranes are the same as those of membranes surrounding other cell organelles.
  • the cell membrane is composed of two layers of phospholipid, a phospholipid bilayer, and the cell membrane is described as a flow mosaic structure.
  • a flow mosaic is a two-dimensional flow in which lipids float freely and has a protein acting as a channel or receptor across the cell membrane.
  • This cell membrane model was developed in 1971 by S.J. Singer proposed a lipid protein model, and in 1972 G.L. Nicolson has attached carbohydrates to present its flow properties. Cells vary in the type and amount of lipids to maintain cell membrane fluidity.
  • Cholesterol molecules (in the case of eukaryotic cells) or hopanoids (in the case of prokaryotes) inside the bilayer help to maintain fluidity.
  • all lipid molecules inside the cell membrane are not fluids in the sense that they can diffuse freely. That is, Caveola is relatively fixed in the cell membrane region, and many proteins do not diffuse.
  • the cytoskeleton supports the cell membrane, immobilizes the intracellular proteins, directs them to a specific surface, and also limits the extent to which proteins can move within the cell membrane.
  • the cell membrane surface always shows some structure, not a fluid that moves without form. Synapse is an example of a more structural cell membrane.
  • the new material is contained in or removed from the cell membrane in a number of ways: For example, the fusion of intercellular vesicles not only secretes vesicle internals, but also includes the vesicle membrane material into the cell membrane, which in turn forms vesicles that eventually become vesicles.
  • the cell membrane has a tubular structure, the material from the tube can be absorbed into the cell membrane.
  • molecular exchange is possible in these aqueous materials, even if the concentration of aqueous material in the cell membrane is low (stable cell membrane constituents are not well soluble in water).
  • the cell membrane tension affects the exchange rate of the material.
  • the cell membrane tension and area are related to elasticity and fluidity, respectively. The association with time is called homeostasis, which is how much the area or tension is constant over time.
  • MLV which has a multilamellar structure similar to the cell membrane structure of the skin, has affinity with the skin but is too large to penetrate the skin.
  • Korean Patent No. 10-0750870 discloses a method of extracting seaweed extracts from Salina species of the genus Dunaliella with Dunaliellaceae of Chlamydomonadales neck of Chlorophyceae And a cosmetic composition for improving skin transparency.
  • microalgae extract can be effectively used as an active ingredient of a skin anti-aging cosmetic composition in accordance with this purpose while seeking a substance for improving skin aging, and completed the present invention.
  • the present invention complements the disadvantages of conventional MLV liposomes which have disadvantages when using the above-described high-pressure emulsification device and have skin and affinity but are too large to penetrate the skin, thereby assuring skin-friendly and physiologically active ingredients as much as possible (Algaesome), which is easy to manufacture as well as being able to make microalgae extracts at room temperature to stabilize and penetrate into the skin.
  • the present invention relates to a cosmetic composition for preventing skin aging and containing an algae containing a microalgae extract in a lipid complex.
  • the algae contain a microalgae extract in a lipid complex consisting of phospholipid, phytosphingosine, ceramide 3, cholesterol, phytosterol, beta-sitosterol and squalene.
  • the algae comprises 0.01 to 30.0% by weight phospholipid, 0.01 to 10.0% by weight phytosphingosine, 0.01 to 50.0% by weight of ceramide 3, 0.01 to 30.0% by weight of cholesterol, 0.01 to 30.0% by weight of phytosterol 0.01 To 20.0% by weight, beta to sitosterol 0.01 to 10.0% by weight, squalene 0.01 to 80.0% by weight and microalgae extract 0.01 to 50.0% by weight.
  • the cosmetic composition for anti-aging has at least one of an effect of improving skin wrinkles, a skin elasticity improving effect, and a skin moisturizing effect.
  • the algae are contained in an amount of 0.01 to 100.0% by weight based on the total weight of the total cosmetic composition.
  • the above-mentioned algosa is dissolved at high pressure to form a lipid complex in the form of a translucent gel, and the lipid complex is formed at room temperature.
  • the lipid complex may be prepared by dissolving phosphatidylserine, phytosphingosine, ceramide 3, cholesterol, phytosterol, beta-sitosterol, And a microalgae extract.
  • the microalgae extract is cultured with Dunaliella Salina .
  • the cosmetic composition is selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays And may have a formulation to be selected.
  • the cosmetic of the present invention can contain a microalgae extract in an algae and stabilize it, so that the skin transfer effect is superior to that of a conventional liposome-capturing cosmetic, and thus, a better anti-aging effect of the skin can be obtained.
  • the active ingredient of the microalgae extract is stabilized at the alum size at room temperature, destruction of the active ingredient can be prevented.
  • FIG. 1 shows particle distributions of algae containing microalgae extract prepared in Example 1 according to the present invention.
  • FIG. 2 is a polarized microscope photograph of algae containing the microalgae extract prepared in Example 1.
  • FIG. 2 is a polarized microscope photograph of algae containing the microalgae extract prepared in Example 1.
  • the present invention relates to a skin anti-aging cosmetic composition containing algaesome containing microalgae extract which improves the skin aging phenomenon.
  • prevention of skin aging is used in a broad sense to include wrinkle improvement, elasticity enhancement effect, skin moisturizing effect and skin regeneration effect.
  • the microalgae extract includes all the extracts obtained by culturing microalgae.
  • the microalgae extract may be extracted with one or more extraction solvents selected from the group consisting of various extraction solvents available in the art, for example, purified water, alcohol, butylene glycol, glycerin, acetate, , Preferably ethanol or purified water, and more preferably can be extracted using purified water.
  • extraction solvents selected from the group consisting of various extraction solvents available in the art, for example, purified water, alcohol, butylene glycol, glycerin, acetate, , Preferably ethanol or purified water, and more preferably can be extracted using purified water.
  • microalgae extract according to the present invention includes an ultrasonic extract.
  • Ultrasonic extraction may be carried out by extracting microalgae in an extraction solvent, for example, an extraction solvent selected from the group consisting of purified water, alcohol, butylene glycol, glycerin, acetate, hexane and the like, followed by extraction with an ultrasonic extractor.
  • microalgae refers to a single-celled plant, often referred to as phytoplankton, which is only observable by microalgal microscopy and has no roots, stems, or leaves.
  • phytoplankton a single-celled plant
  • blue-green, yellow, red, and brown, depending on the pigment are typical microalgae such as diatoms and dinoflagellates.
  • a closed photobioreactor system capable of producing an expensive physiologically active substance and culturing at a high concentration was used.
  • the photobioreactor used in the present invention maximizes the surface area per unit volume to efficiently transmit light, selects an effective light source that can increase the utilization efficiency of the total light energy, and transmits light efficiently from the light source to the cell. Thereby maximizing the concentration.
  • algae in the present invention means a case where a vesicle containing a microalgae extract is captured and stabilized.
  • the alges according to the present invention can be used as a main lipid component constituting the cell membrane such as phospholipid, phytosphingosine, ceramide 3, cholesterol Dissolving cholesterol, phytosterol, beta-sitosterol and squalene at high pressure to prepare a lipid complex in the form of a translucent gel; and mixing the lipid complex and the microalgae extract at room temperature . ≪ / RTI >
  • the main lipid components constituting the cell membrane are phospholipid, phytosphingosine, ceramide 3, cholesterol, phytosterol, ⁇ -sitosterol ) And squalene are dissolved at high pressure using a high-pressure emulsifier (Microfludizer) to make a translucent gel-like lipid complex.
  • a high-pressure emulsifier Microfludizer
  • an appropriate amount of the lipid complex is added to an aqueous solution in which the microalgae extract is dispersed at room temperature to obtain an algae containing the microalgae extract of the present invention having a closed double-layer structure.
  • the temperature of the reaction process proceeds at room temperature.
  • the conventional liposome manufacturing process using a high-pressure microfludizer has a disadvantage in that heat stability of temperature-sensitive components can not be secured due to the formation of liposomes at a high temperature (60 ° C or higher).
  • the present invention differs from the use of a high pressure emulsification apparatus for mixing liposome composites, but the use of a high pressure emulsification apparatus for forming liposomes of the prior art.
  • a lipid complex dissolved and mixed in a high-pressure emulsification apparatus is formed, and they form a vesicle in an aqueous solution phase and form an algae by inlaying an effective ingredient, i.e., a microalgae extract, dispersed in an aqueous solution .
  • the content of the microalgae extract may be 0.001 to 50.0% by weight, preferably 0.1 to 20.0% by weight based on the total weight of the algae.
  • the phospholipid constituting the algae is contained in an amount of 0.001-30.0% by weight, preferably 0.1-10.0% by weight, based on the total weight, based on the total weight of the algae.
  • Phytosphingosine is contained in an amount of 0.01-10.0% by weight, preferably 0.1-5.0% by weight, based on the total weight of the algae.
  • Ceramide 3 is contained in an amount of 0.01 to 50.0% by weight, preferably 0.1 to 30.0% by weight based on the total weight of algae.
  • Cholesterol is contained in an amount of 0.01-30.0% by weight, preferably 0.01-20.0% by weight, based on the total weight of the algae.
  • Phytosterol is contained in an amount of 0.01-20.0% by weight, preferably 0.1-10.0% by weight, based on the total weight of the algae.
  • the beta-sitosterol is contained in an amount of 0.01-10.0% by weight based on the total weight of the algae, preferably 0.1-5.0% by weight based on the total weight.
  • Squalene content is 0.01-80.0 wt% based on the total weight of algae, preferably 0.1-50.0 wt% based on the total weight.
  • the average droplet size of the algae thus prepared is 20 to 500 nm, preferably 50 to 200 nm, and most preferably 80 to 150 nm.
  • average droplet size of the above algae exceeds 500 nm, skin penetration and formulation stability are very weak.
  • ALGAESOME may be contained in an amount of 0.01 to 100.0% by weight, preferably 0.1 to 20.0% by weight based on the total weight of the cosmetic composition.
  • the components contained in the cosmetic composition of the present invention may contain, in addition to the microalgae extract, various components which maintain the activity of the biological component, components commonly used in cosmetic compositions such as antioxidants, stabilizers, solubilizers, vitamins , Customary adjuvants such as pigments and flavoring agents, and carriers, and the like.
  • the cosmetic composition containing the alginate containing the microalgae extract of the present invention is not particularly limited in its formulation and may be, for example, softening lotion, astringent lotion, nutritional lotion, eye cream, serum, It can be applied to the formulations of massage cream, cleansing cream, cleansing lotion, cleansing foam, cleansing water, body cleanser, body lotion, body cream, powder, essence, pack, hair tonic, hair treatment, shampoo, rinse, Lt; / RTI >
  • the other ingredients for further enhancing the efficacy in addition to the algae containing the microalgae extract may be selected and mixed without difficulty by those skilled in the art depending on the formulation or use purpose of other cosmetics.
  • the microalgae used for the present invention were Dunaliella Salina from KMMCC (Korea Marine Microalgae Bank), and the medium used for culture was F / 2 medium (Guillard and Ryther 1962) And sterilized at 121 ⁇ ⁇ for 15 minutes.
  • the cultured microalgae were centrifuged at 10,000 rpm for 10 minutes, and the supernatant containing the medium was discarded. Only the microalgae were separated, followed by centrifugation at 10,000 rpm for 10 minutes by adding purified water. The supernatant was discarded, The microalgae of the present invention are obtained. Add purified water to the obtained microalgae and sonicate (1min on, 30seconds off, 22.5kHz) 10 times. After sonication, centrifuge at 10,000 rpm for 10 minutes to separate the supernatant except for the precipitate, and centrifuge again at 10,000 rpm for 5 minutes. The supernatant thus obtained was filtered with a 6 ⁇ m filter to prepare microalgae extract of the present invention.
  • Algaesome has the same contents as in Table 1 below, such as phospholipid, phytosphingosine, ceramide 3, cholesterol, phytosterol, beta-sitosterol ⁇ -sitosterol and squalene were passed through a high-pressure microfludizer three times at a pressure of 1,000 bar to form a translucent gel-like lipid complex.
  • a solution in which a microalgae extract (Preparation Example 1) was dispersed on an aqueous solution was prepared. Next, at the room temperature, the lipid complex was added to the aqueous solution containing the microalgae extract to prepare the microalgae extract-captured algae.
  • the following experiment was conducted to examine the particle size distribution of the algae particles containing the microalgae extract of the present invention.
  • the particle size of the alumina particles was analyzed by using a Zetasizer NS apparatus and the results are shown in the graph of FIG.
  • the average particle size of the obtained algae is 120 nm, which is smaller than that of the liposome prepared using the conventional high pressure emulsifier (microfluidizer), and the homogeneity of the particle size can be improved and the stability can be improved.
  • FIG. 2 shows an SEM photograph of the algae (Preparation Example 2) containing the microalgae extract prepared according to the present invention.
  • Example 1 a composition (Example 1) containing the alginate containing the microalgae extract prepared in Preparation Example 2 and a composition containing the existing liposome containing the microalgae extract (Comparative Example 1) were prepared.
  • Comparative Preparation Example 1 The conventional liposome used in Comparative Example 1 (Comparative Preparation Example 1) was prepared by mixing 3.0 wt% of phospholipid, 10.0 wt% of propylene glycol, 10.0 wt% of ethyl alcohol, 3.0 wt% of mineral oil and 64 wt% of purified water, , And then 10.0% by weight of the microalgae extract was poured into the above-mentioned solution, stirred, and then passed through a high pressure microfludizer at a pressure of 1,000 bar twice.
  • Example 1 Comparative Example 1 Totally 0.2 0.2 Algussa (Preparation Example 2) 10.0 - Liposome (Comparative Preparation Example 1) - 10.0 A Arachidyl alcohol 3.0 3.0 Glyceryl stearate 1.5 1.5 Squalane 5.0 5.0 Hydroxypolydecin 2.0 2.0 Trioctanoin 5.0 5.0 Polysorbate 80 1.0 1.0 Sorbitan stearate 0.5 0.5 Cyclopentasiloxane 3.0 3.0 Tocopheryl acetate 0.2 0.2 BHT 0.05 0.05 B Purified water Balance Balance Concentrated glycerin 4.0 4.0 1,3-butylene glycol 2.0 2.0 Dimethicone / Vinyl Dimethicone Crosspolymer 0.4 0.4 EDTA-2Na 0.05 0.05 Incense, preservative Suitable amount Suitable amount system 100 100 100
  • the skin wrinkle improving effect (mechanical evaluation) of the cosmetic containing the alginate containing the microalgae extract of the present invention was measured.
  • Example 1 was compared to Group B, and Comparative Example 1 was divided into 2X2 cm2 (SKIN VISIOMETER SV400, C + K Electronics GmbH, Germany) with replica of skin wrinkle using a transparent silicone solution while applying for 8 weeks (twice / day, 0.2g / Changes in skin wrinkles were measured.
  • the replica image is three-dimensionally analyzed with a CCD camera, and the average roughness roughness (R z ) of the following formula (1), which is a value obtained by dividing the sum of roughnesses of respective wrinkles (R m : m is an integer of 1 or more)
  • the wrinkle improvement effect was analyzed. Test results for the effect of improving skin wrinkles after 6 weeks are shown in Table 3 below.
  • Example 1 Comparative Example 1 T0 T8 ⁇ R z T0 T8 ⁇ R z Subject 1 129 62 -67 128 86 -42 Subject 2 128 41 -87 130 78 -52 Subject 3 121 38 -83 124 82 -42 Subject 4 132 55 -77 135 94 -41 Subject 5 120 22 -98 115 46 -69 Subject 6 136 43 -93 130 75 -55 Subject 7 128 36 -92 125 78 -47 Subject 8 121 39 -82 118 69 -49 Subject 9 129 43 -86 122 91 -31 Subject 10 125 41 -84 117 78 -39 Average -84.9 -46.7
  • Example 1 the average wrinkle roughness (R z ) after 8 weeks was 84.9 ⁇ m lower for cosmetics containing algae containing microalgae extract (Example 1) (p ⁇ 0.01)
  • the wrinkles were improved by about 81.8% as compared with Comparative Example 1 containing the ordinary liposome containing microalgae extract. From the above results, it can be seen that Example 1 containing algae containing microalgae extract showed a more effective wrinkle-reducing effect than Comparative Example 1 containing a general liposome stabilized with microalgae extract.
  • Example 1 Comparative Example 1 T0 T8 T0 T8 Subject 1 0 3 0 2 Subject 2 0 3 0 One Subject 3 0 3 0 2 Subject 4 0 2 0 One Subject 5 0 2 0 2 Subject 6 0 3 0 2 Subject 7 0 3 0 One Subject 8 0 3 0 3 Subject 9 0 2 0 2 Subject 10 0 3 0 2 DELTA W (T8-T0) 2.7 1.8
  • Example 1 Comparative Example 1 T0 T8 T0 T8 Subject 1 0 3 0 2 Subject 2 0 2 0 3 Subject 3 0 3 0 One Subject 4 0 2 0 2 Subject 5 0 3 0 2 Subject 6 0 2 0 One Subject 7 0 3 0 3 Subject 8 0 3 0 2 Subject 9 0 2 0 3 Subject 10 0 2 0 One DELTA W (T8-T0) 2.5 2.0
  • Example 1 the objective evaluation by the skilled person and the subjective evaluation by the subject were 2.7 and 2.5, respectively, indicating that the effect of improving wrinkles is excellent.
  • Example 1 of the present invention showed an increase of 50% in the objective evaluation of the expert and 25% in the subjective evaluation of the subject compared to Comparative Example 1 in which the conventional liposome containing the microalgae extract was contained. It can be seen that Example 1 containing the algae of the present invention improves the wrinkles very effectively.
  • the skin elasticity enhancing effect of the cosmetic containing the alginate containing the microalgae extract of the present invention was measured.
  • Example 1 was applied to A group, The skin elasticity was measured using a skin elasticity tester (Cutometer SEM 575, C + K Electronic Co., Germany) after applying Comparative Example 1 around the eye area for 8 weeks (twice / day).
  • the test results are shown in the following Table 6 as the value of? R8 (R8 (8 weeks) -R8 (0 weeks)) of Cutometer SEM 575.
  • the R8 value indicates the nature of viscoelasticity of the skin.
  • the cosmetic composition (Example 1) containing the algae containing the microalgae extract of the present invention was superior to Comparative Example 1 containing the existing liposome containing microalgae extract
  • the elasticity increased by 56.7%, indicating a high skin elasticity improvement effect.
  • the skin moisturizing effect of the cosmetic containing the algae containing the microalgae extract of the present invention was measured.
  • Example 1 The cosmetic composition of Example 1 according to the present invention was divided into group A and the cosmetic composition according to Comparative Example 1 was divided into groups B and 20, (2 times / day) for 6 weeks.
  • the transepidermal water loss (TEWL) value was measured using a TEWAMETER TM210 (C + K electronic GmbH, Germany) with a change in the TEWL value ⁇ TEWL with time, and the change was measured using a CORNEOMETER CM 820 PC .
  • the moisture content of the skin was quantified from 0 to 150 by the change of the electrical conductivity according to the moisture content of the skin using the. The test results are shown in Table 7 below.
  • Example 1 containing algae containing microalgae extract was 1.5 g / hm 2 , which means that the transdermal water loss was significantly improved after 6 weeks compared to Comparative Example 1.
  • the corneometer value obtained by measuring the moisture content of the skin showed that the skin moisturizing effect of Example 1 was increased by 28.0% than that of the Comparative Example.
  • the skin penetration effect of the cosmetic composition containing the alginate containing the microalgae extract of the present invention was measured.
  • Human normal fibroblast 1 ⁇ 10 5 cells / ml Cells were injected into a collagen gel structure similar to the fibrous tissue of the human body.
  • DE Dermal Equivalent
  • Example 1 and Comparative Example 1 of the present invention were applied to artificial dermal tissue of the uppermost layer and cultured for 4 hours. Then, in Example 1 (Example 1) in which the epidermis and dermis of the artificial skin were permeated through DMEM medium containing no carti- And the amount of carotenoid in the microalgae extract contained in the liposome of Comparative Example 1 was analyzed by TLC. At this time, carnitoid was added to the medium so that the carnitide content in the microalgae extract was 200 ⁇ g / 1 ml, and the algae of Example 1 and the liposome of Comparative Example 1 were prepared so that the microalgae extract contained 10.0% of the total weight. Test results for the skin penetration effect are shown in Table 8 below.
  • the formulation stability of the cosmetic composition containing the algae containing the microalgae extract of the present invention was measured.
  • Example 1 and Comparative Example 1 of the present invention were placed in an opaque glass container in a thermostat kept constant at 45 DEG C and stored for 12 weeks, and the completely shaded After storage for 12 weeks in an opaque glass container in a refrigerator, the extent of discoloration and degree of discoloration were compared. The results are shown in Table 9 below. At this time, the degree of product separation and discoloration was classified into the following six grades and evaluated.
  • Example 1 containing algae was stable at 4 < 0 > C and 45 < 0 > C with no discoloration or separation symptoms, whereas Comparative Example 1, which contained normal liposomes, But it is not stable at 45 °C.
  • Example 1 and Comparative Example 1 Twenty subjects (mean age 26.7 years, age range 18 to 30 years) were subjected to a skin patch test on the upper limb using Haye's Test Chamber of Example 1 and Comparative Example 1 of the present invention. Patients with psoriasis, eczema, other skin lesions, pregnant, lactating or contraceptive, and antihistamines were excluded from the study.
  • the test site is wiped with 70% ethanol and dried, and 15 ⁇ g of each of Example 1 and Comparative Example 1 is dropped on the chamber, and then placed on the upper part of the test site to be fixed. After applying the patch for 24 hours and removing the patch, mark the test area with Marking Pen and observe the test area after 24 hours and 48 hours, respectively. The determination was carried out 24 hours and 48 hours after the removal of the patch, and the skin reaction was judged according to the rules of the International Contact Dermatitis Research Group (ICDRG) shown in Table 10 below. The test results are shown in Table 11 below as skin stability.
  • both the example 1 and the comparative example 1 have a mean degree of irritation of 0 and are a safe cosmetic without irritation to the skin.
  • composition of the present invention is not intended to be limited to the following formulation examples.
  • Example 2 10.0 Cetostearyl alcohol 2.0 Glyceryl stearate 1.5 Trioctanoin 5.0 Polysorbate 60 1.2 Sorbitan stearate 0.5 Squalane 5.0 Liquid paraffin 3.0 Cyclomethicone 3.0 Viechti 0.05 Delta-tocopherol 0.2 Concentrated glycerin 4.0 1,3-butylene glycol 2.0 Xanthan gum 0.1 EDITEE-2 EN 0.05 Incense, preservative a very small amount Purified water to 100

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
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  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Birds (AREA)
  • Biotechnology (AREA)
  • Engineering & Computer Science (AREA)
  • Dermatology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
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  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Cosmetics (AREA)

Abstract

La présente invention concerne une composition cosmétique pour prévenir le vieillissement de la peau, contenant un Algasome comprenant, au sein de ce dernier, un extrait de micro-algues. L'Algasome de la présente invention comprend un extrait de micro-algues retenu dans un complexe lipidique comprenant un phospholipide, une phytosphingosine, un céramide 3, du cholestérol, un phytostérol, du bêta-sitostérol et du squalène, ce qui permet un excellent effet de transfert à la peau. Par conséquent, une composition cosmétique contenant un Algasome comprenant un extrait de micro-algues, selon la présente invention, fait preuve d'excellent effets de réduction des rides de la peau, d'amélioration de l'élasticité de la peau et d'hydratation.
PCT/KR2018/009805 2018-01-11 2018-08-24 Composition cosmétique contenant un algasome comprenant, au sein de ce dernier, un extrait de micro-algues WO2019139214A1 (fr)

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KR1020180003862A KR102008870B1 (ko) 2018-01-11 2018-01-11 미세조류 추출물을 내포하는 알게좀을 함유하는 화장료 조성물
KR10-2018-0003862 2018-01-11

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Cited By (1)

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US11400043B2 (en) 2019-12-10 2022-08-02 Mary Kay Inc. Cosmetic composition

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KR102594647B1 (ko) 2023-07-19 2023-10-27 주식회사 포러스젠 미세조류 혼합 추출물을 유효성분으로 포함하는 기능성화장료 조성물

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