Classifications

• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
  • C12N1/14—Fungi; Culture media therefor
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
  • A01G18/00—Cultivation of mushrooms
  • A01G18/60—Cultivation rooms; Equipment therefor
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
  • A01G18/00—Cultivation of mushrooms
  • A01G18/60—Cultivation rooms; Equipment therefor
  • A01G18/62—Racks; Trays
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
  • A01G18/00—Cultivation of mushrooms
  • A01G18/60—Cultivation rooms; Equipment therefor
  • A01G18/69—Arrangements for managing the environment, e.g. sprinklers
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
  • C12M23/00—Constructional details, e.g. recesses, hinges
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
  • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
  • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
  • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
  • C12M29/24—Recirculation of gas
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
  • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
  • C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi

Definitions

• This invention relates to methods to create a biomaterial of increased homogeneity, strength and density as compared to the mycological biopolymer described in published US Patent Application US 2015/0033620 (A).
• the environmental conditions for producing the mycological biopolymer product i.e. a high carbon dioxide (C0 2 ) content (from 5% to 7% by volume) and an elevated temperature (from 85°F to 95°F), prevent full differentiation of the fungus into a mushroom. There are no stipe, cap, or spores produced.
• the elevated temperature accelerates tissue production.
• the biopolymer product grows into the void space of the tool, filling the space with an undifferentiated mycelium chitin-polymer, which is subsequently extracted from the substrate and dried.
• the invention allows for the production of a tough, pliable material that could be used to replace leather, leather-like materials, textiles and high density and strength foams in many applications such as upholstery, apparel/fashion, military gear, athletic gear, and footwear.
• the invention involves growing a mycological biopolymer under conditions of directed airflow, depositing moisture and solutes, such as minerals, on the surface of the growing organism, growth through a scrim or lofted non-substrate matrix, and fluctuation of the humidity profile throughout growth to induce more homogenous material and produce a range of material densities.
• the mycological biopolymer product consists entirely of fungal mycelium.
• One embodiment of the invention is the placement of contained inoculated growth media used to produce mycological biopolymer within a growth enclosure equipped to deliver a directed airflow across at least one of the surfaces of the growth media.
• the method of growing a biopolymer material comprises the steps of providing a plurality of containers, each of which defines a cavity containing a growth media comprised of nutritive substrate and a fungus; placing the containers in a closed incubation chamber; maintaining the incubation chamber with a predetermined environment of humidity, temperature, carbon dioxide and oxygen sufficient to produce a mycelium biopolymer while preventing full differentiation of said fungus into a mushroom; directing flows of air containing a high carbon dioxide content through the incubation chamber for passage over the growth media in each container; and incubating the growth media in each container for a period of time sufficient for the fungus to digest the nutritive substrate and produce a mycelium biopolymer consisting entirely of fungal mycelium in each container.
• Each container may be placed within the incubation chamber within an“airflow box” such that the height of the container interacts with the airflow or each container may be sunk into the airflow box such that the total cross-sectional area of the box can be employed.
• the flows of air are directed into the closed incubation chamber laterally of the containers or perpendicularly of the containers.
• a second embodiment of the invention employs the controlled deposition of moisture and minerals on at least one of the growing surfaces to induce homogeneity with a range of densities based on the moisture and mineral deposition volume.
• the method of growing a biopolymer material comprises the steps of providing a plurality of containers, each of which defines a cavity containing a growth media comprised of nutritive substrate and a fungus; placing the plurality of containers in a closed incubation chamber; maintaining the incubation chamber with a predetermined environment of humidity, temperature, carbon dioxide and oxygen sufficient to produce a mycelium biopolymer while preventing full differentiation of said fungus into a mushroom; distributing a mist through the incubation chamber for passage over the growth media in each container; and incubating the growth media in each container for a period of time sufficient to produce a mycelium biopolymer in each container.
• the mist includes moisture and a solute, such as minerals.
• a third embodiment of the invention involves the growth of a mycological biopolymer through a scrim or lofted non-substrate matrix that is in direct contact or elevated above the substrate growth surface and grown in a container without the use of a lid.
• a fourth embodiment employs the fluctuation of the percent humidity at time periods of growth throughout the duration of the cycle in order to induce a higher density material of increased homogeneity.
• a fifth embodiment uses specific air flow rates to achieve a range of aerial mycelium densities and mechanical performances.
• the mycological biopolymer is grown from a nutritious substrate, and grows into a panel at a dry density of 0.5 to 4 pounds per cubic foot.
• the localized environmental conditions i.e. high carbon dioxide air, moisture deposition and temperature, must be homogenous, except for the embodiment using a scrim or lofted non-substrate matrix, in order to achieve uniform growth within each panel and throughout the larger growing chamber.
• the lid on the container is removed and the localized environmental conditions are homogenized via airflow.
• airflow allows for growth from the full surface of the growth container and helps to improve the homogeneity and uniformity of the tissue grown. This may be attributed to the airflow facilitating the delivery of humidity, water and solutes, such as minerals, to the growing tissue, elimination of microenvironments, and/or increased mechanical force.
• the growth environments used in the production of edible mushrooms, both specialty and Agaricus currently employ the use of some uncontrolled airflow through the growth chambers for heating, cooling, of gassing carbon dioxide produced by the growing mushrooms or introducing oxygen into the growing chamber. This differs from the airflow technology employed to prevent any and all differentiation of the fungus into a fruiting body that makes an edible mushroom while providing a uniform environment to grow mycological biopolymer
• airflow within the cultivation of mushrooms is directed at removing metabolic byproducts such as carbon dioxide and other volatiles, and is intermittent in nature.
• the airflow employed to grow mycological biopolymer is directed at providing a consistent homogenization of the incubation environment without localized variations that has sufficiently controlled parameters (e.g., high carbon dioxide) such that the mycelium cannot differentiate into a mushroom.
• the airflow velocity provides a directed force that modulates the structure of the aerial mycelium, impacting density.
• the growth environments used in the production of edible mushrooms can employ the use of an airflow through the growth chambers, the air flow is indirect and part of a recirculating system for humidification of the environment.
• the airflow is not directed across the surface of the growth media as is the case in accordance with the invention.
• Fig. 1A illustrates photographs of the top surfaces of panels grown in a direct, high airflow environment with minimal differentiation in tissue morphology in accordance with the invention
• Fig. 1 B illustrates photographs of the top surfaces of panels grown in an indirect, low airflow environment with highly differentiated tissue
• Fig. 1C illustrates photographs of the top surfaces of panels grown in a zero- airflow environment and resulting in highly differentiated tissue and reduced aerial growth
• Fig. 2 illustrates a chart of treatment versus density in accordance with the invention
• Fig. 3A1 schematically illustrates a lateral airflow system in accordance with the invention
• Fig. 3A2 illustrates a perspective view of an air box used for the incubation of two containers in accordance with the invention
• FIG. 3B schematically illustrates a modified lateral airflow system in accordance with the invention
• FIG. 3C schematically illustrates another modified lateral airflow system in accordance with the invention.
• Fig. 4A schematically illustrates a perpendicular airflow system for passing air over the surface of the growth medium in accordance with the invention
• Fig. 4B illustrates a photograph of the top surface of a panel grown in the system of Fig. 4A;
• Fig. 4C schematically illustrates the air flow patterns over a growth medium in the system of Fig. 4A;
• Fig. 5A schematically illustrates a mist distribution system in accordance with the invention.
• Fig. 5B schematically illustrates an indirect air flow system for recirculation of humidified air not in accordance with the invention.
• the method of growing a biopolymer material employs a closed incubation chamber 10 having a plurality of vertically spaced apart shelves 11 and transparent front walls (not shown) for viewing the interior of the chamber 10.
• an air flow system 12 is connected with the chamber 10 for directing air flows laterally across the chamber 10 as indicated by the arrows 13 from one side of the chamber 10 to and through the opposite side of the chamber 10.
• the air flow system 12 includes a manifold M in the upper part of the chamber 10 for distributing humidified air across the top of the chamber 10 for cascading down the shelves 11 until being recirculated on the bottom right for re-humidification.
• Each shelf 11 of the chamber 10 is sized to receive an air box B that contains two containers 14 each of which contains a growth media 15 comprised of nutritive substrate and a fungus.
• each container 14 is in the form of a rectangular tray with an open top to define a cavity of a size of 11.5 inches by 18.5 inches with a 1 inch lip around the entire container that extends externally outwardly of the cavity.
• Each container is placed within the air box B.
• the containers 14 are constructed from a sufficiently rigid, non-reactive material, such as polycarbonate, and the orifice of the container is such that it is paired with the airflow device to achieve the desired air flow rates.
• the length of the container along with the airflow rates dictate the consistency of this flow, and the entrance length before the airflow reaches the growing part is impart to control the laminar or turbid nature of the flow.
• the containers can include ramps, fairings, such as airfoils, or baffles, to assist in homogenizing the flow.
• the air box B is of rectangular shape that receives the growth trays 14 and has an open side 16 in one end face and a smaller orifice 17 in an opposite end face.
• the air flow system 12 includes a fan 12’ situated at the orifice 17 of each air box
• the fan 12’ may be positioned at the open side 16 of the air box B to push air over the growth media 15.
• the humidified air cascading down from the manifold M passes into and through each air box B via the orifices 16, 17.
• the growth media 15 comprises:
• the incubation chamber 10 is maintained with a predetermined environment of humidity, temperature, carbon dioxide and oxygen. Specifically, the chamber 10 is maintained at 99% relative humidity (RH), 5% C0 2 , and a fluctuating temperature of from 85°F to 90T during the step of incubating.
• RH relative humidity
• C0 2 5% C0 2
• the incubation chamber 10 i.e. growth enclosure, can be open on one end and on the other can be outfitted with fans or apparatuses for moving air over the containers 14 in a lateral direction as indicated by the arrows 13 either by pulling or pushing air at speeds ranging from 5 CFM to 10,000 CFM steadily or in a pulsing fashion.
• the incubation chamber 10 can be within a larger incubation chamber (not shown) that is able to maintain environmental conditions including humidity, temperature, carbon dioxide and oxygen.
• the shape and construction of the incubation chamber 10 can be specially crafted to assist in directing the air flow and laminar or turbid characteristics of the air flow.
• the flows of air are generated by fans outfitted to the incubation chamber 10 and are directed over the containers 14 and back into the greater incubation space.
• a pair of panels 17 produced in accordance with the above method consists entirely of fungal mycelium and show minimal differentiation in tissue morphology.
• Airflow rates of 100 cubic feet per minute at a constant RH of >99% resulted in tissue with a dry density of 1.98 pcf and a tensile strength of 17.5 psi. These panels offered a high degree of consistency.
• pairs of panels produced under conditions without a directed airflow were characterized in having highly differentiated tissue.
• pairs of panels grown in a zero-airflow environment were characterized in having highly differentiated tissue and reduced aerial growth
• the incubation chamber 10 may be constructed with vertically spaced apart shelves 11 (or racks) and may be enclosed by sheeting (not shown) for cooperation with containers 14 of extended length such that each shelf 11 receives an air box B with only a single container 14.
• the incubation chamber 10 is outfitted with a lateral airflow system 12’ having fans fitted to the chamber 10’ to direct airflow from the incubation environment through the air boxes B and over the containers 14 and back into the greater incubation space as indicated by the arrows 18.
• the incubation chamber 10’ may have open shelves 11 on which containers 14 with growth medium 15 are placed without using air boxes.
• the incubation chamber 10’ is outfitted with a lateral airflow system having fans (not shown) located on the right-hand side, as viewed, of the chamber 10’ for pulling air flows through and out of the chamber 10’ while passing laterally over the containers 14.
• the growth of the mycological biopolymer may be effected by passing the airflows perpendicularly of the containers 14.
• the enclosed incubation chamber 10 may be constructed with one or more air flow devices (not shown) positioned above the nutritive media 15 to push or pull conditioned air over the growing mycelium.
• the air flow device 12 as in Fig. 3A1 is either held static at a desired height above the growth container 14’ or modulated on linear actuators (not shown) through the course of growth.
• each container 14’ is positioned on each shelf 11 within the incubation chamber 10” and each container 14’ is provided with vertical standoffs 18 that space a cover 19 (roof) from a container 14’.
• the vertical standoffs 18 are fabricated from a non-reactive substance, such as polyvinylchloride (PVC), and are sufficiently rigid to resist the forces of the airflow device.
• PVC polyvinylchloride
• the incubation chamber 10 can be open on one end and on the other can be outfitted with fans or apparatuses for moving air over the containers 14’ in a direction perpendicular to the growing surface as indicated by the arrows 13” either by pulling or pushing air at speeds ranging from 5 CFM to 10,000 CFM steadily or in a pulsing fashion.
• the incubation chamber 10 can be within a larger incubation chamber (not shown) that is able to maintain environmental conditions including humidity, temperature, carbon dioxide and oxygen.
• a panel of mycological biopolymer produced in the incubation chamber 10 may be characterized in having a concentration of mycelium below the airflow device as the air was pulled up over the growing surface as indicated in Fig. 4C as opposed to across the growing part in Fig. 1A. As indicated in Fig. 4B, where airflow device pulled the air upwardly from a central region of the growth medium, the growing mycelium was concentrated in the central region of the panel.
• the shape and design of the growth enclosure can be specially crafted to
• panels can be grown for 4 to 14 days within the incubation chamber 10”.
• Air movement can be used to mold and structure the material into particular shapes and patterns during growth for a final product that is shaped using airflow.
• Step 6 above pulled horizontal airflow velocity (>175 cfm) creates a dense scalloped pattern.
• Vertical airflow creates structures below the airflow device presenting a morphology that parities the airflow (pulled upward like a stalagmite). Pushing creates wave patterns opposing the airflow (160 CFM). Proximity to the airflow device and the pattern of airflow generates tissue patterns that mimic the flow.
• the moisture and solute content of the growth media has been found to directly relate to the density of the material being grown.
• Fig. 2 shows three other substrate varieties in comparison to the corn stover material at 4 different moisture contents. This resulted in variations in the final product density, which higher moisture contents resulting in lower density tissue.
• Tukey Kramer is a mean (average) comparison test that determines the significant difference between tests. The 0.05 is the confidence interval, so there is a 95% confidence in the relationship between the data.
• the ability for fungal cells to fill the void space is dependent on the water and solutes available to the organism during growth. The more water available, the more aggressively the organism can expand, causing the density of the material to drop.
• an enclosed incubation chamber 20 is fitted with a mist distribution system 21 so that moisture and solutes can be applied to the growing tissue through a number of avenues for the purpose of producing a range of material densities in the produced mycological biopolymer.
• the incubation chamber 20 has a plurality of vertically spaced apart shelves 21 and transparent front walls (not shown) for viewing the interior of the chamber 20.
• the incubation chamber 20 is sized to receive a plurality of containers 14, each filled with a growth media 15.
• the incubation chamber 20 can be placed within larger incubation chambers that are able to maintain uniform environmental conditions including humidity, temperature, carbon dioxide and oxygen.
• the mist distribution system 21 is positioned to deliver moisture and solutes, such as minerals, to the top of the growing tissue in each container 14 and can also be used to control the material density and regulate the homogeneity of the material.
• This material is comprised of aerial hypha growing up and out of a nutritious space into a non-nutrient environment.
• the organism employs the use of turgor pressure to regulate the extension of the hyphae at the apex, or hyphal tip.
• regulating the amount, distribution and/or droplet size of available moisture and solutes deposited across the top surface of the growing material can control the osmotic gradient created within the hyphae and subsequently, its growth rate and pattern of colonization.
• Solutes are any agent that can cause an osmotic potential. RO (reverse osmosis) or distilled water are free of such agents. Other solutes could include proteins, carbohydrates, polymers, and minerals.
• a solute is a material that induces an osmotic potential within a solution.
• a solute can be a mineral, a carbohydrate, a protein, or lipid. Concentrations of a solute on one side of a membrane, such as a cell membrane and/or wall, will drive a potential across the membrane if the solution on the opposing side of the membrane has a
• Moisture and solute deposition can be employed to achieve specific material densities and increase material homogeneity.
• Moisture and solutes can be distributed across the growing surface of the growth media using a bath of water outfitted with a“humidifying puck” that atomizes the water into vapor or mist.
• A“humidifying puck” is an ultrasonic humidifier which produces low quality, high liquid content, droplets of a size range of 5 to 22 microns.
• the liquid water droplet, opposed to vapor, is important as the droplet can carry a solute. The same is true for sprays or bubblers, but cannot be achieved with steam. Steam can be used to regulate humidity, but not as a substitute for water carrying the solutes.
• This mist can be distributed across the surface of the growth media using indirect airflow from a fan or similar apparatus or by a spray nozzle that can be outfitted with compressed air or other means of expelling the moisture out of the nozzle and directed at the growing surface of the growth media.
• the amount of moisture and minerals, the distribution, and the droplet size can be regulated to produce a homogenous mycelium biopolymer of varying densities.
• Fluctuation of the percent humidity during the growth cycle can be employed as a method to increase the density and homogeneity of the material.
• the humidity was held static throughout the duration of the growth cycle to achieve material growth.
• a moist environment is generally necessary for fungi to grow aggressively.
• a desiccating environment When a desiccating environment is encountered, many species of fungi have developed methods to protect themselves against moisture loss.
• aerial hyphae a localized high humidity environment is necessary to allow for continued expansion and prevent collapse of the hyphae towards the growing surface. Fluctuation of the humidity in the growth chamber can be used to trigger physiological responses of the organism to a desiccating environment as well as to manipulate the aerial hyphal growth in order to achieve the desired material characteristics.
• a system design allowing for the controlled deposition of mist onto the growing material without the use of airflow was prototyped and tested employing the incubation chamber of Fig. 5A.
• This misting system prototype evenly distributed an equivalent volume of mist onto the growing material as a control high airflow system.
• the misting system used a SF1010SS siphon fed atomizing nozzle, or“atomizer” to expel a fan shaped spray of fine water droplets, equivalent in size to MycoFlexTM control technology as employed in the methods described in US 2015/0033620, across the growing surface of the experimental parts without the use of direct airflow.
• the atomizer misting system was set up with the nozzle positioned 26.5 inches in from the incubator wall to the right side of the target growth surface.
• the nozzle was affixed at a 45-degree angle to the shelf 11 above the target container 14 and rotated 90-degrees, resulting in a vertically oriented fan-shaped spray pattern.
• the target total volume of moisture of 0.28 microsiemens per centimeter (uS/cm) per minute plus/minus seven microsiemens per centimeter (uS/cm) as well as target deviation in moisture across the panel surface of 0.00014 g/min was achieved using a misting paradigm of 2.4% time misting over a 1 minute period.
• the target volume was based on TDS values collected for the direct, high airflow incubations system of Fig. 3A1.
• Humidification of this system was achieved by the moisture input into the system via the atomizer.
• Two control incubators were run simultaneously using the standard biopolymer humidification system and environmental conditions.
• One control incubator was set up using the standard direct, high airflow box system and the humidification recirculation system (Fig. 3A1) while the other was equipped with only the low, indirect airflow used for the recirculation of humidified air (Fig. 5B). All three incubators were set to standard biopolymer environmental conditions of 99% RH, 5% C02 and fluctuating temperature of 85-90 degrees Fahrenheit for nine days of growth.
• a panel grown by this technique may be characterized in having“bulbs” or bundles of mycelium fibers from 0.1 to 1 inch in diameter and in having discrete dense regions predominantly void of connective tissue.
• a panel grown by this technique may be characterized in having“bulbs” or bundles of mycelium fibers equal to or greater than 0.6 inches, for example of from 0.6 to 4 inches in diameter. By comparison, the“bulbs” of mycelium fibers on the panel of Fig. 1C are less than 0.6 inches.
• the panel of Fig. 1 B is characterized in that the connective tissue is minor and results in a homogeneous aesthetic but heterogeneous performance. This means that, although the surface looks smooth, the mechanical performance may vary through the section of the part.
• the high, direct airflow growth environment resulted in panels that were significantly more homogenous, with minimal differentiation throughout the panels (Fig. 1A).
• Tests were conducted to determine the effect of regulating the moisture and minerals within a substrate (growth media) prior to incubation in an enclosed incubation chamber with respect to the density of a produced panel of mycological biopolymer.
• the result of the test was that the amount of moisture and minerals within the growth media prior to placement in the incubation chamber can be regulated to produce a homogenous panel of mycological biopolymer of a desired density.
• moisture contents of 65% on corn stover substrate resulted in densities of 1.7 pcf
• moisture contents of 55% resulted in densities of 2.7 pcf.
• the mycological biopolymer may be grown through a scrim or lofted non-substrate matrix.
• the scrim or lofted non- substrate matrix is either organic or inorganic in nature and offers sufficient porosity such that the mycelium can infiltrate the material.
• the scrim or lofted non-substrate matrix is positioned on or above the nutritive substrate and the entire assembly is incubated in one of the configurations above.
• the scrim or lofted material serves as reinforcement to the mycelium, a means of oriented and directing tissue growth, a method for consistently removing the grown tissue from the nutritive substrate, or a combination thereof.
• the fluctuation of the percent humidity at time periods of growth throughout the duration of the cycle is employed in order to induce a higher density material of increased homogeneity.
• the relative humidity is sustained at a high percentage during the period of aerial mycelium induction, which can begin between day 0 and 5 of growth. Once induced, the humidity is reduced to less than 98% for a period of 4 to 72 hours to induce a densification of the apical tissue. The humidity can then again be elevated to induce newly differentiated growth to provide a range of density, tissue morphology, and orientation through the cross-section of the product. This can be repeated as many times as necessary to garner desired variations in performance through the mycological foam.
• specific air flow rates are used to achieve a range of aerial mycelium densities and mechanical performances.
• the air flow can be set at a constant rate, such that the air flow velocity is passively modulated at the tissue grows, or the rate can be adjusted through the course of incubation to deliver a constant rate over the growing tissue.
• Higher airflow rates have demonstrated the production of denser tissues, while lower airflow rates result in a higher loft of tissue that is less dense when dried.

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• 2018
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